CN220520507U - Porous PCR amplification appearance samming temperature control module - Google Patents
Porous PCR amplification appearance samming temperature control module Download PDFInfo
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- CN220520507U CN220520507U CN202321953934.8U CN202321953934U CN220520507U CN 220520507 U CN220520507 U CN 220520507U CN 202321953934 U CN202321953934 U CN 202321953934U CN 220520507 U CN220520507 U CN 220520507U
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- pcr amplification
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- 238000012408 PCR amplification Methods 0.000 title claims abstract description 22
- 238000012544 monitoring process Methods 0.000 claims abstract description 21
- 239000000523 sample Substances 0.000 claims abstract description 21
- 238000010438 heat treatment Methods 0.000 claims abstract description 20
- 239000004065 semiconductor Substances 0.000 claims abstract description 18
- 238000005192 partition Methods 0.000 claims abstract description 10
- 238000005057 refrigeration Methods 0.000 claims description 10
- 230000003321 amplification Effects 0.000 abstract description 14
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 14
- 230000002349 favourable effect Effects 0.000 abstract description 4
- 239000013589 supplement Substances 0.000 abstract description 4
- 230000008859 change Effects 0.000 abstract description 3
- 230000001105 regulatory effect Effects 0.000 description 8
- 238000001816 cooling Methods 0.000 description 7
- 230000033228 biological regulation Effects 0.000 description 6
- 230000004544 DNA amplification Effects 0.000 description 5
- 230000001276 controlling effect Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000007859 qualitative PCR Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- Cooling Or The Like Of Semiconductors Or Solid State Devices (AREA)
Abstract
The utility model relates to the technical field of temperature control modules, in particular to a porous PCR amplification instrument temperature equalizing temperature control module which comprises a placing disc, wherein a temperature equalizing cavity is arranged on the lower surface of the placing disc, temperature monitoring probes are arranged on the upper parts of four sides of the inner surface of the temperature equalizing cavity, semiconductor refrigerating sheets are arranged in the middle of four sides of the inner surface of the temperature equalizing cavity, the semiconductor refrigerating sheets are arranged clockwise, a controller is arranged in the middle of the front end of the outer surface of the temperature equalizing cavity, an adjusting cavity is arranged on the lower surface of the temperature equalizing cavity, a plurality of partition boards are arranged in the adjusting cavity, a heating pipe and a refrigerating pipe are respectively arranged above and below the partition boards, and a plurality of adjusting fluted discs are uniformly arranged in the middle of the front end of the outer surface of the adjusting cavity. The device has reasonable design, is favorable for improving the adjustment efficiency of the temperature cycle change of the amplification instrument in a short time, can supplement the temperature after the temperature adjustment is finished, and is favorable for controlling the amplification instrument in the temperature equalization state after the temperature adjustment.
Description
Technical Field
The utility model relates to the technical field of temperature control modules, in particular to a porous PCR amplification instrument temperature equalizing temperature control module.
Background
The PCR amplification instrument is an instrument for amplifying specific DNA by Polymerase Chain Reaction (PCR), and is widely used for scientific research, clinical gene amplification, qualitative PCR gene amplification, fluorescent/enzyme endpoint-free quantitative DNA gene amplification, gene amplification of other gene analysis applications such as gene chips, and the like.
The PCR amplification instrument has the working principle that the purpose of gene amplification is realized by controlling the reaction temperature, and the primary amplification needs to be performed through 3 steps of high-temperature denaturation, low-temperature annealing and medium-temperature extension. Therefore, the temperature control module in the PCR amplification apparatus needs to perform cyclic adjustment of temperature change in a short time. However, the temperature adjustment efficiency of the temperature control module in the existing PCR amplification instrument is low, and the temperature is gradually reduced in the use process, so that the temperature equalization is inconvenient to maintain. For this reason, there is a need to propose an improvement for solving the above-mentioned problems.
Disclosure of Invention
The utility model aims to solve the defects in the prior art, and provides a uniform temperature control module of a porous PCR (polymerase chain reaction) amplification instrument, which can rapidly regulate the temperature in the amplification instrument, and can supplement the uniform temperature after the temperature regulation is finished, thereby being beneficial to controlling the amplification instrument in a uniform temperature state after the temperature regulation.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a porous PCR amplification appearance samming temperature control module, includes places the dish, the lower surface of placing the dish is equipped with samming chamber, samming chamber internal surface four-sided upper portion all is equipped with temperature monitoring probe, samming chamber internal surface four-sided middle part all is equipped with the semiconductor refrigeration piece, the semiconductor refrigeration piece is clockwise setting, samming chamber surface's front end middle part is equipped with the controller.
The lower surface in samming chamber is equipped with adjusts the chamber, the inside in adjusting the chamber is equipped with a plurality of baffles, the top and the below of baffle are equipped with heating pipe and refrigeration pipe respectively, the front end middle part of adjusting the chamber surface evenly is equipped with a plurality of regulation fluted disc, the middle part that the regulation fluted disc is close to adjusting chamber one side all is the bulge form and runs through the one end middle part of adjusting chamber and baffle and be connected, the other end middle part of baffle passes through bearing swing joint with the internal surface rear end in adjusting the chamber, the surface cover of adjusting the fluted disc is equipped with the drive belt, the internal surface of drive belt evenly be equipped with a plurality of meshing teeth with adjusting the fluted disc looks interlock, one of them the one side middle part that the regulation fluted disc kept away from adjusting the chamber is equipped with the motor.
The controller is electrically connected with the temperature monitoring probe and the semiconductor refrigerating sheet, and the controller is electrically connected with the heating pipe, the refrigerating pipe and the motor.
Preferably, the upper surface of the placing plate is uniformly provided with a plurality of placing grooves.
Preferably, the connection end of the temperature monitoring probe penetrates through the temperature equalizing cavity and extends out of the temperature equalizing cavity, and the connection end of the temperature monitoring probe is provided with a display disc.
Preferably, both ends of the heating pipe and the refrigerating pipe are provided with fixed lugs together, and the other ends of the fixed lugs are connected with the partition plate.
Preferably, a fixing frame is sleeved on the outer surface of the motor, and the other end of the fixing frame is connected with the outer surface of the adjusting cavity.
Compared with the prior art, the utility model has the following beneficial effects:
according to the technical scheme, in the application process, in the process of carrying out temperature control adjustment on the PCR amplification instrument, the temperature control module can be used for rapidly carrying out temperature adjustment on the interior of the amplification instrument, so that the adjustment efficiency of temperature cycling of the amplification instrument in a short time is improved, and meanwhile, the temperature equalization temperature can be supplemented after the temperature adjustment is finished, the amplification instrument is controlled in an even temperature state after the temperature adjustment, and the use effect of the amplification instrument is kept.
Drawings
FIG. 1 is a schematic diagram of a front side of a temperature equalization module of a multi-hole PCR amplification apparatus according to the present utility model;
FIG. 2 is a schematic diagram of a rear side of a temperature equalization module of a multi-hole PCR amplification apparatus according to the present utility model;
FIG. 3 is a split view of a placement tray of a temperature equalization temperature control module of a multi-hole PCR amplification instrument according to the present utility model;
FIG. 4 is a split view of a temperature equalizing cavity of a temperature equalizing temperature control module of a multi-hole PCR amplification instrument according to the present utility model;
FIG. 5 is a diagram showing the disassembly of the adjusting cavity of the temperature equalizing and controlling module of the multi-hole PCR amplification apparatus;
FIG. 6 is a diagram showing the separation of a partition plate of a temperature equalizing and controlling module of a multi-hole PCR amplification apparatus according to the present utility model.
In the figure: 1. placing a tray; 2. a placement groove; 3. a temperature equalizing cavity; 4. a temperature monitoring probe; 5. a display panel; 6. a semiconductor refrigeration sheet; 7. a controller; 8. a regulating chamber; 9. a partition plate; 10. a fixed ear; 11. heating pipes; 12. a refrigeration tube; 13. adjusting the fluted disc; 14. a transmission belt; 15. a motor; 16. and a fixing frame.
Detailed Description
The following description is presented to enable one of ordinary skill in the art to make and use the utility model. The preferred embodiments in the following description are by way of example only and other obvious variations will occur to those skilled in the art.
The utility model provides a porous PCR amplification appearance samming temperature control module as shown in fig. 1-6, includes places dish 1, and the lower surface of placing dish 1 is equipped with samming chamber 3, and the upper portion of samming chamber 3 internal surface four sides all is equipped with temperature monitoring probe 4, and the four sides middle part of samming chamber 3 internal surface all is equipped with semiconductor refrigeration piece 6, and semiconductor refrigeration piece 6 is clockwise setting, and the front end middle part of samming chamber 3 surface is equipped with controller 7.
The lower surface of samming chamber 3 is equipped with adjusts chamber 8, the inside of adjusting chamber 8 is equipped with a plurality of baffles 9, the top and the below of baffle 9 are equipped with heating pipe 11 and refrigeration pipe 12 respectively, the front end middle part of adjusting chamber 8 surface evenly is equipped with a plurality of regulation fluted disc 13, the middle part that adjusts fluted disc 13 is close to adjusting chamber 8 one side all is the bulge form and runs through adjusting chamber 8 and baffle 9's one end middle part and be connected, baffle 9's the other end middle part passes through bearing swing joint with adjusting chamber 8's internal surface rear end, the surface cover of adjusting fluted disc 13 is equipped with drive belt 14, the internal surface of drive belt 14 evenly is equipped with a plurality of meshing teeth with adjusting fluted disc 13 looks interlock, one of them adjusting fluted disc 13 is kept away from adjusting chamber 8's one side middle part and is equipped with motor 15.
The controller 7 is electrically connected with the temperature monitoring probe 4 and the semiconductor refrigerating sheet 6, and the controller 7 is electrically connected with the heating pipe 11, the refrigerating pipe 12 and the motor 15.
During operation, one of the regulating fluted discs 13 can be driven to rotate clockwise and anticlockwise through the motor 15, when the regulating fluted disc 13 is in a anticlockwise rotating state, the rest of the regulating fluted discs 13 are synchronously driven to rotate anticlockwise under the cooperation of the driving belt 14, at the moment, the partition plate 9 synchronously rotates to an upward state of the heating pipe 11 along with the rotation of the regulating fluted disc 13, when one of the regulating fluted discs 13 is in a clockwise rotating state, the rest of the regulating fluted discs 13 are synchronously driven to rotate clockwise under the cooperation of the driving belt 14, at the moment, the partition plate 9 synchronously rotates to an upward state of the cooling pipe 12 along with the rotation of the regulating fluted disc 13, at the moment, the heating pipe 11 or the cooling pipe 12 can be controlled to be started through a control module in the controller 7, and the bottom of the placing disc 1 is heated or cooled, so that the temperature of the amplification instrument can be quickly adjusted in a short time.
The temperature of the periphery of the placing tray 1 can be monitored through the temperature monitoring probe 4 in the heating and cooling process, when the temperature monitoring probe 4 monitors that the heating pipe 11 heats the placing tray 1 to a temperature suitable for operation, the temperature monitoring probe 4 can transmit signals into the signal receiving module in the controller 7, at this time, the controller 7 can control the heating pipe 11 to stop heating according to the received signals through the control module, and meanwhile, the control module is utilized to control the starting of the semiconductor refrigerating sheet 6, as the semiconductor refrigerating sheet 6 in the uniform temperature cavity 3 is arranged in a clockwise state, the heating end of the single semiconductor refrigerating sheet 6 is in a similar state with the heating end of the single semiconductor refrigerating sheet 6, and under the condition, the cold and hot temperatures of the semiconductor refrigerating sheet 6 can be in a mixed state, so that the bottom of the placing tray 1 is subjected to temperature supplement in a suitable uniform temperature state, and the placing groove 2 on the placing tray 1 is kept to be operated in a stable uniform temperature state.
Meanwhile, when the temperature monitoring probe 4 monitors that the cooling pipe 12 cools the placing tray 1 to a temperature suitable for operation, the temperature monitoring probe 4 can transmit signals into the signal receiving module in the controller 7, and at the moment, the controller 7 can control the cooling pipe 12 to stop cooling according to the received signals through the control module and control the semiconductor cooling sheet 6 to supplement the temperature of the bottom of the placing tray 1, so that the placing groove 2 on the placing tray 1 is kept to be in a stable uniform temperature state for operation.
Referring to the accompanying drawings 1-6 of the specification, a plurality of placement grooves 2 are uniformly formed in the upper surface of the placement plate 1.
The amplification tube can be placed through the placement tank 2.
The connecting end of the temperature monitoring probe 4 penetrates through the temperature equalizing cavity 3 and extends outside, and the connecting end of the temperature monitoring probe 4 is provided with a display disc 5.
The display disc 5 is electrically connected with the temperature monitoring probe 4, so that the temperature in the temperature equalizing cavity 3 monitored by the temperature monitoring probe 4 can be displayed.
Both ends of the heating pipe 11 and the refrigerating pipe 12 are provided with fixing lugs 10 together, and the other ends of the fixing lugs 10 are connected with the partition plate 9.
The heating pipe 11 and the refrigerating pipe 12 can be conveniently assembled and disassembled relative to the partition plate 9 through the fixing lugs 10.
The outer surface of the motor 15 is sleeved with a fixing frame 16, and the other end of the fixing frame 16 is connected with the outer surface of the adjusting cavity 8.
The mounting and dismounting of the motor 15 relative to the adjusting cavity 8 can be conveniently controlled through the fixing frame 16.
To sum up: the temperature control module is favorable for improving the adjustment efficiency of the temperature cycle change of the amplification instrument in a short time, and is favorable for controlling the amplification instrument in a uniform temperature state after temperature adjustment.
The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made therein without departing from the spirit and scope of the utility model, which is defined by the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (5)
1. The utility model provides a porous PCR amplification appearance samming temperature control module, its characterized in that, including placing dish (1), the lower surface of placing dish (1) is equipped with samming chamber (3), the upper portion of samming chamber (3) internal surface four sides all is equipped with temperature monitoring probe (4), the four side middle parts of samming chamber (3) internal surface all are equipped with semiconductor refrigeration piece (6), semiconductor refrigeration piece (6) are clockwise set up, the front end middle part of samming chamber (3) surface is equipped with controller (7);
the lower surface of the uniform temperature cavity (3) is provided with an adjusting cavity (8), the inside of the adjusting cavity (8) is provided with a plurality of clapboards (9), a heating pipe (11) and a refrigerating pipe (12) are respectively arranged above and below the clapboards (9), the middle part of the front end of the outer surface of the adjusting cavity (8) is uniformly provided with a plurality of adjusting fluted discs (13), the middle part of one side of the adjusting fluted disc (13) close to the adjusting cavity (8) is in a convex shape and penetrates through the adjusting cavity (8) to be connected with the middle part of one end of the clapboards (9), the middle part of the other end of the clapboards (9) is movably connected with the rear end of the inner surface of the adjusting cavity (8) through a bearing, the outer surface of the adjusting fluted disc (13) is sleeved with a driving belt (14), and the inner surface of the driving belt (14) is uniformly provided with a plurality of meshing teeth meshed with the adjusting fluted disc (13), and one side middle part of the adjusting fluted disc (13) far away from the adjusting cavity (8) is provided with a motor (15).
The controller (7) is electrically connected with the temperature monitoring probe (4) and the semiconductor refrigerating sheet (6), and the controller (7) is electrically connected with the heating pipe (11), the refrigerating pipe (12) and the motor (15).
2. The uniform temperature control module of the multi-hole PCR amplification instrument according to claim 1, wherein a plurality of placing grooves (2) are uniformly formed in the upper surface of the placing plate (1).
3. The homogeneous temperature control module of the multi-hole PCR amplification instrument according to claim 1, wherein the connecting end of the temperature monitoring probe (4) extends outside through the homogeneous temperature cavity (3), and the connecting end of the temperature monitoring probe (4) is provided with a display disc (5).
4. The porous PCR amplification apparatus temperature equalizing temperature control module according to claim 1, wherein both ends of the heating pipe (11) and the refrigerating pipe (12) are provided with fixing lugs (10) together, and the other ends of the fixing lugs (10) are connected with the partition plate (9).
5. The homogeneous temperature control module of the multi-hole PCR amplification instrument according to claim 1, wherein a fixing frame (16) is sleeved on the outer surface of the motor (15), and the other end of the fixing frame (16) is connected with the outer surface of the adjusting cavity (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321953934.8U CN220520507U (en) | 2023-07-24 | 2023-07-24 | Porous PCR amplification appearance samming temperature control module |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321953934.8U CN220520507U (en) | 2023-07-24 | 2023-07-24 | Porous PCR amplification appearance samming temperature control module |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220520507U true CN220520507U (en) | 2024-02-23 |
Family
ID=89938827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321953934.8U Active CN220520507U (en) | 2023-07-24 | 2023-07-24 | Porous PCR amplification appearance samming temperature control module |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220520507U (en) |
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2023
- 2023-07-24 CN CN202321953934.8U patent/CN220520507U/en active Active
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