CN206624885U - The module mechanism of PCR gene amplification instrument - Google Patents
The module mechanism of PCR gene amplification instrument Download PDFInfo
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- CN206624885U CN206624885U CN201720318540.3U CN201720318540U CN206624885U CN 206624885 U CN206624885 U CN 206624885U CN 201720318540 U CN201720318540 U CN 201720318540U CN 206624885 U CN206624885 U CN 206624885U
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Abstract
It the utility model is related to gene-amplificative instrament technical field, there is provided a kind of module mechanism of PCR gene amplification instrument.The mechanism includes pedestal, radiator, heater, temperature sensor and control unit, the top of pedestal is provided with the sample cell for holding test tubes, filled with liquid metal in sample cell, the side of pedestal offers mounting hole and annular groove, mounting hole site is embedded in annular groove in the top of annular groove, heater, and temperature sensor is plugged in mounting hole, the bottom of radiator and pedestal connects, and radiator, heater and temperature sensor electrically connect with control unit.The utility model in sample cell by filling liquid metal, make to be full of liquid metal in the gap between test tube and sample cell, efficiently solve edge effect, it ensure that test tube temperature and the uniformity of base-plate temp, the speed of heating and cooling and the uniformity of test tube temperature are improved, greatly improved the precision and stability of the overall gene magnification effect of instrument and detection performance.
Description
Technical field
It the utility model is related to gene-amplificative instrament technical field, and in particular to a kind of module mechanism of PCR gene amplification instrument.
Background technology
PCR (Polymerase Chain Reaction, hereinafter referred to as PCR) is the mid-80 development
The isothermal DNA amplification to get up, have high specificity, susceptibility height, yield high, fast and convenient, reproducible, easily automatic
The advantages that change.In the equipment of temperature needed for each stage when PCR gene amplification instrument is a kind of simulation gene magnification for offer, and
Increase the function such as low-temperature storage and fluoroscopic examination, learned suitable for human genome project, medical jurisprudence, oncology, tissue and colony
The polymerization in the clinical diagnosis such as the research fields such as biology, paleontology, zoology, botany and virus, tumour, hereditary disease field
Enzyme chain reaction fluorogenic quantitative detection.
PCR is mainly included the following steps that：It is the first step, high-temperature denatured in 95 DEG C of lower template DNA；Second step, the mould at 60 DEG C
Plate DNA and primer process annealing renaturation；3rd step, primer medium temperature extends at 72 DEG C；Said process will after circulating more than 30 times
Reach DNA amplification purpose.
PCR gene amplification instrument in the market, include and write the main frame of above-mentioned each phase temperature control program and put down
Platform, then give the module mechanism with refrigeration heating chip to carry out power output by host platform, make the test tube on module mechanism
Groove surfaces temperature reaches the temperature needed for gene magnification, by equipped with the examination for needing the test tube of amplifing reagent to insert on module mechanism
Expanded in pipe groove, then analyzed by exciting and absorbing fluorescence data for photoelectric probe, so as to draw various judgements
Result.Wherein, the module mechanism of PCR gene amplification instrument is one of key element of PCR gene amplification instrument, determines PCR bases
Gene-amplification instrument overall gene magnification effect and detection performance precision and stability.
The module mechanism of existing PCR gene amplification instrument is typically plugged by the way that alternating temperature metal module is heated or cooled to change
In test tube temperature thereon, and then to realize amplification, due to alternating temperature metal module and test tube be difficult accomplish it is seamless contact, therefore
Can there are edge effect, poor temperature uniformity and the relatively low phenomenon of temperature rate.
The content of the invention
A kind of module mechanism of PCR gene amplification instrument provided by the utility model, solves and edge in the prior art be present
The problem of effect, poor temperature uniformity, low temperature rate.
To solve the above problems, the utility model provides a kind of module mechanism of PCR gene amplification instrument, the mechanism includes
Pedestal, radiator, heater, temperature sensor and control unit, the top of the pedestal are provided with the sample for holding test tubes
Groove, mounting hole and annular groove, the mounting hole site are offered filled with liquid metal, the side of the pedestal in the sample cell
In the top of the annular groove, the heater is embedded in the annular groove, and the temperature sensor is plugged in described
In mounting hole, the radiator is connected with the bottom of the pedestal, the radiator, the heater and the temperature sensor
Electrically connected with described control unit.
Wherein, in addition to located at the thermally conductive sheet between the pedestal and the radiator.
Wherein, the material of the thermally conductive sheet is liquid metal or heat-conducting silicone grease.
Wherein, the liquid metal includes at least two in bismuth, indium, tin, zinc, gold and silver.
Wherein, in addition to lid is located at the mat insulation at the top of the pedestal, and the mat insulation is provided with multiple for plugging examination
The first through hole of pipe.
Wherein, in addition to lid is located at the base cover on the mat insulation, and the base cover is provided with multiple second through holes, institute
The second through hole is stated to correspond with the first through hole.
Wherein, the quantity of the sample cell is multiple that the sample cell corresponds with the first through hole.
Wherein, the shape of the sample cell and the shape of test tube are adapted.
Wherein, the sample cell is located at the underface of multiple first through hole, the cross-sectional area of the sample cell with it is more
The individual first through hole, which is enclosed, to be set the area in the region to be formed and is adapted.
Wherein, in addition to lid is located at the pedestal cover plate on the sample cell, and the pedestal cover plate is provided with multiple threeways
Hole, the third through-hole correspond with the first through hole.
The utility model is simple in construction, cost is low, simple operation, by filling liquid metal in sample cell, makes test tube
Liquid metal is full of in gap between sample cell, efficiently solves edge effect, ensure that test tube temperature and pedestal temperature
The uniformity of degree, the speed of heating and cooling and the uniformity of test tube temperature are improved, greatly improved the overall gene of instrument and expand
Synergy fruit and the precision and stability of detection performance.
Brief description of the drawings
Fig. 1 is the top view of the module mechanism of PCR gene amplification instrument in the utility model embodiment；
Fig. 2 is sectional views of the Fig. 1 along A-A directions in the utility model embodiment 1；
Fig. 3 is sectional views of the Fig. 1 along B-B directions in the utility model embodiment 1；
Fig. 4 is sectional views of the Fig. 1 along A-A directions in the utility model embodiment 2.
Description of reference numerals：
1st, pedestal；1-1, sample cell；1-2, mounting hole；1-3, annular groove；
2nd, heater；3rd, radiator；4th, base cover；5th, mat insulation；
6th, temperature sensor；7th, thermally conductive sheet；8th, liquid metal；9th, test tube；
10th, control unit；11st, pedestal cover plate.
Embodiment
To make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with the utility model
Accompanying drawing, the technical scheme in the utility model is explicitly described, it is clear that described embodiment is the utility model one
Section Example, rather than whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not having
There is the every other embodiment made and obtained under the premise of creative work, belong to the scope of the utility model protection.
In description of the present utility model, unless otherwise indicated, " multiple " are meant that two or more.Term
The orientation or position of the instruction such as " on ", " under ", "left", "right", " interior ", " outer ", " front end ", " rear end ", " head ", " afterbody " are closed
It is to be described based on orientation shown in the drawings or position relationship, to be for only for ease of description the utility model and simplifying, rather than
Indicate or imply that signified device or element there must be specific orientation, with specific azimuth configuration and operation, therefore can not
It is interpreted as to limitation of the present utility model.
Embodiment 1
As shown in Figure 1 to Figure 3, the utility model embodiment provides a kind of module mechanism of PCR gene amplification instrument, the machine
Structure includes pedestal 1, radiator 3, heater 2, temperature sensor 6 and control unit 10, and the top of the pedestal 1, which is provided with, to be used to put
Put in the sample cell 1-1, the sample cell 1-1 of test tube 9 and offer mounting hole 1- filled with liquid metal 8, the side of the pedestal 1
2 and annular groove 1-3, the mounting hole 1-2 be located at the top of the annular groove 1-3, the heater 2 is embedded in the ring
In connected in star 1-3, the temperature sensor 6 is plugged in the mounting hole 1-2, the bottom of the radiator 3 and the pedestal 1
Connection, the radiator 3, the heater 2 and the temperature sensor 6 electrically connect with described control unit 10.
First, test tube 9 is inserted in sample cell 1-1, because liquid metal 8 has mobility, liquid metal 8 can quickly fill
Full gap between test tube 9 and sample cell 1-1, one layer of film of liquid metal 8 is formed in the outer wall of test tube 9, along with liquid metal 8
Good thermal conductivity, this layer of film of liquid metal 8 are equivalent to establish the heat transfer path between sample cell 1-1 and test tube 9, can be achieved
The quick transmission of heat between sample cell 1-1 and test tube 9, so can ensure pedestal 1 temperature and test tube 9 temperature substantially all the time
It is consistent；Then, the temperature that temperature sensor 6 monitors pedestal 1 in real time is the temperature of test tube 9, and the temperature information is sent
To control unit 10, control unit 10 controls radiating according to predetermined temperature control program and the temperature information got
The action of device 3 and heater 2, and then the temperature of pedestal 1 is adjusted, wait to expand to ensure that sample cell 1-1 temperature meets in test tube 9
Increase the temperature requirements of gene.Specifically, when test tube 9 needs heating, control unit 10 starts heater 2, and is passed according to temperature
The feedback information of sensor 6 adjusts the power of heater 2 in real time；When test tube 9 needs cooling, control unit 10 starts radiator 3, and
Adjust the power of radiator 3 in real time according to the feedback information of temperature sensor 6.
Preferably, in addition to located at the end face of thermally conductive sheet 7, i.e. thermally conductive sheet 7 between the pedestal 1 and the radiator 3
With together with the bottom surface pressing of pedestal 1, the bottom surface of thermally conductive sheet 7 is together with the end face pressing of radiator 3.So setting to reduce
Thermal resistance between radiator 3 and pedestal 1, so as to can further improve the rate of temperature fall of pedestal 1.
It is highly preferred that the material of the thermally conductive sheet 7 is liquid metal 8 or heat-conducting silicone grease.Due to the bottom surface of pedestal 1 and radiator
3 end faces all have certain roughness, therefore space appearance is had when contacting with each other, and the air in these spaces is the bad of heat
Conductor, the heat of pedestal 1 can be hindered to be conducted to radiator 3.And liquid metal 8 and heat-conducting silicone grease are respectively provided with good thermal conductivity,
It is empty that liquid metal 8 or heat-conducting silicone grease positioned at upper and lower surface in the presence of pedestal 1 and the pressure at both sides of radiator 3 can insert these
Gap so that the conduction of heat is more smooth.
Wherein, the liquid metal 8 includes at least two in bismuth, indium, tin, zinc, gold and silver.
In addition, also including the mat insulation 5 that lid is located at the top of pedestal 1, the mat insulation 5 is provided with multiple for plugging
The first through hole of test tube 9.By setting mat insulation 5 to reduce influence of the ambient temperature to the head temperature of pedestal 1, base can be made
The temperature of seat 1 is more synchronous with default temperature in control unit 10.For example, when heater 2 heats to pedestal 1, it is extraneous
Environment temperature is less than the temperature of pedestal 1, can greatly reduce the upper surface of pedestal 1 outwardly environment by the heat-blocking action of mat insulation 5
The heat of transmission.Wherein, the material of mat insulation 5 can be, but not limited to be PEEK (Polyetheretherketone) or PA66
(Polyamide 66) etc., as long as heat-insulated effect can be played.
Further, in addition to lid is located at the base cover 4 on the mat insulation 5, and the base cover 4 is provided with multiple second
Through hole, second through hole and the first through hole correspond the i.e. not only quantity phase of the quantity of the second through hole and first through hole
Together, and each second through hole is respectively positioned on the surface of corresponding first through hole.So setting is advantageous in that：On the one hand, may be used
Influence of the ambient temperature to the head temperature of pedestal 1 is further reduced, makes the temperature and 10 default temperature of control unit of pedestal 1
The temperature spent in control program is more synchronous；On the other hand, also compressible mat insulation 5, makes mat insulation 5 be close on pedestal 1, to subtract
Gap between small mat insulation 5 and pedestal 1 contact surface.Wherein, the material of base cover 4 can be, but not limited to be PEEK
(Polyetheretherketone) or PA66 (Polyamide 66) etc., as long as heat-insulated effect can be played.
Preferably, the quantity of the sample cell 1-1 is multiple that the sample cell 1-1 corresponds with the first through hole
I.e. not only sample cell 1-1 quantity and the quantity of first through hole are identical, and each sample cell 1-1 is respectively positioned on corresponding first and led to
The underface in hole.It is highly preferred that the shape of the sample cell 1-1 and the shape of test tube 9 are adapted i.e. test tube 9 and are placed on sample cell
It will not topple in 1-1.In use, the liquid metal 8 of 0.1ml molten conditions is added in each sample cell 1-1 to be filled
Full gap between test tube 9 and sample cell 1-1.When wherein, according to the liquid metal 8 that melting range is 50-60 DEG C, pedestal 1
The speed of heating and cooling can reach 10-15 DEG C/sec, and the temperature difference between each test tube 9 is can be controlled within 0.5 DEG C；According to fusing point
When scope is 60-75 DEG C of liquid metal 8, the speed of the heating and cooling of pedestal 1 can reach 15-20 DEG C/sec, the temperature between each test tube 9
Degree difference is can be controlled within 0.1 DEG C.In addition, in order to improve the precision of temperature monitoring, can be simultaneously in sample segment groove 1-1 bottom
Temperature sensor 6 is set, and wherein temperature sensor 6 is preferably the occasionally T-shaped thermocouple of K-type thermoelectricity.
Embodiment 2：
As shown in figure 4, the present embodiment is substantially the same manner as Example 1, for part the present embodiment same as Example 1 not
Repeat again, its difference is：In the present embodiment, the sample cell 1-1 is located at the underface of multiple first through hole, institute
State sample cell 1-1 cross-sectional area and multiple first through hole are enclosed and set the area in the region to be formed and be adapted, i.e., multiple first
The projection of through hole can be fallen into sample cell 1-1.So setting is advantageous in that：On the one hand, multiple test tubes 9 are placed on same
In sample cell 1-1, it can further reduce temperature error and edge effect between test tube 9；On the other hand, during 9 specification difference of test tube,
Mat insulation 5 and base cover 4 only need to be replaced, without changing pedestal 1, and then material and cost can be greatlyd save.
Preferably, in addition to lid is located at the pedestal cover plate 11 on the sample cell 1-1, and the pedestal cover plate 11 is provided with more
Individual third through-hole, the third through-hole and the first through hole correspond the i.e. not only quantity of third through-hole and first through hole
Quantity is identical, and each third through-hole is respectively positioned on the surface of corresponding first through hole.In order to avoid pedestal cover plate 11 is installed
Shi Fasheng misplaces, and can will be arranged to step surface at the top of sample cell 1-1 side walls, the edge phase of the step surface and pedestal cover plate 11
Match somebody with somebody.
Finally it should be noted that：Above example is only to illustrate the technical solution of the utility model, rather than its limitations；
Although the utility model is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：
It can still modify to the technical scheme described in foregoing embodiments, or which part technical characteristic is carried out etc.
With replacement；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the utility model technology
The spirit and scope of scheme.
Claims (10)
1. a kind of module mechanism of PCR gene amplification instrument, it is characterised in that including pedestal, radiator, heater, TEMP
Device and control unit, the top of the pedestal are provided with the sample cell for holding test tubes, filled with liquid metal in the sample cell,
The side of the pedestal offers mounting hole and annular groove, and the mounting hole site is described to add in the top of the annular groove
Hot device is embedded in the annular groove, and the temperature sensor is plugged in the mounting hole, the radiator and the base
The bottom connection of seat, the radiator, the heater and the temperature sensor electrically connect with described control unit.
2. the module mechanism of PCR gene amplification instrument according to claim 1, it is characterised in that also include being located at the base
Thermally conductive sheet between seat and the radiator.
3. the module mechanism of PCR gene amplification instrument according to claim 2, it is characterised in that the material of the thermally conductive sheet
For liquid metal or heat-conducting silicone grease.
4. the module mechanism of PCR gene amplification instrument according to claim 1, it is characterised in that the liquid metal includes
At least two in bismuth, indium, tin, zinc, gold and silver.
5. the module mechanism of the PCR gene amplification instrument according to any one of Claims 1-4, it is characterised in that also include lid
The mat insulation being located at the top of the pedestal, the mat insulation are provided with multiple first through hole for being used to plug test tube.
6. the module mechanism of PCR gene amplification instrument according to claim 5, it is characterised in that be also located at including lid described
Base cover on mat insulation, the base cover are provided with multiple second through holes, and second through hole and the first through hole are one by one
It is corresponding.
7. the module mechanism of PCR gene amplification instrument according to claim 6, it is characterised in that the quantity of the sample cell
To be multiple, the sample cell corresponds with the first through hole.
8. the module mechanism of PCR gene amplification instrument according to claim 7, it is characterised in that the shape of the sample cell
It is adapted with the shape of test tube.
9. the module mechanism of PCR gene amplification instrument according to claim 6, it is characterised in that the sample cell is positioned at more
The underface of the individual first through hole, the cross-sectional area of the sample cell encloses with multiple first through hole sets the region to be formed
Area is adapted.
10. the module mechanism of PCR gene amplification instrument according to claim 9, it is characterised in that be also located at including lid described
Pedestal cover plate on sample cell, the pedestal cover plate are provided with multiple third through-holes, the third through-hole and the first through hole
Correspond.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720318540.3U CN206624885U (en) | 2017-03-29 | 2017-03-29 | The module mechanism of PCR gene amplification instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720318540.3U CN206624885U (en) | 2017-03-29 | 2017-03-29 | The module mechanism of PCR gene amplification instrument |
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| CN206624885U true CN206624885U (en) | 2017-11-10 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020155607A1 (en) * | 2019-01-29 | 2020-08-06 | 莫纳（苏州）生物科技有限公司 | Pcr base and pcr device |
| CN111793679A (en) * | 2020-08-12 | 2020-10-20 | 济南国益生物科技有限公司 | Method and device for reducing evaporation and non-specific reaction in PCR amplification |
| CN113373043A (en) * | 2021-06-09 | 2021-09-10 | 北京卓诚惠生生物科技股份有限公司 | Thermal cracking temperature control device |
| CN115364918A (en) * | 2022-09-05 | 2022-11-22 | 鲲鹏基因（北京）科技有限责任公司 | Polymerase chain reaction analyzer temperature control device based on metal solid-liquid conversion |
-
2017
- 2017-03-29 CN CN201720318540.3U patent/CN206624885U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020155607A1 (en) * | 2019-01-29 | 2020-08-06 | 莫纳（苏州）生物科技有限公司 | Pcr base and pcr device |
| CN111793679A (en) * | 2020-08-12 | 2020-10-20 | 济南国益生物科技有限公司 | Method and device for reducing evaporation and non-specific reaction in PCR amplification |
| CN113373043A (en) * | 2021-06-09 | 2021-09-10 | 北京卓诚惠生生物科技股份有限公司 | Thermal cracking temperature control device |
| CN115364918A (en) * | 2022-09-05 | 2022-11-22 | 鲲鹏基因（北京）科技有限责任公司 | Polymerase chain reaction analyzer temperature control device based on metal solid-liquid conversion |
| CN115364918B (en) * | 2022-09-05 | 2023-04-14 | 鲲鹏基因（北京）科技有限责任公司 | Polymerase chain reaction analyzer temperature control device based on metal solid-liquid conversion |
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