CN215103245U - Flameless fermentation inoculation device - Google Patents
Flameless fermentation inoculation device Download PDFInfo
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- CN215103245U CN215103245U CN202022726930.9U CN202022726930U CN215103245U CN 215103245 U CN215103245 U CN 215103245U CN 202022726930 U CN202022726930 U CN 202022726930U CN 215103245 U CN215103245 U CN 215103245U
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Abstract
The utility model provides a no flame fermentation inoculation mouth device, it relates to biological fermentation equipment field, concretely relates to no flame fermentation inoculation mouth device's improvement. The fermentation tank comprises an insertion mechanism, a base, a fermentation tank body and a connecting pipe, wherein the insertion mechanism is connected with the base; the inserting mechanism comprises a connector, an inoculation port protective sleeve and an inoculation needle, wherein the bottom end of the connector is fixedly connected with the inoculation port protective sleeve, the inoculation needle is fixedly connected in the inoculation port protective sleeve, and the top end of the inoculation needle is fixedly connected with the connector; the base comprises a base, a connector, an electric heating wire and an elastic piston, the connector is arranged on the top surface of the base, the electric heating wire is fixedly connected in an inner cavity of the connector, a through hole is formed in the base and located under the electric heating wire, and the elastic piston is arranged at the bottom end of the through hole. The utility model discloses can be when guaranteeing that the process of transplanting a kind accords with aseptic rule with heating source control in limited explosion-proof space.
Description
Technical Field
The utility model relates to a biological fermentation field, concretely relates to improvement of flameless fermentation inoculation device.
Background
In the biological fermentation process, the fermentation process needs a completely clean production to prevent the environmental contamination of the culture. During industrial tank-loading production, slant or dry bacteria powder preserved at low temperature is first activated and enriched in a shake flask, and after reaching certain concentration, the thallus is transferred to a seeding tank for culture. This stage is usually done in a sterile clean laboratory, then transferred to a seed tank, and then scaled up step by step for large-scale production. The production workshops in which the seed culture tanks and the fermentation tanks are located are not sterile and highly clean workshops generally, and the inoculation process from the shake flask of a clean laboratory to the production fermentation tanks needs to ensure that the production fermentation tanks are not infected with bacteria, and a proper sterile operation method needs to be adopted.
In the prior art, in order to ensure sterility, a flame ring rotating method is generally adopted for seed transformation, namely a metal ring with a groove and a handle is used, alcohol cotton is placed in the groove, and the ring is sleeved in a seed transformation opening and is ignited; when the seeds are transferred, the air flow is reduced, the cover of the seed transfer cover is opened, the exhaust valve is closed, and when the pressure of the tank is reduced to be close to zero, the seed liquid is poured into the tank from flame to complete the seed transfer operation. However, there are some problems, such as uneven flame height, even flame extinction, and flying of alcohol cotton due to production, installation failure, or improper operation of alcohol cotton, etc. often occur during the operation, and secondly, open flame cannot be used within 150m in class a workshops or workshops containing class a operation units, which makes it impossible to use the flame ring inoculation method on class a fire-proof-grade fermentation process lines using ethanol or ethyl acetate extraction processes in the post-fermentation treatment processes, or to separately install separate fermentation and separation workshops in a workshop independent of fermentation.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an it is not enough to prior art, provide one kind and avoid the naked light to use, and reduce a flameless fermentation inoculation device of the risk of workshop deflagration.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the fermentation tank comprises an insertion mechanism 1, a base 2, a fermentation tank body 3 and a connecting pipe 4, wherein the insertion mechanism 1 is connected with the base 2, the base 2 is fixedly connected to the fermentation tank body 3, and the top end of the insertion mechanism 1 is connected with the connecting pipe 4;
the inserting mechanism 1 comprises a connector 11, an inoculation port protective sleeve 12 and an inoculation needle 13, wherein the bottom end of the connector 11 is fixedly connected with the inoculation port protective sleeve 12, the inoculation needle 13 is fixedly connected in the inoculation port protective sleeve 12, and the top end of the inoculation needle 13 is fixedly connected with the connector 11;
Further, the inoculation port protective sheath 12 and the interface 22 are threadably connected. The threaded connection mode is simple to mount and dismount and has good tightness.
Further, springs 241 are provided at both sides of the elastic piston 24. The spring 241 pulls the elastic plunger 24 back to the original position by the elastic force when the inoculating needle 13 is withdrawn, thereby closing the through hole 211.
Further, the inoculating needle 13 is a cylindrical stainless steel needle member. The stainless steel inoculating needle 13 is corrosion resistant and high temperature resistant, and has a longer service life.
The utility model discloses a theory of operation: insert its base 2 that is located fermentation cylinder body 3 of the utility model into the insertion mechanism 1, carry out threaded connection through the internal thread of inoculation port protective sheath 12 and the external screw thread of interface 22 at this moment, fix insertion mechanism 1 on base 2, in this process inoculating needle 13 passes from electric heat heating wire 23 center, and pass through-hole 211, push open elastic piston 24 simultaneously, get into the cavity of fermentation cylinder body 3, open the power-on switch of electric heat heating wire 23 at this moment, form a confined cavity between inoculation port protective cap 12 and base 21 and carry out high temperature heating, through the thermal conductance effect, make whole inoculating needle 13 and confined cavity all heated the disinfection, the thalli of carrying the cultivation to inoculating needle 13 through connecting pipe 4 disinfected in advance after the disinfection is accomplished, and then the cultivation is carried to in the fermentation cylinder body 3; when the insertion mechanism 1 is pulled out from the base 2, the elastic piston 24 will automatically return to the original position by the elastic force of the spring 241, blocking the through hole 211, so that the inner cavity of the fermenter tank 3 is isolated from the outside.
After the technical scheme is adopted, the utility model discloses beneficial effect does: through set up electric heat heater strip 23 in the cavity that forms after inoculation mouth protective sheath 12 and interface 22 carry out threaded connection, electric heat heater strip 23 circular telegram carries out high temperature sterilization to inoculating needle 13 and whole cavity, can accomplish the sterilization and disinfection to inoculating needle 13 part like this, and the guarantee is moved kind of process and is accorded with aseptic rule, controls the heating source in limited explosion-proof space simultaneously, prevents the class A workshop detonation condition because of the heat source causes.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.
Fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a schematic view of the working state of the present invention.
Description of reference numerals: the inoculation device comprises an insertion mechanism 1, a connector 11, an inoculation port protective sleeve 12, an inoculation needle 13, a base 2, a base 21, a through hole 211, a port 22, an electric heating wire 23, an elastic piston 24, a spring 241, a fermentation tank body 3 and a connecting pipe 4.
Detailed Description
Referring to fig. 1 to 2, the technical solution adopted by the present embodiment is: a flameless fermentation inoculation port device comprises an insertion mechanism 1, a base 2, a fermentation tank body 3 and a connecting pipe 4, wherein the insertion mechanism 1 is connected with the base 2, the base 2 is fixedly connected to the fermentation tank body 3, and the top end of the insertion mechanism 1 is connected with the connecting pipe 4; the inserting mechanism 1 comprises a connector 11, an inoculation port protective sleeve 12 and an inoculation needle 13, wherein the bottom end of the connector 11 is fixedly connected with the inoculation port protective sleeve 12, the inoculation needle 13 is fixedly connected in the inoculation port protective sleeve 12, the top end of the inoculation needle 13 is fixedly connected with the connector 11, and the connector 11 can be connected with a bottle or an injector filled with bacteria liquid through a connecting pipe 4 made of silica gel or other materials; base 2 contains base 21, interface 22, electric heat heating wire 23, elastic piston 24, base 21 direct mount is on the fermentation cylinder jar body 3, and the base 21 top surface is equipped with interface 22, and interface 22's outside size and shape and the inboard size of the inoculation mouth protective cap 12 in the insertion mechanism 1 cooperate completely, fixedly connected with electric heat heating wire 23 in interface 22's the inner chamber, be equipped with through-hole 211 in the base 21, through-hole 211 is located electric heat heating wire 23 under, and the bottom of through-hole 211 is equipped with elastic piston 24, sets up through-hole 211 in the base 21 and makes the outside and the inner chamber intercommunication of the fermentation cylinder jar body 3.
Preferably, the inoculation port protective sleeve 12 and the interface 22 are in threaded connection, and the threaded connection is convenient to install and disassemble and has good tightness.
Preferably, springs 241 are arranged on two sides of the elastic piston 24, and the springs 241 pull the elastic piston 24 back to the original position by the elastic force when the inoculating needle 13 is pulled out, so as to close the through hole 211.
Preferably, the inoculating needle (13) is a cylindrical stainless steel needle component, the stainless steel has good corrosion resistance and heat resistance, the stainless steel can still maintain the excellent physical and mechanical properties at high temperature, and the service life of the inoculating needle (13) is longer.
The utility model discloses a theory of operation: insert its base 2 that is located fermentation cylinder body 3 of the utility model into the insertion mechanism 1, carry out threaded connection through the internal thread of inoculation port protective sheath 12 and the external screw thread of interface 22 at this moment, fix insertion mechanism 1 on base 2, in this process inoculating needle 13 passes from electric heat heating wire 23 center, and pass through-hole 211, push open elastic piston 24 simultaneously, get into the cavity of fermentation cylinder body 3, open the power-on switch of electric heat heating wire 23 at this moment, form a confined cavity between inoculation port protective cap 12 and base 21 and carry out high temperature heating, through the thermal conductance effect, make whole inoculating needle 13 and confined cavity all heated the disinfection, the thalli of carrying the cultivation to inoculating needle 13 through connecting pipe 4 disinfected in advance after the disinfection is accomplished, and then the cultivation is carried to in the fermentation cylinder body 3; when the insertion mechanism 1 is pulled out from the base 2, the elastic piston 24 will automatically return to the original position by the elastic force of the spring 241, blocking the through hole 211, so that the inner cavity of the fermenter tank 3 is isolated from the outside.
The above description is only for the purpose of illustrating the technical solutions of the present invention and not for the purpose of limiting the same, and other modifications or equivalent replacements made by those of ordinary skill in the art to the technical solutions of the present invention should be covered within the scope of the claims of the present invention as long as they do not depart from the spirit and scope of the technical solutions of the present invention.
Claims (4)
1. A flameless fermentation inoculation device is characterized in that: the fermentation tank comprises an insertion mechanism (1), a base (2), a fermentation tank body (3) and a connecting pipe (4), wherein the insertion mechanism (1) is connected with the base (2), the base (2) is fixedly connected to the fermentation tank body (3), and the top end of the insertion mechanism (1) is connected with the connecting pipe (4);
the inserting mechanism (1) comprises a connector (11), an inoculation port protective sleeve (12) and an inoculation needle (13), wherein the bottom end of the connector (11) is fixedly connected with the inoculation port protective sleeve (12), the inside of the inoculation port protective sleeve (12) is fixedly connected with the inoculation needle (13), and the top end of the inoculation needle (13) is fixedly connected with the connector (11);
base (2) contain base (21), interface (22), electric heat heater strip (23), elastic piston (24), base (21) top surface is equipped with interface (22), fixedly connected with electric heat heater strip (23) in the inner chamber of interface (22), be equipped with through-hole (211) in base (21), through-hole (211) are located electric heat heater strip (23) under, and the bottom of through-hole (211) is equipped with elastic piston (24).
2. The flameless fermentation inoculation port device of claim 1, wherein: the inoculation port protective sleeve (12) is in threaded connection with the interface (22).
3. The flameless fermentation inoculation port device of claim 1, wherein: and springs (241) are arranged on two sides of the elastic piston (24).
4. The flameless fermentation inoculation port device of claim 1, wherein: the inoculating needle (13) is a cylindrical stainless steel needle component.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202022726930.9U CN215103245U (en) | 2020-11-23 | 2020-11-23 | Flameless fermentation inoculation device |
Applications Claiming Priority (1)
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CN202022726930.9U CN215103245U (en) | 2020-11-23 | 2020-11-23 | Flameless fermentation inoculation device |
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CN215103245U true CN215103245U (en) | 2021-12-10 |
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CN202022726930.9U Active CN215103245U (en) | 2020-11-23 | 2020-11-23 | Flameless fermentation inoculation device |
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