CN113759142A - Blood sampling method and blood sampling device - Google Patents
Blood sampling method and blood sampling device Download PDFInfo
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- CN113759142A CN113759142A CN202010501406.3A CN202010501406A CN113759142A CN 113759142 A CN113759142 A CN 113759142A CN 202010501406 A CN202010501406 A CN 202010501406A CN 113759142 A CN113759142 A CN 113759142A
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- hollow structure
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- 238000000034 method Methods 0.000 title claims abstract description 33
- 238000010241 blood sampling Methods 0.000 title claims description 31
- 238000012360 testing method Methods 0.000 claims abstract description 194
- 210000002966 serum Anatomy 0.000 claims abstract description 61
- 238000000926 separation method Methods 0.000 claims abstract description 48
- 210000004369 blood Anatomy 0.000 claims abstract description 40
- 239000008280 blood Substances 0.000 claims abstract description 40
- 230000000149 penetrating effect Effects 0.000 claims abstract 2
- 238000004140 cleaning Methods 0.000 claims description 11
- 238000002955 isolation Methods 0.000 claims description 11
- 238000012546 transfer Methods 0.000 claims description 7
- 238000007664 blowing Methods 0.000 claims description 6
- 238000011166 aliquoting Methods 0.000 claims description 5
- 230000000087 stabilizing effect Effects 0.000 claims description 3
- 239000012528 membrane Substances 0.000 abstract description 3
- 238000005070 sampling Methods 0.000 description 12
- 238000001514 detection method Methods 0.000 description 7
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000011109 contamination Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 230000000712 assembly Effects 0.000 description 3
- 238000000429 assembly Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
Abstract
A blood sample separating method and a blood sample separating device are provided, the blood sample separating device comprises a sample separating needle, a puncture needle and a driving mechanism for connecting the sample separating needle and the puncture needle, wherein the puncture needle is provided with a hollow structure, and the sample separating needle is arranged in the hollow structure in a penetrating way; the method comprises the following steps: the driving mechanism drives the puncture needle to penetrate through a test tube cover of the target test tube and drives the sample separation needle to move downwards in the hollow structure of the puncture needle; the sample separating needle sucks serum in a target test tube; the drive mechanism drives the sample separation needle to move upwards in the hollow structure of the puncture needle, and drives the puncture needle to move upwards to leave the test tube cover of the target test tube. Pass the test tube lid of test tube through the puncture needle thorn, then divide the appearance needle to be in downstream is in order to absorb the serum in the test tube among the hollow structure of pjncture needle to need not to get the test tube lid and just can absorb the blood sample, the test tube lid still covers on the body of test tube after absorbing the blood sample moreover, need not add the lid or add the membrane, can effectively avoid biological safety risk, reduces work load, saves the cost.
Description
Technical Field
The application relates to the technical field of medical instruments, in particular to a blood sample separating method and blood sample separating equipment.
Background
For people of different types and different conditions, when a hospital collects blood, different strategies are often adopted, such as infants, weak old people or critical patients, the hospital often draws less blood for the people, and avoids great negative influence on the bodies of the people caused by excessive blood drawing, detection items of the patients are not few, the strategy adopted by the hospital is to sample the blood collection samples of the people so as to support parallel tests of different business projects, and not each business project consumes one or more tubes of the blood collection samples, so that sample sampling is common in the hospital.
The sample is divided into manual sample division and automatic instrument sample division, the manual sample division efficiency is low, the sample division result is inaccurate, the operation teacher also has infected risks for highly pathogenic samples, the automatic instrument sample division efficiency is high, the sample division result is accurate, and the infected risks in the sample division link are greatly reduced because the operation teacher does not directly operate. However, for biochemical immunization services, the current sample separation is premised on the sample needing to be uncapped so that the separation instrument can draw serum from the test tube. Therefore, at present, the sample needs to be subjected to sample separation in advance, the possibility that the sample volatilizes to the air exists in the sample removing process and after the sample is subjected to the sample removing, the sample is subsequently subjected to capping/film adding so as to prevent volatilization, dilution and storage or prevent biological pollution caused by direct discarding, the work flow is increased, the workload is increased, the cost is increased, and the biological safety risk is increased.
Disclosure of Invention
Based on this, the application provides a blood sample separating method and blood sample separating equipment, aims at solving the technical problem that the existing sample separating needs to be uncovered and the like.
In a first aspect, the present application provides a blood sample separation method, which is used for a blood sample separation device, where the blood sample separation device includes a sample separation needle, a puncture needle, and a driving mechanism connecting the sample separation needle and the puncture needle, where the puncture needle is provided with a hollow structure, and the sample separation needle is inserted into the hollow structure;
the method comprises the following steps:
the driving mechanism drives the puncture needle to penetrate through a test tube cover of a target test tube and drives the sample separation needle to move downwards in the hollow structure of the puncture needle;
the sample dividing needle sucks the serum in the target test tube;
the driving mechanism drives the sample dividing needle to move upwards in the hollow structure of the puncture needle, and drives the puncture needle to move upwards to leave the test tube cover of the target test tube.
In a second aspect, embodiments of the present application provide a blood sampling apparatus, including:
the puncture needle is provided with a hollow structure;
the sample separating needle penetrates through the hollow structure and absorbs serum in a target test tube;
the driving mechanism is connected with the sample distribution needle and the puncture needle, drives the puncture needle to penetrate through the test tube cover of the target test tube, drives the sample distribution needle to move downwards in the hollow structure of the puncture needle, drives the sample distribution needle to move upwards in the hollow structure of the puncture needle, and drives the puncture needle to move upwards to leave the test tube cover of the target test tube.
The embodiment of the application provides a blood divides kind method and blood to divide kind equipment, passes the test tube lid of target test tube through the puncture needle thorn, then divides the appearance needle to be in downstream is in order to absorb the serum in the target test tube in the hollow structure of pjncture needle to need not to take off the test tube lid and just can absorb the blood sample, and the test tube lid still covers on the body of target test tube after absorbing the blood sample, need not add the lid or add the membrane, can effectively avoid biological safety risk, reduce work load, save the cost.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure of the embodiments of the application.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic flow chart of a blood sampling method according to an embodiment of the present disclosure;
fig. 2 is a schematic structural diagram of a blood sampling apparatus according to an embodiment of the present application;
FIG. 3 is a schematic view of the piercing needle and the aliquoting needle above the target test tube;
FIG. 4 is a schematic view of the needle beginning to pass through the cap of the tube;
FIG. 5 is a schematic view of the piercing needle passing through the cover of the tube;
FIG. 6 is a schematic view of the needle moving down in the needle;
FIG. 7 is a schematic view of the lower end of the sampling needle extending into the serum;
FIG. 8 is a schematic view of the dispensing needle and the piercing needle being removed from the vial cap;
fig. 9 is a schematic structural diagram of a blood sampling apparatus according to another embodiment of the present application.
Reference numerals: 100. a blood sampling device; 110. a sample separating needle; 120. puncturing needle; 121. a hollow structure; 122. an isolation structure; 123. an electromagnetic valve; 130. a drive mechanism; 140. a blowing mechanism;
10. a target test tube; 11. a test tube cover; 21. a serum layer; 201. the surface of the serum; 22. a blood cell layer.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some, but not all, embodiments of the present application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The flow diagrams depicted in the figures are merely illustrative and do not necessarily include all of the elements and operations/steps, nor do they necessarily have to be performed in the order depicted. For example, some operations/steps may be decomposed, combined or partially combined, so that the actual execution sequence may be changed according to the actual situation.
Some embodiments of the present application will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.
Referring to fig. 1, fig. 1 is a schematic flow chart of a blood sampling method according to an embodiment of the present disclosure. The blood sampling method can be applied to blood sampling equipment and is used for processes of sucking serum from a test tube and the like.
Referring to fig. 2, fig. 2 is a schematic structural diagram of a blood sampling apparatus 100 according to an embodiment of the present disclosure.
As shown in fig. 2, the blood sampling apparatus 100 includes a sampling needle 110, a puncture needle 120, and a drive mechanism 130 connecting the sampling needle 110 and the puncture needle 120.
In some embodiments, the sample-dispensing needle 110 includes a needle body and a needle head arranged along a length axis, wherein a fluid passage is arranged in the needle body and the needle head, the fluid passage extends along the length axis, and one end of the fluid passage is open and arranged on the needle head.
The sample dispensing needle 110 is used to aspirate serum in the target test tube 10. When a negative pressure is created in the fluid path, the dispensing needle 110 is able to draw a blood sample, such as serum, from the opening.
Specifically, as shown in fig. 2 to 7, the puncture needle 120 is provided with a hollow structure 121, and the sample dispensing needle 110 is inserted into the hollow structure 121. For example, the sample dispensing needle 110 can move relative to the puncture needle 120 within the hollow structure 121.
In some embodiments, the drive mechanism 130 includes a first drive assembly for driving the movement of the dispensing needle 110, and a second drive assembly for driving the movement of the puncture needle 120; it is understood that the driving mechanism 130 can drive the dispensing needle 110 and the puncture needle 120 to move separately, and for example, the driving mechanism 130 can drive the dispensing needle 110 and the puncture needle 120 to move simultaneously.
For example, the first and second driving assemblies may drive the sample dispensing needle 110 and the puncture needle 120 to move through an electromagnetic driving manner, or the first and second driving assemblies may drive the sample dispensing needle 110 and the puncture needle 120 to move through a pneumatic manner, or the first and second driving assemblies may drive the sample dispensing needle 110 and the puncture needle 120 to move through a motor rotation manner.
As shown in fig. 1, the blood sampling method according to the embodiment of the present application includes steps S110 to S130.
Step S110, the driving mechanism 130 drives the puncture needle 120 through the test tube cap 11 of the target test tube 10, and drives the dispensing needle 110 to move downward in the hollow structure 121 of the puncture needle 120.
In some embodiments, the method further comprises: moving the target test tube 10 to a preset test tube position, and righting and stabilizing; the driving mechanism 130 drives the puncture needle 120 and the sampling needle 110 to move in the horizontal direction to above the target test tube 10.
In some embodiments, the blood sampling apparatus 100 further comprises a test tube transfer device for moving the test tube to a predetermined test tube position.
Illustratively, the test tube transfer device carries the target test tube 10, moves the target test tube 10 to a preset test tube position, and stabilizes.
Illustratively, the blood sampling device 100 receives a test tube that the user has placed in, and grabs the test tube into the test tube transfer device, or the blood sampling device 100 directly receives a test tube from another device into the test tube transfer device to move the test tube to a predetermined test tube position by the test tube transfer device.
For example, the blood sampling apparatus 100 further includes a scanning device (not shown in the figure) disposed at the barcode scanning position, the test tube transferring device can transfer the test tube to the barcode scanning position, and the scanning device identifies a sample identifier, such as a serial number ID, of the test tube, so that the blood sampling apparatus 100 can obtain information of the current test tube, for example, it can also determine that a blood sample in the test tube needs to be sampled into at least a plurality of sub-test tubes according to the information of the current test tube, and can also determine a sample volume of each sub-test tube.
Exemplarily, blood divides appearance equipment 100 still includes the test tube and rights steady mechanism, and this test tube is right steady mechanism and is set up at predetermined test tube position, and after this test tube position was arrived to the test tube, the test tube was right steady mechanism and can be right steady with the test tube to divide the appearance to the blood sample in this test tube.
Illustratively, the test tube currently requiring a sample splitting operation is the target test tube 10.
In some embodiments, the driving mechanism 130 also drives the puncture needle 120 and the aliquoting needle 110 to move in the horizontal direction to above the target test tube 10.
Illustratively, the blood sampling apparatus 100 may have test tubes at one or more test tube positions, and the blood sampling apparatus 100 may determine information about the test tubes at the test tube positions and determine the current target test tube 10 based on the information.
Illustratively, the drive mechanism 130 can drive the puncture needle 120 or the aliquoting needle 110 to move up or down at the test tube position.
As shown in fig. 3, the puncture needle 120 and the sorting needle 110 are located above the target test tube 10.
As shown in fig. 3 to 5, the driving mechanism 130 drives the puncture needle 120 through the test tube cap 11 of the target test tube 10.
The test tube cover 11 may also be called a test tube cap, and may be made of rubber, for example, to prevent the blood sample from being contaminated or being released outside to cause contamination.
In some embodiments, the drive mechanism 130 drives the puncture needle 120 downward, approaching the tube cap 11, abutting the tube cap 11 and passing through the tube cap 11.
Illustratively, the test tube cover 11 is provided with an insertion portion, and the puncture needle 120 is inserted through the insertion portion of the test tube cover 11. Illustratively, the thickness of the insertion portion is smaller than other areas on the test tube cover 11 to facilitate insertion. The insertion portion is, for example, a circular region.
Illustratively, when the driving mechanism 130 drives the puncture needle 120 to penetrate through the test tube cover 11, the dispensing needle 110 may be driven to move downwards at the same time, for example, the puncture needle 120 and the dispensing needle 110 are kept relatively still; of course, the drive mechanism 130 may not drive the dispensing needle 110 downward when the puncture needle 120 is driven through the vial cover 11.
In some embodiments, the driving mechanism 130 drives the dispensing needle 110 to move downward in the hollow structure 121 of the puncture needle 120 while the driving mechanism 130 drives the puncture needle 120 to pass through the test tube cap 11 of the target test tube 10, or after the driving mechanism 130 drives the puncture needle 120 to pass through the test tube cap 11 of the target test tube 10.
Illustratively, as shown in fig. 5 to 7, the driving mechanism 130 drives the sample dispensing needle 110 to move downward in the hollow structure 121 of the puncture needle 120 at different stages.
In some embodiments, as shown in fig. 3-7, the target tube 10 is a serum-filled target tube 10. Illustratively, the blood contained in the target tube 10 is layered into a blood cell layer 22 and a serum layer 21, and some or all of the serum is required to be extracted from the target tube 10.
Illustratively, the driving mechanism 130 drives the puncture needle 120 through the tube cap 11 of the target tube 10, including: the driving mechanism 130 drives the puncture needle 120 through the tube cap 11 of the target tube 10, and the lower end of the puncture needle 120 is higher than the surface 201 of the serum in the target tube 10.
As shown in fig. 5 to 7, the lower end of the puncture needle 120 is higher than the surface 201 of the serum in the target test tube 10, for example, the puncture needle 120 moves only a distance below the test tube cap 11 without contacting the serum sample liquid, so that the air pressure inside the target test tube 10 is communicated with the atmosphere.
Illustratively, as shown in fig. 5 to 7, the driving of the sample dispensing needle 110 downward in the hollow structure 121 of the puncture needle 120 includes: the sample-dispensing needle 110 is driven to move downward in the hollow structure 121 of the puncture needle 120 until the lower end of the sample-dispensing needle 110 protrudes into the serum in the target test tube 10.
As shown in fig. 7, the lower end of the sample-dispensing needle 110 extends into the serum in the target test tube 10.
For example, the blood sampling apparatus 100 may receive the capacitance signal output by the sampling needle 110, output a corresponding voltage signal after conversion and amplify the voltage signal according to a set gain to output a gain voltage signal, and compare the gain voltage signal with a preset voltage threshold and determine whether the sampling needle 110 contacts the surface 201 of the serum in the target test tube 10. After the sample separation needle 110 contacts the surface 201 of the serum in the target test tube 10, the sample separation needle 110 is driven to continue to move downwards by a preset distance, so that the lower end of the sample separation needle 110 extends into the serum in the target test tube 10. For the liquid level detection method, it can be understood by those skilled in the art that the detection of the surface 201 of the serum can be performed by other methods besides the detection of the surface 201 of the serum by using the capacitance change, for example, the detection of the surface 201 of the serum by using the resistance or inductance change. Accordingly, a sample dispensing needle 110 adapted to the surface 201 detection method of serum should be used. According to the electrical property change of the sample distributing needle 110, the liquid level detection signal which is changed along with the electrical property change of the sample distributing needle 110 can be determined to the processor.
Illustratively, the driving of the sample separation needle 110 to move downwards in the hollow structure 121 of the puncture needle 120 until the lower end of the sample separation needle 110 extends into the serum in the target test tube 10 includes: detecting whether the lower end of the sample separating needle 110 touches the surface 201 of the serum in the target test tube 10 while driving the sample separating needle 110 to move downwards; after the lower end of the sample separation needle 110 touches the surface, the sample separation needle 110 is driven to continue to move downwards for a first preset distance, and the first preset distance is determined according to the preset serum suction amount.
For example, the blood sampling apparatus 100 may determine the amount of blood drawn from the target test tube 10 this time, i.e., a serum draw amount, based on the information of the target test tube 10, determine a first preset distance for the lower end of the sampling needle 110 to move downward after touching the surface, and control the driving mechanism 130 to drive the sampling needle 110 to move downward by the first preset distance.
Step S120, the sample separating needle 110 sucks the serum in the target test tube 10.
In some embodiments, after the lower end of the sample separation needle 110 extends into the serum in the target test tube 10, the sample separation needle 110 sucks the serum in the target test tube 10.
Illustratively, when the sample distribution needle 110 moves downwards and continues to move downwards for a first preset distance after the lower end touches the surface, the sample distribution needle 110 can suck a corresponding serum suction amount, and a blood sample can be sucked quantitatively.
In some embodiments, after the sample separation needle 110 is driven to move downwards in the hollow structure 121 of the puncture needle 120 until the lower end of the sample separation needle 110 touches the surface 201 of the serum in the target test tube 10, the sample separation needle 110 sucks the serum in the target test tube 10, and the blood sample can be sucked more quickly. For example, the blood sampling device 100 can detect the amount of serum drawn by the sampling needle 110, so as to quantitatively draw the blood sample.
In step S130, the driving mechanism 130 drives the dispensing needle 110 to move upward in the hollow structure 121 of the puncture needle 120, and drives the puncture needle 120 to move upward to be away from the test tube cap 11 of the target test tube 10.
In some embodiments, as shown in fig. 8, after the sample-separating needle 110 sucks a certain amount of serum, the driving mechanism 130 drives the sample-separating needle 110 to move upward in the hollow structure 121 of the puncture needle 120, and then drives the puncture needle 120 to move upward to be away from the test tube cap 11 of the target test tube 10.
Illustratively, as shown in fig. 8, after the driving mechanism 130 drives the sample dispensing needle 110 to move upward for a certain distance in the hollow structure 121 of the puncture needle 120, the sample dispensing needle 110 and the puncture needle 120 can be driven to move upward simultaneously.
Illustratively, after the driving mechanism 130 drives the sample dispensing needle 110 to move upward to a preset height, the puncture needle 120 is driven to move upward to be away from the test tube cap 11 of the target test tube 10. After the puncture needle 120 leaves the tube cap 11 of the target tube 10, the tube cap 11 is also capped on the body of the target tube 10.
In some embodiments, the separation needle 110 sucks in the isolation air while the separation needle 110 is driven to move upward in the hollow structure 121 of the puncture needle 120 and when the distance between the lower end of the separation needle 110 and the surface 201 of the serum in the target test tube 10 reaches a second preset distance.
Illustratively, after the sample is sucked, the sample separating needle 110 is lifted, and the air is sucked after the sample separating needle is lifted a certain distance away from the liquid level in the lifting process, so that the sample liquid can be prevented from being thrown out when the sample separating needle 110 moves upwards and/or horizontally, the sample can be ensured to remain on the outer wall of the needle point as little as possible, and the sample can be prevented from being stained on the inner wall of the puncture needle 120.
In some embodiments, the dispensing needle 110 is quantitatively vented when the dispensing needle 110 is driven to move upward in the hollow structure 121 of the puncture needle 120 and when the distance between the lower end of the dispensing needle 110 and the surface 201 of the serum in the target test tube 10 reaches a second preset distance.
Illustratively, in the lifting process of the sample distributing needle 110, the inner wall of the sample distributing needle 110 is quantitatively exhausted, so that the residue of the outer wall of the sample distributing needle 110 can be reduced, and meanwhile, the effective air section of the inner wall of the sample distributing needle 110 is guaranteed to be isolated.
In some embodiments, the outer wall of the dispensing needle 110 and the inner wall of the puncture needle 120 have a space therebetween, which can ensure that the air pressure inside the test tube is communicated with the atmosphere.
In other embodiments, as shown in fig. 9, a spacer 122 is provided between the outer wall of the needle 110 and the inner wall of the needle 120.
Illustratively, the isolation structure 122 may include a piston, a rubber pad, or the like. The isolation structure 122 enables the hollow structure 121 of the puncture needle 120 to form a closed space with respect to the atmosphere, and enables the needle 110 to move upward or downward in the hollow structure 121 of the puncture needle 120 while ensuring the sealing of the hollow structure 121 of the puncture needle 120. Contamination released through the hollow structure 121 of the piercing needle 120 can be reduced.
Illustratively, as shown in fig. 9, a solenoid valve 123 is provided on the sidewall of the isolation mechanism or the puncture needle 120, and the solenoid valve 123 can connect or isolate the hollow structure 121 of the puncture needle 120 from the atmosphere.
Illustratively, the method further comprises: the electromagnetic valve 123 is opened when the driving mechanism 130 drives the puncture needle 120 to penetrate through the test tube cover 11 of the target test tube 10, so that the hollow structure 121 of the puncture needle 120 is communicated with the atmosphere, the air pressure inside the target test tube 10 is communicated with the atmosphere through the hollow structure 121, the suck-back phenomenon when the sample separation needle 110 sucks the sample is prevented, the cleaning water inside the sample separation needle 110 is sucked into the test tube, the sample is diluted, and the sample sucking is inaccurate.
Illustratively, the method further comprises: the solenoid valve 123 is closed after the sample dispensing needle 110 sucks the serum in the target test tube 10. For example, controlling the solenoid valve 123 to close after the sample dispensing needle 110 finishes the sample suction to isolate the hollow structure 121 of the puncture needle 120 from the atmosphere, i.e., to close the communication between the inside of the test tube and the atmosphere, can reduce the contamination released through the hollow structure 121 of the puncture needle 120.
In some embodiments, as shown in fig. 9, the blood sampling device 100 further comprises: the blowing mechanism 140, the blowing mechanism 140 is connected with the puncture needle 120. Illustratively, the insufflation mechanism 140 includes a syringe or air pump capable of providing positive pressure to the hollow structure 121 of the introducer needle 120.
Illustratively, when the driving mechanism 130 drives the sample dispensing needle 110 to move upwards in the hollow structure 121 of the puncture needle 120, the blowing mechanism 140 blows air to the outer wall of the sample dispensing needle 110 to blow the serum on the outer wall of the sample dispensing needle 110 back to the target test tube 10, so as to reduce the residual of the serum on the outer wall of the sample dispensing needle 110 and avoid the residual of the sample on the inner wall of the puncture needle 120. Illustratively, when the sample distribution needle 110 moves upwards, the sample distribution needle 110 performs quantitative air exhaust, which can reduce the residue on the outer wall of the sample distribution needle 110 and ensure the effective air section on the inner wall of the sample distribution needle 110 to realize isolation. In addition, the enclosed space formed by the inner wall of the puncture needle 120 can be better cleaned, and cross contamination between samples can be better avoided.
In some embodiments, the blood sampling apparatus 100 further comprises a washing device (not shown) disposed at a predetermined washing position adjacent to the test tube position.
Illustratively, the method further comprises: the driving mechanism 130 drives the puncture needle 120 and the sample distributing needle 110 to move to the cleaning position in the horizontal direction, and the cleaning device cleans the puncture needle 120 and the sample distributing needle 110.
Illustratively, the drive mechanism 130 drives the puncture needle 120 and the dispensing needle 110 to move in the horizontal direction to the cleaning position. The washing device washes the puncture needle 120 and the sampling needle 110 when the driving mechanism 130 drives the puncture needle 120 and the sampling needle 110 to move to the washing position in the horizontal direction.
Illustratively, the puncture needle 120 and the sample separation needle 110 are cleaned, including: the inner and outer walls of the puncture needle 120 are cleaned, and the outer and inner walls of the sorting needle 110 are cleaned.
Illustratively, the inner and outer walls of the puncture needle 120 may be cleaned by a circular irrigation, the outer wall of the sample-dispensing needle 110 may be cleaned by a circular irrigation, and the inner wall of the sample-dispensing needle 110 may be cleaned by a syringe suction. In some embodiments, the outer wall of the needle 110 or the puncture needle 120 may also be cleaned by axially moving the swab relative to the needle 110 or the puncture needle 120.
In some embodiments, the blood sampling apparatus 100 can also prepare satisfactory test tubes, for example, a user can randomly place test tubes with the test tube caps 11 into the blood sampling apparatus 100, and the blood sampling apparatus 100 can automatically arrange the test tubes. Exemplarily, blood divides appearance equipment 100 can also print the sub test tube bar code of the preset number according to the test tube information of the main test tube of discernment automatically, paste on corresponding sub test tube, later through upright tilting mechanism with sub test tube right, on the sub test tube of retransmission divides the injection position. Of course, the cuvette may be manually placed at a corresponding location, such as a sub-cuvette dispensing location.
Illustratively, after the sample dispensing needle 110 sucks the serum in the target test tube 10, the driving mechanism 130 drives the sample dispensing needle 110 to move upward in the hollow structure 121 of the puncture needle 120 and drives the puncture needle 120 to move upward to leave the test tube cap 11 of the target test tube 10, and then the driving mechanism 130 drives the sample dispensing needle 110 and the puncture needle 120 to move in the horizontal direction to the sub-test tube dispensing position, so that the sample dispensing needle 110 and the puncture needle 120 are located above the sub-test tube.
Illustratively, the driving mechanism 130 may drive the dispensing needle 110 to move downward into the interior of the sub-tube, and the dispensing needle 110 may expel the aspirated serum into the sub-tube.
For example, the sub-test tube may not be provided with the test tube cover 11, and may be provided with the test tube cover 11. When the sub-tube is provided with the tube cap 11, the driving mechanism 130 drives the puncture needle 120 to pass through the tube cap 11 of the sub-tube, and drives the sample-dispensing needle 110 to move downward in the hollow structure 121 of the puncture needle 120, after which the sample-dispensing needle 110 can discharge the aspirated serum into the sub-tube, and after the sample discharge is completed, the driving mechanism 130 drives the sample-dispensing needle 110 to move upward in the hollow structure 121 of the puncture needle 120, and drives the puncture needle 120 to move upward to leave the tube cap 11 of the sub-tube.
For example, the aspirated serum sample may be dispensed into each sub-tube in a preset amount, and the number of the sub-tubes may be one or more, and may be preset specifically. According to the sample volume, the sample needle 110 can draw the sample of the main test tube once, or draw the sample in the main test tube for many times, so as to ensure that each sub-test tube is sampled to obtain enough serum samples.
The blood divides kind method and blood to divide kind equipment that the aforesaid embodiment of this application provided can pass the test tube lid of target test tube through the puncture needle thorn, then divide the sample needle downstream in the hollow structure of pjncture needle in order to absorb the serum in the target test tube to need not to get off the test tube lid and just can absorb the blood sample, and the test tube lid still covers on the body of target test tube after absorbing the blood sample, need not add the lid or add the membrane, can effectively avoid biological safety risk, reduce work load, save the cost. The problems that the sample separating solution provided by various manufacturers at present has biological safety risks, possibly causes infection of operating teachers, and the sample separation needs sample uncapping and then capping/film adding, so that the workload is increased and the cost is increased are solved.
In addition, the puncture needle can enable the air pressure inside the test tube to be the same as the external atmospheric pressure, so that the air pressure is kept consistent, the phenomenon that the sample is sucked backwards by the sample separating needle is avoided, the cleaning water inside the sample separating needle is sucked backwards into the test tube, the sample is diluted, and the sample suction is inaccurate; in addition, the pjncture needle cover still plays the protection in the outside of dividing the appearance needle and divides the appearance needle, avoids dividing the appearance needle to pierce alone and inserts askew problem, and the pjncture needle cover can avoid dividing remaining sample of appearance needle outer wall to be detained in the test tube outside dividing the appearance needle and cover, prevents that the test tube from covering the outer wall and remaining the risk that has the sample to lead to biological contamination.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.
It should also be understood that the term "and/or" as used in this application and the appended claims refers to and includes any and all possible combinations of one or more of the associated listed items.
While the invention has been described with reference to specific embodiments, the scope of the invention is not limited thereto, and those skilled in the art can easily conceive various equivalent modifications or substitutions within the technical scope of the invention, and these modifications or substitutions are intended to be included in the scope of the invention. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (15)
1. A blood sample separation method is used for blood sample separation equipment and is characterized in that the blood sample separation equipment comprises a sample separation needle, a puncture needle and a driving mechanism for connecting the sample separation needle and the puncture needle, wherein the puncture needle is provided with a hollow structure, and the sample separation needle is arranged in the hollow structure in a penetrating manner;
the method comprises the following steps:
the driving mechanism drives the puncture needle to penetrate through a test tube cover of a target test tube and drives the sample separation needle to move downwards in the hollow structure of the puncture needle;
the sample dividing needle sucks the serum in the target test tube;
the driving mechanism drives the sample dividing needle to move upwards in the hollow structure of the puncture needle, and drives the puncture needle to move upwards to leave the test tube cover of the target test tube.
2. The method of claim 1, wherein the driving mechanism drives the puncture needle through a tube cap of a target tube, comprising:
the driving mechanism drives the puncture needle to penetrate through a test tube cover of the target test tube, and the lower end of the puncture needle is higher than the surface of serum in the target test tube;
the driving sample separating needle moves downwards in the hollow structure of the puncture needle and comprises:
and driving the sample separating needle to move downwards in the hollow structure of the puncture needle until the lower end of the sample separating needle extends into the serum in the target test tube.
3. The method of claim 2, wherein the driving the sample separation needle downward in the hollow structure of the puncture needle until the lower end of the sample separation needle protrudes into the serum in the target test tube comprises:
detecting whether the lower end of the sample dividing needle touches the surface of the serum in the target test tube when the sample dividing needle is driven to move downwards;
and after the lower end of the sample separation needle touches the surface, driving the sample separation needle to continuously move downwards for a first preset distance, wherein the first preset distance is determined according to the preset serum suction amount.
4. The method of claim 1, wherein upon driving the dispensing needle upward in the hollow structure of the piercing needle, the method further comprises:
and when the distance between the lower end of the sample separation needle and the surface of the serum in the target test tube reaches a second preset distance, the sample separation needle sucks isolation air or carries out quantitative air exhaust.
5. The method of claim 1, wherein the outer wall of the aliquoting needle and the inner wall of the piercing needle are spaced apart.
6. The method of claim 5, wherein an isolation structure is arranged between the outer wall of the sample separation needle and the inner wall of the puncture needle, the isolation structure or the side wall of the puncture needle is provided with a solenoid valve, and the solenoid valve can enable the hollow structure of the puncture needle to be communicated or isolated from the atmosphere, and the method further comprises the following steps:
the electromagnetic valve is opened when the driving mechanism drives the puncture needle to penetrate through a test tube cover of a target test tube;
and closing the electromagnetic valve after the sample dividing needle sucks the serum in the target test tube.
7. The method according to any one of claims 1-6, further comprising:
when the sample separation needle is driven to move upwards in the hollow structure of the puncture needle, air is blown to the outer wall of the sample separation needle, so that the blood serum on the outer wall of the sample separation needle is blown back to the target test tube.
8. The method according to any one of claims 1-6, further comprising:
moving the target test tube to a preset test tube position, and righting and stabilizing;
the driving mechanism drives the puncture needle and the sample dividing needle to move to the upper part of the target test tube in the horizontal direction;
wherein the driving mechanism drives the puncture needle or the sample dividing needle to move upwards or downwards at the test tube position.
9. The method of claim 8, further comprising:
the driving mechanism drives the puncture needle and the sample dividing needle to move to a cleaning position in the horizontal direction;
and cleaning the puncture needle and the sample dividing needle.
10. A blood sampling apparatus, comprising:
the puncture needle is provided with a hollow structure;
the sample separating needle penetrates through the hollow structure and absorbs serum in a target test tube;
the driving mechanism is connected with the sample distribution needle and the puncture needle, drives the puncture needle to penetrate through the test tube cover of the target test tube, drives the sample distribution needle to move downwards in the hollow structure of the puncture needle, drives the sample distribution needle to move upwards in the hollow structure of the puncture needle, and drives the puncture needle to move upwards to leave the test tube cover of the target test tube.
11. The apparatus of claim 10, wherein the outer wall of the aliquoting needle and the inner wall of the piercing needle are spaced apart.
12. The device according to claim 11, characterized in that an isolation structure is arranged between the outer wall of the sample dividing needle and the inner wall of the puncture needle, and an electromagnetic valve is arranged on the isolation structure or the side wall of the puncture needle and can enable the hollow structure of the puncture needle to be communicated or isolated from the atmosphere;
when the driving mechanism drives the puncture needle to penetrate through a test tube cover of a target test tube, the electromagnetic valve is opened; and
and the electromagnetic valve is closed after the sample dividing needle sucks the serum in the target test tube.
13. The apparatus according to any one of claims 10-12, further comprising:
the air blowing mechanism is connected with the puncture needle;
and the blowing mechanism blows air to the outer wall of the sample dividing needle when the driving mechanism drives the sample dividing needle to move upwards in the hollow structure of the puncture needle so as to blow the blood on the outer wall of the sample dividing needle back to the target test tube.
14. The apparatus according to any one of claims 10-12, further comprising:
the test tube transfer device is used for carrying the target test tube, moving the target test tube to a preset test tube position and righting and stabilizing the target test tube;
the driving mechanism also drives the puncture needle and the sample dividing needle to move to the upper part of the target test tube in the horizontal direction;
wherein the driving mechanism drives the puncture needle or the sample dividing needle to move upwards or downwards at the test tube position.
15. The apparatus of claim 14, further comprising:
a cleaning device disposed at a cleaning position adjacent to the test tube position;
the cleaning device cleans the puncture needle and the sample separating needle when the driving mechanism drives the puncture needle and the sample separating needle to move to the cleaning position in the horizontal direction.
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| CN202010501406.3A CN113759142B (en) | 2020-06-04 | 2020-06-04 | Blood sampling method and blood sampling device |
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| CN202010501406.3A CN113759142B (en) | 2020-06-04 | 2020-06-04 | Blood sampling method and blood sampling device |
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| CN113759142B (en) | 2024-01-16 |
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