CN117491113A - Full-automatic intelligent slide glass dyeing equipment - Google Patents

Abstract

The invention provides full-automatic intelligent slide glass dyeing equipment, which comprises a rack, wherein the rack is provided with a plurality of slide glass dyeing units; an automatic sampling mechanism mounted on the frame; the reagent classification storage mechanism is arranged on the rack and positioned below the automatic sampling mechanism; the glass slide shaking mechanism comprises a bottom plate arranged on a frame, a water bath mechanism is arranged on the bottom plate, a protective shell is arranged on the outer side of the water bath mechanism, and a glass slide loading shaking mechanism capable of turning and shaking is arranged above the water bath mechanism on the protective shell. This full-automatic intelligent slide glass dyeing equipment is automatic, intelligent degree is high, through the structural design to dyeing equipment, can increase the quantity of placing of slide glass by a wide margin to slide glass after the dropwise add staining solution is inclined shakes, improves the dyeing effect, and can carry out classified storage to different types of staining solution, improves application of sample efficiency.

Description

Full-automatic intelligent slide glass dyeing equipment

Technical Field

The invention relates to the technical field of dyeing equipment, in particular to full-automatic intelligent slide glass dyeing equipment.

Background

Biological slide is the basic method of studying the microstructure of biological organisms, and the aim is to provide a sample that is reasonably microscopic and effectively examined for morphology and function. In the course of performing an experimental or medical examination on a specimen, it is necessary to observe cells or tissue sections on a slide by a microscope, and it is necessary to stain the cells or tissue sections on the slide before that.

The slide glass dyeing machine is equipment capable of realizing automatic sample loading and dyeing, for example, chinese patent publication No. CN210690188U discloses a pathology dyeing machine, which comprises a reagent storage unit, an incubation device, an atomization device, a first manipulator and a multi-axis movement mechanism, wherein the reagent storage unit is used for placing a reagent; the incubation device is provided with an incubation cavity, the Wen Yoqiang is suitable for accommodating a glass slide and heating and preserving the glass slide; an atomizing device in communication with said Wen Yoqiang for providing atomized droplets to said incubation cavity to maintain humidity within said Wen Yoqiang; the first manipulator comprises a sample adding needle and a first driving mechanism for driving the sample adding needle to move up and down, and is used for adding the reagent onto the glass slide through the sample adding needle; the multi-axis movement mechanism is connected with the first manipulator and used for driving the first manipulator to move. The pathology dyeing machine can realize automatic dyeing operation, has high operation consistency, reliable and stable dyeing effect and good consistency, and has higher dyeing efficiency. However, in the practical use process, since the glass slide of the pathology dyeing machine is horizontally tiled, the occupied space is large, multiple dyeing liquids are needed to be sequentially used in the actual dyeing process, multiple dyeing needs to be subjected to mixed reaction for a period of time, if the mixed reaction is not interfered, the mixed reaction is completely dependent on the fusion of the multiple dyeing liquids, the reaction time is prolonged, and the whole dyeing efficiency is low.

To current well slide glass generally can only adopt horizontal tiling to place, lead to occupation space big, the patent CN213434560U of this application prior application discloses a firm slide glass erects and puts the support (its structure is as shown in figure 1) to the patent CN216051028U of another prior application of cooperation applicant, a novel slide glass fixed knot constructs (its structure is as shown in figure 2), can realize erecting of slide glass and place, on same cross-sectional area, can improve the quantity that the slide glass was placed by a wide margin, improves the efficiency of dyeing, but the slide glass of vertical placement is after dropwise adding multiple staining solution in proper order, if do not slope shake, the effect of dyeing can become worse.

In addition, in the actual staining process, a plurality of staining solutions are required to be sequentially used, and different kinds of staining solutions are gradually added to the glass slide by a pipette to stain the tissues on the glass slide. In the actual sample adding process, a manipulator is required to accurately move the pipettor to different reagent boxes, and the staining solution in the reagent boxes is sucked and transferred. In the prior art, generally, only one dyeing liquid can be sucked by a control manipulator at a time, then the control manipulator moves to a glass slide for dyeing, then after needle replacement, the control manipulator moves to another dyeing liquid for sucking, and the overall efficiency is low. Two or more staining solutions cannot be sucked at a time on the premise of not changing the needle, and the staining solution can be moved to the glass slide for staining at a time. If a plurality of manipulators are provided, the overall volume of the apparatus increases, and the cost of the apparatus increases.

In addition, as a plurality of kinds of staining solutions are needed to be used in sequence in the actual staining process, but the storage environment requirements of different kinds of staining solutions are different, how to store different kinds of staining solutions in a classified manner and facilitate the automatic suction and replacement of the pipette tip are technical problems to be solved in the field.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides the full-automatic intelligent slide glass dyeing equipment, the placement quantity of slide glass can be greatly increased through the structural design of the dyeing equipment, the slide glass after the dropping of the dyeing liquid is subjected to tilting shaking, the dyeing effect is improved, the different types of dyeing liquids can be classified and stored, and the sample adding efficiency is improved.

In order to achieve the technical scheme, the invention provides full-automatic intelligent slide glass dyeing equipment, which comprises: a frame; an automatic sampling mechanism mounted on the frame; the reagent classifying and storing mechanism is arranged on the rack and positioned below the automatic sampling mechanism and comprises a storing rack, a storing cabinet is arranged on the storing rack, a refrigerating storage box and a normal temperature storage box are arranged in the storing cabinet, the refrigerating storage box comprises a refrigerating box body, a plurality of sliding chute type reagent racks which are arranged in parallel are arranged in the refrigerating box body, the sliding chute type reagent racks comprise a reagent rack body, a plurality of reagent box placing grooves which are distributed at intervals in parallel are arranged on the reagent rack body, a reagent box for loading refrigerating reagent is embedded in the reagent box placing grooves, a sliding block protrusion protruding outwards is arranged at the bottom of the reagent rack body and is embedded in a sliding chute arranged on the refrigerating box body, a sliding door which is convenient for taking the reagent rack body is arranged at the front side of the refrigerating box body, and a plurality of types of reagent storing grooves are arranged in the normal temperature storage box; install slide glass shake mechanism in reagent classified storage mechanism one side, slide glass shake mechanism including installing the bottom plate in the frame, install water bath mechanism on the bottom plate, the shell is installed in the water bath mechanism outside, but the slide glass that lies in water bath mechanism on the shell is loaded and shake mechanism that shakes of overturning is installed to the top that lies in water bath mechanism, slide glass is loaded and shake mechanism includes the pivot, the pivot is along transversely running through installing on the shell, pivot and shell butt joint department all install the bearing, shake servo motor and install on the shell and be connected with the one end of pivot through the shaft coupling, install the loading frame in the pivot, the loading frame is located the top of water bath mechanism, the loading frame separates into a plurality of channels that are used for loading slide glass to erect the support through the partition strip that many parallel intervals set up, and the rear side of every passageway all corresponds and is provided with the location baffle.

Preferably, the water bath mechanism comprises a water bath tank, the water bath tank is fixedly arranged on the bottom plate, a heater is arranged in the water bath tank, a plurality of inductor reserved mounting holes are formed in the top end edge of the water bath tank, an inductor is arranged in each inductor reserved mounting hole, and a positioning mounting groove used for being matched with the positioning embedding of the shell is formed in the top end edge of the water bath tank.

Preferably, the automatic sample adding mechanism comprises a sample adding rack arranged on the rack, an X-direction guide rail is arranged at the top of the sample adding rack along the X-direction, an X-direction linear module is arranged on the X-direction guide rail, a movable sample adding device is arranged at the bottom of the X-direction linear module and comprises a shell, the top of the shell is fixed at the bottom of the X-direction linear module, a sample adding assembly is arranged in the shell, the sample adding assembly comprises a mounting rack fixed at the inner side of the shell, Y-direction guide rails are arranged on the mounting rack along the Y-direction, a plurality of sample adding mechanisms which are independently controlled are arranged on the Y-direction guide rails, the structures of the sample adding mechanisms are the same, each sample adding mechanism comprises a Z-direction support, the Z-direction support is arranged on the Y-direction guide rail through a Y-direction moving seat, a Y-direction linear module for driving the Z-direction support to move along the Y-direction guide rail is arranged on the Y-direction linear module, the Z-direction moving seat is arranged on the Z-direction guide rail, the Z-direction linear module is arranged on the Z-direction guide rail and is used for driving the Z-direction linear module to move along the Z-direction seat to move along the Z-direction guide rail, the Z-direction seat is arranged on the Z-direction guide seat vertically, and the Z-direction seat is arranged on the liquid guide seat.

Preferably, the end of the liquid transfer device is provided with a needle head joint, and a buffer spring is arranged at the butt joint part of the needle head joint and the end of the liquid transfer device.

Preferably, the X-direction linear module, the Y-direction linear module and the Z-direction linear module have the same structure and all comprise screw rods distributed along the direction of the guide rail, screw rod pairs are arranged on the screw rods, sliding blocks are arranged on the screw rod pairs, and a servo motor is arranged on one side of the screw rods and connected with the screw rods.

Preferably, the top of the shell of the movable sample adding device is fixed at the bottom of the sliding block in the X-direction linear module, the Y-direction movable seat is fixed on the sliding block of the Y-direction linear module, and the Z-direction movable seat is fixed on the sliding block of the Z-direction linear module.

Preferably, the refrigerator body comprises a box body, a refrigerator is arranged at the bottom of the box body, an outer cover is arranged at the outer side of the refrigerator, a plurality of sliding grooves which are arranged at intervals in parallel are arranged in the box body, and sliding blocks arranged at the bottom of the reagent frame body are embedded in the sliding grooves.

Preferably, two parallel open-type reagent storage tanks, two parallel partition-type reagent storage tanks and four parallel closed-type reagent storage tanks are arranged in the normal-temperature storage tank, and a needle insertion port is formed in the top of each closed-type reagent storage tank.

Preferably, an exhaust needle head collecting groove is arranged at the outer side of the normal temperature storage box, and a needle head unhooking is arranged in the exhaust needle head collecting groove.

Preferably, the outer cover is arranged on the outer side of the automatic sampling mechanism, the reagent classification storage mechanism and the glass slide shaking mechanism on the frame, a front door is arranged on the front side of the outer cover, a transparent observation window is arranged on the front door, and a plurality of moving wheels are arranged at the bottom of the frame.

The full-automatic intelligent slide glass dyeing equipment provided by the invention has the beneficial effects that:

(1) This full-automatic intelligent slide glass dyeing equipment is automatic, intelligent degree is high, through the structural design to dyeing equipment, can increase the quantity of placing of slide glass by a wide margin to slide glass after the dropwise add staining solution is inclined shakes, improves the dyeing effect, and can carry out classified storage to different types of staining solution, improves application of sample efficiency.

(2) This full-automatic intelligent slide glass dyeing equipment shakes structural design of mechanism through shaking the slide glass, can increase the quantity of placing of slide glass by a wide margin, improves dyeing efficiency. When the device works, the vertical slide rack is placed on the slide vertical rack placing channel arranged on the loading rack, and is positioned and fixed through the positioning partition plate, and then the vertical dyed slide is placed on the slide vertical rack. After the slide glass dyeing machine works, a plurality of dyeing liquids are sequentially dripped into the vertically placed slide glass, hot water is filled in the water bath mechanism, the servo motor drives the rotating shaft to drive the loading frame to turn upwards for a certain angle at intervals, the slide glass is taken out of the water bath mechanism, and then the servo motor periodically drives the rotating shaft to drive the loading frame to rotate back and forth in a certain angle at a certain frequency, so that shaking of the slide glass placed on the loading frame is realized, mixing among different dyeing liquids is accelerated, the dyeing effect can be enhanced, and the dyeing efficiency is greatly improved.

(3) This full-automatic intelligent slide glass dyeing equipment can realize the accurate removal of a plurality of pipettes in X to, Y to and Z through the structural design to automatic application of sample mechanism. During actual operation, the whole sample feeding device can be driven to move accurately in the X direction through the X-direction linear module, after the sample feeding device moves in place in the X direction, the Y-direction linear module arranged on each sample feeding mechanism can control the independent movement of a plurality of sample feeding mechanisms arranged on the Y-direction guide rail in the Y direction, and each sample feeding mechanism can independently absorb the same or different staining solutions, so that on the premise of not changing needles, a plurality of staining solutions can be absorbed at one time, and the sample feeding mechanism can be moved to a glass slide for staining at one time, thereby greatly increasing sample feeding efficiency. After the sample adding mechanism moves to the upper part of the corresponding staining solution in the Y direction, the Z-direction linear module drives the liquid transfer device to move up and down along Z so as to control the liquid transfer device to suck and reset the staining solution. After a plurality of pipettors absorb corresponding staining solution, the X-direction linear module drives the movable sample adding device to integrally move to the glass slide for sample adding, so that the sample adding efficiency can be greatly improved.

(4) This full-automatic intelligent slide glass dyeing equipment can carry out classified storage to different types of staining solution through the structural design to reagent classified storage mechanism to make things convenient for the automation of pipetting gun syringe needle to absorb and change. During actual operation, only need place the dye liquor that needs cold-stored processing on the spout formula reagent frame of cold-stored storage box through the kit and refrigerate, and through the structural design to spout formula reagent frame, spout formula reagent frame is protruding through the cooperation of slider with the spout, wholly can follow cold-stored storage box and take out or insert fast, can make things convenient for the quick overall change of a plurality of kits on the spout formula reagent frame to reach the purpose that strengthens kit change efficiency. The staining solution which can be stored at normal temperature can be added into a plurality of types of reagent storage tanks arranged in the normal temperature storage tank for classified storage. In the actual dyeing process, the needle of the liquid-transfering gun can absorb corresponding dyeing reagent from the reagent box in the cold storage box or the reagent storage tank of the normal temperature storage box according to the requirement, gradually add the reagent into the glass slide, dye tissues on the glass slide, when absorbing different dyeing reagents, the liquid-transfering gun needs to move to the waste gas needle collecting tank, the needle is removed through the needle unhooking arranged in the waste gas needle collecting tank, and then the liquid-transfering gun moves to the needle replacing position to replace the needle, so as to avoid cross contamination among different dyeing reagents.

Drawings

Fig. 1 is a schematic view of a prior art slide rack.

Fig. 2 is a schematic diagram of a prior art stained slide.

Fig. 3 is a schematic perspective view of the present invention.

Fig. 4 is an installation schematic diagram of the internal structure of the present invention.

FIG. 5 is a schematic perspective view of an automatic sample loading mechanism according to the present invention.

FIG. 6 is a schematic perspective view of a mobile sample application device according to the present invention.

FIG. 7 is a schematic perspective view of the sample loading assembly of the present invention.

FIG. 8 is a schematic perspective view of the sample loading mechanism of the present invention.

Fig. 9 is a schematic perspective view of a linear module according to the present invention.

FIG. 10 is a schematic perspective view of a reagent sorting and storing mechanism according to the present invention.

Fig. 11 is a layout view of the cold storage box and the normal temperature storage box in the present invention.

Fig. 12 is an exploded perspective view of the cooling storage box of the present invention.

Fig. 13 is a schematic structural installation view of the normal temperature storage box in the present invention.

Fig. 14 is a schematic perspective view of a slide shaking mechanism according to the present invention.

Fig. 15 is a schematic view showing the internal structure of the slide shaking mechanism according to the present invention.

In the figure: 1. a frame; 11. a storage cabinet; 12. an outer cover; 13. a front door; 14. an observation window; 15. and (5) moving the wheel.

2. An automatic sample adding mechanism; 21. a sample adding rack; 22. an X-direction guide rail; 23. an X-direction linear module; 231. a servo motor; 232. a screw rod; 233. a screw pair; 234. a slide block; 24. moving the sample adding device; 241. a housing; 242. a sample application assembly; 2421. a mounting frame; 2422. a Y-direction guide rail; 2423. a sample adding mechanism; 24231. a Z-direction bracket; 24232. y-direction moving seat; 24233. a Y-direction linear module; 24234. a Z-direction guide rail; 24235. a Z-direction moving seat; 24236. a Z-direction linear module; 24237. a pipette mount; 24238. a pipette; 24239. a buffer spring; 242310, needle hub; 243. and the heat dissipation holes.

3. A reagent classification storage mechanism; 31. a storage rack; 32. a sliding door; 33. a storage cabinet; 34. a refrigerated storage case; 341. a refrigerator body; 3411. a case; 3412. a chute; 3413. a refrigerator; 3414. an outer cover; 342. a chute type reagent rack; 3421. a reagent rack body; 3422. a kit placement groove; 3423. a handle; 3424. the sliding block is convex; 343. a kit; 35. an open-type reagent storage tank; 36. partition type reagent storage tank; 37. a closed reagent storage tank; 371. a needle insertion opening; 38. a waste gas needle collection tank; 39. the needle is unhooked.

4. A slide shaking mechanism; 41. a bottom plate; 42. a water bath mechanism; 421. a water bath; 422. a heater; 423. reserving a mounting hole for the inductor; 424. positioning the mounting groove; 43. a protective housing; 44. a slide loading shaking mechanism; 441. a loading rack; 442. a partition strip; 443. positioning a baffle; 444. a rotating shaft; 445. a bearing; 45. a shaking servo motor; 100. a glass slide vertically-arranged bracket; 200. the slides were stained.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments obtained by those skilled in the art without making any inventive effort are within the scope of the present invention.

Examples: a full-automatic intelligent slide glass dyeing device.

Referring to fig. 1 to 15, a fully automatic intelligent slide staining apparatus comprises:

a frame 1;

the automatic sampling mechanism 2 is arranged on the frame 1, the sampling mechanism 2 comprises a sampling frame 21, an X-direction guide rail 22 is arranged at the top of the sampling frame 21 along the X-direction, an X-direction linear module 23 is arranged on the X-direction guide rail 22, a movable sampling device 24 is arranged at the bottom of the X-direction linear module 23, and in actual operation, the movable sampling device 24 can be driven by the X-direction linear module 23 to accurately move on the X-direction guide rail 22.

The movable sample loading device 24 comprises a housing 241, the top of the housing 241 is fixed at the bottom of a sliding block 234 of the X-direction linear module 23, a sample loading assembly 242 is installed in the housing 241, a heat dissipation hole 243 is further formed in the housing 241, the sample loading assembly 242 comprises a mounting rack 2421 fixed at the inner side of the housing 241, a Y-direction guide rail 2422 is arranged on the mounting rack 2421 along the Y-direction, two sample loading mechanisms 2423 which are independently controlled are installed on the Y-direction guide rail 2422, the two sample loading mechanisms 2423 have the same structure and comprise a Z-direction bracket 24231, the Z-direction bracket 24231 is installed on the Y-direction guide rail 2422 through a Y-direction moving seat 24232, a Y-direction linear module 24233 for driving the Z-direction bracket 24231 to move along the Y-direction guide rail 2422 is installed on the Z-direction guide rail 24231, a Z-direction moving seat 24235 is installed on the Z-direction guide 24234, the Z-direction linear module 24236 is installed on the Z-direction guide rail 24235 and is used for being connected with a liquid guide head end connector 24237 by a liquid guide device 24237, and a liquid guide connector 3792 is installed on the bottom of the Z-direction guide seat 24237. In actual operation, needle adapter 242310 is used for docking and unloading the needle, which needs to be replaced before absorbing different types of staining solution each time, and by arranging buffer spring 24239, the protection of the docking position of needle adapter 242310 and the tip of pipettor 24238 can be enhanced.

In this embodiment, the X-direction linear module 23, the Y-direction linear module 24233 and the Z-direction linear module 24236 have the same structure and each include a screw rod 232 distributed along the guide rail direction, a screw rod pair 233 is mounted on the screw rod 232, a slider 234 is mounted on the screw rod pair 233, and a servo motor 231 is mounted on one side of the screw rod 232 and connected with the screw rod 232. In actual operation, the screw 232 is precisely driven to rotate by the servo motor 231, and the slide block 234 is precisely moved by the cooperation between the screw 232 and the screw pair 233. In this embodiment, the top of the housing 241 of the mobile sample loading device 24 is fixed to the bottom of the slider 234 in the X-direction linear module 23, the Y-direction moving seat 24232 is fixed to the slider 234 of the Y-direction linear module 24233, and the Z-direction moving seat 24235 is fixed to the slider 234 of the Z-direction linear module 24236.

The automatic sampling mechanism of the slide dyeing machine is reasonable in design and simple in structure, and can realize accurate movement of a plurality of pipettes in X direction, Y direction and Z direction. In actual operation, the whole movable sample loading device 24 can be driven to precisely move in the X direction by the X-direction linear module 23, after the movable sample loading device 24 moves in the X direction, the Y-direction linear module 24233 arranged on each sample loading mechanism 2423 can control the independent movement of the plurality of sample loading mechanisms 2423 arranged on the Y-direction guide rail 2422 in the Y direction, and each sample loading mechanism 2423 can independently absorb the same or different staining solutions, so that a plurality of staining solutions can be absorbed at one time on the premise of not changing needles, and the sample loading efficiency is greatly improved by moving the sample loading device to the glass slide for staining at one time. After the sampling mechanism 2423 moves above the corresponding staining solution in the Y direction, the Z-direction linear module 24236 drives the pipette 24238 to move up and down along Z, so as to control the pipette 24238 to aspirate and reset the staining solution. After the plurality of pipettes 24238 absorb the corresponding staining solution, the X-direction linear module 23 drives the movable sample adding device 24 to integrally move to the glass slide for sample adding, so that the sample adding efficiency can be greatly improved.

The reagent classification storage mechanism 3 is arranged on the rack 1 and positioned below the automatic sampling mechanism 2, the reagent classification storage mechanism 3 comprises a storage rack 31, a storage cabinet 33 is arranged on the storage rack 31, a refrigeration storage box 34 and a normal-temperature storage box are arranged in the storage cabinet 33, the refrigeration storage box 34 comprises a refrigeration box body 341, the refrigeration box body 341 comprises a box body 3411, a refrigerator 3413 is arranged at the bottom of the box body 3411, an outer cover 3414 is arranged on the outer side of the refrigerator 3413, a plurality of sliding grooves 3412 are arranged in the box body 3411 at intervals in parallel, and the box body 3411 is always kept at a lower temperature through the refrigerator 3413 during actual operation so as to ensure the refrigeration environment of reagents. The front side of the refrigerator body 341 is provided with a sliding door 32 to facilitate the overall drawing and holding of the subsequent slide type reagent rack 342.

Install a plurality of parallel slide groove type reagent racks 342 that set up in the fridge body 341, slide groove type reagent racks 342 includes reagent rack body 3421, be provided with a plurality of parallel interval distribution's reagent box standing groove 3422 on the reagent rack body 3421, be used for loading the reagent box 343 embedding of refrigerated reagent in the reagent box standing groove 3422, the bottom of reagent rack body 3421 is provided with the protruding 3424 of slider of outwards protrusion, the protruding 3424 of slider is embedded in the spout 3412 that sets up on fridge body 341, through the structural design to slide groove type reagent rack 342, slide groove type reagent rack 342 passes through the cooperation of slider protrusion 3424 and spout 3412, wholly can follow the quick overall change of a plurality of reagent boxes 343 in the fridge 34, can make things convenient for the quick overall change of slide groove type reagent rack 342 to reach the purpose that strengthens reagent box 343 and change efficiency. The front end of the reagent rack body 3421 is provided with a handle 3423 which facilitates the drawing of the reagent rack body 3421, so as to facilitate the drawing operation of the reagent rack body 3421.

Two parallel open-type reagent storage tanks 35, two parallel partition-type reagent storage tanks 36 and four parallel closed-type reagent storage tanks 37 are arranged in the normal-temperature storage tank, and a needle insertion port 371 is formed in the top of each closed-type reagent storage tank 37. In actual operation, each reagent storage tank can be loaded with a corresponding staining reagent according to the requirements and marked.

An exhaust needle head collecting groove 38 is arranged on the outer side of the normal temperature storage box, and a needle head unhooking 39 is arranged in the exhaust needle head collecting groove 38. In the actual dyeing process, the needle of the liquid-transferring gun can absorb the corresponding dyeing reagent from the reagent box 343 in the cold storage box 34 or the reagent storage tank of the normal temperature storage box according to the requirement, gradually add the reagent into the glass slide, dye the tissue on the glass slide, when absorbing different dyeing reagents, the liquid-transferring gun needs to move to the waste gas needle collecting tank 38, remove the needle through the needle unhook 39 installed in the waste gas needle collecting tank 38, and then move to the needle replacement place to replace the needle, so as to avoid cross contamination between different dyeing reagents.

The reagent classification storage mechanism 3 of the glass slide dyeing machine has reasonable design, simple structure and convenient operation, can classify and store different types of staining solutions, and is convenient for the automatic suction and replacement of the pipette tips. In actual operation, only the staining solution to be refrigerated is placed on the sliding groove type reagent rack 342 of the refrigerated storage box 34 through the reagent kit 343, and through the structural design of the sliding groove type reagent rack 342, the sliding groove type reagent rack 342 can be rapidly pulled out or inserted from the refrigerated storage box 34 through the matching of the sliding block bulge 3424 and the sliding groove 3412, and the rapid overall replacement of a plurality of reagent kits 343 on the sliding groove type reagent rack 342 can be facilitated, so that the aim of enhancing the replacement efficiency of the reagent kit 343 is fulfilled. The staining solution which can be stored at normal temperature may be added to the open reagent storage tank 35, the partitioned reagent storage tank 36, and the closed reagent storage tank 37 of the normal temperature storage tank for classification.

Install slide glass shake mechanism 4 in reagent classification storage mechanism 3 one side, slide glass shake mechanism 4 includes bottom plate 41, install water bath mechanism 42 on the bottom plate 41, water bath mechanism 42 includes water bath 421, water bath 421 fixed mounting is on bottom plate 41, install heater 422 in the water bath 421, be provided with a plurality of inductors on the top end edge of water bath 421 and reserve mounting hole 423, install the inductor in the inductor reservation mounting hole 423. During actual operation, the temperature in the water bath 421 can be monitored in real time through the temperature sensor arranged in the sensor reserved mounting hole 423, and the water temperature in the water bath 421 can be controlled through the heater 422, and the top end edge of the water bath 421 is provided with a positioning mounting groove 424 for being matched with the positioning embedding of the protective housing 43, so that rapid and accurate alignment and mounting between the water bath 421 and the protective housing 43 are realized.

The protection shell 43 is installed on the outer side of the water bath mechanism 42, the slide loading shaking mechanism 44 capable of overturning and shaking is installed above the water bath mechanism 42 on the protection shell 43, the slide loading shaking mechanism 44 comprises a rotating shaft 444, the rotating shaft 444 is installed on the protection shell 43 in a penetrating mode transversely, a shaking servo motor 45 is installed on the protection shell 43 and is connected with one end of the rotating shaft 444 through a coupler, bearings 445 are installed at the butt joint positions of the rotating shaft 444 and the protection shell 43 to ensure smooth rotation of the rotating shaft 444, a loading frame 441 is installed on the rotating shaft 444, the loading frame 441 is located above the water bath mechanism 42, the loading frame 441 is divided into a plurality of channels for loading slide vertical supports through a plurality of partition strips 442 arranged at intervals in parallel, positioning partition plates 443 are correspondingly arranged on the rear side of each channel, the channels are used for loading the slide vertical supports 100, and the positioning partition plates 443 are used for positioning the slide vertical supports 100.

The slide shaking mechanism 4 is reasonable in design, simple in structure and convenient to operate, the placing quantity of the slides can be greatly increased, and the slides after the staining solution is dripped are subjected to inclined shaking, so that the staining effect is improved. In actual operation, the vertical slide rack 100 is only required to be placed on the vertical slide rack placing channel provided on the loading rack 441, and is positioned and fixed by the positioning partition 443, and then the vertical stained slide 200 is placed on the vertical slide rack 100, so that the placement number of the stained slide 200 can be greatly increased by the structural design. After the slide glass dyeing machine works, a plurality of dyeing liquids are sequentially dripped into the vertically placed dyeing slide glass 200, the water bath mechanism 42 is filled with the hot water, the shaking servo motor 45 drives the rotating shaft 444 to drive the loading frame 441 to turn upwards for a certain angle at intervals, the dyeing slide glass 200 is taken out of the water bath tank 421 in the water bath mechanism 42, and then the shaking servo motor 45 periodically drives the rotating shaft 444 to drive the loading frame 441 to rotate back and forth in a certain angle at a certain frequency, so that shaking of the dyeing slide glass 200 placed on the loading frame 441 is realized, mixing among different dyeing liquids is accelerated, and therefore, the dyeing effect can be enhanced, and the dyeing efficiency is greatly improved. After shaking for a period of time, the shaking servo motor 45 drives the loading frame 441 to reset, and the dyed glass slide 200 reenters the water bath tank 421 of the water bath mechanism 42 to be heated in a water bath.

In this embodiment, a plurality of lockers 11 are provided with in the lower half of frame 1 to make things convenient for the storing, be located automatic sampling mechanism 2, reagent classification storage mechanism 3 and slide glass shake the outside of mechanism 4 and install dustcoat 12 in frame 1, the front side of dustcoat 12 is provided with qianmen 13, installs transparent observation window 14 on qianmen 13, in order to make things convenient for the protection and the observation to automatic sampling mechanism 2, reagent classification storage mechanism 3 and slide glass shake the mechanism 4, a plurality of removal wheels 15 are installed to the bottom of frame 1, in order to make things convenient for the bulk movement of equipment.

This full-automatic intelligent slide glass dyeing equipment is automatic, intelligent degree is high, through the structural design to dyeing equipment, can increase the quantity of placing of slide glass by a wide margin to slide glass after the dropwise add staining solution is inclined shakes, improves the dyeing effect, and can carry out classified storage to different types of staining solution, improves application of sample efficiency.

The foregoing is a preferred embodiment of the present invention, but the present invention should not be limited to the embodiment and the disclosure of the drawings, so that the equivalents and modifications can be made without departing from the spirit of the disclosure.

Claims (10)

1. Full-automatic intelligent slide glass dyeing equipment, its characterized in that includes

A frame;

an automatic sampling mechanism mounted on the frame;

the reagent classifying and storing mechanism is arranged on the rack and positioned below the automatic sampling mechanism and comprises a storing rack, a storing cabinet is arranged on the storing rack, a refrigerating storage box and a normal temperature storage box are arranged in the storing cabinet, the refrigerating storage box comprises a refrigerating box body, a plurality of sliding chute type reagent racks which are arranged in parallel are arranged in the refrigerating box body, the sliding chute type reagent racks comprise a reagent rack body, a plurality of reagent box placing grooves which are distributed at intervals in parallel are arranged on the reagent rack body, a reagent box for loading refrigerating reagent is embedded in the reagent box placing grooves, a sliding block protrusion protruding outwards is arranged at the bottom of the reagent rack body and is embedded in a sliding chute arranged on the refrigerating box body, a sliding door which is convenient for taking the reagent rack body is arranged at the front side of the refrigerating box body, and a plurality of types of reagent storing grooves are arranged in the normal temperature storage box;

install slide glass shake mechanism in reagent classified storage mechanism one side, slide glass shake mechanism including installing the bottom plate in the frame, install water bath mechanism on the bottom plate, the shell is installed in the water bath mechanism outside, but the slide glass that lies in water bath mechanism on the shell is loaded and shake mechanism that shakes of overturning is installed to the top that lies in water bath mechanism, slide glass is loaded and shake mechanism includes the pivot, the pivot is along transversely running through installing on the shell, pivot and shell butt joint department all install the bearing, shake servo motor and install on the shell and be connected with the one end of pivot through the shaft coupling, install the loading frame in the pivot, the loading frame is located the top of water bath mechanism, the loading frame separates into a plurality of channels that are used for loading slide glass to erect the support through the partition strip that many parallel intervals set up, and the rear side of every passageway all corresponds and is provided with the location baffle.

2. A fully automatic intelligent slide staining apparatus according to claim 1 wherein: the water bath mechanism comprises a water bath tank, the water bath tank is fixedly arranged on a bottom plate, a heater is arranged in the water bath tank, a plurality of inductor reserved mounting holes are formed in the top end edge of the water bath tank, an inductor is arranged in each inductor reserved mounting hole, and a positioning mounting groove matched with the positioning embedding of the shell is formed in the top end edge of the water bath tank.

3. A fully automatic intelligent slide staining apparatus according to claim 1 wherein: the automatic sample adding mechanism comprises a sample adding frame arranged on the frame, an X-direction guide rail is arranged at the top of the sample adding frame along the X-direction, an X-direction linear module is arranged on the X-direction guide rail, a movable sample adding device is arranged at the bottom of the X-direction linear module and comprises a shell, the top of the shell is fixed at the bottom of the X-direction linear module, a sample adding assembly is arranged in the shell, the sample adding assembly comprises a mounting frame fixed at the inner side of the shell, Y-direction guide rails are arranged on the mounting frame along the Y-direction, a plurality of sample adding mechanisms which are independently controlled are arranged on the Y-direction guide rails, the plurality of sample adding mechanisms are identical in structure and comprise Z-direction brackets, the Z-direction brackets are arranged on the Y-direction guide rails through Y-direction movable seats, Y-direction linear modules for driving the Z-direction brackets to move along the Y-direction guide rails are arranged on the Y-direction linear modules, the Z-direction movable seats are arranged on the Z-direction guide rails, the Z-direction linear brackets are used for being arranged on the Z-direction linear modules, the Z-direction movable seats are arranged on the Z-direction guide rails, the Z-direction linear brackets are used for being arranged on the Z-direction guide bases, the Z-direction linear seat is arranged on the vertical displacement seat is arranged on the Z-direction guide seat, and the Z-direction guide seat is arranged on the vertical displacement seat.

4. An automated loading mechanism for a slide staining machine according to claim 3 wherein: the end of the liquid transfer device is provided with a needle head joint, and the butt joint part of the needle head joint and the end of the liquid transfer device is provided with a buffer spring.

5. An automated loading mechanism for a slide staining machine according to claim 3 wherein: the X-direction linear module, the Y-direction linear module and the Z-direction linear module are identical in structure and comprise screw rods distributed along the direction of the guide rail, screw rod pairs are arranged on the screw rods, sliding blocks are arranged on the screw rod pairs, and a servo motor is arranged on one side of the screw rods and connected with the screw rods.

6. An automated loading mechanism for a slide staining machine according to claim 5 wherein: the top of the shell of the movable sample adding device is fixed at the bottom of the sliding block in the X-direction linear module, the Y-direction movable seat is fixed on the sliding block of the Y-direction linear module, and the Z-direction movable seat is fixed on the sliding block of the Z-direction linear module.

7. A fully automatic intelligent slide staining apparatus according to claim 1 wherein: the refrigerator body comprises a box body, a refrigerator is arranged at the bottom of the box body, an outer cover is arranged on the outer side of the refrigerator, a plurality of sliding grooves which are arranged at intervals in parallel are formed in the box body, and sliding blocks arranged at the bottom of the reagent frame body are embedded in the sliding grooves.

8. A fully automatic intelligent slide staining apparatus according to claim 1 wherein: the normal temperature storage box is internally provided with two parallel open-type reagent storage tanks, two parallel partition-type reagent storage tanks and four parallel closed-type reagent storage tanks, and the top of each closed-type reagent storage tank is provided with a needle insertion port.

9. A fully automatic intelligent slide staining apparatus according to claim 1 wherein: the waste gas needle head collecting groove is arranged on the outer side of the normal temperature storage box, and a needle head unhooking device is arranged in the waste gas needle head collecting groove.

10. A fully automatic intelligent slide staining apparatus according to claim 1 wherein: the automatic sampling machine is characterized in that an outer cover is arranged on the outer side of the rack, which is positioned on the automatic sampling mechanism, the reagent classification storage mechanism and the glass slide shaking mechanism, a front door is arranged on the front side of the outer cover, a transparent observation window is arranged on the front door, and a plurality of moving wheels are arranged at the bottom of the rack.