CN103205359A - Sterile culture detection device - Google Patents
Sterile culture detection device Download PDFInfo
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- CN103205359A CN103205359A CN2013101546047A CN201310154604A CN103205359A CN 103205359 A CN103205359 A CN 103205359A CN 2013101546047 A CN2013101546047 A CN 2013101546047A CN 201310154604 A CN201310154604 A CN 201310154604A CN 103205359 A CN103205359 A CN 103205359A
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- air
- hose
- sterile culture
- bottle
- pyrosphere
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a sterile culture detection device which comprises an intake hose, an exhaust hose, a seal bottle and a sampling fire ring that is connected with an air branch pipeline in a sleeving manner, wherein the intake hose and the exhaust hose are connected in the seal bottle, and the seal bottle is internally provided with a detection culture medium. According to the sterile culture detection device, a sterile condition of an air system can be found immediately; immediate judgment is achieved; and production losses are reduced.
Description
Technical field
The present invention relates to the sterile culture proofing unit in a kind of microbial cultivation process.
Background technology
Most industrial fermentations all adopt purebred cultivation, require fermenting process that the production bacterium can only be arranged, and have not allowed " assorted bacterium " to pollute.Cause the reason of fermentation microbiological contamination a lot, seed-borne fungi, air carry disease germs, equipment seepage, sterilization not thoroughly, misoperation or mismanagement be the common reason that causes the fermentation microbiological contamination, wherein to carry disease germs be the main reason that causes microbiological contamination to air.In case it is the air system microbiological contamination can cause a large amount of continuous production batch microbiological contaminations, serious to producing influence.Therefore in production process, the strict control microbiological contamination, find that in time the microbiological contamination reason has very great help to whole production.
Summary of the invention
The object of the present invention is to provide a kind of filtrated air sterile culture proofing unit, can in time find whether microbiological contamination of air system.
Technical solution of the present invention is: a kind of sterile culture proofing unit, comprise air induction hose, exhaust hose, air-tight bottle and be socketed on the sampling pyrosphere of air bypass line, air induction hose and exhaust hose are connected in the air-tight bottle, and the detection substratum is housed in the air-tight bottle.
Further, air induction hose is deep at the bottom of the air-tight bottle bottle.
Further, exhaust hose position in bottle is not less than air induction hose.
Further, jagged on the sampling pyrosphere.
Again further, fluted on the sampling pyrosphere.
After adopting above technical scheme, the beneficial effect that the present invention reaches is:
By this aseptic proofing unit, can in time find the aseptic situation of air system, accomplish timely judgement, reduce the loss of producing.
Description of drawings
Fig. 1 is integral construction figure of the present invention.
Shown in the figure: fermentor tank air branch road valve 1, air bypass line 2, sampling pyrosphere 3, handle 4, air induction hose strainer 5, air induction hose 6, air-tight bottle 7 detects substratum 8, exhaust hose 9, exhaust hose strainer 10.
Embodiment
As shown in Figure 1, a kind of sterile culture proofing unit comprises fermentor tank air branch road valve 1, air bypass line 2, and sampling pyrosphere 3, handle 4, air induction hose strainer 5, air induction hose 6, air-tight bottle 7 detects substratum 8, exhaust hose 9, exhaust hose strainer 10.
The air bypass line 2 that air induction hose strainer 5 will be connected with fermentor tank air branch road valve 1 is connected on the air induction hose 6, air induction hose 6 connects the air-tight bottle 7 that detection substratum 8 is housed, air-tight bottle 7 also is connected with exhaust hose 9, and exhaust hose strainer 10 is housed on the vapor pipe 9.
Sampling pyrosphere 3 is the semicircle steel pipes that have groove, and ethanol is housed in the groove.The handle 4 of sampling on the pyrosphere 3 of holding with a firm grip, light ethanol, be socketed on the air bypass line 2 by sampling pyrosphere 3 breach, open the air branch road valve 1 of fermentor tank, sampling pyrosphere 3 is scorching hot air bypass line 2 about 5 minutes back and forth up and down, and air bypass line 2 is discharged filtrated airs; Take out air induction hose strainer 5 then, be put into the interior sterilization of container of sterilization; Rapidly air induction hose 6 is connected on the air bypass line 2 by air induction hose strainer 5; After air after the filtration is delivered to air-tight bottle 7, detect via detecting substratum 8, cultivation is after 24 hours down at 37 ℃ for substratum 8 in the air-tight bottle 7, and sampling microscopic examination microbiological contamination situation is discharged detected gas outside the bottle finally by exhaust hose 9 and exhaust hose strainer 10.After the fermentation ventilation finishes, re-use sampling pyrosphere 3, light the ethanol on the sampling pyrosphere 3, aseptic technique disconnects air induction hose 6 fast.
Claims (5)
1. sterile culture proofing unit is characterized in that: comprise air induction hose, exhaust hose, air-tight bottle and be socketed on the sampling pyrosphere of air bypass line, air induction hose and exhaust hose are connected in the air-tight bottle, and the detection substratum is housed in the air-tight bottle.
2. a kind of sterile culture proofing unit according to claim 1, it is characterized in that: described exhaust hose position in bottle is not less than air induction hose.
3. a kind of sterile culture proofing unit according to claim 1 and 2, it is characterized in that: described air induction hose is deep at the bottom of the air-tight bottle bottle.
4. a kind of sterile culture proofing unit according to claim 1 is characterized in that: have breach on the described sampling pyrosphere.
5. a kind of sterile culture proofing unit according to claim 1, it is characterized in that: described sampling pyrosphere is provided with groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310154604.7A CN103205359B (en) | 2013-04-28 | 2013-04-28 | Sterile culture detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310154604.7A CN103205359B (en) | 2013-04-28 | 2013-04-28 | Sterile culture detection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103205359A true CN103205359A (en) | 2013-07-17 |
| CN103205359B CN103205359B (en) | 2014-04-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310154604.7A Active CN103205359B (en) | 2013-04-28 | 2013-04-28 | Sterile culture detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103205359B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628289A (en) * | 2019-01-03 | 2019-04-16 | 伊犁川宁生物技术有限公司 | The sampler and method of air miscellaneous bacteria in a kind of detection zymotechnique |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2622193Y (en) * | 2003-05-27 | 2004-06-30 | 华南理工大学 | Portable inoculating fire-loop of biological reactor |
| CN200996016Y (en) * | 2007-01-11 | 2007-12-26 | 河南利华制药有限公司 | Pressurized inoculating pot |
| CN201834921U (en) * | 2010-08-20 | 2011-05-18 | 明博医药技术开发（上海）有限公司 | Inoculation opening for fermentation tank |
| CN202359116U (en) * | 2011-12-01 | 2012-08-01 | 北京大北农科技集团股份有限公司 | Sterilization device for aseptic sampling of fermentation tank |
| CN203212575U (en) * | 2013-04-28 | 2013-09-25 | 博瑞生物医药泰兴市有限公司 | Sterile culture detection device |
-
2013
- 2013-04-28 CN CN201310154604.7A patent/CN103205359B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2622193Y (en) * | 2003-05-27 | 2004-06-30 | 华南理工大学 | Portable inoculating fire-loop of biological reactor |
| CN200996016Y (en) * | 2007-01-11 | 2007-12-26 | 河南利华制药有限公司 | Pressurized inoculating pot |
| CN201834921U (en) * | 2010-08-20 | 2011-05-18 | 明博医药技术开发（上海）有限公司 | Inoculation opening for fermentation tank |
| CN202359116U (en) * | 2011-12-01 | 2012-08-01 | 北京大北农科技集团股份有限公司 | Sterilization device for aseptic sampling of fermentation tank |
| CN203212575U (en) * | 2013-04-28 | 2013-09-25 | 博瑞生物医药泰兴市有限公司 | Sterile culture detection device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628289A (en) * | 2019-01-03 | 2019-04-16 | 伊犁川宁生物技术有限公司 | The sampler and method of air miscellaneous bacteria in a kind of detection zymotechnique |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103205359B (en) | 2014-04-16 |
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