CN209307381U - A kind of PCR temperature regulating device of low lift pump induction heating - Google Patents
A kind of PCR temperature regulating device of low lift pump induction heating Download PDFInfo
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- CN209307381U CN209307381U CN201821592774.8U CN201821592774U CN209307381U CN 209307381 U CN209307381 U CN 209307381U CN 201821592774 U CN201821592774 U CN 201821592774U CN 209307381 U CN209307381 U CN 209307381U
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Abstract
The utility model relates to a kind of PCR temperature regulating devices of low lift pump induction heating, described device includes: heating cavity 1, PCR reactor unit 2, hollow metal disk 3, coil 4, refrigeration unit 5, temperature control unit 6, magnetic induction heat control unit 7, main control unit 8, stepper motor 9, temperature sensor 10, wherein, PCR reactor unit 2 is located in heating cavity 1 between hollow metal disk 3, 2 disk periphery of hollow metal disk 3 and PCR reactor unit fits closely, magnetic induction heat control unit 7 is connect with coil 4, and grafting vertical with main control unit 8, temperature control unit 6 connects temperature sensor 10, with refrigeration unit 5, magnetic induction heat control unit 7 and main control unit 8 constitute heating-cooling cyclic control system of PCR reaction.
Description
Technical field:
The present invention relates to a kind of Metal-magnetic needle, in particular to a kind of suitable polymerase chain reaction alternating temperature PCR is anti-
The temperature regulating device answered.
Background technique:
Polymerase chain reaction (Polymerase Chain Reaction, PCR) is a kind of molecular biosciences of DNA cloning
Learn reaction.One of the key factor of alternating temperature PCR reaction success or not, in that temperature needed for must satisfy its reaction entity is followed
Ring condition, that is, high-temperature denaturation, low-temperature annealing, medium temperature primer extend so temperature cycles are its bases, and " become in temperature cycles
Temperature " rate will determine the bulk velocity of the specificity and PCR operation of PCR reaction.The heat circulating system of normal PCR is roughly divided into
Water-bath type, air-flowing type and semiconductor-type, and heating method therein is that resistance wire, resistor disc or semiconductor combine hot Gai Jiare
Deng the heat transfer of pyrotoxin later again heats test tube to heating device by heat transfer.Heating rate is in 2-3
DEG C/sec, the experiment of 30 circulations is completed, needs 2-3 hours.
Technical disadvantages: heat transfer series is more, and transfer efficiency is lower, and utilization rate is poor.
Summary of the invention:
The present invention proposes that one kind based on electromagnetic inductive heating principle, can be improved the temperature regulating device of PCR reaction Cooling rate,
Structure of the invention without upper cover cross-sectional view as shown in Fig. 2,
The present invention provides a kind of PCR temperature regulating device of low lift pump induction heating, and described device includes: heating cavity 1, PCR
Reactor unit 2, hollow metal disk 3, coil 4, refrigeration unit 5, temperature control unit 6, magnetic induction heat control unit 7,
Main control unit 8, stepper motor 9, temperature sensor 10, wherein PCR reactor unit 2 is located in heating cavity 1 and hollow gold
Belong between disk 3,2 disk periphery of hollow metal disk 3 and PCR reactor unit fits closely, magnetic induction heat control unit 7
It is connect with coil 4, and grafting vertical with main control unit 8, temperature control unit 6 connects temperature sensor 10, with refrigeration unit
5, magnetic induction heat control unit 7 and main control unit 8 constitute heating-cooling cyclic control system of PCR reaction.
The heating cavity 1, as shown in Fig. 2, it is mounted to the structure member between PCR reactor unit 2 and shell,
Effect is and instrument upper cover, hood 301 a cylindrical cavity to be formed, by 3 envelope of PCR reactor unit 2 and rosette
It closes in cavity, heating cavity uses heat-insulated PC material, prevents heating temperature from spreading, and guarantees heating rate.
The PCR reactor unit 2 can place 1 or more Reagent Tube as shown in Fig. 2, being reagent tube placement apparatus,
Its main material is almag AZ31B.
The hollow metal disk 3, as shown in figure 3, it is fitted closely with 2 disk periphery of PCR reactor unit, it is metal
Material components, the heater of temperature regulating device of the present invention, by main control unit 8 to magnetic induction heat control unit 7 and coil 4
Control, according to electromagnetic coupling theory, make inside hollow metal disk 3 generate eddy current effect and generate heat, and with PCR reactor unit
2 conduction of heat, to reach the heating to PCR reaction tube sample.
The coil 4, as shown in figure 4, around on 1 hollow circular inner wall of heating cavity, with magnetic induction computer heating control list
Member 7 connects, and is one of main composition of Metal-magnetic needle, and main control unit 8 passes through control magnetic induction heat control unit 7
So that coil 4 generates alternating magnetic field, according to electromagnetic coupling theory, makes to generate eddy current effect inside 3 body of hollow metal disk, make it
Generate spontaneous heating.
The refrigeration unit 5 passes through master as shown in Fig. 2, playing the role of fast cooling in PCR reaction temperature circulation
Control unit 8 opens or closes system according to PCR reaction temperature circulating-heating to the temperature of 6 real-time monitoring of temperature control unit
Cold unit 5.
The temperature control unit 6, as shown in figure 5, by hood 601, temperature signal collection module 602, upper shielding case
603, receiving coil 604, transmitting coil 605, lower shielding case 606 and processes temperature signal module 607 form.
The temperature control unit 6 passes through temperature sensor real-time monitoring as shown in Fig. 2, being connection temperature sensor 10
The Reagent Tube sample temperature put in PCR reactor unit 2, with refrigeration unit 5, magnetic induction heat control unit 7 and main control
Unit 8 constitutes heating-cooling loop control of PCR reaction.
The magnetic induction heat control unit 7, as shown in fig. 6, connect with coil 4, grafting vertical with main control unit 8,
It includes power switching modules, drive module, control driving coil 4 by main control unit 8.
The main control unit 8, as shown in fig. 6, comprising power module, Temperature Treatment module, the detection of magnetic strength heated current
Module, data transmission module, motion-control module, by refrigeration unit 5, temperature control unit 6, magnetic induction computer heating control list
The control of member 7 realizes that PCR reacts accurate temperature cycles control.
The present invention gives PCR reactor unit heated for controlling temperature using electromagnetic induction heater, which directly acts on
In PCR reactor unit, electromagnetic induction generation eddy-current technique thermal loss is small, and heating speed is fast, high conversion efficiency, and conversion ratio is most
Height can achieve 95%, in conjunction with accurately heating and temperature control algorithm, substantially increases the rate of heat addition of PCR, also improves PCR
The specificity of reaction.Heating rate completes the experiment of 30 circulations at 15-40 DEG C/sec within 30 minutes.
Present invention improves over the heating methods of PCR reaction, improve the PCR rate of heat addition.
The principle of electromagnetic induction technology heating of the invention is: being by DC inverter using semi-bridge inversion unit at certain
The alternating current of frequency makes the hollow gold in heating cavity 1 according to electromagnetic coupling theory using making alternating current generate alternating magnetic field
Belong to and generate the stream effect of vortex filament circle 4 inside 3 body of disk, achievees the purpose that spontaneous heating.
Metal-oxide-semiconductor is used about electromagnetic induction heating power switch, is the core devices in driving unit, passes through inverse parallel one
A Schottky diode carrys out metal-oxide-semiconductor during protective switch；Semi-bridge inversion unit selects encapsulation smaller, to peripheral components accuracy
The small highly integrated half-bridge of requirement, grid drive chip.
It is dead provided with Microsecond grade between the complementary output waveform of signal input part about magnetic induction heat driven chip
Area's time guarantees the normal work of semi-bridge inversion unit；Signal output end drives upper metal-oxide-semiconductor using bootstrap type, reaches
One chip drives two by all means, easily controllable, save the cost.
About the selection of the capacitor's capacity in oscillation circuit, on the one hand reach conducive to the magnetic induction heating temperature rise speed of unit
The requirement of PCR heating speed, is on the other hand conducive to energy conservation and environmental protection, and the conversion ratio of electromagnetic heating is made to reach maximum.
Power supply about magnetic induction heating uses denoising measure, is carrying out magnetic induction heating module relative to light current unit
For high-power heating while, the normal work of light current unit will not be interfered, thus more convenient with light current unit collection
In Cheng Yi integral device.
About inverter, use 48V for inverter, complementary drive PWM waveform is 25KHz, measures oscillation circuit
Waveform is that peak-to-peak value is 48V, and frequency is the triangular wave of complementary drive frequency.Compared to the heating method of normal PCR, heating speed is bright
Aobvious to accelerate, utilization rate of electrical increases.
Detailed description of the invention
Fig. 1 is that apparatus of the present invention implement control principle drawing
Fig. 2 is that apparatus of the present invention implement cross-sectional view of the structure
Fig. 3 is that the present invention implements 3 location drawing of hollow metal disk
Fig. 4 is that the present invention implements coil 4 in heating cavity mounting means figure
Fig. 5 is to implement 6 composition schematic diagram of temperature control unit in Fig. 2 of the present invention
Fig. 6 is that the present invention implements magnetic induction heat control unit, coil 4, main control unit mounting means schematic diagram
Fig. 7 is control flow chart of the present invention
Specific embodiment:
PCR temperature regulating device of the invention implements functional block diagram as shown in Figure 1, it implements structure as shown in Fig. 2, by adding
Hot cavity 1, PCR reactor unit 2, hollow metal disk 3, coil 4, refrigeration unit 5, temperature control unit 6, magnetic induction adds
Thermal control units 7, main control unit 8 form, and wherein PCR reactor unit 2 is placed in hollow metal disk 3, hollow metal circle
Disk 3 is mounted in heating cavity 1, and the marginal portion of heating cavity 1 is equipped with coil 4, refrigeration unit 5, temperature control unit 6, magnetic
Induction heating control unit 7, main control unit 8 be placed in heating cavity lower part, coil 4 and magnetic strength heat control unit 7 and
Main control unit 8 is connected to heating source.
7 external direct current power supply of main control unit, by the semi-bridge inversion unit on magnetic induction heat control unit 6 by direct current
Electricity is reverse into the alternating current of certain frequency, when high-frequency current passes through coil 4, around generate magnetic flux, alternating magnetic field and empty
3 impedance of heart rosette constitutes circuit and carries out electric discharge generation eddy current effect, and hollow metal disk 3 generates heat, with PCR reactor unit
By heat transfer, achieve the purpose that PCR reaction heating.
Due to faradic kelvin effect, the depth that induced current penetrates hollow metal disk 3 is limited, diathermanous layer
Depth is related with the working frequency of power supply, the resistivity of hollow metal disk 3 and magnetic permeability.Then hollow metal disk is in diathermanous layer
Induction heating can be realized and be directly heated within depth bounds, therefore hollow metal is close in reaction tube position in PCR reaction member 1
Disk 3 improves heating efficiency, reduces thermal losses.
The magnetic induction heat control unit 7 and main control unit 8 for induction heating control flow as shown in Figure 1,
Single-chip microcontroller exports two-way PWM waveform to control half-bridge driven chip, so that metal-oxide-semiconductor (half-bridge cells) be driven to carry out inversion；Half-bridge
Power supply of the alternating current as coil 4 is exported, oscillating unit generates alternating magnetic field, so that hollow metal disk 3 generates eddy current effect；
Electric current in current detection module real-time monitoring heating circuit adjusts PWM duty ratio in real time using pid control algorithm to regulate and control
The temperature of temperature rise speed, 6 pairs of temperature control unit adjustings carries out real-time monitoring.Change the duty ratio of complementation PWM by single-chip microcontroller
The transformation efficiency in electromagnetic induction circuit is adjusted, is set so that hollow metal disk 3 and PCR reactor unit 2 be made to reach
Temperature.
The frequency of PWM wave is set as 25KHz, when oscillating capacitance is 6.3uF, using Precise temperature gauge to metal hollow
Disk carries out heating test, power supply DC48V, and open environment temperature rise speed can reach PCR equipment pair up to 15-40 DEG C/S
The requirement of temperature rise speed substantially increases the reaction rate of PCR.
The experimental data of PCR temperature regulating device of the invention and the relatively more possessed advantage of existing apparatus is as follows:
| Heating method | Cooling rate | Reaction time | Volume and power consumption | |
| Apparatus of the present invention | Magnetic strength heating | 15-40℃/S | 30 minutes | It is small in size, it is low in energy consumption |
| Existing apparatus 1 | Peltier heating | 3-5℃/S | 2-3 hours | Bulky, power consumption is high |
Claims (9)
1. a kind of PCR temperature regulating device of low lift pump induction heating, which is characterized in that described device includes: heating cavity (1), PCR
Reactor unit (2), hollow metal disk (3), coil (4), refrigeration unit (5), temperature control unit (6), magnetic induction heating
Control unit (7), main control unit (8), stepper motor (9), temperature sensor (10), wherein PCR reactor unit (2) position
It is neutralized between hollow metal disk (3) in heating cavity (1), outside hollow metal disk (3) and PCR reactor unit (2) disk
Week fits closely, and magnetic induction heat control unit (7) is connect with coil (4), and grafting vertical with main control unit (8), temperature
Control unit (6) connects temperature sensor (10), with refrigeration unit (5), magnetic induction heat control unit (7) and main control unit
(8) heating-cooling cyclic control system of PCR reaction is constituted.
2. temperature regulating device according to claim 1, which is characterized in that wherein, heating cavity (1) is installed on PCR reactor
Between unit (2) and shell, PCR reactor unit (2) and hollow metal disk (3) are in heating cavity (1).
3. temperature regulating device according to claim 1, which is characterized in that wherein, PCR reactor unit (2) is that Reagent Tube is put
Device is set, 1 or more Reagent Tube can be placed.
4. temperature regulating device according to claim 1, which is characterized in that wherein, hollow metal disk (3) and PCR reactor
Unit (2) disk periphery fits closely, the control by main control unit (8) to magnetic induction heat control unit (7) and coil (4)
System makes to generate eddy current effect inside hollow metal disk (3) and generate heat, thermally conductive with PCR reactor unit (2), reacts PCR and tries
The heating of pipe sample.
5. temperature regulating device according to claim 1, which is characterized in that wherein, coil (4), around in heating cavity (1) sky
It on heart annular inner wall, is connect with magnetic induction heat control unit (7), main control unit (8) passes through control magnetic induction computer heating control
Unit (7) makes coil (4) to generate alternating magnetic field, and making hollow metal disk (3), portion generates eddy current effect in vivo, is produced from it
Fever.
6. temperature regulating device according to claim 1, which is characterized in that wherein, refrigeration unit (5) passes through main control unit
(8) to temperature control unit (6) real-time monitoring temperature, refrigeration unit (5) are opened or closed.
7. temperature regulating device according to claim 1, which is characterized in that wherein, temperature control unit (6), by hood
(601), temperature signal collection module (602), upper shielding case (603), lower shielding case (606), receiving coil (604), emission lines
It encloses (605) and processes temperature signal module (607) composition, temperature control unit (6) connects temperature sensor (10), in real time
Monitor the Reagent Tube sample temperature put in PCR reactor unit (2).
8. temperature regulating device according to claim 1, which is characterized in that wherein, magnetic induction heat control unit (7), with line
(4) connection is enclosed, grafting vertical with main control unit (8), it includes power switching modules, drive module, by main control unit (8)
It controls driving coil (4).
9. temperature regulating device according to claim 1, which is characterized in that wherein, main control unit (8) includes power module,
Temperature Treatment module, magnetic strength heated current detection module, data transmission module, motion-control module, by refrigeration unit
(5), the temperature cycles control of PCR reaction is realized in the control of temperature control unit (6), magnetic induction heat control unit (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821592774.8U CN209307381U (en) | 2018-09-28 | 2018-09-28 | A kind of PCR temperature regulating device of low lift pump induction heating |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821592774.8U CN209307381U (en) | 2018-09-28 | 2018-09-28 | A kind of PCR temperature regulating device of low lift pump induction heating |
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| CN209307381U true CN209307381U (en) | 2019-08-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108998371A (en) * | 2018-09-28 | 2018-12-14 | 北京金豪制药股份有限公司 | A kind of PCR temperature regulating device of low lift pump induction heating |
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2018
- 2018-09-28 CN CN201821592774.8U patent/CN209307381U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108998371A (en) * | 2018-09-28 | 2018-12-14 | 北京金豪制药股份有限公司 | A kind of PCR temperature regulating device of low lift pump induction heating |
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