CN105203771A - Serum amyloid protein A determination kit and use method thereof - Google Patents
Serum amyloid protein A determination kit and use method thereof Download PDFInfo
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The invention discloses a serum amyloid protein A determination kit and a use method thereof. The serum amyloid protein A determination kit comprises first detection liquid and second detection liquid, wherein the first detection liquid includes first detection liquid Tris-HCl buffer liquid and first detection liquid sodium azide, and the second detection liquid includes second detection liquid Tris-HCl buffer liquid, second detection liquid sodium azide and latex particles coated with anti-human serum amyloid protein A antibodies. According to the serum amyloid protein A determination kit and the use method of the serum amyloid protein A determination kit, by adopting latex-enhanced immunoturbidimetry, the operation is simple, the accuracy is high, and the popularization is facilitated.
Description
Technical field
The present invention relates to a kind of serum amyloid A protein and measure kit and using method thereof.
Background technology
Serum amyloid A protein (serumamyloidAprotein, SAA) is acute phase protein, and during inflammation, infectivity and noninfectious disease, its concentration in blood sharply can raise within a few hours, can be elevated to 1000 times of initial concentration; SAA and HDL is relevant, and it can regulate the metabolism of HDL between inflammatory phase, and the characteristic of a SAA particular importance is that its catabolite can be deposited in different organs in the fibriilar mode of amyloid A, and in chronic inflammatory diseases, this is a kind of serious complication; Serum amyloid A protein raises and also sees atherosclerotic, diabetic nephropathy, acute myocardial infarction AMI, coronary heart disease, chronic renal disease.In assess inflammation, monitor in its activity and treatment, SAA and CRP has similar effect.SAA detects and occurs, in virus infections, the patient (particularly carrying out the patient of immunosuppressive therapy) of kidney transplantation exclusion reaction and the cystic fibrosis patient of adrenocortical hormones in treating, to detect more conclusive than c reactive protein in diagnosis.Research finds, in the arthritic case of trouble, the relation of serum amyloid A protein and Disease Activity is the closest.Detection c reactive protein and serum amyloid A protein can improve the diagnostic sensitivity to infecting simultaneously.But current used to measure its accuracy of kit about serum amyloid A protein low, and complicated operation, not easily promote.
Summary of the invention
The object of the present invention is to provide a kind of serum amyloid A protein to measure kit and using method thereof, it is by latex enhancing immune turbidimetry, and simple to operate, accuracy is high, is convenient to promote.
In order to achieve the above object, technical scheme of the present invention is:
A kind of serum amyloid A protein measures kit, comprise the first detection liquid, second and detect liquid, first detects liquid comprises the first detection liquid Tris-HCl damping fluid, the first detection liquid sodium azide, the composition of each component is respectively by weight percentage: first detects liquid Tris-HCl damping fluid 97-99%, the first detects liquid sodium azide 1-3%; Second detection liquid comprises the second detection liquid Tris-HCl damping fluid, second and detects liquid sodium azide, is coated with the latex particle of AHS's amyloid A antibody, the composition of each component is respectively by weight percentage: second detects liquid Tris-HCl damping fluid 94-98%, second detects liquid sodium azide 1-3%, is coated with the latex particle 1-3% of AHS's amyloid A antibody.
First detects liquid comprises the first detection liquid Tris-HCl damping fluid, the first detection liquid sodium azide, and the composition of each component is respectively by weight percentage: first detects liquid Tris-HCl damping fluid 98%, first detects liquid sodium azide 2%; Second detection liquid comprises the second detection liquid Tris-HCl damping fluid, second and detects liquid sodium azide, is coated with the latex particle of AHS's amyloid A antibody, the composition of each component is respectively by weight percentage: second detects liquid Tris-HCl damping fluid 96%, second detects liquid sodium azide 2%, is coated with the latex particle 2% of AHS's amyloid A antibody.
Serum amyloid A protein measures a using method for kit, comprises the steps: first to prepare the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube; Then in the first Reagent Tube, add the first detection liquid 250 μ L, distilled water 8 μ L mixes and hatches 5 minutes, in the second Reagent Tube, add the first detection liquid 250 μ L simultaneously, titer 8 μ L mixes and hatches 5 minutes, in the 3rd Reagent Tube, add the first detection liquid 250 μ L simultaneously, detect liquid 8 μ L and mix and hatch 5 minutes; Then in the first Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute, in the second Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute simultaneously, in the 3rd Reagent Tube, add the second detection liquid simultaneously add 50 μ L and mix and hatch 1 minute; Finally read the absorbance of the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube respectively, absorbance is compared with the titer of concentration known, thus draw the content detecting serum amyloid A protein in liquid.
Described temperature when hatching is 37 DEG C.
Be coated with the latex particle of serum amyloid A protein (SAA) antibody, when mixing with the detection liquid containing SAA antigen, agglutinating reaction can occur, thus cause the change of absorbance, its size is proportional with SAA antigenic content in detection liquid.Absorbance change is compared with the titer of concentration known, quantitatively can draw the content detecting serum amyloid A protein in liquid.
The invention has the beneficial effects as follows: a kind of serum amyloid A protein measures kit and using method thereof, it is by latex enhancing immune turbidimetry, and simple to operate, accuracy is high, is convenient to promote.
Embodiment
Embodiment 1
A kind of serum amyloid A protein measures kit, comprise the first detection liquid, second and detect liquid, first detects liquid comprises the first detection liquid Tris-HCl damping fluid, the first detection liquid sodium azide, the composition of each component is respectively by weight percentage: first detects liquid Tris-HCl damping fluid 98%, first detects liquid sodium azide 2%; Second detection liquid comprises the second detection liquid Tris-HCl damping fluid, second and detects liquid sodium azide, is coated with the latex particle of AHS's amyloid A antibody, the composition of each component is respectively by weight percentage: second detects liquid Tris-HCl damping fluid 96%, second detects liquid sodium azide 2%, is coated with the latex particle 2% of AHS's amyloid A antibody.
Serum amyloid A protein measures a using method for kit, comprises the steps: first to prepare the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube; Then in the first Reagent Tube, add the first detection liquid 250 μ L, distilled water 8 μ L mixes and hatches 5 minutes, in the second Reagent Tube, add the first detection liquid 250 μ L simultaneously, titer 8 μ L mixes and hatches 5 minutes, in the 3rd Reagent Tube, add the first detection liquid 250 μ L simultaneously, detect liquid 8 μ L and mix and hatch 5 minutes; Then in the first Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute, in the second Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute simultaneously, in the 3rd Reagent Tube, add the second detection liquid simultaneously add 50 μ L and mix and hatch 1 minute; Finally read the absorbance of the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube respectively, absorbance is compared with the titer of concentration known, thus draw the content detecting serum amyloid A protein in liquid.Described temperature when hatching is 37 DEG C.
A kind of serum amyloid A protein of the present embodiment measures kit and using method thereof, and it is by latex enhancing immune turbidimetry, and simple to operate, accuracy is high, is convenient to promote.
Embodiment 2
A kind of serum amyloid A protein measures kit, comprise the first detection liquid, second and detect liquid, first detects liquid comprises the first detection liquid Tris-HCl damping fluid, the first detection liquid sodium azide, the composition of each component is respectively by weight percentage: first detects liquid Tris-HCl damping fluid 97%, first detects liquid sodium azide 3%; Second detection liquid comprises the second detection liquid Tris-HCl damping fluid, second and detects liquid sodium azide, is coated with the latex particle of AHS's amyloid A antibody, the composition of each component is respectively by weight percentage: second detects liquid Tris-HCl damping fluid 94%, second detects liquid sodium azide 3%, is coated with the latex particle 3% of AHS's amyloid A antibody.
Serum amyloid A protein measures a using method for kit, comprises the steps: first to prepare the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube; Then in the first Reagent Tube, add the first detection liquid 250 μ L, distilled water 8 μ L mixes and hatches 5 minutes, in the second Reagent Tube, add the first detection liquid 250 μ L simultaneously, titer 8 μ L mixes and hatches 5 minutes, in the 3rd Reagent Tube, add the first detection liquid 250 μ L simultaneously, detect liquid 8 μ L and mix and hatch 5 minutes; Then in the first Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute, in the second Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute simultaneously, in the 3rd Reagent Tube, add the second detection liquid simultaneously add 50 μ L and mix and hatch 1 minute; Finally read the absorbance of the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube respectively, absorbance is compared with the titer of concentration known, thus draw the content detecting serum amyloid A protein in liquid.Described temperature when hatching is 37 DEG C.
A kind of serum amyloid A protein of the present embodiment measures kit and using method thereof, and it is by latex enhancing immune turbidimetry, and simple to operate, accuracy is high, is convenient to promote.
Embodiment 3
A kind of serum amyloid A protein measures kit, comprise the first detection liquid, second and detect liquid, first detects liquid comprises the first detection liquid Tris-HCl damping fluid, the first detection liquid sodium azide, the composition of each component is respectively by weight percentage: first detects liquid Tris-HCl damping fluid 99%, first detects liquid sodium azide 1%; Second detection liquid comprises the second detection liquid Tris-HCl damping fluid, second and detects liquid sodium azide, is coated with the latex particle of AHS's amyloid A antibody, the composition of each component is respectively by weight percentage: second detects liquid Tris-HCl damping fluid 98%, second detects liquid sodium azide 1%, is coated with the latex particle 1% of AHS's amyloid A antibody.
Serum amyloid A protein measures a using method for kit, comprises the steps: first to prepare the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube; Then in the first Reagent Tube, add the first detection liquid 250 μ L, distilled water 8 μ L mixes and hatches 5 minutes, in the second Reagent Tube, add the first detection liquid 250 μ L simultaneously, titer 8 μ L mixes and hatches 5 minutes, in the 3rd Reagent Tube, add the first detection liquid 250 μ L simultaneously, detect liquid 8 μ L and mix and hatch 5 minutes; Then in the first Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute, in the second Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute simultaneously, in the 3rd Reagent Tube, add the second detection liquid simultaneously add 50 μ L and mix and hatch 1 minute; Finally read the absorbance of the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube respectively, absorbance is compared with the titer of concentration known, thus draw the content detecting serum amyloid A protein in liquid.Described temperature when hatching is 37 DEG C.
A kind of serum amyloid A protein of the present embodiment measures kit and using method thereof, and it is by latex enhancing immune turbidimetry, and simple to operate, accuracy is high, is convenient to promote.
Claims (4)
1. a serum amyloid A protein measures kit, it is characterized in that: comprise the first detection liquid, second and detect liquid, first detects liquid comprises the first detection liquid Tris-HCl damping fluid, the first detection liquid sodium azide, the composition of each component is respectively by weight percentage: first detects liquid Tris-HCl damping fluid 97-99%, the first detects liquid sodium azide 1-3%; Second detection liquid comprises the second detection liquid Tris-HCl damping fluid, second and detects liquid sodium azide, is coated with the latex particle of AHS's amyloid A antibody, the composition of each component is respectively by weight percentage: second detects liquid Tris-HCl damping fluid 94-98%, second detects liquid sodium azide 1-3%, is coated with the latex particle 1-3% of AHS's amyloid A antibody.
2. a kind of serum amyloid A protein according to claim 1 measures kit, it is characterized in that: first detects liquid comprises the first detection liquid Tris-HCl damping fluid, the first detection liquid sodium azide, the composition of each component is respectively by weight percentage: first detects liquid Tris-HCl damping fluid 98%, first detects liquid sodium azide 2%; Second detection liquid comprises the second detection liquid Tris-HCl damping fluid, second and detects liquid sodium azide, is coated with the latex particle of AHS's amyloid A antibody, the composition of each component is respectively by weight percentage: second detects liquid Tris-HCl damping fluid 96%, second detects liquid sodium azide 2%, is coated with the latex particle 2% of AHS's amyloid A antibody.
3. a kind of serum amyloid A protein according to claim 1 measures the using method of kit, it is characterized in that comprising the steps: first to prepare the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube; Then in the first Reagent Tube, add the first detection liquid 250 μ L, distilled water 8 μ L mixes and hatches 5 minutes, in the second Reagent Tube, add the first detection liquid 250 μ L simultaneously, titer 8 μ L mixes and hatches 5 minutes, in the 3rd Reagent Tube, add the first detection liquid 250 μ L simultaneously, detect liquid 8 μ L and mix and hatch 5 minutes; Then in the first Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute, in the second Reagent Tube, add the second detection liquid add 50 μ L and mix and hatch 1 minute simultaneously, in the 3rd Reagent Tube, add the second detection liquid simultaneously add 50 μ L and mix and hatch 1 minute; Finally read the absorbance of the first Reagent Tube, the second Reagent Tube, the 3rd Reagent Tube respectively, absorbance is compared with the titer of concentration known, thus draw the content detecting serum amyloid A protein in liquid.
4. a kind of serum amyloid A protein according to claim 3 measures the using method of kit, it is characterized in that: described in temperature when hatching be 37 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510578749.9A CN105203771A (en) | 2015-09-14 | 2015-09-14 | Serum amyloid protein A determination kit and use method thereof |
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| CN201510578749.9A CN105203771A (en) | 2015-09-14 | 2015-09-14 | Serum amyloid protein A determination kit and use method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548571A (en) * | 2016-02-02 | 2016-05-04 | 潍坊三维生物工程集团有限公司 | Kit and method for detecting content of serum amyloid protein A and application |
| CN105929173A (en) * | 2016-04-27 | 2016-09-07 | 深圳市国赛生物技术有限公司 | Detection kit and detection method of serum amyloid A protein |
| CN106018829A (en) * | 2016-06-22 | 2016-10-12 | 浙江达美生物技术有限公司 | Testing reagent for serum amyloid protein A and preparation method of testing reagent |
| CN106290907A (en) * | 2016-08-03 | 2017-01-04 | 宁波普瑞柏生物技术有限公司 | Serum amyloid A protein quantitative detecting reagent and detection method in whole blood |
| CN106353507A (en) * | 2016-08-24 | 2017-01-25 | 武汉生之源生物科技股份有限公司 | Kit for detecting serum amyloid protein and application thereof |
| CN107015004A (en) * | 2017-03-21 | 2017-08-04 | 深圳市汇松科技发展有限公司 | A kind of serum amyloid A protein determines kit and preparation method thereof |
-
2015
- 2015-09-14 CN CN201510578749.9A patent/CN105203771A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548571A (en) * | 2016-02-02 | 2016-05-04 | 潍坊三维生物工程集团有限公司 | Kit and method for detecting content of serum amyloid protein A and application |
| CN105929173A (en) * | 2016-04-27 | 2016-09-07 | 深圳市国赛生物技术有限公司 | Detection kit and detection method of serum amyloid A protein |
| CN106018829A (en) * | 2016-06-22 | 2016-10-12 | 浙江达美生物技术有限公司 | Testing reagent for serum amyloid protein A and preparation method of testing reagent |
| CN106290907A (en) * | 2016-08-03 | 2017-01-04 | 宁波普瑞柏生物技术有限公司 | Serum amyloid A protein quantitative detecting reagent and detection method in whole blood |
| CN106290907B (en) * | 2016-08-03 | 2018-07-06 | 宁波普瑞柏生物技术股份有限公司 | Serum amyloid A protein quantitative detecting reagent and detection method in whole blood |
| CN106353507A (en) * | 2016-08-24 | 2017-01-25 | 武汉生之源生物科技股份有限公司 | Kit for detecting serum amyloid protein and application thereof |
| CN107015004A (en) * | 2017-03-21 | 2017-08-04 | 深圳市汇松科技发展有限公司 | A kind of serum amyloid A protein determines kit and preparation method thereof |
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Application publication date: 20151230 |