CN214991514U

CN214991514U - Nucleic acid extraction instrument

Info

Publication number: CN214991514U
Application number: CN202022499829.4U
Authority: CN
Inventors: 肖杰; 王丽玲; 胡荣; 余义文; 冯介明; 宋家武
Original assignee: Zhuhai Sinochips Biotechnology Co ltd
Current assignee: Zhuhai Sinochips Biotechnology Co ltd
Priority date: 2020-11-02
Filing date: 2020-11-02
Publication date: 2021-12-03
Anticipated expiration: 2030-11-02

Abstract

The utility model discloses a nucleic acid extraction appearance, include: the test device comprises a machine body, wherein an experiment cabin is arranged in the machine body, a positioning seat is arranged in the experiment cabin, and a deep hole plate is fixed on the positioning seat; the extraction assembly is arranged in the experiment cabin and is positioned above the deep hole plate; the induced air assembly comprises an air inlet mechanism and an air outlet mechanism which are respectively arranged on the machine body, and the joints of the air inlet mechanism and the air outlet mechanism with the machine body are respectively provided with an air inlet gate and an air outlet gate. The utility model utilizes the air inlet gate and the air outlet gate to control the air exchange between the experiment chamber and the external environment, so that the extraction operation can be carried out under the closed environment and the air exchange condition, and the heat generated during stirring and extraction is taken away by utilizing the air flow; and the cell activity is kept by utilizing the air communication of the environment or under the closed condition, so that the operation is convenient.

Description

Nucleic acid extraction instrument

Technical Field

The utility model relates to a nucleic acid extraction technical field, in particular to nucleic acid extraction appearance.

Background

The nucleic acid extractor is an instrument which automatically finishes the extraction of sample nucleic acid by using a matched nucleic acid extraction reagent. The method is widely applied to various fields of disease control centers, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like.

At present, the nucleic acid extraction instrument on the market aims at reagent extraction, the temperature and the gas flow in the instrument cannot be controlled, cells can effectively survive for a period of time at a proper temperature and fresh air before extraction, and the temperature in the instrument is overheated due to a large amount of heat generated in an experimental cabin in the extraction process, so that the extraction efficiency and the service life of the instrument are influenced.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at solving one of the technical problem that exists among the prior art at least. Therefore, the utility model provides a nucleic acid extraction appearance can keep the cell activity in a certain time, improves extraction efficiency, avoids influencing and draws the quality.

According to the utility model discloses a nucleic acid extraction appearance of first aspect embodiment includes: the test device comprises a machine body, wherein an experiment cabin is arranged in the machine body, a positioning seat is arranged in the experiment cabin, and a deep hole plate is fixed on the positioning seat; the extraction assembly is arranged in the experiment cabin and is positioned above the deep hole plate; the induced air assembly comprises an air inlet mechanism and an air outlet mechanism which are respectively arranged on the machine body, and the joints of the air inlet mechanism and the air outlet mechanism with the machine body are respectively provided with an air inlet gate and an air outlet gate.

According to the utility model discloses nucleic acid extraction appearance has following beneficial effect at least: the air inlet gate and the air outlet gate are used for controlling the air communication between the experiment chamber and the external environment, so that the extraction operation can be carried out under a closed environment and an air communication condition, and the heat generated during stirring and extraction is taken away by airflow; and the cell activity is kept by utilizing the air communication of the environment or under the closed condition, so that the operation is convenient.

According to some embodiments of the utility model, be equipped with a plurality of reference columns on the positioning seat, the bottom of deep hole board be equipped with reference column matched with locating hole.

According to some embodiments of the utility model, the positioning seat both sides opposition is equipped with two clamp splice slide rails, two be equipped with the tight piece of first clamp and the tight piece of second clamp on the clamp splice slide rail respectively, be equipped with first clamping piece on the tight piece of first clamp, be equipped with second positioning clamping piece on the tight piece of second clamp, still be equipped with reset spring on the clamp splice slide rail, reset spring drive first clamp the tight piece with the tight piece of second clamp is pressed from both sides tightly the positioning seat both sides.

According to the utility model discloses a some embodiments, the end of clamp splice slide rail is equipped with the spring holder, reset spring's one end is connected on the spring holder, and the other end butt is in on the tight piece of first clamp splice/second clamp splice.

According to the utility model discloses a some embodiments, be equipped with air intake and air outlet on the organism, air inlet mechanism sets up on the air intake, air-out mechanism sets up on the air outlet, the air intake with the air outlet sets up respectively the both sides in experiment cabin.

According to some embodiments of the utility model, the air inlet mechanism includes air intake fan and sets up the filter screen and the filter plate in the air intake fan outside, the filter screen sets up the air inlet side of filter plate.

According to some embodiments of the utility model, the extraction component includes support frame, magnetic rod and magnetic stirring cover, the magnetic stirring cover is established the magnetic rod is outer.

According to some embodiments of the utility model, be equipped with heating element in the experiment cabin, heating element includes heating pipe and temperature sensor.

According to the utility model discloses a some embodiments, all be equipped with the heat insulating board on air inlet gate and the air-out gate.

According to some embodiments of the utility model, the experiment cabin outside is equipped with transparent window cover, transparent window cover with the junction in experiment cabin is equipped with sealed the pad.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a schematic diagram of the internal structure of a nucleic acid extractor according to an embodiment of the present invention;

FIG. 2 is a front view of the nucleic acid extractor shown in FIG. 1;

FIG. 3 is a schematic view showing the structure of the nucleic acid extracting apparatus of FIG. 1, with the transparent cover omitted;

fig. 4 is a top view of the positioning socket shown in fig. 1.

The attached drawings are marked as follows:

the device comprises a machine body 100, an experiment cabin 110, a positioning seat 120, a positioning column 121, a deep hole plate 130, a clamping block sliding rail 140, a first clamping block 150, a first positioning clamping piece 151, a second clamping block 160, a second positioning clamping piece 161, a return spring 170, a spring seat 180, a transparent window cover 190 and a sealing gasket 191;

an extraction component 200;

the air inlet mechanism 310, the filter screen 311, the filter plate 312, the air inlet fan 313, the air outlet mechanism 320, the air outlet fan 321, the anti-filter screen 322, the air inlet gate 330, the air outlet gate 340, the air inlet 350 and the air outlet 360;

heating the tube 410, manipulating the touch screen 420.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated with respect to the orientation description, such as up, down, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, a plurality of means are one or more, a plurality of means are two or more, and the terms greater than, less than, exceeding, etc. are understood as not including the present number, and the terms greater than, less than, within, etc. are understood as including the present number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless there is an explicit limitation, the words such as setting, installation, connection, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in combination with the specific contents of the technical solution.

The following describes a nucleic acid isolation instrument according to an embodiment of the present invention with reference to FIGS. 1 to 4.

As shown in fig. 1 to 4, a nucleic acid extracting apparatus comprises a body 100, an extracting assembly 200 and an induced draft assembly, wherein the body 100 is provided with an experimental chamber 110, the experimental chamber 110 is a site for extracting nucleic acid, i.e., DNA/RNA, the experimental chamber 110 is provided with a positioning seat 120, the positioning seat 120 is fixed with a deep hole plate 130, the deep hole plate 130 is detachably mounted on the positioning seat 120, the deep hole plate 130 is provided with a plurality of containing holes for containing a carrier plate of cellular fluid, test tubes or other open containers containing the cellular fluid are placed on the containing holes, the extracting assembly 200 is arranged in the experimental chamber 110 and located above the deep hole plate 130, the cellular fluid on the deep hole plate 130 is smashed and absorbs broken nucleic acid, and the nucleic acid is placed in a new container, thereby completing the extracting process. The induced air assembly comprises an air inlet mechanism 310 and an air outlet mechanism 320 which are respectively arranged on the machine body 100, an air inlet gate 330 is arranged at the joint of the air inlet mechanism 310 and the machine body 100, an air outlet gate 340 is arranged at the joint of the air outlet mechanism 320 and the machine body 100, and the air inlet gate 330 and the air outlet gate 340 are used for controlling the communication between the experiment cabin 110 and the outside air to form a closed cabin or an open induced air cabin. The induced air assembly is used for passing clean air into the experiment chamber 110 and taking away heat generated during mashing, so that the extraction process is prevented from being influenced due to excessive temperature rise of the heat.

In some embodiments of the present invention, the positioning seat 120 is provided with a plurality of positioning pillars 121, the positioning pillars 121 are at least three, and are disposed on the positioning seat 120 in multiple sides, and the bottom of the deep hole plate 130 is provided with and positioned to the matched positioning hole. When the deep hole plate 130 is installed on the positioning base 120, the positioning hole is sleeved with the positioning column 121, so that the positioning is accurate, and the extracting assembly 200 can accurately smash and extract nucleic acid conveniently.

The utility model discloses an in some embodiments, positioning seat 120 both sides are opposite and are equipped with two clamp splice slide rails 140, be equipped with first clamp splice 150 and the tight piece 160 of second clamp on two clamp splice slide rails 140 respectively, first clamp splice 150 and the tight piece 160 of second clamp can be along clamp splice slide rail 140 lateral shifting, be equipped with first positioning clamping piece 151 on the first clamp splice 150, be equipped with second positioning clamping piece 161 on the tight piece 160 of second clamp, still be equipped with reset spring 170 on the clamp splice slide rail 140, reset spring 170 drives first clamp splice 150 and the tight piece 160 of second clamp and cliies positioning seat 120 both sides, reset spring 170 provides the clamp force of two clamp splices, fix a position the tight installation on reset spring 170 with deep hole board 130, and convenient for installation.

Specifically, the spring seat 180 is disposed at the end of the clamping block slide rail 140, the spring seat 180 is a power storage base of the return spring 170, one end of the return spring 170 is connected to the spring seat 180, and the other end of the return spring 170 abuts against the first clamping block 150/the second clamping block 160 to provide a spring force to drive the first clamping block 150 and the second clamping block 160 to clamp towards the positioning seat 120.

In some embodiments of the present invention, the machine body 100 is provided with an air inlet 350 and an air outlet 360, the air inlet mechanism 310 is disposed on the air inlet 350, the air outlet mechanism 320 is disposed on the air outlet 360, the air current enters the experiment chamber 110 from the air inlet 350, and flows out from the air outlet 360, the air inlet 350 and the air outlet 360 are disposed on two sides of the experiment chamber 110 respectively, the wind power enters the experiment chamber 110 from the air inlet 350 through the air inlet mechanism 310, and then is discharged through the air outlet 360, so that the experiment chamber 110 forms the air current, and the heat generated during extraction is taken away.

Specifically, the air intake mechanism 310 comprises an air intake fan 313, and a filter screen 311 and a filter plate 312 which are arranged outside the air intake fan 313, wherein the filter screen 311 is arranged on the air intake side of the filter plate 312, and when air enters the experiment chamber 110 from the air intake 350, the air passes through the filter screen 311 and the filter plate 312 in sequence, so that the air is filtered and cleaned, and the cell sap is prevented from being polluted by bacteria. The air outlet mechanism 320 comprises an air outlet fan 321 and a filter screen 322, wherein the filter screen 322 is used for preventing external bacteria from entering the experiment chamber 110.

In some embodiments of the utility model, extract subassembly 200 includes support frame, bar magnet and magnetic stirring cover, and the magnetic stirring cover is established at the bar magnet skin, and the support frame is the crane, stretches into bar magnet and magnetic stirring cover to the cell sap, and the rotation of magnetic stirring cover stirs the cell sap and makes the cell breakage, and the bar magnet absorbs nucleic acid, puts into the storage tube with the nucleic acid that absorbs afterwards, accomplishes and draws the process. The support frame can carry out elevating movement, still can carry out lateral motion, is equipped with driving piece and motion module in the organism 100, and the driving piece passes through motion module drive support frame, makes bar magnet and magnetic stirring cover move in experiment cabin 110. In addition, the extraction assembly 200 can also be a commercially available or other existing nucleic acid extraction mechanism.

In some embodiments of the present invention, a heating module is disposed in the experiment chamber 110, the heating module includes a heating tube 410 and a temperature sensor, the heating tube 410 and the temperature sensor are used to control the internal temperature of the experiment chamber 110, so that the cell sap or the nucleic acid is in a suitable temperature condition. Wherein, the heating tube 410 may be an electrical heating tube.

The utility model discloses an in some embodiments, all be equipped with the heat insulating board on air inlet gate 330 and the air outlet gate 340, the experiment cabin 110 outside is equipped with transparent window cover 190, and the heat insulating board is used for keeping the inside temperature of experiment cabin 110, reduces the heat and exchanges, and transparent window cover 190 then is used for observing the inside condition of experiment cabin 110. The joint of the transparent window cover 190 and the experiment chamber 110 is provided with a gasket 191, and the gasket 191 improves the sealing effect of the experiment chamber 110.

In some embodiments of the present invention, the machine body 100 is provided with a control touch screen 420, and the control touch screen 420 is connected to the internal controller of the machine body 100 for controlling the operation of each internal component.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.

Claims

1. A nucleic acid extractor, comprising:

the testing device comprises a machine body (100), wherein an experiment cabin (110) is arranged in the machine body (100), a positioning seat (120) is arranged in the experiment cabin (110), and a deep hole plate (130) is fixed on the positioning seat (120);

an extraction assembly (200) disposed within the experimental chamber (110) and above the deep well plate (130);

the induced air assembly comprises an air inlet mechanism (310) and an air outlet mechanism (320) which are respectively arranged on the machine body (100), wherein an air inlet gate (330) and an air outlet gate (340) are respectively arranged at the connection part of the air inlet mechanism (310) and the air outlet mechanism (320) and the machine body (100).

2. The nucleic acid extractor according to claim 1, wherein the positioning base (120) is provided with a plurality of positioning posts (121), and the bottom of the deep hole plate (130) is provided with positioning holes matched with the positioning posts (121).

3. The nucleic acid extractor according to claim 2, wherein two clamping block slide rails (140) are oppositely arranged on two sides of the positioning seat (120), a first clamping block (150) and a second clamping block (160) are respectively arranged on the two clamping block slide rails (140), a first positioning clamping member (151) is arranged on the first clamping block (150), a second positioning clamping member (161) is arranged on the second clamping block (160), a return spring (170) is further arranged on the clamping block slide rails (140), and the return spring (170) drives the first clamping block (150) and the second clamping block (160) to be clamped on two sides of the positioning seat (120).

4. The nucleic acid extraction instrument according to claim 3, wherein a spring seat (180) is provided at the end of the clamp block slide rail (140), and one end of the return spring (170) is connected to the spring seat (180) and the other end abuts against the first clamp block (150)/the second clamp block (160).

5. The nucleic acid extractor of claim 1, wherein the body (100) is provided with an air inlet (350) and an air outlet (360), the air inlet mechanism (310) is disposed on the air inlet (350), the air outlet mechanism (320) is disposed on the air outlet (360), and the air inlet (350) and the air outlet (360) are disposed on two sides of the experiment chamber (110), respectively.

6. The nucleic acid extraction instrument according to claim 5, wherein the air intake mechanism (310) comprises an air intake fan (313) and a filter screen (311) and a filter plate (312) arranged outside the air intake fan (313), and the filter screen (311) is arranged on the air intake side of the filter plate (312).

7. The nucleic acid extractor of claim 1, wherein the extraction assembly (200) comprises a support frame, a magnetic rod and a magnetic stirring sleeve, and the magnetic stirring sleeve is sleeved on the outer layer of the magnetic rod.

8. The nucleic acid extractor of claim 1, wherein the experiment chamber (110) is provided with a heating assembly, and the heating assembly comprises a heating tube (410) and a temperature sensor.

9. The nucleic acid extractor of claim 1, wherein the air inlet shutter (330) and the air outlet shutter (340) are provided with heat insulation plates.

10. The nucleic acid extractor of claim 1, wherein a transparent window cover (190) is disposed outside the experiment chamber (110), and a sealing gasket (191) is disposed at the joint of the transparent window cover (190) and the experiment chamber (110).