CN218755763U - Nucleic acid extraction device - Google Patents
Nucleic acid extraction device Download PDFInfo
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- CN218755763U CN218755763U CN202223186850.4U CN202223186850U CN218755763U CN 218755763 U CN218755763 U CN 218755763U CN 202223186850 U CN202223186850 U CN 202223186850U CN 218755763 U CN218755763 U CN 218755763U
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Abstract
A nucleic acid extraction device comprising: the device comprises a shell, a nucleic acid extraction reagent storage mechanism, a nucleic acid extraction moving mechanism, a nucleic acid extraction mechanism and a consumable storage mechanism; the height of the consumable storage mechanism is basically equal to that of the nucleic acid extraction reagent storage mechanism and is greater than the lowest height of the pick-and-place port; the height of the lowest position of the nucleic acid extracting mechanism is greater than that of the lowest position of the taking and placing opening, and the height of the highest degree of the nucleic acid extracting mechanism is less than that of the highest position of the taking and placing opening. Through this device, can be convenient for deposit getting of mechanism and put nucleic acid extraction reagent to be convenient for change nucleic acid extraction reagent and deposit the mechanism. Meanwhile, the nucleic acid extraction mechanism is convenient to observe the nucleic acid extraction process, so that the experiment process can be mastered, and the operation condition of equipment can be observed.
Description
Technical Field
The application relates to the technical field of in-vitro diagnostic instruments, in particular to a nucleic acid extraction device.
Background
Nucleic acid (RNA, DNA) is the basic unit representing the genetic characteristics of a living body, nucleic acid detection is the biological detection of chordal tendineae at the molecular level, and has the obvious advantages of sensitivity, specificity, no window and the like compared with the traditional morphological detection, cytology detection, immunology detection and the like. The nucleic acid detection comprises technologies such as typing PCR Polymerase Chain Reaction (PCR), molecular hybridization, timed fluorescence quantitative PCR (Real time QPCR) and the like, and the first key of the nucleic acid detection technologies is to complete the extraction of nucleic acid of a biological sample, so that the effective and accurate extraction of nucleic acid becomes the premise of subsequent nucleic acid detection.
Usually carry out nucleic acid extraction to the sample through sample pretreatment device, among the nucleic acid extraction device, the position of drawing the kit is lower for the position of consumptive material frame and PCR grillage, is difficult for getting to put and draws the kit, also influences the field of vision simultaneously, is not convenient for observe the nucleic acid extraction process of sample.
SUMMERY OF THE UTILITY MODEL
The application aims to provide a nucleic acid extraction device, which is convenient for taking and placing a reagent storage mechanism, and facilitates observation of the nucleic acid extraction process of the sample.
The present application provides a nucleic acid extraction device, comprising:
the refrigerator door comprises a shell, a door body and a door body, wherein the shell is provided with an inner cavity and is provided with a taking and placing opening; a consumable storage position, a sample loading position and a nucleic acid extraction position are arranged at the bottom of the inner cavity, and the consumable storage position is close to the loading and unloading port relative to the sample loading position and the nucleic acid extraction position;
a nucleic acid extraction reagent storage mechanism for storing a reagent for nucleic acid extraction of a sample;
a nucleic acid extraction movement mechanism comprising: the nucleic acid extraction driving mechanism is connected with the nucleic acid extraction reagent storage mechanism through the gap and is used for driving the nucleic acid extraction reagent storage mechanism to be switched between the sample adding position and the nucleic acid extraction position in a reciprocating manner;
the nucleic acid extraction mechanism is arranged above the nucleic acid extraction position and is used for extracting nucleic acid from a sample;
the consumable storage mechanism is arranged at the consumable storage position and used for storing the transferred sample, the sample subjected to nucleic acid extraction, the PCR reaction reagent and the consumable for carrying out PCR reaction;
wherein the height of the consumable storage mechanism is basically equal to that of the nucleic acid extraction reagent storage mechanism and is greater than the lowest height of the pick-and-place port; the height of the lowest position of the nucleic acid extracting mechanism is greater than that of the lowest position of the taking and placing opening, and the height of the highest position of the nucleic acid extracting mechanism is less than that of the highest position of the taking and placing opening.
In one embodiment, the outer side of the mounting plate facing away from the opening is further provided with a blocking assembly for blocking liquid.
In one embodiment, the barrier assembly is an annular barrier groove disposed around the nucleic acid extraction drive mechanism.
In one embodiment, the consumable storage mechanism comprises: the kit comprises a sucker storing assembly, a PCR reaction assembly and a PCR reagent storing assembly, wherein the sucker storing assembly is used for storing suckers, the PCR reaction assembly is provided with PCR reaction holes, and the PCR reagent storing assembly is used for storing PCR reaction reagents; the heights of the suction head storage assembly, the PCR reaction assembly and the PCR reagent storage assembly are basically the same.
In one embodiment, a tip storage assembly comprises: the suction head comprises a first suction head frame, a second suction head frame and a third suction head frame, wherein the first suction head frame is provided with a plurality of rows of first storage rows, the first storage rows are provided with a plurality of first storage grooves, and the first storage grooves are used for storing the first suction heads; the second sucker frame is provided with a plurality of rows of second storage rows, the second storage rows are provided with a plurality of second storage grooves, and the second storage grooves are used for storing the second suckers; the third sucker frame is provided with a plurality of rows of third storage rows, each third storage row is provided with a plurality of third storage grooves, and the third storage grooves are used for storing the third suckers; the first suction head is used for transferring a sample, the second suction head is used for transferring the sample after nucleic acid extraction is completed, and the third suction head is used for transferring PCR reaction reagents to the PCR reaction assembly.
In one embodiment, the nucleic acid extraction drive mechanism comprises: the reagent storage device comprises a driving source, a connecting piece and a belt transmission component, wherein the connecting piece is provided with a first connecting side and a second connecting side, the first connecting side is connected with the nucleic acid extraction reagent storage mechanism, the second connecting side extends to the outer side of the mounting plate through the gap and is connected with the belt transmission component, the belt transmission component can output reciprocating motion from a sample adding position to the nucleic acid extraction position, and the driving source is connected with the belt transmission component and is used for providing power for the belt transmission component.
In one embodiment, the nucleic acid extraction driving mechanism further comprises: the second connecting side of the sliding guide assembly is further connected with the sliding guide assembly, and the sliding guide assembly can output movement in the same direction as the belt transmission assembly and is used for guiding the movement of the connecting piece.
In one embodiment, the nucleic acid extraction driving mechanism further comprises: first response module and second response module, the lateral surface of mounting panel has first installation position and second installation position, first response module set up in first installation position, second response module set up in second installation position, first response module with second response module all with the driving source electricity is connected, first response module is used for the driving source drive belt drive subassembly drives nucleic acid extraction reagent deposits the mechanism and removes to when the sample application of sample position, control the driving source stop work, second response module is used for the driving source drive belt drive subassembly drives nucleic acid extraction reagent deposits the mechanism and removes to when the nucleic acid extraction position, control the driving source stop work.
In one embodiment, the nucleic acid extraction reagent storage mechanism comprises: a plurality of reagent case that set up side by side, be equipped with the mutual parallel arrangement's of multiunit in the reagent case and deposit and arrange the subassembly, it includes to deposit and arrange the subassembly: the device comprises a cracking liquid storage row, a washing liquid storage row and an eluent storage row, wherein the cracking liquid storage row is provided with a plurality of cracking liquid storage holes, the washing liquid storage row is provided with a plurality of washing liquid storage holes, and the eluent storage row is provided with a plurality of eluent storage holes.
In one embodiment, the nucleic acid extraction mechanism comprises: the device comprises a support, a magnetic rod frame lifting driving assembly, a magnetic rod sleeve frame lifting driving assembly and a translation driving assembly, wherein the support is installed above a nucleic acid extraction position; the magnetic rod rack is provided with a plurality of magnetic rods, the number and the interval of the magnetic rods are the same as those of a single row of hole sites in the reagent storage box, the magnetic rod sleeve rack is provided with a plurality of magnetic rod sleeves, the number and the interval of the magnetic rod sleeves are the same as those of a single row of hole sites in the reagent storage box, each magnetic rod sleeve corresponds to one magnetic rod, the magnetic rod rack lifting driving assembly is used for driving the magnetic rods to descend so as to extend into the magnetic rod sleeves and drive the magnetic rods to ascend so as to move out of the hole sites, and the magnetic rod sleeve rack lifting driving assembly is used for driving the magnetic rod sleeves to descend so as to extend into the hole sites and drive the magnetic rod sleeves to ascend so as to move out of the hole sites; the translation driving component is used for driving the magnetic rod rack lifting driving component and the magnetic rod sleeve rack lifting driving component to synchronously move so as to enable the magnetic rod sleeve to be respectively switched to the position above the lysate storage hole, the position above the washing liquid storage hole and the position above the eluent storage hole; the lowest position of the magnetic rod sleeve is the lowest position of the nucleic acid extraction mechanism, and the highest point of the magnetic rod rack lifting driving assembly or the magnetic rod sleeve rack lifting driving assembly is the highest point of the nucleic acid extraction mechanism.
According to the nucleic acid extraction device of the embodiment, the height of the consumable storage mechanism is set to be substantially equal to that of the nucleic acid extraction reagent storage mechanism, and the height of the consumable storage mechanism and that of the nucleic acid extraction reagent storage mechanism are only larger than the lowest height of the taking and placing port, so that the nucleic acid extraction reagent storage mechanism can be conveniently taken and placed, and the nucleic acid extraction reagent storage mechanism can be conveniently replaced. The nucleic acid extraction mechanism is positioned above the nucleic acid extraction position, the height of the lowest position of the nucleic acid extraction mechanism is greater than the height of the lowest position of the taking and placing port, and the height of the highest position of the nucleic acid extraction mechanism is less than the height of the highest position of the taking and placing port, so that the process of nucleic acid extraction of the nucleic acid extraction mechanism can be observed through the taking and placing port, the experiment process can be conveniently mastered, and the operation condition of the equipment can be observed.
Drawings
FIG. 1 is a perspective view of a nucleic acid extraction device provided herein;
FIG. 2 is a top view of a nucleic acid isolation apparatus removal housing provided in the present application;
FIG. 3 is a perspective view of the nucleic acid extraction reagent storage mechanism of the nucleic acid extraction apparatus according to the present invention after being engaged with the nucleic acid extraction moving mechanism;
FIG. 4 is a bottom view of the nucleic acid extracting reagent storage mechanism of the nucleic acid extracting apparatus according to the present application after cooperating with the nucleic acid extracting moving mechanism;
FIG. 5 is a perspective view of a consumable storage mechanism in the nucleic acid isolation apparatus provided in the present application;
FIG. 6 is a first perspective view of a nucleic acid extraction mechanism in the nucleic acid extraction apparatus provided herein;
FIG. 7 is a second perspective view of the nucleic acid isolation mechanism of the nucleic acid isolation apparatus of the present application.
Description of reference numerals:
a shell 10, an inner cavity 11, a taking and placing opening 12 and a door body 13
A nucleic acid extraction reagent storage mechanism 20, a reagent storage box 21, a lysate storage row 211, a wash solution storage row 212, an eluent storage row 213, a lysate storage hole 214, a wash solution storage hole 215, and an eluent storage hole 216;
a nucleic acid extraction moving mechanism 30, a mounting plate 31, an annular blocking groove 311, a nucleic acid extraction driving mechanism 32, a driving source 321, a connecting piece 322, a belt transmission component 323, a driving belt wheel 3231, a driven belt wheel 3232, a transmission belt 3233, a clamping plate 3234, a sliding guide component 324, a first induction module 325 and a second induction module 326;
the nucleic acid extraction mechanism 40, a bracket 41, a magnetic rod rack 42, a magnetic rod 420, a magnetic rod rack lifting driving assembly 43, a magnetic rod sleeve rack 44, a magnetic rod sleeve 440, a magnetic rod sleeve rack lifting driving assembly 45 and a translation driving assembly 46;
a consumable storage mechanism 50, a tip storage assembly 51, a first tip rack 511, a second tip rack 512, a third tip rack 513, a first tip 514, a second tip 515, a third tip 516, a PCR reaction assembly 52, a PCR reagent storage assembly 53,
a sample storage mechanism 60, a sample container 61;
a liquid transfer mechanism 70;
A consumable storage position A, a sample adding position B, a nucleic acid extraction position C and a sample storage position D.
Detailed Description
The present application will be described in further detail below with reference to the accompanying drawings by way of specific embodiments. Wherein like elements in different embodiments have been given like element numbers associated therewith. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.
Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments, and the operation steps involved in the embodiments may be interchanged or modified in order as will be apparent to those skilled in the art. Accordingly, the description and drawings are merely for clarity of description of certain embodiments and are not intended to necessarily refer to a required composition and/or order.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).
The nucleic acid extraction device provided by the application is used for extracting nucleic acid of a biological sample and then carrying out PCR (Polymerase Chain Reaction), wherein the PCR is a technology for in vitro enzymatic synthesis of specific DNA fragments and is also a commonly used experimental technology for detection. In this embodiment, the biological sample may be saliva, blood, tissue, sputum, or the like.
In the nucleic acid extracting device, the height of the nucleic acid extracting reagent storing mechanism is basically equal to the height of the consumable storing mechanism, and the height of the nucleic acid extracting reagent storing mechanism is greater than the lowest height of the taking and placing port, so that the nucleic acid extracting reagent storing mechanism is convenient to take and place. The height of the lowest position of the nucleic acid extracting mechanism is larger than the height of the lowest position of the picking and placing port, and the height of the highest position of the nucleic acid extracting mechanism is smaller than the height of the highest position of the picking and placing port, so that the nucleic acid extracting process of the sample can be observed conveniently.
Referring to FIGS. 1 and 2, the nucleic acid extraction apparatus provided in this example includes: a casing 10, a nucleic acid extracting reagent storing mechanism 20, a nucleic acid extracting moving mechanism 30, a nucleic acid extracting mechanism 40, and a consumable storing mechanism 50.
The shell 10 is provided with an inner cavity 11, the shell 10 is provided with a taking and placing opening 12, a door body 13 is arranged at the taking and placing opening 12, and the door body 13 can open or close the taking and placing opening 12. A consumable storage position A, a sample loading position B and a nucleic acid extraction position C are arranged at the bottom of the inner cavity 11, and the consumable storage position A is close to the loading and unloading port 12 relative to the sample loading position B and the nucleic acid extraction position C. As shown in FIG. 2, the positions of the consumable storage site A, the sample addition site B and the nucleic acid extraction site C are roughly outlined by dashed lines in the figure.
The nucleic acid extraction reagent storage unit 20 is for storing a reagent for extracting nucleic acid from a biological sample such as saliva, blood, tissue, sputum, or the like.
Referring to FIGS. 3 and 4, the nucleic acid extracting and moving mechanism 30 includes: mounting panel 31 and nucleic acid extraction actuating mechanism 32, the opening has been seted up to the bottom of casing 10, and mounting panel 31 sets up in the opening, and mounting panel 31 and opening have the space, and nucleic acid extraction actuating mechanism 32 sets up in mounting panel 31 dorsad the lateral surface of inner chamber, nucleic acid extraction reagent storage mechanism 20 set up in the medial surface that mounting panel 31 is located inner chamber 11, and nucleic acid extraction actuating mechanism 32 is connected through space and nucleic acid extraction reagent storage mechanism 20, and nucleic acid extraction actuating mechanism 32 is used for driving nucleic acid extraction reagent storage mechanism 20 and reciprocates between sample application of sample position B and nucleic acid extraction position C.
The nucleic acid extraction means 40 is provided above the nucleic acid extraction site C, and the nucleic acid extraction means 40 is used for extracting nucleic acid from a sample.
The consumptive material is deposited mechanism 50 and is located the consumptive material and deposit position A, and the consumptive material is deposited mechanism 50 and is used for depositing sample, the sample of having accomplished the nucleic acid extraction, PCR reactant to and deposit the consumptive material that carries out the PCR reaction.
In this embodiment, the consumable materials for transferring the sample, completing the extraction of nucleic acid, and PCR reaction reagent are usually tips, and of course, the types of the tips for transferring the sample, completing the extraction of nucleic acid, and completing the PCR reaction reagent may be different or the same, and are specifically selected according to actual needs.
As shown in fig. 1 and 2, a sample storage place D is further provided in the internal cavity 11, the position of the sample storage place D is roughly indicated by a dotted line in fig. 2, a sample storage mechanism 60 is provided in the sample storage place D, a plurality of sample containers 61 are provided in the sample storage mechanism 60, and the sample containers 61 are used for storing samples. The sample is transferred to the nucleic acid extraction reagent storage means 20 via the tip, and the nucleic acid extraction driving means 32 drives the nucleic acid extraction reagent storage means 20 to which the sample has been added from the sample addition site B to the nucleic acid extraction site C, and the nucleic acid is extracted from the sample added to the nucleic acid extraction reagent by the nucleic acid extraction means 40. After the nucleic acid extraction is completed, the nucleic acid extraction driving mechanism 32 drives the nucleic acid extraction reagent storage mechanism 20 to move from the nucleic acid extraction site C to the sample loading site B, loads the sample from which the nucleic acid extraction has been completed to the consumable material for performing the PCR reaction through the tip, and loads the PCR reaction reagent to the consumable material for the PCR reaction through another tip, and then can perform the PCR reaction construction on the sample from which the nucleic acid extraction has been completed.
In the above embodiment, the transfer of the sample, the sample from which the extraction of nucleic acid has been completed, and the PCR reaction reagent may be manually performed, but it is needless to say that the transfer may be performed by the liquid transfer mechanism 70 in order to ensure the accuracy and the efficiency. Specifically, the pipetting mechanism 70 moves to the consumable storage position a, and the pipetting pump on the pipetting mechanism 70 descends to sleeve the pipette tip on the pipetting pump, the pipetting mechanism 70 moves to the sample storage position D again to adsorb the sample to the pipette tip by the negative pressure adsorption force generated by the pipetting pump, and the pipetting mechanism 70 moves to the sample addition position B and releases the sample to the reagent of the nucleic acid extraction reagent storage mechanism 20 by releasing the negative pressure adsorption force. The pipetting mechanism 70 repeats the above steps until all the samples are added to the reagents of the nucleic acid extracting reagent storing mechanism 20. After the nucleic acid extraction of the sample is completed, the pipetting mechanism 70 adds the sample with the nucleic acid extraction completed into the PCR reaction consumable through the pipette tip, and adds the PCR reaction reagent into the PCR reaction consumable to perform the PCR reaction construction.
As described above, the tip is required to be replaced with a new one every time the transfer of the sample is completed, the transfer of the extracted nucleic acid sample is completed, and the transfer of the PCR reaction reagent is not completed, and thus, the waste tip is required to be collected, and therefore, the waste collection mechanism 80 is further provided in the inner cavity 11 to collect the waste tip. In other words, after the transfer operation is completed, the pipetting mechanism 70 is moved to above the waste collection mechanism 80 and the waste tips are ejected into the waste collection mechanism 80.
The nucleic acid extracting reagent storing means 20 is a waste product, and when one nucleic acid extraction is completed or nucleic acid extraction is performed on a different sample, it is necessary to replace the nucleic acid extracting reagent storing means 20 with a new one, and naturally, a new nucleic acid extracting reagent is stored in the new nucleic acid extracting reagent storing means 20. Further, the overall height of the conventional nucleic acid extraction reagent storage mechanism 20 is higher than that of the consumable storage mechanism 50, which is not convenient for taking and placing the nucleic acid extraction reagent storage mechanism 20 and for observing the sample addition process in the nucleic acid extraction reagent storage mechanism 20, therefore, in the present embodiment, the height of the consumable storage mechanism 50 is substantially equal to that of the nucleic acid extraction reagent storage mechanism 20, and the height of the consumable storage mechanism 50 and the height of the nucleic acid extraction reagent storage mechanism 20 are both greater than the lowest height of the sample inlet 12. Thus, the storage mechanism 20 for nucleic acid extraction reagent is convenient to take and place, and the storage mechanism for nucleic acid extraction reagent is convenient to replace.
In this embodiment, the nucleic acid extraction mechanism 40 is located above the nucleic acid extraction site C, and the height of the lowest position of the nucleic acid extraction mechanism 40 is greater than the height of the lowest position of the access port 12, and the height of the highest position of the nucleic acid extraction mechanism 40 is less than the height of the highest position of the access port 12, so that the nucleic acid extraction mechanism 40 can be observed through the access port 12, and further the process of nucleic acid extraction performed by the nucleic acid extraction mechanism 40 can be observed conveniently, so that the experimental process can be grasped, and the operation condition of the device can be observed. Of course, the whole experiment process is performed in a closed environment, that is, in a state where the door 13 is in a state of closing the access opening 12, so that the door 13 may be made transparent, or a transparent window may be provided on the door 13 or the housing 10.
In the experiment process, the risk of reagent dripping is inevitable, and in order to avoid reagent corrosion to the nucleic acid extraction driving mechanism 32, the nucleic acid extraction driving mechanism 32 is arranged on the outer side surface of the mounting plate 31, which is opposite to the inner cavity 11. It is considered that the surface of the mounting plate 31 located inside the cavity 11 is the inner surface of the mounting plate 11, and the outer surface of the mounting plate 31 facing away from the cavity 11 is the outer surface of the mounting plate, so that the nucleic acid extraction driving mechanism 32 is prevented from being corroded by the reagent.
In one embodiment of the present application, a blocking component is further disposed on the outer side of the mounting plate 31 opposite to the opening opened at the bottom of the housing 10, and the blocking component is used for blocking liquid, i.e., dripping reagent, so as to further prevent the reagent from flowing to the nucleic acid extraction driving mechanism 32 along the edge of the mounting plate 31.
In this embodiment, as shown in FIG. 4, the blocking member is an annular blocking groove 311, and the annular blocking groove 311 is disposed around the nucleic acid extraction driving mechanism 32.
With continued reference to FIG. 4, the nucleic acid extraction drive mechanism 32 includes: a driving source 321, a connecting member 322, and a belt transmission member 323, wherein the connecting member 322 has a first connecting side and a second connecting side, the first connecting side is connected to the nucleic acid extracting reagent storing mechanism 20, the second connecting side extends to the outer side of the mounting plate 31 through the gap between the mounting plate 31 and the opening and is connected to the belt transmission member 323, the belt transmission member 323 can output reciprocating movement from the sample loading position B to the nucleic acid extracting position C, the driving source 321 preferably adopts a motor, the driving source 321 is connected to the belt transmission member 323, and the driving source 321 is used for providing power for the belt transmission member 323, so that the belt transmission member 323 drives the nucleic acid extracting reagent storing mechanism 20 to reciprocate between the sample loading position B and the nucleic acid extracting position C through the connecting member 322, so that the nucleic acid extracting reagent storing mechanism 20 can be switched to the sample loading position B or the nucleic acid extracting position C.
In this embodiment, the belt drive assembly 323 includes: a driving pulley 3231, a driven pulley 3232 and a transmission belt 3233, wherein the driving pulley 3231 is installed at a power output end of the driving source 321, the driving pulley 3231 and the driven pulley 3232 are installed along a direction from the sample loading position B to the nucleic acid extracting position C, the driven pulley 3232 is rotatably installed on an outer side surface of the installation plate 31, the transmission belt 3233 is sleeved on the driving pulley 3231 and the driven pulley 3232, a second connection side of the connection member 322 is fixedly connected with a side belt surface of the transmission belt 3233 through a clamping plate 3234, the driving source 321 can drive the driving pulley 3231 to rotate around its own axis, the rotating driving pulley 3231 drives the driven pulley 3232 to rotate through the transmission belt 3233, the 3233 can convert the rotation motion of the driving pulley 3231 and the driven pulley 3233 into a linear motion along a length direction of the transmission belt 3232, and the driving source 321 can output a reciprocating rotation motion through program setting, so that the transmission belt can drive the nucleic acid extracting reagent storage mechanism 20 to move between the sample loading position B and the nucleic acid extracting position C through the clamping plate 3234.
In one embodiment of the present application, the connecting line between the sample storage site B and the nucleic acid extraction site C is a straight line, which is the straight line along the length direction of the transmission belt 3233, so that the nucleic acid extraction reagent storage mechanism 20 can be switched between the sample loading site B and the nucleic acid extraction site C.
The nucleic acid extraction driving mechanism 32 further includes: the sliding guide assembly 324 is also connected to the second connecting side of the sliding guide assembly 324, and the sliding guide assembly 324 can output movement in the same direction as the belt transmission assembly 323, so as to guide the movement of the connecting member 322, and ensure the stability of the movement of the connecting member 322.
In one embodiment of the present application, the sliding guide assembly 324 may be comprised of a guide rail having a length direction parallel to a length direction of the driving belt 3233 and a slider slidably disposed on the guide rail and coupled to the second coupling side of the coupling member 322.
With continued reference to FIG. 4, the nucleic acid extraction drive mechanism 32 further includes: first response module 325 and second response module 326, the lateral surface of mounting panel 31 has first installation position and second installation position, first response module 325 sets up in first installation position, second response module 326 sets up in the second installation position, first response module 325 and second response module 326 all are connected with driving source 321 electricity, first response module 325 is used for driving belt drive subassembly 323 at driving source 321 and drives nucleic acid extraction reagent storage mechanism 20 and remove to sample application of sample position B, control driving source 321 stop work to remove nucleic acid extraction reagent storage mechanism 20 to sample application of sample position B. The second sensing module 326 is used for controlling the driving source 321 to stop working when the driving source 321 drives the belt transmission assembly 323 to drive the nucleic acid extracting reagent storing mechanism 20 to move to the nucleic acid extracting site C, so as to move the nucleic acid extracting reagent storing mechanism 20 to the nucleic acid extracting site C.
In this embodiment, the first sensing module 325 and the second sensing module 326 may both adopt proximity switches.
Referring to fig. 5, the consumable storing mechanism 50 includes: a tip storage assembly 51, a PCR reaction assembly 52 and a PCR reagent storage assembly 53, wherein the tip storage assembly 51 is used for storing the tip, the PCR reaction assembly 52 is provided with a PCR reaction hole 520, and the PCR reagent storage assembly 53 is used for storing the PCR reaction reagent. In this embodiment, the heights of the tip storage module 51, the PCR reaction module 52, and the PCR reagent storage module 53 are substantially the same.
With continued reference to fig. 5, the tip storage assembly 51 comprises: the first suction head rack 511, the second suction head rack 512 and the third suction head rack 513 comprise a first suction head 514, a second suction head 515 and a third suction head 516, the first suction head rack 511 is provided with a plurality of rows of first storage rows 5110, and the first storage rows 5110 are provided with a plurality of first storage grooves for storing the first suction head 514. The second tip rack 512 is provided with a plurality of rows of second rows 5120, and the second rows 5120 is provided with a plurality of second storage slots for storing the second tips 515. The third head frame 513 is provided with a plurality of rows of third storage rows 5130, the third row 5130 is provided with a plurality of third storage troughs for storing the third suckers 516. The first tip 514 is used to transfer the sample, the second tip 515 is used to transfer the sample from which the nucleic acid extraction has been completed, and the third tip 516 is used to transfer the PCR reaction reagent to the PCR reaction assembly 52.
In one embodiment of the application, the first tip 514 is a 1000. Mu.L tip, the second tip 515 is a 50. Mu.L tip, and the third tip 516 is a 200. Mu.L tip. Of course, the capacity of the first suction head 514, the second suction head 515 and the third suction head 516 can be selected according to actual needs.
Meanwhile, since the volumes of the first suction head 514, the second suction head 515, and the third suction head 516 are different, the interval between two adjacent first storage tanks in the first row 5110 of the same row, the interval between two adjacent second storage tanks in the second row 5120 of the same row, and the interval between two adjacent third storage tanks in the third row 5130 of the same row are different from each other.
As shown in FIG. 3, the nucleic acid extracting reagent storing mechanism 20 includes: a plurality of reagent case 21, each reagent case 21 sets up side by side, all is equipped with the row's subassembly of depositing of multiunit mutual parallel arrangement in every reagent case 21, should deposit row's subassembly and include: a lysate storage row 211, a washing solution storage row 212, and an eluent storage row 213, the lysate storage row 211 is provided with a plurality of lysate storage holes 214, the lysate storage holes 214 store the lysate, the washing liquid storage and discharge section 212 is provided with a plurality of washing liquid storage holes 215, the washing liquid storage holes 215 store washing liquid, the washing liquid storage and discharge section 213 is provided with a plurality of washing liquid storage holes 216, and the washing liquid storage holes 216 store washing liquid.
In this embodiment, the number and the positions of the lysis buffer storage holes 214 provided in the lysis buffer storage row 211, the number and the positions of the washing solution storage holes 215 provided in the washing solution storage row 212, and the number and the positions of the eluent storage holes 216 provided in the eluent storage row 213 are all the same.
Referring to FIGS. 6 and 7, the nucleic acid extracting mechanism 40 includes: support 41, bar magnet frame 42, bar magnet frame lift drive assembly 43, bar magnet cover frame 44, bar magnet cover frame lift drive assembly 45 and translation drive assembly 46, support 41 is installed in the top of nucleic acid extraction position C, translation drive assembly 46 is installed in support 41, bar magnet frame lift drive assembly 43 and bar magnet cover frame lift drive assembly 45 are all installed in translation drive assembly 46, bar magnet frame 42 locates the power take off end of bar magnet frame lift drive assembly 43, bar magnet cover frame 44 locates the power take off end of bar magnet cover frame lift drive assembly 45, and bar magnet frame 42 is located the top of bar magnet cover frame 44. The plurality of magnetic rods 420 with the same number and the same spacing as the single-row holes in the reagent storage box 21 are arranged on the magnetic rod rack 42, and the plurality of magnetic rod sleeves 440 with the same number and the same spacing as the single-row holes in the reagent storage box 21 are arranged on the magnetic rod sleeve rack 44, wherein the single-row holes in the reagent storage box 21 are the cracking liquid storage hole 214 arranged in the cracking liquid storage row 211, the washing liquid storage hole 215 arranged in the washing liquid storage row 212, and the washing liquid storage hole 216 arranged in the washing liquid storage row 213, and each magnetic rod sleeve 440 corresponds to one magnetic rod 420. The magnetic rod rack lifting driving assembly 43 is used for driving the magnetic rod 420 to descend so as to extend into the magnetic rod sleeve 440 and driving the magnetic rod 420 to ascend so as to move out of the magnetic rod sleeve 440, and the magnetic rod sleeve rack lifting driving assembly 45 is used for driving the magnetic rod sleeve 440 to descend so as to extend into the hole site and driving the magnetic rod sleeve 440 to ascend so as to move out of the hole site. The translation driving assembly 46 is used for driving the magnetic rod rack lifting driving member 43 and the magnetic rod sleeve rack lifting driving assembly 45 to move synchronously, so that the magnetic rod sleeve 440 is switched to the upper part of the lysis solution storage hole 214, the upper part of the washing solution storage hole 215 and the upper part of the eluent storage hole 216 respectively. In this embodiment, the lowest point of the magnetic rod sleeve 440 is the lowest point of the nucleic acid extracting mechanism 40, and the highest point of the magnetic rod rack lifting driving assembly 43 or the magnetic rod sleeve rack lifting driving assembly 45 is the highest point of the nucleic acid extracting mechanism 40.
In summary, in the nucleic acid extracting apparatus provided by this embodiment, the height of the consumable storage mechanism is set to be substantially equal to the height of the nucleic acid extracting reagent storage mechanism, and the height of the consumable storage mechanism and the height of the nucleic acid extracting reagent storage mechanism are only greater than the lowest height of the pick-and-place opening, so that the nucleic acid extracting reagent storage mechanism can be conveniently picked and placed, and the nucleic acid extracting reagent storage mechanism can be conveniently replaced. The nucleic acid extraction mechanism is positioned above the nucleic acid extraction position, the height of the lowest position of the nucleic acid extraction mechanism is greater than the height of the lowest position of the taking and placing port, and the height of the highest position of the nucleic acid extraction mechanism is less than the height of the highest position of the taking and placing port, so that the process of nucleic acid extraction of the nucleic acid extraction mechanism can be observed through the taking and placing port, the experimental process can be conveniently mastered, and the running condition of equipment can be observed.
It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.
Claims (10)
1. A nucleic acid extraction device, comprising:
the refrigerator door comprises a shell, a door body and a door body, wherein the shell is provided with an inner cavity and is provided with a taking and placing opening; a consumable storage position, a sample loading position and a nucleic acid extraction position are arranged at the bottom of the inner cavity, and the consumable storage position is close to the loading and unloading port relative to the sample loading position and the nucleic acid extraction position;
a nucleic acid extraction reagent storage mechanism for storing a reagent for performing nucleic acid extraction on a sample;
a nucleic acid extraction movement mechanism comprising: the nucleic acid extraction driving mechanism is connected with the nucleic acid extraction reagent storage mechanism through the gap and is used for driving the nucleic acid extraction reagent storage mechanism to be switched between the sample adding position and the nucleic acid extraction position in a reciprocating manner;
the nucleic acid extraction mechanism is arranged above the nucleic acid extraction position and is used for extracting nucleic acid from a sample;
the consumable storage mechanism is arranged at the consumable storage position and used for storing the transferred sample, the sample subjected to nucleic acid extraction, the PCR reaction reagent and the consumable for carrying out PCR reaction;
wherein the height of the consumable storage mechanism is basically equal to that of the nucleic acid extraction reagent storage mechanism and is greater than the lowest height of the pick-and-place port; the height of the lowest position of the nucleic acid extracting mechanism is greater than that of the lowest position of the taking and placing opening, and the height of the highest position of the nucleic acid extracting mechanism is less than that of the highest position of the taking and placing opening.
2. The nucleic acid extraction apparatus according to claim 1, wherein an outer side of the mounting plate facing away from the opening is further provided with a blocking member for blocking liquid.
3. The nucleic acid extraction device according to claim 2, wherein the blocking member is an annular blocking groove provided around the nucleic acid extraction drive mechanism.
4. The nucleic acid extraction apparatus according to claim 1, wherein the consumable storage mechanism includes: the kit comprises a sucker storing assembly, a PCR reaction assembly and a PCR reagent storing assembly, wherein the sucker storing assembly is used for storing suckers, the PCR reaction assembly is provided with PCR reaction holes, and the PCR reagent storing assembly is used for storing PCR reaction reagents; the heights of the suction head storage assembly, the PCR reaction assembly and the PCR reagent storage assembly are basically the same.
5. The nucleic acid extraction device of claim 4, wherein the tip storage assembly comprises: the suction head comprises a first suction head frame, a second suction head frame and a third suction head frame, wherein the first suction head frame is provided with a plurality of rows of first storage rows, the first storage rows are provided with a plurality of first storage grooves, and the first storage grooves are used for storing the first suction heads; the second sucker frame is provided with a plurality of rows of second storage rows, the second storage rows are provided with a plurality of second storage grooves, and the second storage grooves are used for storing the second suckers; the third sucker frame is provided with a plurality of rows of third storage rows, each third storage row is provided with a plurality of third storage grooves, and the third storage grooves are used for storing the third suckers; the first sucker is used for transferring a sample, the second sucker is used for transferring the sample after nucleic acid extraction is completed, and the third sucker is used for transferring PCR reaction reagents to the PCR reaction assembly.
6. The nucleic acid extraction apparatus according to claim 1, wherein the nucleic acid extraction driving mechanism comprises: the reagent storage device comprises a driving source, a connecting piece and a belt transmission component, wherein the connecting piece is provided with a first connecting side and a second connecting side, the first connecting side is connected with the nucleic acid extraction reagent storage mechanism, the second connecting side extends to the outer side of the mounting plate through the gap and is connected with the belt transmission component, the belt transmission component can output reciprocating motion from a sample adding position to the nucleic acid extraction position, and the driving source is connected with the belt transmission component and is used for providing power for the belt transmission component.
7. The nucleic acid extraction apparatus according to claim 6, wherein the nucleic acid extraction driving mechanism further comprises: the second connecting side of the sliding guide assembly is further connected with the sliding guide assembly, and the sliding guide assembly can output movement in the same direction as the belt transmission assembly and is used for guiding the movement of the connecting piece.
8. The nucleic acid extraction apparatus according to claim 7, wherein the nucleic acid extraction driving mechanism further comprises: first response module and second response module, the lateral surface of mounting panel has first installation position and second installation position, first response module set up in first installation position, second response module set up in second installation position, first response module with second response module all with the driving source electricity is connected, first response module is used for the driving source drive belt drive subassembly drives nucleic acid draws reagent and deposits the mechanism and remove to when the sample application of sample position, control the driving source stop work, second response module is used for the driving source drive belt drive subassembly drives nucleic acid draws reagent and deposits the mechanism and remove to when the nucleic acid draws the position, control the driving source stop work.
9. The nucleic acid extraction apparatus according to claim 1, wherein the nucleic acid extraction reagent storage mechanism comprises: a plurality of reagent case that set up side by side, be equipped with the mutual parallel arrangement's of multiunit in the reagent case and deposit and arrange the subassembly, it includes to deposit and arrange the subassembly: the device comprises a cracking liquid storage row, a washing liquid storage row and an eluent storage row, wherein the cracking liquid storage row is provided with a plurality of cracking liquid storage holes, the washing liquid storage row is provided with a plurality of washing liquid storage holes, and the eluent storage row is provided with a plurality of eluent storage holes.
10. The nucleic acid extraction apparatus according to claim 9, wherein the nucleic acid extraction mechanism comprises: the device comprises a support, a magnetic rod frame lifting driving assembly, a magnetic rod sleeve frame lifting driving assembly and a translation driving assembly, wherein the support is installed above a nucleic acid extraction position; the magnetic rod rack is provided with a plurality of magnetic rods, the number and the interval of the magnetic rods are the same as those of a single row of hole sites in the reagent storage box, the magnetic rod sleeve rack is provided with a plurality of magnetic rod sleeves, the number and the interval of the magnetic rod sleeves are the same as those of a single row of hole sites in the reagent storage box, each magnetic rod sleeve corresponds to one magnetic rod, the magnetic rod rack lifting driving assembly is used for driving the magnetic rods to descend so as to extend into the magnetic rod sleeves and drive the magnetic rods to ascend so as to move out of the hole sites, and the magnetic rod sleeve rack lifting driving assembly is used for driving the magnetic rod sleeves to descend so as to extend into the hole sites and drive the magnetic rod sleeves to ascend so as to move out of the hole sites; the translation driving component is used for driving the magnetic rod rack lifting driving component and the magnetic rod sleeve rack lifting driving component to synchronously move so as to enable the magnetic rod sleeve to be respectively switched to the position above the lysate storage hole, the position above the washing liquid storage hole and the position above the eluent storage hole; the lowest position of the magnetic rod sleeve is the lowest position of the nucleic acid extraction mechanism, and the highest point of the magnetic rod rack lifting driving component or the magnetic rod sleeve rack lifting driving component is the highest point of the nucleic acid extraction mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223186850.4U CN218755763U (en) | 2022-11-29 | 2022-11-29 | Nucleic acid extraction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223186850.4U CN218755763U (en) | 2022-11-29 | 2022-11-29 | Nucleic acid extraction device |
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| CN218755763U true CN218755763U (en) | 2023-03-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202223186850.4U Active CN218755763U (en) | 2022-11-29 | 2022-11-29 | Nucleic acid extraction device |
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| Country | Link |
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| CN (1) | CN218755763U (en) |
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