CN118170190B - Temperature control system of PCR instrument - Google Patents
Temperature control system of PCR instrument Download PDFInfo
- Publication number
- CN118170190B CN118170190B CN202410599370.5A CN202410599370A CN118170190B CN 118170190 B CN118170190 B CN 118170190B CN 202410599370 A CN202410599370 A CN 202410599370A CN 118170190 B CN118170190 B CN 118170190B
- Authority
- CN
- China
- Prior art keywords
- temperature
- peltier
- signal
- reaction chamber
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000006243 chemical reaction Methods 0.000 claims abstract description 188
- 230000002159 abnormal effect Effects 0.000 claims abstract description 53
- 238000000034 method Methods 0.000 claims abstract description 52
- 238000004364 calculation method Methods 0.000 claims abstract description 21
- 230000001276 controlling effect Effects 0.000 claims abstract description 13
- 230000001105 regulatory effect Effects 0.000 claims abstract description 10
- 238000012795 verification Methods 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims description 82
- 238000001816 cooling Methods 0.000 claims description 77
- 230000008859 change Effects 0.000 claims description 59
- 230000008569 process Effects 0.000 claims description 35
- 238000005057 refrigeration Methods 0.000 claims description 30
- 230000033228 biological regulation Effects 0.000 claims description 24
- 238000012544 monitoring process Methods 0.000 claims description 21
- 230000009467 reduction Effects 0.000 claims description 12
- 238000004458 analytical method Methods 0.000 claims description 10
- 230000000630 rising effect Effects 0.000 claims description 7
- 238000012935 Averaging Methods 0.000 claims description 6
- 230000000087 stabilizing effect Effects 0.000 claims description 6
- 230000005856 abnormality Effects 0.000 claims description 2
- 230000004044 response Effects 0.000 abstract description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 71
- 230000032683 aging Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000007363 regulatory process Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/30—Automatic controllers with an auxiliary heating device affecting the sensing element, e.g. for anticipating change of temperature
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
- Control Of Temperature (AREA)
Abstract
The invention discloses a temperature control system of a PCR instrument, which comprises: and a data acquisition module: the method comprises the steps of obtaining the point position of the Peltier of a reaction chamber in a PCR instrument, and obtaining the initial temperature value of the point position of the Peltier of the reaction chamber in the PCR instrument; and a data processing module: the method comprises the steps of performing standard deviation calculation on point position initial temperature values of the Peltier in a reaction chamber in a PCR instrument to obtain point position initial temperature fluctuation values of the Peltier in the reaction chamber; and a state verification module: for obtaining a normal signal or an abnormal signal; the work judging module is used for: for generating a temperature execution signal; the execution module: regulating and controlling the temperature of a reaction chamber of the PCR instrument based on the temperature execution signal; the temperature in the reaction chamber is controlled through the multi-point peltier, the response speed is high, meanwhile, the peltier state in the initial state is checked, the stability and the safety of the work of the PCR instrument are guaranteed, the abnormal condition of the peltier is timely found, and the work of the PCR instrument is prevented from being problematic.
Description
Technical Field
The invention relates to the technical field of PCR instruments, in particular to a temperature control system of a PCR instrument.
Background
The PCR amplification instrument is also called a PCR gene amplification instrument, a PCR nucleic acid amplification instrument and a polymerase chain reaction nucleic acid amplification instrument, is an instrument and equipment for amplifying specific DNA by utilizing the PCR (Polymerase chain reaction ) technology, and is widely applied to medical and biological laboratories;
PCR reaction is generally divided into three stages of denaturation, annealing and extension, and the specific reaction state is determined according to the reactions of enzymes adopted by different reagents;
When the existing PCR instrument performs temperature change control, the electric heating mode is adopted to realize low heat conversion efficiency, so that the temperature change speed is low, the precision is difficult to control, and the problem can be solved by adopting the Peltier to perform temperature adjustment; but in the temperature control process of the PCR instrument, the temperature in the reaction chamber is regulated and controlled by adopting multi-point Peltier, and the problem of insufficient precision still occurs in the regulation and control of the temperature, because the working state of the Peltier is influenced by current, the self property of the Peltier also determines the heat transfer effect, and in the working process, the situation of abnormal working of the Peltier is easy to occur, the working of the PCR instrument is influenced, and potential safety hazards are caused, so that the temperature control system of the PCR instrument is provided.
Disclosure of Invention
The invention aims to provide a temperature control system of a PCR instrument, which solves the problems in the background.
The aim of the invention can be achieved by the following technical scheme:
A temperature control system for a PCR instrument, comprising:
And a data acquisition module: the method comprises the steps of obtaining the point positions of the Peltier in a reaction chamber in a PCR instrument, and marking the point positions of the Peltier; acquiring a point location initial temperature value of a Peltier of a reaction chamber in a PCR instrument;
And a data processing module: the method comprises the steps of (1) carrying out standard deviation calculation on a point position initial temperature value TCS i of the peltier in a reaction chamber in a PCR instrument to obtain a point position initial temperature fluctuation value TCSB of the peltier in the reaction chamber;
and a state verification module: the method comprises the steps of acquiring a reaction chamber working temperature fluctuation threshold TPJy of a PCR instrument; comparing the initial temperature fluctuation value TCSB of the point position with a working temperature fluctuation threshold TPJy; and obtaining a normal signal or an abnormal signal;
the work judging module is used for: the method comprises the steps of obtaining an initial temperature value TFS and a target temperature value TBM of a reaction chamber in a PCR instrument; comparing the initial temperature TFS of the reaction chamber with a target temperature TBM; and generating a temperature execution signal, wherein the temperature execution signal comprises: a temperature increase signal, a temperature hold signal, and a temperature decrease signal;
The execution module: controlling the working mode of the Peltier based on the temperature execution signal; regulating and controlling the temperature of a reaction chamber of the PCR instrument; comprises a state monitoring unit and a regulation analysis unit.
As a further scheme of the invention: the state monitoring unit is used for carrying out abnormal monitoring on the working state in the temperature regulation and control process and obtaining a state signal; wherein the status signal comprises: a heat conduction stabilizing signal, a heat conduction abnormal signal, a cooling stabilizing signal and a cooling abnormal signal.
As a further scheme of the invention: the regulation and control analysis unit is used for analyzing the regulation and control data and obtaining a conversion signal; wherein converting the signal comprises: a heating conversion normal signal, a heating conversion abnormal signal, a cooling conversion normal signal, and a cooling conversion abnormal signal.
As a further scheme of the invention: the state verification module is characterized in that: comparing the point initial temperature fluctuation value TCSB with the working temperature fluctuation threshold TPJy;
If the point initial temperature fluctuation value TCSB is smaller than or equal to the working temperature fluctuation threshold TPJy; generating a normal signal;
If the point initial temperature fluctuation value TCSB is larger than the working temperature fluctuation threshold TPJy; an exception signal is generated.
As a further scheme of the invention: in the work judging module, comparing an initial temperature value TFS of the reaction chamber with a target temperature value TBM;
If the initial temperature TFS of the reaction chamber is smaller than the target temperature TBM, generating a temperature rise signal;
If the initial temperature value TFS of the reaction chamber is equal to the target temperature value TBM, generating a temperature maintaining signal;
if the initial temperature TFS of the reaction chamber is greater than the target temperature TBM, a temperature reduction signal is generated.
As a further scheme of the invention: the execution module regulates and controls the temperature of a reaction chamber of the PCR instrument based on the temperature execution signal, and comprises:
Based on the temperature rising signal, controlling the current direction of the peltier to enable the peltier to heat the reaction chamber; heating the reaction chamber of the PCR instrument;
based on the temperature reduction signal, controlling the current direction of the peltier to enable the peltier to be in a refrigeration mode for the reaction chamber; cooling the reaction chamber of the PCR instrument;
Based on the temperature maintaining signal, the Peltier is started to be in a standby state, and the temperature value of a reaction chamber in the PCR instrument is monitored by taking the unit time t as the interval duration.
As a further scheme of the invention: the state monitoring unit is used for monitoring the working state in the temperature regulation process abnormally, and comprises the following steps:
Dividing time nodes in heating time by taking unit time t as interval duration, and marking the time nodes as j, wherein j is 0, 1, 2 and 3 … …;
The temperature value of the reaction chamber at the moment of each time node j in the heating time is obtained and marked as TFZ j,
Calculating and obtaining a temperature change rate value TBV j of the reaction chamber at a time node j in heating time;
acquiring a point location temperature value of each Peltier at the moment of each time node j in heating time, and marking the point location temperature value as TCS （i,j）;
calculating and obtaining the point temperature change rate of the Peltier at the moment of the time node j in the heating time, and marking the point temperature change rate as TDV （i,j）;
summing and averaging the point position temperature change rates of the Peltier of the i point positions in the PCR instrument to obtain the point position temperature change average rate TDVJZ j of the Peltier at the moment of the time node j;
Calculating a temperature conduction ratio TCD j of the Peltier at the moment of the time node j;
if the temperature conduction ratio TCD j is greater than or equal to the temperature conduction threshold TCDy; generating a Peltier heat conduction stable signal;
If the temperature conduction ratio TCD j is less than the temperature conduction threshold TCDy; generating a Peltier heat conduction abnormal signal.
As a further scheme of the invention: the state monitoring unit is used for monitoring the working state in the temperature regulation process abnormally, and comprises the following steps:
dividing time nodes in the cooling time by taking unit time t as interval duration, and marking the time nodes as k, wherein k is 0, 1, 2 and 3 … …;
Obtaining the temperature value of the reaction chamber at the moment k of each time node in the cooling time, marking the temperature value as TXZ k,
Calculating and obtaining a reaction chamber temperature change rate value TXV k at a time node k in the cooling time;
Acquiring a point location temperature value of each Peltier at the moment of each time node k in cooling time, and marking the point location temperature value as TXS （i,k）;
calculating and obtaining the point temperature change rate of the Peltier at the moment of the time node k in the cooling time, and marking the point temperature change rate as TXDV （i,k）;
Summing and averaging the point position temperature change rates of the Peltier of the i point positions in the PCR instrument to obtain the point position temperature change average rate TDVXZ k of the Peltier at the moment of the time node k;
Calculating a cooling conduction ratio TXD k of the Peltier at the moment k of the time node;
if the cooling conduction ratio TXD k is greater than or equal to the cooling conduction threshold TXDy; generating a Peltier cooling stable signal;
If the cooling conduction ratio TXD k is less than the cooling conduction threshold TXDy; generating a Peltier cooling abnormal signal.
As a further scheme of the invention: the operation mode of the regulation and control analysis unit comprises the following steps:
Acquiring heating current values of Peltier at the moment of each time node j in heating time and marking the heating current values as IR j;
Carrying out standard deviation calculation on a heating current value IRj in heating time to obtain a heating current fluctuation value IRB;
Meanwhile, standard deviation calculation is carried out on point location temperature change average rate TDVJZ j of the Peltier in heating time, so as to obtain a point location temperature change rate fluctuation value TDVJZB;
By passing through Calculating to obtain a current heating conversion deviation value IZT;
Comparing the current heating conversion deviation value IZT with a current heating conversion deviation threshold IZTy;
if the current heating conversion deviation value IZT is less than or equal to the current heating conversion deviation threshold IZTy; generating a Peltier heating conversion normal signal;
If the current heating conversion deviation value IZT is greater than the current heating conversion deviation threshold IZTy; generating a Peltier heating conversion abnormal signal.
As a further scheme of the invention: the operation mode of the regulation and control analysis unit comprises the following steps:
Acquiring a cooling current value of the Peltier at the moment k of each time node in cooling time and marking the cooling current value as IL k;
standard deviation calculation is carried out on the cooling current value IL k in the cooling time to obtain a cooling current fluctuation value ILB;
meanwhile, standard deviation calculation is carried out on the point location temperature change average rate TDVXZ k of the Peltier in the cooling time to obtain a point location temperature change rate fluctuation value TDVXZB;
By passing through Calculating to obtain a current refrigeration conversion deviation value ILT;
comparing the current refrigeration conversion deviation value ILT with a current refrigeration conversion deviation threshold ILTy;
If the current refrigeration conversion deviation value ILT is smaller than or equal to the current refrigeration conversion deviation threshold ILTy; generating a Peltier refrigeration conversion normal signal;
if the current refrigeration conversion deviation value ILT is greater than or equal to the current refrigeration conversion deviation threshold ILTy; generating a Peltier refrigeration conversion abnormal signal.
The invention has the beneficial effects that:
According to the invention, the temperature in the reaction chamber is controlled through the multi-point peltier, the response speed is high, meanwhile, the peltier state in the initial state is checked, and the temperature deviation of the inner peltier is avoided when the temperature of the PCR instrument is restored to the room temperature after the continuous work, so that the measurement result of the reuse of the PCR instrument is influenced;
In addition, in the working process, through the real-time monitoring of the temperature of the reaction chamber and the temperature of the reaction chamber in the temperature rising process and the temperature reducing process, whether the working state of the peltier is abnormal or not can be timely found in the temperature regulating process, the working stability and safety of the PCR instrument are ensured, meanwhile, after the temperature regulating is finished, the influence of the current change in the temperature regulating process on the working state of the peltier can be analyzed, and further the working state of the peltier in the working process is judged to be changed according to the current change, so that whether the temperature regulating of the peltier is accurate or not can be found, the abnormal condition of the peltier can be timely found, and the problem of the working of the PCR instrument is avoided.
Drawings
The invention is further described below with reference to the accompanying drawings.
FIG. 1 is a block diagram of a system of the present invention;
FIG. 2 is a schematic diagram of an implementation module in the present invention;
fig. 3 is a schematic diagram of the method of operation of the system of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Referring to fig. 1 and 2, the present invention is a temperature control system of a PCR instrument, comprising:
And a data acquisition module: the method comprises the steps of obtaining the point positions of the Peltier in a reaction chamber in a PCR instrument, and marking the point positions of the Peltier; acquiring a point location initial temperature value of a Peltier of a reaction chamber in a PCR instrument;
And a data processing module: the method comprises the steps of (1) carrying out standard deviation calculation on a point position initial temperature value TCS i of the peltier in a reaction chamber in a PCR instrument to obtain a point position initial temperature fluctuation value TCSB of the peltier in the reaction chamber;
and a state verification module: the method comprises the steps of acquiring a reaction chamber working temperature fluctuation threshold TPJy of a PCR instrument; comparing the initial temperature fluctuation value TCSB of the point position with a working temperature fluctuation threshold TPJy; and obtaining a normal signal or an abnormal signal;
the work judging module is used for: the method comprises the steps of obtaining an initial temperature value TFS and a target temperature value TBM of a reaction chamber in a PCR instrument; comparing the initial temperature TFS of the reaction chamber with a target temperature TBM; and generating a temperature execution signal, wherein the temperature execution signal comprises: a temperature increase signal, a temperature hold signal, and a temperature decrease signal;
the execution module: controlling the working mode of the Peltier based on the temperature execution signal; regulating and controlling the temperature of a reaction chamber of the PCR instrument; comprises a state monitoring unit and a regulation analysis unit;
A state monitoring unit: the system is used for carrying out abnormal monitoring on the working state in the temperature regulation and control process and obtaining a state signal; wherein the status signal comprises: a heat conduction stabilizing signal, a heat conduction abnormal signal, a cooling stabilizing signal and a cooling abnormal signal;
Regulation and control analysis unit: the control data are used for analyzing the control data, and a conversion signal is obtained; wherein converting the signal comprises: a heating conversion normal signal, a heating conversion abnormal signal, a cooling conversion normal signal and a cooling conversion abnormal signal;
through the real-time monitoring to the reaction chamber temperature and the peltier temperature of the temperature rising process and the temperature reducing process of the reaction chamber, whether the working state of the peltier is abnormal or not can be timely found in the temperature regulation process, the working stability and the working safety of the PCR instrument are guaranteed, meanwhile, the influence of the current change in the temperature regulation process on the peltier working state can be analyzed after the temperature regulation is finished, and further the working state of the peltier in the working process is judged to be changed according to the current change, so that whether the temperature regulation of the peltier is accurate or not is found, the abnormal condition of the peltier is timely found, and the problem of the work of the PCR instrument is avoided.
Example two
Based on the temperature control system of the above embodiment, referring to fig. 3, the present embodiment provides a temperature control method of a PCR apparatus, including:
Step S01: acquiring the point position of the Peltier in a reaction chamber in a PCR instrument, and marking the point position of the Peltier as i, wherein i is 1, 2 and 3 … …;
step S02: acquiring a point position initial temperature value of a Peltier in a reaction chamber in a PCR instrument, and marking the point position initial temperature value as TCS i;
Performing standard deviation calculation on a point location initial temperature value TCS i of the Peltier in a reaction chamber in the PCR instrument to obtain a point location initial temperature fluctuation value TCSB of the Peltier in the reaction chamber;
Step S03: acquiring a reaction chamber working temperature fluctuation threshold TPJy of the PCR instrument;
comparing the point initial temperature fluctuation value TCSB with the working temperature fluctuation threshold TPJy;
If the point initial temperature fluctuation value TCSB is smaller than or equal to the working temperature fluctuation threshold TPJy; generating a normal signal; then checking and detecting isothermal control work; executing step S04;
If the point initial temperature fluctuation value TCSB is larger than the working temperature fluctuation threshold TPJy; generating an anomaly signal; prompting abnormal temperature deviation of a reaction chamber in the PCR instrument, checking whether the aging or abnormal connection of the Peltier occurs, and avoiding the work such as temperature abnormality affecting detection or inspection in the process of temperature control inspection;
In the step, the initial states of the Peltier at a plurality of points are checked, so that the temperature deviation of the interior Peltier is avoided when the temperature of the interior Peltier is recovered to the room temperature after the continuous operation of the PCR instrument, and the reused measuring result of the PCR instrument is further influenced;
step S04: acquiring an initial temperature value TFS and a target temperature value TBM of a reaction chamber in a PCR instrument;
comparing the initial temperature TFS of the reaction chamber with a target temperature TBM;
If the initial temperature TFS of the reaction chamber is smaller than the target temperature TBM, generating a temperature rise signal; namely, the reaction chamber of the PCR instrument needs to be heated to realize the temperature heating control of the reaction chamber; executing step S05;
If the initial temperature value TFS of the reaction chamber is equal to the target temperature value TBM, generating a temperature maintaining signal; step S07 is performed; when the target temperature of the PCR instrument is the same as the initial temperature, the constant temperature control is only needed for the reaction chamber, so that the reaction, detection and other inaccuracy in the reaction chamber caused by the larger temperature fluctuation influence of the reaction chamber are avoided;
if the initial temperature TFS of the reaction chamber is greater than the target temperature TBM, generating a temperature reduction signal; executing step S06; at this time, the temperature of the reaction chamber in the PCR instrument needs to be reduced;
Step S05: based on the temperature rising signal, controlling the current direction of the peltier to enable the peltier to heat the reaction chamber; heating the reaction chamber of the PCR instrument; until the reaction temperature rises to a target temperature value TBM;
The temperature state change of the peltier device in the temperature rising process can be monitored abnormally based on the state, and the temperature state change is specifically as follows:
Step S051: dividing time nodes in heating time by taking unit time t as interval duration, and marking the time nodes as j, wherein j is 0, 1, 2 and 3 … …; when j is 0, TFZ j is the initial temperature value TFS of the reaction chamber;
Step S052: the temperature value of the reaction chamber at the moment of each time node j in the heating time is obtained and marked as TFZ j,
Calculating and obtaining a temperature change rate value TBV j of the reaction chamber at a time node j in heating time; wherein,；
Step S053: acquiring a point location temperature value of each Peltier at the moment of each time node j in heating time, and marking the point location temperature value as TCS （i,j）;
Calculating and obtaining the point temperature change rate of the Peltier at the moment of the time node j in the heating time, and marking the point temperature change rate as TDV （i,j）; wherein, ；
Step S054; summing and averaging the point position temperature change rates of the Peltier of the i point positions in the PCR instrument to obtain the point position temperature change average rate TDVJZ j of the Peltier at the moment of the time node j;
Step S055: by passing through Calculating a temperature conduction ratio TCD j of the Peltier at the moment of the time node j; when the temperature of the peltier increases at a certain rate, the temperature of the reaction chamber should increase at the same rate, so that the larger TCD j, the better the heat conduction state;
Judging whether the temperature conduction ratio TCD j of the Peltier at the moment of the time node j meets a temperature conduction threshold TCDy or not;
if the temperature conduction ratio TCD j is greater than or equal to the temperature conduction threshold TCDy; generating a Peltier heat conduction stable signal;
If the temperature conduction ratio TCD j is less than the temperature conduction threshold TCDy; generating a Peltier heat conduction abnormal signal; the abnormal heat conduction signal is fed back to a worker to remind the worker that the temperature conduction of the PCR instrument is abnormal, the heat conduction is affected due to the ageing condition of the Peltier, and the PCR instrument is overhauled;
The temperature of the reaction chamber is transferred through the Peltier to further realize the purpose of temperature rise, so when the temperature of the Peltier rises at a certain speed, the temperature of the reaction chamber should rise at the same speed, therefore, the larger the temperature conduction ratio is, the better the heat conduction state is, if the temperature conduction ratio is lower, the temperature of the reaction chamber rises slowly, the temperature of the Peltier rises faster, namely, the heat in the Peltier cannot realize better heat transfer, and at the moment, the abnormal heat conduction performance of the Peltier is shown, and abnormal working or aging conditions exist; at this time, abnormal prompt can be directly carried out, and the work is stopped, so that the problem that the stability of the PCR instrument is influenced by the fact that the temperature in the Peltier is too high or the normal work of a circuit is influenced after the temperature of the reaction chamber reaches a target temperature value is avoided; the potential safety hazard is avoided;
Step S056: acquiring heating current values of Peltier at the moment of each time node j in heating time and marking the heating current values as IR j;
Carrying out standard deviation calculation on a heating current value IR j in heating time to obtain a heating current fluctuation value IRB;
Meanwhile, standard deviation calculation is carried out on point location temperature change average rate TDVJZ j of the Peltier in heating time, so as to obtain a point location temperature change rate fluctuation value TDVJZB;
step S057: by passing through Calculating to obtain a current heating conversion deviation value IZT;
Comparing the current heating conversion deviation value IZT with a current heating conversion deviation threshold IZTy;
if the current heating conversion deviation value IZT is less than or equal to the current heating conversion deviation threshold IZTy; generating a Peltier heating conversion normal signal;
If the current heating conversion deviation value IZT is greater than the current heating conversion deviation threshold IZTy; generating a Peltier heating conversion abnormal signal.
Because the heating effect of the peltier is affected by the current, when the current fluctuates, the temperature change rate of the peltier also fluctuates, therefore, when the temperature of the reaction chamber reaches a target temperature value in the heating process, the standard deviation calculation is performed on the current value of each time node in the heating process to judge the stability of the current in the heating process, meanwhile, the standard deviation calculation is performed on the point temperature change average rate of each time node to judge the temperature rise rate stability of the peltier in the heating process, when the current is close to the temperature rise rate fluctuation of the peltier, the temperature change rate fluctuation of the peltier is accurate, at the moment, the peltier heating stability is indicated, when the difference between the current and the temperature rise rate fluctuation of the peltier is larger, namely, when the current heating conversion deviation value is larger, the peltier heating instability is indicated, in the whole heating process, the peltier has an abnormal working state, at the moment, the peltier needs to be checked, so that the problem of inaccuracy in the temperature control process is avoided;
step S06: based on the temperature reduction signal, controlling the current direction of the peltier to enable the peltier to cool the reaction chamber and cool the reaction chamber of the PCR instrument; until the temperature of the reaction chamber is reduced to a target temperature value TBM;
the temperature state change of the peltier device in the cooling process can be monitored abnormally based on the state of the peltier device, and the temperature state change is specifically as follows:
step S061: dividing time nodes in the cooling time by taking unit time t as interval duration, and marking the time nodes as k, wherein k is 0, 1, 2 and 3 … …; when k is 0, TXZ k is the initial temperature value TFS of the reaction chamber;
Step S062: obtaining the temperature value of the reaction chamber at the moment k of each time node in the cooling time, marking the temperature value as TXZ k,
Calculating and obtaining a reaction chamber temperature change rate value TXV k at a time node k in the cooling time; wherein,；
Step S063: acquiring a point location temperature value of each Peltier at the moment of each time node k in cooling time, and marking the point location temperature value as TXS （i,k）;
Calculating and obtaining the point temperature change rate of the Peltier at the moment of the time node k in the cooling time, and marking the point temperature change rate as TXDV （i,k）; wherein, ；
Step S064; summing and averaging the point position temperature change rates of the Peltier of the i point positions in the PCR instrument to obtain the point position temperature change average rate TDVXZ k of the Peltier at the moment of the time node k;
Step S065: by passing through Calculating a cooling conduction ratio TXD k of the Peltier at the moment k of the time node;
Judging whether the cooling conduction ratio TXD k of the Peltier at the moment of the time node k meets a cooling conduction threshold TXDy or not;
if the cooling conduction ratio TXD k is greater than or equal to the cooling conduction threshold TXDy; generating a Peltier cooling stable signal;
If the cooling conduction ratio TXD k is less than the cooling conduction threshold TXDy; generating a Peltier cooling abnormal signal; feeding back a cooling abnormal signal to a worker to remind the worker that the temperature conduction of the PCR instrument is abnormal, the temperature of the PCR instrument is influenced by the aging condition of the Peltier, and overhauling the PCR instrument;
The temperature of the reaction chamber is transferred through the Peltier to realize the purpose of temperature reduction, so when the temperature of the Peltier is reduced at a certain speed, the temperature of the reaction chamber is reduced at the same speed, and therefore, the larger the temperature conduction ratio is, the better the cooling state is, if the temperature conduction ratio is lower, the slower the temperature of the reaction chamber is reduced, the faster the temperature of the Peltier is reduced, namely, the better cooling effect cannot be realized in the Peltier, and at the moment, the abnormal working or aging condition exists in the refrigeration performance of the Peltier; at this time, the abnormal prompt can be directly carried out, and the work is stopped, so that the abnormal working condition of the internal element is avoided;
Step S066: acquiring a cooling current value of the Peltier at the moment k of each time node in cooling time and marking the cooling current value as IL k;
standard deviation calculation is carried out on the cooling current value IL k in the cooling time to obtain a cooling current fluctuation value ILB;
meanwhile, standard deviation calculation is carried out on the point location temperature change average rate TDVXZ k of the Peltier in the cooling time to obtain a point location temperature change rate fluctuation value TDVXZB;
Step S067: by passing through Calculating to obtain a current refrigeration conversion deviation value ILT;
comparing the current refrigeration conversion deviation value ILT with a current refrigeration conversion deviation threshold ILTy;
If the current refrigeration conversion deviation value ILT is smaller than or equal to the current refrigeration conversion deviation threshold ILTy; generating a Peltier refrigeration conversion normal signal;
If the current refrigeration conversion deviation value ILT is greater than or equal to the current refrigeration conversion deviation threshold ILTy; generating a Peltier refrigeration conversion abnormal signal;
Because the refrigerating effect of the peltier is also affected by the current, when the current fluctuates, the temperature change rate of the peltier refrigerating process also fluctuates, therefore, when the temperature of the reaction chamber reaches a target temperature value in the refrigerating process, the standard deviation calculation is performed on the current value of each time node in the refrigerating process to judge the stability of the current in the refrigerating process, meanwhile, the standard deviation calculation is performed on the point position temperature change average rate of each time node to judge the temperature reduction rate stability of the peltier in the refrigerating process, when the current is close to the temperature reduction rate fluctuation of the peltier, the peltier refrigerating effect is accurately indicated to be subject to the fluctuation of the current, at the moment, the peltier refrigerating is stable, when the difference between the current and the peltier temperature reduction rate fluctuation is large, namely, the current refrigerating conversion deviation value is large, the peltier refrigerating is unstable, and in the whole refrigerating process, the peltier has an abnormal working state, at the moment, the peltier is required to be checked, so that the problem of inaccuracy in the temperature control process is avoided;
Step S07: based on the temperature maintaining signal, opening the Peltier to be in a standby state, monitoring, maintaining and regulating the temperature value of a reaction chamber in the PCR instrument by taking the unit time t as the interval duration, and then executing the step S04; in the process of maintaining the temperature of the reaction chamber, when the temperature of the reaction chamber is reduced or increased, the temperature increase or the temperature reduction regulation step can be carried out, so that the accurate control of the temperature is better realized.
In the steps, the temperature in the reaction chamber is controlled through the multi-point peltier, the response speed is high, meanwhile, the peltier state in the initial state is checked, and the phenomenon that the temperature deviation occurs when the temperature of the inner peltier returns to the room temperature after the continuous operation of the PCR instrument is avoided, so that the measurement result of the reuse of the PCR instrument is affected;
In addition, in the working process, through the real-time monitoring of the temperature of the reaction chamber and the temperature of the reaction chamber in the temperature rising process and the temperature reducing process, whether the working state of the peltier is abnormal or not can be timely found in the temperature regulating process, the working stability and safety of the PCR instrument are ensured, meanwhile, after the temperature regulating is finished, the influence of the current change in the temperature regulating process on the working state of the peltier can be analyzed, and further the working state of the peltier in the working process is judged to be changed according to the current change, so that whether the temperature regulating of the peltier is accurate or not can be found, the abnormal condition of the peltier can be timely found, and the problem of the working of the PCR instrument is avoided.
Example III
Based on the above embodiments, the present embodiment provides a temperature control method of a PCR instrument, including that when the temperature of a reaction chamber in the PCR instrument needs to continuously achieve a "raise-hold-lower" state, the working state of the PCR instrument is to execute step S01-step S03 and obtain a normal signal; then executing step S04-step S05; after reaching the target temperature value, step S07 is performed; after being maintained for a certain period of time, step S06 is performed;
in the present embodiment, not limited to only the example explanation of "raise-hold-lower";
Similarly, in the process of maintaining the temperature of the reaction chamber, when the temperature of the reaction chamber is reduced or increased, the temperature increase or the temperature reduction regulation step can be performed, so that the temperature can be accurately controlled better, and the working temperature accuracy of the PCR instrument is ensured.
The foregoing describes one embodiment of the present invention in detail, but the description is only a preferred embodiment of the present invention and should not be construed as limiting the scope of the invention. All equivalent changes and modifications within the scope of the present invention are intended to be covered by the present invention.
Claims (8)
1. A temperature control system for a PCR instrument, comprising:
And a data acquisition module: the method comprises the steps of obtaining the point positions of the Peltier in a reaction chamber in a PCR instrument, and marking the point positions of the Peltier; acquiring a point location initial temperature value of a Peltier of a reaction chamber in a PCR instrument;
And a data processing module: the method comprises the steps of (1) carrying out standard deviation calculation on a point position initial temperature value TCS i of the peltier in a reaction chamber in a PCR instrument to obtain a point position initial temperature fluctuation value TCSB of the peltier in the reaction chamber;
and a state verification module: the method comprises the steps of acquiring a reaction chamber working temperature fluctuation threshold TPJy of a PCR instrument; comparing the initial temperature fluctuation value TCSB of the point position with a working temperature fluctuation threshold TPJy; and obtaining a normal signal or an abnormal signal;
the work judging module is used for: the method comprises the steps of obtaining an initial temperature value TFS and a target temperature value TBM of a reaction chamber in a PCR instrument; comparing the initial temperature TFS of the reaction chamber with a target temperature TBM; and generating a temperature execution signal, wherein the temperature execution signal comprises: a temperature increase signal, a temperature hold signal, and a temperature decrease signal;
the execution module: controlling the working mode of the Peltier based on the temperature execution signal; regulating and controlling the temperature of a reaction chamber of the PCR instrument; comprises a state monitoring unit and a regulation analysis unit;
The state monitoring unit is used for carrying out abnormal monitoring on the working state in the temperature regulation and control process and obtaining a state signal; wherein the status signal comprises: a heat conduction stabilizing signal, a heat conduction abnormal signal, a cooling stabilizing signal and a cooling abnormal signal;
the state monitoring unit is used for monitoring the working state in the temperature regulation process abnormally, and comprises the following steps:
Dividing time nodes in heating time by taking unit time t as interval duration, and marking the time nodes as j, wherein j is 0, 1, 2 and 3 … …;
The temperature value of the reaction chamber at the moment of each time node j in the heating time is obtained and marked as TFZ j,
Calculating and obtaining a temperature change rate value TBV j of the reaction chamber at a time node j in heating time;
；
acquiring a point location temperature value of each Peltier at the moment of each time node j in heating time, and marking the point location temperature value as TCS （i,j）;
calculating and obtaining the point temperature change rate of the Peltier at the moment of the time node j in the heating time, and marking the point temperature change rate as TDV （i,j）;
；
summing and averaging the point position temperature change rates of the Peltier of the i point positions in the PCR instrument to obtain the point position temperature change average rate TDVJZ j of the Peltier at the moment of the time node j;
By passing through Calculating a temperature conduction ratio TCD j of the Peltier at the moment of the time node j;
if the temperature conduction ratio TCD j is greater than or equal to the temperature conduction threshold TCDy; generating a Peltier heat conduction stable signal;
If the temperature conduction ratio TCD j is less than the temperature conduction threshold TCDy; generating a Peltier heat conduction abnormal signal.
2. The temperature control system of a PCR instrument according to claim 1, wherein the regulation analysis unit is configured to analyze the regulation data and obtain a conversion signal; wherein converting the signal comprises: a heating conversion normal signal, a heating conversion abnormal signal, a cooling conversion normal signal, and a cooling conversion abnormal signal.
3. The temperature control system of a PCR instrument according to claim 1, wherein the status verification module: comparing the point initial temperature fluctuation value TCSB with the working temperature fluctuation threshold TPJy;
If the point initial temperature fluctuation value TCSB is smaller than or equal to the working temperature fluctuation threshold TPJy; generating a normal signal;
If the point initial temperature fluctuation value TCSB is larger than the working temperature fluctuation threshold TPJy; an exception signal is generated.
4. The temperature control system of a PCR instrument according to claim 1, wherein the operation determination module compares the initial temperature TFS of the reaction chamber with the target temperature TBM;
If the initial temperature TFS of the reaction chamber is smaller than the target temperature TBM, generating a temperature rise signal;
If the initial temperature value TFS of the reaction chamber is equal to the target temperature value TBM, generating a temperature maintaining signal;
if the initial temperature TFS of the reaction chamber is greater than the target temperature TBM, a temperature reduction signal is generated.
5. The temperature control system of claim 1, wherein the execution module regulates a temperature of a reaction chamber of the PCR instrument based on the temperature execution signal, comprising:
Based on the temperature rising signal, controlling the current direction of the peltier to enable the peltier to heat the reaction chamber; heating the reaction chamber of the PCR instrument;
based on the temperature reduction signal, controlling the current direction of the peltier to enable the peltier to be in a refrigeration mode for the reaction chamber; cooling the reaction chamber of the PCR instrument;
Based on the temperature maintaining signal, the Peltier is started to be in a standby state, and the temperature value of a reaction chamber in the PCR instrument is monitored by taking the unit time t as the interval duration.
6. The temperature control system of a PCR instrument according to claim 1, wherein the state monitoring unit performs abnormality monitoring on the operation state in the temperature regulation process, and includes:
dividing time nodes in the cooling time by taking unit time t as interval duration, and marking the time nodes as k, wherein k is 0, 1, 2 and 3 … …;
Obtaining the temperature value of the reaction chamber at the moment k of each time node in the cooling time, marking the temperature value as TXZ k,
Calculating and obtaining a reaction chamber temperature change rate value TXV k at a time node k in the cooling time;
；
Acquiring a point location temperature value of each Peltier at the moment of each time node k in cooling time, and marking the point location temperature value as TXS （i,k）;
calculating and obtaining the point temperature change rate of the Peltier at the moment of the time node k in the cooling time, and marking the point temperature change rate as TXDV （i,k）;
；
Summing and averaging the point position temperature change rates of the Peltier of the i point positions in the PCR instrument to obtain the point position temperature change average rate TDVXZ k of the Peltier at the moment of the time node k;
By passing through Calculating a cooling conduction ratio TXD k of the Peltier at the moment k of the time node;
if the cooling conduction ratio TXD k is greater than or equal to the cooling conduction threshold TXDy; generating a Peltier cooling stable signal;
If the cooling conduction ratio TXD k is less than the cooling conduction threshold TXDy; generating a Peltier cooling abnormal signal.
7. The temperature control system of a PCR instrument according to claim 1, wherein the operation mode of the control analysis unit includes:
Acquiring heating current values of Peltier at the moment of each time node j in heating time and marking the heating current values as IR j;
Carrying out standard deviation calculation on a heating current value IR j in heating time to obtain a heating current fluctuation value IRB;
Meanwhile, standard deviation calculation is carried out on point location temperature change average rate TDVJZ j of the Peltier in heating time, so as to obtain a point location temperature change rate fluctuation value TDVJZB;
By passing through Calculating to obtain a current heating conversion deviation value IZT;
Comparing the current heating conversion deviation value IZT with a current heating conversion deviation threshold IZTy;
if the current heating conversion deviation value IZT is less than or equal to the current heating conversion deviation threshold IZTy; generating a Peltier heating conversion normal signal;
If the current heating conversion deviation value IZT is greater than the current heating conversion deviation threshold IZTy; generating a Peltier heating conversion abnormal signal.
8. The temperature control system of a PCR instrument according to claim 6, wherein the operation mode of the control analysis unit includes:
Acquiring a cooling current value of the Peltier at the moment k of each time node in cooling time and marking the cooling current value as IL k;
standard deviation calculation is carried out on the cooling current value IL k in the cooling time to obtain a cooling current fluctuation value ILB;
meanwhile, standard deviation calculation is carried out on the point location temperature change average rate TDVXZ k of the Peltier in the cooling time to obtain a point location temperature change rate fluctuation value TDVXZB;
By passing through Calculating to obtain a current refrigeration conversion deviation value ILT;
comparing the current refrigeration conversion deviation value ILT with a current refrigeration conversion deviation threshold ILTy;
If the current refrigeration conversion deviation value ILT is smaller than or equal to the current refrigeration conversion deviation threshold ILTy; generating a Peltier refrigeration conversion normal signal;
if the current refrigeration conversion deviation value ILT is greater than or equal to the current refrigeration conversion deviation threshold ILTy; generating a Peltier refrigeration conversion abnormal signal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410599370.5A CN118170190B (en) | 2024-05-15 | 2024-05-15 | Temperature control system of PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410599370.5A CN118170190B (en) | 2024-05-15 | 2024-05-15 | Temperature control system of PCR instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN118170190A CN118170190A (en) | 2024-06-11 |
| CN118170190B true CN118170190B (en) | 2024-08-02 |
Family
ID=91357089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410599370.5A Active CN118170190B (en) | 2024-05-15 | 2024-05-15 | Temperature control system of PCR instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118170190B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN215856127U (en) * | 2021-08-19 | 2022-02-18 | 北京卓诚惠生生物科技股份有限公司 | Thermal cycle structure of PCR amplification platform |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210721166U (en) * | 2019-11-25 | 2020-06-09 | 无锡芯谱半导体科技有限公司 | Temperature control device for semiconductor production |
| CN112903084B (en) * | 2021-01-22 | 2022-07-19 | 中化学交通建设集团有限公司 | Optical fiber vibration sensing optical path fault diagnosis method and related equipment |
| CN114294856B (en) * | 2021-12-13 | 2023-08-25 | 迈克医疗电子有限公司 | Method, device, medium, equipment and instrument for improving Peltier refrigeration efficiency |
| CN114647186B (en) * | 2022-03-23 | 2023-08-01 | 无锡百泰克生物技术有限公司 | Control method and control device for rapid constant temperature of PCR detector and PCR detector |
| CN114798316B (en) * | 2022-06-27 | 2022-09-13 | 常州铭赛机器人科技股份有限公司 | Control method for keeping constant temperature of glue solution |
| CN117781506A (en) * | 2023-12-26 | 2024-03-29 | 珠海丽珠试剂股份有限公司 | Control method and device of Peltier refrigerating system and electronic equipment |
| CN117503204B (en) * | 2024-01-04 | 2024-03-22 | 北京超数时代科技有限公司 | Ultrasonic AI digital diagnosis and treatment system |
-
2024
- 2024-05-15 CN CN202410599370.5A patent/CN118170190B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN215856127U (en) * | 2021-08-19 | 2022-02-18 | 北京卓诚惠生生物科技股份有限公司 | Thermal cycle structure of PCR amplification platform |
Also Published As
| Publication number | Publication date |
|---|---|
| CN118170190A (en) | 2024-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109556943B (en) | Temperature correction method and device for constant temperature system, analysis instrument, and storage medium | |
| US8271230B2 (en) | System and methods for monitoring a thermoelectric heating and cooling device | |
| EP2556173B1 (en) | Improved thermal uniformity for thermal cycler instrumentation using dynamic control | |
| CN107949631B (en) | Temperature adjusting device | |
| KR20160039529A (en) | Method for amending amplification profile curve, method for obtaining information for sample analysis, recording medium and computer therefor | |
| CN118170190B (en) | Temperature control system of PCR instrument | |
| CN107762936A (en) | Temperature control equipment and its method | |
| CN116796209B (en) | Data processing method for monitoring storage environment temperature of detection kit | |
| WO2018150648A1 (en) | Temperature regulating device and nucleic-acid amplification device | |
| CN103210094A (en) | Genetic testing method and testing apparatus | |
| KR101251177B1 (en) | Melting profile analysis method and melting profile analysis device | |
| CN109609606B (en) | Method for determining relative enzyme activity of DNA polymerase | |
| CN114512416A (en) | Probe abnormality recognition method and device, storage medium and electronic device | |
| US20180015476A1 (en) | Thermal cycler for biological material reaction, biological material analysis apparatus, system for controlling rate of temperature change in biological material reaction, method for controlling rate of temperature change in biological material reaction, biological material analysis method, and temperature control program for biological material reaction | |
| CN114646477B (en) | Test method and system for automobile hybrid power system | |
| Frahm et al. | Leveling response factors in the electrospray ionization process using a heated capillary interface | |
| CN117649879B (en) | Temperature compensation method, system and medium for digital PCR of medical equipment | |
| EP3126518B1 (en) | Methods and systems for pcr quantitation | |
| EP2233587A1 (en) | Nucleic-acid amplification judging method and nucleic-acid amplification judging device | |
| JP7493300B2 (en) | Analysis program and analysis device | |
| CN117555371A (en) | PCR temperature control method, system and medium based on medical equipment | |
| CN110863102B (en) | Method and device for correcting thermocouple value of heating section of annealing furnace | |
| CN111831030B (en) | Thermal control device and automatic thermal control adjusting method | |
| CN114995555A (en) | Reaction vessel temperature control method, storage medium and sample detection device | |
| CN217467541U (en) | High-precision temperature control system used in surface plasma resonance reaction bin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |