CN2811304Y - Automatic seed-receiving device for fermentation system - Google Patents

Automatic seed-receiving device for fermentation system Download PDF

Info

Publication number
CN2811304Y
CN2811304Y CN 200520073178 CN200520073178U CN2811304Y CN 2811304 Y CN2811304 Y CN 2811304Y CN 200520073178 CN200520073178 CN 200520073178 CN 200520073178 U CN200520073178 U CN 200520073178U CN 2811304 Y CN2811304 Y CN 2811304Y
Authority
CN
China
Prior art keywords
tank
spawn
stage
fermentation
automatic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 200520073178
Other languages
Chinese (zh)
Inventor
朱湘临
狄轶娟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu University
Original Assignee
Jiangsu University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangsu University filed Critical Jiangsu University
Priority to CN 200520073178 priority Critical patent/CN2811304Y/en
Application granted granted Critical
Publication of CN2811304Y publication Critical patent/CN2811304Y/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to an automatic spawn-inoculating device for a fermentation system, which is provided with a three-stage tank which mainly comprises a first stage spawn tank, a second stage spawn tank and a fermentation tank. The utility model is characterized in that a small tank capable of transferring spawns is respectively arranged between the first stage spawn tank and the second stage spawn tank and between the second stage spawn tank and the fermentation tank. The small tank is provided with a steam input/output control valve which is communicated with the first stage spawn tank, the second stage spawn tank and the fermentation tank. Both ends of a transmit pipeline are respectively provided with an input/output valve. The spawn transferring direction among multiple tanks can be realized through the control valve, and the convenient spawn transfer can be realized between the tanks according to the user's selection and needs. The automatic spawn-inoculating device realizes the automatic spawn inoculation in the process that the user transfers the spawns, widens the spawn transferring selectivity and reduces the living contaminants. The use is convenient.

Description

发酵系统自动接种装置Automatic inoculation device for fermentation system

技术领域technical field

本发明涉及生物发酵设备,特指一种发酵系统自动接种装置。The invention relates to biological fermentation equipment, in particular to an automatic inoculation device for a fermentation system.

背景技术Background technique

生物发酵是一种逐级放大的过程,罐容积放大倍数一般为10~15倍,若给出这个范围,环境就不一样,发酵的产物也就会发生变化,不能达到发酵的要求。如10L~100L~1000L这样一个三级发酵,先从摆床上制菌种,然后接种到第一级10L的种子罐内,待发酵好后移种到第二级100L的种子罐内,再发酵,制成种子移到1000L的发酵罐内发酵,最后才能真正出产品,但在每一级移种过程中易污染杂菌。在一个发酵工厂内,会有多套多级发酵设备,发酵设备不能绝对做到一一对应,不能做到充分利用。Biological fermentation is a process of step-by-step amplification, and the tank volume magnification is generally 10 to 15 times. If this range is given, the environment will be different, and the fermentation product will also change, which cannot meet the requirements of fermentation. Such as 10L ~ 100L ~ 1000L such as three-stage fermentation, first prepare bacteria from the swing bed, and then inoculate into the first-stage 10L seed tank, after the fermentation is complete, transplant it into the second-stage 100L seed tank, and then ferment , the seeds are made and moved to a 1000L fermenter for fermentation, and finally the product can be produced, but it is easy to contaminate miscellaneous bacteria in the process of transplanting at each level. In a fermentation factory, there will be multiple sets of multi-stage fermentation equipment, and the fermentation equipment cannot be absolutely one-to-one correspondence, and cannot be fully utilized.

发明内容Contents of the invention

在多套多级发酵设备使用中,为了生产方便、减少杂菌污染,只有相互间移种才能使设备的利用率大大提高,本发明提供了一种能联络各发酵设备、自动灭菌、自动在各发酵设备间配对的移种装置。In the use of multiple sets of multi-stage fermentation equipment, in order to facilitate production and reduce the contamination of bacteria, the utilization rate of the equipment can be greatly improved only by transplanting each other. A paired transplanting device between each fermentation equipment.

一种发酵系统自动接种装置,设有三级罐,主要为一级种子罐、二级种子罐、发酵罐,其特征在于:在一、二级种子罐之间、二级种子罐与发酵罐之间设置了可以中转菌种的小罐,小罐带有蒸汽输入、输出控制阀门,它通过传输管道与一、二级种子罐、发酵罐连通,传输管道两端设置了输入、输出阀门,通过控制阀门可实现多罐之间移种的方向,能根据用户的选择需要在罐与罐之间实现便利移种。An automatic inoculation device for a fermentation system, equipped with three-level tanks, mainly a first-level seed tank, a second-level seed tank, and a fermentation tank, characterized in that: between the first and second-level seed tanks, the second-level seed tank and the fermentation tank A small tank that can transfer strains is installed between them. The small tank has steam input and output control valves. It communicates with the primary and secondary seed tanks and fermentation tanks through the transmission pipeline. Input and output valves are set at both ends of the transmission pipeline. The direction of transplanting between multiple tanks can be realized by controlling the valve, and convenient transplanting between tanks can be realized according to the user's choice.

本装置实现了用户在移种过程中的自动接种、加大了移种的选择度;减少了杂菌污染,使用非常方便。The device realizes the automatic inoculation of the user during the transplantation process, increases the selection degree of transplantation, reduces the contamination of miscellaneous bacteria, and is very convenient to use.

附图说明Description of drawings

图1为发酵系统自动接种装置简图:Figure 1 is a schematic diagram of the automatic inoculation device of the fermentation system:

1-一级种罐输出口阀门;2-中间罐输入口阀门;3-蒸汽输入阀门;4-中间罐输出口阀门;5-二级种罐输入管道;6-二级种罐输入口阀门;7-一级种罐;8-一级种罐输出管道;9-蒸汽输出阀门;10-中间小罐;11-二级种罐;12-发酵罐;1-Outlet valve of primary seed tank; 2-Valve of input port of intermediate tank; 3-Valve input of steam; 4-Valve of output port of intermediate tank; 5-Input pipeline of secondary seed tank; ; 7-first-level seed tank; 8-first-level seed tank output pipeline; 9-steam output valve; 10-intermediate small tank; 11-secondary seed tank; 12-fermentation tank;

具体实施方式Detailed ways

如图1所示,本发明主要设有三级罐——一级种子罐(7)、二级种子罐(11)、发酵罐(12),在一、二级种子罐(7)(11)之间、二级种子罐(11)与发酵罐(12)之间各加了个中间罐10),中间罐(10)的蒸汽输入阀门(3)位于中间罐(10)柱面一侧,蒸汽输出阀门(9)位于另一侧;一级种罐输出管道(8)一端由一级种罐输出口阀门(1)控制,连接至一级种罐罐体中部,并延伸到罐底部,另一端由中间罐输入口阀门(2)控制,连接至中间罐(10);二级种罐输入管道(5)一端由中间罐输出口阀门(4)控制,连接至中间罐(10),并延伸到罐底部,另一端由二级种罐输入口阀门(6)控制,连接至二级种罐(11)顶部;一级种罐输出口阀门(1)、二级种罐输入口阀门(6)、二级种罐输出口阀门和发酵罐输入口阀门都使用带有蒸汽出口。As shown in Fig. 1, the present invention is mainly provided with three-level tank---one-level seed tank (7), two-level seed tank (11), fermenter (12), in one and two-level seed tank (7) (11 ), between the secondary seed tank (11) and the fermenter (12), respectively added a middle tank 10), and the steam input valve (3) of the middle tank (10) is located at one side of the cylinder of the middle tank (10) , the steam output valve (9) is located on the other side; one end of the primary seed tank output pipe (8) is controlled by the primary seed tank output valve (1), connected to the middle of the primary seed tank body, and extends to the bottom of the tank , the other end is controlled by the tundish tank input valve (2) and connected to the tundish tank (10); one end of the secondary seed tank input pipe (5) is controlled by the tundish tank output port valve (4) and connected to the tundish tank (10) , and extend to the bottom of the tank, the other end is controlled by the secondary seed tank input port valve (6), connected to the top of the secondary seed tank (11); the primary seed tank output valve (1), the secondary seed tank input port Valve (6), the outlet valve of the secondary seed tank and the inlet valve of the fermenter all use a steam outlet.

一级种罐(7)和二级种罐(11)间自动移种流程:The automatic transplanting process between the primary seed tank (7) and the secondary seed tank (11):

1)在移种前关闭一级种罐(7)所有输出口阀门(1)、二级种罐所有输入口阀门(6),对移种过程中需要的一级种罐(7)、二级种罐(11)进行高压蒸汽灭菌,维持30min~40min后关闭阀门;打开中间罐(10)上蒸汽输入阀门(3)通入蒸汽,对移种需要使用到的一级种罐输出管道(8)、输入菌种的二级种罐输入管道(5)和中间罐(10)进行灭菌,维持30min~40min后关闭所有蒸汽出入口。1) Close all outlet valves (1) of the first-level seed tank (7) and all input valves (6) of the second-level seed tank before transplanting. The stage seed tank (11) is subjected to high-pressure steam sterilization, and the valve is closed after maintaining for 30min to 40min; the steam input valve (3) on the intermediate tank (10) is opened to feed steam, and the output pipe of the stage seed tank that needs to be used for transplanting (8) Sterilize the input pipeline (5) and the intermediate tank (10) of the secondary seed tank for inputting bacteria, and close all steam inlets and outlets after maintaining for 30 minutes to 40 minutes.

2)根据需要打开输出菌种的一级种罐(7)的输出口阀门(1)、中间罐输入口阀门(2)、中间罐输出口阀门(4)、二级种罐输入口阀门(6);2) Open the outlet valve (1) of the primary seed tank (7), the intermediate tank input port valve (2), the intermediate tank output port valve (4), and the secondary seed tank input port valve ( 6);

3)自动移种。3) Automatic transplantation.

二级种罐(11)和发酵罐(12)之间自动移种同上一、二级种罐间类似。The automatic transplantation between the second-level seed tank (11) and the fermenter (12) is similar to that between the first and second-level seed tanks.

Claims (1)

1, a kind of fermentation system automatic vaccination device, be provided with three grades of jars, be mainly first class seed pot (7), secondary seed jar (11), fermentor tank (12), it is characterized in that: between the I and II seeding tank (7) (11), be provided with between secondary seed jar (11) and the fermentor tank (12) can the transfer bacterial classification middle canister (10), canister has steam input, output by-pass valve control (3) (9), it is communicated with I and II seeding tank (7) (11), fermentor tank (12) by transmission pipeline, and the transmission pipeline two ends are provided with input, output valve.
CN 200520073178 2005-06-30 2005-06-30 Automatic seed-receiving device for fermentation system Expired - Fee Related CN2811304Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200520073178 CN2811304Y (en) 2005-06-30 2005-06-30 Automatic seed-receiving device for fermentation system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200520073178 CN2811304Y (en) 2005-06-30 2005-06-30 Automatic seed-receiving device for fermentation system

Publications (1)

Publication Number Publication Date
CN2811304Y true CN2811304Y (en) 2006-08-30

Family

ID=36937690

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200520073178 Expired - Fee Related CN2811304Y (en) 2005-06-30 2005-06-30 Automatic seed-receiving device for fermentation system

Country Status (1)

Country Link
CN (1) CN2811304Y (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105400696A (en) * 2015-12-17 2016-03-16 青州伟格生物科技有限公司 Production process of microbial agent and device adopted in production process
CN107299047A (en) * 2017-08-24 2017-10-27 北京艾吾丁医药科技有限公司 A kind of culture transferring control method based on multistage installation for fermenting
CN110656045A (en) * 2019-07-08 2020-01-07 青海华牛生物科技有限公司 A kind of Lycium barbarum enzyme fermentation and conveying system
CN115536442A (en) * 2022-10-31 2022-12-30 明洋(山东)环境科技有限公司 Fermentation equipment bacterial sprinkler

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105400696A (en) * 2015-12-17 2016-03-16 青州伟格生物科技有限公司 Production process of microbial agent and device adopted in production process
CN107299047A (en) * 2017-08-24 2017-10-27 北京艾吾丁医药科技有限公司 A kind of culture transferring control method based on multistage installation for fermenting
CN110656045A (en) * 2019-07-08 2020-01-07 青海华牛生物科技有限公司 A kind of Lycium barbarum enzyme fermentation and conveying system
CN115536442A (en) * 2022-10-31 2022-12-30 明洋(山东)环境科技有限公司 Fermentation equipment bacterial sprinkler
CN115536442B (en) * 2022-10-31 2024-02-06 明洋(山东)环境科技有限公司 Strain spraying device of fermentation equipment

Similar Documents

Publication Publication Date Title
CN104911096B (en) A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment and technique
CN101353618A (en) Production method and fermentation device of edible fungus liquid strain
US20140273132A1 (en) Process for Inoculating a Bioreactor with Cyanobacteria
WO2016172998A1 (en) Plant bioreactor and use method therefor
CN2811304Y (en) Automatic seed-receiving device for fermentation system
KR101473455B1 (en) Incubator
CN201971826U (en) Biological fermentation and seed transfer device
CN201049944Y (en) Edible mushroom liquid bacterial fermentation device
CN2786131Y (en) Liquid strain incubator
CN101843909B (en) Pollution-free sterilization system for bioreactor
CN107118003A (en) A kind of breeding method of Cordyceps militaris fermented bacterium and application
CN205152208U (en) Bacterium fermentation cultivation's novel inoculation device
CN203057904U (en) Simple fermentation device for liquid strain submerged cultivation
CN101157895B (en) Method for producing edible fungi liquid strains by employing air-lift biology reactor
CN2546401Y (en) Edible mushroom liquid culture transfer pot
CN204079985U (en) A kind of microbial solid fermentation system
CN212025350U (en) Tetramycin fermentation device
CN202968544U (en) Continuous fermentation propagation device for reducing contamination probability
CN103125269A (en) Simple fermentation device for liquid spawn submerged cultivation
CN202415568U (en) Animal cell culture tank
CN1293799C (en) Method and apparatus for producing and inoculating liquid baterial spawn in suspension mode
CN2619944Y (en) Inoculating device for seed pot
CN113201439A (en) Chlorella culture device coupled with silk reeling wastewater treatment and culture method thereof
CN215799515U (en) Simple and convenient inoculation utensil without contamination of bacteria
CN206251655U (en) A kind of seafood mushroom fermentation tank air-breather

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
C19 Lapse of patent right due to non-payment of the annual fee
CF01 Termination of patent right due to non-payment of annual fee