CN105675383A - Multichannel slide specimen incubation device having heating function - Google Patents
Multichannel slide specimen incubation device having heating function Download PDFInfo
- Publication number
- CN105675383A CN105675383A CN201610160688.9A CN201610160688A CN105675383A CN 105675383 A CN105675383 A CN 105675383A CN 201610160688 A CN201610160688 A CN 201610160688A CN 105675383 A CN105675383 A CN 105675383A
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- China
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- slide glass
- heating
- multichannel
- discharge outlet
- heating function
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- 238000010438 heat treatment Methods 0.000 title claims abstract description 45
- 238000011534 incubation Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 230000008569 process Effects 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 claims abstract description 5
- 239000011521 glass Substances 0.000 claims description 61
- 239000000523 sample Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 4
- 239000012472 biological sample Substances 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims description 3
- 239000012774 insulation material Substances 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 2
- 238000001125 extrusion Methods 0.000 claims description 2
- 230000006698 induction Effects 0.000 claims description 2
- 238000007669 thermal treatment Methods 0.000 abstract 3
- 238000005485 electric heating Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000010412 perfusion Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229910000639 Spring steel Inorganic materials 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000013536 elastomeric material Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a multichannel slide specimen incubation device having a heating function. The multichannel slide specimen incubation device mainly comprises slides, cover plates, inserting frame tanks, a heater container, supporting feet, a heating plate, an electric heating pipe, cover plate supports, spring pieces, an electromagnet, an upper water outlet and a lower water outlet. The slides are erected or obliquely inserted other than being flatwise laid to save slide specimen arrangement space, and more slide specimens can be treated. When the slide specimens need to be heated in the treatment process, liquid is directly filled to the inserting frame tanks for heating, and the slide specimens do not need to be transferred during thermal treatment any more, so that the slide specimens are treated in a same container from beginning to end; during thermal treatment, even if volatilization occurs, liquid in gaps can be automatically replenished due to capillary effect, so that thermal treatment is sufficient and uniform and drying-up is avoided. The multichannel slide specimen incubation device is simpler and more reliable to operate, larger in specimen treatment quantity and convenient for implementing full-automatic operation process.
Description
Technical field
The invention belongs to biological sample process field, particularly relate to a kind of multichannel slide glass specimen incubating device with heating function.
Background technology
The whole process of immunohistochemistry needs nearly 30 steps, hatch including: a) dewaxing, aquation, endogenous enzyme blocking-up, cleaning, high-temperature hot reparation, cooling, cleaning, primary antibodie, clean, two resist hatch, clean, synergist is hatched, clean, develop the color, clean, serve as a contrast the steps such as dye, cleaning, dehydration, mounting. Impact by the technical merit between hospital and hospital and operating habit difference, it is difficult to standardization. The efficiency and the reliability that improve sample disposal become the task of top priority.
At present in checkout procedure, reagent pipetting volume sprays application of sample above thin slice, and load position error is big, and reagent covering in section is with randomness, it is difficult to guaranteeing to cover whole section, some specimen do not obtain the covering of reagent and false negative occur. Needing high-temperature heating in sample disposal process, this is to make antigen or pathogen characteristic protein reply step necessary to original shape. Heating requirements reaches the temperature of 80-100 DEG C or higher and maintains 5-40 minute. Most common method is that pressure cooker boils, generally boils and boil or the method such as hot water temperature bath.
But, the container of at present conventional slide glass sample disposal or carrier do not possess and can heat or the resistance to performance being heated. Need in the midway of slide glass sample disposal to transfer in heatable container slide glass specimen to carry out, then need again to transfer on the operating board of room temperature, add complexity and the operating error of operating process.
Realize slide glass specimen to be heated in position processing and just can simplify slide glass specimen processing operation step and be easier to carry out full-automatic operation flow process, meet the requirement of simpler reliable, the higher efficiency of slide glass sample disposal.
Summary of the invention
In order to solve the problems referred to above, propose a kind of multichannel slide glass specimen incubating device with heating function, more large batch of section can be processed simultaneously and improve section response time in liquid reagent and operation to save the time further and to improve efficiency, and fundamentally ensureing the reproducibility and reliability of hot repair process.
A kind of multichannel slide glass specimen incubating device with heating function, it includes slide glass, cover plate, bay groove, heater canister, spike, lower discharge outlet, hot plate, electrothermal tube, cover board support, spring leaf, electric magnet, upper water inlet;
Described cover board support is intervally arranged by array format and is fixed in bay groove; Each cover board support is fixed with spring leaf; The molectron that slide glass and cover plate coordinate, inserts along the gap between cover board support and spring leaf, the surface extrusion spring sheet of cover plate, and spring leaf produces spring pressure and is applied on slide glass and cover plate and realizes pressuring action;
Adding heat pipe and be embedded in hot plate, hot plate is arranged in heater canister; Add heat pipe to be connected with external power supply, and controlled power and the time of heating by manual or controller; Add heat produced by heat pipe and pass to hot plate; Bay trench bottom or main body are made of metal; During energising, the heating heat pipe of bottom is operationally placed on hot plate, accepts the heat of hot plate to heat the liquid in bay groove.
Further, bay groove is provided with discharge outlet, and heater canister is provided with lower discharge outlet; Upper discharge outlet, lower discharge outlet are carried out discharge opeing by manual operation.
Further, bay groove is provided with discharge outlet, and heater canister is provided with lower discharge outlet; Upper discharge outlet, lower discharge outlet are controlled discharge opeing by electric device (such as electromagnetic valve).
Further, bay groove can arranging string or multiple row cover board support, thus inserting the slide glass assembly that string or multiple row are adjacent to by slide glass cover plate and slide glass, forming array type close arrangement.
Further, a biological sample is at least contained above described slide glass.
Further, the material used is capable of withstanding by higher temperature, is generally possible to tolerance 80oMore than C and indeformable or softening.
Further, described device is provided with the heater of Contiuum type or separate type.
Further, the mode of heating of described hot plate, adopt disjunctor or separate type heating rod, heating plate, Electromagnetic Heating induction system to be heated, or heat container irrigates hot liquid carry out temperature bath heating to adding.
Further, when slide glass sample disposal process needs heat treatment, in bay groove, directly irrigate liquid and be heated, it is not necessary to slide glass specimen being shifted in midway, the process of slide glass specimen from start to finish carries out in a container.
Further, heater canister is provided with a spike; Heater canister is made up of insulation material, plays the effect of insulation.
Relative to prior art, the present invention has the following advantages and technique effect:
Assembly of the invention enables slide glass specimen in the way of setting or oblique angle setting rather than the mode of tiling is placed, it is possible to the array type close structure of multiple rows of multiple row is arranged; Slide glass assembly is when erectting, and the liquid in fine gap will not drain off, it is ensured that the slide glass specimen moment obtains the covering of reagent; Section preparation can directly soak or insert in hot solution and uniformly heat, even if the liquid in gap produces volatilization in heating process also due to capillarity is automatically replenished from the fine slit aperture suction of slide glass component bottom, heating is fully, do not produce the phenomenon dried up, ensure the reproducibility and reliability of hot repair process; In assembly of the invention, all of accessory all uses heat proof material to make, and disjunctor is set or separable heater, as long as perfusion appearance liquid just can be made directly heating in bay groove, slide glass specimen is made from start to finish to carry out in a container in processing procedure.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of multichannel slide glass specimen incubating device with heating function of the present invention.
Fig. 2 is the generalized section of Fig. 1.
Fig. 3 is the structural representation of the heated base part of the present invention.
Fig. 4 is the structural representation of the bay groove of the present invention.
Fig. 5 is the generalized section of Fig. 4.
Fig. 6 is the structural representation of the slide glass of the present invention and cover plate.
Fig. 7 a is the front view of slide glass assembly in Fig. 6;
Fig. 7 b is the A-A profile of Fig. 7 a;
Fig. 7 c is the enlarged diagram of the local B of Fig. 7 b.
Fig. 8 is one and assigns and arrange multiple row slide glass in tank therefor and assign groove schematic diagram.
In figure: 1-slide glass; 2-cover plate; 3-bay groove; 4-heater canister; 5-props up spike; Discharge outlet under 6-; 7-hot plate; 8-electrothermal tube; 9-cover board support; 10-spring leaf; 11-electric magnet; The upper water inlet of 12-and lower discharge outlet.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described, but is not limited to this.
As shown in Fig. 1 ~ Fig. 7, a kind of multichannel slide glass specimen incubating device with heating function, mainly includes the parts such as slide glass 1, cover plate 2, bay groove 3, heater canister 4, spike 5, lower discharge outlet 6, hot plate 7, electrothermal tube 8, cover board support 9, spring leaf 10, electric magnet 11, upper discharge outlet 12.
Described cover board support 9 is fixed in bay groove 3, and array at a certain distance; Each cover board support 9 is fixed with spring leaf 10; The molectron that slide glass 1 and cover plate 2 coordinate, inserts along the gap between cover board support 9 and spring leaf 10, the surface compacting spring leaf 10 of cover plate 2; Spring leaf 10, adopts sheet copper, spring steel or other elastomeric material and elastic construction to make, and produces spring pressure and is applied on slide glass 1 and cover plate 2 with both compressions.
As Fig. 6, Fig. 7 a ~ Fig. 7 c, slide glass 1 and cover plate 2 form one group of slide glass assembly for sample disposal, cover plate 2 has capillary plane, forms a capillary gap between slide surface and capillary plane; Described channel opening face 601 is connected with capillary plane and forms angle is 1 ~ 175 degree of plane angle.
Adding heat pipe 8 and be embedded in hot plate 7, hot plate is arranged in heater canister 4; Add heat pipe 8 to be connected with external power supply, and be not drawn into controlling power and the time of heating by controller; Add heat produced by heat pipe 8 and pass to hot plate 7. Bay groove 3 is made of metal, and is operationally placed on hot plate 7, accepts the heat of hot plate 7 to heat the liquid in bay groove 3.
Further, bay groove 3 is provided with electric magnet 11, and when device is in heated condition, controller (not shown) controls electric magnet 11 and connects generation electromagnetic force, holds hot plate 7 and makes hot plate 7 improve heat conduction efficiency with bay groove 3 close contact.
Further, the bottom of bay groove 3 is provided with discharge outlet 12, and heater canister 4 is provided with lower discharge outlet 6, and upper discharge outlet 12 is coaxial with lower discharge outlet 6; Upper discharge outlet 12 is provided with electromagnetism valve block () in figure slightly, can by the opening and closing of discharge outlet 12 in controller () control in figure slightly.
Further, heater canister 4 is provided with a spike 5; Heater canister 4 is made up of insulation material, plays the effect of insulation.
Further, each slide glass bay groove 3 can arrange string or multiple row cover board support, such that it is able to arrange installation string or multiple row slide glass specimen.
Further, the material used is capable of withstanding by higher temperature, is generally possible to tolerance 80oMore than C and indeformable or fusing.
Further, the mode of heating of described hot plate 7, it is also possible to adopt Electromagnetic Heating or the similar method with container heating function and adopt the mode of perfusion hot liquid to be heated.
Further, described controller adopts the controllers such as PIC single chip microcomputer, 51 single-chip microcomputers or PLC.
Such as Fig. 8, bay groove can arranging string or multiple row cover board support, thus inserting the slide glass assembly that string or multiple row are adjacent to by slide glass cover plate and slide glass, forming array type close arrangement.
According to announcement and the instruction of description above, above-mentioned embodiment can also be modified and revise by those skilled in the art in the invention. Therefore, the invention is not limited in detailed description of the invention disclosed and described above, should also be as some modifications and changes of the present invention falling in the scope of the claims of the present invention.
Claims (10)
1. a multichannel slide glass specimen incubating device with heating function, it is characterised in that include slide glass (1), cover plate (2), bay groove (3), heater canister (4), spike (5), lower discharge outlet (6), hot plate (7), electrothermal tube (8), cover board support (9), spring leaf (10), electric magnet (11), upper water inlet (12);
Described cover board support (9) is intervally arranged by array format and is fixed in bay groove (3); Each cover board support (9) is fixed with spring leaf (10); The molectron that slide glass (1) and cover plate (2) coordinate, insert along the gap between cover board support (9) and spring leaf (10), surface extrusion spring sheet (10) of cover plate (2), spring leaf (10) produces spring pressure and is applied on slide glass (1) and cover plate (2) and realizes pressuring action;
Adding heat pipe (8) and be embedded in hot plate (7), hot plate is arranged in heater canister (4); Add heat pipe (8) to be connected with external power supply, and controlled power and the time of heating by manual or controller; Add heat produced by heat pipe (8) and pass to hot plate (7); Bay groove (3) bottom or main body are made of metal; During energising, the heating heat pipe (8) of bottom is operationally placed on hot plate (7), accepts the heat of hot plate (7) to heat the liquid in bay groove (3).
2. a kind of multichannel slide glass specimen incubating device with heating function according to claim 1, it is characterised in that: bay groove (3) is provided with discharge outlet (12), and heater canister is provided with lower discharge outlet (6); Upper discharge outlet (12), lower discharge outlet (6) are carried out discharge opeing by manual operation.
3. a kind of multichannel slide glass specimen incubating device with heating function according to claim 1, it is characterised in that: bay groove (3) is provided with discharge outlet (12), and heater canister is provided with lower discharge outlet (6); Upper discharge outlet (12), lower discharge outlet (6) are controlled discharge opeing by electric device.
4. a kind of multichannel slide glass specimen incubating device with heating function according to claim 1, it is characterized in that: bay groove (3) can arrange string or multiple row cover board support, thus inserting the slide glass assembly that string or multiple row are adjacent to by slide glass cover plate and slide glass, form array type close arrangement.
5. a kind of multichannel slide glass specimen incubating device with heating function according to claim 1, it is characterised in that: described slide glass (1) at least contains a biological sample above.
6. a kind of multichannel slide glass specimen incubating device with heating function according to claim 1, it is characterised in that: the material used is capable of withstanding by higher temperature, is generally possible to tolerance 80oMore than C and indeformable or softening.
7. a kind of multichannel slide glass specimen incubating device with heating function according to claim 1, it is characterised in that: described device is provided with the heater of Contiuum type or separate type.
8. a kind of multichannel slide glass specimen incubating device with heating function according to claim 1, it is characterized in that: the mode of heating of described hot plate (7), adopt disjunctor or separate type heating rod, heating plate, Electromagnetic Heating induction system to be heated, or heat container irrigates hot liquid carry out temperature bath heating to adding.
9. a kind of multichannel slide glass specimen incubating device with heating function according to claim 1, it is characterized in that: when slide glass sample disposal process needs heat treatment, in bay groove, directly irrigate liquid and be heated, without slide glass specimen being shifted in midway, the process of slide glass specimen from start to finish carries out in a container.
10. a kind of multichannel slide glass specimen incubating device with heating function according to claim 1, it is characterised in that: heater canister (4) is provided with a spike (5); Heater canister (4) is made up of insulation material, plays the effect of insulation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610160688.9A CN105675383B (en) | 2016-03-21 | 2016-03-21 | A kind of multichannel slide glass sample incubating device with heating function |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610160688.9A CN105675383B (en) | 2016-03-21 | 2016-03-21 | A kind of multichannel slide glass sample incubating device with heating function |
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| CN105675383A true CN105675383A (en) | 2016-06-15 |
| CN105675383B CN105675383B (en) | 2019-06-14 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106153436A (en) * | 2016-08-31 | 2016-11-23 | 广州深达生物制品技术有限公司 | A kind of annealing device of slide glass Samples detection |
| CN106706379A (en) * | 2016-08-31 | 2017-05-24 | 广州深达生物制品技术有限公司 | Device and method for treating slide specimens |
| CN106769382A (en) * | 2016-11-18 | 2017-05-31 | 广州深达生物制品技术有限公司 | The pretreatment unit and method of a kind of slide glass Samples detection |
| CN107144698A (en) * | 2017-06-27 | 2017-09-08 | 珠海意动智能装备有限公司 | Test reaction device, chip-detecting apparatus and its control method |
| WO2017162149A1 (en) * | 2016-03-21 | 2017-09-28 | 广州深达生物制品技术有限公司 | Device and method of processing sample slide |
| CN109421192A (en) * | 2017-08-31 | 2019-03-05 | 无锡拓利普科技有限公司 | Electric boiling plate component and the vulcanizer for using the component |
| CN106353516B (en) * | 2016-08-31 | 2019-07-23 | 厦门通灵生物医药科技有限公司 | A kind of device and method of full automatic treatment biology slide sample |
| CN114354317A (en) * | 2022-01-14 | 2022-04-15 | 厦门通灵生物医药科技有限公司 | Convertible support and detection device thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US10830679B2 (en) | 2016-03-21 | 2020-11-10 | Xiamen Talent Biomedical Technology Company, Ltd. | Device and method for processing slide specimen thereof |
| CN106153436A (en) * | 2016-08-31 | 2016-11-23 | 广州深达生物制品技术有限公司 | A kind of annealing device of slide glass Samples detection |
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| CN106353516B (en) * | 2016-08-31 | 2019-07-23 | 厦门通灵生物医药科技有限公司 | A kind of device and method of full automatic treatment biology slide sample |
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| CN107144698A (en) * | 2017-06-27 | 2017-09-08 | 珠海意动智能装备有限公司 | Test reaction device, chip-detecting apparatus and its control method |
| CN107144698B (en) * | 2017-06-27 | 2018-06-26 | 珠海意动智能装备有限公司 | Test reaction device, chip-detecting apparatus and its control method |
| CN109421192A (en) * | 2017-08-31 | 2019-03-05 | 无锡拓利普科技有限公司 | Electric boiling plate component and the vulcanizer for using the component |
| CN114354317A (en) * | 2022-01-14 | 2022-04-15 | 厦门通灵生物医药科技有限公司 | Convertible support and detection device thereof |
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