CN103205359B - Sterile culture detection device - Google Patents

Sterile culture detection device Download PDF

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Publication number
CN103205359B
CN103205359B CN201310154604.7A CN201310154604A CN103205359B CN 103205359 B CN103205359 B CN 103205359B CN 201310154604 A CN201310154604 A CN 201310154604A CN 103205359 B CN103205359 B CN 103205359B
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China
Prior art keywords
air
hose
sterile culture
bottle
pyrosphere
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CN201310154604.7A
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Chinese (zh)
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CN103205359A (en
Inventor
朱海
王其龙
施成
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BORUI BIOMEDICINE TAIXING Co Ltd
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BORUI BIOMEDICINE TAIXING Co Ltd
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a sterile culture detection device which comprises an intake hose, an exhaust hose, a seal bottle and a sampling fire ring that is connected with an air branch pipeline in a sleeving manner, wherein the intake hose and the exhaust hose are connected in the seal bottle, and the seal bottle is internally provided with a detection culture medium. According to the sterile culture detection device, a sterile condition of an air system can be found immediately; immediate judgment is achieved; and production losses are reduced.

Description

A kind of sterile culture proofing unit
Technical field
The present invention relates to the sterile culture proofing unit in a kind of microbial cultivation process.
Background technology
Most industrial fermentations all adopt purebred cultivation, require fermenting process can only have production bacterium, have not allowed " miscellaneous bacteria " to pollute.Cause the reason of fermentation microbiological contamination a lot, seed-borne fungi, air carry disease germs, equipment leakage, sterilizing not thoroughly, misoperation or mismanagement be the common reason that causes fermentation microbiological contamination, wherein to carry disease germs be the main reason that causes microbiological contamination to air.Once air system microbiological contamination, can cause a large amount of continuous production batch microbiological contaminations, serious to Influence of production.Therefore strict control microbiological contamination in production process, finds that microbiological contamination reason has very great help to whole production in time.
Summary of the invention
The object of the present invention is to provide a kind of filtrated air sterile culture proofing unit, can find in time whether microbiological contamination of air system.
Technical solution of the present invention is: a kind of sterile culture proofing unit, comprise air induction hose, exhaust hose, air-tight bottle and be socketed on the sampling pyrosphere of air bypass line, air induction hose and exhaust hose are connected in air-tight bottle, and detection substratum is housed in air-tight bottle.
Further, at the bottom of air induction hose is deep into air-tight bottle bottle.
Further, exhaust hose position in bottle is not less than air induction hose.
Further, jagged on sampling pyrosphere.
Again further, fluted on sampling pyrosphere.
Adopt after above technical scheme, the beneficial effect that the present invention reaches is:
By this aseptic proofing unit, can find in time the aseptic condition of air system, accomplish timely judgement, reduce the loss of producing.
Accompanying drawing explanation
Fig. 1 is integral construction figure of the present invention.
Shown in figure: fermentor tank air branch road valve 1, air bypass line 2, sampling pyrosphere 3, handle 4, air induction hose strainer 5, air induction hose 6, air-tight bottle 7, detects substratum 8, exhaust hose 9, exhaust hose strainer 10.
Embodiment
As shown in Figure 1, a kind of sterile culture proofing unit comprises fermentor tank air branch road valve 1, air bypass line 2, and sampling pyrosphere 3, handle 4, air induction hose strainer 5, air induction hose 6, air-tight bottle 7, detects substratum 8, exhaust hose 9, exhaust hose strainer 10.
Air induction hose strainer 5 is connected to the air bypass line 2 that is connected with fermentor tank air branch road valve 1 on air induction hose 6, air induction hose 6 connects the air-tight bottle 7 that detection substratum 8 is housed, air-tight bottle 7 is also connected with exhaust hose 9, and exhaust hose strainer 10 is housed on vapor pipe 9.
Sampling pyrosphere 3 is to be with reeded semicircle steel pipe, and ethanol is housed in groove.The handle 4 of holding with a firm grip on sampling pyrosphere 3, light ethanol, by sampling pyrosphere 3 breach, be socketed in air bypass line 2, open the air branch road valve 1 of fermentor tank, sampling pyrosphere 3 scorching hot air bypass line 2 approximately 5 minutes back and forth up and down, air bypass line 2 is discharged filtrated air; Then take out air induction hose strainer 5, be put into the interior sterilizing of container of sterilizing; Rapidly air induction hose 6 is connected in air bypass line 2 by air induction hose strainer 5; Air after filtration is delivered to after air-tight bottle 7, via detecting substratum 8, detect, substratum 8 in air-tight bottle 7 was cultivated after 24 hours at 37 ℃, and sampling microscopic examination microbiological contamination situation, discharges detected gas outside bottle finally by exhaust hose 9 and exhaust hose strainer 10.After fermentation ventilation, re-use sampling pyrosphere 3, light the ethanol on sampling pyrosphere 3, aseptic technique disconnects air induction hose 6 fast.

Claims (5)

1. a sterile culture proofing unit, is characterized in that: comprise air induction hose, exhaust hose, air-tight bottle and be socketed on the sampling pyrosphere of air bypass line, air induction hose and exhaust hose are connected in air-tight bottle, and detection substratum is housed in air-tight bottle.
2. a kind of sterile culture proofing unit according to claim 1, is characterized in that: described exhaust hose position in bottle is not less than air induction hose.
3. a kind of sterile culture proofing unit according to claim 1 and 2, is characterized in that: at the bottom of described air induction hose is deep into air-tight bottle bottle.
4. a kind of sterile culture proofing unit according to claim 1, is characterized in that: on described sampling pyrosphere, have breach.
5. a kind of sterile culture proofing unit according to claim 1, is characterized in that: described sampling pyrosphere is provided with groove.
CN201310154604.7A 2013-04-28 2013-04-28 Sterile culture detection device Active CN103205359B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310154604.7A CN103205359B (en) 2013-04-28 2013-04-28 Sterile culture detection device

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Application Number Priority Date Filing Date Title
CN201310154604.7A CN103205359B (en) 2013-04-28 2013-04-28 Sterile culture detection device

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CN103205359A CN103205359A (en) 2013-07-17
CN103205359B true CN103205359B (en) 2014-04-16

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109628289A (en) * 2019-01-03 2019-04-16 伊犁川宁生物技术有限公司 The sampler and method of air miscellaneous bacteria in a kind of detection zymotechnique

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2622193Y (en) * 2003-05-27 2004-06-30 华南理工大学 Portable inoculating fire-loop of biological reactor
CN200996016Y (en) * 2007-01-11 2007-12-26 河南利华制药有限公司 Pressurized inoculating pot
CN201834921U (en) * 2010-08-20 2011-05-18 明博医药技术开发（上海）有限公司 Inoculation opening for fermentation tank
CN202359116U (en) * 2011-12-01 2012-08-01 北京大北农科技集团股份有限公司 Sterilization device for aseptic sampling of fermentation tank
CN203212575U (en) * 2013-04-28 2013-09-25 博瑞生物医药泰兴市有限公司 Sterile culture detection device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2622193Y (en) * 2003-05-27 2004-06-30 华南理工大学 Portable inoculating fire-loop of biological reactor
CN200996016Y (en) * 2007-01-11 2007-12-26 河南利华制药有限公司 Pressurized inoculating pot
CN201834921U (en) * 2010-08-20 2011-05-18 明博医药技术开发（上海）有限公司 Inoculation opening for fermentation tank
CN202359116U (en) * 2011-12-01 2012-08-01 北京大北农科技集团股份有限公司 Sterilization device for aseptic sampling of fermentation tank
CN203212575U (en) * 2013-04-28 2013-09-25 博瑞生物医药泰兴市有限公司 Sterile culture detection device

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