A kind of Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages
Technical field
The utility model is related to chemiluminescence immune assay field more particularly to a kind of possess multiple independent reaction passages
Full-automatic chemiluminescence immunoassay analysis meter.
Background technology
The advantages such as chemiluminescence immune assay has high sensitivity, high specificity, the range of linearity is wide, the degree of automation is high are special
Point, it has also become the main means of clinical diagnosis.But domestic automatic chemiluminescence immunoassay is started late, clinical practice
Have developed rapidly, originally the only detection projects such as sex hormone, thyroid hormone are detected with chemoluminescence method, with technology into
One step develops, and tumor markers, the disease marker for being metabolized class substance, infectious disease marker, treating cardiac and cerebral vascular diseases etc. are detectable
Project is more and more, meanwhile, chemiluminescence immunoassay detection also has its uncertainty, which is mainly derived from test operation
And detection architecture.
The general indispensable module of Full-automatic chemiluminescence immunoassay analysis meter have sample-adding module, plus magnetic bead module, raising module,
Cleaning module, reagent adding module, assay readings module composition.Existing chemical illumination immunity analysis instrument is usually by these functions
Module conspires to create the assembly line of single thread, and operational process is generally as shown in Figure 1.
But due to single the pipeline design, when Full-automatic chemiluminescence immunoassay analysis meter has pressed some operation beat
Design is difficult to again while compatible other operation beats, instrument can only just run some and be incubated duration or reaction step simultaneously
Reagent.Therefore, there are following defects for existing Full-automatic chemiluminescence immunoassay analysis meter:1st, do not support to run different incubations simultaneously
The reagent of duration;2nd, do not support to run one-step method, two-step method reagent 3 simultaneously, due to single the pipeline design, test needs one
An a queuing takes turns doing;Even if the 4, loading emergency treatment detection, also need to come after the test of operation.
Utility model content
For the utility model to solve the above problem of the prior art, proposition is a kind of to possess the complete of multiple independent reaction passages
Robotics luminescence immunoassay instrument.
The Full-automatic chemiluminescence immunoassay analysis meter provided by the utility model for possessing multiple independent reaction passages is supported same
The different reagents for being incubated duration of Shi Yunhang are supported to run one-step method, two-step method reagent simultaneously, can load emergency treatment detection at any time.
To achieve the above object, the utility model uses following technical scheme:
The first aspect of the utility model is to provide a kind of Full-automatic chemiluminescence for possessing multiple independent reaction passages
Immunity analysis instrument, including several reaction channels being arranged side by side between sample-adding module and assay readings module;Wherein, it is described anti-
Passage is answered to be made of horizontal displacement motor, conveyer belt, incubation storehouse, reagent strip and extraction rifle, the incubation storehouse is installed on described defeated
It send and takes, the reagent strip is placed in the incubation storehouse, and Y-direction shifting is done by the horizontal displacement motor driving incubation storehouse
Dynamic, the extraction rifle is arranged above the conveyer belt and can do Z-direction movement.
Further, in the Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages, institute
Stating has several apertures on reagent strip, bar magnet set is placed with built in one of them described aperture.
It is further preferred that in the Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages
In, the extraction rifle includes the extraction rifle pipette tips that can be connected with bar magnet set interference, positioned at the extraction rifle pipette tips axial line
On for adsorbing the permanent magnet of magnetic bead, the permanent magnet is driven by extraction motor and relatively described rifle pipette tips of extracting move up and down.
Further more preferably, in the automatic chemiluminescence immunoassay for possessing multiple independent reaction passages
In instrument, the extraction motor is installed on the extraction rifle top, and the extraction motor is connected by motor shaft and the permanent magnet
It connects.
It is further preferred that in the Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages
In, inner wall or its outer wall interference that the extraction rifle pipette tips can be covered with the bar magnet are connected.
Further preferably preferably, in the automatic chemiluminescence immunoassay for possessing multiple independent reaction passages
In instrument, the extraction rifle is moved up and down by the length travel motor driving being arranged on substrate.
Further more preferably, it is immunized point in the Full-automatic chemiluminescence for possessing multiple independent reaction passages
In analyzer, the output shaft of the length travel motor is equipped with screw rod, and the screw rod is equipped with nut, and the nut is carried with described
It takes arms and is fixedly connected.
It is further preferred that in the Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages
In, the assay readings module is arranged at the end of the conveyer belt, and can be X to movement, for each reaction channel
Reagent strip assay readings after the completion of upper reaction.
It is further preferred that in the Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages
In, the incubation storehouse is equipped with heating module, for being heated to reaction required temperature to the reagent strip.
It is further preferred that in the Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages
In, the reaction channel is 6-18, and is arranged side by side.
The utility model uses above-mentioned technical proposal, compared with prior art, has the following technical effect that:
The Full-automatic chemiluminescence immunoassay analysis meter provided by the utility model for possessing multiple independent reaction passages, by setting
In respect of multiple independent reaction channels, each reaction channel is made to have independent incubation and magnetic bead forwarding function, it can be achieved that multi-thread
Journey runs different tests simultaneously;The magnetic bead being made of extraction rifle and bar magnet set shifts binding structure, can stablize, reliably by magnetic bead
It is transferred in sample, washing lotion or reagent, and is designed without fluid path, reduce rate of breakdown, ensure that chemical illumination immunity analysis instrument
Overall performance;After tested, the Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages has great
Flexibility can support the reagent of different incubation durations to run simultaneously, one-step method, the reagent of two-step method is supported to run simultaneously, is supported
Emergency treatment detection is loaded at any time, improves the scope of application of instrument.
Description of the drawings
Fig. 1 is the operational process schematic diagram of existing Full-automatic chemiluminescence immunoassay analysis meter;
Fig. 2 is the operational process schematic diagram of the utility model Full-automatic chemiluminescence immunoassay analysis meter;
Fig. 3 is the overall structure diagram of the utility model Full-automatic chemiluminescence immunoassay analysis meter;
Fig. 4 is the structure diagram of single reaction channel in the utility model Full-automatic chemiluminescence immunoassay analysis meter;
Fig. 5 is the cross section structure schematic diagram that rifle is extracted in the utility model Full-automatic chemiluminescence immunoassay analysis meter;
Fig. 6 is that rifle permanent magnet and magnetic when drawing magnetic bead are extracted in the utility model Full-automatic chemiluminescence immunoassay analysis meter
The location state diagram of stick set;
Fig. 7 is that rifle permanent magnet and magnetic when eluting magnetic bead are extracted in the utility model Full-automatic chemiluminescence immunoassay analysis meter
The location state diagram of stick set;
Fig. 8 is that the utility model possesses the Full-automatic chemiluminescence immunoassay analysis meter of multiple independent reaction passages applied to two
The structure diagram of footwork reagent strip;
Wherein, each reference numeral is:
1- horizontal displacement motors, 2- conveyer belts, 3- are incubated storehouse, 4- reagent strips, 5- bar magnet sets, 6- length travel motors, 7-
Substrate, 8- screw rods, 9- nuts, 10- extraction motors, 11- extraction rifles, 12- extraction rifle pipette tips, 13- assay readings modules, 14- electricity
Arbor, 15- permanent magnets, 16- magnetic beads.
Specific embodiment
The utility model is described in more detail below by specific embodiment, for a better understanding of this reality
With new, but following embodiments are not intended to limit the scope of the utility model.
As Figure 2-3, the present embodiment provides a kind of full-automatic chemical hairs for possessing multiple independent reaction passages for embodiment 1
Light immunity analysis instrument, including several reaction channels being arranged side by side between sample-adding module and assay readings module 13;Wherein, instead
Passage is answered to be made of horizontal displacement motor 1, conveyer belt 2, incubation storehouse 3, reagent strip 4 and extraction rifle 11, storehouse 3 is incubated and is installed on conveying
On band 2, reagent strip 4, which is placed in, to be incubated in storehouse 3, by horizontal displacement motor 1 incubation storehouse 3 is driven to do Y-direction movement, extraction rifle 11 is set
Above conveyer belt 2 and Z-direction movement can be done.
In the Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages in the present embodiment, such as Fig. 3-4 institutes
Show that there are several apertures on reagent strip 4, bar magnet set 5 is placed with built in one of aperture, this is used for the aperture for putting bar magnet set 5
It is first aperture of the front end on reagent strip 4.
As shown in figure 5, extraction rifle 11 includes the extraction rifle pipette tips 12 that can be connected with bar magnet 5 interference of set, positioned at extraction rifle rifle
For adsorbing the permanent magnet 15 of magnetic bead 16 on first 12 axial line, permanent magnet 15 is driven by extraction motor 10 and opposite extraction rifle pipette tips
12 move up and down, and extract bar magnet set 5 by extracting rifle pipette tips 12, then cover 5 by the relative displacement bar magnet of permanent magnet 15
Carrying magnetic loses magnetism, and is then shifted by 5 absorption magnetic bead 16 of bar magnet set.
As shown in figure 5, extraction motor 10 is installed on 11 top of extraction rifle, and extracts motor 10 and pass through motor shaft 14 and permanent magnetism
Iron 15 connects.Permanent magnet 15 is installed in the lower end of motor shaft 14, and moving up and down for motor shaft 14 is controlled by extracting motor 10, after
And the permanent magnet 15 of its lower end is driven to move up and down, the bottom away from or close to bar magnet set 5 so that the bottom belt magnetic of bar magnet set 5
Property loses magnetism, for the transfer of magnetic bead 16.
Possess the Full-automatic chemiluminescence immunoassay analysis meter of multiple independent reaction passages as the present embodiment one is preferred
Technical solution, extraction rifle pipette tips 12 can cover 5 inner wall with bar magnet or its outer wall interference be connected, specifically, extract rifle pipette tips 12 with
Extraction rifle 11 is whole to be moved down, and extraction rifle pipette tips 12 are cylindrical in shape, and the internal diameter that can design extraction rifle pipette tips 12 is slightly less than bar magnet set 5
Outer diameter, make 12 interference of extraction rifle pipette tips pack tightly bar magnet set 5 outer wall on, during packing tightly, due to by extraction rifle pipette tips
Certain deformation occurs for 12 downward power effects, bar magnet set 5;Or the outer diameter of design extraction rifle pipette tips 12 is slightly larger than bar magnet set 5
Internal diameter makes 12 interference of extraction rifle pipette tips be close on the inner wall of bar magnet set 5, during fitting, due to by extraction rifle pipette tips
Certain deformation occurs in extrusion process for 12 downward power effects, bar magnet set 5.
Please continue to refer to such as Fig. 4, extraction rifle 11 by the driving of length travel motor 6 being arranged on substrate 7 move down
Dynamic, substrate 7 is fixed on 2 top position of conveyer belt, and length travel motor 6 is fixedly mounted on substrate 7, and is connected with extraction rifle 11,
For driving extraction rifle 11 is whole to move up and down.As a preferred technical solution, the output shaft of length travel motor 6
Screw rod 8 is equipped with, screw rod 8 is equipped with nut 9, and nut 9 is fixedly connected with extraction rifle 11, i.e., length travel motor 6 passes through screw rod 8
The extraction rifle 11 on screw rod 8 is driven to move up and down.
As shown in Fig. 2, assay readings module 13 is arranged at the end of conveyer belt 2, and can be X to movement, for each anti-
Answer 4 assay readings of reagent strip after the completion of being reacted on passage.It is incubated storehouse 3 and is equipped with heating module, for being heated to reagent strip 4
React required temperature.In addition, on the Full-automatic chemiluminescence immunoassay analysis meter for possessing multiple independent reaction passages, reaction is logical
Road is 6-18, is preferably 12, and is arranged side by side, each reaction channel is by horizontal displacement motor 1, conveyer belt 2, incubation
Storehouse 3, reagent strip 4 and the extraction composition composition of rifle 11, have independent Y, Z driving, do not restrict between each other.
The extraction rifle that embodiment 2 possesses in the Full-automatic chemiluminescence immunoassay analysis meter of multiple independent reaction passages carries out magnetic
The method of pearl transfer, specifically comprises the following steps:
Step 1:Take bar magnet set
Bar magnet set 5 is moved to the underface of extraction rifle 11, moves downward extraction rifle 11, extraction rifle pipette tips 12 probe into magnetic
In stick set 5;Under the extruding of extraction rifle 11, bar magnet set 5 links together with extraction 12 interference of rifle pipette tips;Make again extraction rifle 11 to
Upper movement, while extract rifle pipette tips 12 and moved up with bar magnet set 5, take out bar magnet set 5;
Step 2:Draw magnetic bead
After having taken bar magnet set 5, the aperture that will draw magnetic bead on reagent strip 4 is moved to the underface of extraction rifle 11,
By extraction motor 10 permanent magnet 15 is driven to be moved down into the bottom of bar magnet set 5, the bottom belt of bar magnet set 5 is made to be magnetic;Then
Extraction rifle 11 is moved down, the bottom of bar magnet set 5 is insinuated into the liquid containing magnetic bead 16, then extraction rifle is made to delay about 11
Slow mobile, magnetic bead can be drawn onto the bottom of bar magnet set 5 by permanent magnet 15;Finally, move up extraction rifle 11, while extract rifle rifle
First 12 move up with bar magnet set 5, and magnetic bead 16 is also moved to bar magnet set 5 outside liquid;When drawing magnetic bead, permanent magnet 15 and magnetic
The location status of stick set 5 is as shown in Figure 6:
Step 4:Elute magnetic bead
After drawing magnetic bead 16, the aperture that will receive magnetic bead 16 on reagent strip 4 is moved to the underface of extraction rifle 11,
Extraction rifle 11 is moved down, the bar magnet for being adsorbed with magnetic bead 16 set 5 is insinuated into the liquid of magnetic bead 16 to be received, by extraction electricity
Machine 10 drives permanent magnet 15 to move up, and the bottom of bar magnet set 5 is made to lose magnetism;Then make extraction rifle very fast by a small margin about 11
Speed movement, magnetic bead 16 is eluted in liquid;When eluting magnetic bead, the location status of permanent magnet 15 and bar magnet set 5 is as shown in Figure 7:
Step 4:Magnetic bead mixing
Elute magnetic bead 16 after, by reagent strip 4 will mixing magnetic bead 16 aperture run to extraction rifle 11 underface,
This will mixing magnetic bead 16 aperture refer to be incubated during aperture, bar magnet cover 5 bottoms it is nonmagnetic in the state of, make extraction
Rifle 11 moves downward, by the bottom of bar magnet set 5 be insinuated into will in the liquid of mixing magnetic bead 16, make extraction rifle about 11 by a small margin
It faster moves, 16 mixing of magnetic bead in liquid is kept into suspended state;
Step 5:Bar magnet set is beaten de-
When bar magnet is no longer needed to cover 5, the bar magnet set putting hole on reagent strip 4 is moved to the underface of extraction rifle 11,
By extraction motor 10 permanent magnet 15 is driven to move downward, until bar magnet set 5 is de- from top in extraction rifle pipette tips 12 so that bar magnet set
5 drop in the bar magnet set putting hole on reagent strip 4.
In the magnetic bead transfer method of the present embodiment updraft type magnetic bead transfer binding structure, during step 4 magnetic bead mixing, permanent magnet
15 and bar magnet set 5 location status for permanent magnet 15 lower end away from bar magnet set 5 bottom, make bar magnet cover 5 bottoms lose magnetism,
Permanent magnet 15 is identical with the location status of bar magnet set 5 when eluting magnetic bead with step 3.
Embodiment 3 is using the Full-automatic chemiluminescence immunoassay analysis meter operation two-step method examination for possessing multiple independent reaction passages
Agent, wherein, take bar magnet set, draw magnetic bead, magnetic bead elution, magnetic bead mixing, bar magnet set are beaten to take off etc. and be acted referring to above-described embodiment 2,
Shown in two-step method reagent strip Structure Figure 8, carrying out practically includes the following steps:
The first step:Incubation storehouse 3 is heated to the reaction temperature needed for reagent;
Second step:Reagent strip 4 is put into and is incubated in storehouse 3;
3rd step:Sample-adding module manual or using equipment matching pipettes sample to No. 4 holes position from sample tube;
4th step:Magnetic bead extraction rifle 11 takes the bar magnet on No. 0 position on reagent strip 4 to cover 5;
5th step:Magnetic bead 16 from No. 3 holes position on reagent strip 4 is drawn and come out by magnetic bead extraction rifle 11;
6th step:In No. 4 holes that magnetic bead 16 is eluted in the reagent strip 4 of existing sample by magnetic bead extraction rifle 11;
7th step:Incubation reaction X minutes in magnetic bead and No. 4 holes of the sample on reagent strip 4.It can be mixed therebetween with magnetic bead
It is even;
8th step:Magnetic bead from No. 4 holes position on reagent strip 4 is drawn and come out by magnetic bead extraction rifle 11;
9th step:Magnetic bead 16 is eluted to No. 5 holes on the reagent strip 4 containing 16 cleaning solution of magnetic bead by magnetic bead extraction rifle 11
In;
Tenth step:Magnetic bead from No. 5 holes position on reagent strip 4 is drawn and come out by magnetic bead extraction rifle 11;
11st step:Magnetic bead is eluted in No. 6 holes on the reagent strip 4 containing magnetic bead cleaning solution by magnetic bead extraction rifle 11;
12nd step:Magnetic bead from No. 6 holes position on reagent strip 4 is drawn and come out by magnetic bead extraction rifle 11;
13rd step:Magnetic bead is eluted in No. 7 holes on the reagent strip 4 containing mark working liquid by magnetic bead extraction rifle 11;
14th step:Incubation reaction X minutes in magnetic bead and No. 7 holes of the mark working liquid on reagent strip 4;It therebetween can be adjoint
Magnetic bead mixing;
15th step:Magnetic bead from No. 7 holes position on reagent strip 4 is drawn and come out by magnetic bead extraction rifle 11;
16th step:Magnetic bead is eluted in No. 8 holes on the reagent strip 4 containing magnetic bead cleaning solution by magnetic bead extraction rifle 11;
17th step:Magnetic bead from No. 8 holes position on reagent strip 4 is drawn and come out by magnetic bead extraction rifle 11;
18th step:Magnetic bead is eluted in No. 9 holes on the reagent strip 4 containing magnetic bead cleaning solution by magnetic bead extraction rifle 11;
19th step:Magnetic bead from No. 9 holes position on reagent strip 4 is drawn and come out by magnetic bead extraction rifle 11;
20th step:Magnetic bead is eluted in No. 10 holes on the reagent strip 4 containing preexciting liquid by magnetic bead extraction rifle 11;
21st step:It is incubated storehouse 3 to run in assay readings module 13 with reagent strip 4, assay readings;
22nd step:Magnetic bead extracts rifle 11 and beats bar magnet set in No. 0 hole taken off on reagent strip 4;
23rd step:It is incubated storehouse 3 and runs to initial position with reagent strip 4, take reagent strip 4 away, experiment terminates.
The Full-automatic chemiluminescence immunoassay analysis meter provided by the utility model for possessing multiple independent reaction passages, it can be achieved that
Multithreading runs different tests simultaneously;After tested, which is immunized point
Analyzer has great flexibility, and the reagent of different incubation durations can be supported to run simultaneously, support the examination of one-step method, two-step method
Agent is run simultaneously, is supported to load emergency treatment detection at any time, is improved the scope of application of instrument.
Specific embodiment of the utility model is described in detail above, but it is intended only as example, and this practicality is new
Type is not restricted to particular embodiments described above.To those skilled in the art, it is any that the utility model is carried out
Equivalent modifications and substitute also all among the scope of the utility model.Therefore, the spirit and model of the utility model are not being departed from
Lower made impartial conversion and modification are enclosed, should all be covered in the scope of the utility model.