CN103575634A - Flow type instrument, liquid path system thereof and method - Google Patents
Flow type instrument, liquid path system thereof and method Download PDFInfo
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- CN103575634A CN103575634A CN201210284882.XA CN201210284882A CN103575634A CN 103575634 A CN103575634 A CN 103575634A CN 201210284882 A CN201210284882 A CN 201210284882A CN 103575634 A CN103575634 A CN 103575634A
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Abstract
The invention discloses a flow type instrument, a liquid path system thereof and a method. The liquid path system comprises a flow chamber, a sample providing device, a sheath fluid supplying device, a waste liquid absorption device and N quantifying devices, wherein pipelines of all quantifying devices are respectively communicated with the flow chamber and the sample providing device, and N is greater than or equal to 2. Each quantifying device absorbs quantified sample liquid from the sample providing device according to a set time sequence, and samples are conveyed to the flow chamber according to a predetermined pushed sample amount by at least one quantifying device at each period of time, so that the samples can be continuously fed into the flow chamber; the pushed sample amount is predetermined, so that the volume of a tested sample can be accurately quantified, the accurate counting is realized, the interruption of a testing process is avoided, the continuous measurement for the samples is realized, and the sample detection time is shortened.
Description
Technical field
The present invention relates to particle analysis field, relate in particular to a kind of streaming instrument and liquid-way system thereof.
Background technology
Streaming instrument is generally used for analysis and the statistics of fine particle, at medical field, streaming instrument is also referred to as stream type cell analyzer, for cell is classified and is counted, therefore conventionally require to draw the collection sum that is collected cell, but user need to draw the concentration information that is collected cell in sample in some cases, therefore require streaming instrument in use to part sample, need to carry out absolute counting, to draw the concentration information that is collected cell, and what all the other most of samples were needed is non-absolute counting (being common survey), to meet being collected the demand of the collection sum of cell.
For making streaming instrument can meet the requirement that is collected cell collection sum, can realize again the absolute counting function to sample, streaming instrument has two schemes at present: a kind of is absolute volume metered scheme; Another kind of for adding the standard particle measurement scheme of concentration known.
For absolute volume metered scheme, it is mainly by the quantitative method of the device parts of streaming instrument, such as fixed displacement pump etc., clearly know the volume of tested sample, then the volume divided by tested sample by the number that is collected cell recording, can obtain being collected the concentration information of cell, reach the object of absolute counting.
For another kind of scheme, in streaming instrument, there is no quantitative device parts, when user need to carry out absolute counting to sample, to the standard particle that adds concentration known in sample, this scheme need to be mixed by the standard particle of concentration known and the sample of known volume, and the standard particle concentration in sample mixed liquor can calculate.Sample mixed liquor is measured by streaming instrument, can measure standard particle number and the number that is collected cell, according to standard particle number, can go out measured body accumulated amount by inverse, then the measured body accumulated amount divided by sample mixed liquor by the number that is collected cell recording, can obtain being collected the concentration information of cell, reach the object of absolute counting.This scheme need to need user additionally accurately to allot the concentration of standard particle by the method for concentration known standard particulate inverse sample volume, higher to customer requirements, uses inconvenience, and has increased user's use cost.And conventional absolute volume metering method need to adopt fixed displacement pump to push away soon sample, because each quantitative volume is limited, when once pushing away sample and can not satisfy the demand, fixed displacement pump pushes away sample after need to again inhaling sample again, lose time, cause sample to occur measuring discontinuous problem in measuring, and fixed quantity circulation measure exist sample waste rate high, measure inefficient shortcoming.
Summary of the invention
The invention provides a kind of streaming instrument and liquid-way system thereof, method, realizing under the prerequisite of accurate quantification sample volume, reach the object of test constantly sample.
According to a first aspect of the invention, provide a kind of liquid-way system of streaming instrument, comprising:
Flow chamber, is sweeping along sample flow to pass through for holding sheath fluid, and provides the place being irradiated by light for sample flow;
Sample generator, for drawing sample from tested sample pipe;
Sheath fluid generator, its pipeline connection is to flow chamber, for sheath fluid is transported to flow chamber;
Waste liquid absorption plant, its pipeline connection is to flow chamber, for receiving the waste liquid flowing out from flow chamber;
N proportioning device, the pipeline of each proportioning device is communicated to respectively flow chamber and sample generator, described N is more than or equal to 2, each proportioning device is drawn quantitative sample liquid according to setting sequential from sample generator, and has at least a proportioning device that sample is transported to flow chamber according to the predetermined sample amount that pushes away in each period.
According to a second aspect of the invention, a kind of streaming instrument is provided, comprise liquid-way system, Systems for optical inspection and controller, described liquid-way system is for providing tested sample stream and collecting the sample flow waste liquid after detecting, described Systems for optical inspection is for providing the light beam that irradiates sample flow, and obtain the information of sample flow after light beam irradiates, described liquid-way system comprises:
Flow chamber, is sweeping along sample flow to pass through for holding sheath fluid, and provides the place being irradiated by light for sample flow;
Sample generator, for drawing sample from tested sample pipe;
Sheath fluid generator, its pipeline connection is to flow chamber, for sheath fluid is transported to flow chamber;
Waste liquid absorption plant, its pipeline connection is to flow chamber, for receiving the waste liquid flowing out from flow chamber;
N proportioning device, the pipeline of each proportioning device is communicated to respectively flow chamber and sample generator, and described N is more than or equal to 2;
Described controller is connected with proportioning device signal respectively, controls each proportioning device and from sample generator, draws quantitative sample liquid according to setting sequential, and have at least a proportioning device that sample is transported to flow chamber according to the predetermined sample amount that pushes away in each period.
According to a third aspect of the invention we, a kind of method that adopts streaming instrument to carry out particle analysis is provided, described streaming instrument comprises liquid-way system, Systems for optical inspection and controller, described liquid-way system is for providing tested sample stream and collecting the sample flow waste liquid after detecting, described Systems for optical inspection is for providing the light beam that irradiates sample flow, and obtain the information of sample flow after light beam irradiates, described liquid-way system comprises N proportioning device, described N is more than or equal to 2, described method comprises: control each proportioning device and from sample generator, draw quantitative sample liquid according to setting sequential, and have at least a proportioning device that sample is transported to flow chamber according to the predetermined sample amount that pushes away in each period.
The liquid-way system that the application provides, due to its proportioning device have a plurality of, therefore for combining of proportioning device inhaled sample and push away that sample provides may.
The streaming instrument that the application provides and particle analysis method are combined and are inhaled sample and push away sample by two or more proportioning devices, make each period have at least a proportioning device to flow chamber, to push away sample according to the predetermined sample amount that pushes away, therefore can constantly sample be sent in flow chamber, owing to pushing away sample amount, preset, therefore volume that can accurate quantification tested sample, realize accurate metering, and avoided the interruption in test process, realize the test constantly to sample, shortened the pattern detection time.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of embodiment of the application;
Fig. 2 is the structural representation of the another kind of embodiment of the application.
Embodiment
Below by embodiment, by reference to the accompanying drawings the present invention is described in further detail.
First the term of using in the application is made an explanation.
Absolute counting: refer to the detection that to be collected the concentration information of cell and to carry out in order to obtain, need the sample volume that obtains being collected the number of cell and detect use in this detection.
Push away soon: typically refer to quantitative portion device and fast sample is shifted onto in flow chamber, to wash away, take at ducted liquid, for example dilution or the sample that is diluted.Push away soon needed sample size and be called fast pushing volume.
Push away sample amount: be that specified amount portion device is pushed to the sample size in flow chamber when pushing away sample.
In the embodiment of the present application, streaming instrument uses two or more proportioning devices to combine and pushes away sample, make each proportioning device from sample generator, draw quantitative sample liquid according to setting sequential, and have at least a proportioning device that sample is transported to flow chamber according to the predetermined sample amount that pushes away in each period, if sample optics is divided into detection time, somely pushes away the sample period and inhale the sample period, for the technical scheme of only having a proportioning device, its control mode is that proportioning device is being inhaled sample period absorption sample, after inhaling sample and completing, to flow chamber, push away sample pushing away the sample period, after pushing away sample and completing, enter again and inhale the sample period and draw sample, and the control mode of a plurality of proportioning devices is no matter which period to have proportioning device that sample is pushed away to sample according to the predetermined sample amount that pushes away to flow chamber in the embodiment of the present application, be in flow chamber, constantly to have sample flow to flow through, and the sample volume that flows through flow chamber is the summation that each time pushes away sample amount.Thereby when realizing accurate quantification sample volume, reach the object of test constantly sample.This streaming instrument comprises liquid-way system, Systems for optical inspection and controller, liquid-way system is for providing the sheath fluid of tested sample stream and parcel sample flow and collecting the waste liquid after detecting, Systems for optical inspection is for providing the light beam that irradiates sample flow, and obtaining the information of sample flow after light beam irradiates, liquid-way system comprises flow chamber, sample generator, sheath fluid generator, waste liquid absorption plant and at least two proportioning devices.Flow chamber is used for holding sheath fluid and is sweeping along sample flow to pass through, and provides the place being irradiated by light for sample flow; Sample generator is for drawing sample from tested sample pipe; The pipeline connection of sheath fluid generator is to flow chamber, for sheath fluid is transported to flow chamber; The pipeline connection of waste liquid absorption plant is to flow chamber, for receiving the waste liquid flowing out from flow chamber; The pipeline of N proportioning device is all communicated to respectively flow chamber and sample generator, and N is more than or equal to 2.Controller is connected with N proportioning device signal respectively, and signal connects and comprises that wired connection is connected with radio communication.Controller is used for controlling each proportioning device and from sample generator, draws quantitative sample liquid according to setting sequential, and have at least a proportioning device that sample is transported to flow chamber according to the predetermined sample amount that pushes away in each period, for example in one embodiment, controller can be controlled N proportioning device and continue successively sample is transported to flow chamber according to the predetermined sample amount that pushes away, after a proportioning device pushes away sample and completes, and then control next proportioning device and push away sample, circulation constantly has a sample flow flow chamber successively, until detect, finishes.Systems for optical inspection is arranged near flow chamber conventionally, Systems for optical inspection forms light beam on the one hand, and make light beam irradiates flow through the sample flow in flow chamber, on the other hand, Systems for optical inspection is surveyed the information of sample flow after light beam irradiates, obtain forward signal, side direction signal and the fluorescence signal of sample, then by back end signal, process, output net result.
Owing to being provided with a plurality of proportioning devices, a plurality of proportioning devices can be inhaled in turn sample and push away sample, be to have a proportioning device to carry sample to flow chamber each period, so the sample flow in can flow chamber is carried out follow-on test, avoided again drawing sample because of wait proportioning device and caused test to be interrupted, so can reduce the pattern detection time.And owing to flowing to the sample flow of flow chamber, do not interrupt, so only need to once push away soon sample when detecting beginning, in the middle of test, do not need to push away soon sample, therefore reduced and pushed away soon the sample waste causing, improved sample utilization factor.
A plurality of proportioning devices can adopt the mode of serial or parallel connection to be arranged on the pipeline between flow chamber and sample generator.For controlling the flow direction of sample flow, when proportioning device is connected on the pipeline between flow chamber and sample generator, between two proportioning devices, and be provided with sample flow operation valve between proportioning device and sample generator, controller is controlled opening or closing of sample flow operation valve, when controller is controlled certain proportioning device to flow chamber conveying sample, control this proportioning device opens to the sample flow operation valve between flow chamber simultaneously, controlling this proportioning device closes to first sample flow operation valve on sample generator path, to reach, make sample flow flow to smoothly flow chamber, prevent the object of sample flow reversed flow, do not hinder again this proportioning device to draw sample to other proportioning devices on sample generator path simultaneously.When proportioning device is connected in parallel on the pipeline between flow chamber and sample generator, between proportioning device and flow chamber and between proportioning device and sample generator, sample flow operation valve is being set, sample flow operation valve is controlled by controller, can switch opening or closing between state.When controller is controlled certain proportioning device to flow chamber conveying sample, the sample flow operation valve of controlling between this proportioning device and flow chamber is opened, the sample flow operation valve of simultaneously controlling between this proportioning device and sample generator is closed so that sample can reverse direction flow to sample generator; When controller is controlled certain proportioning device from sample generator absorption sample, the sample flow operation valve of controlling between this proportioning device and flow chamber is closed, the sample flow operation valve of simultaneously controlling between this proportioning device and sample generator is opened, make sample flow only from sample generator, flow to proportioning device, prevent that sample flow from flowing to flow chamber.
Proportioning device for example can adopt fixed displacement pump, syringe or adopt other schemes to obtain the volume of samples, for example sample tube liquid level detection scheme, sample tube weigh scheme, by the capacitance variations conversion volume change of induction sample tube, by induction sample tube electromagnetic change conversion volume change etc.
Proportioning device can be two, three or four, and the proportioning device of take below describes as example as two.
Embodiment mono-:
Please refer to Fig. 1, waste liquid absorption plant comprises for holding waste liquid pool 110 and first operation valve 109 of the waste liquid flowing out from flow chamber, the first operation valve 109 is arranged on waste liquid pool 110 to the pipeline between flow chamber 108 outlet, and controlled switches opening or closing between state; Sheath fluid generator comprises for holding liquid storage tank 106 and second operation valve 107, the second operation valves 107 of sheath fluid and is arranged on liquid storage tank 106 to the pipeline between flow chamber 108 entrances, and controlled switches opening or closing between state.Proportioning device comprises the first proportioning device 103 and the second proportioning device 105, and sample generator is sampling needle 112, and sampling needle 112 can insert in tested sample pipe 101 and draw sample.The first proportioning device 103 and the second proportioning device 105 are arranged on the pipeline between flow chamber 108 and sampling needle 112 in the mode of series connection, the first proportioning device 103 is the proportioning device near sampling needle 112, the second proportioning device 105 is the proportioning device near flow chamber 108, on the pipeline between the first proportioning device 103 and sampling needle 112, be provided with the first sample flow operation valve 102, what the first sample flow operation valve 102 was controlled switches opening or closing between state, on the pipeline between the first proportioning device 103 and the second proportioning device 105, be provided with the second sample flow operation valve 104, what the second sample flow operation valve 104 was controlled switches opening or closing between state.Systems for optical inspection 111 is arranged on the both sides that flow chamber 108 is relative conventionally, for launching light beam and receiving the light signal after sample effect.
Various operation valves in above-described embodiment can be selected as required, for example, select solenoid valve, and controller (not shown) is connected with proportioning device with each operation valve respectively, control the switching over of solenoid valve and the suction sample of proportioning device and push away sample operation.
When the streaming instrument in employing the present embodiment is measured sample, its flow process can be as follows:
1, by inhaling sample and fast push operation, the dilution of taking in from sampling needle 112 to pipeline flow chamber 108 is discharged, to avoid the sample of subsequent delivery to be diluted liquid dilution.Can adopt the first proportioning device 103 to complete and inhale sample and push away soon, also can adopt the second proportioning device 105 to complete and inhale sample and push away soon, also can adopt the first proportioning device 103 and the second proportioning device 105 to coordinate and inhale sample and push away soon.
2, control two proportioning devices and alternately sample is transported to flow chamber according to the predetermined sample amount that pushes away.
Can be that first the first proportioning device 103 pushes away sample, can be also that first the second proportioning device 105 pushes away sample.
Because the first proportioning device 103 and the second proportioning device 105 mode with series connection is communicated with on the pipeline between flow chamber 108 and sampling needle 112, therefore, the second proportioning device 105 can be transported to ducted sample and inhale sample from the first proportioning device 103 in the first proportioning device 103 pushes away the process of sample to flow chamber 108, and the suction sample of the second proportioning device 105 pushes away sample at the first proportioning device 103 and completes before finishing, the sample size that the first proportioning device 103 is drawn equals the integral multiple of the sample size sum that sample size that the first proportioning device 103 carries to flow chamber 108 and the second proportioning device 105 draw from the sample flow of the first proportioning device 103 releases, and being more than or equal to the second proportioning device, the sample speed that pushes away of the first proportioning device pushes away 2 times of sample speed, to guarantee the flow through sample size of flow chamber 108 and the sample size that the second proportioning device 105 is drawn.
For example, suppose that the flow through sample size of flow chamber 108 of the first proportioning device 103 is V when flow chamber pushes away sample, it is also V with the sample size that pushes away sample that the second proportioning device 105 is inhaled sample, the each sample size of drawing of the first proportioning device 103 can be the integral multiple of 2V, can be 2V, 4V or 6V etc., the sample amount that pushes away that it is each is 2V, the each sample size of drawing of the first proportioning device 103 is different, its frequency of inhaling sample also can be different, when the each sample size of drawing of the first proportioning device 103 is 2V, its action for push away sample once, suction sample once; When the each sample size of drawing of the first proportioning device 103 is 4V, its action for push away sample twice, suction sample once; When the each sample size of drawing of the first proportioning device 103 is 6V, its action for push away sample three times, suction sample once, by that analogy.
For the testing process of streaming instrument clearer, that explain the present embodiment to sample, with a kind of concrete sample flow process that alternately pushes away, describe below, comprise the following steps:
1) inhale sample flow process: while inhaling sample, the first sample flow operation valve 102 and the second sample flow operation valve 104 are opened; The second proportioning device 105 is inhaled sample, and the sample size of absorption can make sample step the second sample flow operation valve 104, and when object makes follow-up quantitative the first proportioning device 103 inhale sample, sample can not be diluted.Then the second sample flow operation valve 104 is closed, the first proportioning device 103 is inhaled sample, the sample size that the first proportioning device 103 is drawn is: the liquid storage amount sum of pipeline between 3V, fast pushing volume and the second sample flow operation valve 104 and flow chamber, wherein V is the each sample amount that pushes away of the second proportioning device 105.In other specific embodiments, according to different control, the first suction amount of the first proportioning device 103 can be also other value, for example: the liquid storage amount sum of pipeline between 2V, fast pushing volume and the second sample flow operation valve 104 and flow chamber, or the liquid storage amount sum of pipeline between 4V, fast pushing volume and the second sample flow operation valve 104 and flow chamber.In the time of the first proportioning device 103 imbibition, the second proportioning device 105 resets.
2) fast plug-flow journey: after the first proportioning device 103 suction samples complete, the first sample flow operation valve 102 is closed, and the second sample flow operation valve 104 is opened; The first proportioning device 103 pushes away sample, makes sample arrive rapidly flow chamber, and after having pushed away soon, the first proportioning device 103 also retains the sample size of 2V.When pushing away soon, the second proportioning device 105 is inhaled sample, and suction amount is V.The second proportioning device 105 is inhaled sample and must before the first proportioning device 103 has pushed away soon, be finished.
3) measurement procedure: the first proportioning device 103 completes soon and postpones, the second sample flow operation valve 104 is closed, and the second proportioning device 105 pushes away sample by the flow of setting, and pushing away sample amount is V.The second proportioning device 105 completes and pushes away after sample, and the second sample flow operation valve 104 is opened, and the first proportioning device 103 pushes away sample to double the speed of the second proportioning device 105, pushing away sample amount is 2V, meanwhile, the speed that the second proportioning device 105 pushes away sample with it is inhaled sample, and suction amount is V.While making the first proportioning device 103 push away sample, enter the sample flow of flow chamber, consistent with the second proportioning device 105.The first proportioning device 103 pushes away sample complete after, the second sample flow operation valve 104 is closed, the second proportioning device 105 then pushes away sample with the speed of setting, pushing away sample amount is V, the first proportioning device 103 is inhaled samples simultaneously, suction amount is 2V, with this, circulates, until measure, finishes.Sample enters and under the parcel of sheath fluid, flows out flow chamber after flow chamber and be finally collected in waste liquid pool 10.The sample flow information of optical system 11 detection flows via flow chambers, forward signal, side direction signal and the fluorescence signal of acquisition sample, process, calculate output net result by back end signal.
Embodiment bis-:
Please refer to Fig. 2, waste liquid absorption plant comprises for holding waste liquid pool 210 and first operation valve 209 of the waste liquid flowing out from flow chamber, the first operation valve 209 is arranged on waste liquid pool 210 to the pipeline between flow chamber 208 outlet, and controlled switches opening or closing between state; Sheath fluid generator comprises for holding liquid storage tank 206 and second operation valve 207, the second operation valves 207 of sheath fluid and is arranged on liquid storage tank 206 to the pipeline between flow chamber 208 entrances, and controlled switches opening or closing between state.Proportioning device comprises the first proportioning device 203 and the second proportioning device 205, and sample generator is sampling needle 212, and sampling needle 212 can insert in tested sample pipe 201 and draw sample.The first proportioning device 203 and the second proportioning device 205 are arranged on the pipeline between flow chamber 208 and sampling needle 212 in parallel, on the pipeline between the first proportioning device 203 and sampling needle 212, be provided with the first sample flow operation valve 2021, on the pipeline between the first proportioning device 203 and flow chamber 208, be provided with the 3rd sample flow operation valve 2022, the first sample flow operation valve 2021, what the 3rd sample flow operation valve 2022 was controlled switches opening or closing between state, on the pipeline between the second proportioning device 205 and sampling needle 212, be provided with the second sample flow operation valve 2041, on the pipeline between the second proportioning device 205 and flow chamber 208, be provided with the 4th sample flow operation valve 2042, the second sample flow operation valve 2041, what the 4th sample flow operation valve 2042 was controlled switches opening or closing between state.Systems for optical inspection 211 is arranged on the both sides that flow chamber 208 is relative conventionally, for launching light beam and receiving the light signal after sample effect.
Because the first proportioning device 203 and the second proportioning device 205 are communicated with in parallel on the pipeline between flow chamber 208 and sampling needle 212, therefore, the first proportioning device 203 and the second proportioning device 205 can be inhaled sample by sampling needle 212 respectively from tested sample pipe 201, and then alternately to flow chamber 208, push away sample, make to keep having sample flow to flow through in flow chamber always.Can be that first the first proportioning device 203 pushes away sample, can be also that first the second proportioning device 105 pushes away sample.For example, two proportioning devices hocket and inhale sample and push away sample in complementary mode, and when the first proportioning device 203 is inhaled sample, the second proportioning device 205 pushes away sample to flow chamber 208, and when the second proportioning device 205 is inhaled sample, the first proportioning device 203 pushes away sample to flow chamber 208.The suction amount that the first proportioning device 203 is each and the each suction amount of the second proportioning device 205 can equate, also can not wait; The sample amount that pushes away that the first proportioning device 203 is each and the second proportioning device 205 are each pushes away sample amount and can equate, also can not wait.The each suction amount of two proportioning devices of take below and push away sample amount and all equate as example, illustrate a kind of idiographic flow that the streaming instrument in employing the present embodiment is measured sample.
1) inhale sample flow process: while inhaling sample, the first sample flow operation valve 2021, the second sample flow operation valve 2041 are opened; The 3rd sample flow operation valve 2022, the 4th sample flow operation valve 2042 are closed, the first proportioning device 203 and the second proportioning device 205 are inhaled sample, the sample size of drawing can make sample enter proportioning device, object is to make sample be full of respectively the pipeline between sample tube 201 to first proportioning devices 203 and the second proportioning device 205, so that the dilution in pipeline is drained, while making follow-up suction sample, sample can not be diluted.Then the first sample flow operation valve 2021 and the second sample flow operation valve 2041 are closed, and the 3rd sample flow operation valve 2022, the 4th sample flow operation valve 2042 and the first operation valve 209 are opened, and the first proportioning device 203 and the second proportioning device 205 reset.Then the 3rd sample flow operation valve 2022 and the 4th sample flow operation valve 2042 are closed, the first sample flow operation valve 2021 and the second sample flow operation valve 2041 are opened, the first proportioning device 203 and the second proportioning device 205 are inhaled samples, and institute inhales sample size and can support to push away soon, and measurement for the first time.
2) fast plug-flow journey: after the first proportioning device 203 and the second proportioning device 205 suction samples complete, the first sample flow operation valve 2021 and the second sample flow operation valve 2041 are closed, and the 3rd sample flow operation valve 2022 and the 4th sample flow operation valve 2042 are opened; The first proportioning device 203 and the second proportioning device 205 push away sample soon, make sample be full of pipeline and arrive rapidly flow chamber.Meanwhile, the second operation valve 207 is opened, and makes the sheath fluid in liquid storage tank 206 enter flow chamber.The first proportioning device 203 and the second proportioning device 205 can complete soon and push away simultaneously, also can successively complete soon and push away.
3) measurement procedure: the first proportioning device 203 and the second proportioning device 205 complete soon and postpone, one of them (for example first proportioning device 203) stops pushing away sample, another (for example second proportioning device 205) pushes away sample by given flow velocity, the second proportioning device 205 completes and pushes away after sample, and the first proportioning device 203 then pushes away sample with same speed.Then the 4th sample flow operation valve 2042 is closed, and the second sample flow operation valve 2041 is opened, and the second proportioning device 205 is inhaled samples, and the second proportioning device 205 is inhaled samples and must be completed before pushing away sample and finish at the first proportioning device 203.The first proportioning device 203 completes and pushes away after sample, and the second proportioning device 205 then pushes away sample with same speed.Then the 3rd sample flow operation valve 2022 is closed, and the first sample flow operation valve 2021 is opened, and the first proportioning device 203 is inhaled samples, and the first proportioning device 203 is inhaled samples and must be completed before pushing away sample and finish at the second proportioning device 205.With this, circulate, until measure, finish.Sample enters and under the parcel of sheath fluid, flows out flow chamber after flow chamber and be finally collected in waste liquid pool 210.The sample flow information of Systems for optical inspection 211 detection flows via flow chambers, forward signal, side direction signal and the fluorescence signal of acquisition sample, process, calculate output net result by back end signal.
In above-described embodiment, by combining of two or more proportioning devices inhaling sample and push away sample, the sample flow that enters flow chamber while making each proportioning device push away sample is consistent, and each to push away liquid measure also consistent, and can constantly sample be sent in flow chamber.Volume that so not only can accurate quantification tested sample, realizes accurate metering, and can realize test constantly.
According to the disclosed content of the application, it will be appreciated by those skilled in the art that waste liquid absorption plant and sheath fluid generator also can adopt other scheme existing or that occur in the future to realize.For example waste liquid absorption plant can also comprise the vacuum extractor of waste liquid pool and controllable switch, when needs receive waste liquid, opens vacuum plant, makes waste liquid pool form subnormal ambient, makes waste liquid flow into waste liquid pool.Sheath fluid generator also can be realized by the active transportation device of controllable switch, when needs are exported sheath fluid, opens switch, when not needing to export sheath fluid, and closing switch.
Above content is in conjunction with concrete embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (21)
1. a liquid-way system for streaming instrument, is characterized in that comprising:
Flow chamber, is sweeping along sample flow to pass through for holding sheath fluid, and provides the place being irradiated by light for sample flow;
Sample generator, for drawing sample from tested sample pipe;
Sheath fluid generator, its pipeline connection is to flow chamber, for sheath fluid is transported to flow chamber;
Waste liquid absorption plant, its pipeline connection is to flow chamber, for receiving the waste liquid flowing out from flow chamber;
N proportioning device, the pipeline of each proportioning device is communicated to respectively flow chamber and sample generator, and described N is more than or equal to 2.
2. liquid-way system as claimed in claim 1, is characterized in that, described proportioning device is connected on the pipeline between flow chamber and sample generator.
3. liquid-way system as claimed in claim 2, is characterized in that, between two proportioning devices and between proportioning device and sample generator, is being provided with sample flow operation valve, and what sample flow operation valve was controlled switches opening or closing between state.
4. liquid-way system as claimed in claim 1, is characterized in that, described proportioning device is connected in parallel on the pipeline between flow chamber and sample generator.
5. liquid-way system as claimed in claim 4, is characterized in that, between proportioning device and flow chamber and between proportioning device and sample generator, is being provided with sample flow operation valve, and what sample flow operation valve was controlled switches opening or closing between state.
6. the liquid-way system as described in any one in claim 1-5, it is characterized in that, waste liquid absorption plant comprises for holding waste liquid pool and first operation valve of the waste liquid flowing out from flow chamber, described the first operation valve is arranged on waste liquid pool to the pipeline between flow chamber outlet, and controlled switches opening or closing between state; Sheath fluid generator comprises that described the second operation valve is arranged on liquid storage tank to the pipeline between flow chamber entrance for holding liquid storage tank and second operation valve of sheath fluid, and controlled switches opening or closing between state.
7. a streaming instrument, comprise liquid-way system, Systems for optical inspection and controller, described liquid-way system is for providing tested sample stream and collecting the sample flow waste liquid after detecting, described Systems for optical inspection is for providing the light beam that irradiates sample flow, and obtain the information of sample flow after light beam irradiates, it is characterized in that, described liquid-way system comprises:
Flow chamber, is sweeping along sample flow to pass through for holding sheath fluid, and provides the place being irradiated by light for sample flow;
Sample generator, for drawing sample from tested sample pipe;
Sheath fluid generator, its pipeline connection is to flow chamber, for sheath fluid is transported to flow chamber;
Waste liquid absorption plant, its pipeline connection is to flow chamber, for receiving the waste liquid flowing out from flow chamber;
N proportioning device, the pipeline of each proportioning device is communicated to respectively flow chamber and sample generator, and described N is more than or equal to 2;
Described controller is connected with proportioning device signal respectively, controls each proportioning device and from sample generator, draws quantitative sample liquid according to setting sequential, and have at least a proportioning device that sample is transported to flow chamber according to the predetermined sample amount that pushes away in each period.
8. streaming instrument as claimed in claim 7, is characterized in that, N proportioning device of described controller control continues successively sample is transported to flow chamber according to the predetermined sample amount that pushes away.
9. streaming instrument as claimed in claim 7 or 8, is characterized in that, described proportioning device is connected on the pipeline between flow chamber and sample generator.
10. streaming instrument as claimed in claim 9, is characterized in that, between adjacent two proportioning devices and between proportioning device and sample generator, is being provided with sample flow operation valve, and what sample flow operation valve was controlled switches opening or closing between state.
11. streaming instruments as claimed in claim 10, it is characterized in that, when described controller is controlled X proportioning device to flow chamber conveying sample, control this X proportioning device opens to the sample flow operation valve between flow chamber simultaneously, control this X proportioning device and close to first sample flow operation valve on sample generator path, described X proportioning device is any in N proportioning device.
12. streaming instruments as claimed in claim 9, it is characterized in that, described proportioning device is two, described controller is controlled two proportioning devices and alternately sample is transported to flow chamber according to the predetermined sample amount that pushes away, when the first proportioning device of the close sample generator of described controller control pushes away sample, the sample flow of releasing from the first proportioning device away from the second proportioning device of sample generator, inhale sample, and the suction sample of the second proportioning device pushes away sample at the first proportioning device and completes before finishing.
13. streaming instruments as claimed in claim 12, it is characterized in that, the sample speed that pushes away of described the first proportioning device is more than or equal to the second proportioning device and pushes away 2 times of sample speed, and the sample size that described the first proportioning device is drawn from sample generator equals the integral multiple of the sample size sum that sample size that the first proportioning device carries to flow chamber and the second proportioning device draw from the sample flow of the first proportioning device release.
14. streaming instruments as claimed in claim 7 or 8, is characterized in that, described proportioning device is connected in parallel on the pipeline between flow chamber and sample generator.
15. streaming instruments as claimed in claim 14, is characterized in that, between proportioning device and flow chamber and between proportioning device and sample generator, are being provided with sample flow operation valve, and what sample flow operation valve was controlled switches opening or closing between state.
16. streaming instruments as claimed in claim 15, it is characterized in that, when described controller is controlled Y proportioning device to flow chamber conveying sample, the sample flow operation valve of controlling between this Y proportioning device and flow chamber is opened, and the sample flow operation valve between this Y proportioning device and sample generator is closed simultaneously; When described controller is controlled certain proportioning device from sample generator absorption sample, the sample flow operation valve of controlling between this proportioning device and flow chamber is closed, sample flow operation valve between this proportioning device and sample generator is opened simultaneously, and described Y proportioning device is any in N proportioning device.
17. streaming instruments as claimed in claim 16, is characterized in that, described proportioning device is two, and described controller is controlled two proportioning devices and hocketed and inhale sample and push away sample in complementary mode.
18. 1 kinds of methods that adopt streaming instrument to carry out particle analysis, described streaming instrument comprises liquid-way system, Systems for optical inspection and controller, described liquid-way system is for providing tested sample stream and collecting the sample flow waste liquid after detecting, described Systems for optical inspection is for providing the light beam that irradiates sample flow, and obtain the information of sample flow after light beam irradiates, described liquid-way system comprises N proportioning device, described N is more than or equal to 2, it is characterized in that, described method comprises: control each proportioning device and from sample generator, draw quantitative sample liquid according to setting sequential, and have at least a proportioning device that sample is transported to flow chamber according to the predetermined sample amount that pushes away in each period.
19. methods as claimed in claim 18, it is characterized in that, described proportioning device is two, and described have at least a proportioning device that sample is transported to flow chamber according to the predetermined sample amount that pushes away in each period to comprise: control two proportioning devices and alternately sample is transported to flow chamber according to the predetermined sample amount that pushes away.
20. methods as claimed in claim 19, is characterized in that, two proportioning devices are connected on the pipeline between flow chamber and sample generator, control two proportioning devices and alternately sample are transported to flow chamber according to the predetermined sample amount that pushes away and comprise:
Control near the first proportioning device of sample generator and according to the first predetermined suction amount, inhale sample from sample generator;
Controlling the first proportioning device pushes the first suction amount of drawing to flow chamber, when the first proportioning device pushes away sample, control the sample flow of releasing from the first proportioning device away from the second proportioning device of sample generator simultaneously and inhale sample according to the second predetermined suction amount, and the suction sample of the second proportioning device pushes away sample at the first proportioning device and completes before finishing;
Controlling the second proportioning device pushes the second suction amount of drawing to flow chamber, control the first proportioning device simultaneously and according to the first predetermined suction amount, inhale sample from sample generator, and the suction sample of the first proportioning device pushes away sample at the second proportioning device and completes before finishing, according to this circulation;
Described the first suction amount equals 2 times of the second suction amount, and the sample speed that pushes away of the first proportioning device is 2 times that the second proportioning device pushes away sample speed.
21. methods as claimed in claim 19, it is characterized in that, two proportioning devices are connected in parallel on the pipeline between flow chamber and sample generator, and described have at least a proportioning device that sample is transported to flow chamber according to the predetermined sample amount that pushes away in each period to comprise: make two proportioning devices hocket and inhale sample and push away sample to flow chamber in complementary mode.
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