CN110221057A - A kind of automatic washing film device - Google Patents
A kind of automatic washing film device Download PDFInfo
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- CN110221057A CN110221057A CN201910601215.1A CN201910601215A CN110221057A CN 110221057 A CN110221057 A CN 110221057A CN 201910601215 A CN201910601215 A CN 201910601215A CN 110221057 A CN110221057 A CN 110221057A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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Abstract
The invention discloses a kind of automatic washing film devices, including washing film device main body, liquid storage box, placing groove, fluid reservoir, outlet tube, the first connection-peg, rotary oscillator, placement tray, gripping component, incubation box, partition, placement lattice, top cover, buckle, liquid injection pipe, the second connection-peg, drain pipe, valve, suction pumps, liquid pumping pipe, the first insertion pipe, pipette, the second insertion pipe and sucker, liquid storage box is provided on the side outer wall for washing film device main body;The invention, structure is relatively simple compact, by connection-peg and insertion pipe be used cooperatively and the use of suction pumps, the automatic feeding fluid for realizing cleaning solution realizes that the later period is incubated for fixed placement of the box in rotary oscillator by gripping the setting of component, the setting of the equipment, which solves traditional TBST and washes in membrane process to change liquid and wash film, needs manual operation, the problem of clamping pvdf membrane repeatedly, easily causing the damage and pollution of film, influence experimental result.
Description
Technical field
The present invention relates to film device technical field is washed, specially a kind of automatic washing film device.
Background technique
Westernblot method is a kind of technology for combining electrophoresis with KIJSA, can be divided into electrophoresis, transfer, enzyme are exempted from
Epidemic disease measures 3 stages;Westernblot method combines the high-resolution of electrophoresis and the hypersensitivity of enzyme immunoassay (EIA) and special
Property, it is a kind of immunological assay method that can be used to analyze sample components;It is essential wherein in westernblot experiment
The step of be milk be incubated for repeatedly TBST wash film, wherein westernblot experiment wash process in film be not correspondingly arranged it is whole
Body washes film device, often needs manually to carry out to change liquid and washes the operation of film, needs to clamp pvdf membrane repeatedly, easily cause the damage of film
With pollution;And pvdf membrane is easily contaminated;Therefore it is necessary for designing a kind of automatic washing film device.
Summary of the invention
The purpose of the present invention is to provide a kind of automatic washing film devices, to solve mentioned above in the background art ask
Topic.
In order to solve the above technical problem, the present invention provides following technical solutions: a kind of automatic washing film device, including washes
Film device main body, liquid storage box, placing groove, fluid reservoir, outlet tube, the first connection-peg, rotary oscillator, placement tray, clamping
Fixation kit is incubated for box, partition, places lattice, top cover, buckle, liquid injection pipe, the second connection-peg, drain pipe, valve, suction pumps, pump
Liquid pipe, the first insertion pipe, pipette, the second insertion pipe and sucker are provided with storage on the side outer wall for washing film device main body
Liquid box is distributed equipped with placing groove on the top outer wall of the liquid storage box, and the inside of the placing groove is placed with fluid reservoir,
Perforation is connected with outlet tube on the top outer wall of the fluid reservoir, and the first grafting is fixedly connected on the top outer wall of outlet tube
Head, it is described wash film device main body and be located on the outer wall of liquid storage box side be provided with rotary oscillator, the rotary oscillator
Placement tray is fixedly connected on the outer wall of top, by gripping component fixed clamp on the top inner wall of the placement tray
There is incubation box;
The component that grips includes mounting blocks, mating groove, spring, movable block, connecting rod and pressure holding plate, the placement
Inlayed on the two sides inner wall of pallet be fixed with mounting blocks, the inside of the mounting blocks offers mating groove, the one of the mating groove
Spring is welded on the inner wall of side, the mating groove, which is located on the inner wall of spring side, is provided with movable block, the movable block
Connecting rod is welded at the outer wall center of side, the outside of one end through the mounting blocks of the connecting rod is fixedly connected with pressure
Plate is held, is separated by being cross-linked partition in placement lattice, the side outer wall for being incubated for box on the top inner wall for being incubated for box
Upper to be connected with top cover by plastic hinge, the top cover is located at perforation on the outer wall placed above lattice and is connected with liquid injection pipe, described
The second connection-peg is fixedly connected on the top outer wall of liquid injection pipe, the box that is incubated for divides on the bottom end outer wall for placing lattice side
Cloth perforation is connected with drain pipe, and the side of the rotary oscillator is provided with suction pumps, and the imbibition end of the suction pumps, which penetrates through, to be connected
It is connected to pipette, the corresponding first connection-peg perforation in one end of the pipette is connected with the second insertion pipe, the pump of the suction pumps
The perforation of liquid end is connected with liquid pumping pipe, and the corresponding second connection-peg perforation in one end of the liquid pumping pipe is connected with the first insertion pipe.
According to the above technical scheme, the lattice and the number of liquid injection pipe placed are eight, and the bottom end of liquid injection pipe is in cone
Shape.
According to the above technical scheme, the side outer wall of the incubation box and top cover is correspondingly arranged on buckle.
According to the above technical scheme, the number of the drain pipe is eight, and fixed peace on the top outer wall of drain pipe
Equipped with valve.
According to the above technical scheme, the pressure holding plate is symmetrical set, and fitting is fixed on the side outer wall of pressure holding plate
There is foam-rubber cushion.
According to the above technical scheme, the inner wall of the movable block and mating groove is the component that is slidably matched, and mating groove is interior
Portion is coated with lubricating oil.
According to the above technical scheme, first insertion pipe is with the first connection-peg with the second connection-peg and the second insertion pipe
Mating component.
According to the above technical scheme, sucker is fixedly connected on the bottom end two sides outer wall of the rotary oscillator.
Compared with prior art, the beneficial effects obtained by the present invention are as follows being: the invention, structure is relatively simple compact, concentrates
Milk is incubated for TBST and washes film device, and automatization level is higher, being used cooperatively and inhale by connection-peg and insertion pipe
The automatic feeding fluid of cleaning solution is realized in the use of liquid pump, the automatic drain in later period is carried out by drain pipe, by gripping component
Setting, realize that the later period is incubated for fixed placement of the box in rotary oscillator, the setting of the equipment solves traditional TBST and washes film
Liquid is changed in the process and washes film and needs manual operation, is clamped pvdf membrane repeatedly, is easily caused the damage and pollution of film, influence experimental result
The problem of.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is entirety perspective view of the invention;
Fig. 2 is the schematic perspective view that the present invention is incubated for box;
Fig. 3 is the schematic perspective view that placement tray of the present invention is placed;
Fig. 4 is the vertical view cutting schematic enlarged-scale view that the present invention grips component;
Fig. 5 is the schematic enlarged-scale view of a-quadrant in Fig. 4 of the present invention;
In figure: 1, washing film device main body;2, liquid storage box;3, placing groove;4, fluid reservoir;5, outlet tube;6, the first grafting
Head;7, rotary oscillator;8, placement tray;9, component is gripped;10, it is incubated for box;11, partition;12, lattice are placed;13, it pushes up
Lid;14, it buckles;15, liquid injection pipe;16, the second connection-peg;17, drain pipe;18, valve;19, suction pumps;20, liquid pumping pipe;21,
First insertion pipe;22, pipette;23, the second insertion pipe;24, sucker;91, mounting blocks;92, mating groove;93, spring;94, it moves
Motion block;95, connecting rod;96, plate is pressed.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1-5 is please referred to, the present invention provides a kind of technical solution: a kind of automatic washing film device, including wash film device master
Body 1, liquid storage box 2, placing groove 3, fluid reservoir 4, outlet tube 5, the first connection-peg 6, rotary oscillator 7, placement tray 8, clamping
Fixation kit 9 is incubated for box 10, partition 11, places lattice 12, top cover 13, buckle 14, liquid injection pipe 15, the second connection-peg 16, drain pipe
17, valve 18, suction pumps 19, liquid pumping pipe 20, the first insertion pipe 21, pipette 22, the second insertion pipe 23 and sucker 24 wash film dress
It sets and is provided with liquid storage box 2 on the side outer wall of main body 1, be distributed on the top outer wall of liquid storage box 2 equipped with placing groove 3, put
The inside for setting slot 3 is placed with fluid reservoir 4, and perforation is connected with outlet tube 5, and the top of outlet tube 5 on the top outer wall of fluid reservoir 4
It is fixedly connected with the first connection-peg 6 on outer wall, washes film device main body 1 and is located on the outer wall of 2 side of liquid storage box and be provided with rotation
Shaker 7 is fixedly connected with placement tray 8 on the top outer wall of rotary oscillator 7, passes through on the top inner wall of placement tray 8
It grips 9 fixing clamp of component and holds incubation box 10;
Gripping component 9 includes mounting blocks 91, mating groove 92, spring 93, movable block 94, connecting rod 95 and pressure holding plate
96, inlay on the two sides inner wall of placement tray 8 be fixed with mounting blocks 91, the inside of mounting blocks 91 offers mating groove 92, cooperation
Spring 93 is welded on the side inner wall of slot 92, mating groove 92, which is located on 93 side inner wall of spring, is provided with movable block 94,
It is welded with connecting rod 95 at the side outer wall center of movable block 94, one end of connecting rod 95 is through the outside of mounting blocks 91
It is fixedly connected with pressure holding plate 96, is incubated on the top inner wall of box 10 and is separated by being cross-linked partition 11 in lattice 12 are placed, is incubated for
Top cover 13 is connected with by plastic hinge on the side outer wall of box 10, top cover 13 is located at the company of perforation on the outer wall for placing 12 top of lattice
It is connected to liquid injection pipe 15, the second connection-peg 16 is fixedly connected on the top outer wall of liquid injection pipe 15, box 10 is incubated for and is located at placement lattice 12
Distribution perforation is connected with drain pipe 17 on the bottom end outer wall of side, and the side of rotary oscillator 7 is provided with suction pumps 19, suction pumps
19 imbibition end perforation is connected with pipette 22, and corresponding first connection-peg 6 perforation in one end of pipette 22 is connected with the second grafting
Pipe 23, the pump liquid end perforation of suction pumps 19 are connected with liquid pumping pipe 20, and corresponding second connection-peg 16 perforation in one end of liquid pumping pipe 20 connects
It is connected to the first insertion pipe 21;The number for placing lattice 12 and liquid injection pipe 15 is eight, and the bottom end of liquid injection pipe 15 is tapered, passes through
Liquid injection pipe 15 carries out being automatically added to for cleaning solution later period;The side outer wall for being incubated for box 10 and top cover 13 is correspondingly arranged on buckle 14;
The fixation for being incubated for 10 upper top cover of box, 13 Gai Hehou is realized by buckle 14;The number of drain pipe 17 is eight, and drain pipe 17
Valve 18 is fixedly installed on the outer wall of top;The discharge for being incubated for 10 cleaning liquid inside of box is controlled by valve 18;Pressing plate 96 is in
It is symmetrical arranged, and fitting is fixed with foam-rubber cushion on the side outer wall of pressure holding plate 96, passes through pressure holding 96 pairs of incubation boxes 10 of plate and carry out
Later period grips;The automatic washing film device in use, first place in the inside of liquid storage box 2 various equipped with cleaning respectively
Fluid reservoir 4 will need the TBST cleaned to wash film difference side into the placement lattice 12 being incubated in box 10, be carried out by top cover 13
Gai He, then the clamping of box 10 will be incubated for and be placed on the placement tray 8 of rotary oscillator 7, then by by 23 grafting of the second insertion pipe
In the first connection-peg 6 on to fluid reservoir 4, then the first insertion pipe 21 is plugged into respectively in the second connection-peg 16, passes through imbibition
Cleaning solution in fluid reservoir 4 is pumped by 19 work of pump respectively to be placed in lattice 12, is worked by rotary oscillator 7 to placement
It is incubated for box 10 on pallet 8 to be vibrated, realizes that being incubated for 10 inside TBST of box carries out washing film, removes after cleaning and is incubated for box 10, passes through
Valve 18 is opened, the cleaning solution in lattice 12 will be placed and be discharged, being performed fully automatic for film is washed in realization, the automatic washing film device, knot
Structure is more compact, is able to achieve and washes the full-automatic of film, solves traditional TBST and washes in membrane process and changes liquid and wash film and need manual operation,
The problem of clamping pvdf membrane repeatedly, easily causing the damage and pollution of film, influence experimental result.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (8)
1. a kind of automatic washing film device, including wash film device main body (1), liquid storage box (2), placing groove (3), fluid reservoir (4),
Outlet tube (5), the first connection-peg (6), rotary oscillator (7), placement tray (8), grip component (9), be incubated for box (10),
Partition (11) places lattice (12), top cover (13), buckle (14), liquid injection pipe (15), the second connection-peg (16), drain pipe (17), valve
Door (18), suction pumps (19), liquid pumping pipe (20), the first insertion pipe (21), pipette (22), the second insertion pipe (23) and sucker
(24), it is characterised in that: be provided with liquid storage box (2) on the side outer wall for washing film device main body (1), the liquid storage box
(2) it is distributed on top outer wall equipped with placing groove (3), the inside of the placing groove (3) is placed with fluid reservoir (4), the storage
Perforation is connected with outlet tube (5) on the top outer wall of flow container (4), and is fixedly connected with first on the top outer wall of outlet tube (5)
Connection-peg (6), it is described wash film device main body (1) and be located on the outer wall of liquid storage box (2) side be provided with rotary oscillator (7),
It is fixedly connected with placement tray (8) on the top outer wall of the rotary oscillator (7), the top inner wall of the placement tray (8)
On by grip component (9) fixing clamp hold be incubated for box (10);
The component (9) that grips includes mounting blocks (91), mating groove (92), spring (93), movable block (94), connecting rod
(95) and pressure holding plate (96), inlay on the two sides inner wall of the placement tray (8) be fixed with mounting blocks (91), the mounting blocks
(91) inside offers mating groove (92), is welded with spring (93) on the side inner wall of the mating groove (92), described
Mating groove (92), which is located on the inner wall of spring (93) side, to be provided with movable block (94), the side outer wall center of the movable block (94)
Place is welded with connecting rod (95), and the outside of one end through the mounting blocks (91) of the connecting rod (95) is fixedly connected with pressure
It holds plate (96), is separated by being cross-linked partition (11) in placement lattice (12) on the top inner wall for being incubated for box (10), it is described
It is incubated on the side outer wall of box (10) and is connected with top cover (13) by plastic hinge, the top cover (13), which is located at, places on lattice (12)
It penetrates through and is connected with liquid injection pipe (15) on the outer wall of side, be fixedly connected with the second connection-peg on the top outer wall of the liquid injection pipe (15)
(16), incubation box (10), which is located on the bottom end outer wall for placing lattice (12) side to be distributed to penetrate through, is connected with drain pipe (17), institute
The side for stating rotary oscillator (7) is provided with suction pumps (19), and the imbibition end perforation of the suction pumps (19) is connected with pipette
(22), one end corresponding first connection-peg (6) perforation of the pipette (22) is connected with the second insertion pipe (23), the suction pumps
(19) pump liquid end perforation is connected with liquid pumping pipe (20), and one end corresponding second connection-peg (16) perforation of the liquid pumping pipe (20) connects
It is connected to the first insertion pipe (21).
2. a kind of automatic washing film device according to claim 1, it is characterised in that: the placement lattice (12) and liquid injection pipe
(15) number is eight, and the bottom end of liquid injection pipe (15) is tapered.
3. a kind of automatic washing film device according to claim 1, it is characterised in that: the incubation box (10) and top cover
(13) side outer wall is correspondingly arranged on buckle (14).
4. a kind of automatic washing film device according to claim 1, it is characterised in that: the number of the drain pipe (17) is
Eight, and valve (18) are fixedly installed on the top outer wall of drain pipe (17).
5. a kind of automatic washing film device according to claim 1, it is characterised in that: the pressure holding plate (96) is in symmetrically to set
It sets, and fitting is fixed with foam-rubber cushion on the side outer wall of pressure holding plate (96).
6. a kind of automatic washing film device according to claim 1, it is characterised in that: the movable block (94) and mating groove
(92) inner wall is the component that is slidably matched, and the inside of mating groove (92) is coated with lubricating oil.
7. a kind of automatic washing film device according to claim 1, it is characterised in that: first insertion pipe (21) and the
Two connection-pegs (16) and the second insertion pipe (23) and the first connection-peg (6) are mating component.
8. a kind of automatic washing film device according to claim 1, it is characterised in that: the bottom of the rotary oscillator (7)
Sucker (24) are fixedly connected on the two sides outer wall of end.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201910601215.1A CN110221057A (en) | 2019-07-05 | 2019-07-05 | A kind of automatic washing film device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201910601215.1A CN110221057A (en) | 2019-07-05 | 2019-07-05 | A kind of automatic washing film device |
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CN110221057A true CN110221057A (en) | 2019-09-10 |
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CN201910601215.1A Pending CN110221057A (en) | 2019-07-05 | 2019-07-05 | A kind of automatic washing film device |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110850098A (en) * | 2019-11-21 | 2020-02-28 | 中国人民解放军西部战区总医院 | Automatic membrane washing device for Western blot experiment |
CN112578111A (en) * | 2020-12-25 | 2021-03-30 | 扬州初心科技服务有限公司 | Food antibiotic wriggling allies oneself with immunity detector more |
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CN205988933U (en) * | 2016-08-26 | 2017-03-01 | 孔德光 | Film device is washed by a kind of automatization of Western blot experiment |
CN206876712U (en) * | 2017-03-13 | 2018-01-12 | 遵义医学院附属医院 | A kind of new Western blot antibody incubation boxes |
CN208438003U (en) * | 2018-06-29 | 2019-01-29 | 时敬龙 | A kind of clamp for numerical control machining of portable Fast Installation |
CN208450048U (en) * | 2018-03-30 | 2019-02-01 | 嘉兴市第二医院 | Intelligence for detected by Western blot washes film device |
-
2019
- 2019-07-05 CN CN201910601215.1A patent/CN110221057A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN205988933U (en) * | 2016-08-26 | 2017-03-01 | 孔德光 | Film device is washed by a kind of automatization of Western blot experiment |
CN206876712U (en) * | 2017-03-13 | 2018-01-12 | 遵义医学院附属医院 | A kind of new Western blot antibody incubation boxes |
CN208450048U (en) * | 2018-03-30 | 2019-02-01 | 嘉兴市第二医院 | Intelligence for detected by Western blot washes film device |
CN208438003U (en) * | 2018-06-29 | 2019-01-29 | 时敬龙 | A kind of clamp for numerical control machining of portable Fast Installation |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110850098A (en) * | 2019-11-21 | 2020-02-28 | 中国人民解放军西部战区总医院 | Automatic membrane washing device for Western blot experiment |
CN110850098B (en) * | 2019-11-21 | 2023-11-14 | 中国人民解放军西部战区总医院 | Automatic film washing device for Western blot experiment |
CN112578111A (en) * | 2020-12-25 | 2021-03-30 | 扬州初心科技服务有限公司 | Food antibiotic wriggling allies oneself with immunity detector more |
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Application publication date: 20190910 |