CN216350218U

CN216350218U - Electrical impedance counter and sample analyzer

Info

Publication number: CN216350218U
Application number: CN202122753858.3U
Authority: CN
Inventors: 褚聪; 刘俊龙; 池书锐; 甘小锋
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2021-11-09
Filing date: 2021-11-09
Publication date: 2022-04-19
Anticipated expiration: 2031-11-09

Abstract

The utility model discloses an electrical impedance counting device and a sample analyzer, wherein the electrical impedance counting device comprises a front pool, a rear pool, a counting hole communicated with the front pool and the rear pool, and a constant current source connected with the rear pool through a pipeline, the front pool is provided with a first electrode, the rear pool is provided with a second electrode, when a sample liquid to be detected enters the rear pool from the front pool through the counting hole, the electrical impedance between the first electrode and the second electrode is changed, the constant current source provides power to drive the sample liquid to pass through the counting hole at a constant speed, the quantity of the sample liquid passing through the counting hole in unit time is ensured to be constant, and therefore, the multiple detection of the same biological sample by the analyzer or the detection results of the same biological sample by different analyzers are consistent, and the accuracy of classification counting is ensured.

Description

Electrical impedance counter and sample analyzer

Technical Field

The utility model relates to the technical field of sample detection, in particular to an electrical impedance counting device and a sample analyzer.

Background

The change of human physiology and pathology usually causes the change of blood components, and the detection and analysis of blood cells in a blood sample, including White Blood Cells (WBC), Red Blood Cells (RBC), Platelets (PLT), Hemoglobin (HGB) and the like, can provide a basis for diagnosis and treatment of diseases.

The existing sample analyzer mainly performs the classification counting of blood cells by an electrical impedance method, a counting device of the sample analyzer is composed of a front pool and a rear pool which are communicated through a small hole, a sample liquid to be detected enters the rear pool from the front pool through the small hole under the action of a negative pressure source, and the electrical impedance between electrodes at two sides of the small hole is changed when the blood cells pass through the small hole, so that pulses are generated. The larger the volume of blood cells passing through the aperture, the greater the change in electrical impedance, and the higher the pulse amplitude produced. Blood cells can be classified by measuring the pulse size, various blood cells can be counted by recording the number of pulses of various sizes, and whether the blood cells are in a normal range or not is judged.

However, as the sample liquid flows, the magnitude of the negative pressure provided by the negative pressure source changes, which may cause the flow rate of the sample liquid to change, and affect the accuracy of the classification count.

Disclosure of Invention

In view of the above, an electrical impedance counting apparatus capable of accurately performing classification counting and a sample analyzer using the electrical impedance counting apparatus are provided.

The utility model provides an electrical impedance counting device which comprises a front pool, a rear pool, a counting hole and a constant current source, wherein the counting hole is communicated with the front pool and the rear pool, the constant current source is connected with the rear pool through a pipeline, the front pool is provided with a first electrode, the rear pool is provided with a second electrode, when sample liquid to be detected enters the rear pool from the front pool through the counting hole, the electrical impedance between the first electrode and the second electrode is changed, and the constant current source provides power to drive the sample liquid to pass through the counting hole at a constant speed.

Further, the constant current source is an injector, an air source, a quantitative pump, a peristaltic pump or an injection pump.

Furthermore, a pressure sensor is arranged on a pipeline between the constant current source and the rear pool and is connected with a controller, and the controller judges whether the counting hole is blocked according to a signal of the pressure sensor.

Further, the back pond is equipped with two at least first interfaces and second interface, the axis of two at least first interfaces for the axis slope setting of count hole, the second interface with count hole coaxial setting.

Furthermore, the first interface is connected with a cleaning liquid container through a first on-off valve and used for cleaning the rear pool and/or the counting hole, and the second interface is connected with the constant current source through a second on-off valve and used for counting cells.

Furthermore, the second interface is connected with a cleaning liquid container through a second on-off valve and used for cleaning the rear pool and/or the counting hole, and the first interface is connected with the constant current source through a first on-off valve and used for counting cells.

Further, a liquid outlet is formed in the bottom of the front pool, the liquid outlet is connected with the waste liquid tank through a liquid outlet pipeline, and a third cut-off valve is arranged on a liquid outlet connecting pipeline between the liquid outlet and the waste liquid tank.

The utility model also provides a sample analyzer, which comprises the electrical impedance counting device and a diluent supply device for supplying diluent to a forebay of the electrical impedance counting device, wherein a biological sample to be detected and the diluent are mixed in the forebay to form the sample liquid to be detected.

Further, the diluent supply device comprises a first pouring pipe arranged above the front pool, and diluent is filled through the first pouring pipe; alternatively, the diluent supply device may include a first reagent needle provided above the front pool, and the diluent may be supplied through the first reagent needle.

Further, the hemolytic agent supplying device is used for filling hemolytic agent into the front pool, and comprises a second liquid injection pipe arranged above the front pool, and the hemolytic agent is filled through the second liquid injection pipe; or the hemolytic agent supply device includes a second reagent needle disposed above the front pool, through which a hemolytic agent is filled.

Compared with the prior art, the constant-current source is adopted to drive the sample liquid to pass through the counting holes, the power provided by the constant-current source can be adjusted in real time according to the flow of the sample liquid, unstable factors caused by tolerance of the counting holes, pipeline flow resistance and the like are made up, the sample liquid can pass through the counting holes at a constant speed, the quantity of the sample liquid passing through the counting holes in unit time is ensured to be constant, and therefore the result of multiple detection of the same biological sample through an analyzer or the result of detection of the same biological sample through different analyzers are consistent, and the accuracy of classification counting is ensured.

Drawings

FIG. 1 is a schematic view of an electrical impedance counting apparatus of a sample analyzer according to an embodiment of the present invention.

Fig. 2 is a schematic view of another embodiment of the electrical impedance counting apparatus of the present invention.

FIG. 3 is a flow chart of the sample analyzer of the present invention for blood sample testing.

Detailed Description

To facilitate an understanding of the utility model, the utility model will now be described more fully with reference to the accompanying drawings. One or more embodiments of the present invention are illustrated in the accompanying drawings to provide a more accurate and thorough understanding of the disclosed embodiments. It should be understood, however, that the present invention may be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein.

The same or similar reference numbers in the drawings correspond to the same or similar parts; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", etc. based on the orientation or positional relationship shown in the drawings, it is only for convenience of describing the present invention and simplifying the description, but it is not intended to indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and are not to be construed as limiting the present patent, and the specific meaning of the terms may be understood by those skilled in the art according to specific circumstances.

The utility model provides a sample analyzer and an electrical impedance counting device thereof, which are used for detecting biological samples and counting and classifying various cells in the biological samples (such as blood samples and the like). Fig. 1 shows an embodiment of the electrical impedance counting apparatus of the present invention, which comprises a front cell 10, a rear cell 20 and a constant current source 30.

A counting hole 40 is arranged between the front tank 10 and the rear tank 20, and the counting hole 40 communicates the front tank 10 with the rear tank 20; the constant current source 30 may be a power source such as an injector, an air source, a constant displacement pump, a peristaltic pump or an injection pump, and is connected to the rear pool 20 through a pipeline. The biological sample to be measured is mixed in the electrolyte solution to form a sample liquid, and the sample liquid flows from the front pool 10 to the rear pool 20 through the counting hole 40 under the action of the constant current source 30, so that the amount of the sample liquid passing through the counting hole in unit time is fixed. The front cell 10 is provided with a first electrode, the rear cell 20 is provided with a second electrode, and the first electrode and the second electrode are respectively positioned at two opposite sides of the counting hole 40 and are conducted through the sample liquid. Generally, a power supply is connected between the first electrode and the second electrode, and a stable and unchanging current is established between the first electrode and the second electrode after the power supply is started. Since the conductivity of each cell in the sample liquid is generally lower than that of the electrolyte solution, when the cell passes through the counting hole 40, the electrical impedance between the first electrode and the second electrode increases, a voltage change is instantaneously caused to generate a pulse, and the larger the volume of the cell is, the larger the change in the electrical impedance is caused to be, and the higher the amplitude of the generated pulse is.

The first electrode and the second electrode are connected with a counting circuit, pulse signals generated when various cells in the sample liquid pass through the counting hole 40 are acquired by the counting circuit, the counting circuit classifies the cells according to the difference of the amplitude of the pulse signals, and counts the number of various pulses with the same amplitude respectively to realize the counting of various cells, thereby realizing the classification of various cells in the sample liquid. According to the utility model, the constant current source 30 is adopted to drive the sample liquid to pass through the counting hole 40, and the power provided by the constant current source 30 can be adjusted in real time according to the flow of the sample liquid, so that unstable factors caused by tolerance of the counting hole 40, pipeline flow resistance and the like are compensated, the sample liquid can pass through the counting hole 40 at a constant speed, the quantity of the sample liquid passing through the counting hole 40 in unit time is ensured to be constant, and thus the results of multiple detections of the same biological sample by an analyzer or the detections of the same biological sample by different analyzers are consistent, and the accuracy of classification counting is ensured.

As shown in fig. 2, in an embodiment, the rear pool 20 is provided with at least two first ports 22 and a second port 24, the axes of the two first ports 22 are obliquely arranged relative to the axis of the counting hole 40, and the second port 24 is coaxially arranged with the counting hole 40. The first port 22 is connected to the cleaning liquid container 70 through the first on-off valve 50 for cleaning the rear reservoir 20 and/or the counting well 40; the second interface 24 is connected via a second shut-off valve 52 to a constant current source 30 for cell counting. In other embodiments, the second port 24 may also be connected to the cleaning solution container 70 via the second on-off valve 52 for cleaning the wash reservoir 20 and/or the counting well 40, and the first port 22 is connected to the constant current source 30 via the first on-off valve 50 for cell counting.

By the negative pressure provided by the stable constant current source 30, the sample liquid enters the rear cell 20 from the front cell 10 through the counting hole 40, and the electrical impedance counting of the cells is performed. Preferably, the second on-off valve 52 may be an electromagnetic valve, etc., and the second on-off valve 52 may control the on-off of the pipeline between the rear pool 20 and the constant current source 30, so that during the pretreatment of the biological sample to be tested, such as dilution treatment and hemolysis treatment, the second on-off valve 52 is kept in a closed state, and the pipeline between the rear pool 20 and the constant current source 30 is disconnected. As shown in fig. 2, a pressure sensor 60 may be further disposed on the pipeline between the rear pool 20 and the constant current source 30, the pressure sensor 60 is connected with a controller, an alarm, and the like to form a control loop, the controller determines that the counting hole 40 is sufficiently blocked according to the pipeline pressure fed back by the pressure sensor 60, and if the pressure exceeds a threshold value, the counting hole 40 is blocked, the alarm is started to prompt a user to perform a cleaning sequence of the counting hole 40.

The forebay 10 can be used as a reaction bay for pretreatment of a biological sample to be tested, for example, in the detection of a blood sample, since the existing detection method cannot accurately detect high-concentration particles in the blood sample, and the blood sample needs to be diluted by hundreds of times to be within a linear range of a standard curve, a diluent such as EPK, DIL and the like can be added in the forebay 10 in advance through a diluent supply device, and then the blood sample is injected into the forebay 10 to be mixed with the diluent. The diluent is generally electrically conductive and may be used as an electrolyte solution. The diluent supply device includes a diluent container 72 and a first pouring tube 80 provided above the front tank 10, and the first pouring tube 80 is connected to the diluent container 72 to fill the front tank 10 with the diluent through the first pouring tube 80. In other embodiments, the first dispensing tube 80 can be replaced with a first reagent needle disposed above the opening of the forebasin 10 for filling with the diluent. In the illustration, a third shut-off valve 54, such as a solenoid valve, is provided on the delivery line between the diluent container 72 and the first pouring tube 80 to control the opening and closing of the delivery line. In some embodiments, the diluent container 72 may also be in direct communication with the forebay 10 via a transfer line, and the first reagent needle may be omitted. The diluted blood sample passes through the counting hole 40, and the RBC and PLT can be classified and counted.

In some embodiments, the pre-treatment of the biological sample to be tested further comprises adding a hemolytic agent, such as red blood cell hemolytic agent SLS, leukocyte differential hemolytic agent FFD, etc., to the diluted sample for hemolytic treatment. Wherein, the hemolytic agent FFD can dissolve red blood cells, so as to be convenient for the classification and counting of white blood cells; the hemolytic agent SLS enables the red blood cells to release hemoglobin, and therefore hemoglobin counting is convenient. Similarly, the sample analyzer includes a hemolytic agent supply device for filling the front cell 10 with hemolytic agent, the hemolytic agent supply device includes a hemolytic agent container 74 and a second liquid injection tube 82 disposed above the front cell, the hemolytic agent container 74 is connected to the second liquid injection tube 82, and the front cell 10 is filled with hemolytic agent through the second liquid injection tube 82. Of course, in other embodiments, the second priming tube 82 may be replaced with a second reagent needle disposed above the forebay 10 through which the hemolytic agent is filled. In an embodiment where both the diluent and the hemolysing agent are added through a reagent needle, the first reagent needle and the second reagent needle may be one and the same.

In this embodiment, the first interface 22 is connected to the cleaning liquid container 70, and after the electrical impedance counting apparatus of the present invention is used for a certain period of time, the cleaning liquid is injected into the rear pool 20 through the first interface 22 for cleaning, and particularly the counting holes 40 are cleaned, so as to avoid the counting holes 40 from being blocked to influence the accuracy of the classification counting. Preferably, the plurality of first ports 22 are symmetrically arranged, such as the plurality of first ports 22 are symmetrically disposed about an axis of the second port 24. The axis of each first port 22 is inclined at an angle relative to the axis of the counting hole 40, so that the cleaning agent can be directly sprayed out towards the counting hole 40, and the cleaning effect is ensured. In addition, a liquid discharge port 14 is provided at the bottom of the front pool 10, and the liquid discharge port 14 is connected to the waste liquid tank 76 through a liquid discharge pipe to discharge the remaining sample liquid in the front pool 10 to the waste liquid tank 76 for temporary storage. Similarly, a fourth shut-off valve 56, such as a solenoid valve, is disposed on the drain line between the front pool 10 and the waste liquid tank 76 to control the on/off of the drain line.

Referring to fig. 3, the detection process of the sample analyzer of the present invention is as follows:

cleaning the front tank 10 and the rear tank 20;

diluting a biological sample to be detected in the forebay 10 according to a preset dilution ratio to form a sample liquid, wherein the second cut-off valve 52 is kept closed;

starting the constant current source 30 and opening the second cut-off valve 52, and driving the sample liquid to enter the rear pool 20 through the counting hole 40 at a constant speed under the constant negative pressure action of the constant current source 30; and

the cells of the sample fluid are counted and sorted according to the electrical impedance of the sample fluid through the counting well 40.

When the sample analyzer is applied to the detection of a blood sample, diluted sample liquid passes through the counting hole 40 under the action of the constant current source 30, so that the classified counting of RBC and PLT of the blood sample is realized; hemolytic agent is added into the sample liquid, and the sample liquid after hemolysis treatment passes through the counting hole 40 under the action of the constant current source 30, so that the five-classification counting of WBC of the blood sample is realized. In the detection of the blood sample, the classification counting of RBC and PLT and the five classification counting of WBC can be respectively detected by two samples, or can be sequentially detected by one sample, at this time, the sample liquid diluted by the pre-moving part passes through the counting hole 40 to perform the classification counting of RBC and PLT, and then hemolytic agent is added into the residual sample liquid to perform the five classification counting of WBC, so that the consumption of the blood sample is reduced.

It should be noted that the present invention is not limited to the above-mentioned embodiments, and other changes and modifications can be made by those skilled in the art according to the spirit of the present invention, and these changes and modifications made according to the spirit of the present invention should be included in the scope of the present invention as claimed.

Claims

1. The utility model provides an electrical impedance counting assembly, its characterized in that, including forebay, back pond, intercommunication the count hole of forebay and back pond and through the pipeline with the constant current source that the back pond is connected, the forebay be provided with first electrode the back pond is provided with the second electrode, and the sample liquid that awaits measuring passes through the count hole by the forebay gets into make during the back pond electrical impedance between first electrode and the second electrode produces the change, the constant current source provides power drive sample liquid at the uniform velocity and passes through the count hole.

2. The electrical impedance counting device of claim 1, wherein the constant current source is a syringe, a gas source, a dosing pump, a peristaltic pump, or a syringe pump.

3. The electrical impedance counting device of claim 2, wherein a pressure sensor is arranged on a pipeline between the constant current source and the rear pool, the pressure sensor is connected with a controller, and the controller judges whether the counting hole is blocked according to a signal of the pressure sensor.

4. The electrical impedance counting device of claim 1, wherein the back cell is provided with at least two first ports and a second port, the axes of the at least two first ports being arranged obliquely with respect to the axis of the counting hole, the second port being arranged coaxially with the counting hole.

5. An electrical impedance counting device according to claim 4, wherein the first interface is connected to a washing liquid container via a first on-off valve for washing the rear well and/or the counting well, and the second interface is connected to the constant current source via a second on-off valve for cell counting.

6. An electrical impedance counting device according to claim 4, wherein the second interface is connected to a washing liquid container via a second on-off valve for washing the rear reservoir and/or the counting well, and the first interface is connected to the constant current source via a first on-off valve for cell counting.

7. The electrical impedance counting device according to claim 1, wherein a liquid outlet is arranged at the bottom of the forebay, the liquid outlet is connected with the waste liquid tank through a liquid outlet pipeline, and a third cut-off valve is arranged on a liquid outlet connecting pipeline between the liquid outlet and the waste liquid tank.

8. A sample analyser comprising an electrical impedance counting device according to any one of claims 1 to 7 and diluent supply means for supplying a diluent to a forebay of the electrical impedance counting device, the biological sample to be measured and the diluent being mixed in the forebay to form the sample fluid to be measured.

9. The sample analyzer of claim 8 wherein the diluent supply means includes a first dispensing tube disposed above the forebay through which diluent is dispensed; alternatively, the diluent supply device may include a first reagent needle provided above the front pool, and the diluent may be supplied through the first reagent needle.

10. The sample analyzer of claim 8, further comprising a hemolytic agent supply device that fills the pre-chamber with a hemolytic agent, the hemolytic agent supply device including a second liquid injection tube provided above the pre-chamber through which the hemolytic agent is filled; or the hemolytic agent supply device includes a second reagent needle disposed above the front pool, through which a hemolytic agent is filled.