CN106706379A - Device and method for treating slide specimens - Google Patents
Device and method for treating slide specimens Download PDFInfo
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- CN106706379A CN106706379A CN201610798084.7A CN201610798084A CN106706379A CN 106706379 A CN106706379 A CN 106706379A CN 201610798084 A CN201610798084 A CN 201610798084A CN 106706379 A CN106706379 A CN 106706379A
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- slide glass
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Abstract
The invention discloses a device and a method for treating slide specimens. The device mainly comprises a container, a heating device, a liquid drain outlet, a liquid inlet, a controller, a liquid drain micropump, a liquid inlet micropump and a thermocouple. According to the device, a large number of slide specimens can be subjected to reagent sample adding, cleaning, heat treatment, thermal insulation, dehydration and other common slide specimen detection steps in the same device, and especially, for multi-step operation of immunohistochemistry, in-situ hybridization as well as protein detection and DNA nucleotide and gene detection of other slide specimens, all slide specimens are treated in one device from beginning to end; slides are not required to be manually taken or transferred in the whole process of the slide specimen treatment, manual intervention is reduced, so that time can be saved, operation can be simplified, and operation errors can be reduced.
Description
Technical field
The invention belongs to biological sample process field, more particularly to a kind of device and method for processing slide glass sample.
Background technology
On slide glass sample albumen, pathogen or genetic test (such as immunohistochemistry and be originally hybrid gene detection
Deng) whole process needs tens steps of complexity, environment and manual operation various factors badly influence the standard of test result
True property.Streamline operation, reduction are to improve the task of top priority of Samples detection efficiency and accuracy by the factor of environment shadow.
At present in checkout procedure, slide glass sample is all that tiling is put, and is taken up space big, limits slide glass sample disposal
Flow and efficiency.
Reagent pipetting volume is from thin slice top injection sample-adding, and load position error is big.Unlimited incubation is easily caused after reagent is added dropwise
The problem of reagent volatilization dry plate.
Generally, reagent is that sample-adding is added dropwise from slide glass top, needs to remove original liquid on slide glass before sample-adding, drains
And wiping.Such operation human error is big, and Liquid Residue is more and affects the concentration and effect of next step reagent less.Wipe slide glass
Process, can also damage sample and the sample that above wipes of appearance is withered etc., cause sample disposal to fail.
The midway overwhelming majority sample of sample disposal process needs to heat.Needed during most sample disposals
Heat treatment, this is step necessary to making antigen or pathogen characteristic protein reply original shape or melting gene strand.Plus
Heat is typically carried out and maintained 3-60 minutes at a temperature of 80-121 DEG C.Most common method be pressure cooker boil, typically boiling boil,
Micro-wave oven boil or hot bath heating etc. method.However, the container or carrier of the conventional slide glass sample disposal of current manual operations
It is all thermo-labile, do not possess heatable function yet.Needing to be transferred to slide glass sample in the midway of slide glass sample disposal to heat
Container in carry out, then again need to be transferred on the operating desk of normal temperature, increased the complexity and operating error of operating process.
The present invention need not transfer, all the time treatment, slide glass sample disposal in same device during realizing slide glass sample
Overall process all without artificially take or shift slide glass, reduce manual intervention and intervention, you can time-consuming, simplify operation and can
Reduce operating error.
The content of the invention
In order to solve the above problems, it is proposed that a kind of device and method for processing slide glass sample, can make more large batch of
Slide glass closely places, slide glass is from start to finish carried out in one apparatus, ensure the slide glass sample moment obtains the covering of reagent, protects
The repeatability and reliability of card heat treatment.
The purpose of the present invention is realized at least through one of following technical scheme.
A kind of device for processing slide glass sample, it includes container, base, heater, slide glass cover plate, slide glass, slide glass branch
Frame, leakage fluid dram, inlet, controller, thermocouple, temperature display, soaking time display window and soaking time adjusting key；It is described
Slide mount assigns groove slide mount or multiple row by slot slide mount for single-row；
The slide glass cover plate coordinates with slide glass, and one group of composition is used for the slide glass component of sample disposal；
One or more slide mounts are hung on container；Each row on slide mount set more than one V-arrangement and assign
Groove, the V-arrangement of same row assigns groove linearly to arrange；Each V-arrangement is assigned and a spring leaf is set in groove；Outside slide glass component upper end
Shape assigns groove to match with V-arrangement, and slide glass component assigns groove to insert along the V-arrangement of slide mount, the back side compacting bullet of slide glass cover plate
Reed, produces spring pressure to compress slide glass and slide glass cover plate；
Heater is located at base top and positioned at container lower section；Heater of the controller control heater to container
Make；Thermocouple is placed in container；Thermocouple senses the temperature in container (301), and the temperature information transmission that will be sensed in real time
To controller to adjust the power output of heater, and then adjust temperature；Temperature in temperature display display container；Insulation
Time adjustment key can adjust the time for setting insulation；The display of soaking time display window needs the time of insulation；
Inlet allows heating medium or reagent of the storage in external container to be quantitatively adding in container when needed；Discharge opeing
Mouth is allowed the heating medium in container or liquid discharge container；Inlet and leakage fluid dram can be connected with Micropump or magnetic valve, by
Controller controls the folding of Micropump or magnetic valve.
Further, have a rectangular plane groove on the slide glass cover plate so that slide glass and cover plate press close to after it
Between formed a capillary gap；Slide glass sample sheet adhering in the capillary gap on the surface of slide glass, sample adding liquid by cover plate and
The loading slot formed between slide glass enters capillary gap, and uniform fold is on slide glass sample.
Further, the cover plate includes capillary plane, depth localization face, width locating piece, bottom locating piece, groove side
With channel opening face；The two sides top facing each other of the capillary plane is provided with described two depth localization faces, and capillary is put down
Face is parallel with depth localization face；The plane of the slide glass is fitted near the part of both sides of the edge with depth localization face, makes slide glass table
A capillary gap is formed between face and capillary plane；Outer ledge on each depth localization face away from capillary plane is provided with one
The width locating piece more than individual；Vertical range between width locating piece and slide glass on different depth locating surface
Width matches to play position-limiting action；Bottom locating piece is arranged on the bottom in depth localization face；Put down with capillary in the channel opening face
The upper end in face connects and forms included angle A 1, and A1 is 1~175 degree of plane angle；Channel opening face both sides are respectively connected with a groove side；Institute
State channel opening face and two groove sides and the loading slot of and the capillary gap area, capillary seam are collectively formed with slide glass plane
Gap bottom has slit aperture；Slide glass cover plate is suitable with the length of slide glass or equal, the side fitted with slide glass plane on groove side
The upper end in i.e. described two depth localization faces is equipped with a slot tag, and when label on slide glass, slot tag provides enough
Space be used to accommodate the thickness of label, enable slide glass cover plate and slide glass be adjacent to without by slide glass whether the shadow of label
Ring；Top striped is provided with the outside of two groove sides, for increasing the frictional force between groove side and finger；Capillary plane and depth
Vertical range between degree locating surface is 0.01~0.5mm, and slide surface is put down with capillary after being adjacent to slide glass cover plate and slide glass
One spacing of formation is the capillary gap of 0.01~0.5mm between face；The width locating piece is higher by the thickness in depth localization face
It is 0.1~1mm；Bottom locating piece is upward hook-shaped, and bottom locating piece determines that cover plate is pasted with slide glass together with width locating piece
Relative position after conjunction simultaneously assists to keep being brought into close contact between cover plate and slide glass.
Further, after slide glass component insertion V-arrangement assigns groove, slide glass is inclined or erects, the angle of slide glass and vertical direction
Spend is 1~90 degree.
Further, a biological sample is at least contained on slide glass.
Further, the two ends of slide mount are hung on container two sides facing each other, make the slide glass in slide mount
Gap is left with container bottom in component bottom；Integrally formed vertical or inclined multiple V-arrangements are provided with the slide mount
Groove is assigned, each V-arrangement is fixedly installed spring leaf in assigning groove；Spring leaf assigns groove to be integrated and is connected knot with the V-arrangement of slide mount
Structure or independent separate are set, and spring leaf is fixed on V-arrangement and is assigned in groove with chimeric or bonding way when using independent separate to set；
Assigned in groove when slide glass component assigns into V-arrangement, the surface pressing spring piece of cover plate, spring leaf plays the work for clamping slide glass and cover plate
With.
Further, including one or more independent containers, carrying out different temperatures, varying environment or different works simultaneously
Make the treatment of slide glass sample under flow.
Further, the heater uses electrical bar heating, electric-heating-wire-heating, heating using microwave, electromagnetic induction heating
Or thermal medium circulating-heating mode；The heater is conjoined structure or detachable structure with container.
In processing method using the device of above-mentioned treatment slide glass sample, what slide glass cover plate and slide glass were constituted after being adjacent to
Heated in the thermal capacitance liquid that slide glass component is completely soaked or bottom part is inserted in container, even if heating process produces volatilization,
Appearance liquid in gap can also be sucked from the slit aperture of bottom, be automatically replenished.
Further, when slide glass component is heat-treated, the heating medium in container is heated to boiling, reaches control heat treatment
Temperature.
Further, it is assumed that hold in container in checkout procedure after liquid seethes with excitement and require that soaking time is T2；When beginning to warm up,
Controller sets heating power for K1 is quickly heated；In heating process, thermocouple feeds back solution temperature at any time；Temperature reaches
During to boiling point, controller sets heating power as K2, keeps gentle boiling, and starts timing simultaneously, temporary when timing reaches T2
Stop heating.
Further, to after being heated to be by verification experimental verification of container, need to only set the time T1 that is heated to boiling and
Soaking time T2, time and power without adjusting heating by the feedback of thermocouple.
Further, controller automatically controls feed liquor Micropump, discharge opeing Micropump to container liquid filling and discharge opeing；At slide glass sample
When heat treatment is needed in reason, feed liquor Micropump is to liquid filling in container to carry out heating of boiling to slide glass sample；After the heat treatment,
Discharge opeing Micropump will heat the waste liquid extraction that liquid is extracted out or will produced during slide glass sample disposal.
Further, plus during novel agent, novel agent enters gap from loading slot, and original reagent is from slide glass component in gap
The slit aperture outflow of bottom, is replaced automatically；Plus without being purged the reagent of previous step, draining operation before novel agent.
Further, it is assumed that the flow velocity of feed liquor Micropump is V2, and the flow velocity of discharge opeing Micropump is V3, and the length of container is L, width
It is W；, it is necessary to the depth for increasing solution in a reservoir is H in an operating process, then new solubilization liquid can be in the controller set
The numerical value of depth is H, and now controller calculates the running time T 3=L*W*H/V2 of feed liquor Micropump automatically；When needing above-mentioned H
During the solution discharge container of depth, then the working time for having controller to automatically control discharge opeing Micropump sets for T3+ Δ t, Δ t are one
Fixed time margin, the liquid the purpose is to ensure in container can be discharged totally.
When the present invention carries out the heat treatment of slide glass component, the heating medium in container can be heated to boiling, by heating
The boiling point control heat treatment temperature of medium, reaches homogeneity, the reproducibility and reliability of heat treatment.The full mistake of slide glass sample disposal
Journey slide glass is on same position without movement.Slide glass component be heat-treated when, multiple slide glass components in same container by
Carried out at a temperature of the control of single heating controller, separately heated rather than each slide glass sample, temperature control alone.
The controller using the controller such as PIC single chip microcomputer, 51 single-chip microcomputers or PLC, by controller to after
The energization and power-off of electrical equipment are controlled, so as to realize the start and stop to heating function, realize to inlet port and outlet
The control of folding.
Relative to prior art, the present invention has advantages below and technique effect：
Slide glass component is formed when slide glass cover plate of the invention is combined with slide glass, there is a fine gap in slide glass component center,
Fine gap top has individual loading slot, reagent to be full of gap by gravity and capillary siphonage after being added to loading slot, unnecessary
Reagent from the bottom slit aperture outflow in fine gap, but the liquid in gap will not drain off, it is ensured that slide glass sample is tried
The covering of agent；When needing heat treatment, heated to irrigating reagent in container or heating medium, assigned into the slide glass assigned in groove
Component will whole or bottom part be immersed in the heating medium in container；Even if volatilization is produced in heating, also due to capillary
Siphonage, liquid can also be sucked by the slit aperture of slide glass component bottom in gap, be automatically replenished, and ensure heat treatment
Process will not occur the withered phenomenon of sample；Heat time can be by time adjustment window flexible modulation；Temperature in container is led to
Excess temperature display screen is observed at any time.The present invention can be such that the whole process of slide glass sample disposal carries out in one apparatus all the time,
Without midway transfer slide glass sample.Device of the invention is provided with container plus perfusion heating and is discharged at medium from container
The device of heating medium and waste liquid, to reduce need artificial operation and intervention in slide treatment process.
Further optimally, the present invention can be set multiple independent containers, can carry out temperature control independently, use
The treatment of the slide glass sample under carrying out different temperatures simultaneously or using different operating flow.
When this slide glass for designing component is tiltedly vertical or setting is put (between 1-90 degree), novel agent is loaded in operating process
When, the old reagent in gap is replaced by novel agent automatically, is reserved from the flow-guiding mouth of bottom, without plus novel agent before by slide glass
Upper original liquid (reagent) such as removes, drains at the step.The overall process of slide glass sample disposal all without artificially take or shift slide glass,
Improve the efficiency of cleaning and simplify flow.
Brief description of the drawings
Fig. 1 is the schematic diagram of slide glass specimen processing devices in example.
Fig. 2 is a kind of structural representation of slide glass cover plate in example.
Fig. 3 is the schematic diagram of a kind of slide glass and cover plate combination part (slide glass component) in example.
Fig. 4 a are the front views of slide glass component in Fig. 3；
Fig. 4 b are the A-A profiles of Fig. 3；
Fig. 4 c are the enlarged diagrams of the local B of Fig. 4 b.
Fig. 5 a are a kind of slide mount schematic diagrames in example.
Fig. 5 b are a kind of slide mount schematic diagrames for assigning groove containing multiple row in example.
Fig. 6 is a kind of slide mount schematic diagram for having assigned slide glass component in example.
Fig. 7 is to process slide glass sample device in example to remove schematic diagram after slide mount.
Fig. 8 is the sectional view of Fig. 7 shown devices.
Fig. 9 is the schematic diagram after Fig. 8 shown devices place slide mount and slide glass component.
Figure 10 is that Fig. 7 shown devices placement multiple row assigns the schematic diagram after the slide mount of groove.
In figure：301- containers；302- bases；303- heaters；307- leakage fluid drams；308- inlets；309- controllers；
310- discharge opeings Micropump (magnetic valve)；311- feed liquor Micropumps；312- electroheat pairs；313- temperature displays；314- soaking times show
Window；315- soaking time adjusting keys；316- feets；101- slide glasses；100- slide glass cover plates；200- slide mounts；201- template bullets
Spring；202- supports skew wall；203- presses slot.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described, but implementation of the invention and protection are not limited to
This.
As shown in Fig. 1~Figure 10, a kind of slide glass specimen processing devices of this example, mainly include container 301, base 302,
Heater 303, leakage fluid dram 307, inlet 308, controller 309, positive displacement pump 310, liquid feeding pump 311, electroheat pair 312, temperature show
The parts such as display screen 313, time adjustment window 314, slide glass cover plate 601, slide glass 101 and slide mount 602.
Such as Fig. 5 a, Fig. 5 b, the slide mount assigns groove slide mount 200 or multiple row by slot slide mount for single-row
200b。
As Fig. 3, the slide glass cover plate 100 coordinate with slide glass 101, one group of composition is used for the slide glass component 103 of sample disposal.
In Fig. 1, one or more slide mounts are hung on container 301；Each row on slide mount set more than one
V-arrangement assign groove 203, the V-arrangement of same row assigns groove 203 linearly to arrange；Each V-arrangement is assigned and a spring leaf is set in groove
201；The profile of slide glass component upper end assigns groove to match with V-arrangement, and slide glass component 103 assigns groove to insert along the V-arrangement of slide mount
Enter, the back side compacting spring leaf 201 of slide glass cover plate 100 produces spring pressure to compress slide glass 101 and slide glass cover plate 100.
Heater 303 is located at the top of base 302 and positioned at the lower section of container 301；Controller 309 controls heater to holding
The heating work of device 301；Thermocouple 312 is placed in container；Temperature in sensing container (301) in real time of thermocouple 312, and will
The temperature information of sensing passes to controller 309 to adjust the power output of heater, and then adjusts temperature；Temperature display
Temperature in 313 display containers；The adjustable time that insulation is set of soaking time adjusting key 315；Soaking time display window 314 shows
Show the time of needs insulation；
Inlet 308 allows heating medium or reagent of the storage in external container to be quantitatively adding container 301 when needed
In；Leakage fluid dram 307 is allowed the heating medium in container 301 or liquid discharge container；Inlet and leakage fluid dram can with Micropump or
Magnetic valve is connected, and the folding of Micropump or magnetic valve is controlled by controller 309.
Have a rectangular plane groove on the slide glass cover plate 100 so that slide glass 101 and cover plate press close to after between shape
Into a capillary gap；In the capillary gap on the surface of slide glass 101, sample adding liquid passes through cover plate 100 to slide glass sample sheet adhering
The loading slot 102 formed and slide glass 101 between enters capillary gap, and uniform fold is on slide glass sample.Due to gravity
With capillary siphonage, sample adding liquid is by one formed between cover plate and slide glass 101 loading slot 102 into capillary seam that is open
Gap, is covered on sample.
Such as Fig. 3 and Fig. 4 a~Fig. 4 c, the cover plate includes capillary plane 1, depth localization face 2, width locating piece 3, bottom
Locating piece 4, groove side 6 and channel opening face 7；The two sides top facing each other of the capillary plane 1 is provided with described two
Depth localization face 2, capillary plane 1 is parallel with depth localization face；Part and depth of the plane of the slide glass near both sides of the edge
Locating surface 2 is fitted, and makes to form a capillary gap between slide surface and capillary plane 1；Away from hair on each depth localization face 2
The outer ledge of thin plane 1 is provided with the more than one width locating piece 3；Width on different depth locating surface 2 is determined
Vertical range between the block 3 of position matches to play position-limiting action with the width of slide glass；Bottom locating piece 4 is arranged on depth localization face
2 bottom；The channel opening face 7 is connected with the upper end of capillary plane 1 and forms included angle A 1, and A1 is 1~175 degree of plane angle；Groove
The both sides of opening surface 7 are respectively connected with a groove side 6；The channel opening face 7 and two groove sides 6 collectively form one with slide glass plane
The individual loading slot with the capillary gap area, capillary gap bottom has slit aperture；Slide glass cover plate is suitable with the length of slide glass
Or it is equal, the upper end in the i.e. described two depth localization faces 2 in the side fitted with slide glass plane on groove side 6 is equipped with a label
Groove 9, when label on slide glass, slot tag provides enough spaces is used to accommodate the thickness of label, enables slide glass cover plate
It is adjacent to without by whether label is influenceed on slide glass with slide glass；The outside of two groove sides 6 is provided with top striped 10, is used for
Increase the frictional force between groove side 6 and finger；Vertical range between capillary plane 1 and depth localization face 2 for 0.01~
0.5mm, formed between slide surface and capillary plane 1 after being adjacent to slide glass cover plate and slide glass spacing be 0.01~
The capillary gap of 0.5mm；The thickness that the width locating piece 3 is higher by depth localization face 2 is 0.1~1mm；Bottom locating piece 4 is
Upward is hook-shaped, bottom locating piece 4 determine together with width locating piece 3 cover plate fitted with slide glass after relative position and assist
Keep being brought into close contact between cover plate and slide glass.V-arrangement assigns the respective outer side edges of groove and slide glass component, container is accommodated more
Slide glass component, need to only clamp top striped position and the slide glass of two groove sides with finger when using, easily insert slide glass component
In entering to assign groove, it is to avoid unbalance stress or Impact direction have the deviation to cause slide glass to disengage slide glass cover plate during pressing slide glass top, protect
The formation in barrier capillary gap and accuracy, operate more simple and efficient.In addition, the arrangement of slide glass component can be entered with even closer
One step reduces space, improves treatment effeciency.
As example, all component it is indeformable using being resistant to more than 80 DEG C or soften material manufacture.Slide glass
After the insertion V-arrangement of component 103 assigns groove, slide glass is inclined or erects, and slide glass is 1~90 degree with the angle of vertical direction.On slide glass
At least contain a biological sample.
As Fig. 9, the two ends of slide mount are hung on container two sides facing each other, make the slide glass component in slide mount
Gap is left with container bottom in bottom；Integrally formed vertical or inclined multiple V-arrangements are provided with the slide mount to assign
Groove 203, each V-arrangement is fixedly installed spring leaf 201 in assigning groove 203；Spring leaf 201 and the V-arrangement of slide mount assign the groove 203 to be
Integral connecting structure or independent separate are set, and spring leaf 201 is fixed with chimeric or bonding way when using independent separate to set
Assigned in groove 203 in V-arrangement；Assigned in groove 203 when slide glass component assigns into V-arrangement, the surface pressing spring piece 201 of cover plate 100, bullet
Reed plays a part of to clamp slide glass 101 and cover plate 100.
A kind of device for the treatment of slide glass sample of this example can include one or more independent containers, to carry out simultaneously
Different temperatures, varying environment or different operating flow download the treatment of piece sample.The heater is using electrical bar heating, electricity
Heated filament heating, heating using microwave, electromagnetic induction heating or thermal medium circulating-heating mode；The heater 303 is company with container 301
Body structure or detachable structure.
Using a kind of processing method of the device for the treatment of slide glass sample of this example：Slide glass cover plate and slide glass be adjacent to after institute
Heated in the thermal capacitance liquid that the slide glass component 103 of composition is completely soaked or bottom part is inserted in container, even if heating process
Volatilization is produced, the appearance liquid in gap can also be sucked from the slit aperture of bottom, be automatically replenished.When slide glass component is heat-treated, can
Boiling is heated to by by the heating medium in container, control heat treatment temperature is reached.
It is assumed that requiring that soaking time is T2 after holding liquid boiling in checkout procedure in container 301；When beginning to warm up, controller
Heating power is set for K1 is quickly heated；In heating process, thermocouple (312) feeds back solution temperature at any time；Temperature reaches
During to boiling point, controller sets heating power as K2, keeps gentle boiling, and starts timing simultaneously, temporary when timing reaches T2
Stop heating.
As example to after being heated to be by verification experimental verification of container, need to only set the time T1 and guarantor for being heated to boiling
Warm time T2, time and power without adjusting heating by the feedback of thermocouple.
In example, plus during novel agent, novel agent enters gap from loading slot, and original reagent is from slide glass component bottom in gap
The slit aperture outflow in portion, is replaced automatically；Plus without being purged the reagent of previous step, draining operation before novel agent.Make
It is example, the controller uses PIC single chip microcomputer, the controller such as 51 single-chip microcomputers or PLC, by controller to relay
The energization and power-off of device control the startup of feed liquor and discharge opeing Micropump in stopping, controlling the startup of heater in stopping and add
Power output in thermal process.
Controller automatically controls feed liquor Micropump, discharge opeing Micropump to container liquid filling and discharge opeing；Needed when in slide glass sample disposal
During heat treatment, feed liquor Micropump is to liquid filling in container to carry out heating of boiling to slide glass sample；After the heat treatment, discharge opeing Micropump
The waste liquid extraction that liquid is extracted out or will produced during slide glass sample disposal will be heated.It is assumed that the flow velocity of feed liquor Micropump is V2, discharge opeing
The flow velocity of Micropump is V3, and the length of container is L, width is W；, it is necessary to increase solution in a reservoir in an operating process
Depth is H, then the numerical value that can in the controller set newly-increased solution deep is H, and now controller calculates feed liquor Micropump automatically
(311) running time T 3=L*W*H/V2；When needing for the solution of above-mentioned H depth to discharge container, then there is controller automatic
It is a time margin for setting for T3+ Δ t, Δ t to control working time of discharge opeing Micropump 310, the purpose is in ensuring container
Liquid can discharge totally.
When the present invention carries out the heat treatment of slide glass component, the heating medium in container can be heated to boiling, by heating
The boiling point control heat treatment temperature of medium, reaches homogeneity, the reproducibility and reliability of heat treatment.The full mistake of slide glass sample disposal
Journey slide glass is on same position without movement.Slide glass component be heat-treated when, multiple slide glass components in same container by
Carried out at a temperature of the control of single heating controller, separately heated rather than each slide glass sample, temperature control alone.
The announcement and teaching of book according to the above description, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula is changed and changed.Therefore, the invention is not limited in specific embodiment disclosed and described above, to of the invention
Some modifications and changes should also be as falling into scope of the claims of the invention.
Claims (15)
1. it is a kind of process slide glass sample device, it is characterised in that including container（301）, base（302）, heater（303）、
Slide glass cover plate（100）, slide glass（101）, slide mount, leakage fluid dram（307）, inlet（308）, controller（309）, thermocouple
（312）, temperature display（313）, soaking time display window（314）With soaking time adjusting key（315）；The slide mount is
It is single-row to assign groove slide mount（200）Or multiple row presses slot slide mount（200b）；
The slide glass cover plate（100）With slide glass（101）Coordinate, one group of composition is used for the slide glass component of sample disposal（103）；
Container（301）Upper one or more slide mounts of suspension；Each row on slide mount set more than one V-arrangement and assign
Groove（203）, the V-arrangement of same row assigns groove（203）Linearly arrange；Each V-arrangement is assigned and a spring leaf is set in groove（201）；
The profile of slide glass component upper end assigns groove to match with V-arrangement, slide glass component（103）Groove is assigned to insert along the V-arrangement of slide mount,
Slide glass cover plate（100）The back side compacting spring leaf（201）, produce spring pressure to compress slide glass（101）With slide glass cover plate（100）；
Heater（303）Positioned at base（302）Top and positioned at container（301）Lower section；Controller（309）Control heater
To container（301）Heating work；Thermocouple（312）It is placed in container；Thermocouple（312）In real time in sensing container (301)
Temperature, and the temperature information of sensing is passed into controller（309）To adjust the power output of heater, and then adjust temperature
Degree；Temperature display（313）Temperature in display container；Soaking time adjusting key（315）The adjustable time that insulation is set；Protect
Warm time showing window（314）Display needs the time of insulation；
Inlet（308）Heating medium or reagent of the storage in external container is allowed to be quantitatively adding container when needed（301）
In；Leakage fluid dram（307）Allow container（301）In heating medium or liquid discharge container；Inlet and leakage fluid dram can with it is micro-
Pump or magnetic valve are connected, by controller（309）The folding of control Micropump or magnetic valve.
2. it is according to claim 1 it is a kind of process slide glass sample device, it is characterised in that the slide glass cover plate（100）On
There is a rectangular plane groove so that slide glass（101）And cover plate press close to after between formed a capillary gap；Slide glass sample
Sheet adhering slide glass in capillary gap（101）Surface on, sample adding liquid pass through cover plate（100）And slide glass（101）Between formed
A loading slot（102）Into capillary gap, and uniform fold is on slide glass sample.
3. a kind of device for processing slide glass sample according to claim 2, it is characterised in that the cover plate is flat including capillary
Face（1）, depth localization face（2）, width locating piece（3）, bottom locating piece（4）, groove side（6）With channel opening face（7）；The hair
Thin plane（1）Two sides top facing each other be provided with described two depth localization faces（2）, capillary plane（1）With depth
Locating surface is parallel；Part and depth localization face of the plane of the slide glass near both sides of the edge（2）Laminating, makes slide surface and hair
Thin plane（1）Between formed a capillary gap；Each depth localization face（2）On away from capillary plane（1）Outer ledge set
There is the more than one width locating piece（3）；Positioned at different depth locating surface（2）On width locating piece（3）Between hang down
Straight distance matches to play position-limiting action with the width of slide glass；Bottom locating piece（4）The depth localization face of being arranged on（2）Bottom；
The channel opening face（7）With capillary plane（1）Upper end connect and form included angle A 1, A1 be 1 ~ 175 degree of plane angle；Channel opening face
（7）Both sides are respectively connected with a groove side（6）；The channel opening face（7）And two groove sides（6）Collectively formed with slide glass plane
One with the loading slot of the capillary gap area, capillary gap bottom has slit aperture；The length phase of slide glass cover plate and slide glass
When or equal, groove side（6）The upper i.e. described two depth localization faces in side fitted with slide glass plane（2）Upper end be equipped with one
Individual slot tag（9）, when label on slide glass, slot tag provides enough spaces is used to accommodate the thickness of label, makes slide glass
Cover plate can be adjacent to without by whether label is influenceed on slide glass with slide glass；Two groove sides（6）Outside be provided with top
Striped（10）, for increasing groove side（6）Frictional force between finger；Capillary plane（1）With depth localization face（2）Between
Vertical range is 0.01 ~ 0.5mm, slide surface and capillary plane after being adjacent to slide glass cover plate and slide glass（1）Between form one
Individual spacing is the capillary gap of 0.01 ~ 0.5mm；The width locating piece（3）The depth localization face of being higher by（2）Thickness for 0.1 ~
1mm；Bottom locating piece（4）It is upward hook-shaped, bottom locating piece（4）With width locating piece（3）Cover plate and slide glass are determined together
Relative position after laminating simultaneously assists to keep being brought into close contact between cover plate and slide glass.
4. it is according to claim 1 it is a kind of process slide glass sample device, it is characterised in that：Slide glass component（103）Insertion V
After shape assigns groove, slide glass is inclined or erects, and slide glass is 1 ~ 90 degree with the angle of vertical direction.
5. it is according to claim 1 it is a kind of process slide glass sample device, it is characterised in that：At least contain one on slide glass
Biological sample.
6. it is according to claim 1 it is a kind of process slide glass sample device, it is characterised in that：The two ends of slide mount hang over
On container two sides facing each other, the slide glass component bottom in slide mount is set to leave gap with container bottom；The slide glass
Integrally formed vertical or inclined multiple V-arrangements are provided with support and assign groove（203）, each V-arrangement assigns groove（203）Middle fixation
Spring leaf is set（201）；Spring leaf（201）V-arrangement with slide mount assigns groove（203）It is integrated attachment structure or independent point
From setting, spring leaf when being set using independent separate（201）It is fixed on V-arrangement and assigns groove with chimeric or bonding way（203）It is interior；When
Slide glass component assigns into V-arrangement and assigns groove（203）In, cover plate（100）Surface pressing spring piece（201）, spring leaf plays clamping
Slide glass（101）With cover plate（100）Effect.
7. it is according to claim 1 it is a kind of process slide glass sample device, it is characterised in that：Including one or more independences
Container, download the treatment of piece sample to carry out different temperatures, varying environment or different operating flow simultaneously.
8. a kind of device for processing slide glass sample according to claim 1, it is characterised in that the heater is using electricity
Hot pin heating, electric-heating-wire-heating, heating using microwave, electromagnetic induction heating or thermal medium circulating-heating mode；The heater（303）
With container（301）It is conjoined structure or detachable structure.
9. using a kind for the treatment of slide glass sample described in any one of claim 1 ~ 8 device processing method, it is characterised in that：
The slide glass component that slide glass cover plate and slide glass are constituted after being adjacent to（103）Be completely soaked or bottom part insertion container in thermal capacitance
Heated in liquid, even if heating process produces volatilization, the appearance liquid in gap can also be sucked from the slit aperture of bottom, obtain automatic
Supplement.
10. processing method according to claim 9, it is characterised in that：When slide glass component is heat-treated, by the heating in container
Medium is heated to boiling, reaches control heat treatment temperature.
11. processing methods according to claim 9, it is characterised in that：It is assumed that container in checkout procedure（301）Middle appearance liquid boiling
Require that soaking time is T2 after rising；When beginning to warm up, controller sets heating power for K1 is quickly heated；Heated
Cheng Zhong, thermocouple (312) feeds back solution temperature at any time；When temperature reaches boiling point, controller sets heating power as K2, keeps temperature
And boiling, and start timing simultaneously, heating is suspended when timing reaches T2.
12. processing methods according to claim 9, it is characterised in that：To after being heated to be by verification experimental verification of container,
The time T1 and soaking time T2 for being heated to boiling need to be only set, time and work(without adjusting heating by the feedback of thermocouple
Rate.
13. processing methods according to claim 9, it is characterised in that：
Controller automatically controls feed liquor Micropump, discharge opeing Micropump to container liquid filling and discharge opeing；When needing hot place in slide glass sample disposal
During reason, feed liquor Micropump is to liquid filling in container to carry out heating of boiling to slide glass sample；After the heat treatment, discharge opeing Micropump will add
The waste liquid extraction that hydrothermal solution is extracted out or will produced during slide glass sample disposal.
14. processing methods according to claim 9, it is characterised in that：Plus during novel agent, novel agent enters seam from loading slot
Gap, original reagent flows out from the slit aperture of slide glass component bottom in gap, is replaced automatically；Plus need not will be upper before novel agent
The reagent of one step is purged, drains operation.
15. processing methods according to claim 13, it is characterised in that：It is assumed that the flow velocity of feed liquor Micropump is V2, discharge opeing is micro-
The flow velocity of pump is V3, and the length of container is L, width is W；, it is necessary to increase the depth of solution in a reservoir in an operating process
It is H to spend, then the numerical value that can in the controller set newly-increased solution deep is H, and now controller calculates feed liquor Micropump automatically（311）
Running time T 3=L*W*H/V2；When needing for the solution of above-mentioned H depth to discharge container, then there is controller to automatically control row
The working time of liquid Micropump (310) is that T3+ Δ t, Δ t are a time margin for setting, the purpose is to ensure the liquid in container
Body can be discharged totally.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610798084.7A CN106706379B (en) | 2016-08-31 | 2016-08-31 | Device and method for processing slide specimen |
| CA3018623A CA3018623A1 (en) | 2016-03-21 | 2017-03-21 | Device and method for processing slide specimen thereof |
| JP2019500721A JP6694224B2 (en) | 2016-03-21 | 2017-03-21 | Sample slide processing apparatus and processing method |
| PCT/CN2017/077571 WO2017162149A1 (en) | 2016-03-21 | 2017-03-21 | Device and method of processing sample slide |
| EP17769426.2A EP3435063A4 (en) | 2016-03-21 | 2017-03-21 | Device and method of processing sample slide |
| US16/087,106 US10830679B2 (en) | 2016-03-21 | 2017-03-21 | Device and method for processing slide specimen thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610798084.7A CN106706379B (en) | 2016-08-31 | 2016-08-31 | Device and method for processing slide specimen |
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| CN106706379A true CN106706379A (en) | 2017-05-24 |
| CN106706379B CN106706379B (en) | 2023-09-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610798084.7A Active CN106706379B (en) | 2016-03-21 | 2016-08-31 | Device and method for processing slide specimen |
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