CN210030686U - Device for sterilizing inoculation port of fermentation tank and fermentation system - Google Patents
Device for sterilizing inoculation port of fermentation tank and fermentation system Download PDFInfo
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- CN210030686U CN210030686U CN201920653424.6U CN201920653424U CN210030686U CN 210030686 U CN210030686 U CN 210030686U CN 201920653424 U CN201920653424 U CN 201920653424U CN 210030686 U CN210030686 U CN 210030686U
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Abstract
The utility model provides a device and fermentation system for being directed at fermentation cylinder inoculation mouth carries out sterilization relates to sterilization technical field. The device for sterilizing comprises a hollow shell and a gas pipeline, wherein the gas pipeline comprises a flame spraying pipeline and a gas transmission pipeline, the flame spraying pipeline is enclosed into a hollow column shape, the gas transmission pipeline is used for connecting a gas source with the flame spraying pipeline, the flame spraying pipeline is located in an inner cavity of the hollow shell, and a plurality of flame nozzles are arranged on the flame spraying pipeline. The fermentation system comprises the device, and when the device is used, the device is arranged at the inoculation port, so that the covering area of flame generated by the sterilization device is large, the concentration is high, and quick and effective sterilization can be realized.
Description
Technical Field
The utility model relates to a sterilization technical field particularly, relates to a device and fermentation system for being used for disinfecting to fermentation cylinder inoculation mouth.
Background
The fermentation tank is a device used for culturing microorganisms in industry, and is commonly used for culturing microbial biomass and secondary metabolites thereof in the fields of biological medicine and the like. In the use process of the fermentation tank, all pipelines, the tank body and the culture medium need to be disinfected firstly, and then the cultured strains are inoculated into the fermentation tank. In order to avoid contamination of mixed bacteria, the inoculation of the strain needs to be carried out in a strict sterile environment.
The common method mainly adopts a flame inoculation method, namely, an annular groove is arranged around an inoculation port, absorbent cotton and alcohol are placed in the groove, the inoculation port is ignited before the inoculation port is opened for sterilization, the inoculation port is opened after the sterilization is finished, strains are inoculated in a fermentation tank body, an inoculation port cover is covered after the inoculation is finished, and the whole process needs to ensure that the flame around the inoculation port cannot be extinguished. The current common improved methods mainly use an annular alcohol lamp for inoculation operation, use a piercing method for inoculation by covering a rubber pad on an inoculation port, use a peristaltic pump and the like for inoculation by connecting a plurality of pipelines, and the like.
However, the existing sterilization method has the problems of incomplete sterilization, low sterilization efficiency and the like, and the flame intensity in the sterilization and inoculation processes is not easy to control.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a device for being directed at fermentation cylinder inoculation mouth carries out the sterilization, aims at improving sterilization efficiency to make the sterilization process more thorough.
Another object of the present invention is to provide a fermentation system, which can perform the sterilization work quickly and effectively before and after the inoculation.
The utility model discloses a realize like this:
the utility model provides a device for being directed at fermentation cylinder inoculation mouth carries out sterilization, includes cavity casing and gas pipeline, and the gas pipeline is including enclosing into the flame projecting pipeline of cavity column and the gas transmission pipeline that is used for connecting air supply and flame projecting pipeline, and the flame projecting pipeline is arranged in the inner chamber of cavity casing, is provided with a plurality of flame spouts on the flame projecting pipeline, and a plurality of flame spouts distribute in the circumference and the axial of flame projecting pipeline.
Further, in the preferred embodiment of the present invention, the flaming pipeline is spiral, a plurality of flame nozzles are arranged upwards along the circumferential direction on the top pipeline of the flaming pipeline, and a plurality of flame nozzles facing the center are arranged along the circumferential direction on the pipeline below the top pipeline of the flaming pipeline.
Further, in a preferred embodiment of the present invention, a bottom of the hollow housing is provided with a connecting port for fixing the hollow housing to the inoculation port of the fermentation tank.
Further, in the preferred embodiment of the present invention, the gas pipeline is provided with a regulating valve for regulating the gas amount, and the gas outlet end of the gas pipeline is provided with an igniter.
Further, in the preferred embodiment of the present invention, the gas cylinder further comprises a rubber hose, one end of the rubber hose is communicated with the gas inlet end of the gas pipeline, and the other end of the rubber hose is provided with a connecting portion for connecting with the gas cylinder.
Further, in the preferred embodiment of the present invention, a grip handle is installed on the outer wall of the hollow casing, and a heat insulating sleeve is sleeved on the grip handle.
Further, in the preferred embodiment of the present invention, a high temperature resistant coating is disposed on both the inner wall and the outer wall of the hollow shell.
Further, in the preferred embodiment of the present invention, a support frame for supporting the device on the fermentation tank is further provided on the hollow casing.
A fermentation system comprises a fermentation tank and the device for sterilizing the inoculation port of the fermentation tank, wherein the device is arranged at the inoculation port of the fermentation tank.
Further, in the preferred embodiment of the present invention, the air-conditioning system further comprises an air source, wherein the air source is communicated with the air inlet end of the air pipeline.
The utility model has the advantages that: the utility model discloses a device that is used for disinfecting fermentation cylinder inoculation mouth that above-mentioned design obtained, it is through inputing the gas from being located the outside gas pipeline of cavity casing, through being located the inside gas pipeline of cavity casing, from a plurality of flame spout blowout on the gas pipeline, the degree of consistency of flame is higher after igniteing, and the cover face is bigger, is showing the sterilization rate that improves and makes the sterilization more thorough.
The utility model also provides a fermentation system, including fermentation cylinder and the above-mentioned device that is used for disinfecting fermentation cylinder inoculation mouth, install sterilization apparatus in inoculation mouth department during the use, the coverage area of the flame that sterilization apparatus produced is great, intensive height, can realize quick effectual sterilization.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a view showing a state of use of a sterilization apparatus;
FIG. 2 is a schematic view of the gas duct of FIG. 1 inside the housing;
FIG. 3 is an enlarged view of region IV of FIG. 2.
Icon: 100-a sterilization device; 110-a hollow shell; 111-a flame outlet; 112-an interface section; 120-a gas pipeline; 121-flame-throwing pipe; 122-flame ports; 123-gas transmission pipeline; 124-regulating valve; 130-rubber hose; 132-a connecting portion; 140-a support frame; 142-a support bar; 143-top struts; 144-bottom struts.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "length", "width", "thickness", "upper", "lower", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, are not to be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
Referring to fig. 1, an embodiment of the present invention provides a sterilization apparatus 100 (i.e., an apparatus for sterilizing a fermentor inoculation port), which includes a hollow housing 110 and a gas pipeline 120. The hollow casing 110 serves to protect the generated flame from environmental changes, and the gas pipe 120 serves to generate a flame for sterilization. The traditional inoculation port sterilization device is an alcohol flame parallel or vertical to the inoculation port, the flame is easily influenced by the environment, the sterilization is not thorough, and the pollution is easily caused in the inoculation process.
Specifically, the gas pipeline 120 includes a flame spraying pipeline 121 enclosing a hollow column shape and a gas transmission pipeline 123 for connecting a gas source with the flame spraying pipeline 121, the flame spraying pipeline 121 is located in an inner cavity of the hollow housing 110, a plurality of flame nozzles 122 are arranged on the flame spraying pipeline 121, and the plurality of flame nozzles 122 are distributed in the circumferential direction and the axial direction of the flame spraying pipeline 121.
In the preferred embodiment of the present invention, the flaming pipe 121 is spiral, a plurality of flame nozzles 122 are arranged upward along the circumferential direction on the top pipe of the flaming pipe 121, and a plurality of flame nozzles 122 are arranged toward the center along the circumferential direction on the pipe below the top pipe of the flaming pipe 121, so as to form an overall upward flame. The whole pipeline (including the top) of the rotary gas pipeline 120 is provided with the flame nozzle 122, so that the sterilization area is further enhanced, and the sterilization is thorough.
Referring to fig. 1-3, the top and the bottom of the hollow casing 110 are provided with flame outlets 111, the gas transmission pipe 123 transmits gas to the flame spraying pipe 121 inside the hollow casing 110, after ignition, the flame spraying ports 122 can generate flames continuously, the coverage of the flames is larger, the concentration is higher, and the sterilization is quick and effective.
It should be noted that the type of gas transported in the gas pipeline 120 is not limited, and may be common natural gas or the like. Preferably, the gas pipe 120 may have a plurality of extending tendencies inside the hollow shell 110, and may be in a spiral rising form, so as to increase the length extending inside the hollow shell 110, thereby achieving the purpose of making the flame concentration higher.
It is to be added that the traditional inoculation port and the tank body are integrated, and the alcohol flame groove is very easy to become a potential pollution source. The utility model relates to an external sterilization device 100, which can not affect the fermentation of the tank body and the strain.
Further, a regulating valve 124 for regulating the amount of gas is disposed on the gas transmission pipe 123 outside the hollow shell 110, and an igniter is disposed at the gas outlet end of the gas transmission pipe 123. The regulating valve 124 can effectively regulate the gas flow in the gas pipeline 120, thereby well controlling the flame height. In the practical application in-process, all the flame height that needs is higher around the inoculation, and need small flame during the inoculation can, adopt the utility model discloses the sterilization apparatus 100 that provides can adapt to the flame requirement in different stages better, accurate control flame height.
Further, the sterilization apparatus 100 further comprises a rubber hose 130, one end of the rubber hose 130 is communicated with the air inlet end of the air transmission pipeline 123, and the other end of the rubber hose 130 is connected with a connecting portion 132 for connecting with a gas tank. The length of the rubber hose 130 can be selected as required, and the connecting portion 132 at the end portion thereof is preferably detachably connected, so that it can be adapted to gas cartridges of different models.
Further, the hollow casing 110 is cylindrical, the top of the hollow casing 110 is open, and the bottom of the hollow casing 110 is provided with a mouthpiece 112 for fixing the hollow casing 110 to the seed port of the fermentation tank. The outer diameter of the interface portion 112 is smaller than the outer diameter of the hollow housing 110, and the top outer diameter of the interface portion 112 is larger than the bottom outer diameter. During inoculation, seed liquid is fed from the top opening of the hollow shell 110 and is output from the interface part 112 at the bottom of the hollow shell 110, and the funnel-shaped shape of the interface part 112 can be clamped with the outer side of the inoculation opening of the fermentation tank, so that strains can smoothly enter the tank body.
Further, a holding handle is mounted on the outer wall of the hollow housing 110, the shape of the holding handle is not limited, and an accommodating gap for accommodating a human hand is formed between the holding handle and the outer wall of the hollow housing 110, so that an operator can transfer the position of the sterilization apparatus 100 more conveniently as required.
In the preferred embodiment of the present invention, a heat insulation sleeve (not shown) is sleeved on the grip handle. The material of heat insulating sleeve is not limited, can be the rubber material in some embodiments, overlaps and establishes on gripping the handle, is difficult to be scalded by high temperature when making operating personnel grip, improves the factor of safety of operation.
In the preferred embodiment of the present invention, the inner wall and the outer wall of the hollow housing 110 are coated with high temperature resistant coatings, the hollow housing 110 itself can be a steel plate, and in order to adapt to the high temperature operation environment, the two surfaces of the steel plate can be coated with high temperature resistant coatings, so as to increase the service life of the device.
It should be noted that the high temperature resistant coating is formed by coating a commercially available high temperature resistant coating, which is an existing material, and the specific components are not described in detail.
In the preferred embodiment of the present invention, the bottom of the hollow housing 110 is provided with a support 140 for supporting the device on the fermentation tank. The supporting frame 140 comprises at least two supporting rods 142, each supporting rod 142 comprises a top supporting rod 143 and a bottom supporting rod 144, the top of the top supporting rod 143 is connected with the hollow shell 110, and the bottom of the top supporting rod 143 is connected with the top of the bottom supporting rod 144; the spacing between the bottom struts 144 is compatible with the size of the mounting platform. Generally, the bottom struts 144 extend in a vertical direction and the top struts 143 are used to connect the bottom struts 144 with the edges of the hollow shell 110.
It should be noted that the number of the supporting rods 142 is set as required, and may be two, three or more. When the sterilization apparatus 100 is installed at the inoculation port of the fermentation tank, please refer to fig. 2, it is only necessary to place the bottom support rod 144 around the inoculation port of the fermentation tank, which increases the stability of the operation of the whole apparatus.
The embodiment of the utility model provides a fermentation system is still provided, including fermentation cylinder and the above-mentioned device that is used for disinfecting to the fermentation cylinder inoculation mouth, the device sets up the inoculation mouth department at the fermentation cylinder. The whole fermentation system has high operation stability, can obviously improve the flame intensity and can quickly and effectively sterilize.
In the preferred embodiment of the present invention, the present invention further comprises an air source, the air source is communicated with the air inlet end of the air pipeline 123. The gas pipeline 123 is supplied with gas through a gas source, which can be in the form of a common gas tank, and the specific model of the gas tank is not limited.
The method of using the sterilization apparatus 100 will be specifically described below. The device is placed above the inoculation opening of the fermentation tank, and the lower interface part 112 of the device is clamped outside the inoculation opening of the fermentation tank. The outer rubber hose 130 of the gas pipeline 120 is connected with a combustible gas tank, a gas control valve is opened slightly, and a fire source is adopted to ignite the inner side of the device and the top flame nozzle 122. Adjusting the regulating valve 124, and continuously sterilizing the environment around the inoculation port for 2-3 minutes after the flame is stabilized. After the sterilization is finished, adjusting a gas control valve, reducing the flame height, opening the inoculation port upper cover by using a straight barrel wrench, inoculating the seed liquid into the fermentation tank, and covering the fermentation tank inoculation port cover. Adjusting the adjusting valve 124 after inoculation is finished, and continuing heating and sterilizing for 1-2 minutes. And finally, closing the gas valve, extinguishing the flame, naturally cooling for 10-15 minutes, and taking down.
To sum up, the utility model provides a pair of a device for being directed at fermentation cylinder inoculation mouth carries out sterilization, it is through inputing the gas from being located the outside gas pipeline of cavity casing, through being located the inside gas pipeline of cavity casing, from a plurality of flame spout blowout on the gas pipeline, the degree of consistency of flame is higher after igniteing, and the cover face is bigger, is showing the sterilization rate of improvement and makes the sterilization more thorough.
The utility model provides a pair of fermentation system, including gas jar and above-mentioned sterilization apparatus, the gas jar is used for the gas pipeline air feed, installs sterilization apparatus in inoculation mouth department during the use, and the flame intensity that sterilization apparatus produced is high, and the efficiency of sterilization is faster, and the sterilization effect is more ideal.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The device for sterilizing the inoculation port of the fermentation tank is characterized by comprising a hollow shell and a gas pipeline, wherein the gas pipeline comprises a flame spraying pipeline and a gas transmission pipeline, the flame spraying pipeline is enclosed into a hollow column shape, the gas transmission pipeline is used for connecting a gas source with the flame spraying pipeline, the flame spraying pipeline is positioned in an inner cavity of the hollow shell, a plurality of flame nozzles are arranged on the flame spraying pipeline, and the flame nozzles are distributed in the circumferential direction and the axial direction of the flame spraying pipeline.
2. The device of claim 1, wherein the flaming pipeline is spiral, a plurality of upward flame nozzles are circumferentially arranged on the top pipeline of the flaming pipeline, and a plurality of center-oriented flame nozzles are circumferentially arranged on the pipeline below the top pipeline of the flaming pipeline.
3. The apparatus of claim 1, wherein the bottom of the hollow housing is provided with an interface for securing the hollow housing to a fermenter inoculation port.
4. The device as claimed in claim 1, wherein the gas pipeline is provided with a regulating valve for regulating the gas quantity, and an igniter is arranged at the gas outlet end of the gas pipeline.
5. The device as claimed in claim 4, further comprising a rubber hose, one end of the rubber hose is communicated with the air inlet end of the air delivery pipe, and the other end of the rubber hose is provided with a connecting part for connecting with a gas tank.
6. The device of claim 1, wherein a grip handle is mounted on an outer wall of the hollow housing, and a heat insulating sleeve is sleeved on the grip handle.
7. The apparatus of claim 1, wherein a high temperature resistant coating is provided on both the inner and outer walls of the hollow housing.
8. The apparatus of claim 1, wherein a support frame is further provided on the hollow housing for supporting the apparatus on the fermenter.
9. A fermentation system comprising a fermenter and the apparatus of any one of claims 1 to 8, the apparatus being provided at an inoculation port of the fermenter.
10. The system of claim 9, further comprising a gas source in communication with the gas input end of the gas delivery conduit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920653424.6U CN210030686U (en) | 2019-05-08 | 2019-05-08 | Device for sterilizing inoculation port of fermentation tank and fermentation system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920653424.6U CN210030686U (en) | 2019-05-08 | 2019-05-08 | Device for sterilizing inoculation port of fermentation tank and fermentation system |
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| Publication Number | Publication Date |
|---|---|
| CN210030686U true CN210030686U (en) | 2020-02-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920653424.6U Expired - Fee Related CN210030686U (en) | 2019-05-08 | 2019-05-08 | Device for sterilizing inoculation port of fermentation tank and fermentation system |
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| CN (1) | CN210030686U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116349551A (en) * | 2023-03-30 | 2023-06-30 | 黑龙江省绿色食品科学研究院 | Edible fungus liquid strain seed production machine and use method thereof |
-
2019
- 2019-05-08 CN CN201920653424.6U patent/CN210030686U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116349551A (en) * | 2023-03-30 | 2023-06-30 | 黑龙江省绿色食品科学研究院 | Edible fungus liquid strain seed production machine and use method thereof |
| CN116349551B (en) * | 2023-03-30 | 2023-10-10 | 黑龙江省绿色食品科学研究院 | Edible fungus liquid strain seed production machine and use method thereof |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200207 Termination date: 20210508 |
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| CF01 | Termination of patent right due to non-payment of annual fee |