CN107144698A - Test reaction device, chip-detecting apparatus and its control method - Google Patents
Test reaction device, chip-detecting apparatus and its control method Download PDFInfo
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- CN107144698A CN107144698A CN201710501333.6A CN201710501333A CN107144698A CN 107144698 A CN107144698 A CN 107144698A CN 201710501333 A CN201710501333 A CN 201710501333A CN 107144698 A CN107144698 A CN 107144698A
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- test
- chip
- reaction device
- temperature
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
- G01N2035/00376—Conductive heating, e.g. heated plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00564—Handling or washing solid phase elements, e.g. beads
Abstract
The present invention provides a kind of test reaction device, chip-detecting apparatus and its control method.Test reaction device includes lid, temperature-constant plate and heater, temperature-constant plate is provided with liquid tank on the end face towards the lid, reaction chamber is formed between lid and liquid tank, heater includes multiple heating plates for abutting against temperature-constant plate bottom surface, bearing of trend arrangement of multiple heating plates along temperature-constant plate.Chip-detecting apparatus passes through main transport mechanism, clean and change liquid mechanism and test reaction device, realize test chip automation movement, and the function such as insulation, the moisturizing having using test reaction device, allow transmission mechanism to pass through test reaction device by budget speed and preset time, and filling test fluid can be realized to test chip by clean and change liquid mechanism and draw test fluid.Multi-angle servicing fluids, imbibition and aeration cleaning can be realized using clean and change liquid mechanism simultaneously, then realizes that biochip efficiently and accurately is detected.
Description
Technical field
The present invention relates to chip detection field, more particularly to a kind of test reaction device, chip-detecting apparatus and its control
Method.
Background technology
Biochip technology is by microphotography, according to the intermolecular principle specifically interacted, by life section
Discontinuous analysis process integration is in the miniature biochemical analysis system on silicon or glass-chip surface in field, with reality
Now to accurate, quick, large information capacity the detection of cell, protein, gene and other biological components.According to what is solidified on chip
Biochip, can be divided into genetic chip, protein-chip, many carbohydrate chips and neuron chip by the difference of biomaterial.
When handling biochip, inspector is needed by a series of operation：Thing to be detected is filled first, is then filled miscellaneous
Liquid is handed over, subsequent temperature is reacted within preset temperature, then needs the reagent of series reaction to be reacted again, be finally
Clean surface then can image instrument by CCD and carry out observation analysis by way of centrifuge or air blowing, it is seen then that in processing
The equipment that period involves includes multiple devices such as heat-preserving equipment, manual sample injector, centrifugation apparatus, cleaning equipment, air pressure gun, and process is multiple
Miscellaneous degree is high, artificial operating efficiency is low, program is cumbersome, human error then easily occurs, detection is failed.
The content of the invention
The first object of the present invention is to provide a kind of test reaction device for realizing Automated condtrol.
The second object of the present invention is to provide a kind of chip-detecting apparatus for realizing automation reaction treatment.
The third object of the present invention is to provide a kind of control method for the chip-detecting apparatus for realizing automation reaction treatment.
In order to realize the first object of the present invention, the present invention provides a kind of test reaction device, it is characterised in that：Including lid
Body, temperature-constant plate and heater, lid are covered in the top of temperature-constant plate, and temperature-constant plate is provided with liquid on the end face towards lid
Groove, forms reaction chamber, heater includes multiple heating plates for abutting against temperature-constant plate bottom surface, multiple between lid and liquid tank
Bearing of trend arrangement of the heating plate along temperature-constant plate.
Further scheme is that lid is provided with multiple grooves being arranged side by side on the end face towards temperature-constant plate, many
Individual groove is arranged along the bearing of trend of temperature-constant plate.
Further scheme is that liquid tank is connected by multiple conduits and formed.
Further scheme is that test reaction device also includes water inlet and discharge outlet, water inlet and discharge outlet difference
It is arranged on two ends of liquid tank.
Further scheme is that the distance between bottom land of discharge outlet and liquid tank is preset height.
Further scheme is that the both sides on the bearing of trend of temperature-constant plate are provided with chute, and chute, which is used to install, to be transmitted
Band component.
In order to realize the second object of the present invention, the present invention provides a kind of chip-detecting apparatus, it is characterised in that including
Transport mechanism, transport mechanism is used to transport test chip,
Clean and change liquid mechanism, clean and change liquid mechanism is used for towards the test chip filling in transport mechanism or draws test fluid；
Test reaction device, test reaction device uses the test reaction device of any one of such scheme, and transport mechanism is passed through
Reaction chamber.
Further scheme is that transport mechanism includes multiple transfer tape assembles；Multiple transfer tape assembles are in be arranged side by side,
Chip is provided with transfer tape assemble and places position, transfer tape assemble passes through reaction chamber.
Further scheme is that clean and change liquid mechanism includes fluid injection component and imbibition component, and clean and change liquid mechanism is set
On the end of temperature-constant plate；
Fluid injection component includes the first conduit, and the first conduit is arranged on the top of transport mechanism, and fluid injection component is used to lead by first
Pipe fills test fluid towards the test chip in transport mechanism；
Imbibition component includes the second conduit, and the second conduit is arranged on the top of transport mechanism, and imbibition component is used to lead by second
Pipe draws test fluid towards the test chip in transport mechanism.
In order to realize the third object of the present invention, the present invention provides a kind of control method of chip-detecting apparatus, its feature
It is, chip-detecting apparatus uses the chip-detecting apparatus of any one of such scheme；
Control method includes：
Clean and change liquid mechanism fills test fluid to the test chip in transport mechanism；
Transport mechanism drives test chip to pass through test reaction device；
Clean and change liquid mechanism draws test fluid to the test chip in transport mechanism.
The beneficial effects of the invention are as follows by main transport mechanism, clean and change liquid mechanism and test reaction device, realize
Test chip automation movement, and the function such as the insulation, the moisturizing that have using test reaction device so that transmission mechanism can be with
Pass through test reaction device by budget speed and preset time, and test chip can be realized by clean and change liquid mechanism
Fill test fluid and draw test fluid.Multi-angle servicing fluids, imbibition and aeration cleaning can be realized using clean and change liquid mechanism simultaneously,
Then realize that biochip efficiently and accurately is detected.
Brief description of the drawings
Fig. 1 is the structure chart of apparatus for testing chip embodiment of the present invention.
Fig. 2 is structure chart of the apparatus for testing chip embodiment of the present invention under another visual angle.
Fig. 3 is structure chart of the apparatus for testing chip embodiment of the present invention after lid is opened.
Fig. 4 is structure chart of the apparatus for testing chip embodiment of the present invention after lid is opened under another visual angle.
Fig. 5 is the Structure explosion diagram of apparatus for testing chip embodiment of the present invention.
Fig. 6 is the enlarged drawing at A in Fig. 4.
Fig. 7 is sectional view of the apparatus for testing chip embodiment of the present invention at temperature-constant plate.
Fig. 8 is the enlarged drawing at B in Fig. 7.
Fig. 9 is the structure chart of transfer assembly in apparatus for testing chip embodiment of the present invention.
Figure 10 is structure chart of the transfer assembly under another visual angle in apparatus for testing chip embodiment of the present invention.
Figure 11 is the structure chart of the second clean and change liquid mechanism in apparatus for testing chip embodiment of the present invention.
Figure 12 is the structure chart of the 3rd clean and change liquid mechanism in apparatus for testing chip embodiment of the present invention.
Figure 13 is structure chart of the 3rd clean and change liquid mechanism under another visual angle in apparatus for testing chip embodiment of the present invention.
Figure 14 is the 3rd clean and change liquid mechanism sectional view in apparatus for testing chip embodiment of the present invention.
Figure 15 is the system block diagram of apparatus for testing chip embodiment of the present invention.
Figure 16 is the fundamental diagram of apparatus for testing chip embodiment of the present invention.
Figure 17 is the structure chart of the 4th clean and change liquid mechanism in apparatus for testing chip embodiment of the present invention.
Below in conjunction with drawings and Examples, the invention will be further described.
Embodiment
Referring to figs. 1 to Fig. 4, test chip device includes being provided with the lid 12 of shell 11 and mutual lid, shell 11
Accommodating chamber, the accommodating chamber is used to accommodate the devices such as transport mechanism, clean and change liquid mechanism and test reaction device, and realizes automation
Operating plus imbibition or reactions steps.Specifically, multiple test fluids are provided with the outer wall of shell 11 and place position, placement position is used
In placing test fluid storage container 13, chip outlet 111 is provided with the left side wall of shell 11, lid 12 is L-shaped to be set, lid
12 middle part is made using light transmissive material so that user can be supervised with permeation parts lid to the situation of accommodating chamber
Control, and form operation breach in the front of shell 11 and conveniently internal unit is operated.
Multigroup pump 14 is provided with the internal perisporium of accommodating chamber, and multiple test fluids are similarly provided with accommodating chamber and are deposited
Storage container 13, pump 14 is used to the test fluid of test fluid storage container 13 being filled into test chip 10 by clean and change liquid mechanism
On, or, pump 14, which is used to draw the test fluid in test chip 10, to be discharged into test fluid storage container 13.Certain test fluid
Storage container 13 can both place untapped test fluid, be also for depositing discarded test fluid.
Reference picture 5 and Fig. 6, transmission mechanism include transfer tape assemble 21, transfer tape assemble 221, transfer tape assemble 231 and passed
Band component 241 is sent, transfer tape assemble 21, transfer tape assemble 221, transfer tape assemble 231 and transfer tape assemble 241 are in abreast to set
Put, transfer tape assemble 21 is located at outside shell 11, and transfer tape assemble 221, transfer tape assemble 231 and transfer tape assemble 241 are located at
In accommodating chamber.Transfer tape assemble 21 includes flat board 211 and the conveyer belt 212 positioned at the both sides of flat board 211 that strip extends, conveyer belt
212 are slightly above flat board 211, and transfer tape assemble 21 constitutes the feeding area of apparatus for testing chip, and multiple test chips 10 can be horizontal
Ground is placed on flat board 211, and drive conveyer belt 212 under the driving of the built-in motor of transfer tape assemble 21, and test chip 10 is passed
It is sent to end.
Three test reaction being arranged side by side devices, three test reactions are additionally provided with reference picture 7 and Fig. 8, accommodating chamber
The structure of device is roughly the same and use principle is identical, therefore is carried out exemplified by close to the test reaction device of transfer tape assemble 21 detailed
Describe in detail bright.
Test reaction device includes lid 222, temperature-constant plate 223 and heater, towards lid 222 on temperature-constant plate 223
End face on be provided with the liquid tanks of multiple connections, liquid tank is interconnected, including three horizontal strokes extended along its length
The vertical liquid tank extended to liquid tank and width, lid 222 is covered in the top of liquid tank, lid 222 and liquid tank
Between form reaction chamber, reaction chamber is respectively arranged with opening on the bearing of trend of transfer tape assemble 221.Heater bag
Include three heating plates 224 for abutting against the bottom surface of temperature-constant plate 223, bearing of trend uniform cloth of three heating plates 224 along temperature-constant plate 223
Put.
Test reaction device is also provided with water inlet and discharge outlet 225 on the two of each temperature-constant plate ends, referring to
Fig. 4, temperature-constant plate 223,233,243 is arranged side by side in accommodating chamber, with the temperature-constant plate 233 at middle part for example, discharge outlet 225
It is preset height with the distance between the bottom land of liquid tank, prevents that the liquid level of liquid is too high, liquid can be discharged from discharge outlet 225
To outside liquid tank, water inlet is then arranged on the other end of discharge outlet 225, and it is generally used for filling water towards liquid tank.
Both sides on the bearing of trend of temperature-constant plate are provided with chute, and chute is used to install transfer tape assemble 221, conveyer belt group
Part 221 includes slidable conveyer belt and the limited block on transmission belt, and limited block is spacing for being carried out to test chip 10,
Formation chip placement position, reference picture 5 between the adjacent limited block of homonymy, apparatus for testing chip is always according to each transmission belt component
Drive component is equipped with, the drive component includes motor 251, transmission belt 252, axis and two groups of roll wheel assemblies 253, roller group
Part includes the roller that two ends coordinate, and motor 251 drives middle shaft rotation, two roll wheel assemblies 253 by transmission belt 252
Set up separately in two ends of axis, conveyer belt of each roll wheel assembly 253 respectively with transfer tape assemble 221 is connected, and utilizes axis
Rotation it is synchronous drive synchronously being operated in chute for two conveyer belts, then realize the transport of test chip 10.
Lid 222 is towards being provided with multiple grooves 226 being arranged side by side, multiple grooves 226 on the end face of temperature-constant plate 223
Arrange that the bearing of trend of a groove 226 is perpendicular to the bearing of trend of temperature-constant plate 223, recessed along the bearing of trend of temperature-constant plate
Groove 226 is set in tiny channel-shaped, by the setting of multiple grooves 226, can effectively increase end of the lid 222 towards temperature-constant plate
The entire area in face, and can effectively suppress water droplet on cover plate and formed, it is ensured that experiment effect.
By test reaction device and the matching design of transport mechanism, using multiple separately controllable heating plates to reaction
Chamber, temperature-constant plate and temperature-constant plate are heated, and cause the water thermally equivalent of the conduit of multiple connections, and also cause reaction chamber
Interior thermally equivalent, and cover the INSULATION DESIGN of temperature-constant plate, i.e. lid 222 in lid and cover temperature-constant plate 223, lid 232 is covered
Temperature-constant plate 233, lid 242 covers temperature-constant plate 243, it is ensured that each reaction chamber realizes that different temperature are accurately controlled, and in fact
The accurate temperature controller in the different length region in an existing reaction chamber.And except temperature control, water in temperature-constant plate can by
After heat, evaporation can be accelerated, then humidity can be provided for reaction chamber, while using many groove portions of lid, effectively increase is covered
The area of the bottom end face of body, can effectively prevent the formation of water droplet, so as to prevent water droplet from being formed and dropped to from lid
In test chip 10.
Coordinate the reaction chamber with certain tightness, the temperature and humidity of reaction chamber can be controlled well.Again
Person, one or more temperature sensors and humidity sensor can be set on lid diverse location, passes through the temperature of sensor
Obtain and humidity is obtained, reaction chamber can be in real time controlled.In addition, reaction chamber has entrance at two ends and gone out
Mouthful, the conveyer belt of transport mechanism drives test chip to pass through the reaction chamber, and then test chip can be located in reaction chamber
Test reaction is carried out in preset temperature and default humidity.
Reference picture 9 and Figure 10, and Fig. 4 and Fig. 6 is combined, multiple transfer tape assembles are to be arranged side by side, and test chip needs
Transfer tape assemble sequentially passes through three test reaction devices, therefore needs the transfer assembly by being connected between transfer tape assemble
Shifted, therefore transfer device also includes transfer assembly 31, transfer assembly 32 and transfer assembly 33, transfer assembly in the present embodiment
Structure and application principle all same, between transfer tape assemble 21 and transfer tape assemble 221 chip transmission be by transfer group
Part 31 is come what is shifted, and transfer assembly 32 is connected between transfer tape assemble 221 and transfer tape assemble 231, and transfer assembly 33 is connected
Between transfer tape assemble 231 and transfer tape assemble 241.
It is described in detail below with transfer assembly 31, transfer assembly 31 includes housing 311, motor 312, driving wheel
314th, driven pulley 319, timing belt 315, sliding block 318, main absolute position encoder 313 and from absolute position encoder 316, definitely
Position coder 313 and from absolute position encoder 316 can use individual pen absolute value encoder, individual pen absolute value encoder can
To be photoelectric encoder, magnetic encoder, resolving encoder, capacitive grating encoder.The rectangular framework of housing 311 is set and at middle part
Be provided with accommodating chamber, be provided with slide rail 317 on the first side wall of accommodating chamber, slide rail 317 is set in groove, sliding block 318 from
The slide rail 317 is passed through in accommodating chamber, sliding block 318 is arranged in slide rail 317 and can moved along slide rail 317.The He of motor 312
It is arranged on from absolute position encoder 316 on the outer wall of the second sidewall of accommodating chamber, driving wheel 314, driven pulley 319 and timing belt
315 are arranged in accommodating chamber, and driving wheel 314 is arranged on the output shaft of motor 312, main absolute position encoder 318
It is connected on the output shaft of motor, timing belt 315 is connected between driving wheel 314 and driven pulley 419, sliding block 318 is set
On timing belt 315, it is connected from absolute position encoder 316 by rotating shaft with driven pulley 319.And the diameter of driving wheel 314 with
The diameter and the number of teeth of driven pulley 319 are differed, in the present embodiment, and the diameter of driving wheel 314 is less than the diameter of driven pulley 319
Ground is set, and the number of teeth of the number of teeth less than driven pulley 319 of driving wheel 314.Motor can using servomotor, stepper motor or
DC brushless motor etc..
In order to accurately know the position of test chip 10 and sliding block 318, existing design generally by
Multi-turn absolute value encoder coordinates motor accurately to control turned position.Transfer assembly 31 stirs test chip
When, because the diameter of driving wheel is less than the diameter of driven pulley, therefore driving wheel 314 is obtained by main absolute position encoder 313
Rotational angle data, by obtaining the rotational angle data of driven pulley 319 from absolute position encoder 316, when there is step-out,
By comparing the rotation data of driving wheel and driven pulley, and according to the relation ratio of two diameters, and according to the pass between the number of teeth
System, just can calculate the number of turns that driven pulley/driving wheel is turned over, and calculate the position of current slide, and the present embodiment is used
Scheme be by two absolute value encoders, different-diameter size, the poor number of teeth, encoder resolution ratio driving wheel and driven
Wheel measures calculating, and its cost is low and just can realize the purpose and function of this case using general motor.
Then test chip 10 at transfer tape assemble 21 can be sent to transfer tape assemble exactly by sliding block 318
At 221, meanwhile, limited block is collinearly set, and has opening, sliding block 318 between two limited blocks on the width of temperature-constant plate
Just by test chip 10 between the limited block that opening enters and is pushed on transmission belt.
On the end of transfer tape assemble 221, i.e., test chip, which enters on the position of transfer tape assemble 221, is provided with first
In clean and change liquid mechanism, the present embodiment the first clean and change liquid mechanism include fluid injection component 51, fluid injection component 51 include L-type support and
Three conduits 511 on support are arranged on, conduit 511 arranges that three conduits 511 are connected with pump 14 respectively in vertical direction, leads to
Cross in the presence of pump 14, test fluid is filled into test chip 10.Test chip 10 is provided with three test positions 101, test
Position is used to place testing liquid and test fluid, and test position 101 is in groove arrangement, and sets conduction hole on adjacent two side,
Conduction hole 102 is located on length direction, and conduction hole 103 is located on width, in the present embodiment the curved groove of conduction hole
Set.During practical application, test fluid is uniformly distributed in test position 101 during filling test fluid in order to allow, can pass through
The initial filling position of the cooperation of transfer tape assemble 221 and pump 14, i.e. conduit 511 is located at the opposite side of conduction hole 102, Ran Houchuan
Sending the mobile test chip 10 of band component 221, conduit 511 continues to fill test fluid, as test chip 10 is moved, conduit 511 simultaneously
Close to conduction hole 102, the then stopping of conduit 511 filling then realizes that conduit 511 is equably filled on test position 101
Test fluid.Then enter test reaction device under the drive of transfer tape assemble 221, carry out the stop or walking of preset time,
Period, test fluid and prepare liquid were fully reacted also under default temperature and humidity state.
When test chip 10 is sent to the other end by transfer tape assemble 221, then by transfer assembly 32 by test chip
On 10 transfer conveyor belt components 231, the second clean and change liquid mechanism is provided with the end for moving transfer tape assemble 231.Specific ginseng
According to Figure 11, the second clean and change liquid mechanism 52 includes lifting assembly, fluid injection component, imbibition component and chip position, and chip position is used to put
Put test chip 10.Lifting assembly includes motor, eccentric wheel, encoder 523, support 524, sliding block 528 and slide rail 526,
Encoder is connected with motor, and motor driving eccentric wheel is circumferentially moved around the output shaft of motor, and sliding block is provided with waist
Circular hole, eccentric wheel coordinates with waist round hole, and sliding block 528 is matched somebody with somebody with slide rail 526 and merges and can be slided along slide rail.
Imbibition component includes three conduits 529, and three conduits 529 are connected with pump, and it is used for the side from test chip 10
Conduction hole draw test fluid, three conduits 529 are arranged on 527 on sliding block.Lifting assembly is connected with imbibition component and can driven
Imbibition component relative to chip position lifting moving, realize when chip in place after to test chip progress imbibition processing.
Fluid injection component includes support 521, three conduits 522 and three conduits 528, and support 521 is fixedly provided in shell
In 11, three conduits 522 are connected with pump, are set between conduit 522 and horizontal plane in filling inclination angle, inclination angle is at an acute angle sets for filling
Put, the outlet of conduit 522 is located relative on the side of conduction hole 103, i.e. the entrance position of the outlet of conduit 522 and conduit 529
In on the both sides of test position 101.
Three conduits 528 are arranged on sliding block 528, and three conduits 528 are also to be moved, conduit with the movement of sliding block 528
528 position is accordingly set with the conduction hole 102 of test chip, and three conduits 528 are used to fill towards test chip 10
Test fluid
Second clean and change liquid mechanism 52 also includes the separate sheet 525 for being arranged on the both sides of conduit 529, and separate sheet 525 is set in strip
Put, separate sheet 525 is being provided with inclined guide surface on the end of support 521, and two separate sheets 525 are positioned at test chip
Top and to the position-limiting action on test chip 10 its vertical direction, its reason be conduit 528 when drawing test fluid, suction
Test chip 10 may be sucked up, therefore effectively test chip can be carried out by separate sheet 525 spacing, it is ensured that conduit
528 efficiently separate with test chip.
When second clean and change liquid mechanism 52 is operated, the end in transfer tape assemble 231, test chip 10 is first
When entering the chip position of clean and change liquid mechanism, test chip 10 is to be mounted with test fluid, and the second clean and change liquid mechanism 52 is being entered
Row needs first to carry out cleaning step when changing second of test fluid.
Therefore the control method of the second clean and change liquid mechanism 52 includes：
Test fluid in test chip 10 is drawn by conduit 529；
Then, cleaning solution is filled by conduit 522, meanwhile, conduit 529 draws cleaning solution simultaneously, due to conduit 522 and conduit
529 on the both sides, thus incline direction fill cleaning solution, and produce convection current on the test position of test chip, then effectively
Improve cleaning force.
Then, conduit 522 stops filling cleaning solution, and conduit 529 draws cleaning solution clean；
Finally, second of test fluid is filled to test chip by conduit 527, during filling, test chip 10 is by transfer tape assemble
231 are driven, and with the movement of test chip 10, conduit 527 is filled.
As test chip is driven the test reaction device of entrance temperature-constant plate 233 by transfer tape assemble 231, during by presetting
Between, the reaction of temperature and humidity, test chip is sent to the other end, then again by transfer assembly 32, by test chip
Transfer tape assemble 241 is transferred to from transfer tape assemble 231.The starting end of transfer tape assemble 241 is provided with the second cleaning to change
Liquid mechanism 52, subsequent second clean and change liquid mechanism 52 performs above-mentioned control method to test chip, and second of test fluid is inhaled
Take, and after cleaning, add the third test fluid.
Test chip is driven by transfer tape assemble 241 again, into the test reaction device of temperature-constant plate 243, by default
Time, the reaction of temperature and humidity, test chip are sent to the other end, finally by the 3rd clean and change liquid mechanism 53.
Reference picture 12 to Figure 14, clean and change liquid mechanism 53 include lifting assembly, translation component, air blowing component, fluid injection component,
Imbibition component and chip position, translation component include motor 531, support 532, eccentric wheel 539, sliding block 534 and slide rail 533,
The output shaft of motor 531 is connected with eccentric wheel 539, and eccentric wheel 539 deviates the axis setting of output shaft, motor
531 driving eccentric wheels 539 are circumferentially moved around output shaft, and sliding block 534 is provided with waist round hole 538, eccentric wheel 539 and waist round hole
538 coordinate, and support 532 is in arcuation setting, and slide rail 534 and motor 531 be fixedly connected on support 532, and slide rail 533 is along water
Square extend to the direct of travel of arrangement and test chip, sliding block 534 and slide rail 533 are with merging and can be slided along slide rail 533, air blowing
Component is arranged on sliding block 534.
Air blowing component includes gas passage and the intake interface 536 of compressed air, and air pump interface 536 and gas passage connect
Connect, gas passage is surrounded by pipeline block, the gas passage of connection, the first end connection air inlet connecting of gas passage are opened up in pipeline block
Mouthfuls 536, the second end of gas passage is provided with three gas outlets 536, gas outlet 536 towards conduit 545 entrance tilt and set
Put, the gas outlet 536 of gas passage is used to blow to the chip surface on chip position.
Lifting assembly includes motor 523, support 524, eccentric wheel, sliding block 527 and slide rail 526, motor driving
Eccentric wheel is circumferentially moved around the output shaft of motor, and sliding block is provided with waist round hole, and eccentric wheel coordinates with waist round hole, the He of slide rail 527
Motor 523 is fixedly installed on support 524 and is arranged in a vertical direction, and sliding block 544 and slide rail 543 are with merge can be along slide rail
543 are slided, and imbibition component is arranged on sliding block 544.
Imbibition component includes three conduits 545, the entrance of conduit 545 set towards chip position and for draw test fluid and
Cleaning solution, three conduits 545 simultaneously can accordingly move down into drawing for test chip 10 with the lifting moving of sliding block 544, its entrance
In discharge orifice 102.The outlet of conduit 546 and the entrance of conduit 545 are arranged on the rear side of the bearing of trend of slide rail 533.
3rd clean and change liquid mechanism 53 is also including the separate sheet 537 for being arranged on the both sides of conduit 545, separate sheet and positioned at lifting
Between component and chip position, separate sheet 537 is set in strip, and separate sheet 537 is provided with the end of close support 532 inclines
Oblique guide surface, two separate sheets 537 are located at the top of test chip and to the spacing work on test chip 10 its vertical direction
With, its reason be conduit 545 when drawing test fluid, suction may suck up test chip 10, therefore pass through separate sheet 537
Effectively test chip can be carried out spacing.
Fluid injection component includes three conduits 546, and three conduits 546 are arranged on the position between two separate sheets 537, and three
Be set in parallel between individual conduit 546 and horizontal plane, certainly can also angle at an acute angle filling inclination angle set, three conduits
546 are used for towards chip position filling cleaning solution.
3rd clean and change liquid mechanism 53 changes liquid process step in the last cleaning of processing test chip, its control method bag
Include：
Test chip 10 is sent to the chip position of the 3rd clean and change liquid mechanism 53；
Lifting assembly drops to conduit 545 in the conduction hole with test chip 10 on cooperatively position, and conduit 545, which is drawn, to be surveyed
Try the test fluid on chip 10；
Cleaning is then carried out, conduit 546 fills cleaning solution to test chip 10, while conduit 545 is also simultaneously to clear
Clean liquid is drawn；
Then conduit 546 stops filling cleaning solution；
Finally, translation Component driver air blowing component is moved towards the ground of conduit 545, and air blowing component blows towards chip position while mobile
Gas, meanwhile, conduit 545 draws the remaining cleaning solution in test chip 10, finally all draws the cleaning solution on chip position dry
Only.
Reference picture 15 and Figure 16, system block diagram and operation principles figure below in conjunction with apparatus for testing chip carry out test stream
Journey explanation.Apparatus for testing chip is usually to be used cooperatively with external computer or embedded system or single-chip computer control system,
It is also the processing module with processing operational capability can be integrated in apparatus for testing chip, the control in apparatus for testing chip
Panel or upper computer software such as computer, flat board, mobile phone etc. can be configured to parameters, the parameter of setting include temperature,
Time, humidity, transfer rate etc., additionally it is possible to pass through control panel or the operating state of apparatus for testing chip.
Apparatus for testing chip includes multiple driver elements, multiple motors and multiple encoders, such as above-mentioned embodiment institute
The function to be realized and effect are mentioned to, multiple motors are respectively used to driving transfer tape assemble, transfer assembly, for adding
The pump of note and the pump for absorption, a road motor of driver element band four and encoder, driver element are connected using Ethernet
The driving plate connect, computer is connected by communication unit with each driver element, and connected mode can be connected by the way of series connection,
Computer efficiently can be driven by encoding to each motor, at the same using communication unit and humidity sensor,
Temperature sensor and heater be controlled or information acquisition
When apparatus for testing chip is handled test chip to be tested, its control method includes：
First, thing to be detected is filled to test chip 10, is subsequently placed on the transfer tape assemble 21 of feeding area；
Then, test chip is sent to end, and by the transfer of transfer assembly 31, test chip is transferred to transfer tape assemble
On 221 end；
Then, fluid injection component 51 fills test fluid to test chip；
Then, transfer tape assemble 221 transfers to test chip, and by the test reaction device of temperature-constant plate 223, and 25
DEG C reacted 20 minutes to 120 minutes between 85 DEG C；
Then, subsequent test chip is sent to the other end, and is transferred to the end on transfer tape assemble 231 by transfer assembly 32
In portion；
Then, the second clean and change liquid mechanism 52 performs above-mentioned process step to test chip, including draws test fluid, by clear
Washing lotion is cleaned, and adds second of test fluid；
Then, transfer tape assemble 231 transfers to test chip, and by the test reaction device of temperature-constant plate 233, and 25
DEG C reacted 20 minutes to 120 minutes between 85 DEG C；
Then, subsequent test chip is sent to the other end, and is transferred to the end on transfer tape assemble 241 by transfer assembly 33
In portion；
Then, above-mentioned process step is being performed to test chip by the second clean and change liquid mechanism 52, including is drawing second and surveyed
Test solution, is cleaned by cleaning fluid, adds the third test fluid；
Then, transfer tape assemble 241 transfers to test chip, and by the test reaction device of temperature-constant plate 243, and 25
DEG C reacted 20 minutes to 120 minutes between 85 DEG C；
Finally, above-mentioned process step is performed to test chip by the 3rd clean and change liquid mechanism 53, including draws nitrite ion the
Three test fluids, are cleaned by cleaning fluid, and blowing is blotted, and are finally exported from chip outlet 111.Pass through the chip-detecting apparatus of this case
Artificial detection is instead of, continual continuous detection can be achieved, detection efficiency is greatly improved, avoids the occurrence of detection failure.
It is further to note that this front side, rear side and left and right sides in embodiment is referred to along transfer tape assemble
Direct of travel, the direction of advance of transfer tape assemble is front side, and the opposite side of front side is rear side, therefore for the second clean and change liquid
The conduit 528 of mechanism 52 is to be located at front side, and conduit 522 is to be located at rear side and the side setting that keeps left, and conduit 529 is to be located at right side；
Conduit 545 and conduit 546 in second clean and change liquid mechanism 52 are to be located at rear side, and air blowing component is then to be located at front side.
The first clean and change liquid mechanism 51 in reference picture 17, above-described embodiment and the second clean and change liquid mechanism 52 is replaceable makes
With the 4th clean and change liquid mechanism 54, and the replaceable use test chip 20 of test chip 10 in above-described embodiment.
Specifically, the second clean and change liquid mechanism 54 includes lifting assembly, fluid injection component, imbibition component and chip position, chip
Position is used to place test chip 20.Test chip 20 is provided with three test positions 201, and test position is used to place testing liquid and survey
Test solution, test position 201 is in groove arrangement, and setting conduction hole 202,203, in the present embodiment drainage on relative two side
The curved groove in hole is set.
Lifting assembly includes motor, eccentric wheel, encoder 543, support 544, sliding block 548 and slide rail 546, encoder
It is connected with motor, motor driving eccentric wheel is circumferentially moved around the output shaft of motor, and sliding block is provided with waist round hole, partially
Heart wheel coordinates with waist round hole, and sliding block 548 is matched somebody with somebody with slide rail 546 and merges and can be slided along slide rail.
Imbibition component includes three conduits 549, and three conduits 549 are connected with pump, and it is used for the drainage from test chip 20
Draw test fluid in hole.Fluid injection component includes three conduits 541 being vertically arranged side by side and the conduit 542 set laterally side by side, three
Individual conduit 549 and three conduits 541 are connected with pump and fill test fluid, three conduits 549 and three towards test chip 20 respectively
Individual conduit 541 is arranged on sliding block 548, also, the outlet of the entrance and conduit 541 of conduit 549 is used for towards the test core
The middle part of piece, the outlet of conduit 542 is used for the sidepiece towards test chip, and conduit 542 is inclined or parallel to horizontal plane
Set.The position of conduit 549 is accordingly set with the conduction hole 202,203 of test chip, and lifting assembly is used to drive conduit
549 and the lifting moving of conduit 541, realize when chip in place after imbibition processing and filling processing are carried out to test chip.
When 4th clean and change liquid mechanism 54 is operated, the end in transfer tape assemble, test chip 20 is being entered
During the chip position of clean and change liquid mechanism, discriminably the first test fluid of progress absorption, the second test fluid of filling, absorption second is tested
The 3rd test fluid of liquid and filling, then in test reaction device is entered.
Certain 3rd clean and change liquid mechanism 53 is also to be changed, can be by the 4th clean and change liquid mechanism 54 with blowing
Changed after component combination, its function and effect are identicals, are also that by the purpose of the present invention.
By main transport mechanism, clean and change liquid mechanism and test reaction device, test chip automatic moving is realized
It is dynamic, and the function such as insulation, the moisturizing having using test reaction device so that transmission mechanism can by budget speed and it is default when
Between pass through test reaction device, and can by clean and change liquid mechanism to test chip realize filling test fluid and draw survey
Test solution.Multi-angle servicing fluids, imbibition and aeration cleaning can be realized using clean and change liquid mechanism simultaneously, then realizes that test chip is high
The accurate detection of effect.
Above-described embodiment is the preferred embodiment of this case, has a variety of changes, such as above-mentioned reality among practical application
Apply in example and accurately controlled using motor, be naturally also can using general motor cooperation position sensor come
Realize accurate transmission and position, and for transfer assembly, can also general electricity in addition to the transfer assembly in such as above-mentioned embodiment
Machine also can be achieved on the transfer between different transmission belt components to test chip with movable slider.
In addition, the walking path of the test chip of above-described embodiment is set with spiraling in wriggling, or set in square-wave-shaped
Put, it is can to efficiently reduce the volume of apparatus for testing chip, be naturally also to set along a straight line, this is just no longer needed
Want transfer assembly, it is only necessary to add clean and change liquid mechanism and test reaction device in position.
Claims (10)
1. test reaction device, it is characterised in that：Including lid, temperature-constant plate and heater, the lid is covered in the perseverance
The top of warm plate, the temperature-constant plate is provided with liquid tank, the lid and the liquid tank on the end face towards the lid
Between form reaction chamber, the heater includes multiple heating plates for abutting against the temperature-constant plate bottom surface, it is multiple described plus
Bearing of trend arrangement of the backing along the temperature-constant plate.
2. test reaction device according to claim 1, it is characterised in that：
The lid is towards being provided with multiple grooves being arranged side by side, multiple equal edges of groove on the end face of the temperature-constant plate
The bearing of trend arrangement of the temperature-constant plate.
3. test reaction device according to claim 1, it is characterised in that：
The liquid tank is connected by multiple conduits and formed.
4. test reaction device according to claim 1, it is characterised in that：
The test reaction device also includes water inlet and discharge outlet, and the water inlet and the discharge outlet are separately positioned on described
On two ends of liquid tank.
5. test reaction device according to claim 4, it is characterised in that：
The distance between bottom land of the discharge outlet and the liquid tank is preset height.
6. the test reaction device according to any one of claim 1 to 5, it is characterised in that：
Both sides on the bearing of trend of the temperature-constant plate are provided with chute, and the chute is used to install transfer tape assemble.
7. chip-detecting apparatus, it is characterised in that including
Transport mechanism, the transport mechanism is used to transport test chip；
Clean and change liquid mechanism, the clean and change liquid mechanism is used for towards the test chip filling in the transport mechanism or draws survey
Test solution；
Test reaction device, the test reaction device is using the test reaction dress described in any one of the claims 1 to 6
Put, the transport mechanism passes through the reaction chamber.
8. chip-detecting apparatus according to claim 7, it is characterised in that：
The transport mechanism includes multiple transfer tape assembles；
Multiple transfer tape assembles are provided with chip and place position, the conveyer belt in being arranged side by side on the transfer tape assemble
Component passes through the reaction chamber.
9. chip-detecting apparatus according to claim 7, it is characterised in that：
The clean and change liquid mechanism includes fluid injection component and imbibition component, and the clean and change liquid mechanism is arranged on the temperature-constant plate
On end；
The fluid injection component includes the first conduit, and first conduit is arranged on the top of the transport mechanism, the fluid injection group
Part is used to fill test fluid towards the test chip in the transport mechanism by first conduit；
The imbibition component includes the second conduit, and second conduit is arranged on the top of the transport mechanism, the imbibition group
Part is used to draw test fluid towards the test chip in the transport mechanism by second conduit.
10. the control method of chip-detecting apparatus, it is characterised in that the chip-detecting apparatus uses the claims 7 to 9
Chip-detecting apparatus described in any one；
The control method includes：
The clean and change liquid mechanism fills test fluid to the test chip in the transport mechanism；
The transport mechanism drives the test chip to pass through the test reaction device；
The clean and change liquid mechanism draws test fluid to the test chip in the transport mechanism.
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| CN201710501333.6A CN107144698B (en) | 2017-06-27 | 2017-06-27 | Test reaction device, chip-detecting apparatus and its control method |
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| CN201710501333.6A CN107144698B (en) | 2017-06-27 | 2017-06-27 | Test reaction device, chip-detecting apparatus and its control method |
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| CN107144698B CN107144698B (en) | 2018-06-26 |
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Cited By (1)
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| CN113759136A (en) * | 2021-09-09 | 2021-12-07 | 珠海圣美生物诊断技术有限公司 | Full-automatic sample slide treatment instrument and sample slide treatment method |
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| CN107144698B (en) | 2018-06-26 |
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