CN105675383B - A kind of multichannel slide glass sample incubating device with heating function - Google Patents
A kind of multichannel slide glass sample incubating device with heating function Download PDFInfo
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- CN105675383B CN105675383B CN201610160688.9A CN201610160688A CN105675383B CN 105675383 B CN105675383 B CN 105675383B CN 201610160688 A CN201610160688 A CN 201610160688A CN 105675383 B CN105675383 B CN 105675383B
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- slide glass
- glass sample
- heating
- cover board
- discharge outlet
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
Abstract
The present invention discloses a kind of multichannel slide glass sample incubating device with heating function, mainly includes the components such as slide glass, cover board, insert rack slot, heater canister, support leg, heating plate, electrothermal tube, cover board support, spring leaf, electromagnet, upper discharge outlet, lower discharge outlet.Erect present invention employs slide glass or oblique cutting rather than the mode that lays flat saves the space that slide glass sample is put, be capable of handling more large batch of slide glass sample；It is directly heated to insert rack slot liquid filling when slide glass sample of the invention needs to heat during processing, without shifting slide glass sample in heat treatment, so that the processing of slide glass sample from start to finish carries out in the same container.When heat treatment, even if generating volatilization, the liquid in gap can be automatically replenished due to capillarity, heat treatment is sufficiently, uniformly, withered phenomenon is not generated, the present invention makes that operating procedure is simpler, reliable, processing specimen amount is bigger and full-automatic operation process convenient to carry out.
Description
Technical field
The invention belongs to biological sample process fields, more particularly to a kind of multichannel slide glass sample with heating function
Incubating device.
Background technique
Immunohistochemistry whole process needs nearly 30 steps, comprising: a) dewaxing, aquation, endogenous enzyme blocking,
It cleans, high temperature hot repair is multiple, cooling, cleaning, primary antibody is incubated for, cleans, secondary antibody is incubated for, clean, synergist is incubated for, cleaning, develops the color, is clear
It washes, serve as a contrast dye, cleaning, dehydration, mounting.By between hospital and hospital technical level and operating habit difference influenced,
It is difficult to standardize.The efficiency and reliability for improving sample disposal becomes the task of top priority.
At present in checkout procedure, reagent pipetting volume sprays sample-adding above thin slice, and load position error is big, and reagent is being sliced
On covering have randomness, it is difficult to ensure whole slice of covering, some samples do not obtain the covering of reagent and false yin occur
Property.It needs to be heated at high temperature during sample disposal, this is to reply antigen or pathogen characteristic protein necessary to original shape
Step.Heating requirements reach 80-100 DEG C or higher temperature and maintain 5-40 minutes.Most common method be pressure cooker boil,
General boiling is boiled or the methods of hot water warm bath.
However, the container or carrier of currently used slide glass sample disposal do not have and can heat or resistance to heated performance.
It needs for slide glass sample to be transferred in heatable container in the midway of slide glass sample disposal and carry out, then need to be transferred to often again
On the station of temperature, the complexity and operating error of operating process are increased.
Realizing that slide glass sample is heated in situ just can simplify slide glass specimen processing operation step and be easier to
In carrying out full-automatic operation process, meet simpler reliable, higher efficiency the requirement of slide glass sample disposal.
Summary of the invention
To solve the above-mentioned problems, a kind of multichannel slide glass sample incubating device with heating function is proposed, it can
The reaction time and operation of the more large batch of slice of processing simultaneously and improvement slice in liquid reagent are to further save the time
And it improves efficiency, and fundamentally ensure the reproducibility and reliability of hot repair process.
A kind of multichannel slide glass sample incubating device with heating function comprising slide glass, cover board, insert rack slot, heating
Device container, support leg, lower discharge outlet, heating plate, electrothermal tube, cover board support, spring leaf, electromagnet, upper water inlet；
The cover board support is intervally arranged by array format to be fixed in insert rack slot；Bullet is fixed on each cover board support
Reed；Sub-assembly made of slide glass and cover board cooperation, is inserted into, the surface of cover board along the gap between cover board support and spring leaf
Spring leaf is squeezed, spring leaf generation spring pressure, which is applied on slide glass and cover board, realizes pressuring action；
Heating tube is embedded in heating plate, and heating plate is mounted in heater canister；Heating tube is connect with external power supply, and
Power and time by the control heating of manual or controller；Heat transfer is to heating plate caused by heating tube；Insert rack trench bottom
Or main body is made of metal；When energization, the heating heat pipe of bottom places the heat for receiving heating plate on hot plate at work
To heat the liquid in insert rack slot.
Further, insert rack slot is provided with discharge outlet, and heater canister is equipped with lower discharge outlet；Upper discharge outlet, lower draining
Mouth carries out drain by manual operation.
Further, insert rack slot is provided with discharge outlet, and heater canister is equipped with lower discharge outlet；Upper discharge outlet, lower draining
Mouth controls drain by electric device (such as solenoid valve).
Further, in insert rack slot it is settable one column perhaps multiple row cover board support to insert a column or multiple row by carrying
Slide glass component made of piece cover board and slide glass are adjacent to forms array type close arrangement.
Further, a biological sample is at least contained above the slide glass.
Further, used material is resistant to higher temperature, is generally possible to tolerance 80oC or more and it is indeformable
Or softening.
Further, described device is provided with the heater of Contiuum type or separate type.
Further, the heating method of the heating plate, using disjunctor or separate type heating rod, heating sheet, electromagnetic heating
Induction system is heated, or perfusion hot liquid carries out warm bath heating into heating container.
Further, when needing to be heat-treated during the slide glass sample disposal, be perfused directly into insert rack slot liquid and
It is heated, without shifting slide glass sample in midway, the processing of slide glass sample is from start to finish carried out in a container.
Further, heater canister is provided with support leg；Heater canister is made of thermal insulation material, plays insulation
Effect.
Compared with the existing technology, the present invention has the following advantages that and technical effect:
The device of the invention enables slide glass sample to place in a manner of erectting or oblique angle is erect rather than in the way of tiling,
It can be arranged with the array type close structure of multiple rows of multiple row；For slide glass component in the case where setting, the liquid in fine gap will not
It drains off, it is ensured that the slide glass sample moment obtains the covering of reagent；Section preparation, which can directly be impregnated or be inserted into hot solution, to be evenly heated,
Even if the liquid in gap generates volatilization during heating also due to capillarity is from the fine gap of slide glass component bottom
Mouth sucking is automatically replenished, and heating sufficiently, does not generate withered phenomenon, ensures the reproducibility and reliability of hot repair process；
All accessories are made using heat proof material in the device of the invention, and be arranged disjunctor or separable heating device, only
Appearance liquid to be perfused into insert rack slot directly to be heated, and make slide glass sample during processing from start to finish in a container
Interior progress.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of multichannel slide glass sample incubating device with heating function of the invention.
Fig. 2 is the diagrammatic cross-section of Fig. 1.
Fig. 3 is the structural schematic diagram of heated base part of the invention.
Fig. 4 is the structural schematic diagram of insert rack slot of the invention.
Fig. 5 is the diagrammatic cross-section of Fig. 4.
Fig. 6 is the structural schematic diagram of slide glass and cover board of the invention.
Fig. 7 a is the front view of slide glass component in Fig. 6；
Fig. 7 b is the A-A sectional view of Fig. 7 a；
Fig. 7 c is the enlarged diagram of the local B of Fig. 7 b.
Fig. 8 is one and setting multiple row slide glass in tank therefor is assigned to assign slot schematic diagram.
In figure: 1- slide glass；2- cover board；3- insert rack slot；4- heater canister；5- support leg；Discharge outlet under 6-；7- heating
Plate；8- electrothermal tube；9- cover board support；10- spring leaf；11- electromagnet；The upper water inlet of 12- and lower discharge outlet.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described, but not limited to this.
As shown in Fig. 1 ~ Fig. 7, a kind of multichannel slide glass sample incubating device with heating function, mainly include slide glass 1,
Cover board 2, insert rack slot 3, heater canister 4, support leg 5, lower discharge outlet 6, heating plate 7, electrothermal tube 8, cover board support 9, spring leaf
10, the components such as electromagnet 11, upper discharge outlet 12.
The cover board support 9 is fixed in insert rack slot 3, and array at a certain distance；It is fixed on each cover board support 9
Spring leaf 10；Sub-assembly made of slide glass 1 and cover board 2 cooperate, is inserted into along the gap between cover board support 9 and spring leaf 10,
The surface rolling spring leaf 10 of cover board 2；Spring leaf 10 is tied using sheet copper, spring steel or other elastic materials and elasticity
It is configured to, generates spring pressure and be applied on slide glass 1 and cover board 2 to compress the two.
Such as Fig. 6, Fig. 7 a ~ Fig. 7 c, slide glass 1 and cover board 2 form one group of slide glass component for being used for sample disposal, and cover board 2 has
Capillary plane forms a capillary gap between slide surface and capillary plane；The channel opening face 601 is connect with capillary plane
And forming angle is 1 ~ 175 degree of plane angle.
Heating tube 8 is embedded in heating plate 7, and heating plate is mounted in heater canister 4；Heating tube 8 and external power supply connect
It connects, and is not drawn into power and the time of control heating by controller；Heat transfer caused by heating tube 8 is to heating plate 7.Insert rack
Slot 3 is made of metal, and is placed in heating plate 7 at work, receives the heat of heating plate 7 to heat the liquid in insert rack slot 3.
Further, insert rack slot 3 is provided with electromagnet 11, when device is in heated condition, controller (not shown) control
Electromagnet 11 processed, which is connected, generates electromagnetic force, heating plate 7 is sucked, heating plate 7 and insert rack slot 3 is made to be in close contact raising heat transfer effect
Rate.
Further, the bottom of insert rack slot 3 is provided with discharge outlet 12, and heater canister 4 is provided with lower discharge outlet 6, on
Discharge outlet 12 and lower discharge outlet 6 are coaxial；Upper discharge outlet 12 is provided with electromagnetism valve block (in figure slightly), can be by controller (in figure slightly) control
The opening and closing of discharge outlet 12 in system.
Further, heater canister 4 is provided with support leg 5；Heater canister 4 is made of thermal insulation material, plays heat preservation
Heat-insulated effect.
Further, a settable column or multiple row cover board support in each slide glass insert rack slot 3, so as to which installation is arranged
One column or multiple row slide glass sample.
Further, used material is resistant to higher temperature, is generally possible to tolerance 80oC or more and it is indeformable
Or fusing.
Further, the heating method of the heating plate 7 similar can also add using electromagnetic heating or with container
It the method for heat function and is heated by the way of hot liquid is perfused.
Further, the controller is using controllers such as PIC single chip microcomputer, 51 single-chip microcontrollers or PLC controllers.
Such as Fig. 8, in insert rack slot a settable column perhaps multiple row cover board support to insert a column or multiple row by slide glass lid
Slide glass component made of plate and slide glass are adjacent to forms array type close arrangement.
According to the announcement and introduction of description above, those skilled in the art in the invention can also be to above embodiment
It changes and modifies.Therefore, the invention is not limited to the specific embodiments disclosed and described above, to of the invention one
A little modifications and changes should also be as falling into the scope of the claims of the present invention.
Claims (10)
1. a kind of multichannel slide glass sample incubating device with heating function, it is characterised in that including slide glass (1), cover board (2),
Insert rack slot (3), heater canister (4), support leg (5), lower discharge outlet (6), heating plate (7), electrothermal tube (8), cover board support
(9), spring leaf (10), electromagnet (11), upper water inlet；
The cover board support (9) is intervally arranged by array format to be fixed in insert rack slot (3)；It is solid on each cover board support (9)
Surely there are spring leaf (10)；Sub-assembly made of slide glass (1) and cover board (2) cooperation, along cover board support (9) and spring leaf (10) it
Between gap insertion, the surface of cover board (2) squeezes spring leaf (10), and spring leaf (10) generates spring pressure and is applied to slide glass (1)
Pressuring action is realized on cover board (2)；
Electrothermal tube (8) is embedded in heating plate (7), and heating plate is mounted in heater canister (4)；Electrothermal tube (8) and external electricity
Source connection, and by the power of manual or controller control heating and time；Heat transfer caused by electrothermal tube (8) is to heating plate
(7)；Insert rack slot (3) bottom or main body are made of metal, and are placed on heating plate (7) at work, are received the heat of heating plate (7)
Amount is to heat the liquid in insert rack slot (3).
2. a kind of multichannel slide glass sample incubating device with heating function according to claim 1, it is characterised in that:
Insert rack slot (3) is provided with discharge outlet (12), and heater canister is equipped with lower discharge outlet (6)；Upper discharge outlet (12), lower discharge outlet
(6) drain is carried out by manual operation.
3. a kind of multichannel slide glass sample incubating device with heating function according to claim 1, it is characterised in that:
Insert rack slot (3) is provided with discharge outlet (12), and heater canister is equipped with lower discharge outlet (6)；Upper discharge outlet (12), lower discharge outlet
(6) drain is controlled by electric device.
4. a kind of multichannel slide glass sample incubating device with heating function according to claim 1, it is characterised in that:
Perhaps multiple row cover board support is pasted to insert a column or multiple row by slide glass cover board and slide glass a settable column in insert rack slot (3)
Slide glass component made of tight forms array type close arrangement.
5. a kind of multichannel slide glass sample incubating device with heating function according to claim 1, it is characterised in that:
The slide glass (1) at least contains a biological sample above.
6. a kind of multichannel slide glass sample incubating device with heating function according to claim 1, it is characterised in that:
Used material is resistant to 80oC or more and it is indeformable or softening.
7. a kind of multichannel slide glass sample incubating device with heating function according to claim 1, it is characterised in that:
Described device is provided with the heater of Contiuum type or separate type.
8. a kind of multichannel slide glass sample incubating device with heating function according to claim 1, it is characterised in that:
The heating method of the heating plate (7), is added using disjunctor or separate type heating rod, heating sheet, electromagnetic heating induction system
Heat, or perfusion hot liquid carries out warm bath heating substitution into heating container.
9. a kind of multichannel slide glass sample incubating device with heating function according to claim 1, it is characterised in that:
When needing to be heat-treated during slide glass sample disposal, liquid is perfused directly into insert rack slot and is heated, without in
Slide glass sample is shifted on way, and the processing of slide glass sample from start to finish carries out in a container.
10. a kind of multichannel slide glass sample incubating device with heating function according to claim 1, feature exist
In: heater canister (4) is provided with support leg (5)；Heater canister (4) is made of thermal insulation material, plays the work of insulation
With.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610160688.9A CN105675383B (en) | 2016-03-21 | 2016-03-21 | A kind of multichannel slide glass sample incubating device with heating function |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610160688.9A CN105675383B (en) | 2016-03-21 | 2016-03-21 | A kind of multichannel slide glass sample incubating device with heating function |
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| CN105675383A CN105675383A (en) | 2016-06-15 |
| CN105675383B true CN105675383B (en) | 2019-06-14 |
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| CN106353516B (en) * | 2016-08-31 | 2019-07-23 | 厦门通灵生物医药科技有限公司 | A kind of device and method of full automatic treatment biology slide sample |
| CN106153436B (en) * | 2016-08-31 | 2021-05-11 | 厦门通灵生物医药科技有限公司 | Heat treatment device for slide specimen detection |
| EP3435063A4 (en) | 2016-03-21 | 2019-11-13 | Xiamen Talent Biomedical Technology Company, Ltd. | Device and method of processing sample slide |
| CN106706379B (en) * | 2016-08-31 | 2023-09-08 | 厦门通灵生物医药科技有限公司 | Device and method for processing slide specimen |
| CN106769382A (en) * | 2016-11-18 | 2017-05-31 | 广州深达生物制品技术有限公司 | The pretreatment unit and method of a kind of slide glass Samples detection |
| CN107144698B (en) * | 2017-06-27 | 2018-06-26 | 珠海意动智能装备有限公司 | Test reaction device, chip-detecting apparatus and its control method |
| CN109421192A (en) * | 2017-08-31 | 2019-03-05 | 无锡拓利普科技有限公司 | Electric boiling plate component and the vulcanizer for using the component |
| CN114354317A (en) * | 2022-01-14 | 2022-04-15 | 厦门通灵生物医药科技有限公司 | Convertible support and detection device thereof |
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Effective date of registration: 20181227 Address after: 361028 Floor 3 and 4 of Building B10, Xiamen Biomedical Industrial Park, 2068 Wengjiao West Road, Haicang District, Xiamen City, Fujian Province Applicant after: Xiamen Tongling biological medicine technology Co., Ltd. Address before: 511458 Guangdong Nansha Guangzhou Information Technology Park software development North 502, Nansha Development Zone Applicant before: ASCEND BIOTECHNOLOGY CO., LTD. |
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