CN111790462B - Heating device for microfluidic detection - Google Patents
Heating device for microfluidic detection Download PDFInfo
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- CN111790462B CN111790462B CN202010811508.5A CN202010811508A CN111790462B CN 111790462 B CN111790462 B CN 111790462B CN 202010811508 A CN202010811508 A CN 202010811508A CN 111790462 B CN111790462 B CN 111790462B
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- 238000010438 heat treatment Methods 0.000 title claims abstract description 109
- 238000001514 detection method Methods 0.000 title claims abstract description 28
- 238000003825 pressing Methods 0.000 claims description 47
- 210000004907 gland Anatomy 0.000 claims description 24
- 238000009434 installation Methods 0.000 claims description 21
- 238000001816 cooling Methods 0.000 claims description 12
- 230000007704 transition Effects 0.000 claims description 7
- 239000004519 grease Substances 0.000 claims description 5
- 229920001296 polysiloxane Polymers 0.000 claims description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052802 copper Inorganic materials 0.000 claims description 4
- 239000010949 copper Substances 0.000 claims description 4
- 230000003014 reinforcing effect Effects 0.000 claims description 4
- 239000011810 insulating material Substances 0.000 claims description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 14
- 230000006835 compression Effects 0.000 description 8
- 238000007906 compression Methods 0.000 description 8
- 230000017525 heat dissipation Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000003155 DNA primer Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- -1 electronics Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000013615 primer Substances 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention relates to the technical field of microfluidics, in particular to a heating device for microfluidic detection, which comprises a base, a heating module, a moving plate, a stepping motor platform, an air guide groove, a fan, a photoelectric switch and a fixing plate, wherein the base is provided with a heating module mounting through groove for mounting the heating module.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a heating device for microfluidic detection.
Background
Microfluidic is a technology for precisely controlling and manipulating microscale fluid, and specifically is a technology for integrating basic operation units such as sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes onto a microfluidic chip with a square centimeter, and automatically completing the whole analysis process. Because of its great potential in biological, chemical, medical and other fields, it has been developed into a new research field where subjects of biology, chemistry, medicine, fluid, electronics, materials, machinery and the like cross, and when detecting nucleic acids by using PCR (polymerase chain reaction ) amplification on a microfluidic chip, PCR is a key to detecting success or failure, and PCR is composed of three steps of denaturation-annealing-extension, whose specificity depends on oligonucleotide primers complementary to both ends of a target sequence, similar to the natural replication process of DNA. The different reaction stages of PCR require different holding temperatures, DNA becomes single-stranded at 95 ℃, primers bind to the single-stranded according to the base complementary pairing principle at about 60 ℃, and when the temperature is adjusted to about 72 ℃, the optimum reaction temperature of DNA polymerase is reached, and the DNA polymerase catalyzes and synthesizes the complementary strand from the hydroxyl end to the phosphate group along the template according to the base complementary pairing principle.
The PCR equipment in the current market is high in price and single in function, the operation objects are mostly PCR test tubes, the operation objects cannot be used for microfluidic chips, and the operation objects cannot be suitable for microfluidic automatic detection equipment, and most of the PCR heating devices adopted by the microfluidic chips at present are mostly used in laboratories, and the heating devices are either coarser, poor in precision, or complex in structure and high in production cost, and are not suitable for the practical microfluidic automatic detection equipment. Therefore, a heating device for microfluidic detection, which has the advantages of simple structure, wide application range, integration, low cost and high temperature control precision, is urgently needed in the market.
Disclosure of Invention
The invention aims to provide a heating device for microfluidic detection, which has the characteristics of simple structure, wide application range, low cost, high temperature control precision and the like, and solves the problems of rough work, poor precision, complex structure and high production cost of the traditional PCR heating device, and is not suitable for the use of practical microfluidic automatic detection equipment.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a micro-fluidic heating device for detecting, includes, base, heating module, movable plate, step motor platform, wind-guiding groove, fan, photoelectric switch and fixed plate, the base on set up the heating module installation logical groove that is used for the heating module installation, heating module install logical groove position and link to each other with the base on the base, the wind-guiding groove be used for carrying out the water conservancy diversion to the heat dissipation air current, the upper portion of wind-guiding groove link to each other with heating module, the fan for producing the heat dissipation air current install the lower part at the wind-guiding groove, the base side-mounting have the fixed plate, step motor platform install the lower part at the fixed plate and pass through the bolt with the fixed plate and link to each other, the movable plate install on the step motor platform and link to each other with the step motor platform, photoelectric switch install and be used for detecting the control to the position of movable plate side position, the step motor platform pass through the movable plate and link to each other and drive the heating module through the movable plate and reciprocate.
The heating module is used for heating and heat-preserving operation on the microfluidic chip and comprises a heat conducting fin, a gland, a guide seat, a spring, a radiating fin, a heat conducting tube, a heating fin, a pressing piece I, a refrigerating fin, a pressing piece II and a screw, wherein the heat conducting fin is internally provided with a through groove structure with two open ends, the heating fin for heating the heat conducting fin is arranged in the through groove in the heat conducting fin and is in contact with the heat conducting fin, the lower part of the heating fin is provided with the pressing piece I and the pressing piece II for pressing the refrigerating fin, the position between the pressing piece I and the pressing piece II is used for cooling the heat conducting fin, the pressing cover is provided with a heat conducting fin mounting groove for mounting the heat conducting fin, the heat conducting fin is arranged in the heat conducting fin mounting groove formed in the pressing cover and is in contact with the pressing cover through heat conducting silicone grease, the guide seat is provided with a mounting groove for mounting the pressing cover, the pressing cover is arranged in the pressing cover mounting groove formed in the guide seat and is in contact with the guide seat, the upper part of the heat conducting fin is in contact with the upper part of the heat conducting tube and the heat conducting fin is used for cooling the radiating fin which is connected with the heat conducting tube.
The side of the guide seat is provided with a buckle for being clamped with the gland, the side of the gland is provided with a wire passing hole for leading out the wiring ends of the heating plate and the refrigerating plate and a slot hole for being matched with the buckle on the guide seat, and the gland is arranged in the guide seat and is connected with the heating plate and the refrigerating plate in a clamping way through the buckle and the slot hole.
The guide post is installed on the periphery four-angle position of the guide seat, the guide post is fixedly connected with the guide seat, an internal thread hole is formed in the guide post, a spring is installed on the periphery of the guide post, a guide post installation part for installing the guide post is arranged on the base, the guide seat is installed on the base through the guide post, and the screw is installed at the internal thread hole part on the guide post and limits the guide post at the guide post installation part.
The left lower part of the guide is provided with a driving frame for driving the guide to move up and down, and the driving frame is provided with a positioning hole.
The motion board install and be used for driving the drive frame and reciprocate on step motor platform, the motion board include diaphragm, riser, transition connecting plate and reference column, the diaphragm install in riser upper portion position and with riser vertical fixation link to each other, the fixed reference column that installs that is provided with in lower part position of diaphragm cooperatees with the locating hole on the drive frame, transition connecting plate install at riser outside side position and with the riser fixed continuous, offered the constant head tank that is used for photoelectric switch to detect the location in the side of riser.
The side of the heating plate is provided with a sensor hole, and a temperature sensor for detecting temperature is arranged in the sensor hole.
The gland and the guide seat are made of heat insulation materials.
The heat conducting fin, the pressing piece I and the pressing piece II are made of red copper.
Reinforcing rib plates are arranged on the transverse plates and the vertical plates, and the reinforcing rib plates are fixedly connected with the transverse plates and the vertical plates respectively.
The beneficial effects of the invention are as follows: the heating device for microfluidic detection mainly solves the problems of rough work, poor precision, complex structure and high production cost of the traditional PCR heating device and is not suitable for the use of practical microfluidic automatic detection equipment by the installation layout of structural components such as a base, a heating module, a moving plate, a stepping motor platform and the like, and therefore, the heating device for microfluidic detection has the advantages of simple integral structure, wide application range, integrated installation and use, low production cost and high temperature control precision and is suitable for the microfluidic automatic detection equipment.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the overall front view of the present invention;
FIG. 3 is a schematic overall cross-sectional view of the present invention;
FIG. 4 is a schematic perspective view of a heating module according to the present invention;
FIG. 5 is a schematic cross-sectional view of a heating module of the present invention;
FIG. 6 is a schematic view of the structure of a heating plate of the present invention;
FIG. 7 is a schematic view of the structure of the gland of the present invention;
FIG. 8 is a schematic view of the structure of the guide holder of the present invention;
FIG. 9 is a schematic diagram of the front view of the kinematic plate of the present invention;
FIG. 10 is a schematic rear view of the motion plate of the present invention;
the reference numerals in the figures are: the device comprises a 1-base, a 2-heating module, a 3-moving plate, a 4-stepping motor platform, a 5-air guide groove, a 6-heat conducting fin, a 7-gland, an 8-guide seat, a 9-spring, a 10-radiating fin, an 11-heat conducting tube, a 12-heating fin, a 13-pressing piece I, a 14-refrigerating fin, a 15-pressing piece II, a 16-fan, a 17-photoelectric switch, an 18-fixing plate, a 19-screw, a 20-guide column installation part, a 21-heating module installation through groove, a 301-locating groove, a 302-locating column, a 303-transverse plate, a 304-vertical plate, a 305-reinforcing rib plate, a 306-transition connecting plate, a 601-sensor hole, a 602-through groove, a 701-slot hole, a 702-wire passing hole, a 703-heat conducting fin installation groove, a 801-buckle, an 802-guide column, a 803-locating hole and a 804-driving frame.
Detailed Description
Specific example 1: the invention aims at providing a heating device for microfluidic detection, which mainly comprises a base 1, a heating module 2, a moving plate 3, a stepping motor platform 4 and other structural components, so that the heating device solves the problems of rough work, poor precision, complex structure and high production cost of the traditional PCR heating device, and is not suitable for the use of practical microfluidic automatic detection equipment. When an element is referred to as being "connected to" another element, it can be directly or indirectly connected to the other element. The terms "left" and "right" used in this specification to denote orientations are each referenced to a particular structure shown in the drawings, and are not to be construed as limiting the structure. As shown in fig. 1-10 of the specification, the heating device for microfluidic detection provided by the invention mainly comprises a base 1, a heating module 2, a moving plate 3, a stepping motor platform 4, an air guide groove 5, a fan 16, a photoelectric switch 17 and a fixed plate 18, wherein a heating module installation through groove 21 for installing the heating module 2 is arranged on the base 1, the heating module 2 for heating a microfluidic chip is arranged on the base 1, the air guide groove 5 is used for guiding heat dissipation air flow, the upper part of the air guide groove 5 is arranged on the heating module 2, the fan 16 for generating heat dissipation air flow is arranged on the lower part of the air guide groove 5, the fixed plate 18 for installing the stepping motor platform 4 is arranged on the side surface of the base 1, the stepping motor platform 4 is arranged on the lower part of the fixed plate 18 through bolts, the moving plate 3 for driving the heating module 2 to move up and down and adjusting is arranged on the stepping motor platform 4, the photoelectric switch 17 for detecting the position of the moving plate 3 is arranged at the side surface part of the moving plate 3, the stepping motor platform 4 is connected with the heating module 2 through the moving plate 3 and drives the heating module 2 to move up and down through the moving plate 3, the heating module 2 is used for heating and preserving heat of a micro-fluidic chip and comprises a heat conducting sheet 6, a gland 7, a guide seat 8, a spring 9, a heat radiating sheet 10, a heat conducting tube 11, a heating sheet 12, a pressing sheet I13, a refrigerating sheet 14, a pressing sheet II15 and a screw 19, the heat conducting sheet 6 is of a through groove structure with two open ends, the heating sheet 12 for heating the heat conducting sheet 6 is arranged in a through groove 602 in the heat conducting sheet 6, pressing sheets I13 and II15 for pressing the refrigerating sheet 14 are arranged at the lower part of the heating sheet 12, the refrigerating fin 14 is installed and is set up in the position between preforming I13 and preforming II15 and is used for cooling to heating fin 12, set up a conducting fin 6 mounting groove 703 that is used for conducting fin 6 installation on gland 7, conducting fin 6 installs in conducting fin mounting groove 703 that sets up in gland 7 and contacts with gland 7 through heat conduction silicone grease, set up the gland mounting groove that is used for gland 7 installation on guide holder 8, gland 7 installs in the gland mounting groove that sets up on guide holder 8 and contacts with guide holder 8 through heat conduction silicone grease, the upper portion of heat pipe 11 contacts with preforming II15 and conducts the heat of preforming II15 away, install the fin 10 that is used for carrying out the heat dissipation to heat pipe 11 in the lower part of heat pipe 11, be processed at guide holder 8 side and be used for with the buckle 801 of gland 7 joint, the slotted hole 702 that is used for drawing out heating fin 12 and refrigerating fin 14 wiring terminal and the cooperation installation on guide holder 8 is seted up at the side of gland 7, 7 passes through buckle 801 and slotted hole 701 and slotted hole card joint mode installation in guide holder 8, install in guide holder 8 motor 802 and contact with slotted hole 802, install the guide holder 802 and the compression spring post 9 is equipped with the compression spring 9 when the compression spring holder 2 is in the compression spring holder 2 is installed to the side of guide post 2, the compression roller 9 is in the initial position of compression roller 9, the compression roller 9 is installed to the compression roller 9 when the roller 9 is in the position of roller 9. A guide post installation part 20 for installing the guide post 820 is arranged on the base 1, a circular smooth through hole is processed on the guide post installation part 20, the guide post 802 is of a cylindrical structure with a smooth outer wall, and the diameter of the circular smooth through hole is slightly larger than the outer diameter of the guide post 802. The guide seat 8 is arranged at the position of a guide post 802 mounting part on the base 1 through the guide post 802, the screw 19 is arranged at an internally threaded hole part on the guide post 802 and limits the guide post 802 at a guide post mounting part 20, a driving frame 804 for driving the guide post 802 to move up and down is arranged at the left lower part of the guide post 802, a positioning hole 803 is formed in the driving frame 804, the moving plate 3 is arranged on the stepping motor platform 4 and used for driving the driving frame 804 to move up and down, as shown in the specification of fig. 9 and 10, the moving plate 3 mainly comprises a transverse plate 303, a vertical plate 304, a transition connecting plate 306 and a positioning post 302, the transverse plate 303 is fixedly arranged at the upper part of the vertical plate 304, the positioning post 302 which is matched with the positioning hole 803 on the driving frame 804 is fixedly arranged at the lower part of the transverse plate 303, the positioning post 302 is arranged at the positioning hole part formed in the driving frame 804 when the assembly and use, the transition connecting plate 306 is arranged at the outer side part of the vertical plate 304, the stepping motor platform 4 can drive the driving frame 804 to move up and down through the driving frame 804, and then the guide seat 8 is driven to move up and down through the transition connecting plate 306, the sensor 601 is arranged at the side face of the heating plate 12, and the sensor 601 is provided with a temperature sensor hole 601 for detecting temperature sensor 601. The whole structure of the heating device for microfluidic detection is shown in the specification as shown in the accompanying drawing 1, the specification as shown in the accompanying drawing 2 and the specification as shown in the accompanying drawing 3, the heating module 2 is movably sleeved on the base 1, the spring 9 is arranged at the bottom of the heating module 2, the upper contact surface of the heat conducting fin 6 on the heating module 2 is higher than the upper surface of the base 1 by about 2mm when the spring 9 is released freely, a microfluidic chip (not shown in the drawing) is contacted with the upper surface of the base 1, when the microfluidic chip enters from one side, the heating module 2 firstly descends and yields (the specific working process is that the stepping motor platform 4 drives the moving plate 3 to move downwards, and then the moving plate 3 drives the guide seat 8 to move downwards at the guide column mounting part 20 on the base 1 through the driving frame 804, and then the guide seat 8 compresses the spring 9 at the moment), and when the microfluidic chip enters into the working position, the heating module 2 ascends (the specific working process is that the motor platform 4 drives the moving plate 3 to move upwards through the driving the moving plate 3 to move downwards at the guide column mounting part 20, and then the guide column mounting part is in contact with the guide column mounting part 20, and the upper guide column is in contact with the guide column mounting part of the guide seat 3, and the guide column is in the upper contact with the guide column mounting part of the guide column mounting part is in the round smooth through the guide column mounting part 20, and the guide column is in the round smooth hole of the guide plate mounting part is in the round smooth position. The upper end of the air guide groove 5 is connected with the heating module 2, the heating module 2 can move up and down in the air guide groove 5, the lower end of the air guide groove 5 is connected with the fan 16, the fan 16 dissipates heat outside the device, the stepping motor platform 4 is fixed on the base through the fixing plate 18, the moving plate 3 moves downwards to enable the heating module 2 to be placed at an initial position (the contact surface of the moving plate is lower than the upper surface of the base 1) so as to avoid the micro-fluidic chip, when the micro-fluidic chip enters a working position, the heating module 2 is released to be contacted with the micro-fluidic chip by upward movement, and the photoelectric switch 17 controls the stop position of the moving plate 3. According to the invention, a heating module 2 is shown in an attached drawing 4 in the specification, an attached drawing 5 in the specification and an attached drawing 6 in the specification, a heat conducting fin 6 is made of red copper material and is in direct contact with a microfluidic chip, a heating fin 12 is arranged in the heat conducting fin, the heating mode is electric heating, a pressing piece I13 is arranged below the heating fin 12, the pressing effect is achieved, a refrigerating fin 14 is arranged below the pressing piece I13, the rapid cooling effect is achieved, a pressing piece II15 is arranged below the refrigerating fin, the pressing effect is achieved, a heat conducting pipe 11 is arranged below the pressing piece II15, the parts are all arranged in a gland 7 and a guide seat 8, heat conducting silicone grease is filled in a gap, a radiating fin 10 is arranged at the tail end of the heat conducting pipe 11, the heating fin 12 and the refrigerating fin 14 are both connected with each other through a wire hole 702 on the gland 7 in the specification, the gland 7 and the guide seat 8 are made of heat insulating materials, the pressing piece I13 and the pressing piece II are made of red copper material, the heating module 2 is integrally arranged, the heating module can move up and down, the cooling is achieved by a stepping motor platform 4 through a moving plate 3, the guide seat 8 is driven by the stepping motor 8, the lower than the base 6 is enabled to be in contact with the upper surface of the microfluidic chip, the heat conducting surface is enabled to be in a state, and the micro-channel chip is enabled to be in a seamless contact with the micro-channel, and the micro-channel chip is enabled to be in a seamless contact with the surface through the micro-channel, and the micro-channel is realized. As shown in fig. 8 of the specification, 4 guide posts 802 are arranged at the bottom of the guide seat 8 and are installed in guide holes on the base 1, springs 9 are arranged on the guide posts, internal threads are arranged in the guide posts 802 and are matched with screws 19 to limit, the adjusting screws 19 can adjust the pressure between the heating module 2 and the chip, the heating and cooling in the heating module 2 are realized by different devices, the heating by the heating plate 12 is realized, the cooling by the cooling plate 14 is realized (the heating principle of the heating plate 12 and the cooling principle of the cooling plate 14 belong to the prior known technology and are not described again), the reaction speed and the temperature control precision of temperature regulation can be effectively improved, the effective performance of PCR (polymerase chain reaction) is ensured, the heating area of the heating module 2 can be adjusted according to the area of a PCR zone, and the sizes of the heating plate 12, the cooling plate 14 and a gasket are adjusted at the same time, so that the optimal temperature control effect is ensured. The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims.
Claims (10)
1. The heating device for microfluidic detection is characterized by comprising a base, a heating module, a moving plate, a stepping motor platform, an air guide groove, a fan, a photoelectric switch and a fixing plate, wherein the base is provided with a heating module installation through groove for installing the heating module;
the stepping motor platform drives the moving plate to move downwards, so that the moving plate drives the guide seat to move downwards at the guide column installation part of the base through the driving frame, and at the moment, the lower part of the guide column moves downwards in a circular smooth through hole machined in the guide column installation part;
the stepping motor platform moves upwards by driving the moving plate, and then the moving plate drives the guide seat to move upwards at the guide column installation part position on the base through the driving frame, and at the moment, the lower part of the guide column moves upwards in a circular smooth through hole machined in the guide column installation part.
2. The heating device for microfluidic detection according to claim 1, wherein the heating module is used for heating and heat-preserving the microfluidic chip, and comprises a heat conducting sheet, a gland, a guide seat, a spring, a radiating sheet, a heat conducting pipe, a heating sheet, a pressing sheet I, a refrigerating sheet, a pressing sheet II and a screw, wherein the heat conducting sheet is internally provided with a through groove structure with two open ends, the heating sheet for heating the heat conducting sheet is arranged in the through groove in the heat conducting sheet and is in contact with the heat conducting sheet, the pressing sheet I and the pressing sheet II for pressing the refrigerating sheet are arranged at the lower part of the heating sheet, the refrigerating sheet is arranged at a position between the pressing sheet I and the pressing sheet II for cooling the heating sheet, a heat conducting sheet mounting groove for mounting the heat conducting sheet is formed in the pressing sheet, the heat conducting sheet is arranged in the pressing sheet mounting groove and is in contact with the pressing sheet through heat conducting silicone grease, the guide seat is provided with a pressing cover mounting groove for mounting the pressing sheet, the pressing sheet is arranged in the guide seat and is in contact with the pressing sheet, the pressing sheet II is arranged in the guide seat, and the heat conducting sheet II is in contact with the heat conducting pipe through the guide seat, and the heat conducting pipe is in contact with the radiating part of the heat conducting pipe is arranged on the pressing sheet II.
3. The heating device for microfluidic detection according to claim 2, wherein the side surface of the guide seat is provided with a buckle for clamping with a gland, the side surface of the gland is provided with a wire passing hole for leading out the heating plate and the wiring terminal of the refrigerating plate and a slot hole for matching and installing the buckle on the guide seat, and the gland is arranged in the guide seat and connected with the guide seat in a clamping way of the buckle and the slot hole.
4. The heating device for microfluidic detection according to claim 3, wherein the guide post is mounted at a four-angle position on the outer periphery of the guide holder, the guide post is fixedly connected with the guide holder, an internal threaded hole is processed in the guide post, a spring is mounted on the outer periphery of the guide post, a guide post mounting part for mounting the guide post is arranged on the base, the guide holder is mounted at a guide post mounting part position on the base through the guide post, and the screw is mounted at the internal threaded hole part on the guide post and limits the guide post at the guide post mounting part.
5. The heating device for microfluidic detection according to claim 4, wherein a driving frame for driving the guiding seat to move up and down is installed at the lower part of the guiding seat, and a positioning hole is formed in the driving frame.
6. The heating device for microfluidic detection according to claim 5, wherein the moving plate is mounted on the stepping motor platform and used for driving the driving frame to move up and down, the moving plate comprises a transverse plate, a vertical plate, a transition connecting plate and a positioning column, the transverse plate is mounted on the upper portion of the vertical plate and is vertically and fixedly connected with the vertical plate, the positioning column which is matched with the positioning hole on the driving frame and mounted is fixedly arranged on the lower portion of the transverse plate, the transition connecting plate is mounted on the outer side portion of the vertical plate and is fixedly connected with the vertical plate, and a positioning groove for detecting and positioning of the photoelectric switch is formed in the side face of the vertical plate.
7. The heating device for microfluidic detection according to claim 2, wherein the heat conductive sheet is provided with a sensor hole on a side surface thereof, and a temperature sensor for detecting a temperature is installed in the sensor hole.
8. The heating device for microfluidic detection according to claim 2, wherein the gland and the guide holder are made of heat-insulating materials.
9. The heating device for microfluidic detection according to claim 2, wherein the heat conducting strip, the pressing sheet I and the pressing sheet II are made of red copper.
10. The heating device for microfluidic detection according to claim 6, wherein reinforcing rib plates are mounted on the transverse plates and the vertical plates, and the reinforcing rib plates are fixedly connected with the transverse plates and the vertical plates respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010811508.5A CN111790462B (en) | 2020-08-13 | 2020-08-13 | Heating device for microfluidic detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010811508.5A CN111790462B (en) | 2020-08-13 | 2020-08-13 | Heating device for microfluidic detection |
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| CN111790462A CN111790462A (en) | 2020-10-20 |
| CN111790462B true CN111790462B (en) | 2024-01-09 |
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