CN215627992U - Microbial limit intelligent detection equipment - Google Patents

Abstract

The utility model relates to the field related to microbial limit detection, in particular to an intelligent microbial limit detection device, which comprises an ISO 5-level laminar flow device, wherein a liquid suction device is arranged below the ISO 5-level laminar flow device, a conveying belt is arranged below the liquid suction device, a No. 1 robot and a No. 2 robot are arranged on two sides of the conveying belt, and a dry-type heating constant-temperature device and an automatic pipette inlet and outlet device are arranged on one side of the No. 2 robot; no. 1 robot one side is equipped with culture dish storage device, water-bath and waste material box in proper order. The sample preparation process is completed by automatic operation of equipment, and the defects of low manual operation efficiency, easy pollution to samples, large operation difference of different operators and the like are overcome.

Description

Microbial limit intelligent detection equipment

Technical Field

The utility model provides intelligent detection equipment for microbial limit, and relates to the field related to microbial limit detection.

Background

The raw and auxiliary materials used in the production process of the medicine all need to be detected to the limit of microorganisms. When the microbial limit is detected, the preparation process of the sample is carried out in a unidirectional flow air region of local cleanliness ISO5 level under the background of environmental cleanliness ISO 7 level. The whole process of the inspection must strictly comply with aseptic operation to prevent recontamination. Cleanliness of the unidirectional flow air region, the working table surface and the environment should be regularly verified according to the current national standard of testing methods for suspended particles, planktonic bacteria and sedimentary bacteria in a clean room (region) in the pharmaceutical industry. Personnel enter the clean room and need to go through strict dressing procedures, and the operation process needs extra caution.

The general steps for sample preparation include: transferring the sterilized container and the culture medium to an ISO 5-grade unidirectional flow air area under a clean environment, adding a certain amount of test sample into the culture medium solution at about 45 ℃, uniformly stirring, adding a certain amount of culture medium solution into a culture dish, and covering a culture dish cover to finish the preparation process of the sample.

The microorganism limit detection in the prior art has the defects of high requirements on the operation environment and the operation of personnel, low working efficiency, easy pollution to samples, large operation difference of different operators and the like,

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide the intelligent detection equipment for the microbial limit, which can overcome the defects, the sample preparation process is automatically operated by the equipment, and the defects of low manual operation efficiency, easy pollution to samples, large operation difference of different operators and the like are overcome.

In order to achieve the purpose, the utility model provides intelligent detection equipment for microbial limit, which comprises an ISO 5-level laminar flow device, wherein a liquid suction device is arranged below the ISO 5-level laminar flow device, a conveyor belt is arranged below the liquid suction device, a No. 1 robot and a No. 2 robot are arranged on two sides of the conveyor belt, and a dry-type heating constant-temperature device and an automatic pipette inlet and outlet device are arranged on one side of the No. 2 robot; no. 1 robot one side is equipped with culture dish storage device, water-bath and waste material box in proper order.

Preferably, the ISO 5-level laminar flow device comprises an air supply fan, a primary filter, a medium-efficiency filter and a high-efficiency filter which are sequentially arranged, and a differential pressure gauge and an anemometer are arranged in the ISO 5-level laminar flow device. The vertical unidirectional flow of air supply is realized, and a clean environment is provided for sample preparation.

Preferably, the dry-type heating thermostat comprises a heating plate and a temperature control unit connected with the heating plate, and provides a constant temperature environment for the culture medium.

Preferably, the water bath kettle comprises a container, a heating coil is arranged at the bottom of the container and connected with a water bath circulation pipeline outside the container, and the water bath circulation pipeline is connected with the temperature control unit through a water pump.

Preferably, a No. 1 robot distribution box and a No. 2 robot distribution box which are connected with the No. 1 robot and the No. 2 robot are respectively arranged below the conveyor belt.

Preferably, an automatic lid opening mechanism is also provided. The automatic cover opening mechanism realizes the cover opening operation of the culture medium bottle, the sample bottle and the container package.

Compared with the prior art, the utility model has the beneficial effects that:

the utility model better solves the technical defects in the prior art, the background area for placing the equipment can be reduced from an ISO 7-grade clean area to an ISO 9-grade area, the sample preparation process is completed by automatic operation of the equipment, and the defects of low manual operation efficiency, easy pollution to the sample, large operation difference of different operators and the like are overcome.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a wicking apparatus of the present invention;

wherein, 1, the liquid suction device is 1; 2. position 2 of the liquid suction device; 3. position 3 of the liquid suction device; 4. 4, a liquid suction device; 5. a conveyor belt; 6. a culture dish storage device; 7. robot No. 1; 8. a water bath kettle; 9. a waste material box; 10. no. 1 robot distribution box; 11. no. 2 robot distribution box; 12. a dry-type heating thermostat; 13. the automatic pipette in and out device; 14. a robot number 2; 15. an ISO class 5 layering device; 16. an automatic uncovering mechanism.

Detailed Description

Example 1

The utility model is further illustrated in the following figures 1-2, in conjunction with the accompanying drawings: the intelligent detection equipment for the microbial limit comprises an ISO 5-level laminar flow device 15, wherein a liquid suction device is arranged below the ISO 5-level laminar flow device 15, a conveying belt 5 is arranged below the liquid suction device, a No. 1 robot 7 and a No. 2 robot 14 are arranged on two sides of the conveying belt 5, and a dry-type heating constant temperature device 12 and a liquid suction pipe automatic in-and-out device 13 are arranged on one side of the No. 2 robot 14; no. 1 robot 7 one side is equipped with culture dish storage device 6, water-bath 8 and waste material box 9 in proper order.

The ISO 5-level laminar flow device 15 comprises an air supply fan, a primary filter, a medium-efficiency filter and a high-efficiency filter which are sequentially arranged, and a differential pressure gauge and an anemometer are arranged in the ISO 5-level laminar flow device 15.

The dry-type heating thermostat 12 includes a heating plate and a temperature control unit connected to the heating plate.

Still including being located the water bath constant temperature equipment of No. 1 robot 7 or No. 2 robot 14 one side, water bath constant temperature equipment includes the container, and the container bottom is equipped with heating coil is connected with the outer water bath circulation pipeline of container, and water bath circulation pipeline passes through the water pump and is connected with the temperature control unit. No. 1 robot block terminal 10, No. 2 robot block terminal 11 that the conveyer belt 5 below was equipped with respectively and is connected with No. 1 robot 7 and No. 2 robot 14.

An automatic lid opening mechanism 16 is also included.

The pipetting device is shown in figure 2. The structure and the working principle are as follows: the servo motor runs forward and backward to drive the syringe handle fixed on the sliding table slide block to move forward and backward so as to simulate the manual push-pull of the syringe to finish the row and liquid suction, and the needle heads of the 4 groups of syringes are respectively connected with the No. 1, No. 2, No. 3 and No. 4 positions of the liquid suction device through hoses to provide power for sucking and discharging the liquid. The liquid suction device provides power for sucking and discharging liquid.

The pipette is a storage tube for storing and sucking and discharging liquid, the pipette automatic in-and-out device stores a cleaned and clean sterilized tube, the used (stored liquid) pipette which is conveyed out by the conveying belt is reused after being cleaned and sterilized again. The water bath can display the temperature and set the temperature, which can accelerate the dissolution of the medicine, and the operation flow is described as follows: the drug is weighed manually, the drug is placed in the conical flask and placed in a water bath pot manually, the conical flask is clamped by a robot to be placed below the pipette, and the diluent is added into the conical flask (because the temperature of the diluent is room temperature, the drug is difficult to dissolve in the diluent, and the diluent is heated to be dissolved quickly).

Taking a detection process of the limit of microorganisms as an example, the functions of the intelligent device are further explained.

1. And (4) opening the equipment, opening the ISO 5-level laminar flow device 15, and opening the dry-type heating thermostat 12 and the water bath 8.

2. The pipette, pipette box, petri dish, bottle cap box, sample bottle, sample, sterile peptone water solution bottle and culture medium bottle are transferred under the ISO5 grade laminar flow device 15 by the conveyor belt 5, and placed to the designated position by the No. 1 robot 7 and the No. 2 robot 14. Wherein the bottle cap box is used for placing a sterile peptone water solution bottle cap and a culture medium bottle cap.

3. When transferring aseptic peptone water solution bottle, culture medium bottle, touch contact switch when the bottle, the action is opened: the No. 2 robot 14 clamps the bottle neck, the No. 1 robot 7 uncaps the bottle and puts the bottle cap into the bottle cap box, and the No. 2 robot 14 puts the sterile peptone water solution bottle and the culture medium bottle into a designated dry-type heating thermostat in sequence for heat preservation and heating.

4.14 get the pipette of No. 2 robot and put into No. 1 position of imbibition device, No. 2 robot clamp aseptic peptone water solution bottle to No. 1 pipette suction opening department, and the imbibition device absorbs sterile peptone water solution of specified quantity, and No. 1 robot 7 presss from both sides the sample bottle to No. 1 pipette suction opening department aseptic peptone water solution of specified quantity is put into to the sample bottle, then No. 1 robot 7 presss from both sides the sample bottle and shakes even operation.

5. If the sample needs to be bathed, the No. 1 robot 7 clamps the sample bottle and puts the sample bottle into a water bath pan to shake up until the set time is reached.

No. 6.2 robot 14 gets the pipette respectively and puts into No. 2 positions, No. 3 positions, No. 4 positions of suction device, and No. 2 robots are got and are got culture medium bottle to No. 2 pipette suction opening department, and suction device absorbs the culture medium of specified quantity.

No. 7.1 robot 7 presss from both sides and gets sample bottle to No. 4 pipette suction opening department, the sample of the specified quantity that the imbibition device absorb, and No. 1 robot 7 places the sample bottle conveyer belt 5 with the sample and shifts out remaining sample.

8. 6 automatic play two dishes of culture dish dishes of having killed fungus of culture dish storage device, then open culture dish lid by No. 1 robot, No. 2 robot gets culture dish to No. 4 pipette suction ports departments and No. 2 pipette suction ports departments, puts into to the culture dish with the culture medium of the specified quantity that imbibition device absorbs the sample of specified quantity and imbibition device absorption, and No. 2 robot shakes and puts into conveyer belt 5 after even.

No. 9.1 robot 7 continues to open second dish culture dish lid after with culture dish lid upper cover, and No. 2 robot 14 gets culture dish to No. 4 pipette suction ports department and No. 2 pipette suction ports department, and the specified quantity's that the imbibition device absorbed the sample of specified quantity and imbibition device absorbs culture medium is put into to the culture dish, and 2 # robot 14 shakes and puts into conveyer belt 5 after even.

10.14 clamps of No. 2 robot 14 and gets culture medium bottle to No. 3 pipette suction ports department, the liquid suction device absorbs the culture medium of specified quantity, culture dish storage device 6 goes out two dishes automatically and has disinfected the culture dish, then open culture dish lid by No. 1 robot 7, No. 2 robot 14 gets culture dish to No. 4 pipette suction ports department and No. 2 pipette suction ports department, put into the culture medium of specified quantity of the sample that absorbs specified quantity and liquid suction device absorption with liquid suction device in the culture dish, 2 No. 14 robot shakes and puts into conveyer belt 5 after even.

No. 11.1 robot 7 continues to open second dish culture dish lid after with culture dish lid upper cover, 14 take culture dish dishes to No. 4 pipette suction orifices departments and No. 3 pipette suction orifices departments of No. 2 robots, the culture medium of the specified quantity that imbibition device absorbs the sample of specified quantity and imbibition device absorption is put into to the culture dish, 14 shake after even put into conveyer belt 5 of No. 2 robots, accomplish a batch of sample detection work, four dish culture dishes are shifted out equipment by conveyer belt 5, continue next batch of work, reciprocating cycle.

12. After the last batch is finished, the robot No. 2 14 puts the used pipettes into the pipette box and puts the pipette box and the bottle cap box into the conveyor belt 5 for conveying.

Claims (6)

1. The intelligent detection equipment for the microbial limit is characterized by comprising an ISO 5-level laminar flow device (15), wherein a liquid suction device is arranged below the ISO 5-level laminar flow device (15), a conveyor belt (5) is arranged below the liquid suction device, a No. 1 robot (7) and a No. 2 robot (14) are arranged on two sides of the conveyor belt (5), and a dry-type heating constant-temperature device (12) and an automatic liquid suction pipe inlet and outlet device (13) are arranged on one side of the No. 2 robot (14); a culture dish storage device (6), a water bath (8) and a waste box (9) are sequentially arranged on one side of the No. 1 robot (7).

2. The intelligent detection equipment for the microbial limit as claimed in claim 1, wherein the ISO 5-level laminar flow device (15) comprises an air supply fan, a primary filter, a medium-efficiency filter and a high-efficiency filter which are sequentially arranged, and a differential pressure gauge and an anemometer are arranged in the ISO 5-level laminar flow device (15).

3. The apparatus according to claim 1, wherein the dry-type heating thermostat comprises a heating plate and a temperature control unit connected to the heating plate.

4. The intelligent detection device for the microbial limit according to claim 1, wherein the water bath (8) comprises a container, a heating coil is arranged at the bottom of the container, the heating coil is connected with a water bath circulation pipeline outside the container, and the water bath circulation pipeline is connected with the temperature control unit through a water pump.

5. The intelligent microbial limit detection device according to claim 1, wherein a No. 1 robot distribution box (10) and a No. 2 robot distribution box (11) connected with a No. 1 robot (7) and a No. 2 robot (14) are respectively arranged below the conveyor belt (5).

6. An intelligent microbial limit detection apparatus as claimed in claim 1 further comprising an automatic uncapping mechanism (16).

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