CN210261762U

CN210261762U - Temperature control device for PCR amplification device

Info

Publication number: CN210261762U
Application number: CN201921071479.2U
Authority: CN
Inventors: 张志峰; 王晓飞
Original assignee: Chengdu Wanzhong One Core Biotechnology Co ltd
Current assignee: Chengdu Wanzhong One Core Biotechnology Co ltd
Priority date: 2019-07-09
Filing date: 2019-07-09
Publication date: 2020-04-07
Anticipated expiration: 2029-07-09

Abstract

The utility model discloses a temperature control device for PCR amplification device, including the thermocycler, the thermocycler includes base and movable mounting and is in the upper cover at base top. The upper end of base is equipped with the chamber of placing that is used for holding micropore array chip board, the below of placing the chamber is equipped with and is used for adjusting place the temperature control subassembly of chamber temperature. The temperature control assembly comprises a heat conduction block which is vertically arranged and is abutted against the bottom of the placing cavity. The upper end of the heat conduction block is provided with a temperature sensor for detecting the temperature of the placing cavity, and the lower end of the heat conduction block is provided with a heater and a radiator which are placed up and down. The utility model discloses can effective control nucleic acid molecule's amplification temperature, guarantee that being heated of micropore array chip board is even, improve the uniformity of nucleic acid molecule amplification efficiency.

Description

Temperature control device for PCR amplification device

Technical Field

The utility model relates to a PCR detects the field, especially relates to a temperature control device for PCR amplification device.

Background

Modern biological research, particularly medical research, often involves the need for quantitative analysis of nucleic acids. For example, detection of certain free DNA and its amount in blood can guide the clinical diagnosis of certain cancers and monitor the therapeutic effect of cancer. In the conventional quantification, the amount of a target nucleic acid is determined by selecting a transcript of a gene whose expression is stable as a reference, and performing relative quantification mainly by fluorescent quantitative PCR. However, this method is susceptible to interference from non-target nucleic acid molecules (background noise), and is only suitable for qualitative or low-precision detection, and cannot satisfy detection of target nucleic acid molecules with a particularly rare content.

Digital PCR (dpcr) is a method of counting the number of molecules amplified by "the presence or absence of an end-point signal" by extracting weak amplification signals from background noise through large-scale parallel PCR amplification. At present, the PCR amplification device is used for detecting target nucleic acid molecules, so that the absolute quantitative precision and the dynamic range of DNA can be very high, and the cost and the operation time are greatly reduced. At present, a PCR amplification device is used for discretizing nucleic acid molecules on a micropore array chip board to realize digital amplification, but the existing PCR amplification device is lack of an effective temperature control device and cannot control the temperature change in the sample amplification process, and the temperature uniformity of the nucleic acid molecules at different positions is poor, so that the amplification efficiency is inconsistent.

SUMMERY OF THE UTILITY MODEL

In order to overcome the above disadvantages, an object of the present invention is to provide a temperature control device for a PCR amplification device, which can effectively control the amplification temperature of nucleic acid molecules, ensure the uniformity of heating of the micro-porous array chip plate, and improve the uniformity of the amplification efficiency of nucleic acid molecules.

In order to achieve the above purpose, the utility model discloses a technical scheme is: a temperature control device for a PCR amplification device comprises a thermal cycler, wherein the thermal cycler comprises a base and an upper cover movably mounted at the top of the base. The upper end of base is equipped with the chamber of placing that is used for holding micropore array chip board, the below of placing the chamber is equipped with and is used for adjusting place the temperature control subassembly of chamber temperature. The temperature control assembly comprises a heat conduction block which is vertically arranged and is abutted against the bottom of the placing cavity. The upper end of the heat conduction block is provided with a temperature sensor for detecting the temperature of the placing cavity, and the lower end of the heat conduction block is provided with a heater and a radiator which are placed up and down.

Further, the radiator comprises a radiating fin positioned at the bottom of the heater, the lower end of the radiating fin is fixedly connected with a centrifugal fan, and one side of the radiating fin is fixedly connected with a flow guide cover used for guiding airflow.

Further, the temperature control assembly further comprises a PCB arranged on the outer side of the heat conducting block, and the PCB is respectively and electrically connected with the temperature sensor, the heater and the radiator through a control circuit. The temperature sensed by the temperature sensor can be timely fed back to the PCB through the control circuit, and the PCB controls the starting of the heater or the radiator according to the actual temperature requirement of the PCR amplification reaction so as to ensure that the required reaction temperature is reached on the micropore array chip board.

Further, a pair of limiting plates which are symmetrically arranged is fixedly connected to the base, and the heat conducting block is limited and fixed between the limiting plates. The position of the heat conducting block is limited by the limiting plate, so that the heat conducting block is fixed on the base.

Further, the temperature control assembly further comprises a positioning plate fixedly connected to the upper end of the radiator, and a through groove communicated up and down is formed in the positioning plate. The through groove comprises an upper caulking groove used for being embedded into the lower end part of the heat conducting block and a lower caulking groove used for being embedded into the heater. The heater is positioned in the lower embedded groove, the upper end of the heater is connected with the heat conducting block, and the lower end of the heater is connected with the radiator. The fixed connection of the heat conducting block, the heater and the radiator is realized through the positioning plate.

Further, the heater is a semiconductor heater or a thermal resistance heater.

Further, the longitudinal section of the heat conducting block is of an inverted T-shaped structure, and the bottom of the heat conducting block is completely attached to the heater. The contact area of the heat conducting block and the heater is increased through the heat conducting block with the inverted T-shaped structure, and the heat conducting efficiency is improved.

The beneficial effects of the utility model reside in that: the upper cover movably arranged on the base realizes the opening and closing of the placing cavity, is convenient for taking and placing the micropore array chip plate, and enables the placing cavity to form a closed cavity during working; the temperature of the placing cavity is adjusted by the temperature control component to reach the required amplification temperature; the temperature of the placing cavity can be monitored in real time through the temperature sensor, when the monitored temperature is too low, the temperature is conducted to the bottom of the placing cavity through the heater and the heat conducting block, so that the placing cavity is uniformly heated from the bottom upwards gradually, and the nucleic acid molecules on the micropore array chip plate are ensured to be heated synchronously; when the temperature of monitoring is too high, the heat that will place the chamber through the radiator heat dissipation and through the heat conduction piece is released from the bottom gradually for place the even cooling of chamber, guarantee the synchronous cooling of nucleic acid molecule on the micropore array chip board. The utility model discloses a temperature control device can effectively control the amplification temperature of nucleic acid molecule, guarantees being heated of micropore array chip board even, and then improves the uniformity of nucleic acid molecule amplification efficiency.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention;

fig. 2 is a sectional view taken along the line a-a in fig. 1.

In the figure:

1-a microwell array chip plate; 2-a thermal cycler; 21-upper cover; 22-a heat conducting block; 23-a heater; 24-a heat sink; 241-a heat sink; 242-centrifugal fan; 243-air guide sleeve; 25-a PCB board; 26-a limiting plate; 27-a positioning plate; 28-placing the cavity; 29-temperature sensor.

Detailed Description

The following detailed description of the preferred embodiments of the present invention will be provided in conjunction with the accompanying drawings, so as to enable those skilled in the art to more easily understand the advantages and features of the present invention, and thereby define the scope of the invention more clearly and clearly.

Examples

Referring to fig. 1-2, the temperature control device for a PCR amplification apparatus of the present invention comprises a thermal cycler 2, wherein the thermal cycler 2 comprises a base and an upper cover 21 movably mounted on the top of the base. The upper end of base 21 is equipped with the chamber 28 of placing that is used for holding micropore array chip board 1, the below of placing chamber 28 is equipped with and is used for adjusting place the temperature control component of chamber 28 temperature. The upper cover 21 is provided with a transparent viewing port located right above the placing cavity 28.

The temperature control assembly comprises a heat conducting block 22 which is vertically arranged and has an inverted T-shaped structure in longitudinal section, the heat conducting block 22 is made of high heat conducting material, and the upper end of the heat conducting block abuts against the bottom of the placing cavity 28. A pair of limiting plates 26 which are symmetrically arranged is fixedly connected to the base, and the heat conducting block 22 is limited and fixed between the limiting plates 26.

The upper end of the heat conducting block 22 is provided with a groove, and the lower end is provided with a heater 23 for heating. The bottom of the heat conduction block 22 is completely attached to the heater 23. A temperature sensor 29 is arranged in the groove, and the upper end of the temperature sensor 29 is abutted against the bottom of the placing cavity 28 to detect the temperature of the placing cavity 28. The bottom of the heater 23 is provided with a heat sink 24. The outer side of the heat conducting block 22 is also provided with a PCB 25, and the PCB 25 is respectively and electrically connected with the temperature sensor 29, the heater 23 and the radiator 24 through a control circuit.

The temperature control assembly further comprises a positioning plate 27 fixedly connected to the upper end of the radiator 24, and a through groove communicated up and down is formed in the positioning plate 27; the through grooves comprise an upper embedded groove used for embedding the lower end part of the heat conducting block 22 and a lower embedded groove used for embedding the heater 23; the heater 23 is located in the lower caulking groove, the upper end of the heater is attached to the bottom of the heat conducting block 22, and the lower end of the heater is attached to the radiator 24. The positioning plate 27 realizes the fixed connection of the heat conduction block 22, the heater 23 and the radiator 24.

The heater 23 is a semiconductor heater or a thermal resistance heater, and includes a housing, a heat conducting cavity for placing a heat conducting medium is provided in the housing, and a controller electrically connected to the PCB 25 is provided at one side of the housing. The lower extreme in heat conduction chamber is equipped with the heating element who is used for heating heat conduction chamber. The heat conduction device is characterized in that a partition plate is arranged between the heat conduction cavity and the heating assembly, a plurality of protruding parts extending into the heat conduction cavity are arranged on the partition plate side by side, a channel for heat conduction medium circulation is defined between the adjacent protruding parts and between the protruding part located on the outermost side and the side wall of the heat conduction cavity, and partition strips located in the channel are vertically arranged on the protruding parts and the side wall of the heat conduction cavity. The flow track of the heat-conducting medium can be changed through the arrangement of the protruding portions and the partition bars, the turbulence of the heat-conducting medium is increased, the heat exchange efficiency is further improved, and the heating is faster.

The heat sink 24 includes a heat sink 241 located at the bottom of the heater 23, a centrifugal fan 242 is fixed to the lower end of the heat sink 241, and a flow guiding cover 243 for guiding airflow is fixed to one side of the heat sink.

The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, so as not to limit the protection scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be covered in the protection scope of the present invention.

Claims

1. A temperature control apparatus for a PCR amplification apparatus, characterized in that: the device comprises a thermal circulator (2), wherein the thermal circulator (2) comprises a base and an upper cover (21) movably mounted on the top of the base; the upper end of the base is provided with a placing cavity (28) for accommodating the micropore array chip plate (1), and a temperature control assembly for adjusting the temperature of the placing cavity (28) is arranged below the placing cavity (28); the temperature control assembly comprises a heat conduction block (22) which is vertically arranged and is abutted against the bottom of the arrangement cavity (28); the upper end of the heat conducting block (22) is provided with a temperature sensor (29) for detecting the temperature of the placing cavity (28), and the lower end of the heat conducting block (22) is provided with a heater (23) and a radiator (24) which are placed up and down.

2. The temperature control apparatus for a PCR amplification apparatus according to claim 1, wherein: the radiator (24) comprises radiating fins (241) positioned at the bottom of the heater (23), the lower ends of the radiating fins (241) are fixedly connected with centrifugal fans (242), and one side of each radiating fin is fixedly connected with a flow guide cover (243) used for guiding airflow.

3. The temperature control apparatus for a PCR amplification apparatus according to claim 1, wherein: the temperature control assembly further comprises a PCB (25) arranged on the outer side of the heat conducting block (22), and the PCB (25) is respectively and electrically connected with the temperature sensor (29), the heater (23) and the radiator (24) through a control circuit.

4. The temperature control apparatus for a PCR amplification apparatus according to claim 1, wherein: the base is further fixedly connected with a pair of limiting plates (26) which are symmetrically arranged, and the heat conducting block (22) is limited and fixed between the limiting plates (26).

5. The temperature control apparatus for a PCR amplification apparatus according to claim 1, wherein: the temperature control assembly further comprises a positioning plate (27) fixedly connected to the upper end of the radiator (24), and a through groove communicated up and down is formed in the positioning plate (27); the through grooves comprise upper embedded grooves used for embedding the lower end parts of the heat conducting blocks (22) and lower embedded grooves used for embedding the heaters (23); the heater (23) is positioned in the lower embedded groove, the upper end of the heater is connected with the heat conducting block (22), and the lower end of the heater is connected with the radiator (24).

6. The temperature control apparatus for a PCR amplification apparatus according to any one of claims 1 to 5, wherein: the heater (23) is a semiconductor heater or a thermal resistance heater.

7. The temperature control apparatus for a PCR amplification apparatus according to claim 1, wherein: the longitudinal section of the heat conducting block (22) is of an inverted T-shaped structure, and the bottom of the heat conducting block is completely attached to the heater (23).