CN107144698B - Test reaction device, chip-detecting apparatus and its control method - Google Patents
Test reaction device, chip-detecting apparatus and its control method Download PDFInfo
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- CN107144698B CN107144698B CN201710501333.6A CN201710501333A CN107144698B CN 107144698 B CN107144698 B CN 107144698B CN 201710501333 A CN201710501333 A CN 201710501333A CN 107144698 B CN107144698 B CN 107144698B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
- G01N2035/00376—Conductive heating, e.g. heated plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00564—Handling or washing solid phase elements, e.g. beads
Abstract
The present invention provides a kind of test reaction device, chip-detecting apparatus and its control method.Test reaction device includes cover, temperature-constant plate and heating unit, temperature-constant plate is provided with liquid tank on the end face towards the cover, reaction chamber is formed between cover and liquid tank, heating unit includes multiple heating plates for abutting against temperature-constant plate bottom surface, and multiple heating plates are arranged along the extending direction of temperature-constant plate.Chip-detecting apparatus passes through main transport mechanism, clean and change liquid mechanism and test reaction device, realize test chip automation movement, and heat preservation, moisturizing having the function of using test reaction device etc., transmission mechanism is allowd to pass through test reaction device by budget speed and preset time, and can realize filling test fluid to test chip by clean and change liquid mechanism and draw test fluid.Multi-angle servicing fluids, imbibition and aeration cleaning can be realized using clean and change liquid mechanism, then realize the detection of biochip efficiently and accurately simultaneously.
Description
Technical field
The present invention relates to a kind of chip detection field more particularly to test reaction device, chip-detecting apparatus and its controls
Method.
Background technology
Biochip technology is by microphotography, according to the intermolecular principle specifically to interact, by life section
Discontinuous analytic process is integrated in the miniature biochemical analysis system on silicon chip or glass-chip surface in field, with reality
Now to accurate, quick, large information capacity the detection of cell, protein, gene and other biological components.According to cured on chip
Biochip can be divided into genetic chip, protein-chip, polysaccharide chip and neuron chip by the difference of biomaterial.
When handling biochip, inspector is needed by a series of operation：Object to be detected is filled first, is then filled miscellaneous
Liquid is handed over, subsequent temperature is reacted within preset temperature, then the reagent of series reaction needed to be reacted again, be finally
Clean surface then can image instrument by CCD and carry out observation analysis by way of centrifuge or air blowing, it is seen then that handle
It is multiple that the equipment that period involves includes multiple devices, the processes such as heat-preserving equipment, manual sample injector, centrifugation apparatus, cleaning equipment, air pressure gun
Miscellaneous degree is high, manual operation efficiency is low, program is cumbersome, is then susceptible to human error, and detection is made to fail.
Invention content
The first object of the present invention is to provide a kind of test reaction device for realizing automation control.
The second object of the present invention is to provide a kind of chip-detecting apparatus for realizing automation reaction treatment.
The third object of the present invention is to provide a kind of control method for the chip-detecting apparatus for realizing automation reaction treatment.
In order to realize the first object of the present invention, the present invention provides a kind of test reaction device, it is characterised in that：Including lid
Body, temperature-constant plate and heating unit, cover cap are closed in the top of temperature-constant plate, and temperature-constant plate is provided with liquid on the end face towards cover
Slot forms reaction chamber between cover and liquid tank, heating unit includes multiple heating plates for abutting against temperature-constant plate bottom surface, multiple
Heating plate is arranged along the extending direction of temperature-constant plate.
Further scheme is that cover is provided with multiple grooves arranged side by side on the end face towards temperature-constant plate, more
A groove is arranged along the extending direction of temperature-constant plate.
Further scheme is that liquid tank is connected by multiple conduits and formed.
Further scheme is that test reaction device further includes water inlet and discharge outlet, water inlet and discharge outlet difference
It is arranged on two ends of liquid tank.
Further scheme is that the distance between slot bottom of discharge outlet and liquid tank is preset height.
Further scheme is that the both sides on the extending direction of temperature-constant plate are provided with sliding slot, and sliding slot transmits for installing
Band component.
In order to realize the second object of the present invention, the present invention provides a kind of chip-detecting apparatus, which is characterized in that including
Transport mechanism, transport mechanism are used to transport test chip,
Clean and change liquid mechanism, clean and change liquid mechanism are used for towards the test chip filling in transport mechanism or draw test
Liquid；
Test reaction device, the test reaction device of test reaction device any one of using the above scheme, transport mechanism
Across reaction chamber.
Further scheme is that transport mechanism includes multiple transfer tape assembles；Multiple transfer tape assembles are in be arranged side by side,
Chip is provided on transfer tape assemble and places position, transfer tape assemble passes through reaction chamber.
Further scheme is that clean and change liquid mechanism includes fluid injection component and imbibition component, the setting of clean and change liquid mechanism
On the end of temperature-constant plate；
Fluid injection component includes the first conduit, and the first conduit is arranged on the top of transport mechanism, and fluid injection component is used for by the
One conduit fills test fluid towards the test chip in transport mechanism；
Imbibition component includes the second conduit, and the second conduit is arranged on the top of transport mechanism, and imbibition component is used for by the
Two conduits draw test fluid towards the test chip in transport mechanism.
In order to realize the third object of the present invention, the present invention provides a kind of control method of chip-detecting apparatus, feature
It is, the chip-detecting apparatus of chip-detecting apparatus any one of using the above scheme；
Control method includes：
Test chip filling test fluid of the clean and change liquid mechanism in transport mechanism；
Transport mechanism drives test chip to pass through test reaction device；
Test chip of the clean and change liquid mechanism in transport mechanism draws test fluid.
The invention has the advantages that by main transport mechanism, clean and change liquid mechanism and test reaction device, realize
Test chip automation movement, and heat preservation, moisturizing having the function of using test reaction device etc. so that transmission mechanism can be with
Pass through test reaction device by budget speed and preset time, and test chip can be realized by clean and change liquid mechanism
It fills test fluid and draws test fluid.Simultaneously multi-angle servicing fluids, imbibition and aeration cleaning can be realized using clean and change liquid mechanism,
Then the detection of biochip efficiently and accurately is realized.
Description of the drawings
Fig. 1 is the structure chart of apparatus for testing chip embodiment of the present invention.
Fig. 2 is structure chart of the apparatus for testing chip embodiment of the present invention under another visual angle.
Fig. 3 is structure chart of the apparatus for testing chip embodiment of the present invention after cover is opened.
Fig. 4 is structure chart of the apparatus for testing chip embodiment of the present invention after cover is opened under another visual angle.
Fig. 5 is the Structure explosion diagram of apparatus for testing chip embodiment of the present invention.
Fig. 6 is the enlarged drawing at A in Fig. 4.
Fig. 7 is sectional view of the apparatus for testing chip embodiment of the present invention at temperature-constant plate.
Fig. 8 is the enlarged drawing at B in Fig. 7.
Fig. 9 is the structure chart of transfer assembly in apparatus for testing chip embodiment of the present invention.
Figure 10 is structure chart of the transfer assembly under another visual angle in apparatus for testing chip embodiment of the present invention.
Figure 11 is the structure chart of the second clean and change liquid mechanism in apparatus for testing chip embodiment of the present invention.
Figure 12 is the structure chart of third clean and change liquid mechanism in apparatus for testing chip embodiment of the present invention.
Figure 13 is structure chart of the third clean and change liquid mechanism under another visual angle in apparatus for testing chip embodiment of the present invention.
Figure 14 is third clean and change liquid mechanism sectional view in apparatus for testing chip embodiment of the present invention.
Figure 15 is the system block diagram of apparatus for testing chip embodiment of the present invention.
Figure 16 is the fundamental diagram of apparatus for testing chip embodiment of the present invention.
Figure 17 is the structure chart of the 4th clean and change liquid mechanism in apparatus for testing chip embodiment of the present invention.
The invention will be further described with reference to the accompanying drawings and embodiments.
Specific embodiment
Referring to figs. 1 to Fig. 4, test chip device includes the cover 12 of shell 11 and mutual cover, is provided in shell 11
Accommodating chamber, the accommodating chamber are used to accommodate the devices such as transport mechanism, clean and change liquid mechanism and test reaction device, and realize automation
Operating plus imbibition or reaction step.Specifically, multiple test fluids are provided on the outer wall of shell 11 and place position, which uses
In placing test fluid storage container 13, chip outlet 111,12 L-shaped setting of cover, cover are provided on 11 left side wall of shell
12 middle part is made using translucent material so that user can supervise the situation of accommodating chamber with permeation parts cover
Control, and form operation notch in the front of shell 11 and conveniently internal unit is operated.
Multigroup pump 14 is provided on the internal perisporium of accommodating chamber, and multiple test fluids are similarly provided in accommodating chamber and are deposited
Storage container 13, pump 14 are used to the test fluid of test fluid storage container 13 being filled into test chip 10 by clean and change liquid mechanism
On, alternatively, pump 14 is used to the test fluid absorption in test chip 10 being discharged into test fluid storage container 13.Certain test fluid
Storage container 13 can both place not used test fluid and be used to store discarded test fluid.
With reference to Fig. 5 and Fig. 6, transmission mechanism includes transfer tape assemble 21, transfer tape assemble 221, transfer tape assemble 231 and passes
Band component 241 is sent, transfer tape assemble 21, transfer tape assemble 221, transfer tape assemble 231 and transfer tape assemble 241 are in abreast to set
It puts, transfer tape assemble 21 is located at outside shell 11, and transfer tape assemble 221, transfer tape assemble 231 and transfer tape assemble 241 are located at
In accommodating chamber.The tablet 211 and the conveyer belt 212 positioned at 211 both sides of tablet that transfer tape assemble 21 includes strip extension, conveyer belt
212 slightly above tablet 211, transfer tape assembles 21 constitute the feeding area of apparatus for testing chip, and multiple test chips 10 can be horizontal
Ground is placed on tablet 211, and drive conveyer belt 212 under the driving of 21 built-in motor of transfer tape assemble, and test chip 10 is passed
It is sent to end.
With reference to Fig. 7 and Fig. 8, also set up that there are three the test reaction device being arranged side by side, three test reactions in accommodating chamber
The structure of device is roughly the same and use principle is identical, therefore is carried out for close to the test reaction device of transfer tape assemble 21 detailed
It describes in detail bright.
Test reaction device includes cover 222, temperature-constant plate 223 and heating unit, towards cover 222 on temperature-constant plate 223
End face on be provided with the liquid tanks of multiple connections, liquid tank is interconnected, including three horizontal strokes extended along its length
The vertical liquid tank extended to liquid tank and width direction, the lid of cover 222 are closed in the top of liquid tank, cover 222 and liquid tank
Between form reaction chamber, reaction chamber is respectively arranged with opening on the extending direction of transfer tape assemble 221.Heating unit packet
Include three heating plates 224 for abutting against 223 bottom surface of temperature-constant plate, the uniform cloth of extending direction of three heating plates 224 along temperature-constant plates 223
It puts.
Test reaction device is also provided with water inlet and discharge outlet 225 on the two of each temperature-constant plate ends, referring to
Fig. 4, temperature-constant plate 223,233,243 are arranged side by side in accommodating chamber, with the temperature-constant plate 233 at middle part for example, discharge outlet 225
The distance between slot bottom of liquid tank is preset height, and the liquid level for preventing liquid is excessively high, and liquid can be discharged from discharge outlet 225
To outside liquid tank, water inlet is then arranged on the other end of discharge outlet 225, is generally used for filling water towards liquid tank.
Both sides on the extending direction of temperature-constant plate are provided with sliding slot, and sliding slot is used to install transfer tape assemble 221, conveyer belt group
Part 221 includes slidable conveyer belt and the limited block on transmission belt, and limited block is used to limit test chip 10,
Chip is formed between the adjacent limited block of homonymy and places position, with reference to Fig. 5, apparatus for testing chip is also according to each transmission belt component
Drive component is equipped with, which includes driving motor 251, transmission belt 252, axis and two groups of roll wheel assemblies 253, roller group
Part includes the idler wheel of two end cooperations, and driving motor 251 drives middle shaft rotation, two roll wheel assemblies 253 by transmission belt 252
Set up separately in two ends of axis, each roll wheel assembly 253 with the transmission band connection of transfer tape assemble 221, utilizes axis respectively
Rotation synchronous drive two conveyer belts synchronously operated in sliding slot, then realize the transport of test chip 10.
Cover 222 is towards being provided with multiple grooves 226 arranged side by side, multiple grooves 226 on the end face of temperature-constant plate 223
Arrange that the extending direction of a groove 226 is perpendicular to the extending direction of temperature-constant plate 223, recessed along the extending direction of temperature-constant plate
Slot 226 is set in tiny channel-shaped, by the setting of multiple grooves 226, can effectively increase end of the cover 222 towards temperature-constant plate
The entire area in face, and water droplet on cover board can effectively be inhibited to be formed, it ensure that experiment effect.
By test reaction device and the matching design of transport mechanism, using multiple separately controllable heating plates to reaction
Chamber, temperature-constant plate and temperature-constant plate are heated, and cause the water thermally equivalent of the conduit of multiple connections, and but also reaction chamber
Interior thermally equivalent, and close the INSULATION DESIGN of temperature-constant plate in cover cap, i.e. the lid of cover 222 closes temperature-constant plate 223, and the lid of cover 232 closes
Temperature-constant plate 233, the lid of cover 242 close temperature-constant plate 243, ensure that each reaction chamber realizes that different temperature accurately controls, and real
The accurate temperature controller in the existing indoor different length region of a reaction chamber.And in addition to temperature controls, the water in temperature-constant plate can by
It after heat, can accelerate to evaporate, humidity can be then provided for reaction chamber, while using the multiple-grooved portion of cover, effectively increase lid
The area of the bottom end face of body can be effectively prevented the formation of water droplet, so as to prevent water droplet from being formed and dropped to from cover
In test chip 10.
Reaction chamber of the cooperation with certain tightness can control the temperature and humidity of reaction chamber well.Again
Person can set one or more temperature sensors and humidity sensor on cover different location, pass through the temperature of sensor
It obtains and humidity obtains, reaction chamber can be controlled in real time.In addition, reaction chamber has entrance at both ends and goes out
Mouthful, the conveyer belt of transport mechanism drives test chip to pass through the reaction chamber, and then test chip can be located in reaction chamber
Test reaction is carried out in preset temperature and default humidity.
With reference to Fig. 9 and Figure 10, and Fig. 4 and Fig. 6 is combined, multiple transfer tape assembles are arranged side by side, and test chip needs
Transfer tape assemble passes through three test reaction devices successively, therefore needs the transfer assembly by being connected between transfer tape assemble
It is shifted, therefore transfer device further includes transfer assembly 31, transfer assembly 32 and transfer assembly 33, transfer assembly in the present embodiment
Structure and application principle all same, between transfer tape assemble 21 and transfer tape assemble 221 chip transmission be to pass through transfer group
Part 31 is come what is shifted, and transfer assembly 32 is connected between transfer tape assemble 221 and transfer tape assemble 231, and transfer assembly 33 connects
Between transfer tape assemble 231 and transfer tape assemble 241.
It is described in detail below with transfer assembly 31, transfer assembly 31 includes housing 311, driving motor 312, driving wheel
314th, driven wheel 319, synchronous belt 315, sliding block 318, main absolute position encoder 313 and from absolute position encoder 316, absolutely
Position coder 313 and individual pen absolute value encoder can be used from absolute position encoder 316, individual pen absolute value encoder can
To be photoelectric encoder, magnetic encoder, resolving encoder, capacitive grating encoder.311 rectangular framework of housing sets and at middle part
Be provided with accommodating chamber, sliding rail 317 be provided on the first side wall of accommodating chamber, sliding rail 317 is set in straight slot, sliding block 318 from
The sliding rail 317 is passed through in accommodating chamber, sliding block 318 is arranged in sliding rail 317 and can be moved along sliding rail 317.312 He of driving motor
It is arranged on from absolute position encoder 316 on the outer wall of the second sidewall of accommodating chamber, driving wheel 314, driven wheel 319 and synchronous belt
315 are arranged in accommodating chamber, and driving wheel 314 is arranged on the output shaft of driving motor 312, main absolute position encoder 318
It is connected on the output shaft of driving motor, synchronous belt 315 is connected between driving wheel 314 and driven wheel 419, and sliding block 318 is set
On synchronous belt 315, it is connect from absolute position encoder 316 by shaft with driven wheel 319.And the diameter of driving wheel 314 with
The diameter and the number of teeth of driven wheel 319 differ, and in the present embodiment, the diameter of driving wheel 314 is less than the diameter of driven wheel 319
Ground is set, and the number of teeth of driving wheel 314 is less than the number of teeth of driven wheel 319.Driving motor can be used servo motor, stepper motor or
DC brushless motor etc..
In order to accurately know the position of test chip 10 and sliding block 318, existing designing scheme generally by
Multi-turn absolute value encoder coordinates driving motor accurately to control turned position.Transfer assembly 31 stirs test chip
When, since the diameter of driving wheel is less than the diameter of driven wheel, therefore driving wheel 314 is obtained by main absolute position encoder 313
Rotational angle data, by obtaining the rotational angle data of driven wheel 319 from absolute position encoder 316, when there is step-out,
Rotation data by comparing driving wheel and driven wheel and the relationship ratio according to two diameters and according to the pass between the number of teeth
System, just can calculate the number of turns and the position for calculating current slide that driven wheel/driving wheel turns over, and the present embodiment uses
Scheme be by two absolute value encoders, different-diameter size, teeth number difference, the driving wheel of the resolution ratio of encoder and driven
Wheel measures calculating, purpose and function at low cost and that this case can be realized using general driving motor.
Then test chip 10 at transfer tape assemble 21 can be sent to transfer tape assemble exactly by sliding block 318
At 221, meanwhile, limited block is collinearly set, and has opening, sliding block 318 between two limited blocks in the width direction of temperature-constant plate
Just by test chip 10 between the limited block that opening enters and is pushed on transmission belt.
On the end of transfer tape assemble 221, i.e., test chip, which enters on the position of transfer tape assemble 221, is provided with first
Clean and change liquid mechanism, the first clean and change liquid mechanism includes fluid injection component 51 in the present embodiment, fluid injection component 51 include L-type support and
Three conduits 511 being arranged on stent, conduit 511 arrange that three conduits 511 are connect respectively with pump 14 in vertical direction, lead to
It crosses under the action of pump 14, test fluid is filled into test chip 10.There are three test position 101, tests for the setting of test chip 10
For placing testing liquid and test fluid, test position 101 sets conduction hole in groove arrangement on adjacent two side for position,
Conduction hole 102 is located on length direction, and conduction hole 103 is located in width direction, in the present embodiment the arc-shaped groove of conduction hole
Setting.During practical application, test fluid is enable to be uniformly distributed in test position 101 during filling test fluid to allow, can passed through
The initial filling position of the cooperation of transfer tape assemble 221 and pump 14, i.e. conduit 511 is located at the opposite side of conduction hole 102, Ran Houchuan
Sending 221 mobile test chip 10 of band component, conduit 511 continues to fill test fluid, as test chip 10 moves, conduit 511 simultaneously
Close to conduction hole 102, the then stopping of conduit 511 filling then realizes that conduit 511 is equably filled on test position 101
Test fluid.Then enter test reaction device under the drive of transfer tape assemble 221, carry out the stop or walking of preset time,
Period, test fluid and prepare liquid were fully reacted also under preset temperature and humidity state.
When test chip 10 is transmitted to the other end by transfer tape assemble 221, then by transfer assembly 32 by test chip
On 10 transfer conveyor belt components 231, the second clean and change liquid mechanism is provided on the end for moving transfer tape assemble 231.Specific ginseng
According to Figure 11, the second clean and change liquid mechanism 52 includes lifting assembly, fluid injection component, imbibition component and chip position, and chip position is used to put
Put test chip 10.Lifting assembly includes driving motor, eccentric wheel, encoder 523, stent 524, sliding block 528 and sliding rail 526,
Encoder is connect with driving motor, and driving motor driving eccentric wheel is circumferentially moved around the output shaft of motor, and sliding block is provided with waist
Circular hole, eccentric wheel coordinate with waist round hole, and sliding block 528 matches with sliding rail 526 and merges and can be slided along sliding rail.
Imbibition component includes three conduits 529, and three conduits 529 are connected with pump, are used for from the side of test chip 10
Conduction hole draw test fluid, three conduits 529 are arranged on 527 on sliding block.Lifting assembly connect with imbibition component and can drive
Imbibition component relative to chip position lifting moving, realize when chip in place after to test chip progress imbibition processing.
Fluid injection component includes 521, three conduits 522 of stent and three conduits 528, stent 521 are fixedly provided in shell
In 11, three conduits 522 are connected with pump, and in the setting of filling inclination angle between conduit 522 and horizontal plane, inclination angle is at an acute angle sets for filling
It puts, the outlet of conduit 522 is located relative on the side of conduction hole 103, i.e. the entrance position of the outlet of conduit 522 and conduit 529
In on the both sides of test position 101.
Three conduits 528 are arranged on sliding block 528, and three conduits 528 are also to be moved, conduit with the movement of sliding block 528
528 position is correspondingly set with the conduction hole 102 of test chip, and three conduits 528 are used to fill towards test chip 10
Test fluid
Second clean and change liquid mechanism 52 further includes the separate sheet 525 for being arranged on 529 both sides of conduit, and separate sheet 525 is in strip
Shape is set, and separate sheet 525 is provided with inclined guide surface on the end close to stent 521, and two separate sheets 525 are located at test
The top of chip and to the position-limiting action on test chip 10 its vertical direction, the reason is that conduit 528 is when drawing test fluid,
Suction may suck up test chip 10, therefore effectively test chip can be limited by separate sheet 525, ensure
Conduit 528 is efficiently separated with test chip.
When second clean and change liquid mechanism 52 is worked, in the end of transfer tape assemble 231, test chip 10 is for the first time
When entering the chip position of clean and change liquid mechanism, test chip 10 is to be mounted with test fluid, the second clean and change liquid mechanism 52 into
Row needs first to carry out cleaning step when replacing second of test fluid.
Therefore the control method of the second clean and change liquid mechanism 52 includes：
Test fluid in test chip 10 is drawn by conduit 529；
Then, cleaning solution is filled by conduit 522, meanwhile, conduit 529 draws cleaning solution simultaneously, due to conduit 522 and leads
Pipe 529 is on both sides, thus inclined direction fill cleaning solution, and convection current is generated on the test position of test chip, then have
Effect improves cleaning force.
Then, conduit 522 stops filling cleaning solution, and conduit 529 draws cleaning solution clean；
Finally, second of test fluid is filled to test chip by conduit 527, during filling, test chip 10 is by conveyer belt
Component 231 drives, and with the movement of test chip 10, conduit 527 fills.
As test chip by transfer tape assemble 231 drives test reaction device into temperature-constant plate 233, by it is default when
Between, the reaction of temperature and humidity, test chip is sent to the other end, then again by transfer assembly 32, by test chip
Transfer tape assemble 241 is transferred to from transfer tape assemble 231.The starting end of transfer tape assemble 241 is provided with the second cleaning to change
Liquid mechanism 52, subsequent second clean and change liquid mechanism 52 perform test chip above-mentioned control method, and second of test fluid is inhaled
It takes, and after cleaning, adds the third test fluid.
Test chip is driven by transfer tape assemble 241 again, into the test reaction device of temperature-constant plate 243, by default
Time, the reaction of temperature and humidity, test chip are sent to the other end, finally by third clean and change liquid mechanism 53.
With reference to Figure 12 to Figure 14, clean and change liquid mechanism 53 include lifting assembly, translation component, air blowing component, fluid injection component,
Imbibition component and chip position, translation component include driving motor 531, stent 532, eccentric wheel 539, sliding block 534 and sliding rail 533,
The output shaft of driving motor 531 is connect with eccentric wheel 539, and eccentric wheel 539 deviates the axis setting of output shaft, driving motor
531 driving eccentric wheels 539 are circumferentially moved around output shaft, and sliding block 534 is provided with waist round hole 538, eccentric wheel 539 and waist round hole
538 cooperations, in arcuation setting, sliding rail 534 and driving motor 531 be fixedly connected on stent 532 stent 532, and sliding rail 533 is along water
Square extend to the direction of travel of arrangement and test chip, sliding block 534 and sliding rail 533 are with merging and can be slided along sliding rail 533, air blowing
Component is arranged on sliding block 534.
Air blowing component includes gas passage and the intake interface 536 of compressed air, and air pump interface 536 and gas passage connect
It connects, gas passage is surrounded by pipeline block, and the gas passage of connection, the first end connection air inlet connecting of gas passage are opened up in pipeline block
Mouthfuls 536, the second end setting of gas passage there are three gas outlet 536, gas outlet 536 towards conduit 545 entrance tilt and set
It puts, the gas outlet 536 of gas passage is blown for the chip surface on chip position.
Lifting assembly includes driving motor 523, stent 524, eccentric wheel, sliding block 527 and sliding rail 526, driving motor driving
Eccentric wheel is circumferentially moved around the output shaft of motor, and sliding block is provided with waist round hole, and eccentric wheel coordinates with waist round hole, 527 He of sliding rail
Driving motor 523 is fixed on stent 524 and is arranged in a vertical direction, and sliding block 544 and sliding rail 543 are with merge can be along sliding rail
543 slide, and imbibition component is arranged on sliding block 544.
Imbibition component includes three conduits 545, the entrance of conduit 545 set towards chip position and be used to drawing test fluid with
Cleaning solution, three conduits 545 simultaneously can accordingly move down into drawing for test chip 10 with the lifting moving of sliding block 544, entrance
In discharge orifice 102.The outlet of conduit 546 and the entrance of conduit 545 are arranged on the rear side of the extending direction of sliding rail 533.
Third clean and change liquid mechanism 53 further includes the separate sheet 537 for being arranged on 545 both sides of conduit, separate sheet and positioned at lifting
Between component and chip position, separate sheet 537 is set in strip, and separate sheet 537 is provided on the end close to stent 532 inclines
Oblique guide surface, two separate sheets 537 are located at the top of test chip and the limiting on test chip 10 its vertical direction are made
With the reason is that conduit 545, when drawing test fluid, suction may suck up test chip 10, therefore pass through separate sheet 537
Effectively test chip can be limited.
Fluid injection component includes three conduits 546, and three conduits 546 are arranged on the position between two separate sheets 537, and three
It is set in parallel, can also be set certainly with the filling inclination angle of angle at an acute angle, three conduits between a conduit 546 and horizontal plane
546 are used to fill cleaning solution towards chip position.
Third clean and change liquid mechanism 53 changes liquid processing step, control method packet in the last cleaning of processing test chip
It includes：
Test chip 10 is sent to the chip position of third clean and change liquid mechanism 53；
Lifting assembly drops to conduit 545 and cooperatively on position, conduit 545 is inhaled in the conduction hole of test chip 10
Take the test fluid in test chip 10；
Cleaning is then carried out, conduit 546 fills cleaning solution to test chip 10, while conduit 545 is also simultaneously
Cleaning solution is drawn；
Then conduit 546 stops filling cleaning solution；
Finally, translation Component driver air blowing component is moved towards 545 ground of conduit, and air blowing component is towards chip while mobile
Position is blown, meanwhile, conduit 545 draws the remaining cleaning solution in test chip 10, finally all inhales the cleaning solution on chip position
It takes clean.
With reference to Figure 15 and Figure 16, system block diagram and operation principles figure below in conjunction with apparatus for testing chip carry out test stream
Journey explanation.Apparatus for testing chip is usually to be used cooperatively with external computer or embedded system or single-chip computer control system,
And the processing module with processing operational capability can be integrated in apparatus for testing chip, the control in apparatus for testing chip
Panel or upper computer software such as computer, tablet, mobile phone etc. can be configured parameters, the parameter of setting include temperature,
Time, humidity, transmission speed etc., additionally it is possible to pass through control panel or the operating state of apparatus for testing chip.
Apparatus for testing chip includes multiple driving units, multiple driving motors and multiple encoders, such as above-described embodiment institute
It is mentioned to the function to be realized and effect, multiple driving motors are respectively used to driving transfer tape assemble, transfer assembly, for adding
The pump of note and the pump for absorption, a four road driving motor of driving unit band and encoder, driving unit are connected using Ethernet
The driving plate connect, computer are connect by communication unit with each driving unit, and the mode that series connection can be used in connection mode connects,
Computer can efficiently be driven each driving motor by coding, at the same using communication unit and humidity sensor,
Temperature sensor and heating unit are controlled or the acquisition of information
When apparatus for testing chip handles test chip to be tested, control method includes：
First, it fills on object to be detected to test chip 10, is subsequently placed on the transfer tape assemble 21 of feeding area；
Then, test chip is sent to end, and by the transfer of transfer assembly 31, test chip is transferred to conveyer belt group
On the end of part 221；
Then, fluid injection component 51 fills test fluid to test chip；
Then, transfer tape assemble 221 transfers test chip, and by the test reaction device of temperature-constant plate 223, and
It is reacted 20 minutes to 120 minutes between 25 DEG C to 85 DEG C；
Then, subsequent test chip is sent to the other end, and passes through transfer assembly 32 and be transferred on transfer tape assemble 231
End on；
Then, the second clean and change liquid mechanism 52 performs test chip above-mentioned processing step, including drawing test fluid, leads to
Over cleaning liquid cleans, and adds second of test fluid；
Then, transfer tape assemble 231 transfers test chip, and by the test reaction device of temperature-constant plate 233, and
It is reacted 20 minutes to 120 minutes between 25 DEG C to 85 DEG C；
Then, subsequent test chip is sent to the other end, and passes through transfer assembly 33 and be transferred on transfer tape assemble 241
End on；
Then, above-mentioned processing step is being performed to test chip by the second clean and change liquid mechanism 52, including drawing the
Two test fluids, are cleaned by cleaning solution, add the third test fluid；
Then, transfer tape assemble 241 transfers test chip, and by the test reaction device of temperature-constant plate 243, and
It is reacted 20 minutes to 120 minutes between 25 DEG C to 85 DEG C；
Finally, above-mentioned processing step performs test chip by third clean and change liquid mechanism 53, develops the color including drawing
Liquid third test fluid, is cleaned by cleaning solution, and blowing is blotted, finally from 111 output of chip outlet.It is detected by the chip of this case
Device is instead of artificial detection, it can be achieved that continual continuous detection, greatly improves detection efficiency, avoids the occurrence of detection mistake
It loses.
It is further to note that this front side, rear side and left and right sides in embodiment is referred to along transfer tape assemble
Direction of travel, the direction of advance of transfer tape assemble is front side, and the opposite side of front side is rear side, therefore for the second clean and change liquid
The conduit 528 of mechanism 52 is to be located at front side, and conduit 522 is set positioned at rear side and by left side, and conduit 529 is to be located at right side；
Conduit 545 and conduit 546 in second clean and change liquid mechanism 52 are to be located at rear side, and air blowing component is then to be located at front side.
With reference to Figure 17, the first clean and change liquid mechanism 51 in above-described embodiment and the second clean and change liquid mechanism 52 is replaceable makes
Test chip 20 is used with the test chip 10 in the 4th clean and change liquid mechanism 54 and above-described embodiment is replaceable.
Specifically, the second clean and change liquid mechanism 54 includes lifting assembly, fluid injection component, imbibition component and chip position, chip
Position is used to place test chip 20.There are three test positions 201 for the setting of test chip 20, test position for placing testing liquid and survey
Test solution, test position 201 set conduction hole 202,203 in groove arrangement on opposite two side, drain in the present embodiment
The arc-shaped groove setting in hole.
Lifting assembly includes driving motor, eccentric wheel, encoder 543, stent 544, sliding block 548 and sliding rail 546, encoder
It is connect with driving motor, driving motor driving eccentric wheel is circumferentially moved around the output shaft of motor, and sliding block is provided with waist round hole, partially
Heart wheel coordinates with waist round hole, and sliding block 548 matches with sliding rail 546 and merges and can be slided along sliding rail.
Imbibition component includes three conduits 549, and three conduits 549 are connected with pump, are used for the drainage from test chip 20
Draw test fluid in hole.Fluid injection component includes three conduits 541 vertically arranged side by side and the conduit 542 set laterally side by side, and three
A conduit 549 and three conduits 541 connect with pump and fill test fluid, three conduits 549 and three towards test chip 20 respectively
A conduit 541 is arranged on sliding block 548, also, the outlet of the entrance of conduit 549 and conduit 541 is used for towards the test core
The middle part of piece, the outlet of conduit 542 is for towards the side of test chip, and conduit 542 is inclined or with being parallel to horizontal plane
Setting.The position of conduit 549 is correspondingly set with the conduction hole 202,203 of test chip, and lifting assembly is used to drive conduit
549 and the lifting moving of conduit 541, realize when chip in place after imbibition processing and filling processing are carried out to test chip.
When 4th clean and change liquid mechanism 54 is worked, the end in transfer tape assemble, test chip 20 is entering
During the chip position of clean and change liquid mechanism, discriminably carry out drawing the first test fluid, the second test fluid of filling, draw the second test
Liquid and filling third test fluid, then in test reaction device is entered.
Certain third clean and change liquid mechanism 53 is also what can be replaced, can be by the 4th clean and change liquid mechanism 54 with blowing
It is replaced after component combination, function and effect are identical and can achieve the object of the present invention.
By main transport mechanism, clean and change liquid mechanism and test reaction device, test chip automatic moving is realized
It is dynamic, and heat preservation, moisturizing having the function of using test reaction device etc. so that transmission mechanism can by budget speed and it is default when
Between by test reaction device, and filling test fluid can be realized to test chip by clean and change liquid mechanism and is drawn and surveyed
Test solution.Multi-angle servicing fluids, imbibition and aeration cleaning can be realized using clean and change liquid mechanism, then realize that test chip is high simultaneously
The accurate detection of effect.
Above-described embodiment is the preferred embodiment of this case, has a variety of variations, such as above-mentioned reality in practical application
It applies in example and is accurately controlled using driving motor, general motor cooperation position sensor naturally also may be used
Realize accurate transmission and positioning, and for transfer assembly, it, can also general electricity other than the transfer assembly in such as above-described embodiment
Machine also can be achieved on test chip the transfer between different transmission belt components with movable slider.
In addition, the walking path of the test chip of above-described embodiment is set with spiraling or in square-wave-shaped sets in wriggling
It puts, is the volume that can efficiently reduce apparatus for testing chip, can naturally also set along a straight line, this is just no longer needed
Want transfer assembly, it is only necessary to addition clean and change liquid mechanism in place and test reaction device.
Claims (14)
1. test reaction device, it is characterised in that：Including cover, temperature-constant plate and heating unit, the cover cap is closed in the perseverance
The top of warm plate, the temperature-constant plate are provided with liquid tank, the cover and the liquid tank on the end face towards the cover
Between form reaction chamber, the heating unit includes multiple heating plates for abutting against the temperature-constant plate bottom surface, it is multiple described plus
Backing is arranged along the extending direction of the temperature-constant plate；
The cover is towards being provided with multiple grooves arranged side by side, multiple equal edges of groove on the end face of the temperature-constant plate
The extending direction arrangement of the temperature-constant plate.
2. test reaction device according to claim 1, it is characterised in that：
The liquid tank is connected by multiple conduits and is formed.
3. test reaction device according to claim 1, it is characterised in that：
The test reaction device further includes water inlet and discharge outlet, and the water inlet and the discharge outlet are separately positioned on described
On two ends of liquid tank.
4. test reaction device according to claim 3, it is characterised in that：
The distance between slot bottom of the discharge outlet and the liquid tank is preset height.
5. test reaction device according to any one of claims 1 to 4, it is characterised in that：
Both sides on the extending direction of the temperature-constant plate are provided with sliding slot, and the sliding slot is used to install transfer tape assemble.
6. chip-detecting apparatus, which is characterized in that including
Transport mechanism, the transport mechanism include transfer tape assemble, and the transfer tape assemble is used to transport test chip；
Clean and change liquid mechanism, the clean and change liquid mechanism are used for towards the test chip filling in the transport mechanism or draw survey
Test solution；
Test reaction device includes cover, temperature-constant plate and heating unit, and the cover cap is closed in the top of the temperature-constant plate, described
Temperature-constant plate is provided with liquid tank on the end face towards the cover, and reaction chamber is formed between the cover and the liquid tank
Room, the heating unit include multiple heating plates for abutting against the temperature-constant plate bottom surface, and multiple heating plates are along the constant temperature
The extending direction arrangement of plate；
The transfer tape assemble passes through the reaction chamber.
7. chip-detecting apparatus according to claim 6, it is characterised in that：
The cover is towards being provided with multiple grooves arranged side by side, multiple equal edges of groove on the end face of the temperature-constant plate
The extending direction arrangement of the temperature-constant plate.
8. chip-detecting apparatus according to claim 6, it is characterised in that：
The liquid tank is connected by multiple conduits and is formed.
9. chip-detecting apparatus according to claim 6, it is characterised in that：
The test reaction device further includes water inlet and discharge outlet, and the water inlet and the discharge outlet are separately positioned on described
On two ends of liquid tank.
10. chip-detecting apparatus according to claim 9, it is characterised in that：
The distance between slot bottom of the discharge outlet and the liquid tank is preset height.
11. according to claim 6 to 10 any one of them chip-detecting apparatus, it is characterised in that：
Both sides on the extending direction of the temperature-constant plate are provided with sliding slot, and the sliding slot is used to install transfer tape assemble.
12. chip-detecting apparatus according to claim 6, it is characterised in that：
The transport mechanism includes multiple transfer tape assembles；
Multiple transfer tape assembles are provided with chip and place position, the conveyer belt in being arranged side by side on the transfer tape assemble
Component passes through the reaction chamber.
13. chip-detecting apparatus according to claim 6, it is characterised in that：
The clean and change liquid mechanism includes fluid injection component and imbibition component, and the clean and change liquid mechanism is arranged on the temperature-constant plate
On end；
The fluid injection component includes the first conduit, and first conduit is arranged on the top of the transport mechanism, the fluid injection group
Part is used to fill test fluid towards the test chip in the transport mechanism by first conduit；
The imbibition component includes the second conduit, and second conduit is arranged on the top of the transport mechanism, the imbibition group
Part is used to draw test fluid towards the test chip in the transport mechanism by second conduit.
14. the control method of chip-detecting apparatus, which is characterized in that the chip-detecting apparatus using the claims 6 to
13 any one of them chip-detecting apparatus；
The control method includes：
Test chip filling test fluid of the clean and change liquid mechanism in the transport mechanism；
The transport mechanism drives the test chip to pass through the test reaction device；
Test chip of the clean and change liquid mechanism in the transport mechanism draws test fluid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710501333.6A CN107144698B (en) | 2017-06-27 | 2017-06-27 | Test reaction device, chip-detecting apparatus and its control method |
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| CN201710501333.6A CN107144698B (en) | 2017-06-27 | 2017-06-27 | Test reaction device, chip-detecting apparatus and its control method |
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| CN107144698B true CN107144698B (en) | 2018-06-26 |
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| US4384193A (en) * | 1981-06-09 | 1983-05-17 | Immulok, Inc. | Incubating device for specimen mounted on glass slides in immunoassays |
| CN201886369U (en) * | 2010-11-29 | 2011-06-29 | 李江超 | Multifunctional pathology glass slide temperature control instrument |
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