CN111790462A - Heating device for micro-fluidic detection - Google Patents
Heating device for micro-fluidic detection Download PDFInfo
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- CN111790462A CN111790462A CN202010811508.5A CN202010811508A CN111790462A CN 111790462 A CN111790462 A CN 111790462A CN 202010811508 A CN202010811508 A CN 202010811508A CN 111790462 A CN111790462 A CN 111790462A
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- 238000010438 heat treatment Methods 0.000 title claims abstract description 120
- 238000001514 detection method Methods 0.000 title claims abstract description 29
- 238000009434 installation Methods 0.000 claims abstract description 24
- 238000003825 pressing Methods 0.000 claims description 47
- 210000004907 gland Anatomy 0.000 claims description 31
- 238000001816 cooling Methods 0.000 claims description 9
- 239000004519 grease Substances 0.000 claims description 7
- 229920001296 polysiloxane Polymers 0.000 claims description 7
- 230000007704 transition Effects 0.000 claims description 7
- 230000017525 heat dissipation Effects 0.000 claims description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- 230000003014 reinforcing effect Effects 0.000 claims description 3
- 239000011810 insulating material Substances 0.000 claims description 2
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Abstract
The invention relates to the technical field of microfluidics, in particular to a heating device for microfluidic detection, which comprises a base, a heating module, a moving plate, a stepping motor platform, an air guide groove, a fan, a photoelectric switch and a fixing plate, wherein the base is provided with a heating module installation through groove for installing the heating module.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a heating device for microfluidic detection.
Background
Microfluidics is a technology for precisely controlling and controlling microscale fluids, and particularly relates to a technology for automatically completing the whole analysis process by integrating basic operation units of sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis on a microfluidic chip with the square centimeter. Due to the huge potential in the fields of biology, chemistry, medicine and the like, the PCR-based micro-fluidic chip has been developed into a new research field crossing the subjects of biology, chemistry, medicine, fluid, electronics, materials, machinery and the like, when the PCR (Polymerase Chain Reaction) is used for amplifying and detecting nucleic acid on the micro-fluidic chip, the PCR is the key of success or failure of detection, the PCR is composed of three steps of denaturation, annealing and extension, the specificity of the PCR-based micro-fluidic chip depends on oligonucleotide primers which are complementary with two ends of a target sequence, and the PCR-based micro-fluidic chip is similar to the natural replication process of DNA. Different reaction stages of PCR require different holding temperatures, DNA is denatured into single strands at 95 ℃, primers and single strands are combined according to the base complementary pairing principle at about 60 ℃, the optimal reaction temperature of DNA polymerase is reached when the temperature is adjusted to about 72 ℃, and the DNA polymerase catalyzes and synthesizes complementary strands from a hydroxyl end to a phosphate group direction along a template according to the base complementary pairing principle.
PCR equipment on the existing market is expensive, has a single function, and most of operation objects are PCR test tubes, can not be used for microfluidic chips, and can not be applied to microfluidic automatic detection equipment. Therefore, a heating device for microfluidic detection with simple structure, wide application range, integration capability, low cost and high temperature control precision is urgently needed in the market.
Disclosure of Invention
The invention aims to provide a heating device for microfluidic detection, which has the characteristics of simple structure, wide application range, integration, low cost, high temperature control precision and the like, and solves the problems of rough work, poor precision, complex structure, high production cost and unsuitability for practical microfluidic automatic detection equipment of the conventional PCR heating device.
In order to achieve the purpose, the invention adopts the following technical scheme:
a heating device for micro-fluidic detection comprises a base, a heating module, a moving plate, a stepping motor platform, an air guide groove, a fan, a photoelectric switch and a fixed plate, wherein the base is provided with a heating module installation through groove for installing the heating module, the heating module is installed at the heating module installation through groove part on the base and is connected with the base, the air guide groove is used for guiding heat dissipation air flow, the upper part of the air guide groove is connected with the heating module, the fan for generating the heat dissipation air flow is installed at the lower part of the air guide groove, the side surface of the base is provided with the fixed plate, the stepping motor platform is installed at the lower part of the fixed plate and is connected with the fixed plate through bolts, the moving plate is installed on the stepping motor platform and is connected with the stepping motor platform, the photoelectric switch is installed at the side surface part of the moving plate and is used for detecting and controlling the position of a, the stepping motor platform is connected with the heating module through the moving plate and drives the heating module to move up and down through the moving plate.
The heating module is used for heating and insulating the microfluidic chip and comprises a heat conducting sheet, a gland, a guide seat, a spring, a radiating fin, a heat conducting pipe, a heating sheet, a pressing sheet I, a refrigerating sheet, a pressing sheet II and a screw, wherein the heat conducting sheet is internally provided with a through groove structure with two open ends, the heating sheet for heating the heat conducting sheet is arranged in the through groove in the heat conducting sheet and is contacted with the heat conducting sheet, the lower part of the heating sheet is provided with the pressing sheet I and the pressing sheet II for compressing the refrigerating sheet, the refrigerating sheet is arranged between the pressing sheet I and the pressing sheet II for cooling the heating sheet, the gland is provided with a heat conducting sheet mounting groove for mounting the heat conducting sheet, the heat conducting sheet is arranged in the heat conducting sheet mounting groove arranged in the gland and is contacted with the gland through heat conducting silicone grease, the guide seat is provided with a gland mounting groove for mounting the gland, the heat conducting pipe is characterized in that the gland is arranged in a gland mounting groove formed in the guide seat and is in contact with the guide seat through heat conducting silicone grease, the upper part of the heat conducting pipe is in contact with the pressing sheet II and conducts heat of the pressing sheet II, and the lower part of the heat conducting pipe is provided with a radiating fin used for radiating the heat conducting pipe, and the radiating fin is connected with the heat conducting pipe.
The guide holder side processing have the buckle that is used for with the gland joint the side of gland offer the line hole of crossing that is used for drawing out heating plate and refrigeration piece wiring end and be used for with the guide holder on buckle cooperate with the slotted hole of installation, the gland install in the guide holder through buckle and slotted hole joint mode make the two link to each other.
The periphery four corners position of guide holder on install the guide post, the guide post fixed link to each other with the guide holder, processing has the internal thread hole in the guide post, installs the spring in the periphery of guide post, offers the guide post installation department that is used for the guide post installation on the base, the guide holder pass through the guide post and install the guide post installation department position on the base, the internal thread hole position of screw installation on the guide post and spacing in guide post installation department position with the guide post.
And a driving frame for driving the guide left lower part to move up and down is arranged at the guide left lower part, and a positioning hole is formed in the driving frame.
The motion board install and be used for driving the drive frame and reciprocate on the step motor platform, the motion board include diaphragm, riser, transition connecting plate and reference column, the diaphragm install at riser upper portion position and link to each other with riser vertical fixation, the lower part position of diaphragm fixed be provided with drive frame on the reference column of locating hole matched with installation, the transition connecting plate install at riser outside side position and with the riser is fixed continuous, offer the constant head tank that is used for photoelectric switch to detect the location in the side of riser.
The side of the heating plate is provided with a sensor hole, and a temperature sensor for detecting temperature is arranged in the sensor hole.
The gland and the guide seat are made of heat insulation materials.
The heat conducting strip, the pressing sheet I and the pressing sheet II are made of red copper.
And reinforcing rib plates are arranged on the transverse plate and the vertical plate and are respectively and fixedly connected with the transverse plate and the vertical plate.
The invention has the beneficial effects that: the heating device for micro-fluidic detection provided by the invention is scientific in overall structural design and convenient to produce, install and use, and mainly solves the problems of rough work, poor precision, complex structure, high production cost and unsuitability for practical micro-fluidic automatic detection equipment of the conventional PCR heating device through the installation layout of structural components such as a base, a heating module, a moving plate, a stepping motor platform and the like, so that the heating device for micro-fluidic detection provided by the invention is simple in overall structure, wide in application range, capable of being installed and used in an integrated manner, low in production cost and high in temperature control precision, and is suitable for micro-fluidic automatic detection equipment.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic overall front view of the present invention;
FIG. 3 is an overall cross-sectional structural schematic of the present invention;
FIG. 4 is a schematic perspective view of a heating module according to the present invention;
FIG. 5 is a schematic cross-sectional view of a heating module according to the present invention;
FIG. 6 is a schematic view showing the structure of a heating plate according to the present invention;
FIG. 7 is a schematic view of the construction of the gland of the present invention;
FIG. 8 is a schematic view of the structure of the guide base according to the present invention;
FIG. 9 is a front view of the sports board of the present invention;
FIG. 10 is a rear view of the sports board of the present invention;
the reference numbers in the figures are: 1-base, 2-heating module, 3-motion plate, 4-stepping motor platform, 5-air guide groove, 6-heat conducting sheet, 7-gland, 8-guide seat, 9-spring, 10-heat radiating sheet, 11-heat conducting pipe, 12-heating sheet, 13-pressing sheet I, 14-refrigerating sheet, 15-pressing sheet II, 16-fan, 17-photoelectric switch, 18-fixing plate, 19-screw, 20-guide column mounting part, 21-heating module mounting through groove, 301-positioning groove, 302-positioning column, 303-transverse plate, 304-vertical plate, 305-reinforcing rib plate, 306-transition connecting plate 601-sensor hole, 602-through groove, 701-slotted hole, 702-wire passing hole, sensor hole, 703-heat conducting sheet mounting grooves, 801-fasteners, 802-guide columns, 803-positioning holes and 804-driving frames.
Detailed Description
Specific example 1: the core of the invention is to provide a heating device for microfluidic detection, which mainly comprises a base 1, a heating module 2, a moving plate 3, a stepping motor platform 4 and other structural components, and the installation layout of the heating device solves the problems of rough work, poor precision, complex structure, high production cost and unsuitability for practical microfluidic automatic detection equipment of the existing PCR heating device. When an element is referred to as being "connected to" another element, it can be directly or indirectly connected to the other element. The terms "left" and "right" used in this specification to denote orientation are used with reference to a particular structure shown in the drawings, and do not constitute a limitation on the structure. As shown in the attached drawings 1-10, the heating device for microfluidic detection provided by the invention mainly comprises a base 1, a heating module 2, a moving plate 3, a stepping motor platform 4, an air guide groove 5, a fan 16, a photoelectric switch 17 and a fixing plate 18, wherein the base 1 is provided with a heating module installation through groove 21 for installing the heating module 2, the heating module 2 for heating a microfluidic chip is installed at the heating module installation through groove 21 on the base 1, the air guide groove 5 is used for guiding a heat dissipation air flow, the upper part of the air guide groove 5 is installed on the heating module 2, the fan 16 for generating the heat dissipation air flow is installed at the lower part of the air guide groove 5, the side surface of the base 1 is provided with the fixing plate 18 for installing the stepping motor platform 4, the stepping motor platform 4 is installed at the lower part of the fixing plate 18 through a bolt, and the moving plate 3 for driving the heating module 2 to move up and down and adjust is installed on, the photoelectric switch 17 for detecting the position of the moving plate 3 is installed at the side part of the moving plate 3, the stepping motor platform 4 is connected with the heating module 2 through the moving plate 3 and drives the heating module 2 to move up and down through the moving plate 3, the heating module 2 is used for heating and insulating the microfluidic chip, the stepping motor platform comprises a heat conducting sheet 6, a gland 7, a guide seat 8, a spring 9, a radiating fin 10, a heat conducting pipe 11, a heating sheet 12, a pressing sheet I13, a refrigerating sheet 14, a pressing sheet II15 and a screw 19, the heat conducting sheet 6 is internally provided with a through groove structure with two open ends, the heating sheet 12 for heating the heat conducting sheet 6 is installed in the through groove 602 in the heat conducting sheet 6, the lower part of the heating sheet 12 is provided with a pressing sheet I13 and a pressing sheet II15 for pressing the refrigerating sheet 14, the refrigerating sheet 14 is installed between the pressing sheet I13 and the pressing sheet II15 for cooling the heating sheet 12, the gland 7 is provided with a heat conducting sheet 6 installation groove 703 for installing, the heat conducting fin 6 is arranged in a heat conducting fin mounting groove 703 arranged in the gland 7 and is contacted with the gland 7 through heat conducting silicone grease, a gland mounting groove for mounting the gland 7 is arranged on the guide seat 8, the gland 7 is arranged in the gland mounting groove arranged on the guide seat 8 and is contacted with the guide seat 8 through heat conducting silicone grease, the upper part of the heat conducting pipe 11 is contacted with the pressing sheet II15 and conducts the heat of the pressing sheet II15, a radiating fin 10 for radiating the heat conducting pipe 11 is arranged at the lower part of the heat conducting pipe 11, a buckle 801 for clamping with the gland 7 is processed at the side surface of the guide seat 8, a wire passing hole 702 for leading out the terminals of the heating sheet 12 and the refrigerating sheet 14 and a slotted hole 701 for matching with the buckle 801 on the guide seat 8 are arranged at the side surface of the gland 7, the gland 7 is arranged in the guide seat 8 through the way of clamping the buckle 801 and the slotted hole 701, guide columns 802 for supporting and guiding the guide seat 8 are arranged at the lower, guide post 802 and guide holder 8 are fixed continuous, processing has the internal thread hole in guide post 802, install spring 9 on the periphery of guide post 802, spring 9 is buffer spring, its initial position is compressed by heating module 2 self gravity, when step motor platform 4 moves down through motion plate 3 drive heating module 2, spring 9 is in the state of compressed, when step motor platform 4 shifts up through motion plate 3 drive heating module 2, spring 9 resets by the elastic restoring force of self. A guide post mounting part 20 for mounting a guide post 820 is arranged on the base 1, a round smooth through hole is processed on the guide post mounting part 20, the guide post 802 is of a cylindrical structure with a smooth outer wall, and the diameter of the round smooth through hole is slightly larger than the outer diameter of the guide post 802. The guide base 8 is installed on the installation part of the guide post 802 on the base 1 through the guide post 802, the screw 19 is installed on the internal thread hole part on the guide post 802 and limits the guide post 802 on the installation part 20 of the guide post, the left lower part of the guide post 802 is installed with the driving frame 804 for driving the guide post to move up and down, the driving frame 804 is opened with a positioning hole 803, the moving plate 3 is installed on the stepping motor platform 4 for driving the driving frame 804 to move up and down, as shown in the specification and the attached drawings of fig. 9 and 10, the moving plate 3 mainly comprises a horizontal plate 303, a vertical plate 304, a transition connecting plate 306 and a positioning post 302, the horizontal plate 303 is fixedly installed on the upper part of the vertical plate 304, the positioning post 302 matched with the positioning hole 803 on the driving frame 804 is fixedly arranged on the lower part of the horizontal plate 303, when the positioning post 302 is assembled, transition connecting plate 306 is installed at riser 304 outside side position, and step motor platform 4 accessible transition connecting plate 306 drives and drives frame 804 and then drive guide holder 8 upper and lower position and move, is provided with sensor hole 601 in heating plate 12 side, installs the temperature sensor who is used for detecting heating plate 12 temperature in sensor hole 601. The invention is installed and used when using specifically, firstly, the integral structure of the heating device for micro-fluidic detection of the invention is as shown in the description attached figure 1, the description attached figure 2 and the description attached figure 3, the heating module 2 is movably sleeved on the base 1, the spring 9 is installed at the bottom of the heating module 2, the upper contact surface of the heat conducting strip 6 on the heating module 2 is about 2mm higher than the upper surface of the base 1 when the spring 9 is released freely, the micro-fluidic chip (not shown) is contacted with the upper surface of the base 1, when the micro-fluidic chip is fed from one side, the heating module 2 descends and abdicates first (the specific working process is that the stepping motor platform 4 drives the moving plate 3 to move downwards, so that the moving plate 3 drives the guide seat 8 to move downwards at the position of the guide post installation part 20 on the base 1 through the driving frame 804, at this time, the lower part of the guide post 802 moves downwards in the round smooth through hole processed on the guide post, at this moment, the guide holder 8 compresses the spring 9), and when the microfluidic chip enters the working position, the heating module 2 rises (the specific working process is: the motor feeding platform 4 drives the moving plate 3 to move upwards, so that the moving plate 3 drives the guide seat 8 to move upwards at the position of the guide post mounting part 20 on the base 1 through the driving frame 804, at the moment, the lower part of the guide post 802 moves upwards in the round smooth through hole processed on the guide post mounting part 20, at the moment, the spring 9 resets) to enable the heat conducting strip 6 on the motor to be in contact with the microfluidic chip, the contact is seamless, and the heating effect can be effectively guaranteed. The upper end of an air guide groove 5 is connected with a heating module 2, the heating module 2 can move up and down in the air guide groove 5, the lower end of the air guide groove 5 is connected with a fan 16, the fan 16 dissipates heat to the outside of the device, a stepping motor platform 4 is fixed on a base through a fixing plate 18, a moving plate 3 moves downwards to enable the heating module 2 to be placed at an initial position (the contact surface of the heating module is lower than the upper surface of the base 1) to avoid a micro-fluidic chip, when the micro-fluidic chip enters a working position, the heating module 2 is released to be in contact with the micro-fluidic chip by moving upwards, and a photoelectric switch 17 controls. The heating module 2 of the invention is as shown in the specification attached figure 4, the specification attached figure 5 and the specification attached figure 6, the heat conducting sheet 6 is made of red copper and is in direct contact with the microfluidic chip, the heating sheet 12 is arranged in the heat conducting sheet, the heating mode is electric heating, the pressing sheet I13 is arranged below the heating sheet 12 and plays a role of pressing, the refrigerating sheet 14 is arranged below the pressing sheet I13 and plays a role of quickly cooling, the pressing sheet II15 is arranged below the refrigerating sheet and plays a role of pressing, the heat conducting pipe 11 is arranged below the pressing sheet II15, the parts are all arranged in the pressing cover 7 and the guide seat 8, the clearance is filled with heat conducting silicone grease, the tail end of the heat conducting pipe 11 is provided with the radiating fin 10, the heating sheet 12 and the refrigerating sheet 14 are both connected with two wires, as shown in the specification attached figure 7, the pressing cover 7 and the guide seat 8 are made of heat insulating material, the pressing sheet I13 and the pressing sheet II15 are made, the micro-fluidic chip moving device can move up and down, the moving down of the micro-fluidic chip moving device is realized by driving the guide seat 8 by the stepping motor platform 4 through the moving plate 3, the moving down enables the contact surface of the heat conducting strip 6 to be lower than the upper surface of the base 1, the micro-fluidic chip is prevented from entering, the moving up is realized by the spring 9, and the contact surface of the heat conducting strip 6 is in seamless contact with the micro-fluidic. As shown in fig. 8, 4 guide posts 802 are disposed at the bottom of the guide base 8, and are installed in the guide holes on the base 1, the spring 9 is arranged on the guide post 802, the internal thread is arranged in the guide post 802 and is matched with the screw 19 for limiting, the adjusting screw 19 can adjust the pressure between the heating module 2 and the chip, the heating and the cooling in the heating module 2 are realized by different devices, the heating by the heating sheet 12 and the cooling by the cooling sheet 14 are respectively realized (the heating principle of the heating sheet 12 and the cooling principle of the cooling sheet 14 belong to the prior known technology, and the description is not repeated here), can effectively improve the reaction speed and temperature control precision of temperature regulation, ensure the effective performance of PCR, adjust the heating area of the heating module 2 according to the area of a PCR area, meanwhile, the sizes of the heating plate 12, the refrigerating plate 14 and the gasket are adjusted to ensure the optimal temperature control effect, and the invention is also suitable for simultaneously heating the parallel multi-channel microfluidic chips. The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.
Claims (10)
1. A heating device for micro-fluidic detection is characterized by comprising a base, a heating module, a moving plate, a stepping motor platform, an air guide groove, a fan, a photoelectric switch and a fixing plate, wherein the base is provided with a heating module installation through groove for installing the heating module, the heating module is installed at the heating module installation through groove part on the base and is connected with the base, the air guide groove is used for guiding heat dissipation air flow, the upper part of the air guide groove is connected with the heating module, the fan for generating the heat dissipation air flow is installed at the lower part of the air guide groove, the side surface of the base is provided with the fixing plate, the stepping motor platform is installed at the lower part of the fixing plate and is connected with the fixing plate through bolts, the moving plate is installed on the stepping motor platform and is connected with the stepping motor platform, the photoelectric switch is installed at the side surface part of the moving plate and is used for detecting and controlling the position of a, the stepping motor platform is connected with the heating module through the moving plate and drives the heating module to move up and down through the moving plate.
2. The heating device for microfluidic detection according to claim 1, wherein the heating module is used for heating and keeping the temperature of the microfluidic chip, and comprises a heat conducting plate, a pressing cover, a guide seat, a spring, a heat sink, a heat conducting pipe, a heating plate, a pressing plate I, a refrigerating plate, a pressing plate II and screws, the heat conducting plate is provided with a through groove structure with two open ends, the heating plate for heating the heat conducting plate is arranged in the through groove of the heat conducting plate and contacts with the heat conducting plate, the pressing plate I and the pressing plate II for pressing the refrigerating plate are arranged at the lower part of the heating plate, the refrigerating plate is arranged between the pressing plate I and the pressing plate II for cooling the heating plate, the pressing cover is provided with a heat conducting plate mounting groove for mounting the heat conducting plate, the heat conducting plate is arranged in the heat conducting plate mounting groove arranged in the pressing cover and contacts with the pressing cover through a heat conducting silicone grease, the guide seat is provided with a gland mounting groove for mounting a gland, the gland is mounted in the gland mounting groove formed in the guide seat and is in contact with the guide seat through heat-conducting silicone grease, the upper part of the heat-conducting pipe is in contact with the pressing sheet II and conducts heat of the pressing sheet II, the lower part of the heat-conducting pipe is provided with a radiating fin for radiating the heat-conducting pipe, and the radiating fin is connected with the heat-conducting pipe.
3. The heating device for microfluidic detection according to claim 1, wherein a buckle for clamping with the gland is processed on the side surface of the guide seat, a wire passing hole for leading out a terminal of the heating plate and the refrigerating plate and a slot hole for matching with the buckle on the guide seat are formed on the side surface of the gland, and the gland is installed in the guide seat and connected with the guide seat in a clamping manner of the buckle and the slot hole.
4. The heating device for microfluidic detection according to claim 1, wherein guide posts are installed at four corners of the outer periphery of the guide seat, the guide posts are fixedly connected with the guide seat, internal thread holes are processed in the guide posts, springs are installed at the outer periphery of the guide posts, guide post installation parts for installing the guide posts are arranged on the base, the guide seat is installed at the guide post installation parts on the base through the guide posts, and the guide posts are limited at the guide post installation parts by screws installed at the internal thread holes on the guide posts.
5. The heating device for microfluidic detection according to claim 1, wherein a driving frame for driving the guide seat to move up and down is installed at a lower portion of the guide seat, and a positioning hole is formed in the driving frame.
6. The heating device for micro-fluidic detection according to claim 1, characterized in that, the motion board install and be used for driving the drive frame on the step motor platform and reciprocate, the motion board include diaphragm, riser, transition connecting plate and reference column, the diaphragm install at riser upper portion position and link to each other with riser vertical fixation, the lower part position of diaphragm fixed be provided with drive frame go up the reference column of locating hole matched with installation, the transition connecting plate install at riser outside side position and with riser fixed linking to each other, offer the constant head tank that is used for photoelectric switch to detect the location in the side of riser.
7. The heating device for microfluidic detection according to claim 2, wherein the heat conducting sheet has a sensor hole on a side surface thereof, and a temperature sensor for detecting temperature is installed in the sensor hole.
8. The heating device for microfluidic detection according to claim 2, wherein the material of the pressing cover and the guiding seat is heat-insulating material.
9. The heating device for microfluidic detection according to claim 2, wherein the heat conducting sheet, the pressing sheet I and the pressing sheet II are made of red copper.
10. The heating device for microfluidic detection according to claim 6, wherein the transverse plate and the vertical plate are provided with reinforcing rib plates, and the reinforcing rib plates are respectively and fixedly connected with the transverse plate and the vertical plate.
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| CN202010811508.5A CN111790462B (en) | 2020-08-13 | 2020-08-13 | Heating device for microfluidic detection |
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| CN202010811508.5A CN111790462B (en) | 2020-08-13 | 2020-08-13 | Heating device for microfluidic detection |
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