CN219489998U - A sampling device for reusable sterilization of bioreactors - Google Patents

Abstract

The utility model discloses a sampling device used for repeated sterilization of a bioreactor, which comprises a sterilizing component, an air inlet end is connected with an external steam pipeline, and a bioreactor is connected to the sterilizing component; the sampling component, The gas end is connected to the gas outlet of the sterilization component, and the sampling component is also provided with an gas outlet. The sampling component includes a sampling bottle, a three-way valve and an exhaust pipeline. The valve pipe two is connected with the valve one in series on the exhaust pipe; the bottom of the three-way valve is connected with the valve pipe three, and the top of the sampling bottle is provided with a connecting head connected with the valve pipe three; the off-line It is sterilized by means of separate sterilization. When used, it is connected to the sampling port of the bioreactor, which allows the bioreactor to cross-sterilize the sampling pipe of the aseptic sampling device at the same time as one-time sterilization, ensuring the sterility of the sampling pipe and effectively avoiding It reduces the waste of energy and reduces the workload of the staff.

Description

A sampling device for repeated sterilization of bioreactors

technical field

The utility model belongs to the technical field of sampling devices for bioreactors, and in particular relates to a sampling device for repeated sterilization of bioreactors.

Background technique

During the aseptic culture process, it is often necessary to sample the culture fluid in the bioreactor, which generally includes aseptic sampling for aseptic follow-up testing, and routine testing sampling for physical and chemical testing of microorganisms or cell morphology, concentration, and product content. During the cultivation process, sampling is generally carried out at least once a day or several times a day, and the growth status of microorganisms or cells in the bioreactor can be judged by the sterility test of the sample and the analysis of physical and chemical index parameters, which can be used for the next step of cultivating parameter regulation. According to the basis, when performing aseptic sampling and routine testing and sampling, it is required to ensure that the sampling process is strictly sterile, otherwise it will lead to product contamination in the bioreactor and cause irreparable huge economic losses.

The current bioreactor sampling method is to weld a sterile sampling valve on the bottom and side of the bioreactor, and configure a special sampling pipe on the sampling valve for sampling. This sampling method can ensure that the culture solution can be taken out of the bioreactor smoothly It will not pollute the culture medium in the bioreactor, but because there is no aseptic protection for the sampling pipeline, when the sampling pipeline is connected to the sample receiving bottle, the interface is easily exposed to the external environment, so that the bioreactor can be taken out The culture solution of the culture medium is polluted by the microorganisms in the surrounding environment, which brings deviation or misjudgment to the aseptic tracking of the sample. The existing solution to the above is to use a disposable sterile sampler to ensure that the sample taken does not come into contact with the external environment and is free from contamination. Microbial contamination, and can be sterilized online, but the price is expensive and the sterile sampling unit cannot be reused, so the practical application is not high.

Utility model content

The purpose of this utility model is to provide a sampling device for repeated sterilization of bioreactors to solve the problem that the existing aseptic samplers proposed in the above background technology cannot be recycled, and the cost is high and the versatility is low. The problem.

In order to achieve the above purpose, the utility model provides the following technical solutions: a sampling device used for repeated sterilization of bioreactors, comprising:

The sterilizing component, the air inlet end is connected with the external steam pipeline, and the bioreactor is connected to the sterilizing component;

In the sampling component, the air inlet end is connected with the air outlet end of the sterilizing component, and the sampling component is also provided with an air outlet part.

Preferably, the sampling assembly includes a sampling bottle, a three-way valve and an exhaust pipeline, the side of the three-way valve is connected to a valve tube two connected to the exhaust pipeline, and a valve one is connected in series on the exhaust pipeline; The bottom of the three-way valve is connected to the valve tube three, and the top of the sampling bottle is provided with a connector connected to the valve tube three, and the top of the three-way valve is connected to the valve tube one connected with the sterilization assembly, The ends of the first valve tube, the second valve tube and the third valve tube are all provided with clamps.

Preferably, a pressure discharge pipe runs through the top of the connector, the bottom end of the pressure discharge pipe is inside the sampling bottle, and the top of the pressure discharge pipe is outside the connector.

Preferably, the sterilizing assembly includes a steam sterilization pipeline and a sampling pipeline, and the valve tube communicates with the bottom end of the sampling pipeline; the connection between the steam sterilization pipeline and the sampling pipeline is also communicated with a connecting valve, and The feed end of the valve is connected to the bioreactor.

Preferably, a silicone tube is connected to the outside of the top of the pressure discharge pipe, and a disc air filter is connected to the end of the silicone tube.

Preferably, the steam sterilization pipeline and the sampling pipeline are respectively connected in series with a valve 2 and a valve 3.

Preferably, integral stop protrusions are formed on both sides of the bottom of the pressure discharge pipe, and an adjustment groove is opened on the bottom of the pressure discharge pipe.

Compared with the prior art, the beneficial effects of the utility model are:

1. It is sterilized by off-line separate sterilization. When used, it is connected to the sampling port of the bioreactor, which allows the bioreactor to sterilize the sampling pipeline of the aseptic sampling device at the same time as one-time sterilization, ensuring that the sampling pipeline is free from contamination. Bacteria, effectively avoiding the waste of energy, while reducing the workload of staff;

2. After each sampling is completed, the sampling pipeline can be purged with high-temperature and high-pressure steam to purge off the residual material liquid attached to the sampling pipeline, keep the sampling pipeline clean for a long time, and avoid pollution in the sampling pipeline , to achieve the purpose of recycling;

3. The designed connector can close the port of the sampling bottle to achieve airtight effect, avoiding external bacteria from entering the sampling bottle, ensuring the aseptic condition in the sampling bottle, and it is easy to install and replace the sampling bottle.

Description of drawings

Fig. 1 is the structural representation of the utility model;

Fig. 2 is the structural representation of the sterilization assembly of the present utility model;

Fig. 3 is a cross-sectional view of the pressure discharge pipeline of the present invention.

In the figure: 1. Sampling bottle; 2. Connecting head; 3. Valve tube 1; 4. Three-way valve; 5. Valve 1; 8, valve tube two; 9, valve tube three; 10, valve two; 11, connecting valve; 12, valve three; 14, steam sterilization pipeline; 15, sampling pipeline.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

Please refer to Fig. 1 to Fig. 3, the utility model provides a technical solution: a sampling device for repeated sterilization of a bioreactor, including,

Sterilization component, the air inlet end is connected to the external steam pipeline, and the whole device is sterilized by connecting pure steam. The sterilization component is connected with a bioreactor. After the entire sampling device is sterilized, the biological reaction The device discharges the internal gas for sampling;

As for the sampling component, the air inlet end is connected with the air outlet end of the sterilizing component, and the sampling component is also provided with an air outlet part for discharging the pre-sterilizing steam.

In this embodiment, preferably, the sampling assembly includes a sampling bottle 1, a three-way valve 4 and an exhaust pipe 6, wherein the sampling bottle 1 is made of high-temperature-resistant transparent glass material, and the design of this material can facilitate observation of the sample when sampling Whether it is clarified, whether there are impurities, and can be repeatedly sterilized, and has a long service life. The side of the three-way valve 4 is connected to the valve pipe 2 8 connected to the exhaust pipe 6, and the valve 1 5 is connected in series on the exhaust pipe 6. Wherein the exhaust pipe 6 is used to discharge the sterilized gas; the bottom of the three-way valve 4 is connected with a valve tube 3 9, and the top of the sampling bottle 1 is provided with a connection connecting with the valve tube 3 9 and sealing the sampling bottle 1 as a whole Head 2. In actual use, sampling bottles 1 of different sizes can be connected by replacing the connecting head 2. When assembling the sampling device, sampling bottles 1 of different sizes are installed according to the sampling requirements, so as to meet different sampling requirements. The connecting head 2 is composed of a bottle cap and a pipeline structure, the bottle cap is connected to the top of the sampling bottle 1, and the top of the three-way valve 4 is connected to a valve tube 1 3 communicating with the sterilization assembly, a valve tube 1 3, a valve tube 2 8, and a valve tube The ends of the three nines are all covered with clamps, which are connected by clamps. It is very convenient to disassemble and clean. Put the sterilized bottle cap on the bottle cap, and then send the sample, and the parts of the sampling device except the sampling bottle can be disassembled for cleaning and maintenance, which improves the utilization efficiency of the sampling device, improves work efficiency, and avoids idle waste.

In this embodiment, preferably, the top of the connector 2 is also pierced with a pressure discharge pipe 7 , the bottom of the pressure discharge pipe 7 is inside the sampling bottle 1 , and the top of the pressure discharge pipe 7 is outside the connector 2 .

In this embodiment, preferably, the sterilizing assembly includes a steam sterilization pipeline 14 and a sampling pipeline 15, and the valve pipe one 3 communicates with the bottom end of the sampling pipeline 15; the connection between the steam sterilization pipeline 14 and the sampling pipeline 15 is also communicated with A valve 11 is connected, and the feed end of the connection valve 11 is connected with the bioreactor.

In this embodiment, preferably, a silicone tube with a length of 10ml is connected to the outside of the top of the pressure relief pipe 7, and the end of the silicone tube is also connected to a disc air filter, which can achieve the effect of filtering air and effectively avoid The external air pollutes the sampling bottle, which ensures the sterility of the sampling bottle and prevents the backlog of the sampling bottle, so that the sampling work can be successfully completed, and the safety of the operator can be effectively guaranteed.

In this embodiment, preferably, a valve two 10 and a valve three 12 are connected in series on the steam sterilization pipeline 14 and the sampling pipeline 15 respectively.

In this embodiment, integrated stop protrusions 72 are formed on both sides of the bottom of the pressure discharge pipe 7, which can realize the limit of the pressure discharge pipe 7, and an adjustment groove 71 is also opened at the bottom of the pressure discharge pipe 7. When the pressure-discharging pipe 7 is inserted into the bottle cap, it can be deformed and compressed through the adjustment groove 71 , and after installation, the position can be fixed by rebounding and cooperating with the limiting protrusion 72 .

Concrete operation steps of the present utility model are as follows:

The first step is to assemble the sampling device: before assembling, clean all parts of the device, including checking whether the gaskets of the three-way valve 4 and valve 1 are intact, and then assemble the sampling device. When assembling the sampling device, you can choose different sizes according to the sampling requirements. Sampling bottle 1, then valve pipe 1 3, valve pipe 2 8, and valve pipe 3 9 are respectively connected with sampling pipe 15, exhaust pipe 6 and connector 2 with clamps, as shown in Figure 1 after assembly;

In the second step, the assembled sampling device is connected to a silicone hose with a length of about 10ml at the pressure discharge pipe 7, and the silicone hose is connected to a disc-type air filter, and the pressure-holding test is carried out on the sampling device. Close the three-way valve 4, manually pressurize the sampling device with a pressure-holding ball, wait for sterilization after the pressure-holding test is normal;

The third step is to place the normal high-pressure sampling device for the pressure-holding test in a steam sterilizer or a high-pressure steam sterilizer for high-pressure steam sterilization, and cool it down for use after the sterilization is completed;

In the fourth step, the valve tube one 3 of the sampling device is connected to the sampling pipeline 15, and then the steam sterilization pipeline 14 and the sampling pipeline 15 are kept under pressure, and the valve two 10 and the valve three 12 are opened during pressure maintenance, and the steam sterilization pipeline 14 pressurize to the upper end of the three-way valve 4, the three-way valve 4, the valve one 5, and the connecting valve 11 are closed;

The fifth step, when sterilizing, while the bioreactor is sterilizing, the three-way valve 4 and the connecting valve 11 are closed, and the valve 2 10, valve 3 12, and valve 1 5 are opened in sequence, and the pure steam is sterilized by steam in sequence Pipeline 14, sampling pipe 15 and exhaust pipe 6 are used to sterilize the inside of the pipeline and the valves installed. After the sterilization is completed, first blow the pipeline and valves to cool with clean air pressure, and then close valves 1, 5 and 3 in sequence. 12. Valve two 10, keep the pressure for use;

The sixth step, when sampling, first pressurize the bioreactor to 0.08-0.12MPa, then open the three-way valve 4 and valve three 12 in sequence, and pass the exhaust pipe 6, silicone hose and respirator from the sampling bottle 1 Drain the residual pressure in the sampling pipeline 15, then slowly open the connecting valve 11 on the bioreactor, and take a sample;

Step 7: After sampling, close the connection valve 11 and the three-way valve 4 in sequence, and then use pure steam to purge the sampling pipeline 15. When purging, open valve 2 10, valve 3 12, and valve 1 5, and the pure steam passes through the steam The sterilization pipeline 14 and the sampling pipeline 15 are discharged from the exhaust pipeline 6, and the residual material liquid in the sampling pipeline 15 is purged out, and finally the sampling device is removed;

The eighth step, the dismantled sampling device, in the biosafety cabinet, disassemble the connector 2 under aseptic conditions, cover the sampling bottle 1 with a sterilized bottle cap, and then send the sample;

In the ninth step, the parts of the sampling device other than the sampling bottle 1 are disassembled for cleaning and maintenance, so as to be used next time.

Although the embodiments of the present invention have been shown and described (see the detailed description above), those of ordinary skill in the art can understand that these can be modified without departing from the principle and spirit of the present invention. The embodiments undergo various changes, modifications, substitutions and variations, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims (7)

1. A sampling device for repeated sterilization of bioreactors, characterized in that: comprising, The sterilizing component, the air inlet end is connected with the external steam pipeline, and the bioreactor is connected to the sterilizing component; In the sampling component, the air inlet end is connected with the air outlet end of the sterilizing component, and the sampling component is also provided with an air outlet part. 2. A kind of sampling device for bioreactor reusable sterilization according to claim 1, characterized in that: said sampling assembly includes a sampling bottle (1), a three-way valve (4) and an exhaust pipe (6), the side of the three-way valve (4) is connected with a valve tube two (8) connected to the exhaust pipe (6), and a valve one (5) is connected in series on the exhaust pipe (6); The bottom of the three-way valve (4) is connected with a valve tube three (9), and the top of the sampling bottle (1) is provided with a connector (2) connected to the valve tube three (9), and the three-way The top of the valve (4) is connected with a valve pipe one (3) connected with the sterilization assembly, and the ends of the valve pipe one (3), valve pipe two (8) and valve pipe three (9) are all sleeved with clamp. 3. A sampling device for bioreactor reusable sterilization according to claim 2, characterized in that: the top of the connector (2) also runs through a pressure relief pipe (7), the The bottom end of the pressure discharge pipe (7) is inside the sampling bottle (1), while the top end of the pressure discharge pipe (7) is outside the connector (2). 4. a kind of sampling device that is used for bioreactor reusable sterilization according to claim 2, is characterized in that: described sterilizing component comprises steam sterilization pipeline (14) and sampling pipeline (15), so Described valve pipe one (3) is communicated with the bottom of sampling pipeline (15); Described steam sterilizing pipeline (14) is also communicated with connecting valve (11) with the junction of sampling pipeline (15), and this connecting valve (11 ) The feed end is connected with the bioreactor. 5. A kind of sampling device for bioreactor reusable sterilization according to claim 3, it is characterized in that: the top outside of the pressure discharge pipe (7) is also connected with a silicone tube, the silicone tube A dish air filter is also attached at the end. 6. A sampling device for repeated sterilization of bioreactors according to claim 4, characterized in that: the steam sterilization pipeline (14) and the sampling pipeline (15) are respectively connected in series with two valves (10) and valve three (12). 7. A sampling device for re-sterilizable bioreactors according to claim 3, characterized in that: integrated stop protrusions are formed on both sides of the bottom of the pressure discharge pipe (7) (72), an adjustment groove (71) is also opened at the bottom of the pressure discharge pipe (7).