CN2075203U - Liquid injecting device for biological ferment - Google Patents
Liquid injecting device for biological ferment Download PDFInfo
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- CN2075203U CN2075203U CN 90213799 CN90213799U CN2075203U CN 2075203 U CN2075203 U CN 2075203U CN 90213799 CN90213799 CN 90213799 CN 90213799 U CN90213799 U CN 90213799U CN 2075203 U CN2075203 U CN 2075203U
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- valve
- inoculation
- liquid
- injecting device
- liquid injecting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Genetics & Genomics (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a liquid injecting device for biological ferment, which comprises a liquid bottle, a soft tube, a threeway pipe and a valve. The utility model can be used for production processes, such as biological ferment inoculation, feeding, fluid infusion, etc. The utility model has the advantages of simple structure, convenient fabrication, reliable performance of the device, and low cost. The inoculation and fluid infusion can be completed at anytime of the production. The rate of theoretical contamination is zero. The inoculation and fluid infusion can be repeatedly carried out for a plurality of times in the same production period. The liquid injecting device for biological ferment can normally operate under the condition that the tank body is under pressure. The competitor is not easy to be polluted, and the culture is not easy to be scalded. The disadvantages of small amount of inoculation and difficult inoculation of the hypha are overcome. The investment is few, and the operation is convenient. The liquid injecting device for biological ferment is easy to realize the industrialized generalization.
Description
The utility model relates to a kind of priming device, particularly the biological fermentation priming device.
Two kinds of approach are adopted in present domestic biological fermentation inoculation: the one, adopt to connect the inoculation of clock pin pressure reduction, and be applicable to the situation that inoculum size is less, the strain liquid granularity is less; The 2nd, adopt the opening flame inoculation of inoculation bottle, the inoculation mouth opens wide during operation, and the seeding tank tank pressure is zero, and the inoculation mouth has flame protection, and careless manipulation is easily scalded bacterial classification or is caused microbiological contamination, causes the bad growth of bacterial classification or cultivates failure, thereby influence whole fermentative production.
At present both at home and abroad biological fermentation is reinforced, the fluid infusion approach is summarized two kinds: a kind of is feed liquid after the sterilization in the feed supplement jar to be mended into fermentation main body jar by fixing pipeline gradation in batches, but this approach is not suitable for repeatedly feed supplement of a small amount of; Another kind is to utilize the automatic joint control continuous feeding of microcomputer control, see " medicine design " 1984(4) 10-12, " medicine industry " 1981(10) 1-2, data such as Food and Fermentation Industries 1990 (1) 1-4, though this device is very desirable, but the cost height has high input, and suitability for industrialized production is used and is subjected to certain limitation.
The purpose of this utility model will provide a kind of biological fermentation priming device exactly, can be widely used in production processes such as the inoculation of biological fermentation, reinforced, fluid infusion.
The purpose of this utility model is achieved in that fixed tube directly inserts in the liquid bottles, is connected with flexible pipe between fixed tube and the pipe connecting, is respectively equipped with three valves at three mouths of pipe of Y-tube, can control inlet tube, vapour pipe and liquid injection tube respectively.
The utlity model has simple in structurely, easily manufactured, device performance is reliable, and is with low cost, can the random time inoculation in the production, feed supplement liquid, theoretical microbiological contamination rate be zero and can be in the same production cycle characteristics such as repeated multiple times inoculation, feed supplement liquid.Compared with prior art, this device can have normal running under the condition of pressure at tank body, is difficult for dying assorted bacterium and scalds bacterial classification, has overcome the shortcoming that inoculum size is little and mycelium inoculation is difficult, has less investment, and is easy to use, is convenient to characteristics such as industrialization promotion.
Accompanying drawing is a structural representation of the present utility model.
Describe the utility model in detail below in conjunction with accompanying drawing.
One end of fixed tube 2 directly inserts in the liquid bottles 1, and the other end of fixed tube 2 has annular protrusion, can insert in the flexible pipe 3.One end of pipe connecting 4 also has recessed of annular, can insert in the flexible pipe 3, and the other end of pipe connecting 4 has six rib projection and screw threads, can be screwed in the inlet tube 5.Three mouths of pipe at Y-tube 7 are connected with valve 6 respectively, valve 8 and valve 10.Valve 6 is connected with inlet tube 5, and valve 8 is connected with vapour pipe 9, and valve 10 is connected with liquid injection tube 11.Liquid injection tube 11 directly is welded on the tank body.
Embodiment 1:
Liquid bottles 1, fixed tube 2, flexible pipe 3 and pipe connecting 4 are sterilized respectively.After the sterilization, under aseptic technique, will produce with spore liquid or mycelia liquid and pack in the liquid bottles 1, good seal is waited to inoculate.Inlet tube 5, valve 6, Y-tube 7, valve 8, vapour pipe 9, valve 10 all are fixed on the tank body by liquid injection tube 11.Open valve 6,8,10, carry out once real disappearing in company with producing a jar substratum, afterwards, valve-off 10 is waited to inoculate.Under the flame protection, valve-off 6, valve 8 are screwed into pipe connecting 4 in the inlet tube 5, remove flame, and the pending connection position temperature is reduced to room temperature, opens valve 6, and valve 10 injects production main body jar with bacterial classification.Afterwards, valve-off 10 is backed out pipe connecting 4, opens valve 8 inlet tube 5 is washed down.Valve-off 6 and valve 8.
Embodiment 2:
Open 6 pairs of Y-tubes 7 of valve 8 and valve, valve 6 and inlet tube 5 carry out disinfection.With aseptic liquid bottles 1, fixed tube 2, flexible pipe 3 and pipe connecting 4 connect, and are equipped with in the liquid bottles 1 to treat feed supplement liquid after disappearing.Under the flame protection, valve-off 6 and valve 8 are screwed into pipe connecting 4 in the inlet tube 5; remove flame; the pending connection position temperature is reduced to after the room temperature, opens valve 6 and valve 10, and feed liquid is mended in the production main body tank body; afterwards; valve-off 10 removes pipe connecting 4, opens valve 8 inlet tube 5 is washed down; valve-off 6 and valve 8 are to treat feed supplement liquid once more.
Claims (3)
1, a kind of biological fermentation priming device, it is characterized in that fixed tube 2 direct insertions in the liquid bottles 1, be connected with flexible pipe 3 between fixed tube 2 and the pipe connecting 4, three mouths of pipe at Y-tube 7 are connected with valve 6, valve 8 and valve 10 respectively, its valve 6 is connected with inlet tube 5, valve 8 is connected with vapour pipe 9, and valve 10 is connected with liquid injection tube 11, and liquid injection tube 11 is welded on the tank body.
2, a kind of biological fermentation priming device according to claim 1 is characterized in that an end of fixed tube 2 has annular protrusion.
3, a kind of biological fermentation priming device according to claim 1 is characterized in that an end of pipe connecting 4 has annular protrusion, and the other end has six rib projection and screw threads.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 90213799 CN2075203U (en) | 1990-09-07 | 1990-09-07 | Liquid injecting device for biological ferment |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 90213799 CN2075203U (en) | 1990-09-07 | 1990-09-07 | Liquid injecting device for biological ferment |
Publications (1)
Publication Number | Publication Date |
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CN2075203U true CN2075203U (en) | 1991-04-17 |
Family
ID=4893240
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 90213799 Pending CN2075203U (en) | 1990-09-07 | 1990-09-07 | Liquid injecting device for biological ferment |
Country Status (1)
Country | Link |
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CN (1) | CN2075203U (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102337208A (en) * | 2011-10-14 | 2012-02-01 | 无锡赛德生物工程有限公司 | Inoculation port device of fermentation seed tank |
CN107794227A (en) * | 2017-10-26 | 2018-03-13 | 翔天农业开发集团股份有限公司 | A kind of sterile carrying method of the one-to-many pipeline of liquid spawn |
-
1990
- 1990-09-07 CN CN 90213799 patent/CN2075203U/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102337208A (en) * | 2011-10-14 | 2012-02-01 | 无锡赛德生物工程有限公司 | Inoculation port device of fermentation seed tank |
CN107794227A (en) * | 2017-10-26 | 2018-03-13 | 翔天农业开发集团股份有限公司 | A kind of sterile carrying method of the one-to-many pipeline of liquid spawn |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
AD01 | Patent right deemed abandoned | ||
C20 | Patent right or utility model deemed to be abandoned or is abandoned |