CN209178400U - A kind of PCR instrument with sealer function - Google Patents
A kind of PCR instrument with sealer function Download PDFInfo
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- CN209178400U CN209178400U CN201821823484.XU CN201821823484U CN209178400U CN 209178400 U CN209178400 U CN 209178400U CN 201821823484 U CN201821823484 U CN 201821823484U CN 209178400 U CN209178400 U CN 209178400U
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- temperature
- heating
- cover board
- sealer
- pcr instrument
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Abstract
The utility model provides a kind of PCR instrument with sealer function, including metal heat-conducting block, lower temperature-controlling component and upper heating component, lower temperature-controlling component includes cooling piece, radiator and the first temperature sensor, upper heating component includes heating cover board, heating elements and second temperature sensor, in sealer, the temperature of the heating cover board is 100-200 DEG C, and the temperature of the metal heat-conducting block is 0-100 DEG C；In PCR reaction, the temperature of the heating cover board is 55-105 DEG C, and the temperature of the metal heat-conducting block is 0-100 DEG C.This PCR instrument has both sealer function and PCR function, has saved laboratory deployment cost, has reduced laboratory operating procedures；When upper heating assembly high-temperature sealer, lower temperature-controlling component provides cryo-conservation environment for sample orifice plate, effectively prevent top sealer high temperature that sample is caused to be denaturalized；When PCR reacts, upper heating component can provide the temperature for slightly above descending temperature-controlling component PCR to react, and upper heat and lower cold can be effectively prevented sample and volatilize upwards.
Description
Technical field
The utility model relates to a kind of Biological Detection technical fields, and in particular to a kind of PCR instrument with sealer function.
Background technique
Polymerase chain reaction (PCR) is a kind of for amplifying the Protocols in Molecular Biology for expanding specific DNA fragmentation.
PCR is that 95 DEG C of high temperature time variations will become single-stranded in vitro using DNA, and primer is pressed with single-stranded when low temperature (usually 60 DEG C or so)
The principle of base pair complementarity combines, then temperature regulating is to archaeal dna polymerase optimal reactive temperature (72 DEG C or so), archaeal dna polymerase edge
Direction composition complementary strand of the phosphoric acid to pentose.The implementation of PCR generally require rely on PCR instrument to the temperature of each stage of reaction and
Heating duration is controlled.
The cross contamination of volatilization and sample, can seal one on sample orifice plate after sample is heated in PCR reaction in order to prevent
Layer film is by specimen enclosure in respective tube body.At present in experiment, usually first sample orifice plate is sealed on sealer instrument
Then film carries out amplification reaction in PCR instrument again, laboratory needs while purchasing two kinds of instruments of sealer instrument and PCR instrument, increases
Laboratory deployment cost occupies lab space, and more disadvantageously general sealer instrument is all by high fever that film is warm
It is pressed in sample orifice surface, excessively high sealer temperature will cause sample denaturation, to influence PCR experiment result.
Utility model content
In order to overcome the above-mentioned deficiencies of the prior art, the purpose of the utility model is to provide a kind of with sealer function
PCR instrument.
In order to achieve the above objectives, the technical scheme adopted by the utility model to solve the technical problem is as follows: a kind of band sealer
The PCR instrument of function, comprising:
Metal heat-conducting block, block are equipped with the hole slot for placing sample orifice plate；
Lower temperature-controlling component comprising cooling piece, radiator and the first temperature sensor, the cooling piece are sticked in the gold
Belong to heat-conducting block lower surface, the radiator is mounted on the radiating surface of the cooling piece, and first temperature sensor is for examining
Know the temperature of the metal heat-conducting block；
Upper heating component comprising heating cover board, heating elements and second temperature sensor, the heating elements are mounted on
On the heating cover board, the second temperature sensor is set for heating the temperature of cover board, the heating cover plate lid described in detection
In the upper surface of sample orifice plate；
In sealer, the temperature of the heating cover board is 100-200 DEG C, and the temperature of the metal heat-conducting block is 0-100 DEG C；
In PCR reaction, the temperature of the heating cover board is 55-105 DEG C, and the temperature of the metal heat-conducting block is 0-100
℃。
Compared to the prior art, this PCR instrument has both sealer function and PCR function to the utility model, has saved laboratory and has matched
It is set to this, reduces laboratory operating procedures；When upper heating assembly high-temperature sealer, lower temperature-controlling component provides low temperature for sample orifice plate and protects
Dis environment effectively prevent top sealer high temperature that sample is caused to be denaturalized；When PCR reacts, under upper heating component can provide slightly above
The temperature of temperature-controlling component PCR reaction, upper heat and lower cold can be effectively prevented sample and volatilize upwards.
Further, the lower surface of the heating cover board is equipped with multiple annular flanges, the annular flange and sample orifice plate
Hole groove location match, the bore of the outer diameter of the annular flange slightly larger than the hole slot upper opening of the sample orifice plate.
The utility model compared to the prior art, it is close can to form a circle between the hole slot collar extension and film of sample orifice plate
Envelope melts trace, improves sealer efficiency and sealing reliability.
Further, protrusion height of the annular flange in heating cover board lower surface is 0.2-1mm.
Using the above preferred scheme, sealer effect had both been improved, it can steady heat transfer when also ensuring that PCR reaction.
Further, the lower temperature-controlling component further includes the first thermal insulation separation heat pad enclosed set on the metal heat-conducting block；Institute
Stating heating component further includes the second thermal insulation separation heat pad enclosed set on the heating cover board.
Using the above preferred scheme, heat insulation effect is improved, heat losses are prevented.
Further, the upper heating component further includes upper bracket, and the upper bracket is equipped with guide post, the heating cover board
It can be moved up and down along guide post, spring is arranged on guide post, the spring setting is between heating cover board and upper bracket.
Using the above preferred scheme, stable pressure can be provided between heating cover board and sample orifice plate, improve sealer
Effect and heat transfer efficiency.
Further, further include main frame, the main frame be equipped with upper and lower translation guide rail, it is described it is upper heating component it is upper
Bracket is mounted on the upper and lower translation guide rail.
It further, further include pedestal, the pedestal is equipped with anterior-posterior translation guide rail, and the lower temperature-controlling component is mounted on institute
It states on anterior-posterior translation guide rail.
It further, further include driving mechanism, the driving mechanism drives the upper heating component vertically translating rails
Mobile, the driving mechanism also drives the lower temperature-controlling component to move along anterior-posterior translation guide rail.
Using the above preferred scheme, the placement that can be convenient sample orifice plate, prevents blanching to hurt, and improves safety.
Further, the radiator of the lower temperature-controlling component includes water-cooled plate, and circulating cooling Jie is connected in the water-cooled plate
Matter.
Using the above preferred scheme, water-cooled plate heat dissipation is more uniformly quick, helps the temperature control essence for improving lower temperature-controlling component
Degree.
Further, the radiator of the lower temperature-controlling component includes radiating fin and radiator fan, and the radiator fan is
The radiating fin provides cooling air-flow.
Using the above preferred scheme, compact-sized, deployment cost is low.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is a kind of structural schematic diagram of embodiment of the utility model；
Fig. 2 is a kind of structural schematic diagram of embodiment of the utility model；
Fig. 3 is a kind of structural schematic diagram of embodiment of the utility model；
Fig. 4 is a kind of structural schematic diagram of embodiment of the utility model.
The title of number and corresponding component represented by letter in figure:
1- metal heat-conducting block；Temperature-controlling component under 2-；21- cooling piece；22- radiator；23- the first thermal insulation separation heat pad；On 3-
Heat component；31- heats cover board；311- annular flange；32- heating elements；33- the second thermal insulation separation heat pad；34- upper bracket；35-
Guide post；36- spring；4- main frame；41- upper and lower translation guide rail；5- pedestal；51- anterior-posterior translation guide rail；6- sample orifice plate.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts
Every other embodiment obtained, fall within the protection scope of the utility model.
As shown in Figure 1, a kind of PCR instrument with sealer function, comprising:
Metal heat-conducting block 1, block are equipped with the hole slot for placing sample orifice plate；
Lower temperature-controlling component 2 comprising cooling piece 21, radiator 22 and the first temperature sensor, cooling piece 21 are sticked in gold
Belong to 1 lower surface of heat-conducting block, radiator 22 is mounted on the radiating surface of cooling piece 21, and first temperature sensor is for detection gold
Belong to the temperature of heat-conducting block 1；
Upper heating component 3 comprising heating cover board 31, heating elements 32 and second temperature sensor, heating elements 32 are pacified
On heating cover board 31, temperature of the second temperature sensor for detection heating cover board 31, the heating lid of cover board 31 is located at
The upper surface of sample orifice plate；
In sealer, the temperature of heating cover board 31 is 100-200 DEG C, and the temperature of metal heat-conducting block 1 is 0-100 DEG C；
In PCR reaction, the temperature of heating cover board 31 is 55-105 DEG C, and the temperature of metal heat-conducting block 1 is 0-100 DEG C.
Beneficial effect by adopting the above technical scheme is: this PCR instrument has both sealer function and PCR function, has saved experiment
Room deployment cost, reduces laboratory operating procedures；When upper heating assembly high-temperature sealer, lower temperature-controlling component provides low for sample orifice plate
Warm Conservation environment effectively prevent top sealer high temperature that sample is caused to be denaturalized；When PCR reacts, upper heating component can provide slightly higher
In the temperature of lower temperature-controlling component PCR reaction, upper heat and lower cold can be effectively prevented sample and volatilize upwards.
As shown in Figure 2,3, in other embodiments of the utility model, the lower surface of heating cover board 31 is equipped with multiple
Annular flange 311, annular flange 311 and the hole groove location of sample orifice plate 6 match, and the outer diameter of annular flange 311 is slightly larger than examination
The bore of the hole slot upper opening of sample orifice plate 6.Beneficial effect by adopting the above technical scheme is: can be outside the hole slot of sample orifice plate
A circle sealing is formed between mouth and film and melts trace, improves sealer efficiency and sealing reliability.
In other embodiments of the utility model, protrusion height of the annular flange 311 in heating cover board lower surface
For 0.2-1mm.Beneficial effect by adopting the above technical scheme is: both having improved sealer effect, can stablize biography when also ensuring that PCR reaction
Heat.
As shown in Figure 1, lower temperature-controlling component 2 further includes enclosing set on metal in other embodiments of the utility model
First thermal insulation separation heat pad 23 of heat-conducting block 1；Upper heating component 3 further includes enclosing the second thermal insulation separation heat pad for being set to heating cover board 31
33.Beneficial effect by adopting the above technical scheme is: improving heat insulation effect, prevents heat losses.
As shown in Figure 1, upper heating component 3 further includes upper bracket 34 in other embodiments of the utility model, on
Bracket 34 is equipped with guide post 35, and heating cover board 31 can be moved up and down along guide post 35, spring 36 is arranged on guide post 35, spring 36 is set
It sets between heating cover board 31 and upper bracket 34.Beneficial effect by adopting the above technical scheme is: for heating cover board and sample aperture
Stable pressure can be provided between plate, improve sealer effect and heat transfer efficiency.
As shown in figure 4, further including main frame 4 in other embodiments of the utility model, main frame 4 is equipped with
The upper bracket 34 of upper and lower translation guide rail 41, upper heating component 3 is mounted on upper and lower translation guide rail 41；It further include pedestal 5, pedestal 5
It is equipped with anterior-posterior translation guide rail 51, lower temperature-controlling component 2 is mounted on anterior-posterior translation guide rail 51；It further include driving mechanism, the drive
Motivation structure drives heating component 3, and vertically translating rails 41 are mobile, and the driving mechanism also drives lower temperature-controlling component 2 along front and back
Translating rails 51 are mobile.Beneficial effect by adopting the above technical scheme is: can be convenient the placement of sample orifice plate, prevents blanching
Wound improves safety.
In other embodiments of the utility model, the radiator 22 of lower temperature-controlling component 2 includes water-cooled plate, the water
Circulating cooling medium is connected in cold plate.Beneficial effect by adopting the above technical scheme is: water-cooled plate heat dissipation is more uniformly quick, has
Help the temperature-controlled precision for improving lower temperature-controlling component.
In other embodiments of the utility model, the radiator 22 of lower temperature-controlling component 2 includes radiating fin and dissipates
Hot-air fan, the radiator fan provide cooling air-flow for the radiating fin.Beneficial effect by adopting the above technical scheme is: knot
Structure is compact, and deployment cost is low.
The above embodiments are only for explaining the technical ideas and features of the present invention, and its object is to allow the common skill in this field
Art personnel can understand the content of the utility model and be implemented, and not limit the protection scope of the present invention,
All equivalent change or modifications according to made by the spirit of the present invention essence, should all cover in the protection scope of the utility model
It is interior.
Claims (10)
1. a kind of PCR instrument with sealer function characterized by comprising
Metal heat-conducting block, block are equipped with the hole slot for placing sample orifice plate；
Lower temperature-controlling component comprising cooling piece, radiator and the first temperature sensor, the cooling piece is sticked leads in the metal
Heat block lower surface, the radiator are mounted on the radiating surface of the cooling piece, and first temperature sensor is used for detection institute
State the temperature of metal heat-conducting block；
Upper heating component comprising heating cover board, heating elements and second temperature sensor, the heating elements are mounted on described
It heats on cover board, the second temperature sensor is located at examination for heating the temperature of cover board, the heating cover plate lid described in detection
The upper surface of sample orifice plate；
In sealer, the temperature of the heating cover board is 100-200 DEG C, and the temperature of the metal heat-conducting block is 0-100 DEG C；
In PCR reaction, the temperature of the heating cover board is 55-105 DEG C, and the temperature of the metal heat-conducting block is 0-100 DEG C.
2. the PCR instrument according to claim 1 with sealer function, which is characterized in that the lower surface of the heating cover board is set
There are multiple annular flanges, the annular flange and the hole groove location of sample orifice plate match, and the outer diameter of the annular flange is bigger
In the bore of the hole slot upper opening of the sample orifice plate.
3. the PCR instrument according to claim 2 with sealer function, which is characterized in that the annular flange is in heating cover board
The protrusion height of lower surface is 0.2-1mm.
4. the PCR instrument according to claim 3 with sealer function, which is characterized in that the lower temperature-controlling component further includes enclosing
Set on the first thermal insulation separation heat pad of the metal heat-conducting block；The upper heating component further includes enclosing the set on the heating cover board
Two thermal insulation separation heat pads.
5. the PCR instrument according to claim 1 with sealer function, which is characterized in that on the upper heating component further includes
Bracket, the upper bracket are equipped with guide post, and the heating cover board can be moved up and down along guide post, and spring is arranged on guide post, described
Spring setting is between heating cover board and upper bracket.
6. the PCR instrument according to claim 5 with sealer function, which is characterized in that it further include main frame, the main frame
It is equipped with upper and lower translation guide rail, the upper bracket of the upper heating component is mounted on the upper and lower translation guide rail.
7. the PCR instrument according to claim 6 with sealer function, which is characterized in that further include pedestal, set on the pedestal
There is anterior-posterior translation guide rail, the lower temperature-controlling component is mounted on the anterior-posterior translation guide rail.
8. the PCR instrument according to claim 7 with sealer function, which is characterized in that it further include driving mechanism, the driving
Mechanism drives the upper heating component, and vertically translating rails are mobile, and the driving mechanism also drives the lower temperature-controlling component before
Translating rails are mobile afterwards.
9. the PCR instrument according to claim 1 with sealer function, which is characterized in that the radiator of the lower temperature-controlling component
Including water-cooled plate, circulating cooling medium is connected in the water-cooled plate.
10. the PCR instrument according to claim 1 with sealer function, which is characterized in that the radiator of the lower temperature-controlling component
Including radiating fin and radiator fan, the radiator fan provides cooling air-flow for the radiating fin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821823484.XU CN209178400U (en) | 2018-11-07 | 2018-11-07 | A kind of PCR instrument with sealer function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821823484.XU CN209178400U (en) | 2018-11-07 | 2018-11-07 | A kind of PCR instrument with sealer function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209178400U true CN209178400U (en) | 2019-07-30 |
Family
ID=67363406
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821823484.XU Active CN209178400U (en) | 2018-11-07 | 2018-11-07 | A kind of PCR instrument with sealer function |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110564610A (en) * | 2019-10-15 | 2019-12-13 | 杭州比芯诊断技术有限公司 | Double-temperature-zone PCR amplification device |
| CN111229350A (en) * | 2019-09-27 | 2020-06-05 | 杭州柏恒科技有限公司 | Liquid cooling automatic PCR equipment |
| CN112063496A (en) * | 2020-11-11 | 2020-12-11 | 上海鹍远生物技术有限公司 | Closed PCR (polymerase chain reaction) instrument suitable for full-automatic pipetting workstation and application |
| CN112963982A (en) * | 2021-04-15 | 2021-06-15 | 上海申彦生物科技有限公司 | Heating and refrigerating module for nucleic acid extractor |
| CN115011462A (en) * | 2022-06-30 | 2022-09-06 | 苏州百源基因技术有限公司 | Refrigerating device suitable for PCR instrument |
| CN115851149A (en) * | 2022-12-21 | 2023-03-28 | 莫纳(武汉)生物科技有限公司 | Sealing plate film and PCR (polymerase chain reaction) hot cap sealing method |
-
2018
- 2018-11-07 CN CN201821823484.XU patent/CN209178400U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111229350A (en) * | 2019-09-27 | 2020-06-05 | 杭州柏恒科技有限公司 | Liquid cooling automatic PCR equipment |
| CN110564610A (en) * | 2019-10-15 | 2019-12-13 | 杭州比芯诊断技术有限公司 | Double-temperature-zone PCR amplification device |
| CN112063496A (en) * | 2020-11-11 | 2020-12-11 | 上海鹍远生物技术有限公司 | Closed PCR (polymerase chain reaction) instrument suitable for full-automatic pipetting workstation and application |
| CN112963982A (en) * | 2021-04-15 | 2021-06-15 | 上海申彦生物科技有限公司 | Heating and refrigerating module for nucleic acid extractor |
| CN115011462A (en) * | 2022-06-30 | 2022-09-06 | 苏州百源基因技术有限公司 | Refrigerating device suitable for PCR instrument |
| CN115851149A (en) * | 2022-12-21 | 2023-03-28 | 莫纳(武汉)生物科技有限公司 | Sealing plate film and PCR (polymerase chain reaction) hot cap sealing method |
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