CN215856127U - Thermal cycle structure of PCR amplification platform - Google Patents
Thermal cycle structure of PCR amplification platform Download PDFInfo
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- CN215856127U CN215856127U CN202121958274.3U CN202121958274U CN215856127U CN 215856127 U CN215856127 U CN 215856127U CN 202121958274 U CN202121958274 U CN 202121958274U CN 215856127 U CN215856127 U CN 215856127U
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Abstract
The utility model relates to the technical field of nucleic acid detection, in particular to a thermal cycle structure of a PCR amplification platform, which comprises a reaction tube bracket, wherein a thermal cycle block is arranged below the reaction tube bracket, the thermal cycle block comprises a plurality of heating units arranged in an array manner, and each heating unit comprises a fixed block, a heat insulator, an outer fixed plate, a Peltier and a heating block; two sides of the fixed block are provided with first grooves, the heat insulation body is arranged in the first grooves, the heating block is arranged in the heat insulation body, and the heating block is provided with a plurality of inserting holes; the Peltier is arranged close to the heating block, and the outer fixing plate is arranged on the outer side of the heat insulator. The utility model can rapidly and uniformly heat the reaction tube and can rapidly cool the heating unit; meanwhile, the temperature detection and thermal protection functions are achieved.
Description
Technical Field
The utility model relates to the technical field of nucleic acid detection, in particular to a thermal cycle structure of a PCR amplification platform.
Background
Nucleic acid extraction is an essential link in various molecular biology experiments, and almost every laboratory requires nucleic acid extraction for separation and purification of biomolecules. The nucleic acid extractor is an instrument which automatically finishes nucleic acid extraction work by using a matched nucleic acid extraction reagent, and is widely applied to the fields of clinical disease diagnosis, microbiological detection and the like; after nucleic acid extraction, PCR detection, which is a molecular biological technique for amplifying specific DNA fragments, is required. With the development of nucleic acid detection technology, the integrated nucleic acid detection and amplification machine gradually develops towards automation and simplification.
The PCR amplification needs to go through three stages, namely a deformation stage, an annealing stage and an extension stage, each stage needs different heating conditions, which requires that the PCR amplification platform has a precise heating device with uniform heating.
Chinese patent CN209652329U discloses a heating device for PCR amplification apparatus, which comprises: a heating frame including a heating frame upper surface, a heating frame lower surface, and a heating frame body extending between the heating frame upper surface and the heating frame lower surface; a heating element; heating the container by a PCR tube; and a hollow liquid flow passage disposed within the heating frame body, the hollow liquid flow passage including at least one liquid inlet and at least one liquid outlet. The rapid cooling of the PCR amplification instrument can be realized by utilizing liquid circulation. When the heating device of the PCR amplification instrument is in a heating state, the liquid does not flow, and uniform heating can be realized.
In the heating device of the PCR amplification instrument in the technology, heat is transferred to the surrounding by the heat of the heating element for heating, the temperature around the heating element is easy to be higher, and the temperature of the whole PCR amplification platform is difficult to realize uniformity. Therefore, it is highly desirable to design a thermal cycling structure of a PCR amplification platform to achieve uniform heating of the device.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems in the prior art, the utility model provides a thermal cycling structure of a PCR amplification platform, which comprises the following technical scheme:
a thermal cycle structure of a PCR amplification platform comprises a reaction tube bracket, wherein a thermal cycle block is arranged below the reaction tube bracket, the thermal cycle block comprises a plurality of heating units arranged in an array, and each heating unit comprises a fixed block, a heat insulator, an outer fixed plate, a Peltier and a heating block; two sides of the fixed block are provided with first grooves, the heat insulation body is arranged in the first grooves, the heating block is arranged in the heat insulation body, and the heating block is provided with a plurality of inserting holes; the Peltier is arranged close to the heating block, and the outer fixing plate is arranged on the outer side of the heat insulator.
Further, the heating unit further comprises a temperature sensor, a temperature detection hole is formed in the heating block, and the temperature sensor is arranged in the temperature detection hole.
Furthermore, the heating unit further comprises a circuit adapter plate and a thermal protector, wherein the circuit adapter plate is arranged above the fixing block; the right side of the heat insulator is provided with a right groove, the thermal protector is arranged in the right groove, and the thermal protector is electrically connected with the circuit adapter plate.
Furthermore, the peltier is arranged on two sides of the heating block, the hot end of the peltier is in contact with the heating block, the cold end of the peltier on the outer side of the heating block is in contact with the outer fixing plate, and the cold end of the peltier on the inner side of the heating block is in contact with the fixing block.
Furthermore, an upper cover plate is arranged above the thermal cycle block, and a plurality of through holes corresponding to the inserting holes are formed in the upper cover plate.
Further, a water sump is arranged below the thermal cycle block, and a water sump bottom cover is arranged below the water sump; a water inlet joint is arranged on one side of the water bin, a water outlet joint is arranged on the other side of the water bin, the water bin is provided with an upper surface and a lower surface, and the lower surface is provided with a snake-shaped groove; the water bin bottom cover is provided with a snake-shaped protrusion corresponding to the snake-shaped groove, and the snake-shaped protrusion is embedded into the snake-shaped groove to form a closed snake-shaped water channel.
Furthermore, a third groove is formed in the upper surface of the water bin, and the heat circulation block is embedded into the third groove.
The utility model also provides a PCR amplification instrument, which comprises the thermal cycle structure.
The utility model has the beneficial effects that:
1. according to the utility model, the thermal cycle block is arranged below the PCR amplification platform and comprises a plurality of heating units, each heating unit comprises a plurality of Peltier devices, the water sump and the water sump bottom cover are arranged below the thermal cycle block, and the water sump bottom cover are connected to form a closed snake-shaped water channel; when amplification is carried out, the Peltier patches on the two sides of the heating block are used for heating the heating block, so that the reaction tube is heated; the working temperature of the thermal circulation block can be quickly homogenized under the action of water flow of the snake-shaped water channel; after amplification is finished, heat can be rapidly transmitted out by utilizing water flow in the snake-shaped water channel, so that the heating unit is rapidly cooled.
2. The working temperature of the heating unit is detected in real time through the temperature sensor arranged on the fixed block, the real-time temperature is fed back to the equipment control center, if the working temperature of the heating unit is abnormal, the thermal protector is automatically switched off, the Peltier stops heating, and when the working temperature of the heating unit is recovered to be normal, the thermal protector is automatically switched on, the Peltier is reheated, and the equipment normally operates.
Drawings
FIG. 1 is an exploded view of the present invention.
Fig. 2 is an exploded structural view of the heating unit.
Fig. 3 is a schematic structural view of the water sump.
Description of reference numerals:
1. the device comprises a reaction tube support, 2, a heating unit, 3, a fixing block, 4, a heat insulator, 5, an outer fixing plate, 6, a Peltier, 7, a heating block, 8, a temperature sensor, 9, a circuit adapter plate, 10, a thermal protector, 11, an upper cover plate, 12, a water bin, 13, a water bin bottom cover, 14, a water inlet connector, 15 and a water outlet connector.
Detailed Description
The technical solutions of the present invention will be described in detail with reference to the accompanying drawings, and it is obvious that the described embodiments are not all embodiments of the present invention, and all other embodiments obtained by those skilled in the art without any inventive work belong to the protection scope of the present invention.
It is to be understood that the terms "center," "upper," "lower," "horizontal," "left," "right," "lateral," "longitudinal," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the present invention and for the simplicity of description, and do not indicate or imply that the referenced devices or elements must be in a particular orientation, constructed and operated in a particular orientation, and thus are not to be considered as limiting the present invention.
As shown in fig. 1-3, the present invention provides a thermal cycling structure of a PCR amplification platform, which comprises a reaction tube support 1, wherein a thermal cycling block is arranged below the reaction tube support 1, the thermal cycling block comprises a plurality of heating units 2 arranged in an array, and the heating units 2 comprise a fixed block 3, a heat insulator 4, an external fixed plate 5, a peltier 6 and a heating block 7; rectangular first grooves are symmetrically formed in two sides of the fixed block 3; the heat preservation body 4 is arranged in the first groove, the heat preservation body 4 is of a U-shaped structure, a left groove is formed in the left side of the heat preservation body 4, and a right groove is formed in the right side of the heat preservation body 4; the heating block 7 is arranged in the heat insulator 4, a plurality of inserting holes are formed in the heating block 7, and the left groove and the inserting holes are used for placing reaction tubes;
two pieces of the Peltier 6 are arranged on two sides of the heating block 7, the Peltier 6 and the heating block 7 are all embedded into the heat insulation body 4, and the heat insulation body 4 is all embedded into the first groove; the outer fixing plate 5 is arranged on the outer side of the heat insulator 4, and the length of the outer fixing plate 5 is the same as that of the fixing block 3; the hot end of the Peltier 6 is in contact with the heating block 7, the cold end of the Peltier 6 on the outer side of the heating block 7 is in contact with the outer fixing plate 5, and the cold end of the Peltier 6 on the inner side of the heating block 7 is in contact with the fixing block 3.
The heating unit 2 further comprises a temperature sensor 8, a temperature detection hole is formed in the heating block 7, and the temperature sensor 8 is arranged in the temperature detection hole.
Specifically, the heating unit 2 further comprises a circuit adapter plate 9 and a thermal protector 10, wherein the circuit adapter plate 9 is arranged above the fixing block 3; the thermal protector 10 is arranged in the right groove, and the thermal protector 10 is electrically connected with the circuit adapter plate 9.
Specifically, an upper cover plate 11 is arranged above the thermal cycle block, a plurality of through holes corresponding to the inserting holes are formed in the upper cover plate 11, and the upper cover plate 11 is used for preserving heat of the heating unit 2 and preventing liquid in the reaction tube from spilling.
Specifically, a water sump 12 is arranged below the heat circulation block, and a water sump bottom cover 13 is arranged below the water sump 12; a water inlet joint 14 is arranged on one side of the water bin 12, a water outlet joint 15 is arranged on the other side of the water bin 12, the water bin 12 is provided with an upper surface and a lower surface, a third groove is arranged on the upper surface of the water bin 12, and the heat circulation block is embedded into the third groove; the lower surface sets up snakelike recess, set up on the sump bottom cover 13 with snakelike arch that snakelike recess corresponds, snakelike protruding embedding snakelike recess forms confined snakelike water course.
Before amplification, a reaction tube is placed on the reaction tube bracket 1, and the bottom of the reaction tube penetrates through the upper cover plate 11 and extends into the plug hole.
When amplification is carried out, the Peltier 6 is electrified to work, the Peltier 6 generates heat by utilizing the Peltier effect and transfers the heat to the reaction tube through the heating block 7, and then the sample liquid in the reaction tube is heated; meanwhile, the cooling liquid enters the snake-shaped water channel through the water inlet joint 14 and then flows out of the water outlet joint 15, and takes away heat generated by the heat circulation block, so that the working temperature of the heating unit 2 is kept within a preset temperature range, and the working temperature of the heating unit 2 is quickly homogenized.
When the amplification is finished, the circuit of the Peltier 6 is disconnected, the heating unit 2 stops heating, and at the moment, the cooling liquid in the water channel can rapidly take away heat, so that the rapid cooling of the heating unit 2 is realized.
The utility model also provides a PCR amplification instrument, which comprises the thermal cycle structure.
Although the present invention has been described in detail with reference to examples, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the scope of the present invention.
Claims (8)
1. The utility model provides a thermal cycle structure of PCR amplification platform, includes the reaction tube support, the below of reaction tube support sets up the thermal cycle piece, the thermal cycle piece includes the heating unit that a plurality of arrays set up, its characterized in that: the heating unit comprises a fixed block, a heat insulator, an outer fixed plate, a Peltier and a heating block; two sides of the fixed block are provided with first grooves, the heat insulation body is arranged in the first grooves, the heating block is arranged in the heat insulation body, and the heating block is provided with a plurality of inserting holes; the Peltier is arranged close to the heating block, and the outer fixing plate is arranged on the outer side of the heat insulator.
2. The thermal cycle structure of claim 1, wherein: the heating unit further comprises a temperature sensor, a temperature detection hole is formed in the heating block, and the temperature sensor is arranged in the temperature detection hole.
3. The thermal cycle structure of claim 2, wherein: the heating unit further comprises a circuit adapter plate and a thermal protector, and the circuit adapter plate is arranged above the fixing block; the right side of the heat insulator is provided with a right groove, the thermal protector is arranged in the right groove, and the thermal protector is electrically connected with the circuit adapter plate.
4. The thermal cycle structure of claim 1, wherein: the two sides of the heating block are respectively provided with the Peltier, the hot end of the Peltier is in contact with the heating block, the cold end of the Peltier on the outer side of the heating block is in contact with the outer fixing plate, and the cold end of the Peltier on the inner side of the heating block is in contact with the fixing block.
5. The thermal cycle structure of claim 1, wherein: an upper cover plate is arranged above the thermal cycle block, and a plurality of through holes corresponding to the inserting holes are formed in the upper cover plate.
6. The thermal cycle structure of claim 1, wherein: a water sump is arranged below the thermal circulation block, and a water sump bottom cover is arranged below the water sump; a water inlet joint is arranged on one side of the water bin, a water outlet joint is arranged on the other side of the water bin, the water bin is provided with an upper surface and a lower surface, and the lower surface is provided with a snake-shaped groove; the water bin bottom cover is provided with a snake-shaped protrusion corresponding to the snake-shaped groove, and the snake-shaped protrusion is embedded into the snake-shaped groove to form a closed snake-shaped water channel.
7. The thermal cycle structure of claim 6, wherein: the upper surface of sump sets up the third recess, the thermal cycle piece imbeds in the third recess.
8. A PCR amplifier comprising the thermal cycling structure according to any one of claims 1-7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121958274.3U CN215856127U (en) | 2021-08-19 | 2021-08-19 | Thermal cycle structure of PCR amplification platform |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121958274.3U CN215856127U (en) | 2021-08-19 | 2021-08-19 | Thermal cycle structure of PCR amplification platform |
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| CN215856127U true CN215856127U (en) | 2022-02-18 |
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| CN202121958274.3U Active CN215856127U (en) | 2021-08-19 | 2021-08-19 | Thermal cycle structure of PCR amplification platform |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118170190A (en) * | 2024-05-15 | 2024-06-11 | 苏州百源基因技术有限公司 | Temperature control system of PCR instrument |
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2021
- 2021-08-19 CN CN202121958274.3U patent/CN215856127U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118170190A (en) * | 2024-05-15 | 2024-06-11 | 苏州百源基因技术有限公司 | Temperature control system of PCR instrument |
| CN118170190B (en) * | 2024-05-15 | 2024-08-02 | 苏州百源基因技术有限公司 | Temperature control system of PCR instrument |
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