CN115541867A

CN115541867A - Biological sample joint inspection equipment and joint inspection method

Info

Publication number: CN115541867A
Application number: CN202110738306.7A
Authority: CN
Inventors: 刘治志
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2021-06-30
Filing date: 2021-06-30
Publication date: 2022-12-30

Abstract

The invention provides a biological sample joint inspection device and a joint inspection method, wherein the joint inspection device comprises an instrument host, a sampling mechanism and a plurality of detection modules are arranged in the instrument host, the plurality of detection modules at least comprise a first detection module and a second detection module, the first detection module is an immune turbidimetric detection module or an immunochromatography detection module, the sampling mechanism moves between a sampling position and a plurality of sample separation positions along a sample separation path, each sample separation position is arranged corresponding to one of the detection modules, the sampling mechanism absorbs a biological sample at the sampling position and divides the absorbed biological sample into a plurality of detection modules at the plurality of sample separation positions, and the plurality of detection modules relatively and independently carry out joint inspection on different biological markers in the biological sample.

Description

Biological sample joint inspection equipment and joint inspection method

Technical Field

The invention relates to the technical field of biological sample detection, in particular to a biological sample joint detection device and a joint detection method.

Background

The clinical detection mainly adopts an immunological method, namely, the content of various markers in a biological sample (such as blood, urine and the like) is detected by utilizing antigen-antibody reaction, and a basis is provided for disease diagnosis and the like.

The expert concurs to provide that none of the biomarkers is absolutely sensitive and absolutely specific, and can not diagnose diseases by only changing one marker, so that the requirement of joint detection of a plurality of markers exists, but the detection methods of different markers are different, so that the joint detection of the biomarkers needs to use a plurality of different detection devices, a plurality of tubes of samples need to be taken, the sample consumption is large, and the patient is not favored; in addition, for hospitals, various devices need to be purchased, so that the floor space is increased, and the maintenance cost is increased.

Disclosure of Invention

In view of the above, a biological sample joint inspection apparatus and a joint inspection method capable of simultaneously detecting a plurality of markers are provided.

In one aspect, the present invention provides a biological sample joint inspection apparatus, including an apparatus host, a sampling mechanism and a plurality of detection modules are arranged in the apparatus host, the plurality of detection modules at least include a first detection module and a second detection module, the first detection module is an immunoturbidimetric immunoassay detection module or an immunochromatographic detection module, the sampling mechanism moves between a sampling position and a plurality of sample separation positions along a sample separation path, each sample separation position is arranged corresponding to one of the detection modules, the sampling mechanism sucks a biological sample at the sampling position and separately injects the sucked biological sample to the plurality of detection modules at the plurality of sample separation positions, and the plurality of detection modules perform joint inspection on different biological markers in the biological sample relatively independently.

Further, the sample dividing path is a straight path in the X direction, a straight path in the Y direction, a straight path combining the X direction and the Y direction, or an arc path on an X-Y horizontal plane.

Further, the biological sample joint inspection equipment comprises an automatic sample feeding mechanism, wherein the automatic sample feeding mechanism is used for conveying the test tube rack loaded with the test tubes, the automatic sample feeding mechanism is provided with a sample feeding path, and the sample feeding path is parallel to the X direction.

Further, the center of the sampling bit and the centers of the plurality of decimation bits are located on an axis extending along a decimation path.

Furthermore, a dilution pool for diluting the biological sample is further arranged in the instrument host, the dilution pool and the plurality of detection modules are arranged along the sample distribution path, and the center of the dilution pool is located on the axis extending along the sample distribution path.

Further, the dilution tank is arranged between the first detection module and the second detection module.

Further, still be provided with in the instrument host computer and be used for wasing the washing pond of the sampling needle of sampling mechanism, wash the pond with a plurality of detection module are arranged along divide the appearance route, the center of wasing the pond is located along dividing the axis that the appearance route extends.

Further, a reagent storage module is further arranged in the instrument host, and the reagent storage module and the plurality of detection modules are arranged along the sample distribution path.

Furthermore, a plurality of reagent bottles are arranged in the reagent storage module and used for storing reagents required by detection, a reagent suction position is arranged on the sample dividing path corresponding to each reagent bottle, and the center of the reagent suction position is located on the axis extending along the sample dividing path.

Further, the reagent storage module is arranged between the first detection module and the second detection module.

Furthermore, the Y direction is mutually perpendicular to the X direction, a hand grip blending mechanism is further arranged in the instrument host, and the hand grip blending mechanism comprises a gripping position located on a sample feeding path of the automatic sample feeding mechanism and a blending position higher than the position of a test tube rack on the sample feeding path.

Furthermore, the sample dividing path is a straight path in the Y direction, and the sampling position is arranged on the sample feeding path and behind the grabbing position.

Further, divide the appearance route to be the ascending sharp route of X side, the sampling position is located in the instrument host computer, still be provided with transport mechanism in the instrument host computer, transport mechanism remove along a transfer route with the loading have the test tube of the biological sample that awaits measuring by mixing the position transport extremely the sampling position, transfer route with divide the appearance route to intersect perpendicularly.

Furthermore, the first detection module is an immunoturbidimetric detection module, the second detection module is a blood conventional detection module, the immunoturbidimetric detection module is provided with at least one reaction tank, the blood conventional detection module comprises at least one detection tank, and the reaction tank and the detection tank are arranged on the sample separation path; or, the first detection module is an immunochromatography detection module, the second detection module is a blood routine detection module, the immunochromatography detection module comprises at least one detection reagent card, the blood routine detection module comprises at least one detection cell, and the detection reagent card and the detection cell are arranged on the sample separation path; or, the first detection module is an immunoturbidimetric detection module, the second detection module is an immunochromatography detection module, the immunoturbidimetric detection module is provided with at least one reaction tank, the immunochromatography detection module comprises at least one detection reagent card, and the reaction tank and the detection reagent card are arranged on the sample separation path; or, first detection module is immune turbidimetric detection module, second detection module is the chemiluminescence detection module, immune turbidimetric detection module is equipped with at least one reaction tank, the chemiluminescence detection module is including testing the cup, the reaction tank with testing the cup and locating divide on the appearance route.

Further, the plurality of detection modules further include a third detection module, the third detection module is a blood routine detection module and is used for detecting blood routine parameters, the third detection module outputs hematocrit or hematocrit, and the hematocrit or hematocrit is used for correcting detection results of the first detection module and the second detection module.

In another aspect, the present invention provides a biological sample joint inspection method using the above biological sample joint inspection apparatus, the method including the steps of: sample introduction, namely conveying the test tube loaded with the biological sample to be detected to a sampling position; sampling, wherein a sampling mechanism moves along a sample dividing path to suck a biological sample from the test tube and divides the biological sample into the detection modules; and joint detection, wherein each detection module performs joint detection on different markers in the biological sample relatively independently.

Further, after the sampling mechanism dispenses the biological sample to each detection module, the sampling mechanism moves along a sample dispensing path to suck a reagent required by detection and fills the reagent to the corresponding detection module; alternatively, the sampling mechanism dispenses a biological sample to each of the detection modules, and then fills a reagent to the corresponding detection module through a reagent transfer line.

Further, the sampling mechanism moves to a cleaning position along a sample dividing path to clean the sampling needle after dispensing a biological sample to each detection module; or the sampling mechanism moves to a cleaning position along a sample dividing path to clean the sampling needle after dispensing a biological sample to each detection module and dispensing a reagent to the corresponding detection module.

Further, the sampling mechanism moves to a dilution position along the sample distribution path to dilute the biological sample before dispensing the biological sample to each of the detection modules.

Compared with the prior art, the biological sample joint inspection equipment and the joint inspection method have the advantages that the detection modules are sequentially arranged, the biological sample to be detected and the corresponding reagent are separately injected into the detection modules through the sampling mechanism to synchronously carry out joint inspection on various markers, one instrument can sample at one time and divide the sample for multiple times, the detection of multiple projects is completed simultaneously, the blood collection amount is reduced, and the blood collection pain of a patient is relieved; in addition, a plurality of detection modules of one instrument detect simultaneously, shorten the detection turnaround time of a plurality of items, promote detection efficiency, reduce the time of output sample inspection report to patient's latency has been shortened. The invention integrates various methodologies into one device, simplifies the operation flow and reduces the purchase and maintenance cost of the instrument.

Drawings

Fig. 1 is a block diagram of a biological sample joint inspection apparatus according to a first embodiment of the present invention.

Fig. 2 is a schematic view of the internal layout of the biological sample joint inspection apparatus shown in fig. 1.

Fig. 3 is a schematic diagram of the internal layout of a biological sample joint inspection apparatus according to a second embodiment of the present invention.

Detailed Description

To facilitate an understanding of the invention, the invention will now be described more fully hereinafter with reference to the accompanying drawings. One or more embodiments of the present invention are illustrated in the accompanying drawings to provide a more accurate and thorough understanding of the disclosed embodiments. It should be understood, however, that the present invention may be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein.

The invention provides a biological sample joint inspection device and a joint inspection method, which can realize synchronous detection of a plurality of markers in a biological sample by one biological sample. The biological sample joint inspection equipment comprises an instrument host 10 and an external controller 30, wherein the external controller 30 can be a PC or the like and is used for interacting with an operator or the outside; the apparatus main unit 10 is used for performing specific detection operations, and a plurality of detection modules are arranged in the apparatus main unit 10 in parallel, so that various markers of the biological sample can be synchronously detected respectively. It should be noted that the parallel arrangement in the present invention means that the detection processes of the detection modules are performed synchronously rather than sequentially. When the invention is used, an operator sets a corresponding joint inspection program through the external controller 30 according to the type of a biological sample to be detected, and the instrument host 10 starts a corresponding detection module to carry out specific detection according to the joint inspection program set by the external controller 30.

As shown in fig. 1-2, in the first embodiment of the present invention, the apparatus host 10 includes a sample injection mechanism 12, a transport mechanism 14, a sampling mechanism 16, and a plurality of

detection modules

18 and 19.

The sample injection mechanism 12 is disposed outside the housing of the main instrument 10, and is used for automatically transporting the test tube 50 containing the biological sample to be tested, such as blood sample, to the sample injection port of the main instrument 10. Typically, multiple tubes 50 are placed on the same tube rack 52, with each tube 50 carrying a biological specimen from a patient. The sample injection mechanism 12 drives the entire test tube rack 52 to move toward the sample inlet along a sample injection path, which extends along a first direction (i.e., the X direction in the figure). The transfer mechanism 14 is disposed in the housing of the main instrument unit 10, and moves along a transfer path 15 to transfer the test tube 50 to a position directly below the sampling site a, and the transfer path 15 extends in the second direction (i.e., the Y direction in the figure). The sampling mechanism 16 moves within the housing along a sampling path 17 between a sampling position a at which a sampling needle of the sampling mechanism 16 aspirates a biological sample within the test tube 50 and a plurality of sampling positions B, C; at each of the dispensing positions B, C, the sampling needle dispenses a predetermined dose of the biological sample into each of the

corresponding detection modules

18, 19.

In the illustrated embodiment, there are two detection modules, hereinafter referred to as the first detection module 18 and the second detection module 19, respectively. The first detection module 18 is used for detecting a marker with a high concentration level, such as milligram level, in a biological sample; the second detection module 19 is used for detecting markers with low concentration levels in the biological sample, such as nanogram level or even picogram level. In an embodiment of the present invention, for example, when the detection module is used for detecting a blood sample, the first detection module 18 may be an immunoturbidimetric detection module, such as a transmittance turbidimetric detection module, a scattering turbidimetric detection module, and the like, and is used for detecting concentrations of C-reactive protein (CRP) and Serum Amyloid A (SAA) in the blood sample; the second detection module 19 may be an immunochromatography detection module, such as a fluorescence immunochromatography detection module, a colloidal gold immunochromatography detection module, a quantum dot immunochromatography detection module, a time-resolved immunochromatography detection module, and the like, and is used for detecting the concentration of Procalcitonin (PCT) and interleukin 6 (IL-6) in a blood sample.

CRP is a non-specific marker in inflammation or tissue injury, and the rising amplitude of CRP is positively correlated with the degree of infection; SAA is an acute phase protein produced by the liver, and the concentration of SAA is obviously increased when a human body has acute inflammatory injury. PCT is the propeptide of calcitonin, whose production is closely related to the release of endotoxins and inflammatory mediators of bacterial infection; IL-is a pleiotropic cytokine with a wide range of functions, and is rapidly produced during acute inflammatory reactions. The disease condition of the patient can be accurately judged by joint detection of four markers of CRP, SAA, PCT and IL-6 in a blood sample. In some embodiments, the second detection module 19 can also be a chemiluminescence detection module, which can be used not only for detection of PCT, IL-6 in a blood sample, but also for detection of markers such as T3, T4, AFP, and the like.

The biological sample joint inspection equipment can be used for detecting the concentration of inflammation markers such as CRP, SAA, PCT and IL-6 and also can be used for quantitative analysis of cardiac markers, respiratory markers and gastric function markers. The cardiac muscle markers include D-Dimer (D-Dimer, D-D), serum cardiac protein T (TnT), B-type natriuretic peptide (BNP, which may also be referred to as brain natriuretic peptide), cardiac troponin (cTn), amino terminal brain natriuretic peptide precursor (NT-proBNP), and the like; respiratory markers include syncytial virus and adenovirus; markers of gastric function include, but are not limited to, pepsinogen and gastrin. The specific embodiments of the present invention are described with reference to inflammation markers.

In the illustrated embodiment, the first detection module 18 and the second detection module 19 are sequentially arranged along the X direction, and each of the

detection modules

18 and 19 includes at least one detection position, where the detection position may be an immunoturbidimetric reaction chamber, an immunochromatographic detection reagent card, a chemiluminescent test cup, a conventional blood impedance cell, or the like. The detection position of the first detection module 18 is located right below the sample splitting position B, and the detection position of the second detection module 19 is located right below the sample splitting position C. Preferably, the center of the sampling position a and the centers of the sample splitting positions B and C are located on a transverse axis extending along the X direction, and the sampling mechanism 16 moves back and forth along the sample splitting path 17 along the X direction to sample the detection positions of the test tube 50 and the first and

second detection modules

18 and 19 directly below, so that the sampling/sample splitting movement is a linear movement, which can simplify the arrangement of the sample splitting path 17 of the sampling mechanism 16. Preferably, the sample separating path 17 is disposed above the transferring path 15 and vertically intersects with the transferring path, and the intersecting position is the sampling position a, so that the transferring mechanism 14 can transport the test tube 50 to the position right below the sampling needle, so that the sampling needle can accurately puncture for sampling, and the damage of the sampling needle due to incorrect alignment is avoided.

Preferably, a hand grip blending mechanism 13 is further disposed in the instrument host 10 for blending the biological sample. For example, the blood sample is mainly composed of blood cells and plasma, and due to the difference of specific gravity between the blood cells and the plasma, the blood sample may be layered after a period of standing, and if the blood sample is not sufficiently mixed, the measurement result may be greatly deviated. The hand grip blending mechanism 13 comprises a gripping position on the sample feeding path and a blending position D higher than the position of the test tube rack 52, and the transfer mechanism 14 firstly moves the test tube 50 to the blending position D before conveying the test tube 50 to the sampling position a. At the mixing position D, the gripping and mixing mechanism 13 grips the test tube 50 and uniformly mixes the biological sample in the test tube 50 by rotating, reversing, vibrating, and the like. Preferably, the mixing bit D and the sampling bit a are arranged along the Y direction, and the centers of the mixing bit D and the sampling bit a are located on a longitudinal axis extending along the Y direction. It should be noted that, there may be a certain height difference between the blending position D and the sampling position a in the vertical direction, and the fact that the centers of the blending position D and the sampling position a are located on a longitudinal axis means that the projections of the centers of the blending position D and the sampling position a on a horizontal plane are located on a longitudinal axis. In other embodiments, the decimation path 17 may be a linear path in the Y-direction, a linear path in which the X-direction and the Y-direction are combined, or an arc path in the X-Y horizontal plane. When the sample separation path is a straight path in the Y direction 17, the sampling position a may be disposed on the sample introduction path and behind the grasping position of the blending mechanism 13, and the transfer mechanism 14 may be omitted.

Preferably, the sample injection mechanism 12 is provided with a test tube presence detection station, a test tube barcode recognition station, a test tube type recognition station, and the like. After each detection, the test tube 50 is transported to the blending mechanism 13 by the transportation mechanism 14 for blending. At the test tube presence or absence detection station, the system automatically identifies whether a test tube 50 is input, and if not, all subsequent processes are stopped. At the test tube barcode identification station, the system automatically identifies the barcode on the test tube 50, which typically contains information about the patient, including name, age, contact details, etc., by which the patient can be directly associated with the test results. At the test tube type identification station, the system automatically identifies whether the type of the test tube 50 is a normal tube, which is commonly used to carry whole blood and has a large amount of blood, or a micro tube, which is commonly used to carry tip blood and has a small amount of blood. The mixing mechanism 13 performs different mixing operations according to different types of test tubes 50, such as mixing by inversion or rotation for the normal tube mixing mechanism 13, and mixing by vibration for the micro tube mixing mechanism 13.

When the invention is used for detecting biological samples, the invention mainly comprises the following steps: feeding a sample, namely conveying a test tube 50 carrying a biological sample to be tested to the instrument host 10; mixing evenly, namely mixing the biological samples to be detected in the test tube 50 evenly; transferring, namely conveying the uniformly mixed test tube 50 to a sampling position A; sampling and dispensing, wherein the biological sample in the test tube 50 is sucked and moved along the sample dispensing path 17 to dispense the sucked biological sample to each

detection module

18, 19; and (4) performing joint detection, reacting the biological sample with corresponding reagents in each

detection module

18 and 19, and outputting a detection result. Because the

detection modules

18 and 19 run synchronously, the detection results of various markers in the biological sample can be obtained as fast as possible, and the illness state of the patient can be comprehensively judged according to various results. The joint inspection apparatus and joint inspection method according to the first embodiment of the present invention will be described below by taking the application to the detection of an inflammation marker in a blood sample as an example.

The specific detection process of the joint inspection equipment applied in the embodiment is as follows:

first, the test tube 50 carrying a biological sample to be tested, the immunoturbidimetric reagent, the immunochromatographic card strip, and the like are put into the apparatus main unit 10, and the joint inspection program is set on the external controller 30 to start the apparatus main unit 10. The test tube 50 may be automatically transported toward the apparatus main unit 10 by the sample injection mechanism 12, or may be manually inserted by an operator, and the automatic sample injection is usually performed when performing batch detection of blood samples, and the manual sample injection may be performed when detecting individual blood samples. After the instrument host 10 is started, firstly identifying the type and relevant information of the test tube 50, then executing corresponding blending operation according to the type blending mechanism 13 of the test tube 50, and conveying the blended test tube 50 to a sampling position A by the transfer mechanism 14;

at the sampling position a, the sampling needle of the sampling mechanism 16 sucks a certain volume of blood sample from the test tube 50, and then the sampling mechanism 16 sequentially moves to the sample separating position B corresponding to the first detection module 18 and the sample separating position C corresponding to the second detection module 19 and dispenses the sucked blood sample to the reaction cups of the first detection module 18 and the second detection module 19. The sampling needle is cleaned after dispensing of the blood sample is completed, and is ready for the next detection. While the sampling mechanism 16 dispenses the blood sample, the immune turbidimetric reagent with a corresponding dosage, including the R1 reagent, the R2 (such as latex) reagent, and the like, is respectively extracted from the reagent bottle by a quantitative device such as a syringe, a quantitative pump, and the like, and is injected into the reaction cup of the first detection module 18; the corresponding amount of sample diluent is injected into the reaction cup of the second detection module 19 by a quantitative device such as a syringe and a quantitative pump to dilute the blood sample. Specifically, the method comprises the following steps:

in the first detection module 18, i.e., the immunoturbidimetric detection module, the reagent R1 is first added to the blood sample to lyse red blood cells, and then the antibody-bearing R2 (latex) reagent is added to the blood sample to cause an antigen-antibody reaction with a marker such as CRP or SAA. And then carrying out immunoturbidimetry detection, turning on a light source to irradiate the reaction cup, collecting scattered light signals after light rays pass through the reaction cup, automatically recording reaction time and signal intensity by the system, calculating the change speed or light intensity change value of the scattered light intensity, and automatically calculating the concentration of specific proteins such as CRP (C-reactive protein) or SAA (serum amyloid antigen) in the blood sample through a calibration curve.

In the second detection module 19, i.e. the immunochromatographic detection module, the diluted blood sample is added to the immunochromatographic detection card strip, the immunochromatographic detection card strip is transferred to the incubation module and incubated for a period of time, and the markers such as PCT and IL-6 and the like are immunoreactive with the fluorescent-labeled antibody. And then carrying out immunochromatography detection, opening an excitation light source to irradiate a detection area and a quality control area of the detection card strip, exciting the solidified fluorescent immune complex to emit fluorescence, strictly correlating the intensity of a fluorescence signal with the content of a marker in the blood sample to be detected, and automatically calculating the concentration of PCT, IL-6 and the like in the blood sample through a standard curve.

The detection processes of the

detection modules

18 and 19 are performed synchronously, and the detection results can be automatically uploaded to the external controller 30 and displayed to the operator. After the detection is finished, the system starts a cleaning program, and a cleaning agent is conveyed to each

detection module

18 and 19 to clean the core components of each

detection module

18 and 19 and the connecting pipeline between the

detection modules

18 and 19, so as to prepare for the next detection.

Fig. 3 is a schematic diagram of a joint inspection apparatus according to a second embodiment of the present invention, which is different from the first embodiment mainly in that: in this embodiment, a reagent storage module 20 is disposed in the instrument host 10, and a plurality of reagent bottles are disposed in the reagent storage module 20, and are used for storing various reagents required for detection, such as an immunoturbidimetric reagent, an immunochromatographic reagent, and the like. The reagent storage module 20 and the

detection modules

18 and 19 are arranged along the X direction, the sample dividing path 17 of the sampling mechanism 16 is provided with a reagent suction position E corresponding to each reagent bottle of the reagent storage module 20, and the reagent suction position E is over against the center of the reagent bottle. Preferably, the sampling site a, the sample splitting sites B and C, and the reagent aspirating site E are all centered on a horizontal axis extending along the X direction.

In this embodiment, the sampling needle of the sampling mechanism 16 may sequentially move to each reagent aspirating position E to aspirate a required reagent after the sampling at the sampling position a and the blood sample dispensing at each of the sample dispensing positions B and C are completed, and after each reagent aspirating position E aspirates a certain amount of reagent, the sampling mechanism 16 moves to the sample dispensing position B or C corresponding to the

corresponding detection module

18 or 19 and injects the aspirated reagent into the corresponding

detection module

18 or 19. Thus, in this embodiment, the sampling needle of the sampling mechanism 16 can complete the sampling and dispensing operations of the blood sample and the corresponding reagent, and there is no need to additionally provide a syringe for each reagent, so that the devices in the main instrument 10 can be simplified, and the overall layout of the joint inspection apparatus of the present invention is more flexible. In other embodiments, reagents may be added to the corresponding

detection modules

18, 19 via reagent delivery lines.

Preferably, as shown in fig. 2, a dilution well is further disposed in the apparatus main body 10 for performing a dilution process of the blood sample. The detection method is limited, the blood sample with too high concentration cannot be accurately detected, and can be accurately detected only by diluting dozens of times or even hundreds of times to be within the linear range of the standard curve. The blood sample and the diluent are fully mixed in the diluting pool and then are conveyed to each

reaction module

18 and 19, in the embodiment, the diluting pool and each

detection module

18 and 19 are distributed along the X direction, the sample distributing path 17 of the sampling mechanism 16 is provided with a diluting position F corresponding to the diluting pool, preferably, the center of the diluting pool or the diluting position F and the centers of the sampling position A, the sample distributing position B and the sample distributing position C are both positioned on a horizontal axis extending along the X direction, and the sampling needle of the sampling mechanism 16 is positioned right above the cleaning pool when the sampling mechanism 16 moves to the diluting position F along the X direction.

Preferably, as shown in fig. 2, a cleaning tank filled with a certain dosage of cleaning agent is also arranged in the instrument main body 10 for cleaning the sampling needle of the sampling mechanism 16. The cleaning pool and each

detection module

18, 19 are distributed along the X direction, the sample dividing path 17 of the sampling mechanism 16 is provided with a cleaning position G corresponding to the cleaning pool, preferably, the center of the cleaning pool or the cleaning position G and the centers of the sampling position A, the sample dividing position B, and the sampling position C are all positioned on a horizontal axis extending along the X direction, and the sampling needle of the sampling mechanism 16 moves to the position right above the cleaning pool when moving to the cleaning position G along the X direction. It should be noted that the diluting pool, the cleaning pool, the sampling mechanism 16 and the detecting

modules

18 and 19 may have a certain height difference, and the fact that the centers are located on a horizontal axis means that the projections of the centers on a horizontal plane are on the same horizontal axis.

The joint inspection equipment and the joint inspection method of the present invention have been roughly described by taking the detection of the inflammation marker in the blood sample as an example, it should be understood that the present invention can select different detection modules according to different biological samples to be detected, the type and number of the detection modules can be determined according to specific detection requirements, and the present invention is not limited to the above specific embodiments. As an embodiment, a third detection module, such as a blood routine detection module, may be further disposed in the instrument host, for detecting blood routine parameters such as Red Blood Cells (RBC), hemoglobin (HCG), white Blood Cells (WBC), and Platelets (PLT). The Hematocrit (HCT) or the complete hematocrit (BCV), etc. may be calculated from the blood routine parameters to correct the concentrations of CRP, SAA, PCT, IL-6 measured by the first and

second detection modules

18, 19. Similarly, the third detection module is arranged with the first and

second detection modules

18 and 19 along the X direction, and the sampling mechanism moves along the sample splitting track 17 along the X direction to realize sampling and dispensing of the sample/reagent of each detection module.

In the above embodiment, the detection modules are linearly arranged along the first direction, the sampling mechanism moves along the sample dividing path in the first direction to sample and dispense the sample/reagent, and the detection modules synchronously operate to perform joint detection on multiple markers. It should be understood that the arrangement of the detection modules is not limited to a straight arrangement, and in other embodiments, the detection modules may be arranged along an arc; correspondingly, the sample dividing path of the sampling mechanism can also be in an arc shape. In general, the biological sample joint inspection equipment can realize the detection of a plurality of items by only one biological sample, and simultaneously performs synchronous detection on the plurality of items after multiple sample distribution to a plurality of detection modules, thereby greatly shortening the time of the turnover of the samples of the existing products among a plurality of instruments; the device can simultaneously test a plurality of items, the total test time is shortened, and the patient can acquire the test report as fast as possible, which is very beneficial to the patient, especially the emergency patient. In addition, one device integrates multiple detection functions, so that the operation of medical staff is simplified, the occupied area of the device is reduced, the later maintenance of the device is facilitated, and the purchase and maintenance cost is reduced.

It should be noted that the present invention is not limited to the above-mentioned embodiments, and other changes and modifications can be made by those skilled in the art according to the spirit of the present invention, and these changes and modifications made according to the spirit of the present invention should be included in the scope of the present invention as claimed.

Claims

1. The utility model provides a biological sample joint inspection equipment, includes the instrument host computer, its characterized in that, be provided with sampling mechanism and a plurality of detection module in the instrument host computer, a plurality of detection module include first detection module and second detection module at least, first detection module is immunoturbidimetry detection module or immunochromatography detection module, sampling mechanism moves between sampling position and a plurality of branch appearance position along a branch appearance route, each divide the appearance position to correspond one of them detection module sets up, sampling mechanism is in the biological sample is drawn and is in a plurality of to divide the appearance position to divide the biological branch of absorption to divide the appearance sample to a plurality of detection module is a plurality of detection module relatively independently carries out joint inspection to the different biological markers in the biological sample.

2. The apparatus for joint inspection of biological samples according to claim 1, wherein the sample separation path is a straight path in an X-direction, a straight path in a Y-direction, a straight path in which the X-direction and the Y-direction are combined, or an arc path on an X-Y horizontal plane.

3. The apparatus for joint inspection of biological samples according to claim 2, wherein the apparatus for joint inspection of biological samples comprises an autosampler mechanism for transporting the test tube rack loaded with test tubes, the autosampler mechanism having a sample path, the sample path being parallel to the X direction.

4. The apparatus for joint inspection of biological samples according to claim 3, wherein the centers of the sampling sites and the centers of the plurality of the fractionating sites are located on an axis extending along the fractionating path.

5. The apparatus for joint inspection of biological samples according to claim 4, wherein a dilution well for diluting the biological samples is further provided in the main instrument body, the dilution well and the plurality of detection modules are arranged along the sample distribution path, and the center of the dilution well is located on the axis extending along the sample distribution path.

6. The device for joint inspection of biological samples according to claim 5, wherein the dilution well is disposed between the first detection module and the second detection module.

7. The apparatus for combined biological sample inspection according to claim 4, wherein a cleaning tank for cleaning the sampling needle of the sampling mechanism is further provided in the apparatus main body, the cleaning tank and the plurality of detection modules are arranged along the sample distribution path, and the center of the cleaning tank is located on the axis extending along the sample distribution path.

8. The apparatus for joint inspection of biological samples according to claim 4, wherein a reagent storage module is further disposed in the apparatus main body, and the reagent storage module and the plurality of detection modules are arranged along the sample distribution path.

9. The apparatus for joint inspection of biological samples according to claim 8, wherein a plurality of reagent bottles are disposed in the reagent storage module for storing reagents required for the inspection, the sample separation path is provided with a reagent suction position corresponding to each reagent bottle, and the center of the reagent suction position is located on the axis extending along the sample separation path.

10. The device for joint inspection of biological samples according to claim 8, wherein the reagent storage module is disposed between the first detection module and the second detection module.

11. The joint inspection apparatus for biological samples according to claim 4, wherein the Y direction is perpendicular to the X direction, the apparatus main body is further provided with a hand grip mixing mechanism, and the hand grip mixing mechanism comprises a gripping position located on a sample feeding path of the automatic sample feeding mechanism and a mixing position higher than a position of the test tube rack on the sample feeding path.

12. The apparatus for joint inspection of biological samples according to claim 11, wherein the sample dividing path is a straight path in the Y direction, and the sampling site is disposed on the sample introduction path and behind the grasping site.

13. The biological sample joint inspection device according to claim 11, wherein the sample dividing path is a straight path in the X direction, the sampling position is disposed in the instrument host, a transfer mechanism is further disposed in the instrument host, the transfer mechanism moves along a transfer path to transfer the test tube loaded with the biological sample to be tested from the mixing position to the sampling position, and the transfer path is perpendicular to the sample dividing path.

14. The apparatus for combined detection of biological samples according to any one of claims 1 to 13, wherein the first detection module is an immunoturbidimetric detection module, the second detection module is a conventional blood detection module, the immunoturbidimetric detection module is provided with at least one reaction cell, the conventional blood detection module comprises at least one detection cell, and the reaction cell and the detection cell are provided on the sample separation path; or, the first detection module is an immunochromatography detection module, the second detection module is a blood routine detection module, the immunochromatography detection module comprises at least one detection reagent card, the blood routine detection module comprises at least one detection cell, and the detection reagent card and the detection cell are arranged on the sample separation path; or the first detection module is an immunoturbidimetric detection module, the second detection module is an immunochromatography detection module, the immunoturbidimetric detection module is provided with at least one reaction cell, the immunochromatography detection module comprises at least one detection reagent card, and the reaction cell and the detection reagent card are arranged on the sample separation path; or, first detection module is the immunoturbidimetric detection module, the second detection module is the chemiluminescence detection module, the immunoturbidimetric detection module is equipped with at least one reaction tank, the chemiluminescence detection module includes the test cup, the reaction tank with the test cup is located divide on the appearance route.

15. The device for joint examination of biological samples according to any one of claims 1-13, wherein the plurality of test modules further comprises a third test module, wherein the third test module is a blood routine test module for testing blood routine parameters, and the third test module outputs hematocrit or pancyto-hematocrit for correcting the test results of the first test module and the second test module.

16. A biological sample joint inspection method using the biological sample joint inspection apparatus according to any one of claims 1 to 15, the method comprising the steps of:

sample introduction, namely conveying the test tube loaded with the biological sample to be detected to a sampling position;

sampling, wherein a sampling mechanism moves along a sample dividing path to suck a biological sample from the test tube and divides the biological sample into the detection modules;

and joint detection, wherein each detection module performs joint detection on different markers in the biological sample relatively independently.

17. The joint inspection method for biological samples according to claim 16, wherein the sampling mechanism moves the reagent required for the aspiration detection along the sample separation path and fills the reagent into the corresponding detection module after dispensing the biological sample into each detection module; alternatively, the sampling mechanism dispenses a biological sample to each of the detection modules, and then fills a reagent to the corresponding detection module through a reagent transfer line.

18. The method for joint inspection of biological samples according to claim 17, wherein the sampling mechanism moves to a cleaning position along a sample dividing path to clean the sampling needle after dispensing the biological sample to each of the detection modules; or after dispensing a biological sample to each detection module and dispensing a reagent to the corresponding detection module, the sampling mechanism moves to a cleaning position along a sample dispensing path to clean the sampling needle.

19. The method for combined testing of biological samples according to claim 16, wherein the sampling mechanism moves to a dilution position along the sample distribution path to dilute the biological sample before the biological sample is dispensed to each of the test modules.