CN111381054A - Magnetic separation device, sample analyzer, and flow type fluorescence immunoassay analyzer - Google Patents

Magnetic separation device, sample analyzer, and flow type fluorescence immunoassay analyzer Download PDF

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Publication number
CN111381054A
CN111381054A CN201811642288.7A CN201811642288A CN111381054A CN 111381054 A CN111381054 A CN 111381054A CN 201811642288 A CN201811642288 A CN 201811642288A CN 111381054 A CN111381054 A CN 111381054A
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CN
China
Prior art keywords
magnetic
turntable
base
sample container
separation device
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Pending
Application number
CN201811642288.7A
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Chinese (zh)
Inventor
王锐
周宇航
刘治志
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Shenzhen Dymind Biotechnology Co Ltd
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Shenzhen Dymind Biotechnology Co Ltd
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Priority to CN201811642288.7A priority Critical patent/CN111381054A/en
Publication of CN111381054A publication Critical patent/CN111381054A/en
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements

Abstract

The application discloses magnetic separation device, it includes: a base provided with an accommodating groove; the rotating disc is rotatably arranged in the accommodating groove, and at least one accommodating hole is formed in the rotating disc and is used for accommodating a sample container filled with a sample and/or a magnetic compound; the magnetic pieces correspond to the accommodating holes in quantity one to one, and the magnetic pieces are detachably arranged on the base or the turntable and are used for adsorbing the magnetic compound in the sample container on the inner wall of the sample container; and the push-pull mechanism is arranged on the base or the turntable and is used for drawing the magnetic part corresponding to the accommodating hole where the sample container is placed out of the base or the turntable when the sample container rotates to the detection station on the base along with the turntable. Through the mode, the adsorption effect of the magnetic compound can be improved, and the design of the detection station can be facilitated.

Description

Magnetic separation device, sample analyzer, and flow type fluorescence immunoassay analyzer
Technical Field
The invention relates to the technical field of medical equipment, in particular to a magnetic separation device, a sample analyzer and a flow type fluorescence immunoassay analyzer.
Background
Currently, in a magnetic separation device of a medical apparatus, for example, a magnetic separation device of an immunoassay analyzer, the purpose of washing and magnetic separation is achieved by adsorbing magnetic compounds (e.g., magnetic beads) on the inner wall of a sample container and then sucking up supernatant in the sample container. In this process, the magnetic bead adsorbs the absorption effect on sample container inner wall can influence the loss of magnetic bead in the cleaning process, and the magnetic bead loss can influence the accuracy of testing data. How to reduce the loss of magnetic beads and how to improve the adsorption effect of magnetic beads, thereby improving the detection accuracy, has become a focus of attention of various manufacturers.
Disclosure of Invention
The main technical problem who solves of this application provides a magnetic separation device, sample analysis appearance and STREAMING fluorescence immunoassay appearance, can improve the adsorption effect of magnetic composite and can reduce the magnetic composite quantity of being siphoned away by the imbibition needle simultaneously, and can be convenient for detect the design of station.
In order to solve the above technical problem, one technical solution adopted in the embodiments of the present application is: provided is a magnetic separation device including: a base provided with an accommodating groove; the rotating disc is rotatably arranged in the accommodating groove, and at least one accommodating hole is formed in the rotating disc and is used for accommodating a sample container filled with a sample and/or a magnetic compound; the magnetic pieces correspond to the accommodating holes in quantity one to one, and the magnetic pieces are detachably arranged on the base or the turntable and are used for adsorbing the magnetic compound in the sample container on the inner wall of the sample container; and the push-pull mechanism is arranged on the base or the turntable and is used for drawing the magnetic part corresponding to the accommodating hole where the sample container is placed away from the base or the turntable when the sample container rotates to the detection station on the base along with the turntable, so that the detection device can conveniently suck the magnetic compound in the sample container.
In order to solve the above technical problem, another technical solution adopted in the embodiment of the present application is: there is provided a sample analyser comprising a magnetic separation device as described above.
In order to solve the above technical problem, another technical solution adopted in the embodiment of the present application is: the flow type fluorescence immunoassay analyzer comprises a detection device and the magnetic separation device, wherein the detection device comprises a flow chamber, and the detection device is used for sucking a target detection object in a sample container of the magnetic separation device into the flow chamber for optical detection.
This application embodiment includes through setting up magnetic separation device: a base provided with an accommodating groove; the rotating disc is rotatably arranged in the accommodating groove, and at least one accommodating hole is formed in the rotating disc and is used for accommodating a sample container filled with a sample and/or a magnetic compound; the magnetic pieces correspond to the accommodating holes in quantity one to one, and the magnetic pieces are detachably arranged on the base or the turntable and are used for adsorbing the magnetic compound in the sample container on the inner wall of the sample container; the push-pull mechanism is arranged on the base or the turntable and used for drawing the magnetic part corresponding to the accommodating hole where the sample container is placed away from the base or the turntable when the sample container rotates to the detection station on the base along with the turntable so as to facilitate the detection device to suck the magnetic compound in the sample container; can promote magnetic composite's adsorption effect, can reduce the magnetic composite quantity that is siphoned away by the imbibition needle simultaneously and can be convenient for detect the design of station.
Drawings
FIG. 1 is a schematic structural diagram of a magnetic separation apparatus according to an embodiment of the present application;
FIG. 2 is a schematic structural diagram of a first embodiment of a magnetic element layout according to an example of the present application;
FIG. 3 is a schematic diagram showing the position relationship between the detecting device and the magnetic separating device according to the embodiment of the present application;
FIG. 4 is a schematic top view of a first embodiment of a magnetic element layout according to the present application;
FIG. 5 is a schematic diagram illustrating a positional relationship among the magnetic members, the turntable, and the accommodating hole according to the first embodiment of the magnetic member layout of the present application;
FIG. 6 is a schematic view of the magnetic attraction principle of the first embodiment of the magnetic element layout of the present application;
FIG. 7 is a schematic view of a push-pull mechanism according to a first embodiment of the magnetic element layout of the present application;
FIG. 8 is a schematic top view of a magnetic separation apparatus according to a second magnetic arrangement of the present application;
FIG. 9 is a schematic top view of a magnetic separation apparatus according to a third magnetic element layout of the present application;
FIG. 10 is a schematic view of a magnetic attraction principle according to a third magnetic element layout of the present application;
fig. 11 is a schematic view of another magnetization method and an adsorption principle of the magnetic member according to the embodiment of the present application.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. It is to be understood that the specific embodiments described herein are merely illustrative of the application and are not limiting of the application. It should be further noted that, for the convenience of description, only some of the structures related to the present application are shown in the drawings, not all of the structures. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The terms "first", "second", etc. in this application are used to distinguish between different objects and not to describe a particular order. Furthermore, the terms "include" and "have," as well as any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
Referring to fig. 1 and 2, fig. 1 is a schematic structural diagram of a magnetic separation device according to an embodiment of the present application. Fig. 2 is a schematic structural diagram of a first implementation manner of a magnetic member layout according to an embodiment of the present application.
In the present embodiment, the magnetic separation apparatus includes a base 10, a turntable 11, and a magnetic member 12.
The base 10 is provided with a receiving groove.
The rotating disc 11 is rotatably disposed in the accommodating groove, and the rotating disc 11 is provided with at least one accommodating hole a for accommodating a sample container b containing a sample and/or a magnetic composite.
Alternatively, the turntable 11 is cylindrical, and each receiving hole a is equidistant from the axis of rotation of the turntable 11. The receiving hole a is provided adjacent to an edge position of the turntable 11, i.e., in an edge area of the turntable 11. The edge region refers to a position closer to the edge of the turntable 11 with respect to the center of the turntable 11 and the edge of the turntable 11. In this way, on the one hand, more accommodating holes a can be arranged on the same-size turntable 11 to accommodate the sample containers b; on the other hand, the accommodating hole a can be closer to the magnetic member 12 on the base 10, so as to improve the adsorption effect of the magnetic member 12 on the magnetic compound. The plurality of receiving holes a may be annularly distributed and equally spaced apart.
The magnetic member 12 is detachably disposed on the base 10 or the turntable 11, and is used for, when the sample container B rotates with the turntable 11 to the detection station J on the base 10, detaching the magnetic member 12 corresponding to the receiving hole a where the sample container B is placed from the base 10 or the turntable 11, so that the detection device can conveniently suck the magnetic compound in the sample container B.
The magnetic compound can comprise a magnetic sphere, an antigen or an antibody positioned on the surface of the magnetic sphere, and an analyte in blood combined with the antigen or the antibody. The surface of the magnetic ball is modified to have a coating structure and also has a functional group, the functional group is combined with antigen or antibody, the antigen or antibody is combined with a substance to be detected in blood to gradually form a large immune complex, and the final immune complex (namely, a target detection substance) is obtained by magnetic separation, separation and cleaning and is sent into a flow chamber of a detection device along with sheath fluid to be detected. During magnetic separation and cleaning, the immune complex is adsorbed on the inner wall of the sample container, supernatant is sucked away, the immune complex adsorbed on the inner wall is released at a detection station, and the immune complex is sucked into a flow chamber of the detection device for optical detection.
It will be appreciated that the magnetic complex may be either a reaction substrate prior to reaction: for example, the capture antibody-coated magnetic bead mixture may be a magnetic bead target detection substance formed after the reaction. In addition, it will be appreciated that the sample container may contain, in addition to the sample and/or magnetic complexes, other substances involved in the reaction, such as: reagents, ligands, diluents, and the like.
Referring to fig. 3 in conjunction with fig. 1 and fig. 2, fig. 3 is a schematic diagram illustrating a position relationship between a detecting device and a magnetic separating device according to an embodiment of the present application. The sample suction needle 41 of the detection device 40 is movable or rotatable to the detection station J, and is extendable into the sample container b to suck the target detection object in the sample container b of the detection station J. Optionally, the magnetic separation device further includes a blending mechanism disposed at the detection station J, and the blending mechanism is configured to blend the magnetic compound and the liquid in the sample container b that rotates to the detection station J along with the turntable 11. Alternatively, the mixing mechanism may be the sample sucking needle 41 of the detection device 40. The detection device 40 controls the discharge or suction of the sample from the sample suction needle 41 to mix the sample, i.e., to suck and discharge the sample. Of course, in other embodiments, a blending mechanism may be separately disposed adjacent to the detection station J, and the embodiment of the present application is not limited to the manner of blending by using the sample suction needle 41 of the detection device 40.
For example, the kneading mechanism is a stirring rod 42 provided in the detection device 40, and the sample is stirred and kneaded by the stirring rod 42.
Optionally, at least one pipetting station X is provided on the base 10, the magnetism generated by the magnetic member 12 being progressively increased from the detection station J to the pipetting station X.
The magnetic separation device can further comprise a first bracket 13, a light emitter 14, a light receiver 15, a shielding piece 16, a cleaning container 17, a liquid suction assembly 18, a liquid adding assembly 19, a support rod 20, a fixed connection seat 31 and a sample container detection assembly 32. Wherein the pipetting assembly 18 is positioned at the pipetting station X. The light emitter 14 and the light receiver 15 are both fixed on the base 10 through the first bracket 13, and the light emitter 14 and the light receiver 15 are oppositely arranged and spaced. The shielding member 16 is fixed on the turntable 11, and when the shielding member 16 rotates with the turntable 11 to a position corresponding to the light emitter 14 and the light receiver 15, the shielding member 16 is partially located between the light emitter 14 and the light receiver 15 to shield the light emitted by the light emitter 14 toward the light receiver 15.
For example, when the shielding member 16 rotates with the turntable 11 to a position corresponding to the light emitter 14 and the light receiver 15, the light emitted by the light emitter 14 toward the light receiver 15 is shielded by the shielding member 16, and the light emitted by the light emitter 14 cannot be received by the light receiver 15; when the shutter 16 is not in the position corresponding to the light emitter 14 and the light receiver 15, the light receiver 15 can receive the light emitted by the light emitter 14, so that the magnetic separation device can determine the initial position of rotation of the dial 11 by whether the light receiver 15 can receive the light emitted by the light emitter 14.
In this embodiment, the cleaning container 17 is fixed on the rotating disc 11 at a position corresponding to the shielding member 16 and is inserted into the avoiding hole of the shielding member 16. By arranging the cleaning container 17 at the position corresponding to the shielding piece 16, the magnetic separation device is compact in structure and convenient for miniaturization design, and by arranging the avoiding hole on the shielding piece 16, the cleaning container 17 is allowed to pass through to be exposed, so that the cleaning of the liquid suction assembly 18 or the liquid feeding assembly 19 is not influenced. In another embodiment, the cleaning vessel 17 can be fixed to the screen 16. By fixing the cleaning vessel 17 to the shutter 16, a structure for fixing the cleaning vessel 17 is not additionally provided, so that the structure of the magnetic separation apparatus is made relatively simple.
A pipetting assembly 18 is secured to the base 10 and is used to aspirate liquid in the sample container b which rotates with the turntable 11 to the position where the pipetting assembly 18 is located. Specifically, pipetting assembly 18 includes a second mount 181, a third mount 182, a pipette needle 183, and a wash needle 184. The second bracket 181 is fixed to the base 10, and the third bracket 182 is movably disposed on the second bracket 182 in a direction parallel to the rotation axis of the turntable 11 to approach or separate from the turntable 11. The pipette needle 183 and the wash needle 184 are fixed to the third frame 182, and the wash needle 184 is shorter than the pipette needle 183 so that the outer wall of the pipette needle 183 can be washed when the wash needle 184 discharges liquid.
Specifically, the length of the liquid suction needle 183 is greater than that of the cleaning needle 184, the liquid suction needle 183 and the cleaning needle 184 are fixed in relative position and are disposed to abut against each other, and the height of the liquid outlet of the cleaning needle 184 relative to the base 10 is greater than the height of the liquid suction port of the liquid suction needle 183 relative to the base 10.
When the sample container b rotates to the lower part of the liquid suction needle 183 and the cleaning needle 184 along with the turntable 11, the magnetic separation device controls the liquid suction needle 183 and the cleaning needle 184 to extend into the sample container b for liquid suction.
The cleaning vessel 17 is used for cleaning the pipette needle 183 of the pipette assembly 18 when the turntable 11 is rotated to the position of the pipette assembly 18. Specifically, when the cleaning container 17 rotates to the position below the liquid suction needle 183 and the cleaning needle 184 along with the turntable 11, the magnetic separation device controls the liquid suction needle 183 and the cleaning needle 184 to move downwards and extend into the cleaning container 17, the cleaning needle 184 and the liquid suction needle 183 discharge liquid at the same time, the liquid discharged by the cleaning needle 184 cleans the outer wall of the liquid suction needle 183, and the liquid discharged by the liquid suction needle 183 cleans the inner wall of the liquid suction needle 183.
The priming assembly 19 is fixed to the base 10 and is used to inject liquid into the sample container b which rotates with the turntable 11 to the position where the priming assembly 19 is located. The injected liquid may be a reagent. The filling assembly 19 is located at a filling station T on the base 10. Optionally, the magnetism of the liquid adding station T is weaker than that of the liquid absorbing station X, and the magnetism of the liquid adding station T is stronger than that of the detection station J.
Optionally, the priming assembly 19 includes a fourth cradle 191, a fifth cradle 192, and a priming needle 193. The fourth bracket 191 is fixed to the base 10, and the fifth bracket 192 is movably disposed on the fourth bracket 191 in a direction parallel to the rotation axis of the turntable 11 to approach or separate from the turntable 11. The filling needle 193 is fixed to the fifth bracket 192. The fifth bracket 192 may be fixedly disposed on the fourth bracket 191, so that the filling needle 19 cannot move up and down, thereby saving the cost of designing a driving mechanism for moving up and down.
It should be understood that the filling needle 193 may be fixedly disposed with respect to the base 10 so as not to move up and down, and the cleaning container 17 may be used only for cleaning the pipette needle 183.
In another embodiment, when the sample container b rotates with the turntable 11 to a position below the liquid adding needle 193, the magnetic separation device controls the liquid adding needle 193 to move downwards to extend into the sample container b, and then the liquid adding needle 193 discharges liquid to add liquid into the sample container b.
The cleaning vessel 17 is further used to clean the refill unit 19 when it is rotated with the turntable 11 to the position of the refill unit 19. Specifically, when the cleaning container 17 rotates with the turntable 11 to a position below the liquid feeding needle 193, the magnetic separation device controls the liquid feeding needle 193 to move downward, and the liquid feeding needle 193 is inserted into the cleaning container 17 to be cleaned.
The fixed connection holder 31 is connected to the base 10 through the support bar 20. Alternatively, the number of the support rods 20 is four. In other embodiments, the number of the support rods 20 may be three. The fixed connection seat 31 is used for fixing with other structures.
The sample container testing assembly 32 includes a sixth rack 321 and a test sensor 322 disposed on the sixth rack 321. The detection sensor 322 is configured to detect whether or not a sample container b is placed in a certain receiving hole b when the receiving hole b is rotated to a position corresponding to the detection sensor 322. Alternatively, the detection sensor may be an optical coupler, and specifically may be a reflective optical coupler.
Next, a structure for realizing the push-pull mechanism in the embodiment of the layout of the first magnetic material will be described.
Referring to fig. 4-7, fig. 4 is a schematic top view of a first embodiment of a magnetic element layout according to the present application. Fig. 5 is a schematic diagram of a positional relationship among the magnetic member, the turntable, and the accommodating hole according to the first embodiment of the magnetic member layout of the present application. FIG. 6 is a schematic view of the magnetic attraction principle of the first embodiment of the magnetic member layout of the present application. Fig. 7 is a schematic structural diagram of a push-pull mechanism according to a first embodiment of the magnetic member layout of the present application.
In the first embodiment, the magnetic member 12 is provided on the base 10. The number of the magnetic members 12 is plural, and the number of the magnetic members 12 corresponds to the number of the accommodation holes a. The magnetic member 12(a) at the detection station J is removably disposed in the corresponding mounting hole of the base 10, and other magnetic members 12 not at the detection station J may be fixed in the corresponding mounting holes of the base 10.
It should be understood that the number of the detection stations J may be multiple, and when the number of the detection stations J is multiple, the magnetic members 12 at the multiple detection stations J are all detachably disposed in the corresponding mounting holes on the base 10.
In this embodiment, the first specific way of increasing the magnetism generated by the magnetic member 12 from the detection station J to the liquid suction station X is: the area of the surface of each magnetic member 12 close to one side of the rotary table 11 from the detection station J to the liquid suction station X is gradually increased; the second specific implementation mode is as follows: the thickness of each magnetic member 12 gradually increases from the inspection station J to the pipetting station X. It will be appreciated that in other embodiments, the gradual increase in magnetism from the detection station J to the pipetting station X can also be achieved in combination with the first and second implementations, for example, the respective magnetic members 12 increase in area and thickness from the detection station J to the pipetting station X.
Alternatively, the magnetic member 12 is disposed adjacent to an edge of the turntable 11, the base 10 is provided with a first notch q1 at a position corresponding to the magnetic member 12, and the magnetic member 12 is exposed toward the turntable 11 through the first notch q 1. In this way, the magnetic member 12 is exposed toward the side of the rotation axis of the turntable 11, and the adsorption effect of the magnetic member 12 on the magnetic complex in the sample container b is increased.
Alternatively, the turntable 11 is provided with a second notch q2 at a position corresponding to the receiving hole a, and the sample container b placed in the receiving hole a is exposed to the base 10 through the second notch q 2.
In this way, the sample container b placed in the accommodating hole a is exposed to the base 10 through the second notch q2, so as to increase the adsorption effect of the magnetic member 12 on the magnetic compound in the sample container b.
As shown in fig. 5, the surface of the magnetic member 12 close to the receiving hole a is a plane, the receiving hole a is a circular hole, and the surface of the magnetic member 12 close to the receiving hole a is perpendicular to a reference plane ABCD defined by the rotation axis AB of the turntable 11 and the axis CD of the receiving hole a.
Optionally, the length of the magnetic member 12 is equal to the depth of the receiving hole a, so that the inner wall of the portion of the sample container b located in the receiving hole a can adsorb magnetic compounds at different positions in the height direction, thereby improving the adsorption efficiency. The upper end of the magnetic member 12 may be flush with the upper end of the receiving hole a, and the lower end of the magnetic member 12 may be flush with the bottom of the receiving hole a.
Alternatively, the shape of the magnetic member 12 may be a rectangular parallelepiped, i.e., each surface of the magnetic member 12 is a plane. In other embodiments, only the surface of the magnetic member 12 near the accommodating hole a may be provided as a flat surface.
Because the shape of the magnetic part 12 is a cuboid, and the shape of the used magnetic part 12 is a plate type, the processing difficulty is small, the cost can be effectively reduced, and the required effect can be achieved.
As shown in fig. 7, the magnetic separation device further comprises a push-pull mechanism 21, wherein the push-pull mechanism 21 is used for withdrawing the magnetic member 12(a) located at the detection station J from the mounting hole when the sample container b rotates to the detection station J along with the rotary disc 11 so as to facilitate the detection device 40 to suck the magnetic compound in the sample container b; alternatively, the magnetic member 12(a) is pushed into the mounting hole before the sample container b is rotated with the turntable 11 to the pipetting station X to adsorb the magnetic composite.
Alternatively, the push-pull mechanism 21 includes a fixed substrate 211 fixed to the base 10, a slider 212 slidably disposed on the fixed substrate 211, a push plate 213 fixed to the slider 212, and a power mechanism 214 for driving the slider 212 to slide relative to the fixed substrate 211, where the push plate 213 is connected to the magnetic member 12(a), and the power mechanism 214 drives the push plate 213 to move when the slider 212 slides relative to the fixed substrate 211, so as to push the magnetic member 12(a) into the mounting hole on the base 10 or pull the magnetic member 12(a) out of the mounting hole on the base 10.
Optionally, the power mechanism 214 includes a motor 214a fixed on the fixed base plate 211, a driving wheel 214b disposed on a rotation shaft of the motor 214a, a driven wheel 214c rotatably disposed on the fixed base plate 211, and a transmission belt 214d sleeved on the driving wheel 214b and the driven wheel 214c, wherein the transmission belt 214d is fixed to the slider 212 at one position along the length direction. The drive belt 214d may be a timing belt.
Alternatively, the pushing plate 213 includes a first connecting plate 213a and a second connecting plate 213b connected to the first connecting plate 213a in a bent manner, the first connecting plate 213a is fixed to the slider 212, and the second connecting plate 213b is connected to the magnetic member 12 (a).
Optionally, the push-pull mechanism 21 further includes a slide rail 215, the slide rail 215 is disposed on the fixed substrate 211, and the slider 212 is slidably disposed on the fixed substrate 211 through the slide rail 215.
It should be understood that, in other embodiments, the push-pull mechanism may have other structures as long as the magnetic member 12(a) can be pulled out from the mounting hole and the magnetic member 12(a) can be pushed into the mounting hole.
Alternatively, one of the two ends of the magnetic member 12 in the direction of the rotation axis of the turntable 11 is an S pole, and the other is an N pole. Through the above manner, the magnetic compound Q in the sample container b can be adsorbed on two lines at two positions on the inner wall of the sample container b corresponding to the two ends of the magnetic member 12 along the direction of the rotation axis of the turntable 11. For example, as shown in the figure, the magnetic composite Q is adsorbed on the inner wall of the sample container b and corresponds to two lines at the upper and lower ends of the magnetic member 12.
In other embodiments, the magnetic member 12 may have magnetism only at both end portions in the direction of the rotation axis of the turntable 11, and the middle portion between the both end portions may not have magnetism, and of the both end portions, one of a side close to the accommodation hole a corresponding to the magnetic member 12 and a side far from the accommodation hole a corresponding to the magnetic member 12 is an S pole, and the other is an N pole, so that the magnetic compound Q is adsorbed on the inner wall of the sample container b in a concentrated manner and corresponds to the positions of the both end portions.
Referring to fig. 8, fig. 8 is a schematic top view of a magnetic separation device according to a second magnetic element layout of the present application.
In the embodiment, the magnetic member 22 is disposed at one side of the corresponding receiving hole a and is fixedly embedded on the rotating disc 11.
Each of the receiving holes a is adapted to receive a corresponding one of the sample containers b containing a sample and/or a magnetic composite, so that the magnetic composite in the sample container b adheres to the inner wall of the sample container b by the attraction of the magnetic member 22.
Since the magnetic composite in each sample container b will be attracted to the magnetic member 22 disposed in the sample container b in the attraction direction, and the relative positions of the magnetic members 22 with respect to the corresponding sample containers b are the same, the magnetic composite in the sample container b is attracted by the adjacent two magnetic members 22 in the attraction direction of the magnetic members 22 disposed in the sample containers b, so that the attraction effect of the magnetic composite can be improved.
Since the magnetic member 22 is fixed to the rotation disk 11, the magnetic member 22 rotates with the rotation disk 11, and when the rotation disk 11 rotates, the relative positions of the magnetic member 22 to the accommodating hole a and the sample container b do not change, and the direction of the attraction force of the magnetic member 22 to the magnetic compound does not change. The adsorption can be carried out while the rotary table 11 rotates, and the working efficiency and the adsorption efficiency of the magnetic separation can be improved.
The surface of the magnetic member 22 close to the receiving hole a is a plane, the surface of the receiving hole a is a circular hole, and the surface of the magnetic member 22 close to the receiving hole a is perpendicular to a reference plane determined by the rotation axis of the turntable 11 and the axis of the receiving hole a.
Optionally, the length of the magnetic member 22 is equal to the depth of the receiving hole a, so that the inner wall of the portion of the sample container b located in the receiving hole a can adsorb magnetic compounds at different positions in the height direction, thereby improving the adsorption efficiency. For example, the upper end of the magnetic member 22 may be flush with the upper end of the receiving hole a, and the lower end of the magnetic member 22 may be flush with the bottom of the receiving hole a.
Alternatively, the magnetic member 22 is shaped as a rectangular parallelepiped, i.e., each surface of the magnetic member 22 is a plane. In other embodiments, only the surface of the magnetic member 22 near the accommodating hole a may be flat.
Because the shape of the magnetic part 22 is a cuboid, and the shape of the used magnetic part 22 is a plate type, the processing difficulty is small, the cost can be effectively reduced, and the required effect can be achieved.
Alternatively, the surface of the magnetic member 22 close to the receiving hole a is spaced from the receiving hole a without direct contact, and the magnetic member 22 is embedded in the rotating disk 11. Since the magnetic member 22 is placed at a place not in contact with the sample container b, the magnetic member 22 is prevented from being corroded by the liquid that may be scattered during pipetting, and the magnetic loss of the magnetic member 22 is reduced.
In the present embodiment, the magnetic member 22 is disposed at a side of the corresponding accommodation hole a close to the rotation axis of the turntable 11. In this way, the receiving hole a can be designed closer to the edge of the turntable 11, and more receiving holes a can be arranged under the condition that the size of the turntable 11 is fixed.
In other embodiments, the magnetic member 22 may be disposed at a side of the corresponding accommodating hole a away from the rotation axis of the turntable 11, which is not limited in the embodiments of the present application.
In this embodiment, the arrangement of the withdrawing mechanism is similar to that of the above-mentioned embodiment, and in particular, refer to the structure of the withdrawing mechanism in fig. 7.
Alternatively, one of the two ends of the magnetic member 22 in the direction of the rotation axis of the turntable 11 is an S pole, and the other is an N pole. In this way, the magnetic composite in the sample container b can be adsorbed on a line (a bus closest to the magnetic member 22 when the sidewall of the sample container b is a cylindrical surface) closest to the inner wall of the magnetic member 22, and further, the magnetic composite is most densely distributed at the upper and lower ends of the sample container b. Referring specifically to fig. 6, the principle is similar.
Referring to fig. 9, fig. 9 is a schematic top view of a magnetic separation device according to a third magnetic element layout manner of the present application.
In the present embodiment, the magnetic member 52 has a ring shape, and the magnetic member 52 is disposed in the accommodating hole a and around the sample container b.
Through the mode, the magnetic piece 52 is arranged to surround the sample container b, so that the magnetic compound can be adsorbed everywhere on the inner side wall of the sample container b, and the adsorption effect of the magnetic compound is improved. Further, the magnetic part 12 with the circular ring-shaped cross section is arranged to surround the sample container c, so that the magnetic compounds adsorbed on the inner side wall of the sample container c are uniformly distributed, each magnetic compound can be subjected to uniform magnetic force, and the loss of the magnetic compounds caused in the liquid suction process is reduced.
Please refer to fig. 10 with reference to fig. 9, fig. 10 is a schematic view illustrating a magnetic attraction principle in a third magnetic element layout manner of the present application.
Alternatively, one of the two ends of the magnetic member 52 in the direction of the rotation axis of the turntable 11 is an S pole, and the other end is an N pole. In this way, the magnetic composite can be concentrated on two lines (shown as two dotted lines) on the inner wall of the sample container b corresponding to the positions of both ends of the magnetic member 52 in the direction of the rotation axis of the turntable 11 (e.g., the upper and lower ends of the magnetic member as shown in the figure).
In this embodiment, the arrangement of the withdrawing mechanism is similar to that of the above embodiment, and specific reference is made to the structure of the withdrawing mechanism. See in particular the structure of the pull-off mechanism in fig. 7.
Referring to fig. 11, fig. 11 is a schematic view illustrating another magnetizing method and an adsorption principle of the magnetic member according to the embodiment of the present application.
In the present embodiment, one of the side of the magnetic member 12 close to the corresponding receiving hole a and the other side of the magnetic member 12 away from the corresponding receiving hole a is an N pole, and the other is an S pole. In this way, the magnetic composite Q is adsorbed on the inner wall of the sample container b and on one surface of the side close to the magnetic member 12. While the magnetic member 12 is shown as a block magnet, it will be understood that this manner of magnetization is equally applicable to a ring magnet, in which case the side near the receiving hole and the side away from the receiving hole are the side on which the inner wall and the outer wall of the ring magnet are located, respectively, or inner and outer sides.
The sample analyzer of the present embodiment includes the magnetic separation device described in the above-described embodiment.
The flow-type fluoroimmunoassay analyzer of an embodiment of the present application includes a detection device and the magnetic separation device of any one of the above embodiments, the detection device including a flow cell, the detection device being configured to draw a target analyte in a sample container of the magnetic separation device into the flow cell for optical detection.
The fluorescence immunoassay analyzer specifically comprises a sample introduction device, a reagent device, an incubation device, a magnetic separation device and a detection device. The detection device comprises a flow chamber and a plurality of laser modules.
This application embodiment includes through setting up magnetic separation device: a base provided with an accommodating groove; the rotating disc is rotatably arranged in the accommodating groove, and at least one accommodating hole is formed in the rotating disc and is used for accommodating a sample container filled with a sample and/or a magnetic compound; the magnetic pieces correspond to the accommodating holes in quantity one to one, and the magnetic pieces are detachably arranged on the base or the turntable and are used for adsorbing the magnetic compound in the sample container on the inner wall of the sample container; the push-pull mechanism is arranged on the base or the turntable and used for drawing the magnetic part corresponding to the accommodating hole where the sample container is placed away from the base or the turntable when the sample container rotates to the detection station on the base along with the turntable so as to facilitate the detection device to suck the magnetic compound in the sample container; meanwhile, the quantity of the magnetic compound sucked away by the liquid sucking needle can be reduced, the adsorption effect of the magnetic compound can be improved, and the design of a detection station can be facilitated.
The above embodiments are merely examples and are not intended to limit the scope of the present disclosure, and all modifications, equivalents, and flow charts using the contents of the specification and drawings of the present disclosure or those directly or indirectly applied to other related technical fields are intended to be included in the scope of the present disclosure.

Claims (14)

1. A magnetic separation device, characterized in that it comprises:
a base provided with an accommodating groove;
the rotating disc is rotatably arranged in the accommodating groove, and at least one accommodating hole is formed in the rotating disc and is used for accommodating a sample container filled with a sample and/or a magnetic compound;
the magnetic pieces correspond to the accommodating holes in number one to one, and the magnetic pieces are detachably arranged on the base or the turntable and used for adsorbing the magnetic compound in the sample container on the inner wall of the sample container;
the push-pull mechanism is arranged on the base or the rotary table and used for drawing the magnetic part corresponding to the accommodating hole where the sample container is placed away from the base or the rotary table when the sample container rotates to the detection station on the base along with the rotary table, so that the detection device can suck the magnetic compound in the sample container.
2. A magnetic separating device according to claim 1 wherein one of the two ends of the magnetic member in the direction of the axis of rotation of the turntable is an N-pole and the other is an S-pole;
or one of the side of the magnetic part close to the accommodating hole and the other side of the magnetic part far from the accommodating hole corresponding to the magnetic part is an N pole, and the other side of the magnetic part is an S pole.
3. A magnetic separation device according to claim 2 wherein the push-pull mechanism is further adapted to push the magnetic member into the base or the turntable to attract the magnetic complexes before the sample container is rotated with the turntable to a pipetting station on the base.
4. A magnetic separation device according to claim 2 wherein the push-pull mechanism comprises a fixed substrate fixed relative to the base or the turntable, a slider slidably disposed on the fixed substrate, a push plate fixed on the slider, and a power mechanism for driving the slider to slide relative to the fixed substrate, wherein the push plate is connected to the magnetic member, and the power mechanism drives the push plate to move when driving the slider to slide relative to the fixed substrate, so as to push the magnetic member into the base or the turntable, or pull the magnetic member out of the base or the turntable.
5. A magnetic separation device according to claim 4 wherein the power mechanism comprises a motor fixed to the fixed base plate, a driving wheel disposed on a rotation shaft of the motor, a driven wheel rotatably disposed on the fixed base plate, and a transmission belt sleeved on the driving wheel and the driven wheel, the transmission belt being fixed to the slider.
6. A magnetic separation device according to claim 4 wherein the push plate comprises a first connecting plate and a second connecting plate in bent connection with the first connecting plate, the first connecting plate is fixed with the slider, and the second connecting plate is connected with the magnetic member.
7. A magnetic separation device according to claim 4 wherein the power mechanism further comprises a slide rail, the slide rail is disposed on the fixed base plate, and the slider is slidably disposed on the fixed base plate via the slide rail.
8. A magnetic separating device according to claim 2 wherein the magnetic member is disposed at a side of the corresponding receiving hole away from the rotation axis of the turntable.
9. A magnetic separator according to claim 8, wherein the surface of the magnetic member adjacent to the receiving hole is a flat surface, the receiving hole is a circular hole, and the surface of the magnetic member adjacent to the receiving hole is perpendicular to a reference plane defined by the rotation axis of the turntable and the axis of the receiving hole.
10. A magnetic separation device according to claim 2 wherein the magnetic member is annular and is disposed within the receiving bore and around the sample vessel.
11. A magnetic separation device according to claim 1 further comprising:
the light emitter and the light receiver are fixed on the base, and are oppositely arranged and arranged at intervals;
the shielding piece is fixed on the rotary disc, and when the shielding piece rotates to the corresponding positions of the light emitter and the light receiver along with the rotary disc, the shielding piece is partially positioned between the light emitter and the light receiver so as to shield the light emitted by the light emitter towards the light receiver;
the cleaning container is fixed on the shielding piece; or the cleaning container is fixed on the rotary disc at a position corresponding to the shielding piece and penetrates through the avoiding hole in the shielding piece.
12. A magnetic separation device according to claim 11 further comprising a pipetting assembly secured to the base and adapted to aspirate liquid from a sample container rotated with the turntable to a position at which the pipetting assembly is located;
the cleaning container is used for cleaning a liquid suction needle of the liquid suction assembly when the cleaning container rotates to the position of the liquid suction assembly along with the rotary disc.
13. A sample analyser, comprising a magnetic separation device according to any one of claims 1 to 12.
14. A flow-through fluoroimmunoassay analyzer comprising a detection device and a magnetic separation device according to any of claims 1 to 12, the detection device comprising a flow cell for drawing a target analyte in a sample container of the magnetic separation device into the flow cell for optical detection.
CN201811642288.7A 2018-12-29 2018-12-29 Magnetic separation device, sample analyzer, and flow type fluorescence immunoassay analyzer Pending CN111381054A (en)

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Application publication date: 20200707