CN219567952U - Temperature control device of PCR (polymerase chain reaction) amplification instrument - Google Patents

Abstract

The utility model belongs to the technical field of PCR auxiliary tools, and particularly relates to a temperature control device of a PCR amplification instrument, which comprises a box body and a box cover arranged above the box body, wherein one side of the box cover is hinged with the top of the box body, the bottom in the box body is provided with a temperature controller, a heat conducting plate is attached above the temperature controller, a plurality of matrix-type distributed placing grooves are formed in the heat conducting plate, a sample tube is movably inserted in each placing groove, a plurality of heat transfer fins are fixedly arranged on the upper end face of the heat conducting plate, and the heat transfer fins are positioned among the sample tubes; the temperature controller comprises an input pipe, an output pipe and a coil pipe, wherein the coil pipe is spirally arranged in the box body, and the output pipe and the input pipe are respectively communicated with two ends of the coil pipe. The defect of the prior art is overcome, fluid with proper temperature is input to the temperature controller through the heating box, low-temperature fluid is input to the temperature controller through the cooling box when the temperature is reduced, rapid temperature reduction is realized, the time consumption of temperature control is reduced, and the efficiency and the precision of the temperature control are maintained.

Description

Temperature control device of PCR (polymerase chain reaction) amplification instrument

Technical Field

The utility model belongs to the technical field of PCR auxiliary tools, and particularly relates to a temperature control device of a PCR amplification instrument.

Background

PCR is a molecular biological technique for amplifying a specific DNA fragment, which uses DNA that is denatured at about 95℃and is decomposed into two single-stranded DNAs from double-stranded DNA; when the temperature of the DNA decomposed into single strands is reduced to about 60 ℃, the single-stranded DNA is combined with the primer; the DNA combined with the primer is subjected to semi-reserved replication according to the base complementary pairing principle under the action of DNA polymerase, and double DNA can be obtained after replication is completed. The DNA is amplified in large quantity through the circulation of the temperature at 95 ℃ and 60 ℃ for a plurality of times.

Patent CN111346685a discloses a device and a method capable of realizing rapid temperature control, which adopts a fluid with proper temperature to directly enter a temperature control cavity to replace the original fluid, so as to realize rapid temperature control without gradually heating or cooling the temperature control cavity through a temperature control component, thereby reducing the time required by the temperature raising and cooling processes in the temperature control process and improving the rate of temperature control.

According to the technical scheme, the original fluid is replaced by entering the temperature control cavity through the fluid with the proper temperature, a certain time is consumed in the process, the uniformity and the efficiency of fluid replacement are difficult to ensure, and time consumption is easy to increase or the temperature control precision is easy to decrease.

Disclosure of Invention

The utility model aims to provide a temperature control device of a PCR amplification instrument, which overcomes the defects of the prior art, fluid with proper temperature is input into a temperature controller through a heating box, low-temperature fluid is input into the temperature controller through a cooling box during cooling, rapid cooling is realized, the time consumption of temperature control is reduced, and the efficiency and the accuracy of temperature control are maintained.

In order to solve the problems, the technical scheme adopted by the utility model is as follows:

the utility model provides a PCR amplification appearance temperature control device, includes the box body and sets up the lid in the box body top, and one side of lid is articulated with the top of box body, the bottom is provided with the temperature controller in the box body, and the top laminating of temperature controller has the heat conduction board, a plurality of matrix distribution's standing grooves have been seted up on the heat conduction board, and the activity has pegged graft in every standing groove has the sample pipe, the up end of heat conduction board has set firmly a plurality of heat transfer fins, and heat transfer fin is located between the sample pipe.

Further, the temperature controller comprises an input pipe, an output pipe and a coil pipe, wherein the coil pipe is spirally arranged in the box body, the output pipe and the input pipe are respectively communicated with two ends of the coil pipe, a heating box and a cooling box are respectively arranged on two sides of the box body, an output pump and an input pump are respectively arranged on two sides of the cooling box and two sides of the heating box, and the output pump and the input pump are connected with the output pipe and the input pipe through connecting pipes.

Further, electromagnetic valves are arranged at two ends of the input pipe and the output pipe.

Further, an electric heater is arranged in the heating box, a semiconductor refrigerator is arranged in the cooling box, temperature sensors are arranged in the heating box and the cooling box, and heat conduction liquid is filled in the heating box and the cooling box.

Further, the top of sample tube is provided with sealed lid, temperature probe is installed to sealed lower terminal surface, and is provided with the connecting rod of being connected with temperature probe in the sealed lid, the top of connecting rod is connected with the contact of metal material, a plurality of connection pieces that correspond with the contact are installed to the lower terminal surface of lid.

Further, the box body and the box cover are filled with heat insulation materials.

Compared with the prior art, the utility model has the following beneficial effects:

1. according to the utility model, the temperature of the temperature controller is improved by inputting the fluid with proper temperature into the temperature controller through the heating box, and the low-temperature fluid is input into the temperature controller through the cooling box during cooling, so that rapid cooling is realized, the time consumption of temperature control is reduced, and the efficiency and the accuracy of temperature control are maintained.

2. According to the utility model, heat is transmitted into the box body through the heat-conducting plate and the heat-conducting fins to heat and cool the sample tube, and the temperature in the sample tube is monitored through the temperature probe.

Drawings

FIG. 1 is a schematic diagram of a temperature control device of a PCR amplification apparatus.

FIG. 2 is a schematic diagram showing the internal structure of a temperature control device of a PCR amplification unit.

FIG. 3 is an enlarged schematic view of the temperature control device of the PCR amplification unit.

FIG. 4 is a top view of a temperature control device of a PCR amplification unit.

FIG. 5 is a schematic diagram showing the structure of a temperature controller in a temperature control device of a PCR amplification apparatus.

In the figure: 1. a case body; 2. a box cover; 3. a heating box; 4. a cooling box; 5. an output pump; 6. a connecting pipe; 7. a temperature controller; 71. an input tube; 72. a coiled pipe; 73; solenoid valve, 74, output tube; 8. a heat conductive plate; 9. a heat transfer fin; 10. a sample tube; 11. sealing cover; 12. a temperature probe; 13. a connecting rod; 14. electric shock; 15. a connecting sheet; 16. a placement groove; 17. and (5) inputting a pump.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1-5, the temperature control device of the PCR amplification instrument of the present utility model comprises a box body 1 and a box cover 2 arranged above the box body 1, wherein one side of the box cover 2 is hinged with the top of the box body 1, a temperature controller 7 is arranged in the bottom of the box body 1, a heat conducting plate 8 is attached to the upper side of the temperature controller 7, a plurality of matrix-distributed placing grooves 16 are formed in the heat conducting plate 8, a sample tube 10 is movably inserted in each placing groove 16, a plurality of heat transfer fins 9 are fixedly arranged on the upper end surface of the heat conducting plate 8, and the heat transfer fins 9 are positioned between the sample tubes 10.

The temperature controller 7 comprises an input pipe 71, an output pipe 74 and a coil pipe 72, the coil pipe 72 is spirally arranged in the box body 1, the output pipe 74 and the input pipe 71 are respectively communicated with two ends of the coil pipe 72, the heating box 3 and the cooling box 4 are respectively arranged at two sides of the box body 1, the output pump 5 and the input pump 17 are respectively arranged at two sides of the cooling box 4 and the heating box 3, and the output pump 5 and the input pump 17 are connected with the output pipe 74 and the input pipe 71 through connecting pipes 6.

To control the flow direction of the fluid, solenoid valves 73 are installed at both ends of the input pipe 71 and the output pipe 74.

In order to be convenient for rise and fall, be provided with electric heater in the heating cabinet 3, be provided with the semiconductor refrigerator in the cooling cabinet 4, all be provided with temperature sensor in heating cabinet 3 and the cooling cabinet 4, and all fill heat conduction liquid in heating cabinet 3 and the cooling cabinet 4.

In order to monitor the temperature inside the sample tube 10, the top of the sample tube 10 is provided with a sealing cover 11, the lower end face of the sealing cover 11 is provided with a temperature probe 12, a connecting rod 13 connected with the temperature probe 12 is arranged in the sealing cover 11, the top end of the connecting rod 13 is connected with a contact point of a metal material, and the lower end face of the box cover 2 is provided with a plurality of connecting pieces 15 corresponding to the contact point.

In order to avoid heat loss, the box body 1 and the box cover 2 are filled with heat insulation materials; the heat-insulating material is filled to isolate the heat exchange between the inside and the outside of the box body 1, so that heat loss is avoided, and the accuracy of temperature adjustment is improved.

In summary, according to the temperature control device of the PCR amplification apparatus of the present utility model, when in use, the sample tube 10 with the sample is inserted into the placement groove 16 on the heat conducting plate 8, and then the sealing cover 11 is connected with the sample tube 10, and at this time, the temperature probe 12 is inserted into the sample tube 10 for detecting the temperature in the sample tube 10; in the process of PCR amplification, the temperature of the fluid is increased to a required temperature through the heating box 3, then the output pump 5 is started to input the hot fluid into the input pipe 71 and the coil 72 from the connecting pipe 6, at the moment, the two electromagnetic valves 73 on one side of the cooling box 4 are closed, and the hot fluid transfers heat to the sample pipe 10 through the heat conducting plate 8 and the heat conducting fins 9 after entering the coil 72, so that the sample is heated until the sample reaches a set temperature; when the temperature is reduced, low-temperature fluid is input into the coil pipe 72 through the cooling box 4, the temperature of the heating box 3 is reduced to a preset temperature at the moment, when the temperature of the sample tube 10 is reduced to the preset temperature by the low-temperature fluid, the cooling box 4 is closed again, the fluid heated by the heating box 3 is input into the temperature controller 7, and the temperature accuracy of the sample tube 10 is maintained while the rapid temperature reduction is ensured.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (6)

1. A temperature control device of a PCR amplification instrument is characterized in that: including box body (1) and lid (2) of setting in box body (1) top, and one side of lid (2) is articulated with the top of box body (1), be provided with temperature controller (7) in box body (1), and the top laminating of temperature controller (7) has heat conduction board (8), set up standing groove (16) that a plurality of matrix distributions on heat conduction board (8), and every standing groove (16) internal activity grafting has sample tube (10), a plurality of heat transfer fins (9) have set firmly to the up end of heat conduction board (8), and heat transfer fins (9) are located between sample tube (10).

2. The temperature control device for a PCR amplification unit according to claim 1, wherein: temperature controller (7) are including input tube (71), output tube (74) and coil pipe (72), coil pipe (72) are the heliciform setting in box body (1), and output tube (74) and input tube (71) communicate with the both ends of coil pipe (72) respectively, heating cabinet (3) and cooling cabinet (4) are installed respectively to the both sides of box body (1), output pump (5) and input pump (17) are installed respectively to the both sides of cooling cabinet (4) and heating cabinet (3), and output pump (5) and input pump (17) are connected with output tube (74) and input tube (71) through connecting pipe (6).

3. The temperature control device for a PCR amplification unit according to claim 2, wherein: electromagnetic valves (73) are arranged at two ends of the input pipe (71) and the output pipe (74).

4. The temperature control device for a PCR amplification unit according to claim 2, wherein: an electric heater is arranged in the heating box (3), a semiconductor refrigerator is arranged in the cooling box (4), temperature sensors are arranged in the heating box (3) and the cooling box (4), and heat conduction liquid is filled in the heating box (3) and the cooling box (4).

5. The temperature control device for a PCR amplification unit according to claim 1, wherein: the top of sample tube (10) is provided with sealed lid (11), temperature probe (12) are installed to the lower terminal surface of sealed lid (11), and are provided with connecting rod (13) that are connected with temperature probe (12) in sealed lid (11), the top of connecting rod (13) is connected with the contact of metal material, a plurality of connection piece (15) that correspond with the contact are installed to the lower terminal surface of lid (2).

6. The temperature control device for a PCR amplification unit according to claim 1, wherein: the box body (1) and the box cover (2) are filled with heat insulation materials.