JP2010032215A - Autoanalyzer - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an autoanalyzer capable of simplifying the operation of B/F separation. <P>SOLUTION: The autoanalyzer is equipped with cuvets, a sample dispensing means and a reagent dispensing means both of which respectively dispense a sample and a reagent to the cuvets, a reaction turntable holding a plurality of the cuvets circumferentially and subjecting the sample and the reagent to antigen-antibody reaction while rotating, a B/F separating table for separating the liberated antigen (or antibody) F contained in the cuvets after the antigen-antibody reaction from the antigen (or antibody) B bonded to a magnetic solid phase and injecting the detection reagent reacting with the antigen (or antibody) bonded to the magnetic solid phase after B/F separation and a detector for quantifying the reacting detection reagent. A first cuvet transfer means for transferring the cuvets to the B/F separation table from the reaction table is present between the reaction table and the B/F separation table. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a target antigen (or antibody) that did not bind to a solid phase antibody (or solid phase antigen) and a labeled antibody (or labeled antigen) that did not bind to the target antigen (or antibody) in an immunoassay. ) From the reaction vessel.

  An immunoassay is a measurement method for quantifying a specific antigen or antibody in a living body by utilizing a specific antigen-antibody reaction, which is similar to “key and keyhole”. Because of its extremely high detection sensitivity, it is widely used for the quantification of substances present in trace amounts in the living body, such as cancer markers, antigens of various infectious diseases, and hormones. Samples to be measured are body fluids such as blood (particularly serum), urine, saliva, spinal fluid. As immunoassay methods, chemiluminescence immunoassay (CLIA method), chemiluminescence enzyme immunoassay (CLEIA method), enzyme immunoassay (EIA method) and the like are used in the clinical laboratory field.

  FIG. 1 shows an example of the principle of the EIA method. FIG. 1A shows an initial state of the EIA method. In the figure, 1 is a bead solid phase. For example, beads made of a material such as polystyrene are used. The antibody (a kind of protein) is adsorbed on the surface of the bead solid phase 1 to form the solid phase antibody 2. When a sample containing the antigen 3 to be measured is added and reacted with the bead solid phase 1 for a certain period of time, the antigen 3 causes an antigen-antibody reaction with the solid phase antibody 2 and binds to the surface of the bead solid phase 1. Then, an antigen-antibody complex 4 is formed as shown in FIG. The antigen in this state is referred to as a bound antigen 5 (abbreviated as Bound form, B), and the binding is not cut by washing the bead solid phase 1. On the other hand, the antigen 3 unbound to the solid phase antibody 2 is called free form antigen (abbreviated as F), and can be easily removed from the reaction vessel by washing the bead solid phase 1. . The operation of separating the bound antigen 5 and the free antigen 3 by washing is called B / F separation. After the B / F separation, only the bound antigen 5 remains in the reaction vessel as shown in FIG.

  Next, in the EIA method, an antibody (enzyme-labeled antibody) 6 labeled with an appropriate enzyme is bound to the bound antigen 5 forming the antigen-antibody complex 4 by an antigen-antibody reaction, and the bound type An operation of labeling the antigen 5 with an enzyme is performed. FIG. 1 (d) shows this situation. When enzyme-labeled antibody 6 labeled with labeling enzyme 7 is added to the reaction vessel and reacted with bead solid phase 1 for a certain period of time, enzyme-labeled antibody 6 is sandwiched between bound antigen 5 and solid-phase antibody 2. To form an enzyme-labeled antibody-antigen-antibody complex 8. At this time, in the EIA method, in order to sufficiently label the bound antigen 5 bound to the solid phase antibody 2, an excess amount of the enzyme-labeled antibody 6 is typically added. Therefore, after labeling the bound antigen 5 by the antigen-antibody reaction between the bound antigen 5 and the enzyme-labeled antibody 6, the enzyme-labeled antibody 9 bound to the antigen and the extra free enzyme label not bound to the antigen. It is necessary to perform the operation of separating the antibody 6, that is, the B / F separation again. After this B / F separation, only the enzyme-labeled antibody-antigen-antibody complex 8 labeled with an enzyme remains on the surface of the bead solid phase 1 as shown in FIG. This means that as many labeled enzymes 7 as the number of bound antigens 5 remaining on the surface of the bead solid phase 1 remain.

The amount of the labeled enzyme 7 bound to the surface of the bead solid phase 1 is, as shown in FIG. 1 (f), added with a coloring reagent 10 that can be a substrate of the enzyme and reacted with the labeled enzyme 7 to generate the colored enzyme. By measuring the amount of the coloring reagent 10 ^′ , it can be quantified.

  In the above example, the method for quantifying an antigen using a solid phase antibody and an enzyme-labeled antibody has been described. However, the same operation is performed using a solid phase antigen and an enzyme-labeled antigen instead of the solid phase antibody and the enzyme-labeled antibody. Can be used to quantify the antibody in the sample.

  As described above, in the EIA method, B / F separation for separating an antigen (or antibody) bound to a bead solid phase and an unbound free antigen (or antibody) by an antigen-antibody reaction, and an antigen-antibody B / F separation for separating the labeled antibody (or labeled antigen) bound to the complex from the unbound free labeled antibody (or labeled antigen) is an important key for determining the accuracy of measurement.

  Usually, B / F separation is performed by pouring washing water into the reaction vessel containing the solid phase to wash away free antigen (or antibody) and labeled antibody (or labeled antigen). And the residual water after washing | cleaning is removed from a reaction container by sucking up with a suction nozzle.

JP 7-318559 A

  By the way, in a conventional automatic analyzer that performs such B / F separation, a reaction unit that reacts a sample and a reagent in a reaction vessel, and a B / F separation unit that removes unbound substances of a detection target substance or reagent. Is arranged on a single line or turntable.

  For this reason, when a B / F separation unit is provided on a single turntable, the timing of B / F separation varies depending on the inspection item, and therefore, a plurality of nozzles, magnets, and agitation mechanisms move separately during cleaning. Necessity comes out.

  In addition, there is a trade-off between general biochemical analysis that does not include the B / F separation step and immunological analysis that includes the B / F separation step, and the operation of a plurality of nozzles, magnets, and stirring mechanisms becomes extremely complicated. There was a problem that.

  An object of the present invention is to provide an automatic analyzer capable of simplifying the operations of a plurality of nozzles, magnets, and agitation mechanisms used for B / F separation in view of the above points.

In order to achieve this object, an automatic analyzer according to the present invention provides:
(1) a cuvette for reacting a sample with a reagent;
(2) a sample dispensing means and a reagent dispensing means for respectively dispensing a sample and a reagent into the cuvette;
(3) a plurality of the cuvettes in the circumferential direction, and a reaction turntable that causes the sample and the reagent to undergo an antigen-antibody reaction while rotating;
(4) The released antigen (or antibody) F contained in the cuvette after the antigen-antibody reaction is separated from the antigen (or antibody) B bound to the magnetic solid phase, and after the B / F separation, the magnetic solid A B / F separation table for injecting and reacting a detection reagent that reacts with an antigen (or antibody) bound to a phase;
(5) a detector for quantifying the reacted detection reagent,
Between the reaction turntable and the B / F separation table, there is a first cuvette transfer means for transferring the cuvette from the reaction turntable to the B / F separation table.

The B / F separation table is
(1) collecting the magnetic solid phase on the cuvette wall with a magnet;
A step of sucking and separating the waste liquid from the assembled magnetic solid phase;
Injecting cleaning water;
A plurality of B / F separation positions for performing a step of stirring the magnetic solid phase and the washing water by moving the magnet away from the cuvette wall;
(2) Injecting a detection reagent for detecting the amount of antigen (or antibody) bound to the magnetic solid phase after washing into the magnetic solid phase;
A detection reagent injection and a stirring position for performing the step of stirring the injected detection reagent and the magnetic solid phase are provided.

  The B / F separation table includes a step of stirring the magnetic solid phase and the washing water by moving the magnet away from the cuvette wall, a step of collecting the magnetic solid phase on the cuvette wall by the magnet, It is characterized in that it is performed at a position different from the step of sucking and separating the waste liquid and the step of injecting cleaning water.

  In addition, the B / F separation table detects the amount of antigen (or antibody) bound to the magnetic solid phase in the washed magnetic solid phase in the step of stirring the injected detection reagent and the magnetic solid phase. It is characterized in that it is carried out at a position different from the step of injecting the detection reagent for this purpose.

  The B / F separation table is characterized in that a position for preliminarily collecting the solid phase is provided before the first B / F separation position.

  Further, the agitation at the B / F separation table is characterized in that it is performed by rotating the cuvette between two elastic bodies.

  In addition, a second cuvette transfer means for transferring the cuvette from the B / F separation table to the position of the detector is provided.

According to the automatic analyzer of the present invention,
(1) a cuvette for reacting a sample with a reagent;
(2) a sample dispensing means and a reagent dispensing means for respectively dispensing a sample and a reagent into the cuvette;
(3) a plurality of the cuvettes in the circumferential direction, and a reaction turntable that causes the sample and the reagent to undergo an antigen-antibody reaction while rotating;
(4) The released antigen (or antibody) F contained in the cuvette after the antigen-antibody reaction is separated from the antigen (or antibody) B bound to the magnetic solid phase, and after the B / F separation, the magnetic solid A B / F separation table for injecting and reacting a detection reagent that reacts with an antigen (or antibody) bound to a phase;
(5) a detector for quantifying the reacted detection reagent,
Between the reaction turntable and the B / F separation table, there is a first cuvette transfer means for transferring the cuvette from the reaction turntable to the B / F separation table.
It has become possible to provide an automatic analyzer that can simplify the operation of a plurality of nozzles, magnets, and stirring mechanisms used for B / F separation.

  Embodiments of the present invention will be described below with reference to the drawings.

  FIG. 2 shows an embodiment of a new automatic analyzer according to the present invention. In the following, the linkage between the apparatus and the reaction / detection process is described for the one-step reaction and the two-step reaction. The reaction time until B / F separation differs for each measurement item, and the timing of B / F separation is different. In the present invention, the B / F separation is completed by reacting several times on the reaction turntable and carrying the cuvette at the timing for performing the B / F separation to the B / F separation table. It becomes easy to cope.

<< In the case of one-step reaction / detection process >>
(1) A sample is dispensed from the sample turntable 12 into a reaction tube (hereinafter referred to as a cuvette) arranged on the reaction turntable 11.

  (2) Reagent 1 is dispensed from the reagent 1 turntable 13.

  (3) Reagent 2 is dispensed from the reagent 2 turntable 14.

  (4) After the reaction, the cuvette is transferred to the B / F separation table 16 (B / F separation device) by the cuvette transfer hand 15 and B / F separation is performed.

  (5) The cuvette is transferred from the B / F separation table 16 to the light emission detector 17 by the cuvette transfer hand 15, and the light emission amount is measured.

  (6) The cuvette is transferred from the light emission detector 17 to the cuvette dust 18 by the cuvette transfer hand 15, and the cuvette is discarded.

<< In the case of two-step reaction / detection process >>
(1) A sample is dispensed from the sample turntable 12 into a reaction tube (hereinafter referred to as a cuvette) arranged on the reaction turntable 11.

  (2) Reagent 1 is dispensed from the reagent 1 turntable 13.

  (3) After the reaction, the cuvette is transferred to the B / F separation table 16 (B / F separation device) by the cuvette transfer hand 15 and B / F separation is performed.

  (4) The cuvette is transferred from the B / F separation table 16 to the reaction turntable 11 with the cuvette transfer hand 15, and the reagent 2 is dispensed from the reagent 2 turntable 14.

  (5) After the reaction, the cuvette is transferred to the B / F separation table 16 (B / F separation device) by the cuvette transfer hand 15 and B / F separation is performed.

  (6) The cuvette is transferred from the B / F separation table 16 to the light emission detector 17 by the cuvette transfer hand 15, and the light emission amount is measured.

  (7) The cuvette is transferred from the light emission detector 17 to the cuvette dust 18 by the cuvette transfer hand 15, and the cuvette is discarded.

  3 and 4 show a B / F separation device according to the present invention. The number of times of B / F separation, the number of magnets, nozzles, and stirring positions shown in the figure is merely an example, and is not limited to the number described in the figure.

  A cuvette 21 is arranged in a collar 28 on the circumference of the cuvette transfer table 26. Three magnets 22 that can move back and forth in the lateral direction of the cuvette 21 are disposed at the position where the B / F separation is performed. The three magnets move back and forth with one motor.

  Above the B / F separation position where the magnet 22 is disposed, a suction nozzle 23 and an injection nozzle 24 for injecting a cleaning liquid, or a luminescence auxiliary reagent A liquid injection nozzle 25 for injecting a luminescence auxiliary reagent A liquid are provided. The nozzle is fixed to one plate or the like and moved up and down by one motor.

  Under the B / F separation position where the magnet 22 is disposed, a stirring base 27 is provided so that three stirring bases can be rotated by one motor using gears or the like.

  It should be noted that what is indicated by the numbers in FIGS. 3 and 4 is organized.

  21: Cuvette-reaction tube containing reaction solution.

  22: The magnetic beads in the magnet-cuvette are assembled on the wall surface. When assembling the magnetic beads, it approaches the side surface of the cuvette and moves away from the cuvette when dispensing and stirring the cleaning liquid, so that it can move back and forth.

  23: Suction nozzle-sucks waste liquid in the cuvette. It is fixed together with the injection nozzle and can move up and down.

  24: Injection nozzle—injects the cleaning liquid into the cuvette. It is fixed with the suction nozzle and can move up and down.

  25: Luminescence auxiliary reagent A liquid injection nozzle—Injects the luminescence auxiliary reagent A liquid into the cuvette. It is fixed with the suction nozzle and can move up and down.

  26: Cuvette transfer table-Holds the cuvette around and sends the cuvette to the next port by rotating operation.

  27: Stirring table-A table provided with a sponge cut out so as to grip the cuvette. By rotating, the solution in the cuvette is rotated and stirred.

  28: Color-A cuvette is made of a material softer than the cuvette so that the cuvette surface is less likely to be scratched, and the contact surface with the cuvette is made smaller. As a modification, it is also possible to substitute by a system in which the cuvette is sandwiched between elastic members having a shape in which the cuvette is sandwiched between two leaf springs arranged in parallel.

  The operation of the B / F separator according to the present invention is as follows.

  (1) The turntable 26 rotates to transfer the cuvette 21, and the magnet 22 approaches the side surface of the cuvette at the B / F separation position.

  (2) After the waste liquid in the cuvette is sucked by the suction nozzle 23, the magnet 22 is separated from the cuvette 21.

  (3) The cleaning liquid is injected into the cuvette by the injection nozzle 24.

  (4) The stirring base 26 grips and rotates the bottom of the cuvette 21 to clean the inner wall of the cuvette and the surface of the magnetic beads.

  (5) Returning to (1), the same process is repeated twice.

  (6) There are three B / F separation positions in the example of FIG. 4, and the cuvette 21 is stepped forward and advanced one by one. Therefore, in the cuvette where the B / F separation is the third time, Instead of the cleaning liquid, the light emission auxiliary reagent A liquid is injected from the light emission auxiliary reagent A liquid injection nozzle 25.

  In this embodiment, the B / F separation is performed at three positions, positions 3, 4, and 5 as shown in the above description and FIG. 5, and four processes are performed at each position. . FIG. 6 illustrates the time required for the four steps.

  First, in the first step, the magnetic beads are gathered by bringing the magnet close to the side wall of the cuvette. This process takes 5 seconds.

  Next, in the second step, the waste liquid in the cuvette is sucked and removed by the suction nozzle while the magnetic beads are gathered on the side wall of the cuvette by the magnet. This process takes 3 seconds.

  Next, in the third step, the magnet is moved away from the side wall of the cuvette, and cleaning water is injected into the cuvette from the injection nozzle. This process takes 1 second.

  Finally, in the fourth step, the magnetic beads are stirred and washed by rotating the stirring table. This process takes 5 seconds.

  These required times are those realized by a small and light magnet (Japanese Patent Laid-Open No. 2006-218442), and the conventional magnet takes a separation time nearly twice as long. After all, in this embodiment, it takes about 14 seconds to perform the B / F separation operation at one B / F separation position (cuvette stop position).

  In the B / F separation process, the first B / F separation process takes the longest time among the B / F separation processes using the magnet repeated three times. This is because, in the first B / F separation, the sample contains a large amount of protein, so the sample is viscous, and it takes time to attract with the magnet. Therefore, in order to perform the B / F separation efficiently in a short time, one point is how efficiently the first stage process (attraction by the first magnet) is performed.

  Therefore, in order to increase the initial B / F separation efficiency, the solid phase preliminary assembly may be performed in advance by a separately prepared magnet at position 2 of the B / F separation table in FIG. Thereby, the initial B / F separation efficiency can be increased.

  In this embodiment, the cuvette 21 is held on the turntable 26 and rotated and transferred. However, the cuvette 21 may be held and transferred on a linear reaction table as shown in FIG. .

  As a result of this embodiment, a dedicated table for performing B / F separation can be provided, and the B / F separation portion and the reaction portion can be separated, and B / F separation can be performed by repeating simple operations. It became so.

  As a result, the plurality of magnets, the plurality of nozzles, and the plurality of stirring tables can be moved with one power, respectively, and the movable shaft is reduced, thereby reducing the cost.

  In addition, since the B / F separation is completed simply by transferring the cuvette at the timing for performing the B / F separation to the B / F separation unit, the biochemical analysis method can be easily diversified.

  In addition, since the luminescent auxiliary reagent A liquid injection nozzle is disposed at the end of the B / F separation, the power required to move the nozzle required for reagent dispensing up and down is unnecessary, and the cost is reduced.

  In the B / F separation device of Example 1, since the four separation steps were processed at one location of the cuvette stop position, a long stop time was required. This stop time is 15 to 30 seconds, and 120 to 240 samples / hour in terms of throughput.

  When the cycle time is shortened from 15 to 30 seconds / sample to 9 seconds / sample, the throughput of the B / F separation apparatus increases from 240 samples / hour to 400 samples / hour. Therefore, the second embodiment is devised to increase the number of transfer steps of the B / F separation device, to reduce the waiting time at each position, and to reduce the waiting time (cycle time) at each position. .

  In the present embodiment, the four steps performed at one stop position in the first embodiment are divided into two, that is, the previous steps 1 to 3 and the subsequent step 4, and are executed in parallel at two positions. FIG. 8 shows the B / F separation process divided into two. Pre-steps 1 to 3 are assembly of magnetic beads, waste water suction and washing water injection, and post-step 4 consists of resuspension. The pre-processes 1 to 3 and the post-process 4 are combined to form one B / F separation.

  FIG. 9 shows an embodiment of the B / F separation device. While the stop positions of the B / F separation device of Example 1 were 8 positions in total, this example increased by 2 to 10 positions. That is, in FIG. 9, the positions 3 and 4 in FIG. 5 are divided into two parts, a pre-process and a post-process, respectively, and positions 4 and 6 are newly added. Further, the agitation at position 5 in FIG. 5 is replaced with high-speed agitation at position 8 in FIG.

  Based on FIG. 9, the operation of the present B / F separation device will be described. In the figure, position 1 is a cuvette attaching / detaching position. The cuvette is initially set to position 1 and goes around the turntable starting from this position.

  During this time, the collection of magnetic beads corresponding to the preceding steps 1 to 3 shown in FIG. In addition, re-suspension of the post-process is performed at positions 4 and 6. Stirring after injection of the luminescence auxiliary reagent A solution at position 7 is also performed by high-speed stirring at position 8.

  While the cuvette goes around the turntable, B / F separation is performed once at each of positions 3, 4, 5, 6, and 7, and a total of 3 times. As a result, the pre-process and the post-process can be performed in parallel, and the total required time can be shortened.

  Note that this embodiment can be modified. That is, in this embodiment, the cuvette is held on the turntable and rotated around the circumference. However, the cuvette may be held on the linear reaction table and transferred by analogy with FIG. good.

  In addition, the process is not divided as shown in FIG. 8, but as shown in FIG. 10, the process is divided into a pre-process and a post-process depending on whether or not a magnet is used for B / F separation. May be.

  In the B / F separation step, the first B / F separation step takes the longest time among the B / F separation steps by the magnet repeated three times. This is because, in the first B / F separation, the sample contains a large amount of protein, so the sample is viscous, and it takes time to attract with the magnet. Therefore, in order to perform the B / F separation efficiently in a short time, one point is how efficiently the first stage process (attraction by the first magnet) is performed.

  Therefore, in order to improve the initial B / F separation efficiency, a solid phase preliminary assembly may be performed in advance by a separately prepared magnet at position 2 of the B / F separation table in FIG. Thereby, the initial B / F separation efficiency can be increased.

  After all, in this embodiment, the cuvette stop position is increased from 8 positions to 10 positions, and the B / F separation position, which is particularly time-consuming, is divided into two. As a result, the time during which the cuvette is stopped at one position has been shortened from 15 seconds to 9 seconds, so that the final throughput is increased to 240 samples / despite the increase in cuvette stop positions from 8 to 10. It was possible to improve from time to 400 samples / hour.

  A modification of the collar 28 in FIGS. 3 and 4 will be described. FIG. 11 exemplifies a system in which the cuvette is sandwiched by elastic members having a shape in which the cuvette is sandwiched between two leaf springs arranged in parallel.

  This is a cuvette that is more durable than a soft collar by sandwiching the cuvette with two leaf springs that are processed to open angles at both ends and have a spatial bulge that allows the cuvette to be sandwiched in the center. It is intended to provide a stirring mechanism.

  The cuvette is transported to the central bulge portion from the left open ends of the two leaf springs together with the rotation of the B / F separation table, and is fixed by being sandwiched between the leaf springs. After fixing, the sample in the cuvette is agitated by rotating the leaf spring together with the cuvette with a motor capable of controlling the rotation angle.

  Since the rotation angle of the motor can be controlled, the direction of the two leaf springs can be stopped toward the moving direction of the cuvette at the end of stirring. As a result, the cuvette is transported toward the right open end of the two leaf springs along with the rotation of the B / F separation table, and can move to the next position of the B / F separation table.

  Such a stirring mechanism using a leaf spring is provided at positions 3, 4, 5 in FIG. 5 and positions 4, 6 in FIG. 9, respectively.

  Can be widely used for automatic analyzers.

It is a figure which shows the measurement principle of EIA method. It is a figure which shows one Example of the automatic analyzer concerning this invention. It is a side view which shows one Example of the B / F separation apparatus concerning this invention. It is a top view which shows one Example of the B / F separation apparatus concerning this invention. It is a plane process figure which shows one Example of the B / F separation apparatus concerning this invention. It is a figure which shows the required time of the B / F separation process concerning this invention. It is a figure which shows the modification of the B / F separation apparatus concerning this invention. It is a figure which shows the required time of the B / F separation process concerning this invention. It is a plane process figure which shows another Example of the B / F separation apparatus concerning this invention. It is another figure which shows the required time of the B / F separation process concerning this invention. It is a figure which shows one Example of the stirring mechanism concerning this invention.

Explanation of symbols

1: solid phase (bead solid phase), 2: solid phase antibody, 3: unbound antigen to solid phase antibody, 4: antigen-antibody complex, 5: antigen bound to solid phase antibody, 6: antigen-antibody Enzyme-labeled antibody not bound to the complex, 7: labeled enzyme, 8: enzyme-labeled antibody-antigen-antibody complex, 9: enzyme-labeled antibody bound to the antigen-antibody complex, 10: coloring reagent (before color development), 10 ^' : Coloring reagent (after color development), 11: Reaction turntable, 12: Sample turntable, 13: Reagent 1 turntable, 14: Reagent 2 turntable, 15: Cuvette transfer hand, 16: B / F separation table, 17: luminescence detector, 18: cuvette dust, 21: cuvette, 22: magnet, 23: suction nozzle, 24: injection nozzle, 25: luminescence auxiliary reagent A liquid injection nozzle, 26: cuvette transfer table, 27: stirring table, 28: Color

Claims (7)

(1) a cuvette for reacting a sample with a reagent;
(2) a sample dispensing means and a reagent dispensing means for respectively dispensing a sample and a reagent into the cuvette;
(3) a plurality of the cuvettes in the circumferential direction, and a reaction turntable that causes the sample and the reagent to undergo an antigen-antibody reaction while rotating;
(4) The released antigen (or antibody) F contained in the cuvette after the antigen-antibody reaction is separated from the antigen (or antibody) B bound to the magnetic solid phase, and after the B / F separation, the magnetic solid A B / F separation table for injecting and reacting a detection reagent that reacts with an antigen (or antibody) bound to a phase;
(5) a detector for quantifying the reacted detection reagent,
An automatic analysis is characterized in that a first cuvette transfer means for transferring the cuvette from the reaction turntable to the B / F separation table exists between the reaction turntable and the B / F separation table. apparatus. The B / F separation table is
(1) collecting the magnetic solid phase on the cuvette wall with a magnet;
A step of sucking and separating the waste liquid from the assembled magnetic solid phase;
Injecting cleaning water;
A plurality of B / F separation positions for performing a step of stirring the magnetic solid phase and the washing water by moving the magnet away from the cuvette wall;
(2) Injecting a detection reagent for detecting the amount of antigen (or antibody) bound to the magnetic solid phase after washing into the magnetic solid phase;
The automatic analyzer according to claim 1, further comprising a detection reagent injection and a stirring position for performing the step of stirring the injected detection reagent and the magnetic solid phase. The B / F separation table includes a step of stirring the magnetic solid phase and the washing water by moving the magnet away from the cuvette wall, a step of collecting the magnetic solid phase on the cuvette wall by the magnet, and a waste liquid from the assembled magnetic solid phase. 3. The automatic analyzer according to claim 2, wherein the automatic analyzer is different from the step of sucking and separating and the step of injecting washing water. The B / F separation table is a step for stirring the injected detection reagent and the magnetic solid phase to detect the amount of the antigen (or antibody) bound to the magnetic solid phase after the washing. 3. The automatic analyzer according to claim 2, wherein the automatic analyzer is performed at a position different from the step of injecting the detection reagent. The automatic analyzer according to claim 2, wherein a position for preliminarily collecting the solid phase is provided before the first B / F separation position in the B / F separation table. 3. The automatic analyzer according to claim 2, wherein the agitation at the B / F separation table is performed by rotating the cuvette between two elastic bodies. 2. The automatic analyzer according to claim 1, further comprising second cuvette transfer means for transferring the cuvette from the B / F separation table to the position of the detector.

Images