CN105738575B - Anaerobic ammonium oxidation granular sludge breathing determines devices and methods therefor - Google Patents

Anaerobic ammonium oxidation granular sludge breathing determines devices and methods therefor Download PDF

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Publication number
CN105738575B
CN105738575B CN201610115342.7A CN201610115342A CN105738575B CN 105738575 B CN105738575 B CN 105738575B CN 201610115342 A CN201610115342 A CN 201610115342A CN 105738575 B CN105738575 B CN 105738575B
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cylinder
main
granule sludge
joint
volume
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CN105738575A (en
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郑平
康达
胡倩怡
李旖瑜
叶贞
许少怡
张萌
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Zhejiang University ZJU
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Zhejiang University ZJU
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/28Anaerobic digestion processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials

Abstract

Devices and methods therefor is determined the invention discloses a kind of anaerobic ammonium oxidation granular sludge breathing.Device is integrally divided into main body cylinder, air pressure balancer and the part of gas aspirator three, and main body cylinder is coupled by the U-tube joint and needle tube joint in threaded closure caps with air pressure balancer and gas aspirator.Main body cylinder is provided with threaded closure caps, plenum zone and reaction zone from top to bottom, and reaction zone top is liquid level, and bottom is granule sludge layer, and main body cylinder side wall indicates scale, may indicate that the change of liquid level and granule sludge bed boundary position.Air pressure balancer is covered and liquid bottle provided with minor thread, for main body drum closure and exhaust.Gas aspirator is provided with suction joint, suction drums and pumping piston, for producing gas in negative pressure and release mud.The present invention can quantitative observation granule sludge breathing feature, activity and settleability for indicating granule sludge, simple structure is easy to operate.

Description

Anaerobic ammonium oxidation granular sludge breathing determines devices and methods therefor
Technical field
The invention belongs to field of experiment equipment, and in particular to a kind of anaerobic ammonium oxidation granular sludge breathing determine device and its Method.
Background technology
Anammox be by electron donor of ammonia, nitrous acid be electron acceptor formation nitrogen biological respinse, can be simultaneously Two kinds of pollutants of ammonia and nitrous acid are removed, there is tempting application prospect in wastewater biological denitrificaion.Anaerobic ammonium oxidation granular is dirty Mud is the necessary basis of anaerobic ammonium oxidation sludge bed reactor Effec-tive Function, research and the work of monitoring anaerobic ammonium oxidation granular sludge Property and settleability, help to understand the working condition of anaerobic ammonium oxidation granular sludge in real time, enhancing anaerobic ammonium oxidation sludge bed is anti- Answer the process control and performance optimization of device.
Anaerobic ammonium oxidation granular sludge is made up of anaerobic ammonia oxidizing bacteria, Extracellular polymers (EPS) and hole, matrix (ammonia and Nitrous acid) entered with solubilised state with water inside granule sludge, nitrogen is then changed into by anaerobic ammonia oxidizing bacteria.Nitrogen is in water Solubility is relatively low, is easily separated out in granule sludge and forms bubble;Because the pore size in granule sludge is in capillary yardstick, gas Bubble (nitrogen) easily produces internal pressure by " stifled " due to surface tension in hole, causes granule sludge volumetric expansion.When interior hematocrit When tiring out to more than surface tension, driving bubble is to granule sludge apparent motion, when bubble migration to surface and when discharging, particle dirt Mud internal pressure rapid drawdown, volume contraction produces negative pressure, now sucks water from granule sludge surface again and is dissolved in matrix therein, starts Next round breathing campaign.Because the breathing campaign of anaerobic ammonium oxidation granular sludge is caused by its internal microbial metabolism, and pass through Granule sludge Volume Changes influence the settleability of granule sludge.Therefore, the granule sludge Volume Changes cycle can be may be used to indicate The activity of granule sludge, amplitude of variation may be used to indicate settling of granular sludge.But still set at present without a kind of effectively experiment It is standby the breathing situation of anaerobic ammonium oxidation granular sludge effectively to be determined, it is unfavorable for grinding for anaerobic ammonium oxidation granular sludge Study carefully work development.
The content of the invention
Present invention aim to address problems of the prior art, and it is swollen to provide a kind of anaerobic ammonium oxidation granular sludge Contracting determines device, by observing granule sludge volume dilational, indicates the activity and settleability of granule sludge.
To achieve these goals, the concrete technical scheme that the present invention is used is as follows:
A kind of anaerobic ammonium oxidation granular sludge breathing determines device, device be integrally divided into main body cylinder, air pressure balancer and The part of gas aspirator three, specifically includes plenum zone, reaction zone, liquid level, granule sludge layer, major thread capping, the first U Shape pipe joint, needle tube joint, U-tube, the second U-tube joint, liquid bottle, hydroecium, head space room, minor thread capping, blast pipe, row Air valve, suction joint, suction drums, pumping piston;
Main body barrel top is covered provided with major thread, and inside is followed successively by plenum zone and reaction zone from top to bottom;Reaction Area top is liquid level, and bottom is granule sludge layer;Plenum zone be a head space, for collect reaction zone generation gas with And the negative pressure that transmission gas aspirator is produced;Main body cylinder side wall indicates scale, may indicate that liquid level and granule sludge bed boundary The change of position;Major thread capping is provided with U-tube joint and needle tube joint, and U-tube joint passes through U-tube and air pressure balancer UNICOM, needle tube joint is by aspirating joint and gas aspirator UNICOM;
Air pressure balancer is made up of minor thread capping and liquid bottle, is divided into head space room and water inside liquid bottle from top to bottom Room;Minor thread capping is provided with U-tube joint and blast pipe, and U-tube joint passes through U-tube and main body cylinder UNICOM, blast pipe Top is with atmosphere and provided with air bleeding valve;
Gas aspirator is provided with suction joint, suction drums and pumping piston;Joint is aspirated joint, bottom and pin is sanded Pipe seal is coupled;Suction drums side wall indicates scale, for indicating Volume Changes;Pumping piston is placed in suction drums, Seal but can twitch with side wall.
Preferably, the ratio between reaction zone and plenum zone volume are 4 in described main body cylinder:1;Liquid in reaction zone The ratio between layer and granule sludge layer volume are 1:2.
Preferably, the ratio between reaction zone volume is 1 in liquid bottle and main body cylinder in described air pressure balancer:9;Fill liquid The ratio between head space room and hydroecium volume are 1 in bottle:4;U-tube joint stretches into 20~30mm below the hydroecium water surface, for air-isolation.
Preferably, the ratio between plenum zone volume is 2 in suction drums and main body cylinder in described gas aspirator: 1。
Another object of the present invention is to provide a kind of anaerobic ammonium oxidation granular sludge breathing measure of use described device Method, step is as follows:
First nutrient solution is put into the reaction zone of main body cylinder, record fluid level scale H1;It is again that anaerobic ammonium oxidation granular is dirty Mud continues to be put into reaction zone, record fluid level scale H2;By the difference H of two liquid levels2-H1It is used as granule sludge initial volume;Use big spiral shell Line lidstock main body cylinder, opens the air bleeding valve of air pressure balancer, pumping piston in gas aspirator is pushed into zero graduation;It is right Anaerobic ammonium oxidation granular sludge is cultivated, fluid level scale in main body cylinder when hydroecium no longer produces bubble in record liquid bottle H3, the difference H of fluid level scale when main body cylinder fluid level scale and granule sludge now have just been put into main body cylinder3-H2It is dirty for particle Mud swelling volume;The air bleeding valve of air pressure balancer is closed, the gas in main body cylinder is pumped out with gas aspirator, justifies main body The main body cylinder fluid level scale H after negative pressure, granule sludge discharge bubble, record granule sludge discharge bubble is produced in cylinder4;Now Fluid level scale when being just put into main body cylinder of main body cylinder fluid level scale and granule sludge difference H2-H4For granule sludge shrinkage Volume.
Further, drift along situation and volume dilational change of the association granule sludge in main body cylinder I, for quantitative scoring Calculate the bubble volume allowed in anaerobic ammonium oxidation granular sludge.
The beneficial effects of the invention are as follows:1) device simple structure, it is easy to operate;2) gas aspirator and air pressure balance are passed through Device is used cooperatively, the micro volume change of Observable granule sludge breathing;3), can by the period of change of granule sludge volume Judge granule sludge activity;4) by the amplitude of variation of granule sludge volume, it can determine whether settling of granular sludge.
Brief description of the drawings
Fig. 1 is a kind of anaerobic ammonium oxidation granular sludge breathing assay device structures figure;
In figure:Main body cylinder I, air pressure balancer II, gas aspirator III;Plenum zone 1, reaction zone 2, liquid level 3rd, granule sludge layer 4, major thread cover the 5, first U-tube joint 6, needle tube joint 7, U-tube 8, the second U-tube joint 9, dress liquid Bottle 10, hydroecium 11, head space room 12, minor thread cover 13, blast pipe 14, air bleeding valve 15, suction joint 16, suction drums 17 and taken out Attraction piston 18.
Embodiment
With reference to specific the drawings and specific embodiments, the present invention will be further described.If without specified otherwise or punching Prominent, each preferred embodiment can be in any combination.
As shown in figure 1, a kind of anaerobic ammonium oxidation granular sludge breathing determines device.Device is integrally divided into main body cylinder I, gas Pressure balancer II and the parts of gas aspirator III tri-.Specifically, including plenum zone 1, reaction zone 2, liquid level 3, particle Sludge blanket 4, major thread cover the 5, first U-tube joint 6, needle tube joint 7, U-tube 8, the second U-tube joint 9, liquid bottle 10, Hydroecium 11, head space room 12, minor thread capping 13, blast pipe 14, air bleeding valve 15, suction joint 16, suction drums 17, pumping piston 18。
Main body cylinder I is used to place mud granule, and its top is sealed provided with dismountable major thread capping 5.In it Portion space is followed successively by plenum zone 1 and reaction zone 2 from top to bottom.Under use state, the top of reaction zone 2 is liquid level 3, bottom For granule sludge layer 4.Plenum zone 1 is a head space, gas and transmission gas aspirator for collecting the generation of reaction zone 2 The negative pressure that III is produced.Main body cylinder I sides wall indicates scale, may indicate that the change of liquid level and granule sludge bed boundary position.Greatly U-tube joint 6 and needle tube joint 7 of the threaded closure caps 5 provided with outside in connection main body cylinder, U-tube joint 6 pass through U-tube 8 With air pressure balancer II UNICOMs, needle tube joint 7 is by aspirating joint 16 and gas aspirator III UNICOMs.
Air pressure balancer II is made up of minor thread capping 13 and liquid bottle 10, is divided into head space from top to bottom inside liquid bottle 10 Room 12 and hydroecium 11;Minor thread capping 13 is provided with U-tube joint 9 and blast pipe 14, and U-tube joint 9 passes through U-tube 8 and master Body cylinder I UNICOMs, the top of blast pipe 14 is with atmosphere and provided with air bleeding valve 15.
Gas aspirator III uses the design of similar syringe, provided with suction joint 16, suction drums 17 and pumping piston 18.Joint 16 is aspirated joint, bottom and the sealed connection of needle tube joint 7 is sanded;The side wall of suction drums 17 indicates scale, for referring to Show Volume Changes.Pumping piston 18 is placed in suction drums 17, with side wall sealing but can piston type twitch.
The size and the visual actual conditions of ratio of above-mentioned each device are set.In the present embodiment, described main body The ratio between reaction zone 2 and the volume of plenum zone 1 are 4 in cylinder I:1;In reaction zone 24 volume of liquid level 3 and granule sludge layer it Than for 1:2.The ratio between volume of reaction zone 2 is 1 in liquid bottle 10 and main body cylinder I in described air pressure balancer II:9;Liquid bottle The ratio between head space room 12 and the volume of hydroecium 11 are 1 in 10:4;U-tube joint 9 stretches into 20~30mm below the water surface of hydroecium 11, for every From air.The ratio between volume of plenum zone 1 is 2 in suction drums 17 and main body cylinder I in described gas aspirator III:1. Through overtesting, above-mentioned size and ratio can preferably complete the test purpose of the present invention.
Aerogenesis and situation of drifting along for the ease of Real Time Observation mud granule, described main body cylinder I are had using transparent Machine glass makes;Described air pressure balance II can use Clear glass bottles and jars.
Based on said apparatus, present invention also offers a kind of anaerobic ammonium oxidation granular sludge breathing assay method, step is such as Under:
First nutrient solution is put into main body cylinder I reaction zone 2, record fluid level scale H1;Again by anaerobic ammonium oxidation granular Sludge continues to be put into reaction zone 2, record fluid level scale H2;By the difference H of two liquid levels2-H1It is used as granule sludge initial volume;With Major thread covers 5 sealed body cylinder I, air pressure balancer II air bleeding valve 15 is opened, by pumping piston in gas aspirator III 18 are pushed into zero graduation.After anaerobic ammonium oxidation granular sludge culture for a period of time, hydroecium in air pressure balancer liquid bottle can be observed Bubble is produced, granule sludge activity size can be qualitatively judged according to bubble severe degree.In the training of anaerobic ammonium oxidation granular sludge During supporting, fluid level scale H in main body cylinder I when hydroecium 11 no longer produces bubble in record liquid bottle 103, main body now The difference H of fluid level scale when cylinder fluid level scale and granule sludge have just been put into main body cylinder3-H2For granule sludge swelling volume;Close Hold one's breath pressure balancer II air bleeding valve 15, pump out the gas in main body cylinder I with gas aspirator III, make in main body cylinder Produce the main body cylinder fluid level scale H after negative pressure, granule sludge discharge bubble, record granule sludge discharge bubble4;Master now The difference H of fluid level scale when body cylinder I fluid level scales and granule sludge have just been put into main body cylinder2-H4For granule sludge shrinkage body Product.
In addition, in continuous mode, if drift along situation and volume dilational change of the association granule sludge in main body cylinder I, The bubble volume allowed for quantitatively calculating in anaerobic ammonium oxidation granular sludge.Meanwhile, pass through the change week of granule sludge volume Phase, it can determine whether granule sludge activity;By the amplitude of variation of granule sludge volume, settling of granular sludge can determine whether.Therefore, originally Invention can quantitative observation granule sludge breathing feature, activity and settleability for indicating granule sludge, and with construction letter Single, easy to operate advantage.

Claims (6)

1. a kind of anaerobic ammonium oxidation granular sludge breathing determines device, it is characterised in that:Device is integrally divided into main body cylinder(I)、 Air pressure balancer(II)With gas aspirator(III)Three parts, specifically include plenum zone(1), reaction zone(2), liquid level (3), granule sludge layer(4), major thread capping(5), the first U-tube joint(6), needle tube joint(7), U-tube(8), the second U-shaped Pipe joint(9), liquid bottle(10), hydroecium(11), head space room(12), minor thread capping(13), blast pipe(14), air bleeding valve (15), suction joint(16), suction drums(17), pumping piston(18);
Main body cylinder(I)Top is covered provided with major thread(5), inside is followed successively by plenum zone from top to bottom(1)And reaction zone (2);Reaction zone(2)Top is liquid level(3), bottom is granule sludge layer(4);Plenum zone(1)For a head space, for receiving Collect reaction zone(2)The gas and transmission gas aspirator of generation(III)The negative pressure of generation;Main body cylinder(I)Side wall indicates quarter Degree, may indicate that the change of liquid level and granule sludge bed boundary position;Major thread is covered(5)It is provided with the first U-tube joint(6) And needle tube joint(7), the first U-tube joint(6)Pass through U-tube(8)With air pressure balancer(II)UNICOM, needle tube joint(7)It is logical Cross suction joint(16)With gas aspirator(III)UNICOM;
Air pressure balancer(II)Covered by minor thread(13)And liquid bottle(10)Composition, liquid bottle(10)Inside is divided into from top to bottom Head space room(12)And hydroecium(11);Minor thread is covered(13)It is provided with the second U-tube joint(9)And blast pipe(14), the second U-shaped Pipe joint(9)Pass through U-tube(8)With main body cylinder(I)UNICOM, blast pipe(14)Top is with atmosphere and provided with air bleeding valve (15);
Gas aspirator(III)Provided with suction joint(16), suction drums(17)And pumping piston(18);Aspirate joint(16)For Joint, bottom and needle tube joint is sanded(7)Sealed connection;Suction drums(17)Side wall indicates scale, for indicating Volume Changes; Pumping piston(18)It is placed in suction drums(17)It is interior, seal but can twitch with side wall.
2. anaerobic ammonium oxidation granular sludge breathing according to claim 1 determines device, it is characterised in that:Described main body Cylinder(I)Interior reaction zone(2)And plenum zone(1)The ratio between volume is 4:1;Reaction zone(2)Interior liquid level(3)With granule sludge Layer(4)The ratio between volume is 1:2.
3. anaerobic ammonium oxidation granular sludge breathing according to claim 1 determines device, it is characterised in that:Described air pressure Balancer(II)Interior liquid bottle(10)With main body cylinder(I)Interior reaction zone(2)The ratio between volume is 1:9;Liquid bottle(10)Interior head space Room(12)And hydroecium(11)The ratio between volume is 1:4;Second U-tube joint(9)Stretch into hydroecium(11)20~30mm below the water surface, is used In air-isolation.
4. anaerobic ammonium oxidation granular sludge breathing according to claim 1 determines device, it is characterised in that:Described gas Aspirator(III)Interior suction drums(17)With main body cylinder(I)Interior plenum zone(1)The ratio between volume is 2:1.
5. a kind of usage right requires the anaerobic ammonium oxidation granular sludge breathing assay method of 1 described device, it is characterised in that step It is rapid as follows:
Nutrient solution is first put into main body cylinder(I)Reaction zone(2)It is interior, record fluid level scale H1;It is again that anaerobic ammonium oxidation granular is dirty Mud continues to be put into reaction zone(2)It is interior, record fluid level scale H2;By the difference H of two liquid levels2-H1It is used as granule sludge initial volume;With Major thread is covered(5)Sealed body cylinder(I), open air pressure balancer(II)Air bleeding valve(15), by gas aspirator(III) Interior pumping piston(18)It is pushed into zero graduation;Anaerobic ammonium oxidation granular sludge is cultivated, liquid bottle is recorded(10)Interior hydroecium (11)No longer produce main body cylinder during bubble(I)Middle fluid level scale H3, main body cylinder fluid level scale and granule sludge now The difference H of fluid level scale when being just put into main body cylinder3-H2For granule sludge swelling volume;Close air pressure balancer(II)Exhaust Valve(15), use gas aspirator(III)Pump out main body cylinder(I)Interior gas, makes to produce negative pressure in main body cylinder, particle is dirty Mud discharges the main body cylinder fluid level scale H after bubble, record granule sludge discharge bubble4;Main body cylinder now(I)Liquid level is carved The difference H of fluid level scale when degree and granule sludge have just been put into main body cylinder2-H4For granule sludge shrinkage volume.
6. anaerobic ammonium oxidation granular sludge breathing assay method as claimed in claim 5, it is characterised in that association granule sludge In main body cylinder(I)Interior drift along situation and volume dilational change, allows for quantitatively calculating in anaerobic ammonium oxidation granular sludge Bubble volume.
CN201610115342.7A 2016-03-01 2016-03-01 Anaerobic ammonium oxidation granular sludge breathing determines devices and methods therefor Active CN105738575B (en)

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CN106248915B (en) * 2016-08-13 2018-06-19 中交一公局桥隧工程有限公司 Engineered cementitious concrete self-shrinkage measuring method and measuring device
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KR20110068697A (en) * 2009-12-16 2011-06-22 설동화 Sludge bulking improvement agent for biological treatment of sewage or waste water, and method for water treatment using the same
CN101863554B (en) * 2010-06-04 2012-03-28 浙江大学 Superhigh-efficiency anammox reactor
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CN204058059U (en) * 2014-07-17 2014-12-31 浙江大学 The bio-reactor of a kind of synchronous removal nitre nitrogen and ammonia nitrogen
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