CN220520480U - Temperature control module for 96-hole PCR (polymerase chain reaction) amplification instrument - Google Patents
Temperature control module for 96-hole PCR (polymerase chain reaction) amplification instrument Download PDFInfo
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- CN220520480U CN220520480U CN202321937719.9U CN202321937719U CN220520480U CN 220520480 U CN220520480 U CN 220520480U CN 202321937719 U CN202321937719 U CN 202321937719U CN 220520480 U CN220520480 U CN 220520480U
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- amplification instrument
- temperature
- instrument body
- control module
- placing
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- 230000003321 amplification Effects 0.000 title claims abstract description 41
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 41
- 238000003752 polymerase chain reaction Methods 0.000 title abstract description 10
- 238000010438 heat treatment Methods 0.000 claims abstract description 31
- 239000000523 sample Substances 0.000 claims abstract description 24
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 238000012544 monitoring process Methods 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims description 31
- 238000012408 PCR amplification Methods 0.000 claims description 21
- 230000005540 biological transmission Effects 0.000 claims description 10
- 230000017525 heat dissipation Effects 0.000 claims description 5
- 238000004891 communication Methods 0.000 claims description 4
- 125000006850 spacer group Chemical group 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 18
- 230000008859 change Effects 0.000 abstract description 7
- 230000004544 DNA amplification Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 108020004414 DNA Proteins 0.000 description 6
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000008236 heating water Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 238000007859 qualitative PCR Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000003303 reheating Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of temperature control modules, in particular to a temperature control module for a 96-hole PCR (polymerase chain reaction) amplification instrument, which comprises an amplification instrument body, wherein a controller is arranged on one side of the upper surface of the amplification instrument body, a groove is formed in the other side of the upper surface of the amplification instrument body, a sealing cover is movably inserted into the amplification instrument body through one side of the upper surface of the groove, a plurality of temperature monitoring probes are uniformly arranged on the lower surface of the sealing cover, a placing disc is arranged in the middle of the inner bottom surface of the groove of the upper surface of the amplification instrument body, a plurality of placing holes are uniformly formed in the upper surface of the placing disc, a heating cavity is formed in the lower surface of the placing disc, and a first heating rod is arranged on the inner surface of the heating cavity. The device has reasonable design, can realize the temperature adjustment of the sample tube by adopting a cold and hot water preheating exchange mode, is beneficial to accelerating the temperature change efficiency of the sample tube, and is convenient for maintaining the DNA amplification effect.
Description
Technical Field
The utility model relates to the technical field of temperature control modules, in particular to a temperature control module for a 96-hole PCR amplification instrument.
Background
The PCR amplification instrument is an instrument for amplifying specific DNA by Polymerase Chain Reaction (PCR), and is widely used for scientific research, clinical gene amplification, qualitative PCR gene amplification, fluorescent/enzyme endpoint-free quantitative DNA gene amplification, gene amplification of other gene analysis applications such as gene chips, and the like.
The PCR amplification instrument has the working principle that the purpose of gene amplification is realized by controlling the reaction temperature, and the primary amplification needs to be performed through 3 steps of high-temperature denaturation, low-temperature annealing and medium-temperature extension. Therefore, the temperature control module in the PCR amplification apparatus needs to perform cyclic adjustment of temperature change in a short time. However, the heat exchange efficiency of the temperature control module in the existing PCR amplification instrument is low, so that the temperature change process in the PCR amplification instrument is slow, and the amplification effect of DNA is easily affected. For this reason, there is a need to propose an improvement for solving the above-mentioned problems.
Disclosure of Invention
The utility model aims to solve the defects in the prior art, and provides a temperature control module for a 96-hole PCR (polymerase chain reaction) amplification instrument, which can realize temperature adjustment of a sample tube in a cold-hot water preheating exchange mode, is beneficial to accelerating the temperature change efficiency of the sample tube and is convenient for maintaining the amplification effect of DNA.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a 96 hole PCR amplification appearance is with control by temperature change module, includes the amplification appearance body, one side of amplification appearance body upper surface is equipped with the controller, the opposite side of amplification appearance body upper surface is seted up flutedly, the upper surface one side activity that the amplification appearance body passed through the recess is inserted and is equipped with sealed lid, the lower surface of sealed lid evenly is equipped with a plurality of temperature monitoring probes, the interior bottom surface middle part of amplification appearance body upper surface recess is equipped with places the dish, a plurality of holes of placing have evenly been seted up to the upper surface of placing the dish, the lower surface of placing the dish is equipped with the heating chamber, the internal surface in heating chamber is equipped with first heating rod.
The rear end both sides of placing the dish are equipped with high temperature chamber and cooling chamber respectively, the inside in high temperature chamber is equipped with the second heating rod, the inside in cooling chamber is equipped with the condenser pipe, the upper surface middle part in high temperature chamber and cooling chamber all is equipped with temperature monitor, all communicate through the transmission pipe between high temperature chamber and the placing the dish and between cooling chamber and the placing the dish, the surface middle part of transmission pipe is equipped with the micropump, the inside of amplification instrument body is all located in high temperature chamber and cooling chamber.
Preferably, heat dissipation holes are formed in the lower portions of two sides of the outer surface of the amplification instrument body.
Preferably, the outer surface of the placing tray is sleeved with a spacer sleeve, and the middle part of the inner bottom surface of the placing tray is provided with a communication hole.
Preferably, the controller is electrically connected with the first heating rod, the second heating rod and the condensing tube, the controller is electrically connected with the temperature monitoring probe and the temperature monitor, and the controller is electrically connected with the micropump.
Preferably, a fixing frame is sleeved on the outer surface of the micropump, and the fixing frame is respectively connected with the high-temperature cavity and the cooling cavity.
Preferably, the upper parts of the other sides of the high temperature cavity and the cooling cavity are respectively provided with a guide pipe, and the other ends of the guide pipes penetrate through the amplification instrument body and extend outwards.
Compared with the prior art, the utility model has the following beneficial effects:
according to the technical scheme, when the temperature is frequently regulated for a short time aiming at the PCR amplification instrument in the application process, the temperature control module can realize the temperature regulation of the sample tube in a cold and hot water preheating exchange mode, so that the phenomenon of low heat exchange efficiency caused by using a temperature conduction mode is avoided, the temperature change efficiency of the sample tube is facilitated to be quickened, and the amplification effect of DNA is maintained.
Drawings
FIG. 1 is a schematic diagram of the front side of a temperature control module for a 96-well PCR amplification apparatus according to the present utility model;
FIG. 2 is a schematic diagram of the rear side of a temperature control module for a 96-well PCR amplification apparatus according to the present utility model;
FIG. 3 is an expanded view of a sealing cover of a temperature control module for a 96-well PCR amplification apparatus according to the present utility model;
FIG. 4 is a split view of a placement tray of a temperature control module for a 96-well PCR amplification apparatus according to the present utility model;
FIG. 5 is a split and sectional view of a high temperature cavity of a temperature control module for a 96-well PCR amplification apparatus according to the present utility model;
FIG. 6 is a split and cut-away view of a placement tray of a temperature control module for a 96-well PCR amplification apparatus according to the present utility model.
In the figure: 1. an amplification instrument body; 2. a heat radiation hole; 3. a controller; 4. sealing cover; 5. a temperature monitoring probe; 6. placing a tray; 7. placing the hole; 8. a spacer sleeve; 9. a communication hole; 10. a heating chamber; 11. a first heating rod; 12. a high temperature chamber; 13. a second heating rod; 14. a cooling chamber; 15. a condensing tube; 16. a temperature monitor; 17. a transmission tube; 18. a micropump; 19. a fixing frame; 20. a catheter.
Detailed Description
The following description is presented to enable one of ordinary skill in the art to make and use the utility model. The preferred embodiments in the following description are by way of example only and other obvious variations will occur to those skilled in the art.
The temperature control module for the 96-hole PCR amplification instrument comprises an amplification instrument body 1, wherein a controller 3 is arranged on one side of the upper surface of the amplification instrument body 1, a groove is formed in the other side of the upper surface of the amplification instrument body 1, a sealing cover 4 is movably inserted into the amplification instrument body 1 through one side of the upper surface of the groove, a plurality of temperature monitoring probes 5 are uniformly arranged on the lower surface of the sealing cover 4, a placing disc 6 is arranged in the middle of the inner bottom surface of the groove of the upper surface of the amplification instrument body 1, a plurality of placing holes 7 are uniformly formed in the upper surface of the placing disc 6, a heating cavity 10 is formed in the lower surface of the placing disc 6, and a first heating rod 11 is arranged on the inner surface of the heating cavity 10.
The rear end both sides of placing the dish 6 are equipped with high temperature chamber 12 and cooling chamber 14 respectively, the inside in high temperature chamber 12 is equipped with second heating rod 13, the inside in cooling chamber 14 is equipped with condenser pipe 15, the upper surface middle part in high temperature chamber 12 and cooling chamber 14 all is equipped with temperature monitor 16, between high temperature chamber 12 and the placing the dish 6 and between cooling chamber 14 and the placing the dish 6 all through transmission pipe 17 intercommunication, the surface middle part of transmission pipe 17 is equipped with micropump 18, the inside of amplification instrument body 1 is all located to high temperature chamber 12 and cooling chamber 14.
During operation, first heating rod 11 in the control module control heating chamber 10 in the accessible controller 3 starts at first, this moment first heating rod 11 can be to placing the dish 6 and preheat, simultaneously accessible pipe 20 is to the inside of high temperature chamber 12 and cooling chamber 14 carries out the perfusion of water, after high temperature chamber 12 and cooling chamber 14 are full of water, can be through the control module control second heating rod 13 in the controller 3 and condenser pipe 15 start, under this circumstances second heating rod 13 will heat the water in the high temperature chamber 12, simultaneously condenser pipe 15 will cool down the water in the cooling chamber 14, this moment temperature monitor 16 will be to the temperature of high temperature chamber 12 and cooling chamber 14 respectively in the signal receiving module of controller 3 show through the controller 3, this moment can be with the sample pipe that is ready in proper order put into place hole 7 and use sealed lid 4 to place the dish 6 and seal up, temperature probe 5 will extend to the sample pipe in the condition of placing the sealing up 6 to seal up the lid 4 and carry out the temperature probe 5 and will extend to the sample pipe in the temperature pipe under the temperature monitor pipe that the temperature monitor 3 and the temperature monitor pipe that is in this moment and the temperature monitor pipe is to the temperature monitor pipe is 3 and the temperature monitor pipe is carried out the temperature monitor, can realize the temperature monitor and the temperature monitor is carried out the temperature monitor through the temperature monitor pipe is 3 and is carried out the temperature monitor and is carried out the temperature monitor and temperature monitor is carried out the temperature monitor through the temperature monitor panel 3 in the temperature monitor panel 3.
When the water in the high-temperature cavity 12 is heated to a certain temperature by the second heating rod 13, under the condition that the temperature in the sample tube needs to be changed and raised, the micro pump 18 connected with the high-temperature cavity 12 can be controlled to be started by the control module in the controller 3, at the moment, the micro pump 18 can be matched with the transmission tube 17 to extract the heated water in the high-temperature cavity 12 and transmit the heated water into the placing disc 6, at the moment, the placing disc 6 can be quickly raised under the injection of the heated water, so that the temperature of the sample tube is driven to rise, when the temperature needed by the sample tube needs to be lowered, the micro pump 18 connected with the cooling cavity 14 can be controlled to be started by the control module in the controller 3, at the moment, the micro pump 18 can be matched with the transmission tube 17 to extract the condensed water in the cooling cavity 14 and transmit the condensed water into the placing disc 6, simultaneously, the micropump 18 connected with the high-temperature cavity 12 is controlled by the control module in the controller 3 to be started, at the moment, the micropump 18 can cooperate with the transmission pipe 17 to extract heating water in the placing tray 6 and transmit the heating water into the high-temperature cavity 12 for reheating, at the moment, only condensed water exists in the placing tray 6, the temperature of the placing tray 6 can be quickly driven to be reduced under the cooling and heat absorption of the condensed water, so that the sample pipe is driven to realize cooling and heat exchange, and meanwhile, in the process of using cold and hot water to carry out heat exchange adjustment on the placing tray 6, the first heating rod 11 is in a state of continuously heating the placing tray 6, so that the placing tray 6 can keep a certain temperature for conveniently carrying out temperature adjustment.
Referring to the accompanying drawings 1-6 of the specification, heat dissipation holes 2 are formed in the lower parts of two sides of the outer surface of the amplification instrument body 1.
The heat dissipation of the amplification instrument body 1 is facilitated through the heat dissipation holes 2.
The outer surface of the placing plate 6 is sleeved with a spacer sleeve 8, and the middle part of the inner bottom surface of the placing plate 6 is provided with a communication hole 9.
The temperature of the placing tray 6 can be isolated through the isolating sleeve 8, and cold and hot water can circulate in the placing tray 6 conveniently through the communicating hole 9.
The controller 3 is electrically connected with the first heating rod 11, the second heating rod 13 and the condensing tube 15, the controller 3 is electrically connected with the temperature monitoring probe 5 and the temperature monitor 16, and the controller 3 is electrically connected with the micropump 18.
The temperature control module in the controller 3 can be conveniently controlled in an electric connection mode.
The outer surface of the micropump 18 is sleeved with a fixing frame 19, and the fixing frame 19 is respectively connected with the high-temperature cavity 12 and the cooling cavity 14.
The micropump 18 can be mounted and fixed by a fixing frame 19.
The upper parts of the other sides of the high temperature cavity 12 and the cooling cavity 14 are respectively provided with a conduit 20, and the other ends of the conduits 20 penetrate through the amplification instrument body 1 and extend outside.
The high temperature chamber 12 and the cooling chamber 14 can be filled with water and replaced by the pipe 20.
To sum up: the temperature control module is favorable for accelerating the temperature change efficiency of the sample tube so as to maintain the amplification effect of DNA.
The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made therein without departing from the spirit and scope of the utility model, which is defined by the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (6)
1. The temperature control module for the 96-hole PCR amplification instrument is characterized by comprising an amplification instrument body (1), wherein one side of the upper surface of the amplification instrument body (1) is provided with a controller (3), the other side of the upper surface of the amplification instrument body (1) is provided with a groove, the amplification instrument body (1) is movably inserted with a sealing cover (4) through one side of the upper surface of the groove, the lower surface of the sealing cover (4) is uniformly provided with a plurality of temperature monitoring probes (5), the middle part of the inner bottom surface of the groove on the upper surface of the amplification instrument body (1) is provided with a placing disc (6), the upper surface of the placing disc (6) is uniformly provided with a plurality of placing holes (7), the lower surface of the placing disc (6) is provided with a heating cavity (10), and the inner surface of the heating cavity (10) is provided with a first heating rod (11);
the utility model discloses a high temperature amplification instrument, including placing dish (6), including cooling chamber (14), high temperature chamber (12), cooling chamber (14), condenser pipe (15), temperature monitor (16) all are equipped with in the upper surface middle part of high temperature chamber (12) and cooling chamber (14), all communicate through transmission pipe (17) between high temperature chamber (12) and placing dish (6) and between cooling chamber (14) and placing dish (6), the surface middle part of transmission pipe (17) is equipped with micropump (18), the inside of amplification instrument body (1) is all located in high temperature chamber (12) and cooling chamber (14).
2. The temperature control module for the 96-well PCR amplification instrument of claim 1, wherein heat dissipation holes (2) are formed in the lower portions of two sides of the outer surface of the amplification instrument body (1).
3. The temperature control module for the 96-well PCR amplification instrument of claim 1, wherein the outer surface of the placing tray (6) is sleeved with a spacer sleeve (8), and the middle part of the inner bottom surface of the placing tray (6) is provided with a communication hole (9).
4. The temperature control module for the 96-well PCR amplification apparatus of claim 1, wherein the controller (3) is electrically connected to the first heating rod (11), the second heating rod (13) and the condenser tube (15), the controller (3) is electrically connected to the temperature monitoring probe (5) and the temperature monitor (16), and the controller (3) is electrically connected to the micropump (18).
5. The temperature control module for the 96-well PCR amplification instrument of claim 1, wherein a fixing frame (19) is sleeved on the outer surface of the micropump (18), and the fixing frame (19) is respectively connected with the high-temperature cavity (12) and the cooling cavity (14).
6. The temperature control module for the 96-well PCR amplification instrument according to claim 1, wherein the upper parts of the other sides of the high-temperature cavity (12) and the cooling cavity (14) are respectively provided with a guide pipe (20), and the other ends of the guide pipes (20) penetrate through the amplification instrument body (1) to extend outside.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321937719.9U CN220520480U (en) | 2023-07-22 | 2023-07-22 | Temperature control module for 96-hole PCR (polymerase chain reaction) amplification instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321937719.9U CN220520480U (en) | 2023-07-22 | 2023-07-22 | Temperature control module for 96-hole PCR (polymerase chain reaction) amplification instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220520480U true CN220520480U (en) | 2024-02-23 |
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ID=89928218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321937719.9U Active CN220520480U (en) | 2023-07-22 | 2023-07-22 | Temperature control module for 96-hole PCR (polymerase chain reaction) amplification instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220520480U (en) |
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2023
- 2023-07-22 CN CN202321937719.9U patent/CN220520480U/en active Active
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