CN217922102U - Anaerobe inoculation transfer bottle - Google Patents

Abstract

The application relates to anaerobe switching technical field discloses an anaerobe inoculation switching bottle, and it includes bottle, bottle lid and oxygen replacement mechanism, the bottleneck has been seted up to the bottle, the bottle lid is used for sealing or opens the bottleneck, oxygen replacement mechanism with the bottle is connected, oxygen replacement mechanism is used for right the bottle supplies nitrogen gas in order to incite somebody to action oxygen replacement in the bottle. This application has the effect that reduces the interior oxygen content of switching bottle, reduces among the switching process anaerobe bacterial loss.

Description

Anaerobe inoculation transfer bottle

Technical Field

The application relates to the field of anaerobic bacterium transfer technology, in particular to an anaerobic bacterium inoculation transfer bottle.

Background

In the production process of biological engineering products such as biological medicines, biochemical products, biological fuels, brewed foods and the like, an anaerobic fermentation system cannot be separated, and anaerobic bacteria are the most important components in the anaerobic fermentation system.

In the actual production process, anaerobic bacteria cultured in a seeding tank need to be transferred into a fermentation tank so that organic substances in the fermentation tank are subjected to anaerobic fermentation and converted into products required by people.

At present, the existing anaerobic bacterium transfer is mostly carried out through an anaerobic bottle, a worker firstly puts anaerobic bacterium strains cultured in a seed tank into the anaerobic bottle through a bottle opening of the anaerobic bottle, then the bottle opening of the anaerobic bottle is sealed, and the anaerobic bottle is used for transferring the strains in the seed tank into a fermentation tank.

In view of the above-mentioned related technologies, the inventor found that when anaerobic bacteria are placed in an anaerobic bottle through a bottle opening of the anaerobic bottle, part of oxygen exists in the anaerobic bottle, and the anaerobic bacteria have low activity when meeting oxygen, and then the defect that the anaerobic bacteria are easy to lose during transfer process exists.

SUMMERY OF THE UTILITY MODEL

In order to alleviate and cause the problem of anaerobe bacterial loss easily in carrying out the switching process to the anaerobe bacterial, this application provides an anaerobe inoculation switching bottle.

The application provides an anaerobe inoculation transfer bottle adopts following technical scheme:

the bottle body is provided with a bottle opening, the bottle cover is used for sealing or opening the bottle opening, the oxygen replacing mechanism is connected with the bottle body, and the oxygen replacing mechanism is used for supplying nitrogen to the bottle body so as to replace the oxygen in the bottle body.

Through adopting above-mentioned technical scheme, set up oxygen replacement mechanism on the bottle, when switching to the anaerobe bacterial, the anaerobe bacterial of staff in the at first dona tub is put into the bottle, then seals the bottleneck with the bottle lid, utilizes oxygen replacement mechanism to let in nitrogen gas in to the bottle then, utilizes the nitrogen gas that lets in to replace the oxygen in the bottle to reduce the content of oxygen in the switching bottle, reduce the loss that causes the anaerobe bacterial at the switching in-process.

Preferably, the oxygen replacement mechanism comprises an air inlet pipe, an air outlet pipe, a first control valve and a second control valve, the air inlet pipe and the air outlet pipe are communicated with the bottle body, the first control valve is arranged on the air inlet pipe, the second control valve is arranged on the air outlet pipe, and the air inlet pipe is used for being communicated with an external nitrogen supply device.

Through adopting above-mentioned technical scheme, intercommunication intake pipe and outlet duct on the bottle, after putting into the bottle with anaerobism bacterial and sealing the bottleneck, feed through intake pipe and outside confession nitrogen equipment, then open first control valve and second control valve, utilize outside confession nitrogen equipment to pass through the air supply pipe and carry nitrogen gas in to the bottle, utilize the nitrogen gas that lets in to make the air that contains oxygen in the bottle discharge from the blast pipe, after the whole discharges of oxygen, close first control valve and second control valve, can accomplish the replacement to oxygen in the bottle.

Preferably, a filtering membrane for filtering nitrogen is arranged in the air inlet pipe.

Through adopting above-mentioned technical scheme, set up filtration membrane in the intake pipe, utilize filtration membrane to filter the inside nitrogen gas that lets in the bottle, improve the purity that lets in nitrogen gas, reduce the possibility that miscellaneous fungus in the nitrogen gas got into the bottle.

Preferably, the bottle body is provided with a dissolved oxygen meter.

Through adopting above-mentioned technical scheme, set up the dissolved oxygen table on the bottle, utilize the dissolved oxygen table to detect the oxygen content in the bottle, can in time stop supplying nitrogen according to the oxygen content in the bottle that detects, be convenient for control the content of letting in nitrogen gas, reduce the waste to nitrogen gas.

Preferably, a pressure gauge is arranged on the bottle body.

Through adopting above-mentioned technical scheme, set up the manometer on the bottle, utilize the manometer to detect the pressure in the bottle to the staff controls the pressure in the bottle, reduces the too big and anaerobic bacteria bacterial that causes in the bottle of bottle internal pressure and causes the possibility of loss.

Preferably, the bottle cap is fixedly connected with a vent pipe, the vent pipe is communicated with the inside of the bottle cap, a third control valve is arranged on the vent pipe, and the vent pipe is communicated with external steam supply equipment.

Through adopting above-mentioned technical scheme, intercommunication air supply pipe on the bottle lid, before switching anaerobism bacterial, close first control valve, open second control valve and third control valve, utilize steam supply equipment to supply high-temperature steam in to the bottle, utilize the high-temperature steam that lets in to disinfect to the bottle inside, reduce the content of the inside miscellaneous fungus of bottle.

Preferably, the coaxial fixedly connected with go-between in the outside of breather pipe, the lateral wall slope of go-between sets up, the diameter that bottle lid one end was kept away from to the go-between is less than the go-between is close to the diameter of bottle lid one end.

Through adopting above-mentioned technical scheme, at the outside fixed connection go-between of breather pipe, when needs let in high-temperature steam to the bottle in, establish the steam pipe cover on the steam supply equipment in the outside of go-between, utilize the slope setting of go-between, make and establish the steam pipe cover and establish required power on the breather pipe and be less than the power of pulling out the steam pipe from the breather pipe, be convenient for with steam pipe and breather pipe intercommunication, and difficult the breaking away from behind the intercommunication, improve the stability after the intercommunication.

Preferably, a heat insulation pad is arranged on the outer side of the bottle body.

Through adopting above-mentioned technical scheme, set up the heat insulating mattress in the outside of bottle, utilize the heat insulating mattress to protect the staff, reduce the staff because of the mistake touches the possibility of scalding, security when improving the staff operation.

Preferably, a cold water pipe is arranged on the side wall of the bottle body.

Through adopting above-mentioned technical scheme, set up the cold water pipe on the lateral wall of bottle, after carrying out high temperature sterilization to the bottle, pour into cold water into to the cold water pipe, utilize to pour into cold water into and cool down the bottle, improve the cooling rate of bottle.

In summary, the present application at least includes the following beneficial technical effects:

1. the bottle body is provided with the oxygen replacement mechanism, after the anaerobic bacteria strains are placed in the bottle body, the bottle cap is used for sealing the bottle opening, then nitrogen is supplied into the bottle body through the oxygen replacement mechanism, and the oxygen in the bottle body is replaced by the supplied nitrogen, so that the oxygen content in the transfer bottle is reduced, and the loss of the anaerobic bacteria strains in the transfer process is reduced;

2. the nitrogen is filtered by the filter screen in the air inlet pipe, so that the purity of the introduced nitrogen is improved, and the possibility that the nitrogen contains mixed bacteria and enters the bottle body is reduced;

3. through intercommunication breather pipe on the bottle lid, before putting into the bottle with anaerobism bacterial species, with the steam pipe intercommunication of breather pipe and outside steam supply equipment, make steam supply equipment supply high temperature steam to the interior confession of bottle, utilize high temperature steam to disinfect to the bottle inside, reduce the content of the inside miscellaneous fungus of bottle.

Drawings

FIG. 1 is a schematic view of the overall structure of embodiment 1 of the present application;

FIG. 2 is a schematic view mainly showing the structure of a filtration membrane in example 1 of the present application;

fig. 3 is a schematic view of the overall structure of embodiment 2 of the present application.

Reference numerals are as follows: 100. a bottle body; 200. a bottle cap; 300. an oxygen replacement mechanism; 310. an air inlet pipe; 320. an air outlet pipe; 330. a first control valve; 340. a second control valve; 350. a filtration membrane; 400. a dissolved oxygen meter; 500. a breather pipe; 510. a third control valve; 520. a connecting ring; 600. a pressure gauge; 700. a heat insulating pad; 800. a cold water pipe.

Detailed Description

The present application is described in further detail below with reference to figures 1-2.

The embodiment of the application discloses an anaerobe inoculation transfer bottle.

Example 1

Referring to fig. 1, an anaerobic bacteria inoculation adapter bottle includes a bottle body 100 and a bottle cap 200, a bottle opening is opened at an upper end of the bottle body 100, an external thread is opened at an outer side of the bottle body 100, the external thread is located at the bottle opening of the bottle body 100, an internal thread is opened at an inner side of the bottle cap 200, the external thread opened at the bottle body 100 is matched with the internal thread opened at the bottle cap 200, and the bottle cap 200 can be screwed to the bottle opening of the bottle body 100. The bottle 100 is installed with an oxygen replacement mechanism 300, and the oxygen replacement mechanism 300 is used to supply nitrogen gas into the bottle 100 to replace oxygen inside the bottle 100. In the process of transferring the anaerobic bacteria, after the anaerobic bacteria is put into the bottle body 100 from the bottle opening, the bottle cap 200 is screwed, nitrogen is supplied into the bottle body 100 through the oxygen replacement mechanism 300, and the oxygen in the bottle body 100 is replaced by the nitrogen, so that the content of the oxygen in the bottle body 100 is reduced, and the loss of the anaerobic bacteria in the transferring process is reduced. When the anaerobe strain in the transfer bottle needs to be transferred to the fermentation tank, the bottle opening on the bottle body 100 is communicated with the feeding hole on the fermentation tank, the pressure in the fermentation tank is changed, the negative pressure in the fermentation tank is enabled, and the anaerobe strain in the bottle body 100 can be pressed into the fermentation tank by utilizing the pressure difference.

Referring to fig. 1, the oxygen replacing mechanism 300 includes an inlet tube 310 and an outlet tube 320, both the inlet tube 310 and the outlet tube 320 are fixedly connected to the bottle body 100, the inlet tube 310 and the outlet tube 320 are respectively located at positions near both sides of the bottle body 100, and both the inlet tube 310 and the outlet tube 320 are communicated with the inside of the bottle body 100. The first control valve 330 is installed on the gas inlet pipe 310, the first control valve 330 is used for controlling the opening or closing of the gas inlet pipe 310, and one end of the gas inlet pipe 310, which is far away from the bottle 100, is used for communicating with an external nitrogen supply device. The outlet pipe 320 is provided with a second control valve 340, and the second control valve 340 is used for controlling the opening or closing of the outlet pipe 320. The bottle body 100 is provided with a dissolved oxygen meter 400, and the dissolved oxygen meter 400 is used for detecting the oxygen content in the bottle body 100. After putting into bottle 100 with anaerobe bacterial, open first control valve 330 and second control valve 340, utilize outside confession nitrogen equipment to supply nitrogen gas in to bottle 100 through intake pipe 310, make the air that contains oxygen in the bottle 100 pass through the exhaust of outlet duct 320, realize the replacement of oxygen in the bottle 100, oxygen in to bottle 100 is detected through dissolved oxygen table 400, be convenient for in time close first control valve 330 and second control valve 340, the reduction is to the consumption of nitrogen gas.

Referring to fig. 1 and 2, a filtering membrane 350 is fixedly connected to the inside of the inlet pipe 310 in order to ensure the quality of the introduced nitrogen gas, and the filtering membrane 350 is used for filtering the foreign bacteria contained in the nitrogen gas. The filtering membrane 350 is used for removing the mixed bacteria in the nitrogen, so that the possibility of the mixed bacteria in the nitrogen entering the bottle body 100 is reduced.

Referring to fig. 1, a pressure gauge 600 is installed on the bottle 100, the pressure gauge 600 is used for detecting the pressure inside the bottle 100, and a worker can control the pressure inside the bottle 100 according to the data detected by the pressure gauge 600, so that the possibility of anaerobic bacteria strain loss inside the bottle 100 caused by excessive pressure inside the bottle 100 can be reduced.

The implementation principle of an anaerobe inoculation transfer bottle of the embodiment of the application is as follows: through installing intake pipe 310 and outlet duct 320 on bottle 100, and install first control valve 330 on intake pipe 310, install second control valve 340 on outlet duct 320, after putting into bottle 100 with anaerobe bacterial, communicate intake pipe 310 with outside confession nitrogen equipment, then open first control valve 330 and second control valve 340, utilize outside confession nitrogen equipment to supply nitrogen gas in the bottle 100, thereby make the oxygen in the bottle 100 discharge from outlet duct 320, reduce the content of oxygen in the switching bottle, thereby reduce the loss that causes the anaerobe bacterial in the switching process.

Example 2

Referring to fig. 3, the embodiment of the present application differs from embodiment 1 in that: the top fixedly connected with breather pipe 500 of bottle lid 200, the vertical setting of breather pipe 500, the inside intercommunication of breather pipe 500 and bottle lid 200, the breather pipe 500 one end of keeping away from bottle lid 200 be used for with the steam pipe intercommunication on the steam supply equipment of outside, steam supply equipment is used for supplying high-temperature steam to the transfer bottle. A third control valve 510 is installed on the ventilation pipe 500, and the third control valve 510 is used for controlling the opening or closing of the ventilation pipe 500. Before the anaerobic bacteria are put into the bottle body 100, the third control valve 510 and the second control valve 340 are opened, so that the external steam supply device supplies high-temperature steam into the bottle body 100 through the vent pipe 500, and the high-temperature steam is used for sterilizing the interior of the bottle body 100, thereby reducing the content of the bacteria in the interior of the bottle body 100.

Referring to fig. 3, a plurality of coupling rings 520 are coaxially and fixedly coupled to an outer side of the breather pipe 500, and the plurality of coupling rings 520 are spaced apart from each other in an axial direction of the breather pipe 500. Each of the connection rings 520 has a sidewall inclined, and the diameter of the upper end of the connection ring 520 is smaller than that of the lower end of the connection ring 520. When high-temperature steam needs to be introduced into the bottle body 100, the steam pipes on the steam supply device are sleeved on the connecting rings 520, so that the communication between the steam pipes on the steam supply device and the vent pipe 500 is realized, when the steam supply device is installed, because each connecting ring 520 is obliquely arranged, the force for sleeving the steam pipes on the vent pipe 500 is smaller than the force for pulling the steam pipes out of the vent pipe 500, the steam pipes are conveniently communicated with the vent pipe 500, and the steam pipes are not easy to separate after being communicated.

Referring to fig. 3, a cold water pipe 800 is fixedly connected to the bottle body 100, the cold water pipe 800 is spirally wound on the outer side of the bottle body 100, a heat insulation pad 700 is sleeved on the outer side of the cold water pipe 800, the heat insulation pad 700 is fixedly connected with the cold water pipe 800, the bottle body 100 is isolated from the outside by the heat insulation pad 700, the possibility of scalding due to mistakenly touching the bottle body 100 in the use process is reduced, and the safety of the adapter bottle in use is improved. After the high-temperature steam is introduced into the bottle body 100 to sterilize the interior of the bottle body 100, cold water is introduced into the cold water pipe 800, the cooling speed of the bottle body 100 is increased by using the cold water, and the cooling rate of the bottle body 100 is increased.

The implementation principle of an anaerobic bacteria inoculation transfer bottle in the embodiment of the application is as follows: through intercommunication breather pipe 500 on bottle lid 200, utilize steam supply equipment to supply high temperature steam to the bottle 100 in through breather pipe 500, utilize high temperature steam to carry out high temperature sterilization to the bottle 100 inside, reduce the content of the inside miscellaneous fungus of bottle 100 to reduce the possibility that causes the pollution to the bacterial in the anaerobe bacterial switching process.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims (9)

1. An anaerobe inoculation transfer bottle which is characterized in that: the oxygen bottle comprises a bottle body (100), a bottle cap (200) and an oxygen replacement mechanism (300), wherein a bottle opening is formed in the bottle body (100), the bottle cap (200) is used for sealing or opening the bottle opening, the oxygen replacement mechanism (300) is connected with the bottle body (100), and the oxygen replacement mechanism (300) is used for supplying nitrogen to the bottle body (100) so as to replace oxygen in the bottle body (100).

2. The anaerobic bacteria inoculation transfer bottle according to claim 1, characterized in that: oxygen replacement mechanism (300) includes intake pipe (310), outlet duct (320), first control valve (330) and second control valve (340), intake pipe (310) with outlet duct (320) all with bottle (100) intercommunication, first control valve (330) set up on intake pipe (310), second control valve (340) set up on outlet duct (320), intake pipe (310) are used for with outside confession nitrogen equipment intercommunication.

3. The anaerobic bacteria inoculation transfer bottle according to claim 2, characterized in that: a filtering membrane (350) for filtering nitrogen is arranged in the air inlet pipe (310).

4. The anaerobe inoculation transfer bottle of claim 2, wherein: the bottle body (100) is provided with a dissolved oxygen meter (400).

5. The anaerobe inoculation transfer bottle of claim 2, wherein: the bottle body (100) is provided with a pressure gauge (600).

6. The anaerobe inoculation transfer bottle of claim 1, wherein: fixedly connected with breather pipe (500) on bottle lid (200), breather pipe (500) with bottle lid (200) inside intercommunication, be provided with third control valve (510) on breather pipe (500), breather pipe (500) are used for with outside steam supply equipment intercommunication.

7. The anaerobe inoculation transfer bottle of claim 6, wherein: the coaxial fixedly connected with go-between (520) in the outside of breather pipe (500), the lateral wall slope setting of go-between (520), the diameter that bottle lid (200) one end was kept away from in go-between (520) is less than go-between (520) are close to the diameter of bottle lid (200) one end.

8. The anaerobe inoculation transfer bottle of claim 6, wherein: the outer side of the bottle body (100) is provided with a heat insulation pad (700).

9. The anaerobic bacteria inoculation transfer bottle of claim 6, which is characterized in that: the side wall of the bottle body (100) is provided with a cold water pipe (800).

Images