CN202359116U - Sterilization device for aseptic sampling of fermentation tank - Google Patents
Sterilization device for aseptic sampling of fermentation tank Download PDFInfo
- Publication number
- CN202359116U CN202359116U CN2011204939311U CN201120493931U CN202359116U CN 202359116 U CN202359116 U CN 202359116U CN 2011204939311 U CN2011204939311 U CN 2011204939311U CN 201120493931 U CN201120493931 U CN 201120493931U CN 202359116 U CN202359116 U CN 202359116U
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- China
- Prior art keywords
- pyrosphere
- fermentor tank
- sampling
- sterile sampling
- union lever
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a sterilization device for aseptic sampling of a fermentation tank. The device comprises a fixing clip, a connecting rod and a fire ring, wherein one end of the connecting rod is connected to a sampling tube of the fermentation tank through the fixing clip, and the other end of the connecting rod is connected with the fire ring; and the fire ring is used for ignition and sterilization. The fire ring is fixed on the sampling tube of the fermentation tank and is used for ignition and sterilization, so that the requirement of aseptic environments of the sampling is met, and exogenous bacteria can be furthest prevented from being brought into the fermentation tank from a sampling port. Moreover, the device can be arranged on a stainless steel bioreactor of any size, works safely and reliably, is convenient to operate, and can quickly extinguish the flame, and the length can be adjusted according to requirements; and by the device, the work can be independently and quickly finished by one person.
Description
Technical field
The utility model belongs to the sterile sampling field, particularly relates to a kind of fermentor tank sterile sampling sterilizing unit.
Background technology
In the biological products industry field; One of production unit that it is main is exactly a fermentor tank; Produce used starting material fully under the situation of bacterium; Its most of the time all is in fermentor tank, to accomplish by the temperature and pressure parameter fermentation of formulating, and for the condition of production in the monitor fermentation jar in real time, gets a certain amount of fermented liquid in the fermentor tank at regular intervals between just having to and comes out to carry out analytical test.
Present sampling unit mainly is made up of three valves, and these three valves are connected successively, because employed valve is more, just inevitably has the dead angle, has bacterium to be brought in the fermentor tank, influences the quality of fermenting and then influencing product.The fermenting process monitoring must obtain the fermented liquid of no external source bacterium; Just need utilize pyrosphere to come non-productive operation in the sampling process; The operation of simple and easy pyrosphere commonly used just requires two people or above assistance operation; Wherein a people is responsible for operating pyrosphere, and another people kind container of being responsible for taking is taken a sample, and this has increased the time and the manpower of sampling virtually.
Summary of the invention
The technical problem that (one) will solve
The technical problem that the utility model will solve is: just need utilize pyrosphere to come non-productive operation in the sampling process, require two people or above assistance operation, increase the time and the manpower of sampling.
(2) technical scheme
In order to solve the problems of the technologies described above, the utility model provides a kind of fermentor tank sterile sampling sterilizing unit.
Wherein, said device comprises retaining clip, union lever and pyrosphere, and said union lever one end is connected to through retaining clip on the stopple coupon of fermentor tank, and its other end is connected with pyrosphere, and said pyrosphere is used to the sterilization of lighting a fire.
Further, said retaining clip is provided with set screw, through the openings of sizes of said screw adjustment retaining clip.
Further, said union lever is an expansion link.
Further, said union lever is a stainless steel.
Further, said union lever is the L type.
Further, said device also comprises the fire extinguishing lid, and said fire extinguishing lid is connected on the said pyrosphere.
Further, said fire extinguishing lid is complementary with said pyrosphere.
Further, said fire extinguishing lid flexibly connects with said pyrosphere.
Further, said pyrosphere includes the inside groove that is used to add liquid.
Further, said pyrosphere is a stainless steel.
(3) beneficial effect
Technique scheme has following advantage: through pyrosphere being fixed on the stopple coupon of fermentor tank; Pyrosphere is used to the sterilization of lighting a fire; Thereby satisfied the gnotobasis requirement of sampling, can avoid the external source bacterium to take place to greatest extent from the situation that thief hole is brought into fermentor tank.And this device can be installed on the stainless steel bio-reactor of any size, and safe and reliable, handled easily can be regulated length as required, and can realize quick knock down the flame, and a people is independent to accomplish fast.
Description of drawings
Fig. 1 is the structural representation that a kind of embodiment of the utility model is connected to fermentor tank;
Fig. 2 is the partial enlarged drawing of Fig. 1.
Wherein, 1: retaining clip; 2: union lever; 3: pyrosphere; 4: the fire extinguishing lid; 5: fermentor tank; A1: first sampling valve; A2: second sampling valve; A3: the 3rd sampling valve.
Embodiment
Below in conjunction with accompanying drawing and embodiment, the embodiment of the utility model is described in further detail.Following examples are used to explain the utility model, but are not used for limiting the scope of the utility model.
As illustrated in fig. 1 and 2; Be a kind of embodiment of the utility model, this device comprises retaining clip 1, union lever 2 and pyrosphere 3, and said union lever 2 one ends are connected on the stopple coupon of fermentor tank 5 through retaining clip 1; Its other end is connected with pyrosphere 3, and said pyrosphere 3 is used to the sterilization of lighting a fire.The utility model is through being fixed to pyrosphere on the stopple coupon of fermentor tank, and pyrosphere is used to the sterilization of lighting a fire, thereby has satisfied the gnotobasis requirement of sampling, can avoid the external source bacterium to take place from the situation that thief hole is brought into fermentor tank to greatest extent.And this device can be installed on the stainless steel bio-reactor of any size, and safe and reliable, handled easily can be regulated length as required, and can realize quick knock down the flame, and a people is independent to accomplish fast.
Consult Fig. 1 and 2 once more; Said retaining clip 1 is provided with set screw; Through the openings of sizes of said screw adjustment retaining clip 1, thereby realized that union lever 2 can be connected with the stopple coupon of random shape, realized that just this device can be applied on the stainless steel bio-reactor of any size.In order to reach the purpose of conveniently extinguishing the flame in the pyrosphere, preferably, this device comprises that also fire extinguishing cover 4, and said fire extinguishing is covered 4 and is connected on the said pyrosphere 3.Aseptic condition for the environment of realizing taking a sample includes the inside groove that is used to add liquid in the pyrosphere 3, in pyrosphere 3 inside grooves, add flammable liquid or soak the wick of flammable liquid, comes sterilization through a burning liquid.More preferably, said fire extinguishing lid 4 is complementary with said pyrosphere 3.Fire extinguishing lid 4 shown in the figure flexibly connects with said pyrosphere 3, and for example axle connects or be hinged, thereby has realized being easy to open the purpose of fire extinguishing lid.Pyrosphere 3 can be taked various suitable materials and structure, and preferably, pyrosphere is a stainless steel.
The union lever 2 of the utility model can be chosen various suitable structures and material, and its material is preferably stainless steel.The shape of union lever 2 can be curve shape or bending shape, union lever 2 has been shown among the figure has been the L type with pyrosphere 3 and links to each other, and pyrosphere is reached the lower end of stopple coupon through this kind shape.Preferably, said union lever is an expansion link, thereby has realized the Telescopic of union lever.The realization of Telescopic can be taked variety of way, and for example union lever top perhaps all is set to spring.
The working process of the utility model is following:
1, the second sampling valve A2 that screws opens the first sampling valve A1 and the 3rd sampling valve A3 on the fermentor tank, and sampling line is carried out online steam sterilizing.
2, retaining clip 1 is connected fixing with the stopple coupon of fermentor tank.
3,, regulate the length of union lever 2, thereby pyrosphere 3 is extended to the lower end of thief hole according to the length of stopple coupon.
4, the fire extinguishing of sliding cover 4 and is left pyrosphere 3, adds flammable liquid or soaks the wick of flammable liquid at pyrosphere 3 inside grooves, revolves little the 3rd sampling valve A3, closes the first sampling valve A1, lights a fire.
5, the sterile sampling bottle is placed in the aseptic area in the pyrosphere 3, open the second sampling valve A2 and the 3rd sampling valve A3, connect appearance.
6, sampling is closed the second sampling valve A2 and the 3rd sampling valve A3 after accomplishing, and the fire extinguishing of sliding rapidly covers 4, and knock down the flame unloads the utility model device from fermentor tank, and whole sampling process finishes.
Can find out by above embodiment; The embodiment of the invention is through being fixed to pyrosphere on the stopple coupon of fermentor tank; Pyrosphere is used to the sterilization of lighting a fire, thereby has satisfied the gnotobasis requirement of sampling, can avoid the external source bacterium to take place from the situation that thief hole is brought into fermentor tank to greatest extent.
The above only is the preferred implementation of the utility model; Should be understood that; For those skilled in the art; Under the prerequisite that does not break away from the utility model know-why, can also make some improvement and replacement, these improvement and replacement also should be regarded as the protection domain of the utility model.
Claims (10)
1. fermentor tank sterile sampling sterilizing unit; It is characterized in that said device comprises retaining clip, union lever and pyrosphere, said union lever one end is connected on the stopple coupon of fermentor tank through retaining clip; Its other end is connected with pyrosphere, and said pyrosphere is used to the sterilization of lighting a fire.
2. fermentor tank sterile sampling sterilizing unit as claimed in claim 1 is characterized in that said retaining clip is provided with set screw, through the openings of sizes of said screw adjustment retaining clip.
3. like claim 1 or 2 described fermentor tank sterile sampling sterilizing units, it is characterized in that said union lever is an expansion link.
4. like claim 1 or 2 described fermentor tank sterile sampling sterilizing units, it is characterized in that said union lever is a stainless steel.
5. like claim 1 or 2 described fermentor tank sterile sampling sterilizing units, it is characterized in that said union lever is the L type.
6. fermentor tank sterile sampling sterilizing unit as claimed in claim 1 is characterized in that, said device also comprises the fire extinguishing lid, and said fire extinguishing lid is connected on the said pyrosphere.
7. fermentor tank sterile sampling sterilizing unit as claimed in claim 6 is characterized in that, said fire extinguishing lid is complementary with said pyrosphere.
8. like claim 6 or 7 described fermentor tank sterile sampling sterilizing units, it is characterized in that said fire extinguishing lid flexibly connects with said pyrosphere.
9. like claim 1 or 2 described fermentor tank sterile sampling sterilizing units, it is characterized in that said pyrosphere includes the inside groove that is used to add liquid.
10. like claim 1 or 2 described fermentor tank sterile sampling sterilizing units, it is characterized in that said pyrosphere is a stainless steel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011204939311U CN202359116U (en) | 2011-12-01 | 2011-12-01 | Sterilization device for aseptic sampling of fermentation tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011204939311U CN202359116U (en) | 2011-12-01 | 2011-12-01 | Sterilization device for aseptic sampling of fermentation tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202359116U true CN202359116U (en) | 2012-08-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011204939311U Expired - Lifetime CN202359116U (en) | 2011-12-01 | 2011-12-01 | Sterilization device for aseptic sampling of fermentation tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202359116U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205359A (en) * | 2013-04-28 | 2013-07-17 | 博瑞生物医药泰兴市有限公司 | Sterile culture detection device |
| CN108531366A (en) * | 2017-04-27 | 2018-09-14 | 安琪酵母（睢县）有限公司 | A kind of yeast emulsion tank containing sampler |
-
2011
- 2011-12-01 CN CN2011204939311U patent/CN202359116U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205359A (en) * | 2013-04-28 | 2013-07-17 | 博瑞生物医药泰兴市有限公司 | Sterile culture detection device |
| CN103205359B (en) * | 2013-04-28 | 2014-04-16 | 博瑞生物医药泰兴市有限公司 | Sterile culture detection device |
| CN108531366A (en) * | 2017-04-27 | 2018-09-14 | 安琪酵母（睢县）有限公司 | A kind of yeast emulsion tank containing sampler |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120801 |
|
| CX01 | Expiry of patent term |