CN113832023B - PCR micro-fluidic temperature control device - Google Patents
PCR micro-fluidic temperature control device Download PDFInfo
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- CN113832023B CN113832023B CN202111258797.1A CN202111258797A CN113832023B CN 113832023 B CN113832023 B CN 113832023B CN 202111258797 A CN202111258797 A CN 202111258797A CN 113832023 B CN113832023 B CN 113832023B
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a PCR (polymerase chain reaction) microfluidic temperature control device which comprises a machine body, a heating assembly and a lifting assembly, wherein the machine body is provided with an installation space, the heating assembly comprises a heating table and a pressing plate, the pressing plate and the heating table are movably arranged in the installation space and are oppositely arranged, the heating table is used for containing and heating a reagent card, and the lifting assembly is connected with the heating table and/or the pressing plate so as to drive the heating table and/or the pressing plate to enable the heating table and the pressing plate to be close to or far away from each other so as to compress or release the reagent card. The invention aims to provide the PCR micro-fluidic temperature control device with low heat loss, high temperature rising and reducing speed and simple structure, which effectively reduces the heat loss, simultaneously realizes the close degree of the amplification area of the reagent disk and the heat conducting plate, and improves the temperature rising and reducing speed of the reagent disk.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a PCR (polymerase chain reaction) microfluidic temperature control device.
Background
The polymerase chain reaction is PCR (Polymerase Chain Reaction) for short, and the PCR is a method for synthesizing specific DNA fragments by in vitro enzymatic hydrolysis, and the method relates to the application of a microfluidic device, namely the microfluidic device enables reaction liquid reagents to be subjected to periodical and cyclic treatment through steps of heating at different temperatures and the like at different stages, so that target DNA can be rapidly amplified. The PCR has the characteristics of strong specificity, high sensitivity, simple and convenient operation, time saving and the like; it can be used for basic research such as gene separation, cloning and nucleic acid sequence analysis, and can also be used for diagnosing diseases.
Microfluidic refers to manipulation of liquids on a sub-millimeter scale, typically in the scale range of a few microns to hundreds of microns. The microfluidic system integrates basic operation units related to the biological and chemical fields, namely the whole assay operation including sampling, dilution, reaction, separation, detection and the like, on a small Chip, so that the microfluidic system is also called a lab-on-a-Chip (Labon-a-Chip), and can not only simplify operation steps, shorten detection time, save reagent consumption, but also improve detection accuracy and sensitivity.
In order to meet clinical requirements, sample detection time is shortened, and the temperature rise and fall rate, temperature consistency and heat transfer efficiency of the system are required to be controlled. In the related art, the heating block and the reagent disk are generally arranged into relative rotating structures, so that sliding friction is generated between the heating block and the reagent disk, the surface of the disk is worn, the accuracy of optical detection is affected, meanwhile, air heat transfer is adopted, heating is uneven, the temperatures of different areas cannot be agreed quickly, and temperature control is difficult to guarantee.
Disclosure of Invention
The invention mainly aims to provide a PCR micro-fluidic temperature control device, and aims to provide the PCR micro-fluidic temperature control device with low heat loss, high temperature rise and fall speed and simple structure.
In order to achieve the above object, the present invention provides a PCR microfluidic temperature control device, including:
the machine body is provided with an installation space;
The heating assembly comprises a heating table and a pressing plate, the pressing plate and the heating table are movably arranged in the installation space and are oppositely arranged, and the heating table is used for containing and heating the reagent card; and
And the lifting assembly is connected with the heating table and/or the pressing plate so as to drive the heating table and/or the pressing plate to move, so that the heating table and the pressing plate are close to or far away from each other, and the reagent card is pressed or released.
In one embodiment, the heating table is provided with a constant temperature area and a variable temperature area which are arranged at intervals;
the pressing plate comprises a pressing part and foam, the pressing part and the temperature changing area are movably arranged in the installation space, the pressing part is opposite to the temperature changing area, and the foam is arranged on one side of the pressing part facing the temperature changing area.
In one embodiment, the heating station comprises:
the base is movably arranged in the installation space;
the temperature changing block is arranged on the base and corresponds to the pressing plate so as to form the temperature changing area; and
The constant temperature block is arranged on the base and is spaced from the variable temperature block to form the constant temperature zone.
In one embodiment, the temperature change block comprises:
The first heat insulation block is arranged on the base and corresponds to the pressing plate, and a first mounting groove is formed in one side, facing away from the base, of the first heat insulation block;
The refrigerator is arranged in the first mounting groove; and
The first heat conduction plate is arranged on the first heat insulation block so as to cover the notch of the first mounting groove.
In an embodiment, the temperature changing block further includes a heat sink and a heat dissipating fan, the heat sink is disposed on a side of the first heat insulating block facing away from the first heat conducting plate, the base is provided with a via hole corresponding to the heat sink, and the heat dissipating fan is disposed on a side of the base facing away from the temperature changing block and is disposed corresponding to the heat sink;
and/or, the temperature changing block further comprises a first protection switch and a first temperature sensor, the first protection switch is electrically connected with the first temperature sensor, a first accommodating groove and a second accommodating groove are formed in the first heat insulating block at intervals, the first protection switch is arranged in the first accommodating groove, and the first temperature sensor is arranged in the second accommodating groove and is in butt joint with the first heat conducting plate;
and/or the refrigerator comprises a plurality of first heat insulation blocks, wherein a plurality of first mounting grooves are formed in the first heat insulation blocks, and each refrigerator is arranged in one first mounting groove;
and/or heat conduction silicone grease is filled between the first heat conduction plate and the refrigerator.
In one embodiment, the thermostatic block comprises:
The second heat insulation block is arranged on the base and is positioned at one end of the base far away from the temperature changing block, and a second mounting groove is formed in one side of the second heat insulation block, which is away from the base;
The heating film is arranged in the second mounting groove; and
The second heat conduction plate is arranged on the second heat insulation block so as to cover the notch of the second mounting groove.
In an embodiment, the constant temperature block further includes a second protection switch and a second temperature sensor, the second protection switch is electrically connected with the second temperature sensor, the second heat insulation block is provided with a first accommodating groove and a second accommodating groove at intervals, the second protection switch is arranged in the first accommodating groove, and the second temperature sensor is arranged in the second accommodating groove and is abutted to the second heat conducting plate;
And/or heat conduction silicone grease is filled between the second heat conduction plate and the heating film.
In an embodiment, the machine body includes a first fixing plate and a second fixing plate, where the first fixing plate and the second fixing plate are opposite and spaced apart to form the installation space;
the pressing plate is in sliding connection with the first fixing plate, and the heating table is in sliding connection with the second fixing plate.
In one embodiment, the lifting assembly comprises:
the output shaft of the driving piece is connected with the heating table;
The two synchronizing wheels are arranged on the first fixed plate at intervals;
The synchronous belt is sleeved on the two synchronous wheels, and the heating table and the pressing plate are connected with the synchronous belt;
When the driving piece drives the heating platform to move, the heating platform drives the synchronous belt to move so that the heating platform and the pressing plate are close to or far away from each other.
In one embodiment, one of the pressing plate and the first fixing plate is provided with a first sliding table, and the other one of the pressing plate and the first fixing plate is provided with a first sliding groove which is in sliding fit with the first sliding table;
And/or, one of the heating table and the second fixing plate is provided with a second sliding table, and the other one of the heating table and the second fixing plate is provided with a second sliding groove which is in sliding fit with the second sliding table;
And/or, one end of the heating table far away from the pressing plate is provided with a connecting table, the connecting table comprises a sliding part and a connecting part, the sliding part is in sliding connection with the second fixing plate, and the connecting part is connected with an output shaft of the driving piece;
And/or, the hold-in range has first band section and second band section, first band section with the second band section is located two the opposite sides of synchronizing wheel line, the clamp plate is equipped with first connecting seat, first connecting seat with first band section is connected, the heating platform is adjacent the one end of clamp plate is equipped with the second connecting seat, the second connecting seat with the second band section is connected.
According to the PCR microfluidic temperature control device, the heating assembly is arranged in the installation space of the machine body, so that the pressing plate of the heating assembly and the heating table are movably arranged in the installation space of the machine body and are oppositely arranged, the heating table and/or the pressing plate are/is driven to move by utilizing the lifting assembly to be connected with the heating table and/or the pressing plate, the heating table and the pressing plate are/is driven to move to mutually approach, the reagent card on the heating table is pressed by utilizing the pressing plate, so that the air flow on the upper surface of the amplification area of the reagent card can be reduced, the heat loss is effectively reduced, and meanwhile, the reagent card is mutually pressed by the heating table and the pressing plate, so that the sticking degree of the amplification area of the reagent card and the heat conducting plate of the heating table is realized, and the lifting temperature rate of the reagent card is improved. When the operation is finished, the lifting assembly is used for driving the heating table and/or the pressing plate to move, so that the heating table and the pressing plate are away from each other, and the reagent card is released.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a PCR microfluidic temperature control device according to an embodiment of the present invention;
FIG. 2 is a schematic view of a heating stage according to an embodiment of the present invention;
FIG. 3 is a schematic view of a portion of a heating stage according to an embodiment of the present invention;
FIG. 4 is a schematic view of a thermostatic block according to an embodiment of the present invention;
FIG. 5 is a schematic view of a portion of a thermostatic block according to an embodiment of the present invention;
FIG. 6 is a schematic diagram of a temperature changing block according to an embodiment of the present invention;
fig. 7 is a schematic view of a part of a temperature changing block according to an embodiment of the present invention.
Reference numerals illustrate:
| Reference numerals | Name of the name | Reference numerals | Name of the name |
| 100 | PCR micro-fluidic temperature control device | 2311 | Second mounting groove |
| 1 | Body of machine | 2312 | First accommodating groove |
| 11 | Installation space | 2313 | Second accommodating groove |
| 12 | First fixing plate | 232 | Heating film |
| 13 | Second fixing plate | 233 | Second heat conducting plate |
| 14 | First slipway | 234 | Second protective switch |
| 15 | Second sliding table | 235 | Second temperature sensor |
| 2 | Heating table | 24 | Connecting table |
| 21 | Base seat | 241 | Sliding part |
| 22 | Temperature changing block | 242 | Connecting part |
| 221 | First heat insulation block | 243 | Second chute |
| 2211 | First mounting groove | 25 | Second connecting seat |
| 2212 | First accommodating groove | 3 | Pressing plate |
| 2213 | Second accommodating groove | 31 | Pressing part |
| 222 | Refrigerating device | 32 | Foam cotton |
| 223 | First heat conducting plate | 33 | First connecting seat |
| 224 | Heat sink | 4 | Lifting assembly |
| 225 | First protection switch | 41 | Driving piece |
| 226 | First temperature sensor | 42 | Synchronous wheel |
| 23 | Constant temperature block | 43 | Synchronous belt |
| 231 | Second heat insulation block |
The achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present invention are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.
Meanwhile, the meaning of "and/or" and/or "appearing throughout the text is to include three schemes, taking" a and/or B "as an example, including a scheme, or B scheme, or a scheme that a and B satisfy simultaneously.
Furthermore, descriptions such as those referred to as "first," "second," and the like, are provided for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implying an order of magnitude of the indicated technical features in the present disclosure. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present invention.
The polymerase chain reaction is PCR (Polymerase Chain Reaction) for short, and the PCR is a method for synthesizing specific DNA fragments by in vitro enzymatic hydrolysis, and the method relates to the application of a microfluidic device, namely the microfluidic device enables reaction liquid reagents to be subjected to periodical and cyclic treatment through steps of heating at different temperatures and the like at different stages, so that target DNA can be rapidly amplified. The PCR has the characteristics of strong specificity, high sensitivity, simple and convenient operation, time saving and the like; it can be used for basic research such as gene separation, cloning and nucleic acid sequence analysis, and can also be used for diagnosing diseases.
Microfluidic refers to manipulation of liquids on a sub-millimeter scale, typically in the scale range of a few microns to hundreds of microns. The microfluidic system integrates basic operation units related to the biological and chemical fields, namely the whole assay operation including sampling, dilution, reaction, separation, detection and the like, on a small Chip, so that the microfluidic system is also called a lab-on-a-Chip (Labon-a-Chip), and can not only simplify operation steps, shorten detection time, save reagent consumption, but also improve detection accuracy and sensitivity.
In order to meet clinical requirements, sample detection time is shortened, and the temperature rise and fall rate, temperature consistency and heat transfer efficiency of the system are required to be controlled. In the related art, the heating block and the reagent disk are generally arranged into relative rotating structures, so that sliding friction is generated between the heating block and the reagent disk, the surface of the disk is worn, the accuracy of optical detection is affected, meanwhile, air heat transfer is adopted, heating is uneven, the temperatures of different areas cannot be agreed quickly, and temperature control is difficult to guarantee.
Based on the above concepts and problems, the present invention proposes a PCR microfluidic temperature control device 100. It will be appreciated that the PCR microfluidic temperature control device 100 is used to perform assay operations on samples on a reagent card.
Referring to fig. 1 to 3 in combination, in the embodiment of the invention, the PCR microfluidic temperature control device 100 includes a machine body 1, a heating assembly and a lifting assembly 4, wherein the machine body 1 is provided with an installation space 11, the heating assembly includes a heating table 2 and a pressing plate 3, the pressing plate 3 and the heating table 2 are movably disposed in the installation space 11 and are arranged in opposite directions, the heating table 2 is used for holding and heating a reagent card, the lifting assembly 4 is connected with the heating table 2 and/or the pressing plate 3 to drive the heating table 2 and/or the pressing plate 3 to move, so that the heating table 2 and the pressing plate 3 are close to or far from each other to compress or release the reagent card.
In this embodiment, the machine body 1 is used for installing, fixing or supporting the heating assembly, the lifting assembly 4 and other components of the PCR micro-fluidic temperature control device 100, that is, the machine body 1 provides an installation basis for the heating assembly, the lifting assembly 4 and other components of the PCR micro-fluidic temperature control device 100. It should be understood that the machine body 1 may be a mounting rack, a mounting shell, a frame, a machine table, a machine body, a mounting table or a mounting plate, which is not limited herein. Of course, in order to make the heating assembly, the lifting assembly 4, and other components of the PCR microfluidic temperature control device 100 cooperate with each other, the machine body 1 may be further provided with a step structure or a structure such as a gantry, which is not limited herein.
It can be understood that the heating assembly is arranged into a two-part structure of the heating table 2 and the pressing plate 3, so that the reagent card is contained and heated by the heating table 2, and the reagent card can be pressed by the pressing plate 3, so that the air flow on the upper surface of the amplification area of the reagent card is reduced, and the heat loss of the reagent card is effectively reduced; meanwhile, the reagent card is mutually pressed by the heating table 2 and the pressing plate 3, so that the adhesion degree of the reagent card amplification area and the heat conducting plate of the heating table 2 is realized, and the temperature rising and falling speed of the reagent card is improved.
In this embodiment, the lifting assembly 4 is arranged, so that the lifting assembly 4 is connected with the heating table 2 and/or the pressing plate 3 to drive the heating table 2 and/or the pressing plate 3 to move, thereby realizing that the heating table 2 and the pressing plate 3 are close to each other to press the reagent card, or realizing that the heating table 2 and the pressing plate 3 are far away from each other to release the reagent card. It will be appreciated that the lifting assembly 4 may be a driving cylinder, a driving motor, a driving wheel, a synchronous belt, and the like, which are not limited herein.
It will be appreciated that the lifting assembly 4 may comprise one or more lifting assemblies, and that the lifting assembly 4 may be coupled only to the heating table 2 to drive the heating table 2 to move; or the lifting assembly 4 can be connected with the pressing plate 3 only to drive the pressing plate 3 to move; or the lifting assembly 4 is connected with the heating table 2 and the pressing plate 3 to drive the heating table 2 and the pressing plate 3 to move.
According to the PCR microfluidic temperature control device 100, the heating component is arranged in the installation space 11 of the machine body 1, so that the pressing plate 3 and the heating table 2 of the heating component are movably arranged in the installation space 11 of the machine body 1 and are oppositely arranged, the heating table 2 and/or the pressing plate 3 are connected by the lifting component 4 so as to drive the heating table 2 and/or the pressing plate 3 to move, the heating table 2 and the pressing plate 3 are mutually close, the reagent card on the heating table 2 is pressed by the pressing plate 3, the air flow on the upper surface of the reagent card amplification area can be reduced, the heat loss is effectively reduced, and meanwhile, the reagent card is mutually pressed by the heating table 2 and the pressing plate 3, so that the adhesion degree of the reagent card amplification area and the heat conducting plate of the heating table 2 is realized, and the temperature rising and falling speed of the reagent card is improved. When the operation is finished, the heating table 2 and/or the pressing plate 3 are driven to move by the lifting assembly 4, so that the heating table 2 and the pressing plate 3 are away from each other to release the reagent card.
In one embodiment, as shown in fig. 1, the heating table 2 has a constant temperature area and a variable temperature area which are arranged at intervals; the pressing plate 3 comprises a pressing part 31 and foam 32 which are movably arranged in the installation space 11, the pressing part 31 is opposite to the temperature changing area, and the foam 32 is arranged on one side of the pressing part 31 facing the temperature changing area.
It can be appreciated that by providing a constant temperature zone and a variable temperature zone on the heating table 2, a constant temperature heat source is provided for the extraction process of the sample on the reagent card by using the constant temperature zone, and temperature circulation is provided for three stages of denaturation, annealing and extension in the amplification of the sample on the reagent card by using the variable temperature zone.
In this embodiment, through setting up clamp plate 3 as compressing tightly portion 31 and bubble cotton 32 for compressing tightly portion 31 can compress tightly the reagent card, utilizes bubble cotton 32 to realize keeping warm to the reagent card, in order to effectively reduce the heat loss. It is to be understood that the pressing portion 31 may be a plate structure, and the pressing portion 31 may be made of plastic, wood, metal, or the like, which is not limited herein.
In an embodiment, the heating stage 2 includes a base 21, a temperature changing block 22 and a constant temperature block 23, wherein the base 21 is movably disposed in the installation space 11, the temperature changing block 22 is disposed on the base 21 and corresponds to the pressing plate 3 to form a temperature changing area, and the constant temperature block 23 is disposed on the base 21 and is spaced from the temperature changing block 22 to form a constant temperature area.
It can be appreciated that by arranging the temperature changing block 22 and the constant temperature block 23 on the base 21, the temperature changing block 22 corresponds to the pressing plate 3, so that the pressing plate 3 can be utilized to press the reagent card on the temperature changing block 22, and the sample of the reagent card can be amplified smoothly. In this embodiment, the temperature change block 22 can provide a temperature change in the range of 40℃to 100℃to suit the different stage requirements of sample amplification of the reagent card. The thermostat block 23 may provide a temperature environment of 105 c.
In this embodiment, as shown in fig. 1 to 7, the temperature changing block 22 includes a first heat insulating block 221, a refrigerator 222 and a first heat conducting plate 223, wherein the first heat insulating block 221 is disposed on the base 21 and is disposed corresponding to the pressing plate 3, a first mounting groove 2211 is disposed on a side of the first heat insulating block 221 facing away from the base 21, the refrigerator 222 is disposed in the first mounting groove 2211, and the first heat conducting plate 223 is disposed on the first heat insulating block 221 and abuts against the refrigerator 222 to cover a notch of the first mounting groove 2211.
In the present embodiment, by disposing the refrigerator 222 in the first mounting groove 2211 of the first heat insulating block 221, on one hand, the heat insulation of the base 21 is achieved by the first heat insulating block 221, and the limitation of the refrigerator 222 is achieved, on the other hand, the heat loss of the refrigerator 222 is effectively reduced. It can be appreciated that by providing the first heat conductive plate 223, it is convenient to achieve rapid heat transfer using the first heat conductive plate 223, and at the same time, to achieve temperature uniformity of the reagent card using the first heat conductive plate 223, so as to improve accuracy.
Optionally, a heat-conducting silicone grease is filled between the first heat-conducting plate 223 and the refrigerator 222, so that the heat-conducting efficiency can be further improved. In this embodiment, the refrigerator 222 includes a plurality of first heat insulation blocks 221 having a plurality of first mounting slots 2211, and each refrigerator 222 is disposed in a first mounting slot 2211. Optionally, the refrigerator 222 is a TEC (semiconductor refrigerator Thermo Electric Cooler). Optionally, the first heat conducting plate 223 is an aluminum plate, and the material of the first heat insulating block 221 is an epoxy resin material.
In an embodiment, as shown in fig. 1, 6 and 7, the temperature changing block 22 further includes a heat dissipating fin 224 and a heat dissipating fan, the heat dissipating fin 224 is disposed on a side of the first heat insulating block 221 facing away from the first heat conducting plate 223, the base 21 is provided with a via hole corresponding to the heat dissipating fin 224, and the heat dissipating fan is disposed on a side of the base 21 facing away from the temperature changing block 22 and corresponding to the heat dissipating fin 224. It will be appreciated that heat is dissipated to the refrigerator 222 by the heat sink 224 and the heat dissipating fan to facilitate temperature changing of the first heat conductive plate 223.
In an embodiment, as shown in fig. 1,2, 3, 6 and 7, the temperature changing block 22 further includes a first protection switch 225 and a first temperature sensor 226, the first protection switch 225 is electrically connected to the first temperature sensor 226, the first heat insulation block 221 is provided with a first accommodating groove 2212 and a second accommodating groove 2213 at intervals, the first protection switch 225 is disposed in the first accommodating groove 2212, and the first temperature sensor 226 is disposed in the second accommodating groove 2213 and is abutted to the first heat conducting plate 223.
It can be appreciated that the first protection switch 225 and the first temperature sensor 226 are provided so that the temperature of the first heat conductive plate 223 is detected in real time by the first temperature sensor 226, thereby effectively controlling the temperature change of the refrigerator 222. In this embodiment, the first protection switch 225 is electrically connected to the refrigerator 222, and the working state of the refrigerator 222 can be controlled by the first protection switch 225 according to the detection condition of the first temperature sensor 226.
In this embodiment, the TEC is connected to the upper side of the first heat insulating block 221, and the heat sink 224 is connected to the lower side of the first heat insulating block 221, so that heat is transferred longitudinally in the first heat conducting plate 223-TEC-heat sink 224, and heat is isolated from being transferred to other parts laterally. It can be appreciated that, during heating, the heat released by the TEC is transferred to the local area of the first heat-conducting plate 223, and the reagent card is placed on the first heat-conducting plate 223 for heating because of the strong heat-conducting property of the first heat-conducting plate 223 and rapidly spreads to the whole area with a small temperature difference.
In an embodiment, as shown in fig. 1 to 5, the constant temperature block 23 includes a second heat insulation block 231, a heating film 232 and a second heat conducting plate 233, wherein the second heat insulation block 231 is disposed on the base 21 and is located at one end of the base 21 away from the temperature changing block 22, a second mounting groove 2311 is disposed on one side of the second heat insulation block 231 facing away from the base 21, the heating film 232 is disposed in the second mounting groove 2311, and the second heat conducting plate 233 is disposed on the second heat insulation block 231 and abuts against the heating film 232 to cover a notch of the second mounting groove 2311.
In the present embodiment, by disposing the heating film 232 in the second mounting groove 2311 of the second heat insulating block 231, on the one hand, the heat insulation of the base 21 is achieved by the second heat insulating block 231, and the limitation of the heating film 232 is achieved, on the other hand, the heat loss of the heating film 232 is effectively reduced. It can be appreciated that by providing the second heat conductive plate 233, it is convenient to use the second heat conductive plate 233 to rapidly transfer heat, and at the same time, to use the second heat conductive plate 233 to achieve temperature uniformity of the reagent card, so as to improve accuracy.
Optionally, a heat-conducting silicone grease is filled between the second heat-conducting plate 233 and the heating film 232, so that the heat conduction efficiency can be further improved. In the present embodiment, the heating film 232 is a resistance wire heating film. Optionally, the second heat-conducting plate 233 is an aluminum plate, and the material of the second heat-insulating block 231 is an epoxy resin material.
It is understood that the heating film 232 may be attached to the second heat conductive plate 233 and/or to the second heat insulating block 231 by 3M glue. In this embodiment, the second mounting groove 2311 of the second insulation block 231 also performs a heat cover function.
In this embodiment, the temperature changing block 22 is placed below the PCR cavity, and the TEC is used to control the denaturation (95 ℃) of the PCR cavity reagent, annealing and extension (60 ℃) so as to implement the PCR amplification reaction, the constant temperature block 23 is heated and maintained at 105 ℃ by the heating film 232 to cover other cavities connected with the PCR cavity, so that the constant temperature region 105 ℃ of the heating table 2 and the temperature changing region form a temperature difference, thereby being capable of avoiding the other cavities connected with the PCR from generating high temperature and high pressure, reducing the evaporation of the PCR cavity liquid, simultaneously inhibiting the precipitation of internal bubbles, reducing the interference of factors thereof on the optical detection, and implementing the thermal cover function in the reagent card.
In an embodiment, as shown in fig. 1 to 5, the constant temperature block 23 further includes a second protection switch 234 and a second temperature sensor 235, the second protection switch 234 is electrically connected to the second temperature sensor 235, the second insulation block 231 is provided with a first accommodating groove 2312 and a second accommodating groove 2313 at intervals, the second protection switch 234 is disposed in the first accommodating groove 2312, and the second temperature sensor 235 is disposed in the second accommodating groove 2313 and abuts against the second heat conducting plate 233.
It can be appreciated that by providing the second protection switch 234 and the second temperature sensor 235, the temperature of the second heat conductive plate 233 is detected in real time by the second temperature sensor 235, thereby effectively controlling the temperature change of the heating film 232. In this embodiment, the second protection switch 234 is electrically connected to the heating film 232, and the working state of the heating film 232 can be controlled by the second protection switch 234 according to the detection condition of the second temperature sensor 235.
In this embodiment, the heating film 232 is connected above the second heat insulating block 231, so that heat is transferred longitudinally in the second heat conducting plate 233-the heating film 232, and heat is insulated from being transferred laterally to other parts. It can be appreciated that, during heating, the heat released by the heating film 232 is transferred to the local area of the second heat-conducting plate 233, and the reagent card is placed on the second heat-conducting plate 233 for heating due to the strong heat-conducting property of the second heat-conducting plate 233 and rapid diffusion to the whole area with small temperature difference.
In one embodiment, as shown in fig. 1, the machine body 1 includes a first fixing plate 12 and a second fixing plate 13, where the first fixing plate 12 and the second fixing plate 13 are opposite and spaced apart to form an installation space 11; the pressing plate 3 is in sliding connection with the first fixing plate 12, and the heating table 2 is in sliding connection with the second fixing plate 13.
In the present embodiment, the pressing plate 3 is slidably connected to the first fixing plate 12, so that the first fixing plate 12 provides a guiding sliding action for the movement of the pressing plate 3 when the lifting assembly 4 drives the pressing plate 3 to move. By providing the heating table 2 in sliding connection with the second fixing plate 13, the second fixing plate 13 provides a guiding sliding action for the movement of the heating table 2 when the lifting assembly 4 drives the heating table 2 to move.
In one embodiment, one of the pressing plate 3 and the first fixing plate 12 is provided with a first sliding table 14, and the other is provided with a first sliding groove which is in sliding fit with the first sliding table 14. One of the heating table 2 and the second fixing plate 13 is provided with a second sliding table 15, and the other is provided with a second sliding groove 243, and the second sliding groove 243 is in sliding fit with the second sliding table 15.
In this embodiment, as shown in fig. 1, by respectively providing the first sliding groove and the first sliding table 14 on the pressing plate 3 and the first fixing plate 12, the first sliding groove is slidably matched with the first sliding table 14, so that the first sliding table 14 provides sliding guidance for the movement of the pressing plate 3, and the clamping phenomenon of the pressing plate 3 during the movement is avoided. It can be appreciated that by arranging the second sliding groove 243 and the second sliding table 15 on the heating table 2 and the second fixing plate 13 respectively, the second sliding groove 243 is utilized to slidably cooperate with the second sliding table 15, so that the second sliding table 15 provides sliding guiding for the movement of the heating table 2, and the clamping phenomenon of the heating table 2 during the movement is avoided.
In an embodiment, as shown in fig. 1, a connecting table 24 is disposed at an end of the heating table 2 away from the pressing plate 3, the connecting table 24 includes a sliding portion 241 and a connecting portion 242 disposed at an included angle, the sliding portion 241 is slidably connected to the second fixing plate 13, and the connecting portion 242 is connected to an output shaft of the lifting assembly 4.
It will be appreciated that the connection portion 242 of the connection stage 24 is positioned with the base 21 of the heating stage 2 by pins to ensure the flatness of the installation of the heating stage 2.
In an embodiment, the lifting assembly 4 includes a driving member 41, two synchronizing wheels 42 and a synchronous belt 43, wherein an output shaft of the driving member 41 is connected with the heating table 2, the two synchronizing wheels 42 are arranged on the first fixing plate 12 at intervals, the synchronous belt 43 is sleeved on the two synchronizing wheels 42, and the heating table 2 and the pressing plate 3 are connected with the synchronous belt 43; when the driving member 41 drives the heating table 2 to move, the heating table 2 drives the synchronous belt 43 to move, so that the heating table 2 and the pressing plate 3 are close to or far away from each other.
In this embodiment, the driving member 41 may be a driving motor, an output shaft of the driving motor is a screw, for example, a linear screw motor, and the output shaft of the driving motor is in threaded connection with the connecting portion 242 of the connecting table 24, so that when the driving motor drives the output shaft to rotate, the connecting portion 242 of the connecting table 24 drives the base 21 of the heating table 2 to move along the second sliding table 15.
It will be appreciated that the two synchronizing wheels 42 are provided on the first fixed plate 12, and the synchronizing belt 43 is sleeved on the two synchronizing wheels 42, such that the synchronizing belt 43 has a first belt section and a second belt section, and the first belt section and the second belt section are located on opposite sides of a line connecting the two synchronizing wheels 42. Through being connected with second band section and first band section respectively with heating platform 2 and clamp plate 3 for when driving piece 41 drive connecting portion 242 drove the base 21 of heating platform 2 and rise along second slip table 15, the second band section is the rise, and first band section is the decline, so that clamp plate 3 drove clamp plate 3 and descend, thereby makes heating platform 2 and clamp plate 3 be in mutual proximity.
In this embodiment, as shown in fig. 1, the pressing plate 3 is provided with a first connecting seat 33, the first connecting seat 33 is connected with the first belt section, one end of the heating table 2 adjacent to the pressing plate 3 is provided with a second connecting seat 25, and the second connecting seat 25 is connected with the second belt section.
When the linear screw motor of the PCR microfluidic temperature control device 100 works, the screw rotates to drive the connecting part 242 of the connecting table 24 to ascend along the second sliding table 15, drive the heating table 2 to ascend and synchronously transmit the motion to the second belt section of the synchronous belt 43, so that the second belt section of the synchronous belt 43 ascends, the first belt section descends, the pressing plate 3 is driven by the first belt section to descend, the temperature changing block 22 of the heating table 2 is attached to the lower surface of the reagent card, and the upper surface of the reagent card is attached to the pressing part 31 through the foam 32 of the pressing plate 3. The structure can increase the contact area between the lower surface of the reagent card and the heating table 2, reduce the heat dissipation area of the upper surface and increase the temperature rising and falling speed of the reagent card.
The foregoing description is only of the optional embodiments of the present invention, and is not intended to limit the scope of the invention, and all equivalent structural modifications made by the present description and accompanying drawings or direct/indirect application in other related technical fields are included in the scope of the present invention.
Claims (5)
1. A PCR microfluidic temperature control device, characterized in that the PCR microfluidic temperature control device comprises:
The machine body is provided with an installation space, and comprises a first fixing plate and a second fixing plate which are opposite and arranged at intervals so as to form the installation space in a surrounding manner;
The heating assembly comprises a heating table and a pressing plate, the pressing plate and the heating table are movably arranged in the installation space and are oppositely arranged, the heating table is used for containing and heating the reagent card, the pressing plate is in sliding connection with the first fixing plate, and the heating table is in sliding connection with the second fixing plate; and
The lifting assembly is connected with the heating table and/or the pressing plate to drive the heating table and/or the pressing plate to move so as to enable the heating table and the pressing plate to be close to or far away from each other to compress or release the reagent card, the lifting assembly comprises a driving piece, two synchronous wheels and a synchronous belt, an output shaft of the driving piece is connected with the heating table, the two synchronous wheels are arranged on the first fixed plate at intervals, the synchronous belt is sleeved on the two synchronous wheels, the heating table and the pressing plate are connected with the synchronous belt, and when the driving piece drives the heating table to move, the heating table drives the synchronous belt to move so as to enable the heating table and the pressing plate to be close to or far away from each other;
The heating table comprises a base, a temperature changing block and a constant temperature block, wherein the base is movably arranged in the installation space, the temperature changing block is arranged on the base and corresponds to the pressing plate to form a temperature changing area, the constant temperature block is arranged on the base and is separated from the temperature changing block to form a constant temperature area, the temperature changing block comprises a first heat insulating block, a refrigerator and a first heat conducting plate, the first heat insulating block is arranged on the base and corresponds to the pressing plate, a first installation groove is formed in one side of the first heat insulating block, the refrigerator is arranged in the first installation groove, the first heat conducting plate is arranged on the first heat insulating block to cover the notch of the first installation groove, the constant temperature block comprises a second heat insulating block, a heating film and a second heat conducting plate, the second heat insulating block is arranged on the base and is positioned at one end of the base far away from the temperature changing block, a second installation groove is formed in one side of the base, the second heat insulating block is arranged on the second heat insulating block, the second heat conducting plate is arranged in the second installation groove, the second heat insulating block is arranged in the second installation groove, and the second heat conducting plate is arranged in the second installation groove, and is provided with a second heat conducting plate.
2. The PCR microfluidic temperature control device of claim 1, wherein the heating station has the constant temperature zone and the variable temperature zone arranged at intervals;
the pressing plate comprises a pressing part and foam, the pressing part and the temperature changing area are movably arranged in the installation space, the pressing part is opposite to the temperature changing area, and the foam is arranged on one side of the pressing part facing the temperature changing area.
3. The PCR microfluidic temperature control device according to claim 2, wherein the temperature changing block further comprises a heat sink and a heat dissipating fan, the heat sink is arranged on one side of the first heat insulating block facing away from the first heat conducting plate, the base is provided with a via hole corresponding to the heat sink, and the heat dissipating fan is arranged on one side of the base facing away from the temperature changing block and is arranged corresponding to the heat sink;
and/or, the temperature changing block further comprises a first protection switch and a first temperature sensor, the first protection switch is electrically connected with the first temperature sensor, a first accommodating groove and a second accommodating groove are formed in the first heat insulating block at intervals, the first protection switch is arranged in the first accommodating groove, and the first temperature sensor is arranged in the second accommodating groove and is in butt joint with the first heat conducting plate;
and/or the refrigerator comprises a plurality of first heat insulation blocks, wherein a plurality of first mounting grooves are formed in the first heat insulation blocks, and each refrigerator is arranged in one first mounting groove;
and/or heat conduction silicone grease is filled between the first heat conduction plate and the refrigerator.
4. The PCR microfluidic temperature control device according to claim 2, wherein the thermostatic block further comprises a second protection switch and a second temperature sensor, the second protection switch and the second temperature sensor are electrically connected, the second heat insulation block is provided with a first accommodating groove and a second accommodating groove at intervals, the second protection switch is arranged in the first accommodating groove, and the second temperature sensor is arranged in the second accommodating groove and is abutted against the second heat conducting plate;
And/or heat conduction silicone grease is filled between the second heat conduction plate and the heating film.
5. The PCR microfluidic temperature control device according to any one of claims 1 to 4, wherein one of the pressure plate and the first fixing plate is provided with a first sliding table, and the other one of the pressure plate and the first fixing plate is provided with a first sliding groove which is in sliding fit with the first sliding table;
And/or, one of the heating table and the second fixing plate is provided with a second sliding table, and the other one of the heating table and the second fixing plate is provided with a second sliding groove which is in sliding fit with the second sliding table;
And/or, one end of the heating table far away from the pressing plate is provided with a connecting table, the connecting table comprises a sliding part and a connecting part, the sliding part is in sliding connection with the second fixing plate, and the connecting part is connected with an output shaft of the driving piece;
And/or, the hold-in range has first band section and second band section, first band section with the second band section is located two the opposite sides of synchronizing wheel line, the clamp plate is equipped with first connecting seat, first connecting seat with first band section is connected, the heating platform is adjacent the one end of clamp plate is equipped with the second connecting seat, the second connecting seat with the second band section is connected.
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