CN108188094B - Automatic film washing machine for western blot test - Google Patents
Automatic film washing machine for western blot test Download PDFInfo
- Publication number
- CN108188094B CN108188094B CN201810109032.3A CN201810109032A CN108188094B CN 108188094 B CN108188094 B CN 108188094B CN 201810109032 A CN201810109032 A CN 201810109032A CN 108188094 B CN108188094 B CN 108188094B
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- China
- Prior art keywords
- pipeline
- liquid
- waste liquid
- membrane
- bottom plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000005406 washing Methods 0.000 title claims abstract description 52
- 238000001262 western blot Methods 0.000 title claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 80
- 239000012528 membrane Substances 0.000 claims abstract description 54
- 239000002699 waste material Substances 0.000 claims abstract description 34
- 238000000746 purification Methods 0.000 claims abstract description 22
- 239000011550 stock solution Substances 0.000 claims description 12
- 238000005057 refrigeration Methods 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 5
- 239000002033 PVDF binder Substances 0.000 abstract description 7
- 229920002981 polyvinylidene fluoride Polymers 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 6
- 238000003119 immunoblot Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
- B08B3/14—Removing waste, e.g. labels, from cleaning liquid; Regenerating cleaning liquids
Landscapes
- Cleaning By Liquid Or Steam (AREA)
Abstract
The invention relates to the field of life science, in particular to an automatic membrane washing machine for a western blot test, which comprises a bottom plate, a supporting plate, a centrifugal purification device, a raw liquid placing barrel and a waste liquid collecting barrel, wherein a balance supporting device and a shaking motor are arranged on the bottom plate, the supporting plate is arranged on the bottom plate, a membrane washing box is arranged on the supporting plate, a first pipeline is arranged between the raw liquid placing barrel and the membrane washing box, a negative pressure pump is arranged at the top of the waste liquid collecting barrel, a second pipeline is arranged between the negative pressure pump and the membrane washing box, the centrifugal purification device is positioned beside the supporting plate, a third pipeline is arranged between the raw liquid placing barrel and the centrifugal purification device, a fourth pipeline is arranged between the waste liquid collecting barrel and the centrifugal purification device, a first liquid extracting pump is arranged on the first pipeline, an electromagnetic valve is arranged on the second pipeline, a second liquid extracting pump is arranged on the third pipeline, and a third liquid extracting pump is arranged on the fourth pipeline. The membrane washing liquid can be recycled, the cost is saved, and the membrane washing liquid has better effect of washing PVDF membrane by shaking the membrane washing box through the shaking motor.
Description
Technical Field
The invention relates to the field of life science, in particular to an automatic film washing machine for a western blot test.
Background
Immunoblotting assays, which detect the amount of a certain protein in a sample, are common test items. Immunoblotting assays for detecting the amount of a protein in a sample are common techniques for detecting the amount of a specific protein in biological samples, and are common means for biological testing companies. Antibodies used in immunoblotting assays for detecting the amount of a protein in a sample are highly productive and extremely expensive, and scientific researchers spend a great deal of money on purchasing antibodies each year. In a traditional immunoblotting test for detecting a protein content in a sample, a PVDF membrane is generally incubated in a common incubation box, and only about 4 milliliters of antibody diluent is required to cover the sheared PVDF membrane. The potency of the used antibody diluent after recovery can be greatly reduced, and the waste can cause great economic loss, thereby aggravating the test cost. In order to save the cost, researchers use a film washing machine to wash and recycle PVDF films, but the traditional film washing machine can only shake left and right, so that the film washing effect is poor.
Disclosure of Invention
The invention aims to provide an automatic film washing machine for a western blot test, aiming at overcoming the defects of the prior art.
In order to solve the problems, the invention provides the following technical scheme:
the utility model provides a western blot is experimental with automatic membrane washing machine, includes bottom plate, backup pad, centrifugal purification device, stoste place bucket and waste liquid collecting vessel, be equipped with balanced strutting arrangement and shake motor on the bottom plate, the backup pad is installed on balanced strutting arrangement and shake motor, be equipped with the washing membrane box in the backup pad, stoste is placed bucket and waste liquid collecting vessel and is located the both sides of bottom plate respectively, be equipped with a plurality of between stoste and the washing membrane box and be used for the intercommunication stoste to place bucket and washing membrane box first pipeline, the top of waste liquid collecting vessel is equipped with the negative pressure pump, be equipped with a plurality of between negative pressure pump and the washing membrane box and be used for the second pipeline of intercommunication negative pressure pump and washing membrane box, centrifugal purification device is located the side of backup pad, be equipped with the third pipeline that is used for the intercommunication stoste to place bucket and centrifugal purification device between stoste collecting vessel and the centrifugal purification device, every all be equipped with first drawing liquid pump on the first pipeline, every second pipeline is equipped with the fourth drawing liquid pump on the third pipeline, all be equipped with the fourth drawing liquid pump on the third pipeline.
Further, the balance support device comprises four hydraulic balance rods which are in rectangular distribution, a first ball bearing is arranged on the bottom plate corresponding to each hydraulic balance rod, a second ball bearing is arranged on the bottom of the support plate corresponding to each hydraulic balance rod, the upper ends of the hydraulic balance rods are fixedly mounted on the corresponding second ball bearings, and the lower ends of the hydraulic balance rods are fixedly mounted on the corresponding first ball bearings.
Further, the bottom of backup pad is equipped with the third ball bearing, shake motor's output and be vertical setting up, third ball bearing fixed mounting is on shake motor's output.
Further, the bottom plate is also provided with a controller, the controller is provided with a display screen connected with the controller, the inside of the washing box is provided with a refrigeration temperature control component, and the refrigeration temperature control component, the shaking motor, the electromagnetic valve, the first liquid pump, the second liquid pump and the third liquid pump are electrically connected with the controller.
Further, the top of the stock solution placing barrel is also provided with an infrared liquid level sensor and a barrel cover, and the infrared liquid level sensor is electrically connected with the controller.
Further, the top of waste liquid collecting vessel is equipped with the solid fixed ring with the inside intercommunication of waste liquid collecting vessel, negative pressure pump fixed mounting is on solid fixed ring.
Further, the stock solution placing barrel is provided with a scale strip.
The beneficial effects are that: when the automatic membrane washing machine for the western blot test is used, the first liquid pump on the first pipeline pumps membrane washing liquid in the stock solution placing barrel into the membrane washing box, then the shaking motor drives the supporting plate to shake, the membrane washing box also shakes along with the membrane washing box, the PVDF membrane in the membrane washing box is gradually washed, then the electromagnetic valve on the second pipeline is opened, the negative pressure pump works to pump waste liquid in the membrane washing box into the waste liquid collecting barrel, then the third liquid pump on the fourth pipeline is opened to pump waste liquid in the waste liquid collecting barrel into the centrifugal purification device, the centrifugal purification device purifies the waste liquid into usable membrane washing liquid, and then the second liquid pump pumps membrane washing liquid in the centrifugal purification device into the stock solution placing barrel.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is an enlarged view of FIG. 1 at A;
FIG. 3 is a side view of the present invention;
reference numerals illustrate: the device comprises a bottom plate 1, a first ball bearing 1a, a controller 1b, a display screen 1c, a supporting plate 2, a second ball bearing 2a, a third ball bearing 2b, a centrifugal purification device 3, a stock solution placing barrel 4, a barrel cover 4a, a waste liquid collecting barrel 5, a negative pressure pump 5a, a balance supporting device 6, a hydraulic balance rod 6a, a shaking motor 7, a washing box 8, a first pipeline 9, a first liquid pump 9a, a second pipeline 10, an electromagnetic valve 10a, a third pipeline 11, a second liquid pump 11a, a fourth pipeline 12 and a third liquid pump 12a.
Description of the embodiments
Specific embodiments of the invention are described in further detail below with reference to the drawings and examples of the specification:
referring to the automatic membrane washer for the western blot test shown in fig. 1 to 3, the membrane washer comprises a base plate 1, a support plate 2, a centrifugal purification device 3, a raw liquid placing barrel 4 and a waste liquid collecting barrel 5, wherein a balance support device 6 and a shaking motor 7 are arranged on the base plate 1, the support plate 2 is arranged on the balance support device 6 and the shaking motor 7, a membrane washing box 8 is arranged on the support plate 2, the raw liquid placing barrel 4 and the waste liquid collecting barrel 5 are respectively positioned on two sides of the base plate 1, a plurality of first pipelines 9 for communicating the raw liquid placing barrel 4 with the membrane washing box 8 are arranged between the raw liquid placing barrel 4 and the membrane washing box 8, a negative pressure pump 5a is arranged at the top of the waste liquid collecting barrel 5, a plurality of second pipelines 10 for communicating the negative pressure pump 5a with the membrane washing box 8 are arranged between the negative pressure pump 5a and the membrane washing box 8, the centrifugal purification device 3 is positioned on the side of the support plate 2, a third pipeline 11 for communicating the raw liquid placing barrel 4 with the centrifugal purification device 3 is arranged between the raw liquid placing barrel 4 and the centrifugal purification device 3, a fourth pump 11 is arranged between the first pipelines 11 and the centrifugal purification device 3, and the fourth pipelines 12 are respectively arranged on the third pipelines 11, and the fourth pipelines 11 are respectively arranged between the third pipelines are respectively, and are provided with a pump 12.
Working principle: the utility model discloses when using, first drawing liquid pump 9a on the first pipeline 9 places the membrane cleaning solution in the bucket 4 in the membrane cleaning box 8 with the stoste, shake motor 7 drive backup pad 2 shake afterwards, membrane cleaning box 8 also shakes thereupon, PVDF membrane in the membrane cleaning box 8 is washed gradually, then solenoid valve 10a on the second pipeline 10 opens, negative pressure pump 5a work is with the waste liquid extraction in the membrane cleaning box 8 in the waste liquid collecting vessel 5, then third drawing liquid pump 12a on the fourth pipeline 12 opens and draws centrifugal purifier 3 with the waste liquid in the waste liquid collecting vessel 5, centrifugal purifier 3 purifies the waste liquid into the membrane cleaning solution that can use, then second drawing liquid pump 11a is with the membrane cleaning solution in the centrifugal purifier 3 draw to the stoste place in the bucket 4, thereby the membrane cleaning solution can recycle, the cost is saved, and the effect that membrane cleaning solution washs PVDF membrane through shake of membrane cleaning box 8 of shake motor 7 is better.
Referring to fig. 1 and 2, the balance supporting device 6 includes four rectangular hydraulic balance bars 6a, a first ball bearing 1a is disposed on the bottom plate 1 corresponding to each hydraulic balance bar 6a, a second ball bearing 2a is disposed on the bottom of the supporting plate 2 corresponding to each hydraulic balance bar 6a, an upper end of each hydraulic balance bar 6a is fixedly mounted on the corresponding second ball bearing 2a, and a lower end of each hydraulic balance bar 6a is fixedly mounted on the corresponding first ball bearing 1 a; when the shaking motor 7 drives the supporting plate 2 to shake, the four hydraulic balance bars 6a keep the supporting plate 2 balanced, so that the washing film box 8 is also kept balanced.
Referring to fig. 3, a third ball bearing 2b is disposed at the bottom of the supporting plate 2, the output end of the shaking motor 7 is disposed vertically upwards, and the third ball bearing 2b is fixedly mounted on the output end of the shaking motor 7; the driving motor drives the third ball bearing 2b to shake, and the support plate 2 shakes with the shake of the third ball bearing 2 b.
Referring to fig. 1, the bottom plate 1 is further provided with a controller 1b, the controller 1b is provided with a display screen 1c connected with the controller 1b, a refrigeration temperature control component is arranged in the washing chamber 8, and the refrigeration temperature control component, the shaking motor 7, the electromagnetic valve 10a, the first liquid pump 9a, the second liquid pump 11a and the third liquid pump 12a are all electrically connected with the controller 1 b; the controller 1b can control the opening and closing of the related mechanisms, the display screen 1c can set the film washing time and the film washing liquid content each time, and can also control the film washing temperature and the film washing times.
Referring to fig. 1, the top of the stock solution placing barrel 4 is also provided with an infrared liquid level sensor and a barrel cover 4a, and the infrared liquid level sensor is electrically connected with a controller 1 b; when the infrared liquid level sensor detects that the stock solution in the stock solution placing barrel 4 is insufficient, the controller 1b controls the second liquid pump 11a to pump the membrane washing liquid from the centrifugal purification device 3 to supplement the membrane washing liquid into the stock solution placing barrel 4.
Referring to fig. 1, a fixing ring is arranged at the top of the waste liquid collecting barrel 5 and is communicated with the interior of the waste liquid collecting barrel 5, and the negative pressure pump 5a is fixedly arranged on the fixing ring; when the solenoid valve 10a is opened, the negative pressure pump 5a can pump the waste liquid in the washing chamber 8 into the waste liquid collection tank.
The stock solution placing barrel 4 is provided with scale bars; the design of scale strip has made things convenient for research and development personnel to observe the surplus of membrane washing liquid in the stoste placing barrel 4.
The foregoing description is only a preferred embodiment of the present invention, and is not intended to limit the technical scope of the present invention, so any minor modifications, equivalent changes and modifications made to the above embodiments according to the technical principles of the present invention are still within the scope of the technical solutions of the present invention.
Claims (1)
1. The utility model provides a western blot is automatic membrane machine of washing for test which characterized in that: including bottom plate (1), backup pad (2), centrifugal purification device (3), stoste place bucket (4) and waste liquid collecting vessel (5), be equipped with balanced strutting arrangement (6) and shake motor (7) on bottom plate (1), backup pad (2) are installed on balanced strutting arrangement (6) and shake motor (7), be equipped with on backup pad (2) and wash membrane box (8), stoste place bucket (4) and waste liquid collecting vessel (5) are located the both sides of bottom plate (1) respectively, be equipped with first pipeline (9) that a plurality of was used for communicating stoste place bucket (4) and washing membrane box (8) between stoste place bucket (4) and washing membrane box (8), the top of waste liquid collecting vessel (5) is equipped with negative pressure pump (5 a), be equipped with second pipeline (10) that a plurality of was used for communicating negative pressure pump (5 a) and membrane box (8) between negative pressure pump (5 a) and washing membrane box (8), stoste place bucket (4) and centrifugal purification device (3) are located the both sides of bottom plate (1), stoste place bucket (4) and centrifugal purification device (3) are equipped with between the stoste and centrifugal purification device (11), a fourth pipeline (12) for communicating the waste liquid collecting barrel (5) with the centrifugal purifying device (3) is arranged between the waste liquid collecting barrel (5) and the centrifugal purifying device (3), a first liquid-extracting pump (9 a) is arranged on each first pipeline (9), an electromagnetic valve (10 a) is arranged on each second pipeline (10), a second liquid-extracting pump (11 a) is arranged on each third pipeline (11), and a third liquid-extracting pump (12 a) is arranged on each fourth pipeline (12); the balance supporting device (6) comprises four rectangular hydraulic balance rods (6 a), a first ball bearing (1 a) is arranged on the bottom plate (1) corresponding to each hydraulic balance rod (6 a), a second ball bearing (2 a) is arranged at the bottom of the supporting plate (2) corresponding to each hydraulic balance rod (6 a), the upper end of each hydraulic balance rod (6 a) is fixedly arranged on the corresponding second ball bearing (2 a), and the lower end of each hydraulic balance rod (6 a) is fixedly arranged on the corresponding first ball bearing (1 a); the bottom of the supporting plate (2) is provided with a third ball bearing (2 b), the output end of the shaking motor (7) is vertically upwards, and the third ball bearing (2 b) is fixedly arranged on the output end of the shaking motor (7); the bottom plate (1) is also provided with a controller (1 b), the controller (1 b) is provided with a display screen (1 c) connected with the controller (1 b), the inside of the washing box (8) is provided with a refrigeration temperature control component, and the refrigeration temperature control component, the shaking motor (7), the electromagnetic valve (10 a), the first liquid-extracting pump (9 a), the second liquid-extracting pump (11 a) and the third liquid-extracting pump (12 a) are electrically connected with the controller (1 b); the top of the waste liquid collecting barrel (5) is provided with a fixed ring communicated with the inside of the waste liquid collecting barrel (5), and the negative pressure pump (5 a) is fixedly arranged on the fixed ring; the stock solution placing barrel (4) is provided with scale strips; the top of the stock solution placing barrel (4) is also provided with an infrared liquid level sensor and a barrel cover (4 a), and the infrared liquid level sensor is electrically connected with the controller (1 b).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810109032.3A CN108188094B (en) | 2018-02-05 | 2018-02-05 | Automatic film washing machine for western blot test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810109032.3A CN108188094B (en) | 2018-02-05 | 2018-02-05 | Automatic film washing machine for western blot test |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108188094A CN108188094A (en) | 2018-06-22 |
| CN108188094B true CN108188094B (en) | 2024-02-23 |
Family
ID=62592386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810109032.3A Active CN108188094B (en) | 2018-02-05 | 2018-02-05 | Automatic film washing machine for western blot test |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108188094B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110850098B (en) * | 2019-11-21 | 2023-11-14 | 中国人民解放军西部战区总医院 | Automatic film washing device for Western blot experiment |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000107574A (en) * | 1998-10-05 | 2000-04-18 | Kubota Corp | Method and apparatus for washing membrane cartridge with chemical solution |
| CN203853330U (en) * | 2014-03-29 | 2014-10-01 | 云南滇溪水业发展股份有限公司 | Ultrasonic drinking water barrel cleaning device |
| CN204448674U (en) * | 2015-02-11 | 2015-07-08 | 崔楠 | A kind of physical education basketball cleaning device |
| CN204583757U (en) * | 2015-04-29 | 2015-08-26 | 珠海健帆生物科技股份有限公司 | Blood purification consumptive material clean cycle system |
| CN205413785U (en) * | 2016-02-28 | 2016-08-03 | 罗远烽 | Clean processing apparatus of bio -pharmaceuticals experimental device |
| CN205988933U (en) * | 2016-08-26 | 2017-03-01 | 孔德光 | Film device is washed by a kind of automatization of Western blot experiment |
| CN106733886A (en) * | 2016-12-20 | 2017-05-31 | 上海长海医院 | A kind of full automatic high efficiency western blot wash membranous system |
| CN107175235A (en) * | 2017-07-26 | 2017-09-19 | 中铁第四勘察设计院集团有限公司 | Injection combines the hydraulic oil container circulation cleaning device and method of ultrasonic wave |
| CN206706418U (en) * | 2017-03-29 | 2017-12-05 | 阙优标 | A kind of dehydrating vessel in washing machine bascule of Simple And Practical |
| CN209205914U (en) * | 2018-02-05 | 2019-08-06 | 朱宏明 | A kind of automatic Film washer of WB |
-
2018
- 2018-02-05 CN CN201810109032.3A patent/CN108188094B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000107574A (en) * | 1998-10-05 | 2000-04-18 | Kubota Corp | Method and apparatus for washing membrane cartridge with chemical solution |
| CN203853330U (en) * | 2014-03-29 | 2014-10-01 | 云南滇溪水业发展股份有限公司 | Ultrasonic drinking water barrel cleaning device |
| CN204448674U (en) * | 2015-02-11 | 2015-07-08 | 崔楠 | A kind of physical education basketball cleaning device |
| CN204583757U (en) * | 2015-04-29 | 2015-08-26 | 珠海健帆生物科技股份有限公司 | Blood purification consumptive material clean cycle system |
| CN205413785U (en) * | 2016-02-28 | 2016-08-03 | 罗远烽 | Clean processing apparatus of bio -pharmaceuticals experimental device |
| CN205988933U (en) * | 2016-08-26 | 2017-03-01 | 孔德光 | Film device is washed by a kind of automatization of Western blot experiment |
| CN106733886A (en) * | 2016-12-20 | 2017-05-31 | 上海长海医院 | A kind of full automatic high efficiency western blot wash membranous system |
| CN206706418U (en) * | 2017-03-29 | 2017-12-05 | 阙优标 | A kind of dehydrating vessel in washing machine bascule of Simple And Practical |
| CN107175235A (en) * | 2017-07-26 | 2017-09-19 | 中铁第四勘察设计院集团有限公司 | Injection combines the hydraulic oil container circulation cleaning device and method of ultrasonic wave |
| CN209205914U (en) * | 2018-02-05 | 2019-08-06 | 朱宏明 | A kind of automatic Film washer of WB |
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| Publication number | Publication date |
|---|---|
| CN108188094A (en) | 2018-06-22 |
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