CN205988933U - Film device is washed by a kind of automatization of Western blot experiment - Google Patents
Film device is washed by a kind of automatization of Western blot experiment Download PDFInfo
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- CN205988933U CN205988933U CN201620944338.7U CN201620944338U CN205988933U CN 205988933 U CN205988933 U CN 205988933U CN 201620944338 U CN201620944338 U CN 201620944338U CN 205988933 U CN205988933 U CN 205988933U
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- box body
- holding groove
- liquid holding
- tbst
- automatization
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Abstract
The utility model discloses film device is washed by a kind of automatization of Western blot experiment，Including the first box body，The upper end of described first box body connects the second box body，And first box body inner chamber side in be provided with TBST cleanout fluid storage tank，It is simple that film device structure is washed by this automatization，Whole liquid holding groove is controlled to be vacillated now to the left, now to the right using cam structure，So that internal band is cleaned fully，This device adopts embedded controller to realize the control of the electromagnetic valve to whole device，Method is to set scavenging period and wash number using control panel，Embedded controller carries timer module using it after obtaining parameter and carries out time cumulation，The liquid holding groove liquid level information then detecting with reference to liquid level sensor in liquid holding groove level and wash number information control the closure of electromagnetic valve，Thus automatically controlling the multiple turnover of TBST cleanout fluid，Greatly save the time，Improve work efficiency.
Description
Technical field
This utility model is related to technical field of medical equipment, specially a kind of automatization of Western blot experiment
Wash film device.
Background technology
Western blotting（Western blot test）I.e. Western Blot.It is molecular biology, biochemistry and exempts from
Conventional a kind of experimental technique in epidemic disease hereditism.Its ultimate principle is cell gel electrophoresiss being processed by specific antibody
Or biological tissue samples are coloured.Thin analyzed with color depth acquisition specified protein by analyzing the position colouring
The information of expression in born of the same parents or tissue.
Western blotting is by biological study institute uncommon in Switzerland's meter Xie Er Fred（Friedrich Miescher
Institute）Harry Towbin 1979 propose.In Neil Bai Naite（Neal Burnette）In 1981
Written《Analytical biochemistry》（Analytical Biochemistry）In be referred to as Western Blot first.Albumen is exempted from
Epidemic disease trace（Western Blot）It is that the cell or tissue gross protein after electrophoretic separation is transferred to solid support from gel
On NC film or pvdf membrane, then use a kind of protein detection techniques of detection of specific antibody specific antigen, extensively should
Detect and the many aspects such as disease early diagnosiss in the expression study of protein level, antibody activity for gene.
Protein blot experiment whole process includes protein sample makings, electrophoresis, transferring film, closing, is incubated and one resists, wash film, incubate
Educate the steps such as two anti-, colour developings；Although to wash the membrane process time shorter in whole step, but this process is repeated, foolproof
Artificial operation.The method commonly used both at home and abroad at present is to put into band in the box filling TBST, box is put on shaking table and shakes
Shake, change once new TBST every 5-10 minute, altogether will wash 3 times；It is intended to wash film, whole experiment is done before and after incubation two is anti-
Get off, the number of times changing TBST is up to 6 times, and the interval time changed every time is very short, less than 10 minutes, repeatedly frequently changes
Wash, bring inconvenience to research worker.
Utility model content
The purpose of this utility model is to provide a kind of automatization of Western blot experiment to wash film device, to solve
The problem certainly proposing in above-mentioned background technology.
For achieving the above object, this utility model provides following technical scheme：
Film device is washed by a kind of automatization of Western blot experiment, including the first box body, described first box body upper
It is provided with TBST cleanout fluid storage tank in end connection the second box body, and the inner chamber side of the first box body, and TBST cleanout fluid storage tank
Connected by one end insertion of feed tube and liquid holding groove, feed tube is provided with the first electromagnetic valve and the first water pump, described liquid holding groove
Rotated by cylinder and the second box body and connect, and between liquid holding groove and the second box body, be provided with cam gear and can be contained with location control
The angle of inclination of liquid bath, cam gear is provided with motor, and the bottom of motor is fixedly connected the upper surface of the second box body, described
The other end of liquid holding groove passes through drain pipe and TBST devil liquor recovery tank insertion connects, and drain pipe is provided with the second electromagnetic valve and second
Water pump, and TBST devil liquor recovery tank be located at the first box body opposite side inner chamber in, described first electromagnetic valve, the second electromagnetic valve and electricity
Motivation all with embedded controller be electrically connected with, and embedded controller simultaneously with control panel, liquid level detecting sensor, alarm bell
And display lamp is electrically connected with, described embedded controller, alarm bell and display lamp are located at the inside of the second box body, and control panel is solid
Surely it is connected to the lateral wall of the second box body, liquid level detecting sensor is disposed vertically in liquid holding groove.
Preferably, the bottom of described first box body is fixedly connected four support columns, and the bottom of support column is provided with anti-slip veins.
Preferably, described cam gear includes a major axis and four cams, four cams all fixedly sleeved on major axis.
Preferably, described first box body and the second box body are rectangular structure, and cylinder is located in the bottom of liquid holding groove
Centre position.
Preferably, described control panel adopts button setting and liquid crystal display, and control panel is fixedly connected on the second box body
Lateral wall.
Compared with prior art, the beneficial effects of the utility model are：The automatization of this Western blot experiment washes
Film device structure is simple, controls whole liquid holding groove to be vacillated now to the left, now to the right using cam structure, so that internal band cleaning is filled
Point, by adding TBST cleanout fluid storage tank and TBST devil liquor recovery tank at the two ends of the first box body, in cleaning plus TBST cleaning
During liquid, motor control cam makes liquid holding groove be the level of state and adopts feed tube and the first solenoid valve vent mechanism, is reclaiming
Same holding liquid holding groove level during TBST waste liquid, using drain pipe and the second solenoid valve vent structure, using embedded controller
The electromagnetic valve of whole device is controlled, sets time interval and the wash number of intervalometer, using in liquid holding groove
Liquid level detecting sensor obtains liquid level information and carries out closed loop control, thus automatically controlling the turnover of TBST cleanout fluid, it is to avoid people
The numerous trouble changing liquid of power frequency, greatlys save the time, improves work efficiency.
Brief description
Fig. 1 is this utility model structural front view；
Fig. 2 is this utility model structure top view；
Fig. 3 is embedded controller workflow diagram of the present utility model.
In figure：1 first box body, 2 TBST cleanout fluid storage tanks, 3 feed tubes, 4 first electromagnetic valves, 5 liquid holding grooves, 6 go out liquid
Pipe, 7 second electromagnetic valves, 8 TBST devil liquor recovery tanks, 9 cylinders, 10 cam gears, 11 embedded controllers, 12 alarm bells, 13 fingers
Show lamp, 14 control panels, 15 second box bodys, 16 liquid level detecting sensors.
Specific embodiment
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out
Clearly and completely description is it is clear that described embodiment is only a part of embodiment of this utility model rather than whole
Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under the premise of making creative work
The every other embodiment being obtained, broadly falls into the scope of this utility model protection.
Refer to Fig. 1-2, this utility model provides a kind of technical scheme：
Film device is washed by a kind of automatization of Western blot experiment, including the first box body 1, described first box body 1
It is provided with TBST cleanout fluid storage tank 2 in upper end connection the second box body 15, and the inner chamber side of the first box body 1, and TBST cleanout fluid
Storage tank 2 passes through feed tube 3 and one end insertion of liquid holding groove 5 connects, and liquid holding groove 5 includes liquid level detecting sensor 16, feed tube 3
It is provided with the first electromagnetic valve 4 and the first water pump, the first electromagnetic valve 4 is fixedly connected with the side of the first water pump and the first box body 1, institute
State liquid holding groove 5 and pass through cylinder 9 and the second box body 15 rotation connection, and between liquid holding groove 5 and the second box body 15, be provided with cam dress
Put 10, cam gear 10 is provided with motor, the bottom of motor is fixedly connected the upper surface of the second box body 15, described liquid holding groove
5 other end passes through drain pipe 6 and TBST devil liquor recovery tank 8 insertion connects.
Drain pipe 6 is provided with the second electromagnetic valve 7 and the second water pump, the second electromagnetic valve 7 and the second water pump and the first box body 1
Another side is fixedly connected, and TBST devil liquor recovery tank 8 be located at the first box body 1 opposite side inner chamber in, described first electromagnetism
Valve 4, the second electromagnetic valve 7 and motor are all electrically connected with embedded controller 11, and embedded controller 11 simultaneously with control
Panel 14, alarm bell 12, display lamp 13 and liquid level detecting sensor 16 are electrically connected with, described embedded controller 11, alarm bell 12
It is located at the inside of the second box body 15 with display lamp 13, control panel 14 is fixedly connected on the lateral wall of the second box body 15, described the
The bottom of one box body 1 is fixedly connected four support columns, and the bottom of support column is provided with anti-slip veins, and described cam gear 10 includes one
Root major axis and four cams, four cams all fixedly sleeved on major axis, described first box body 1 and the second box body 15 are rectangular
Body structure, cylinder 9 is located at the lower center position of liquid holding groove 5.
Operation principle：Cam rotation special angle control realization liquid holding groove level, the first electromagnetic valve 4 and the under naturalness
Two electromagnetic valves 7 are all closures, when needing cleaning, set time and wash number first with control panel 14, then
Band to be cleaned is put in liquid holding groove 5, now, input cleanout fluid flows into instruction, and embedded controller 11 can control first
Electromagnetic valve 4 and the first starting mode of pump, TBST cleanout fluid will flow through feed tube 3 from TBST cleanout fluid storage tank 2, reach liquid holding groove
Until liquid level detecting sensor 16 detects preferable liquid level information in 5, then, embedded controller 11 controls motor drive convex
Wheel apparatus 10 move, so that whole liquid holding groove 5 is vacillated now to the left, now to the right, so that internal band is cleaned fully.Cleaning one
After the section time, when reaching the time of cleaning dressing, motor control cam makes liquid holding groove be the level of state, now, embedded Control
Device 11 controls the first electromagnetic valve 4 and the first water pump to cut out, and controls the second electromagnetic valve 7 and the second water pump to open simultaneously, will control
TBST waste liquid flows through drain pipe 6, reaches in TBST devil liquor recovery tank 8 until liquid level detecting sensor 16 detects in liquid holding groove no
Cleanout fluid, continues to keep liquid holding groove level, then, embedded controller 11 can control the first electromagnetic valve 4 and the first water pump to open again
Dynamic, TBST cleanout fluid will flow through feed tube 3 from TBST cleanout fluid storage tank 2, reach in liquid holding groove 5, so repeatedly automatic
After cleaning completes the number of times setting, control panel display task completes, and embedded controller 11 can control alarm bell 12 sound simultaneously
Rise and display lamp 13 flashes, remind experimenter.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out with multiple changes in the case of without departing from principle of the present utility model and spirit, repair
Change, replace and modification, scope of the present utility model is defined by the appended claims and the equivalents thereof.
Claims (5)
1. film device is washed, including the first box body by a kind of automatization of Western blot experiment（1）It is characterised in that：Described
First box body（1）Upper end connect the second box body（15）, and the first box body（1）Inner chamber side in be provided with TBST cleanout fluid storage
Tank（2）, and TBST cleanout fluid storage tank（2）By feed tube（3）And liquid holding groove（5）One end insertion connect, liquid holding groove（5）Interior
Containing liquid level detecting sensor（16）, feed tube（3）It is provided with the first electromagnetic valve（4）With the first water pump, described liquid holding groove（5）Pass through
Cylinder（9）With the second box body（15）Rotate and connect, and liquid holding groove（5）With the second box body（15）Between be provided with cam gear
（10）, cam gear（10）It is provided with motor, the bottom of motor is fixedly connected the second box body（15）Upper surface, described Sheng
Liquid bath（5）The other end pass through drain pipe（6）With TBST devil liquor recovery tank（8）Insertion connects, drain pipe（6）It is provided with the second electricity
Magnet valve（7）With the second water pump, and TBST devil liquor recovery tank（8）Positioned at the first box body（1）Opposite side inner chamber in, described first electricity
Magnet valve（4）, the second electromagnetic valve（7）With motor all and embedded controller（11）It is electrically connected with, and embedded controller（11）
Simultaneously with control panel（14）, alarm bell（12）, display lamp（13）And liquid level detecting sensor（16）It is electrically connected with, described embedded
Formula controller（11）, alarm bell（12）And display lamp（13）Positioned at the second box body（15）Inside, control panel（14）It is fixedly connected
In the second box body（15）Lateral wall, liquid level detecting sensor（16）It is disposed vertically in liquid holding groove（5）Interior.
2. a kind of automatization of Western blot experiment according to claim 1 wash film device it is characterised in that：
Described first box body（1）Bottom be fixedly connected four support columns, and the bottom of support column is provided with anti-slip veins.
3. a kind of automatization of Western blot experiment according to claim 1 wash film device it is characterised in that：
Described cam gear（10）Including a major axis and four cams, four cams all fixedly sleeved on major axis.
4. a kind of automatization of Western blot experiment according to claim 1 wash film device it is characterised in that：
Described first box body（1）With the second box body（15）It is rectangular structure, cylinder（9）Positioned at liquid holding groove（5）Bottom center
Position.
5. a kind of automatization of Western blot experiment according to claim 1 wash film device it is characterised in that：
Available control panel（14）It is located at the second box body using by key assignments and liquid crystal display（15）Lateral wall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620944338.7U CN205988933U (en) | 2016-08-26 | 2016-08-26 | Film device is washed by a kind of automatization of Western blot experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620944338.7U CN205988933U (en) | 2016-08-26 | 2016-08-26 | Film device is washed by a kind of automatization of Western blot experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205988933U true CN205988933U (en) | 2017-03-01 |
Family
ID=58107396
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620944338.7U Expired - Fee Related CN205988933U (en) | 2016-08-26 | 2016-08-26 | Film device is washed by a kind of automatization of Western blot experiment |
Country Status (1)
| Country | Link |
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| CN (1) | CN205988933U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108188094A (en) * | 2018-02-05 | 2018-06-22 | 朱宏明 | A kind of automatic Film washers of WB |
| CN108453085A (en) * | 2018-03-30 | 2018-08-28 | 倪华静 | Western Blot intelligently wash film device |
| CN110221057A (en) * | 2019-07-05 | 2019-09-10 | 中国医科大学附属盛京医院 | A kind of automatic washing film device |
| CN110479107A (en) * | 2019-09-09 | 2019-11-22 | 南京医科大学第二附属医院 | A kind of deposited educate of westernblot washes bellows |
| CN110850098A (en) * | 2019-11-21 | 2020-02-28 | 中国人民解放军西部战区总医院 | Automatic membrane washing device for Western blot experiment |
-
2016
- 2016-08-26 CN CN201620944338.7U patent/CN205988933U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108188094A (en) * | 2018-02-05 | 2018-06-22 | 朱宏明 | A kind of automatic Film washers of WB |
| CN108188094B (en) * | 2018-02-05 | 2024-02-23 | 朱宏明 | Automatic film washing machine for western blot test |
| CN108453085A (en) * | 2018-03-30 | 2018-08-28 | 倪华静 | Western Blot intelligently wash film device |
| CN110221057A (en) * | 2019-07-05 | 2019-09-10 | 中国医科大学附属盛京医院 | A kind of automatic washing film device |
| CN110479107A (en) * | 2019-09-09 | 2019-11-22 | 南京医科大学第二附属医院 | A kind of deposited educate of westernblot washes bellows |
| CN110479107B (en) * | 2019-09-09 | 2023-11-24 | 南京医科大学第二附属医院 | Westernblot dressing and washing film box |
| CN110850098A (en) * | 2019-11-21 | 2020-02-28 | 中国人民解放军西部战区总医院 | Automatic membrane washing device for Western blot experiment |
| CN110850098B (en) * | 2019-11-21 | 2023-11-14 | 中国人民解放军西部战区总医院 | Automatic film washing device for Western blot experiment |
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Legal Events
| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170301 Termination date: 20210826 |