CN2811304Y - Automatic seed-receiving device for fermentation system - Google Patents
Automatic seed-receiving device for fermentation system Download PDFInfo
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- CN2811304Y CN2811304Y CN 200520073178 CN200520073178U CN2811304Y CN 2811304 Y CN2811304 Y CN 2811304Y CN 200520073178 CN200520073178 CN 200520073178 CN 200520073178 U CN200520073178 U CN 200520073178U CN 2811304 Y CN2811304 Y CN 2811304Y
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- 238000000855 fermentation Methods 0.000 title claims abstract description 25
- 230000004151 fermentation Effects 0.000 title claims abstract description 24
- 230000005540 biological transmission Effects 0.000 claims description 4
- 238000010899 nucleation Methods 0.000 claims 2
- 230000001580 bacterial effect Effects 0.000 claims 1
- 238000002255 vaccination Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 6
- 238000011081 inoculation Methods 0.000 abstract description 5
- 239000000356 contaminant Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 5
- 238000002054 transplantation Methods 0.000 description 4
- 238000011109 contamination Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
技术领域technical field
本发明涉及生物发酵设备,特指一种发酵系统自动接种装置。The invention relates to biological fermentation equipment, in particular to an automatic inoculation device for a fermentation system.
背景技术Background technique
生物发酵是一种逐级放大的过程,罐容积放大倍数一般为10~15倍,若给出这个范围,环境就不一样,发酵的产物也就会发生变化,不能达到发酵的要求。如10L~100L~1000L这样一个三级发酵,先从摆床上制菌种,然后接种到第一级10L的种子罐内,待发酵好后移种到第二级100L的种子罐内,再发酵,制成种子移到1000L的发酵罐内发酵,最后才能真正出产品,但在每一级移种过程中易污染杂菌。在一个发酵工厂内,会有多套多级发酵设备,发酵设备不能绝对做到一一对应,不能做到充分利用。Biological fermentation is a process of step-by-step amplification, and the tank volume magnification is generally 10 to 15 times. If this range is given, the environment will be different, and the fermentation product will also change, which cannot meet the requirements of fermentation. Such as 10L ~ 100L ~ 1000L such as three-stage fermentation, first prepare bacteria from the swing bed, and then inoculate into the first-stage 10L seed tank, after the fermentation is complete, transplant it into the second-stage 100L seed tank, and then ferment , the seeds are made and moved to a 1000L fermenter for fermentation, and finally the product can be produced, but it is easy to contaminate miscellaneous bacteria in the process of transplanting at each level. In a fermentation factory, there will be multiple sets of multi-stage fermentation equipment, and the fermentation equipment cannot be absolutely one-to-one correspondence, and cannot be fully utilized.
发明内容Contents of the invention
在多套多级发酵设备使用中,为了生产方便、减少杂菌污染,只有相互间移种才能使设备的利用率大大提高,本发明提供了一种能联络各发酵设备、自动灭菌、自动在各发酵设备间配对的移种装置。In the use of multiple sets of multi-stage fermentation equipment, in order to facilitate production and reduce the contamination of bacteria, the utilization rate of the equipment can be greatly improved only by transplanting each other. A paired transplanting device between each fermentation equipment.
一种发酵系统自动接种装置,设有三级罐,主要为一级种子罐、二级种子罐、发酵罐,其特征在于:在一、二级种子罐之间、二级种子罐与发酵罐之间设置了可以中转菌种的小罐,小罐带有蒸汽输入、输出控制阀门,它通过传输管道与一、二级种子罐、发酵罐连通,传输管道两端设置了输入、输出阀门,通过控制阀门可实现多罐之间移种的方向,能根据用户的选择需要在罐与罐之间实现便利移种。An automatic inoculation device for a fermentation system, equipped with three-level tanks, mainly a first-level seed tank, a second-level seed tank, and a fermentation tank, characterized in that: between the first and second-level seed tanks, the second-level seed tank and the fermentation tank A small tank that can transfer strains is installed between them. The small tank has steam input and output control valves. It communicates with the primary and secondary seed tanks and fermentation tanks through the transmission pipeline. Input and output valves are set at both ends of the transmission pipeline. The direction of transplanting between multiple tanks can be realized by controlling the valve, and convenient transplanting between tanks can be realized according to the user's choice.
本装置实现了用户在移种过程中的自动接种、加大了移种的选择度;减少了杂菌污染,使用非常方便。The device realizes the automatic inoculation of the user during the transplantation process, increases the selection degree of transplantation, reduces the contamination of miscellaneous bacteria, and is very convenient to use.
附图说明Description of drawings
图1为发酵系统自动接种装置简图:Figure 1 is a schematic diagram of the automatic inoculation device of the fermentation system:
1-一级种罐输出口阀门;2-中间罐输入口阀门;3-蒸汽输入阀门;4-中间罐输出口阀门;5-二级种罐输入管道;6-二级种罐输入口阀门;7-一级种罐;8-一级种罐输出管道;9-蒸汽输出阀门;10-中间小罐;11-二级种罐;12-发酵罐;1-Outlet valve of primary seed tank; 2-Valve of input port of intermediate tank; 3-Valve input of steam; 4-Valve of output port of intermediate tank; 5-Input pipeline of secondary seed tank; ; 7-first-level seed tank; 8-first-level seed tank output pipeline; 9-steam output valve; 10-intermediate small tank; 11-secondary seed tank; 12-fermentation tank;
具体实施方式Detailed ways
如图1所示,本发明主要设有三级罐——一级种子罐(7)、二级种子罐(11)、发酵罐(12),在一、二级种子罐(7)(11)之间、二级种子罐(11)与发酵罐(12)之间各加了个中间罐10),中间罐(10)的蒸汽输入阀门(3)位于中间罐(10)柱面一侧,蒸汽输出阀门(9)位于另一侧;一级种罐输出管道(8)一端由一级种罐输出口阀门(1)控制,连接至一级种罐罐体中部,并延伸到罐底部,另一端由中间罐输入口阀门(2)控制,连接至中间罐(10);二级种罐输入管道(5)一端由中间罐输出口阀门(4)控制,连接至中间罐(10),并延伸到罐底部,另一端由二级种罐输入口阀门(6)控制,连接至二级种罐(11)顶部;一级种罐输出口阀门(1)、二级种罐输入口阀门(6)、二级种罐输出口阀门和发酵罐输入口阀门都使用带有蒸汽出口。As shown in Fig. 1, the present invention is mainly provided with three-level tank---one-level seed tank (7), two-level seed tank (11), fermenter (12), in one and two-level seed tank (7) (11 ), between the secondary seed tank (11) and the fermenter (12), respectively added a middle tank 10), and the steam input valve (3) of the middle tank (10) is located at one side of the cylinder of the middle tank (10) , the steam output valve (9) is located on the other side; one end of the primary seed tank output pipe (8) is controlled by the primary seed tank output valve (1), connected to the middle of the primary seed tank body, and extends to the bottom of the tank , the other end is controlled by the tundish tank input valve (2) and connected to the tundish tank (10); one end of the secondary seed tank input pipe (5) is controlled by the tundish tank output port valve (4) and connected to the tundish tank (10) , and extend to the bottom of the tank, the other end is controlled by the secondary seed tank input port valve (6), connected to the top of the secondary seed tank (11); the primary seed tank output valve (1), the secondary seed tank input port Valve (6), the outlet valve of the secondary seed tank and the inlet valve of the fermenter all use a steam outlet.
一级种罐(7)和二级种罐(11)间自动移种流程:The automatic transplanting process between the primary seed tank (7) and the secondary seed tank (11):
1)在移种前关闭一级种罐(7)所有输出口阀门(1)、二级种罐所有输入口阀门(6),对移种过程中需要的一级种罐(7)、二级种罐(11)进行高压蒸汽灭菌,维持30min~40min后关闭阀门;打开中间罐(10)上蒸汽输入阀门(3)通入蒸汽,对移种需要使用到的一级种罐输出管道(8)、输入菌种的二级种罐输入管道(5)和中间罐(10)进行灭菌,维持30min~40min后关闭所有蒸汽出入口。1) Close all outlet valves (1) of the first-level seed tank (7) and all input valves (6) of the second-level seed tank before transplanting. The stage seed tank (11) is subjected to high-pressure steam sterilization, and the valve is closed after maintaining for 30min to 40min; the steam input valve (3) on the intermediate tank (10) is opened to feed steam, and the output pipe of the stage seed tank that needs to be used for transplanting (8) Sterilize the input pipeline (5) and the intermediate tank (10) of the secondary seed tank for inputting bacteria, and close all steam inlets and outlets after maintaining for 30 minutes to 40 minutes.
2)根据需要打开输出菌种的一级种罐(7)的输出口阀门(1)、中间罐输入口阀门(2)、中间罐输出口阀门(4)、二级种罐输入口阀门(6);2) Open the outlet valve (1) of the primary seed tank (7), the intermediate tank input port valve (2), the intermediate tank output port valve (4), and the secondary seed tank input port valve ( 6);
3)自动移种。3) Automatic transplantation.
二级种罐(11)和发酵罐(12)之间自动移种同上一、二级种罐间类似。The automatic transplantation between the second-level seed tank (11) and the fermenter (12) is similar to that between the first and second-level seed tanks.
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CN 200520073178 CN2811304Y (en) | 2005-06-30 | 2005-06-30 | Automatic seed-receiving device for fermentation system |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105400696A (en) * | 2015-12-17 | 2016-03-16 | 青州伟格生物科技有限公司 | Production process of microbial agent and device adopted in production process |
CN107299047A (en) * | 2017-08-24 | 2017-10-27 | 北京艾吾丁医药科技有限公司 | A kind of culture transferring control method based on multistage installation for fermenting |
CN110656045A (en) * | 2019-07-08 | 2020-01-07 | 青海华牛生物科技有限公司 | A kind of Lycium barbarum enzyme fermentation and conveying system |
CN115536442A (en) * | 2022-10-31 | 2022-12-30 | 明洋(山东)环境科技有限公司 | Fermentation equipment bacterial sprinkler |
-
2005
- 2005-06-30 CN CN 200520073178 patent/CN2811304Y/en not_active Expired - Fee Related
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105400696A (en) * | 2015-12-17 | 2016-03-16 | 青州伟格生物科技有限公司 | Production process of microbial agent and device adopted in production process |
CN107299047A (en) * | 2017-08-24 | 2017-10-27 | 北京艾吾丁医药科技有限公司 | A kind of culture transferring control method based on multistage installation for fermenting |
CN110656045A (en) * | 2019-07-08 | 2020-01-07 | 青海华牛生物科技有限公司 | A kind of Lycium barbarum enzyme fermentation and conveying system |
CN115536442A (en) * | 2022-10-31 | 2022-12-30 | 明洋(山东)环境科技有限公司 | Fermentation equipment bacterial sprinkler |
CN115536442B (en) * | 2022-10-31 | 2024-02-06 | 明洋(山东)环境科技有限公司 | Strain spraying device of fermentation equipment |
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