CN213924851U - Single cell nucleic acid processing instrument - Google Patents

Abstract

The utility model relates to a unicellular nucleic acid processing technology field discloses a unicellular nucleic acid processing apparatus, include: a frame; the air pump assembly is arranged on the rack; the box body structure comprises a box body driving assembly and a box body assembly, wherein the box body driving assembly can drive the box body assembly to move along the horizontal direction, and a placing groove is formed in the box body assembly; the box cover structure comprises a box cover driving assembly and a box cover assembly, the box cover driving assembly can drive the box cover assembly to move along the vertical direction so that the box cover assembly is covered on the box body assembly, the box cover assembly comprises a box cover body, an air inlet hole group and an air exhaust hole group are arranged on the box cover body, and the air inlet hole group and the air exhaust hole group are respectively communicated with the air pump assembly; the chip structure is arranged in the placing groove. The utility model discloses a unicellular nucleic acid processing apparatus simple structure, degree of automation is higher, and is shorter during the experiment, and experimental operation is simpler, and the customer uses the satisfaction higher.

Description

Single cell nucleic acid processing instrument

Technical Field

The utility model relates to a unicellular nucleic acid processing technology field especially relates to a unicellular nucleic acid processing apparatus.

Background

The single-cell nucleic acid processing instrument is used for extracting DNA or RNA in cells, and the existing single-cell nucleic acid processing instrument has the defects of complex structure and low automation degree, so that the problems of complex operation and long experimental time exist in actual operation, and the using satisfaction of customers is reduced.

SUMMERY OF THE UTILITY MODEL

Based on above, the utility model aims to provide a unicellular nucleic acid processing apparatus has simple structure and the high advantage of degree of automation.

In order to achieve the purpose, the utility model adopts the following technical proposal:

a single-cell nucleic acid processing instrument, comprising: a frame; the air pump assembly is arranged on the rack; the box body structure comprises a box body driving assembly and a box body assembly, wherein the box body driving assembly is arranged on the rack, the box body assembly is arranged at the output end of the box body driving assembly, the box body driving assembly can drive the box body assembly to move along the horizontal direction, and a placing groove is formed in the box body assembly; the box cover structure comprises a box cover driving assembly and a box cover assembly, the box cover driving assembly is arranged on the rack, the box cover assembly is arranged at the output end of the box cover driving assembly and is positioned above the box body assembly, the box cover driving assembly can drive the box cover assembly to move along the vertical direction so as to enable the box cover assembly to cover the box body assembly, the box cover assembly comprises a box cover body, an air inlet hole group and an air exhaust hole group are arranged on the box cover body, and the air inlet hole group and the air exhaust hole group are both communicated with the air pump assembly; the chip structure is arranged in the placing groove, the chip structure is provided with a sample adding groove, a sample groove, a waste liquid groove and a micro channel, the sample adding groove is communicated with the air inlet hole group, and the sample groove and the waste liquid groove are communicated with the air exhaust hole group.

As a preferable scheme of the single cell nucleic acid processing apparatus, the box cover assembly includes a first air inlet control valve, the air inlet group includes at least two air inlets, one first air inlet control valve is disposed at each air inlet, the first air inlet control valve is configured to control the connection or disconnection between the air inlets and the air pump assembly, an air inlet channel is further disposed on the box cover body, one end of the air inlet channel is respectively connected with at least two air inlets, and the other end of the air inlet channel is connected with the air pump assembly; the lid subassembly includes first air exhaust control valve and second air exhaust control valve, the air exhaust hole group includes two aspirating holes, two the aspirating hole is first aspirating hole and second aspirating hole respectively, first aspirating hole with waste liquid groove intercommunication just is equipped with first air exhaust control valve, the second aspirating hole with sample groove intercommunication just is equipped with the second air exhaust control valve, first air exhaust control valve with the second air exhaust control valve is configured to control the aspirating hole with the intercommunication or the disconnection of air pump subassembly, still be equipped with air exhaust passage on the lid body, air exhaust passage's one end and two the aspirating hole intercommunication, air exhaust passage's the other end with the air pump subassembly intercommunication.

As a preferred scheme of the single-cell nucleic acid processing apparatus, the single-cell nucleic acid processing apparatus further comprises a gas circuit integrated block, a first connecting channel, a second connecting channel and a first detection channel are arranged on the gas circuit integrated block, one end of the first connecting channel is communicated with the air pump assembly, the other end of the first connecting channel is communicated with the same end of the second connecting channel and the first detection channel, the other end of the second connecting channel is communicated with the air inlet channel, and the other end of the first detection channel can be communicated with a pressure detector; the gas circuit integrated block is also provided with a third connecting channel, a fourth connecting channel and a second detection channel, one end of the third connecting channel is communicated with the air pumping channel, the other end of the third connecting channel is communicated with the fourth connecting channel and the same end of the second detection channel, the other end of the fourth connecting channel is communicated with the air pump assembly, and the other end of the second detection channel can be communicated with the pressure detector.

As a preferable scheme of the single-cell nucleic acid processing instrument, the other end of the first detection channel is assembled with a first plug in an interference fit mode, and the other end of the second detection channel is assembled with a second plug in an interference fit mode.

As a preferable mode of the single-cell nucleic acid processing apparatus, the air pump module includes: the air pump motor is arranged on the rack; the air pump ball screw comprises an air pump screw and an air pump nut, the air pump screw is arranged at the output end of the air pump motor, and the air pump nut is in threaded connection with the air pump screw; the air pump body, including air pump section of thick bamboo and air pump push rod subassembly, the air pump section of thick bamboo sets up in the frame and inject the pump chamber of giving vent to anger in it, the pump chamber can respectively with the inlet opening group with bleed hole group intercommunication, the one end of air pump push rod subassembly with the sealed sliding connection of air pump section of thick bamboo, the other end of air pump push rod subassembly with air pump nut fixed connection.

As a preferred scheme of the instrument for processing single cell nucleic acid, the lid driving assembly includes two lid motor assemblies, two lid motor assemblies are respectively located on the left and right sides of the lid body, and each lid motor assembly includes: the box cover motor body is arranged on the rack; the box cover ball screw comprises a box cover screw rod and a box cover nut, the box cover screw rod is arranged at the output end of the box cover motor body, the box cover nut is in threaded connection with the box cover screw rod, and the box cover nut is fixedly connected with the box cover body.

As a preferable mode of the single-cell nucleic acid processing instrument, the rack includes a placing plate, the cassette motor body is disposed at a lower side of the placing plate, the cassette driving assembly further includes two cassette conveying assemblies disposed at an upper side of the placing plate, each of the cassette conveying assemblies includes: the first box cover gear is fixedly arranged at the output end of the box cover motor body; the second box cover gear is fixedly connected with the box cover screw rod; and the box cover conveying belt is meshed with the first box cover gear and the second box cover gear.

As a preferable mode of the single-cell nucleic acid processing apparatus, the cartridge driving unit includes: the first box body motor is arranged on the rack; box body ball, including box body lead screw and box body nut, the box body lead screw sets up the output of first box body motor, box body nut threaded connection be in on the box body lead screw and with box body subassembly fixed connection, first box body motor can drive the box body lead screw rotates so that the box body nut drives the box body subassembly is followed the horizontal direction motion.

As a preferable mode of the single-cell nucleic acid processing apparatus, the cartridge driving unit includes: the second box body motor is arranged on the rack; box body gear drive subassembly, including engaged box body gear and box body rack, the box body gear is fixed to be set up the output of second box body motor, the box body rack slides and sets up in the frame and with box body subassembly fixed connection, second box body motor can drive box body gear revolve so that the box body rack drives the box body subassembly is followed the horizontal direction motion.

As an optimal selection scheme of unicellular nucleic acid treatment instrument, the box body subassembly includes box body, heat preservation frame and heat-conducting component, heat-conducting component with the box body encloses into the open cavity in upper end, heat-conducting component is including overlapping heat-conducting piece, peltier and the radiating piece of establishing in proper order, peltier is located the below of heat-conducting piece just can heat and cool off the cavity, the radiating piece is located peltier's below, the heat preservation frame is followed heat-conducting piece's circumference sets up just the heat preservation frame presss from both sides and locates heat-conducting piece with between the radiating piece, be equipped with on the heat preservation frame and dodge the hole, it is just right to dodge the hole heat-conducting piece with the radiating piece sets up.

As a preferred scheme of the single cell nucleic acid processing instrument, the heat dissipation member is a heat dissipation fin, and the heat dissipation fin is arranged on the box body component; or: the heat dissipation piece is a heat dissipation plate, a cooling channel is arranged in the heat dissipation plate, and cooling water in the cooling channel can cool the heat dissipation plate.

As an optimal selection scheme of unicellular nucleic acid processing apparatus, unicellular nucleic acid processing apparatus still includes magnet feeding structure, magnet feeding structure includes magnet drive assembly, coupling assembling and magnet arm, magnet drive assembly sets up in the frame, coupling assembling sets up magnet drive assembly's output, coupling assembling includes first connecting block, second connecting block and feeds the elastic component, second connecting block slidable ground sets up on the first connecting block, it locates to feed the elastic component clamp first connecting block with between the second connecting block, can adsorb when first connecting block circular telegram the second connecting block, and when the outage the feed elastic component can reset the second connecting block, the magnet arm is fixed to be set up on the second connecting block, magnet drive assembly can drive coupling assembling drives the magnet arm is just right along the horizontal direction motion so that the magnet arm is just right the second connecting block And when the first connecting block is electrified, the magnet arm can move along with the second connecting block along the direction close to the chip structure along the vertical direction.

As a preferred scheme of the single-cell nucleic acid processing instrument, the chip structure comprises a chip box and at least one chip assembly, wherein the at least one chip assembly is arranged in the chip box, and the chip box is clamped in the placing groove.

The utility model has the advantages that: the utility model discloses a unicellular nucleic acid processing apparatus simple structure, box body drive assembly can drive the box body subassembly and move along the horizontal direction to make the box body subassembly be located the lid body under or outwards release the box body subassembly, lid drive assembly can drive the lid subassembly and move along vertical direction so that lid body cover establishes on the box body subassembly, degree of automation is higher, and is shorter during the experiment, and experimental operation is simpler, and customer's use satisfaction is higher.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required to be used in the description of the embodiments of the present invention will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the contents of the embodiments of the present invention and the drawings without creative efforts.

FIG. 1 is a schematic view of a single-cell nucleic acid processing apparatus according to an embodiment of the present invention in one direction;

FIG. 2 is a schematic view of a single-cell nucleic acid processing apparatus according to another embodiment of the present invention;

fig. 3 is a schematic diagram of a chip structure, a box assembly, and a part of a rack according to an embodiment of the present invention;

FIG. 4 is a cross-sectional view of a chip cartridge and a cartridge body assembly according to an embodiment of the present invention;

fig. 5 is a cross-sectional view of a cartridge assembly according to an embodiment of the present invention;

fig. 6 is a schematic diagram of a cartridge assembly according to an embodiment of the present invention;

fig. 7 is a schematic view of a box cover body provided in an embodiment of the present invention in one direction;

fig. 8 is a schematic view of the box cover body provided in another direction according to the embodiment of the present invention;

fig. 9 is a sectional view of the lid body along the direction a-a according to the embodiment of the present invention;

fig. 10 is a cross-sectional view of a lid assembly according to an embodiment of the present invention;

fig. 11 is a sectional view of the lid body along the direction B-B according to the embodiment of the present invention;

fig. 12 is a cross-sectional view of the lid body along the direction C-C according to the embodiment of the present invention;

fig. 13 is a schematic view of the gas circuit integrated block, the communicating pipe, the adjusting block, the adjusting member, the connecting joint, and the like provided in the embodiment of the present invention in a first direction;

fig. 14 is a schematic view of the gas circuit integrated block, the communicating pipe, the adjusting block, the adjusting member, and the like provided in the embodiment of the present invention in a second direction;

fig. 15 is a schematic view of the gas circuit integrated block, the communicating pipe, the adjusting block, the adjusting member, and the like provided in the embodiment of the present invention in a third direction;

fig. 16 is a cross-sectional view of a magnet feed structure according to an embodiment of the present invention;

fig. 17 is a schematic view of a magnet feeding structure according to an embodiment of the present invention.

In the figure:

1. a frame; 11. mounting a plate; 12. placing the plate;

21. an air pump body; 211. an air pump cylinder; 212. an air pump push rod assembly; 22. an air pump delivery assembly; 221. a first air pump gear; 222. a second air pump gear; 223. an air pump conveyor belt;

30. a placement groove; 31. a cartridge body drive assembly; 311. a first cartridge motor; 312. a ball screw of the box body; 32. a cartridge assembly; 320. a second card slot; 321. a box body; 322. a heat preservation frame; 3220. avoiding holes; 323. a heat conducting component; 3231. a heat conductive member; 3232. a heat sink; 324. clamping the guide post; 325. clamping the elastic piece; 326. a clamping block;

41. a box cover driving component; 411. a box cover motor body; 412. a box cover conveying assembly; 4121. a first lid gear; 4122. a second lid gear; 4123. a box cover conveyor belt; 42. a box cover assembly; 421. a box cover body; 42101. an air intake passage; 42102. an air inlet; 42103. an air extraction channel; 421041, a first extraction hole; 4210411, a first pumping communication hole; 4210412, a first blind connecting hole; 421042, a second extraction hole; 4210421, a second pumping communication hole; 4210422, a second connecting blind hole; 42105. a blind air inlet hole; 42106. a first detection hole; 42107. a second detection hole; 42108. air exhaust blind holes; 42109. a third detection hole; 421010, detecting blind holes; 421011, a fourth detection hole; 422. a first intake control valve; 423. a first air extraction control valve; 424. a second air extraction control valve; 425. a third air extraction control valve; 426. detecting the control valve; 427. a circuit board;

5. a chip structure; 501. a sample adding slot; 502. a sample tank; 503. a waste liquid tank; 51. a chip cartridge; 510. a first card slot; 511. clamping convex; 52. a chip assembly;

61. the gas circuit integrated block; 6101. a first connecting channel; 6102. a second connecting channel; 6103. a first detection channel; 6104. a third connecting channel; 6105. a fourth connecting channel; 6106. a second detection channel; 62. connecting a joint; 63. a communicating pipe; 64. an adjusting block; 65. an adjustment member;

71. a magnet drive assembly; 711. a feed motor; 712. a feed ball screw; 72. a connecting assembly; 721. a first connection block; 722. a second connecting block; 723. feeding an elastic member; 724. a feed guide post; 73. a magnet arm; 730. avoiding the groove.

Detailed Description

In order to make the technical problems, technical solutions and technical effects achieved by the present invention more clear, the embodiments of the present invention will be described in further detail with reference to the accompanying drawings, and obviously, the described embodiments are only some embodiments, not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in fig. 1 to 17, this embodiment provides a unicellular nucleic acid processing apparatus, which includes a frame 11, an air pump assembly, a box structure, a box cover structure and a chip structure 5, wherein the air pump assembly is disposed on the frame 11, the box structure includes a box driving assembly 31 and a box assembly 32, the box driving assembly 31 is disposed on the frame 11, the box assembly 32 is disposed at an output end of the box driving assembly 31, the box driving assembly 31 can drive the box assembly 32 to move along a horizontal direction, a placement groove 30 is disposed on the box assembly 32, the box cover structure includes a box cover driving assembly 41 and a box cover assembly 42, the box cover driving assembly 41 is disposed on the frame 11, the box cover assembly 42 is disposed at an output end of the box cover driving assembly 41 and located above the box assembly 32, the box cover driving assembly 41 can drive the box cover assembly 42 to move along a vertical direction so that the box cover assembly 42 covers the box assembly 32, lid subassembly 42 includes lid body 421, is equipped with inlet port group and bleed hole group on lid body 421, and inlet port group and bleed hole group all communicate with air pump assembly, and chip structure 5 sets up in standing groove 30, is equipped with application of sample groove 501, sample groove 502, waste liquid groove 503 and microchannel on chip structure 5, and application of sample groove 501 and inlet port group intercommunication, sample groove 502 and waste liquid groove 503 all communicate with bleed hole group.

The unicellular nucleic acid processing instrument that this embodiment provided simple structure, box body drive assembly 31 can drive box body subassembly 32 and follow the horizontal direction motion, so that box body subassembly 32 is located lid body 421 under or outwards release box body subassembly 32, lid drive assembly 41 can drive lid subassembly 42 and follow vertical direction motion so that lid body 421 covers and establish on box body subassembly 32, degree of automation is higher, the experiment is for a long time shorter, the experiment operation is simpler, customer usage satisfaction is higher.

Specifically, as shown in fig. 3, the chip structure 5 of the present embodiment includes a chip box 51 and two chip components 52, wherein the two chip components 52 are both disposed in the chip box 51, and the chip box 51 is clamped in the placing groove 30. In other embodiments, the number of the chip components 52 included in the chip structure 5 is not limited to two in this embodiment, and may be one, three, or more than three, and is specifically set according to actual needs. Each chip assembly 52 of the present embodiment includes a reagent cartridge, a chip body, and a sealing gasket, wherein the sealing gasket and the chip body are respectively bonded to two opposite sides of the reagent cartridge.

Specifically, as shown in fig. 4, one end of the chip box 51 is provided with a first engaging groove 510, the box assembly 32 is provided with an engaging guiding post 324, the engaging guiding post 324 is sleeved with an engaging elastic member 325 and a latch 326, one end of the engaging elastic member 325 is connected to the box assembly 32, the other end is connected to the latch 326, the latch 326 is slidably mounted on the engaging guiding post 324, and the latch 326 can be engaged with the engaging groove to fix the chip box 51 in the placing groove 30.

In order to better position the chip box 51, as shown in fig. 4, a locking protrusion 511 is further disposed at the other end of the chip box 51, and a second locking groove 320 corresponding to the locking protrusion 511 is disposed on the box assembly 32. During installation, at first, the protruding 511 joint of card of chip box 51 is in second draw-in groove 320, make the one end and the box body subassembly 32 joint of chip box 51, use external force to promote fixture block 326 in order to compress joint elastic component 325 after that, make the other end of chip box 51 place in standing groove 30, loosen fixture block 326 at last, fixture block 326 promotes fixture block 326 under the effect of joint elastic component 325, fixture block 326 joint is in first draw-in groove 510, namely the other end and the box body subassembly 32 joint of chip box 51, this kind of mounting means can prevent that chip box 51 from taking place the skew and leading to the application of sample groove 501 unable and intake vent group to communicate in the experimentation, sample groove 502 and waste liquid groove respectively unable and exhaust vent group to communicate, increase the success rate of experiment.

It should be noted that, as shown in fig. 3, the reagent kit of each chip assembly 52 of the present embodiment is provided with five sample adding slots 501, one sample slot 502 and one waste liquid slot 503, the gasket is provided with seven through holes, and the seven through holes are respectively arranged corresponding to the five sample adding slots 501, one sample slot 502 and one waste liquid slot 503 on the reagent kit. The chip body is provided with a micro-channel which is respectively communicated with five sample adding grooves 501, a sample groove 502 and a waste liquid groove 503, and the bottom of the micro-channel is provided with a pit. Correspondingly, two air inlet hole groups and two air outlet hole groups are arranged on the box cover body 421, each air inlet hole group comprises five air inlet holes 42102, the five air inlet holes 42102 are respectively arranged in one-to-one correspondence with the five sample adding grooves 501 of one chip structure 5, each air outlet hole group comprises two air outlet holes, one air outlet hole of each air outlet hole group is communicated with the sample groove 502 of the chip structure 5, and the other air outlet hole is communicated with the waste liquid groove 503 of the chip structure 5.

In other embodiments, the number of the sample adding grooves 501 on each chip assembly 52 is not limited to five in this embodiment, and may be other numbers, specifically set according to actual needs.

Specifically, as shown in fig. 6 and 10, the lid assembly 42 of the present embodiment further includes a first air intake control valve 422, a second air intake control valve (not shown in the drawings), a first air suction control valve 423, a second air suction control valve 424, a third air suction control valve 425, and a detection control valve 426, and the first air intake control valve 422, the second air intake control valve, the first air suction control valve 423, the second air suction control valve 424, the third air suction control valve 425, and the detection control valve 426 are provided on the lid body 421.

In the present embodiment, each air inlet hole 42102 is provided with a first air inlet control valve 422, the first air inlet control valve 422 is configured to control the connection or disconnection between the air inlet hole 42102 and the air pump assembly, as shown in fig. 9 and 12, the box cover body 421 is further provided with two air inlet channels 42101, the two air inlet channels 42101 and the two air inlet hole groups are arranged in a one-to-one correspondence manner, one end of each air inlet channel 42101 is respectively communicated with five air inlet holes 42102 of one air inlet hole group, and the other end is communicated with the air pump assembly. The inlet channel 42101 of this embodiment, inlet port group and bleed port group are integrated on lid body 421, compared with the prior art, inlet tubule and bleed tubule among the prior art have been saved, the inlet port group on lid body 421 communicates with sample adding groove 501 on chip structure 5, the first air inlet control valve 422 that sets up in inlet port 42102 department can control the intercommunication or the disconnection of inlet port 42102 and air pump assembly, bleed port group and waste liquid groove 503 and the receipts appearance groove on chip structure 5 communicate, the first air exhaust control valve 423 and the second air exhaust control valve 424 that set up at bleed port department can control the intercommunication or the disconnection of bleed port and air pump assembly, this unicellular nucleic acid processing apparatus's simple structure and gas circuit integration degree are high, be favorable to the modularization setting of unicellular nucleic acid processing apparatus.

Further, each air inlet hole 42102 of the present embodiment includes a first air inlet hole 42102 and a second air inlet hole 42102, the diameter of the second air inlet hole 42102 is smaller than the diameter of the first air inlet hole 42102, one end of the first air inlet control valve 422 extends into the first air inlet hole 42102 and forms a first upper air inlet cavity, a first middle air inlet cavity and a first bottom air inlet cavity with the air inlet hole 42102, the first middle air inlet cavity is located between the first upper air inlet cavity and the first bottom air inlet cavity, the air inlet channel 42101 is communicated with the first middle air inlet cavity, and the second air inlet hole 42102 is communicated with the first bottom air inlet cavity. When the first air inlet control valve 422 is powered off, the first upper air inlet cavity is communicated with the first middle air inlet cavity, and the gas entering the first middle air inlet cavity through the air inlet channel 42101 can enter the first bottom air inlet cavity and finally enter the sample adding slot 501 through the second air inlet hole 42102, so that the reagent in the sample adding slot 501 is introduced into the micro-channel.

As shown in fig. 11, the box cover body 421 of this embodiment is provided with two air inlet blind holes 42105 and two first detection holes 42106, each air inlet blind hole 42105 is provided with a second air inlet control valve (not shown in the figure), one end of the second air inlet control valve extends into the air inlet blind hole 42105 and forms a second upper air inlet cavity, a second middle air inlet cavity and a second bottom air inlet cavity with the air inlet blind hole 42105, the second middle air inlet cavity is located between the second upper air inlet cavity and the second bottom air inlet cavity, each first detection hole 42106 is communicated with one second upper air inlet cavity, the second middle air inlet cavity is communicated with the air pump assembly, the second bottom air inlet cavity is communicated with the air inlet passage 42101, the second air inlet control valve is configured to communicate the second upper air inlet cavity with the second middle air inlet cavity when the power is off, and communicate the second middle air inlet cavity with the second bottom air inlet cavity when the power is on.

Specifically, when the second air inlet control valve is powered off, the second upper air inlet cavity is communicated with the second middle air inlet cavity, and at this time, the pressure of the gas entering the second middle air inlet cavity by the air pump assembly can be detected through the first detection hole 42106; when the second air inlet control valve is powered on, the second middle air inlet cavity is communicated with the second bottom air inlet cavity, at the moment, air discharged by the air pump assembly enters the second bottom air inlet cavity through the second middle air inlet cavity, and air in the second bottom air inlet cavity enters the first middle air inlet cavity through the air inlet channel 42101.

As shown in fig. 9, 11 and 12, the two air exhaust holes of each air exhaust hole group of the present embodiment are a first air exhaust hole 421041 and a second air exhaust hole 421042, respectively, the first air exhaust hole 421041 includes a first air exhaust communication hole 4210411 and a first connection blind hole 4210412, the first air exhaust communication hole 4210411 communicates with the waste liquid tank 503, the second air exhaust hole 421042 includes a second air exhaust communication hole 4210421 and a second connection blind hole 4210422, the second air exhaust communication hole 4210421 communicates with the sample receiving tank, a first air exhaust control valve 423 is provided in the first connection blind hole 4210412, the first air exhaust control valve 423 and the first connection blind hole 4210412 form a first upper connection chamber, a first middle connection chamber and a first bottom connection chamber, a second detection hole 42107 is provided on the box cover body 421, the second detection hole 42107 communicates with the first bottom connection chamber, the first middle connection chamber communicates with the first air exhaust control valve 4210411, the first air exhaust control valve 423 is configured to communicate the first upper connection chamber with the first middle connection chamber when power is off, when the power is on, the first middle connecting cavity is communicated with the first bottom connecting cavity. Be provided with second control valve 424 of taking out in the second connection blind hole 4210422, second control valve 424 of taking out forms second upper portion with second connection blind hole 4210422 and connects the chamber, chamber and second bottom connection chamber are connected at the second middle part, the chamber is connected to the second middle part and is located second upper portion and connect between the chamber and the second bottom connection chamber, second upper portion connection chamber and second intercommunicating pore 4210421 intercommunication of taking out, chamber and air exhaust passage 42103 intercommunication are connected at the second middle part, chamber and first upper portion connection chamber intercommunication are connected to the second bottom connection chamber, control valve 424 is taken out to the second when being configured as the outage with second upper portion connection chamber and second middle part connection chamber intercommunication, connect chamber and second bottom connection chamber intercommunication with the second middle part when the circular telegram.

Specifically, when the first air pumping control valve 423 is powered off and the second air pumping control valve 424 is powered on, the first upper connecting cavity is communicated with the first middle connecting cavity and the first middle connecting cavity is communicated with the first air pumping communication hole 4210411, which is equivalent to pumping air to the waste liquid tank 503, because the second air pumping control valve 424 is powered on, the second middle connecting cavity is communicated with the second bottom connecting cavity and the air pumping channel 42103 is communicated with the second middle connecting cavity, because the second bottom connecting cavity is communicated with the first upper connecting cavity, the air pump assembly can pump the air in the waste liquid tank 503 to the air pumping channel 42103 through the first air pumping communication hole 4210411, the first middle connecting cavity, the first upper connecting cavity, the second bottom connecting cavity and the second middle connecting cavity in sequence.

When the first evacuation control valve 423 is energized, the second bottom connection chamber is not communicated with the first upper connection chamber, that is, the air pump assembly cannot continue to evacuate the gas in the waste liquid tank 503, so that the first middle connection chamber is communicated with the first bottom connection chamber, the first middle connection chamber is communicated with the first evacuation communication hole 4210411, the first evacuation communication hole 4210411 is communicated with the waste liquid tank 503, and the second detection hole 42107 is communicated with the first bottom connection chamber, and therefore, the pressure of the gas in the waste liquid tank 503 can be detected through the second detection hole 42107.

When the second air pumping control valve 424 is powered off, the second upper connecting cavity is communicated with the second middle connecting cavity, the air pumping channel 42103 is communicated with the second middle connecting cavity, the second upper connecting cavity is communicated with the second air pumping communication hole 4210421, and the second middle connecting cavity is not communicated with the second bottom connecting cavity, so that the air pump assembly cannot pump the gas in the waste liquid tank 503, and at the moment, the air pump assembly can pump the gas in the sample collection tank to the air pumping channel 42103 through the second air pumping communication hole 4210421, the second upper connecting cavity and the second middle connecting cavity in sequence.

As shown in fig. 7, 9, 11 and 12, the box cover body 421 of this embodiment is provided with two air exhaust blind holes 42108 and two third detection holes 42109, each air exhaust blind hole 42108 is provided with a third air exhaust control valve 425, one end of the third air exhaust control valve 425 extends into the air exhaust blind hole 42108 and forms an upper air exhaust cavity, a middle air exhaust cavity and a bottom air exhaust cavity with the air exhaust blind hole 42108, the middle air exhaust cavity is located between the upper air exhaust cavity and the bottom air exhaust cavity, one third detection hole 42109 is communicated with one upper air exhaust cavity, the middle air exhaust cavity is communicated with the air pump assembly, the bottom air exhaust cavity is communicated with the air exhaust passage 42103, the third air exhaust control valve 425 is configured to communicate the upper air exhaust cavity and the middle air exhaust cavity when power is off, and communicate the middle air exhaust cavity with the bottom air exhaust cavity when power is on.

Specifically, when the third pumping control valve 425 is powered off, the upper pumping cavity is communicated with the middle pumping cavity, the air pump assembly is communicated with the upper pumping cavity through the middle pumping cavity, and at this time, the pressure of the gas in the upper pumping cavity can be detected through the third detection hole 42109; when the third pumping control valve 425 is energized, the middle pumping cavity is communicated with the bottom pumping cavity, and at this time, the air pump assembly pumps air in the pumping channel 42103 through the middle pumping cavity and the bottom pumping cavity.

As shown in fig. 6, 7, 9, 11 and 12, the box cover body 421 of this embodiment is further provided with two detection blind holes 421010 and two fourth detection holes 421011, each air inlet passage 42101 corresponds to one detection blind hole 421010 and one fourth detection hole 421011, as shown in fig. 6, a detection control valve 426 is disposed at each detection blind hole 421010, each detection control valve 426 and the detection blind hole 421010 form a middle detection chamber and a bottom detection chamber, the bottom detection chamber is communicated with the fourth detection hole 421011, the middle detection chamber is communicated with the air inlet passage 42101, and the detection control valve 426 is configured to control the communication or disconnection between the middle detection chamber and the bottom detection chamber.

Specifically, when the detection control valve 426 is opened, the middle detection chamber and the bottom detection chamber are communicated, and the pressure of the gas in the bottom detection chamber can be detected through the fourth detection hole 421011; when the detection control valve 426 is closed, the middle detection chamber is not communicated with the bottom detection chamber, and the pressure of the gas in the bottom detection chamber cannot be detected through the fourth detection hole 421011.

As shown in fig. 6 and 10, the lid assembly 42 of the present embodiment further includes a circuit board 427, and the circuit board 427 is electrically connected to the ten first air intake control valves 422, the two second air intake control valves, the two first air suction control valves 423, the two second air suction control valves 424, the two third air suction control valves 425, and the two detection control valves 426, respectively.

When the single-cell nucleic acid processing instrument of the embodiment is used for introducing gas into the sample adding slot 501, the first gas inlet control valve 422 is powered on, the second gas inlet control valve is powered on, gas discharged by the gas pump assembly enters the second bottom gas inlet cavity through the second middle gas inlet cavity, gas in the second bottom gas inlet cavity enters the first middle gas inlet cavity through the gas inlet channel 42101, gas in the first middle gas inlet cavity can enter the first bottom gas inlet cavity, and finally enters the sample adding slot 501 through the second gas inlet hole 42102, so that a reagent in the sample adding slot 501 enters the micro-channel.

When the single-cell nucleic acid processing apparatus of this embodiment is used to pump gas from the waste liquid tank 503, the first pumping control valve 423 is turned off and the second pumping control valve 424 is turned on, and the air pump assembly can pump gas from the waste liquid tank 503 to the pumping passage 42103 through the first pumping communication hole 4210411, the first middle connection chamber, the first upper connection chamber, the second bottom connection chamber, and the second middle connection chamber in this order.

When the single-cell nucleic acid processing apparatus of this embodiment is used to pump gas in the sample collection tank, the second pumping control valve 424 is turned off, and the air pump assembly can pump gas in the sample collection tank to the pumping channel 42103 through the second pumping communication hole 4210421, the second upper connecting chamber, and the second middle connecting chamber in this order.

As shown in fig. 12 to 15, the single-cell nucleic acid processing apparatus of the present embodiment further includes an air circuit integrated block 61, two first connecting channels 6101, two second connecting channels 6102, and two first detecting channels 6103 are disposed on the air circuit integrated block 61, one end of each first connecting channel 6101 is communicated with the air pump assembly, the other end of the first connecting channel 6101 is communicated with the same end of one second connecting channel 6102 and one first detecting channel 6103, the other end of the second connecting channel 6102 is communicated with one air inlet channel 42101 through a connecting joint 62 and a communicating tube 63, and the other end of the first detecting channel 6103 is communicated with the first pressure detector. The air path manifold 61 is further provided with two third connecting channels 6104, two fourth connecting channels 6105 and two second detecting channels 6106, one end of each third connecting channel 6104 is communicated with the air pumping channel 42103 through the connecting joint 62 and the communicating pipe 63, the other end of the third connecting channel 6104 is communicated with the same end of one fourth connecting channel 6105 and one second detecting channel 6106, the other end of the fourth connecting channel 6105 is communicated with the air pump assembly, and the other end of the second detecting channel 6106 is communicated with the second pressure detector.

As shown in FIGS. 12 and 15, the single-cell nucleic acid processing apparatus of the present embodiment further comprises an adjustment block 64 and an adjustment member 65, wherein the adjustment block 64 is fixed to the manifold block, the connection pipe 63 is disposed to penetrate the adjustment block 64, the adjustment member 65 is disposed on the adjustment block 64 and can be abutted to the connection pipe 63, the adjustment member 65 is a bolt, and the flow rate of the gas flowing through the connection pipe 63 per unit time can be changed by screwing the adjustment bolt into the adjustment block 64.

In other embodiments, the other end of the first detection channel 6103 is not provided with a first pressure detector, but is interference fitted with a first plug, and the other end of the second detection channel 6106 is not provided with a second pressure detector, but is interference fitted with a second plug. When the pressure in the first connecting channel 6101 and the second connecting channel 6102 needs to be detected, the first plug is pulled down, and the external pressure detector is communicated with the first detecting channel 6103; when the pressure in the third connecting channel 6104 and the fourth connecting channel 6105 needs to be detected, the second plug is pulled down, so that the external pressure detector is communicated with the second detecting channel 6106.

In the prior art, in order to detect the gas pressure entering the two gas inlet channels 42101, a gas inlet three-way pipe is arranged between each gas inlet channel 42101 and the gas pump assembly, in order to detect the gas pressure entering the two gas exhaust channels 42103, a gas exhaust three-way pipe is arranged between each gas exhaust channel 42103 and the gas pump assembly, the gas path manifold block 61 of the embodiment replaces the two existing gas inlet three-way pipes and the two existing gas exhaust three-way pipes, and the structure is simpler and more compact.

As shown in fig. 1, the number of the air pump assemblies of this embodiment is two, each air pump assembly includes an air pump motor, an air pump ball screw, an air pump body 21 and an air pump transmission assembly 22, the air pump motor is disposed on the frame 11, the air pump transmission assembly 22 includes a first air pump gear 221, a second air pump gear 222 and an air pump transmission belt 223, the first air pump gear 221 is disposed at an output end of the air pump motor, the air pump transmission belt 223 is disposed on the first air pump gear 221 and the second air pump gear 222, the air pump motor can drive the first air pump gear 221 to rotate so as to enable the second air pump gear 222 to rotate synchronously therewith, the air pump ball screw includes an air pump screw and an air pump nut, the air pump screw is fixedly connected to the second air pump gear 222, the air pump nut is connected to the air pump screw in a threaded manner, the air pump body 21 includes an air pump cylinder 211 and an air pump push rod assembly 212, the air pump cylinder 211 is disposed on the frame 11 and defines an air outlet pump chamber therein, the air pump cavity can be respectively communicated with the air inlet hole group and the air exhaust hole group, one end of the air pump push rod assembly 212 is in sealing sliding connection with the air pump barrel 211, and the other end of the air pump push rod assembly is fixedly connected with the air pump nut. Specifically, the air pump push rod assembly 212 includes a push rod body and a piston, one end of the push rod body is fixedly connected with the air pump nut, the other end of the push rod body is fixedly connected with the piston, and the piston is in sealing sliding connection with the air pump cylinder 211.

As shown in FIG. 1, the rack 11 of the present embodiment comprises a mounting plate 11, the air pump motor is disposed at the lower side of the mounting plate 11, and the air pump transmission assembly 22 is disposed at the upper side of the mounting plate 11, so that the air pump assembly has a lower total height and a more compact structure, which is beneficial to the miniaturization of the single-cell nucleic acid processing instrument.

Because the air pump body 21 is controlled by the air pump ball screw, the movement precision is higher, and the precise micro-feeding of the air pump push rod assembly 212 is realized. When the reagent in the sample adding groove 501 needs to be added into the microchannel, the sample adding groove 501 is inflated, the air pump motor drives the air pump lead screw to rotate so as to enable the air pump nut to drive the air pump push rod assembly 212 to move along the vertical downward direction, and at the moment, the air in the air pump cylinder 211 is sequentially pushed into the first connecting channel 6101, the second connecting channel 6102, the air inlet channel 42101 and one of the air inlet holes 42102 and finally enters the sample adding groove 501, so that the reagent in the sample adding groove 501 is pushed to flow and enter the microchannel; when the waste liquid tank 503 needs to be pumped so that the reagents in the micro-channels enter the waste liquid tank 503, the air pump motor drives the air pump lead screw to rotate so that the air pump nut drives the air pump push rod assembly 212 to move along the vertical upward direction, at this time, the gas in the waste liquid tank 503 sequentially enters the air pump cylinder 211 through the pumping hole, the pumping channel 42103, the fourth connecting channel 6105 and the third connecting channel 6104, and when the sample tank 502 is pumped in the same manner, the pumping process is similar to the process of pumping the gas in the waste liquid tank 503.

As shown in fig. 1 and 2, the lid driving assembly 41 of this embodiment includes two lid motor assemblies, the two lid motor assemblies are respectively located on the left side and the right side of the lid body 421, each lid motor assembly includes a lid motor body 411 and a lid ball screw (not shown in the figure), the lid motor body 411 is disposed on the frame 11, the lid ball screw includes a lid screw and a lid nut, the lid screw is disposed at the output end of the lid motor body 411, the lid nut is screwed onto the lid screw, and the lid nut is fixedly connected to the lid body 421.

Specifically, as shown in fig. 1, the frame 11 of the embodiment includes a placing plate 12, a lid motor body 411 is disposed at a lower side of the placing plate 12, as shown in fig. 1, the lid driving assembly 41 further includes two lid conveying assemblies 412, the lid conveying assemblies 412 are disposed at an upper side of the placing plate 12, as shown in fig. 1, each lid conveying assembly 412 includes a first lid gear 4121, a second lid gear 4122, and a lid conveyor belt 4123, the first lid gear 4121 is fixedly disposed at an output end of the lid motor body 411, the second lid gear 4122 is fixedly connected to the lid screw, and the lid conveyor belt 4123 is engaged with the first lid gear 4121 and the second lid gear 4122. The lid motor body 411 is disposed at the lower side of the placing plate 12, and the lid transfer unit 412 is disposed at the upper side of the placing plate 12, so that the lid driving unit 41 has a lower total height and a more compact structure, and is advantageous for the miniaturization of the instrument for processing single-cell nucleic acid.

In other embodiments, if the height of the instrument is not limited, the lid driving unit 41 may not include the lid transferring unit 412, and the lid motor 411 is disposed on the upper side of the placing plate 12, and the lid screw is directly disposed at the output end of the lid motor 411.

The lid motor body 411 of the two lid motor assemblies of this embodiment can drive the lid ball screw simultaneously and drive the lid body 421 to move up or down along the vertical direction, so that the lid body 421 covers the box assembly 32. Among the prior art, in order to realize the motion of lid body 421 along vertical direction, two lid drive assembly 41 are two cylinder drive structures, when cylinder drive structure drive lid body 421, the displacement distance that often exists the both sides of lid body 421 is different, make lid body 421 unable and box body subassembly 32 lock completely, make to have the gap between the inlet port 42102 of inlet port group and the application of sample groove 501 on the chip structure 5, or there is the gap between the extraction opening of extraction opening group and the sample groove 502 and waste liquid groove 503 on the chip structure 5, be unfavorable for going on of whole experiment.

The lid drive assembly 41 of this embodiment compares with the motion of current cylinder drive slider drive lid body 421, and lid motor body 411 and lid ball can make lid body 421 follow vertical direction motion and have higher precision, realize the accurate microfeed to lid body 421 to make lid body 421 and box body subassembly 32 lock completely.

As shown in fig. 2, the box driving assembly 31 of this embodiment includes a first box motor 311 and a box ball screw 312, the first box motor 311 is disposed on the frame 11, the box ball screw 312 includes a box screw and a box nut, the box screw is disposed at an output end of the first box motor 311, the box nut is screwed on the box screw and is fixedly connected to the box assembly 32, and the first box motor 311 can drive the box screw to rotate so that the box nut drives the box assembly 32 to move along a horizontal direction. The first cassette motor 311 and the cassette ball screw 312 enable the cassette assembly 32 to move in the horizontal direction with high precision, and precise micro-feeding of the cassette assembly 32 is achieved.

In other embodiments, the box driving assembly 31 includes a second box motor and a box gear transmission assembly, the second box motor is disposed on the frame 11, the box gear transmission assembly includes a box gear and a box rack that are engaged with each other, the box gear is fixedly disposed at an output end of the second box motor, the box rack is slidably disposed on the frame 11 and fixedly connected to the box assembly 32, and the second box motor can drive the box gear to rotate so that the box rack drives the box assembly 32 to move along a horizontal direction.

As shown in fig. 5, the case assembly 32 of this embodiment includes a case body 321, a heat-insulating frame 322, and a heat-conducting assembly 323, the heat-conducting assembly 323 and the case body 321 enclose a cavity with an open upper end, as shown in fig. 5, the heat-conducting assembly 323 includes a heat-conducting element 3231, a peltier (not shown in the figure) and a heat-dissipating element 3232 stacked in sequence, a heat-dissipating fan (not shown in the figure) is disposed on the frame 11, the heat-conducting element 3231 is disposed on the case assembly 32 and encloses a cavity with an open upper end, the peltier is disposed below the heat-conducting element 3231 and can heat and cool the cavity, the peltier is disposed on one side of the heat-dissipating element 3232 close to the heat-conducting element 3231 or on one side of the heat-conducting element 3231 close to the heat-dissipating element 3232, the heat-insulating frame 3232 is disposed below the peltier, the heat-insulating frame 322 is disposed along the circumferential direction of the heat-conducting element 3231 and the heat-insulating frame 322 is disposed between the heat-conducting element 3231 and the heat-dissipating element 3232, the insulating frame 322 is provided with a relief hole 3220, the avoiding hole 3220 is disposed opposite to the heat conducting member 3231 and the heat dissipating member 3232, and the heat dissipating fan is fixedly disposed on the frame 11 and can be disposed opposite to the heat dissipating member 3232.

The heat conducting member 3231 of this embodiment is a copper block, which has a good heat conducting effect. Specifically, when the cavity is heated, heat is generated at one side of the Peltier close to the copper block, and the copper block can rapidly transfer the heat generated by the Peltier into the cavity, so that the cavity is heated; when cooling down the cavity, the one side refrigeration that the copper billet is close to the peltier, the copper billet can be with the quick transmission of the heat in the cavity to the peltier to cool down the cavity. The placement grooves 30 are provided on the copper block, and the placement grooves 30 of the present embodiment can place two chip structures 5 at the same time. In other embodiments, the heat conducting member 3231 is not limited to the copper block of this embodiment, the heat conducting member 3231 may be made of other materials with good heat conductivity, and the placing groove 30 on the heat conducting member 3231 may also be used to place one or at least three chip structures 5.

As shown in fig. 5, the heat sink 3232 of the present embodiment is a heat sink fin, and the peltier is disposed on a side of the heat sink fin close to the heat conducting member 3231. Specifically, when the cavity needs to be cooled, the peltier device is connected with the power supply in the positive direction, the temperature of one side, close to the cavity, of the peltier device is reduced, the heat conducting piece 3231 rapidly transfers the heat in the cavity to the peltier device, the temperature of one side, away from the cavity, of the peltier device is increased, and the heat radiating fins absorb the heat of the peltier device and cool the peltier device in time; when the chamber needs to be heated, the peltier device is connected with the power supply in the reverse direction, namely the direction of current is switched, at the moment, the temperature of the side, close to the chamber, of the peltier device is increased, the temperature of the side, away from the chamber, of the peltier device is reduced, and at the moment, the peltier device heats the chamber through the heat conducting piece 3231. In other embodiments, the mounting position of the peltier element is not limited to this limitation of the present embodiment, and may be provided on a side of the heat-conducting member 3231 close to the heat-radiating fins.

The case assembly 32 of this embodiment further includes a temperature sensor (not shown), a first temperature switch (not shown), and a second temperature switch (not shown), where the temperature sensor is used to measure the temperature of the heat-conducting member 3231, the first temperature switch and the second temperature switch are arranged in series, the first temperature switch is capable of measuring the temperature of the heat-radiating member 3232 and configured to be turned off when the temperature of the heat-radiating member 3232 reaches a first preset temperature, so as to turn off the peltier and the power supply, and stop to continue heating the chip structure 5, and the second temperature switch is capable of measuring the temperature of the heat-conducting member 3231 and configured to be turned off when the temperature of the heat-conducting member 3231 reaches a second preset temperature, so as to turn off the peltier and the power supply, and stop to continue heating the chip structure 5, where the second preset temperature is higher than the first preset temperature.

Specifically, when the chip structure 5 is heated, the peltier element is powered on, the heat conducting member 3231 can be heated after the peltier element is powered on, and when the temperature of the heat radiating member 3232 measured by the first temperature switch exceeds a first preset temperature, the first temperature switch is turned off to disconnect the peltier element from the power supply, and the chip structure 5 stops being continuously heated; or when the second temperature switch detects that the temperature of the thermal conductor 3231 reaches a second preset temperature, the second temperature switch is turned off to disconnect the peltier device from the power supply, and the chip structure 5 stops being heated.

When the temperature sensor cannot normally feed back the real-time temperature of the heat dissipation member 3232, the temperatures of the heat conduction member 3231 and the heat dissipation member 3232 are both continuously increased, and when the temperature of the heat dissipation member 3232 measured by the first temperature switch exceeds a first preset temperature or the temperature of the heat conduction member 3231 detected by the second temperature switch is higher than a second preset temperature, the peltier is disconnected from the power supply, and the chip structure 5 is stopped from being continuously heated.

In other embodiments, the heat dissipating member 3232 is a heat dissipating plate, a cooling channel is disposed in the heat dissipating plate, and cooling water in the cooling channel can cool the heat dissipating plate. Specifically, the water entering the water chiller is high-temperature water discharged from the heat dissipation plate, the water chiller can cool the high-temperature water to form low-temperature water, and the low-temperature water is discharged from an outlet of the water chiller and enters the heat dissipation plate again to absorb heat.

The single cell nucleic acid processing apparatus of the present embodiment further includes a magnet feeding structure, as shown in fig. 16 and 17, the magnet feeding structure includes a magnet driving assembly 71, a connecting assembly 72 and a magnet arm 73, the magnet driving assembly 71 is disposed on the frame 11, the connecting assembly 72 is disposed at an output end of the magnet driving assembly 71, as shown in fig. 16, the connecting assembly 72 includes a first connecting block 721, a second connecting block 722 and a feeding elastic member 723, the feeding elastic member 723 is a feeding spring, the second connecting block 722 is slidably disposed on the first connecting block 721, the feeding elastic member 723 is interposed between the first connecting block 721 and the second connecting block 722, the first connecting block 721 can attract the second connecting block 722 when the power is turned on, and the feeding elastic member 723 can reset the second connecting block 722 when the power is turned off, the magnet arm 73 is fixedly disposed on the second connecting block 722, the magnet driving assembly 71 can drive the connecting assembly 72 to drive the magnet arm 73 to move in a horizontal direction so that the magnet arm 73 faces the chip structure 5, the magnet arm 73 can move in the vertical direction toward the chip structure 5 with the second connection block 722 when the first connection block 721 is energized.

The magnet drive assembly 71 of the magnet feeding structure provided by this embodiment can drive the magnet arm 73 to move along the horizontal direction, so that the magnet arm 73 is just opposite to the chip structure 5, the first connecting block 721 can adsorb the second connecting block 722 after being powered on, so that the second connecting block 722 moves along the direction close to the chip structure 5 in the vertical direction, so that the distance from the magnet arm 73 to the chip structure 5 is closer, so that the magnet arm 73 can better adsorb magnetic beads with cells, the probability that the magnetic beads with cells are separated from pits at the bottom of a microchannel is increased, and the added feeding elastic member 723 can reset the second connecting block 722 when the first connecting block 721 is powered off.

Specifically, as shown in fig. 16, the connecting assembly 72 of the present embodiment further includes a feeding guide post 724 extending in the vertical direction, one end of the feeding guide post 724 extends into the first connecting block 721, the other end extends into the second connecting block 722, and the feeding elastic member 723 is sleeved on the feeding guide post 724. The additional feeding guide post 724 enables the first connecting block 721 to adsorb the second connecting block 722 to move the second connecting block 722 in the vertical direction when the first connecting block 721 is powered on, and enables the second connecting block 722 to move in the vertical direction when the feeding elastic member 723 resets the second connecting block 722 when the first connecting block 721 is powered off. Further, the number of the feeding guide posts 724 of the present embodiment is four, the four feeding guide posts 724 are respectively located at four corners of the first connecting block 721, the number of the feeding elastic members 723 is four, the feeding guide posts 724 and the feeding elastic members 723 are arranged in a one-to-one correspondence, and each feeding elastic member 723 is sleeved on one feeding guide post 724. In other embodiments, the number of the feeding guide posts 724 and the feeding elastic member 723 is not limited to four in this embodiment, and may be one, two, three or more than four, which is specifically selected according to actual needs.

Specifically, the first connecting block 721 of the present embodiment is an electromagnet, and the second connecting block 722 is an iron block. When the electromagnet is energized, the electromagnet can generate an electromagnet to attract the iron block so that the iron block drives the magnet arm 73 to move in the vertical direction, the magnet arm 73 moves towards the direction close to the chip structure 5, and the feeding elastic member 723 is compressed; when the electromagnet is powered off, the magnetism of the electromagnet disappears, the acting force between the electromagnet and the iron block disappears, and the feeding elastic member 723 pushes the second connecting block 722 to move in the vertical direction towards the direction away from the chip structure 5, so that the iron block is reset to the initial position. Further, when the first connecting block 721 of the present embodiment is energized, the second connecting block 722 can move 1mm in the vertical direction toward the direction close to the first connecting block 721, that is, the feeding elastic member 723 is compressed by 1 mm; when the second connecting block 722 is powered off, the feeding elastic member 723 can push the second connecting block 722 to reset. In other embodiments, the distance that the feeding elastic member 723 is compressed when the first connection block 721 is powered on is not limited to 1mm in this embodiment, but may be other distances, and is specifically set according to actual needs.

As shown in fig. 16 and 17, the magnet driving assembly 71 of the present embodiment includes a feeding motor 711 and a feeding ball screw 712, the feeding motor 711 is a rotating motor, an output end of the feeding motor 711 is connected to the feeding ball screw 712, a first connecting block 721 is disposed on the feeding ball screw 712, and the feeding motor 711 and the feeding ball screw 712 can move the magnet arm 73 in the horizontal direction with high precision, thereby realizing precise micro feeding of the magnet arm 73. Specifically, the feeding ball screw 712 includes a feeding screw rod and a feeding screw block, the feeding screw block is screwed on the feeding screw rod, the connecting assembly 72 is fixedly disposed on the feeding screw block, and the feeding motor 711 can drive the feeding screw rod to rotate so that the feeding screw block drives the connecting assembly 72 to move in the horizontal direction.

In order to ensure that the feeding screw block can linearly move along the horizontal direction without rotating relative to the frame 11, the frame 11 of this embodiment is provided with two feeding slide rails (not shown in the figure) extending along the horizontal direction, the feeding screw block is provided with two feeding slide grooves (not shown in the figure) corresponding to the feeding slide rails, and the feeding screw block is slidably connected to the frame 11.

The magnet arm 73 of this embodiment is provided with two avoidance slots 730, and the two avoidance slots 730 are arranged in parallel and the avoidance slot 730 penetrates through the magnet arm 73. The avoidance groove 730 additionally arranged can avoid the phenomenon that the magnet arm 73 extrudes the chip structure 5 to damage the chip structure 5, and the magnet arm 73 is still arranged at an interval with the chip structure 5 while moving towards the direction close to the chip structure 5 along the vertical direction.

Preferably, the single-cell nucleic acid processing apparatus of this embodiment further includes a controller, the controller is electrically connected to the air pump assembly, the box body driving assembly 31, the box cover driving assembly 41, the peltier, the first connection block 721 and the feeding motor 721, respectively, the controller may be a centralized or distributed controller, for example, the controller may be a single-chip microcomputer or may be formed by a plurality of distributed single-chip microcomputers, and the single-chip microcomputers may run control programs to control the air pump assembly, the box body driving assembly 31, the box cover driving assembly 41, the peltier, the first connection block 721 and the feeding motor 721 to implement their functions.

When the single-cell nucleic acid processing instrument of the embodiment is used for extracting RNA in cells, the specific operation steps are as follows:

step one, a box cover motor body 411 is started, the box cover motor body 411 drives a box cover ball screw and a box cover conveying assembly 412 to drive a box cover assembly 42 to move upwards along the vertical direction, and the box cover assembly 42 is separated from a box body assembly 32;

step two, two first box motors 311 are started simultaneously, and the first box motors 311 drive the box ball screws 312 to drive the box assembly 32 to extend outwards along the horizontal direction;

step three, the experimenter clamps the chip structure 5 on the box body assembly 32;

step four, adding a first reagent, a solution with cells, a solution with magnetic beads, a second reagent and a third reagent required by the experiment into the five sample adding grooves 501 of each chip assembly 52;

step five, the two first box motors 311 are started simultaneously, and the first box motors 311 drive the box ball screws 312 to drive the box assembly 32 to reset to the position right below the box cover assembly 42 along the horizontal direction;

step six, the box cover motor body 411 is started, the box cover motor body 411 drives the box cover ball screw and the box cover conveying assembly 412 to drive the box cover assembly 42 to move downwards along the vertical direction, and the box cover assembly 42 is attached to the box body assembly 32;

step seven, starting an air pump motor, electrifying a first air inlet control valve 422 for controlling the on-off of an air path of the sample adding groove 501 for placing the first reagent, enabling air to enter the sample adding groove 501 for placing the first reagent, pressing the first reagent into the chip body, and then powering off the first air inlet control valve 422 electrified in the step so as to stop the flow of the first reagent;

step eight, starting an air pump motor, controlling a first air pumping control valve 423 which is switched on and off by an air path of a waste liquid tank 503 into which the solution with cells is put to be electrified, so that the air pressure in the waste liquid tank 503 is changed, thereby driving the liquid in the micro-channel to flow, cleaning the micro-channel by a first reagent, and then switching off the first air pumping control valve 423 which is electrified in the step, thereby stopping the flow of the solution in the micro-channel;

step nine, electrifying a first air inlet control valve 422 for controlling the on-off of an air path of the sample adding groove 501 for placing the solution with cells, enabling the air to enter the sample adding groove 501 for placing the solution with cells, pressing the solution with cells into the chip body, and then powering off the first air inlet control valve 422 of the electromagnetic valve electrified in the step, so that the solution with cells stops flowing;

step ten, starting an air pump motor, controlling a first air pumping control valve 423 which is switched on and off by an air path of a waste liquid tank 503 into which the solution with cells is put to be electrified, changing the air pressure in the waste liquid tank 503 so as to drive the liquid in the micro-channel to flow, pumping the solution with cells into the micro-channel, and then switching off the first air pumping control valve 423 which is electrified in the step so as to stop the flow of the solution in the micro-channel;

step eleven, allowing a certain time for the cells to settle by means of gravity and fall into a pit at the bottom of the microchannel of the chip body;

step twelve, starting the air pump motor, electrifying the first air inlet control valve 422 for controlling the on-off of the air path of the sample adding groove 501 for placing the first reagent, allowing the air to enter the sample adding groove 501 for placing the first reagent, pressing the first reagent into the chip body, and then powering off the first air inlet control valve 422 electrified in the step, so that the first reagent stops flowing;

step thirteen, starting an air pump motor, controlling a first air pumping control valve 423 which is switched on and off for controlling an air path of a waste liquid tank 503 into which the solution with cells is put to be electrified, changing the air pressure in the waste liquid tank 503 so as to drive the liquid in the micro-channel to flow, pumping a first reagent into the micro-channel, flushing redundant cells, only leaving a proper amount of cells in the chip body, and then switching off the first air pumping control valve 423 which is electrified in the step so as to stop the flow of the solution in the micro-channel;

fourteen, electrifying a first air inlet control valve 422 for controlling the on-off of an air path of the sample adding groove 501 for placing the solution with the magnetic beads, enabling the gas to enter the sample adding groove 501 for placing the solution with the magnetic beads, pressing the solution with the magnetic beads into the chip body, and then powering off the first air inlet control valve 422 electrified in the step, so that the solution with the magnetic beads stops flowing;

step fifteen, starting an air pump motor, controlling a first air pumping control valve 423 which is switched on and off by an air path of a waste liquid tank 503 which is filled with the solution with cells to be electrified, changing the air pressure in the waste liquid tank 503 so as to drive the liquid in the micro-channel to flow, pumping the solution with magnetic beads into the micro-channel, and then switching off the first air pumping control valve 423 which is electrified in the step so as to stop the flow of the solution in the micro-channel;

sixthly, starting an air pump motor, electrifying a first air inlet control valve 422 for controlling the on-off of an air path of the sample adding groove 501 for placing the first reagent, enabling air to enter the sample adding groove 501 for placing the first reagent, pressing the first reagent into the chip body, and then powering off the first air inlet control valve 422 electrified in the step, so that the first reagent stops flowing;

seventhly, starting an air pump motor, controlling a first air pumping control valve 423 which is switched on and off for controlling an air path of a waste liquid tank 503 into which the solution with cells is put to be electrified, changing the air pressure in the waste liquid tank 503 so as to drive the liquid in the micro-channel to flow, pumping a first reagent into the micro-channel, flushing redundant magnetic beads, only leaving a proper amount of magnetic beads in the chip body, and then switching off the first air pumping control valve 423 which is electrified in the step so as to stop the flow of the solution in the micro-channel;

eighteen, electrifying the first air inlet control valve 422 for controlling the on-off of the air path of the sample adding groove 501 for placing the second reagent, allowing the air to enter the sample adding groove 501 for placing the second reagent, pressing the second reagent into the chip body, and then powering off the first air inlet control valve 422 powered in the step, so as to stop the flow of the second reagent;

nineteenth, starting an air pump motor, controlling a first air pumping control valve 423 which is switched on and off by an air path of a waste liquid tank 503 into which the solution with cells is put to be electrified, so that the air pressure in the waste liquid tank 503 is changed, thereby driving the liquid in the micro-channel to flow, pumping a second reagent into the micro-channel, carrying out biochemical reaction, combining RNA of the cells with the molecular structure on the surface of the magnetic beads to form RNA with the magnetic beads, and then switching off the first air pumping control valve 423 which is electrified in the step, thereby stopping the flow of the solution in the micro-channel;

twenty, starting the feeding motor 711, driving the magnet arm 73 to extend into the position right above the chip component 52 by the feeding motor 711, then electrifying the first connecting block 721, enabling the first connecting block 721 to adsorb the second connecting block 722 with the magnet arm 73 to move towards the direction close to the chip component 52 along the vertical direction, and enabling the magnet arm 73 to adsorb the RNA with magnetic beads in the microchannel so as to enable the RNA to be suspended in the microchannel;

in the twenty-first step, the first connecting block 721 is powered off, the feeding elastic member 723 resets the second connecting block 722 and the magnet arm 73 along the vertical direction, the feeding motor 711 is started, and the feeding motor 711 resets the magnet arm 73 along the horizontal direction;

twenty-two, the first air inlet control valve 422 for controlling the on-off of the air path of the sample adding groove 501 for placing the third reagent is electrified, the gas enters the sample adding groove 501 for placing the third reagent, the third reagent is pressed into the chip body, and then the first air inlet control valve 422 electrified in the step is powered off, so that the third reagent stops flowing;

twenty-third, starting the air pump motor, controlling the second air pumping control valve 424 which is switched on and off by the air path of the waste liquid tank 503 which is filled with the solution with cells to be electrified, changing the air pressure in the sample collection tank, thereby driving the liquid in the micro-channel to flow, pumping the third reagent into the micro-channel, collecting the RNA with magnetic beads into the sample collection tank, and then switching off the second air pumping control valve 424 which is electrified in the step, thereby stopping the flow of the solution in the micro-channel;

twenty-four steps, starting the box cover motor body 411, driving the box cover ball screw and the box cover conveying assembly 412 by the box cover motor body 411 to drive the box cover assembly 42 to move upwards along the vertical direction, and separating the box cover assembly 42 from the box body assembly 32;

twenty-five, starting the two first box motors 311 simultaneously, wherein the first box motors 311 drive the box ball screws 312 to drive the box assembly 32 to extend outwards along the horizontal direction;

twenty-six, taking out the RNA with the magnetic beads from the sample collection groove, and putting the chip structure 5 into a specified garbage collection box;

twenty-seventh, the two first box motors 311 are started simultaneously, and the first box motors 311 drive the box ball screws 312 to drive the box assembly 32 to reset to a position right below the box cover assembly 42 along the horizontal direction;

twenty-eight steps, start lid motor body 411, lid motor body 411 drive lid ball and lid transport assembly 412 drive lid subassembly 42 along vertical direction downstream, and lid subassembly 42 and box body subassembly 32 laminating, so far, whole experiment is all ended.

It should be noted that, in the above steps, in order to meet the temperature requirements of different reagents and biochemical reactions, the chip assembly 52 and the chamber are heated or cooled by adjusting the heat conducting assembly 323, specifically, the reagent in the chip assembly 52 is heated by the peltier element and the heat conducting element 3231, and the reagent in the chip assembly 52 is cooled by the heat dissipating element 3232 and the heat dissipating fan, and the specific temperature adjusting process is not embodied in the above process. In practice, the appropriate temperature for each step can be realized by programming the controller, and the temperature sensor can detect the temperature of the heat-conducting member 3231 in real time to control the temperature of the reagent. When the single-cell nucleic acid processing apparatus is in operation, the heat dissipation fan is always in an on state to dissipate heat from the chip assembly 52 and the chamber.

The embodiment only shows one operation mode of the single-cell nucleic acid processing instrument, and the specific operation mode of the single-cell nucleic acid processing instrument has different processes due to different application scenes and is specifically set according to actual needs.

It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.

Claims (13)

1. A single-cell nucleic acid processing instrument, comprising:

a frame (11);

the air pump assembly is arranged on the rack (11);

the box body structure comprises a box body driving assembly (31) and a box body assembly (32), wherein the box body driving assembly (31) is arranged on the rack (11), the box body assembly (32) is arranged at the output end of the box body driving assembly (31), the box body driving assembly (31) can drive the box body assembly (32) to move along the horizontal direction, and a placing groove (30) is formed in the box body assembly (32);

the box cover structure comprises a box cover driving component (41) and a box cover component (42), wherein the box cover driving component (41) is arranged on the rack (11), the box cover component (42) is arranged at the output end of the box cover driving component (41) and is positioned above the box body component (32), the box cover driving component (41) can drive the box cover component (42) to move along the vertical direction so that the box cover component (42) is covered on the box body component (32), the box cover component (42) comprises a box cover body (421), an air inlet hole group and an air outlet hole group are arranged on the box cover body (421), and the air inlet hole group and the air outlet hole group are communicated with the air pump component;

chip structure (5), set up in standing groove (30), be equipped with application of sample groove (501), sample groove (502), waste liquid groove (503) and microchannel on chip structure (5), application of sample groove (501) with inlet port group intercommunication, sample groove (502) with waste liquid groove (503) all with exhaust port group intercommunication.

2. The single-cell nucleic acid processing apparatus according to claim 1, wherein the cover assembly (42) comprises a first air inlet control valve (422), the air inlet hole set comprises at least two air inlet holes (42102), one first air inlet control valve (422) is disposed at each air inlet hole (42102), the first air inlet control valve (422) is configured to control the connection or disconnection of the air inlet hole (42102) and the air pump assembly, an air inlet channel (42101) is further disposed on the cover body (421), one end of the air inlet channel (42101) is respectively connected to the at least two air inlet holes (42102), and the other end is connected to the air pump assembly;

the lid assembly (42) includes a first air suction control valve (423) and a second air suction control valve (424), the air exhaust hole group comprises two air exhaust holes which are respectively a first air exhaust hole (421041) and a second air exhaust hole (421042), the first air suction hole (421041) is communicated with the waste liquid tank (503) and is provided with the first air suction control valve (423), the second suction hole (421042) is communicated with the sample groove (502) and is provided with the second suction control valve (424), the first air suction control valve (423) and the second air suction control valve (424) are configured to control communication or disconnection of the air suction hole with the air pump assembly, an air exhaust passage (42103) is further arranged on the box cover body (421), one end of the air exhaust passage (42103) is communicated with the two air exhaust holes, and the other end of the air exhaust passage (42103) is communicated with the air pump assembly.

3. The single-cell nucleic acid processing instrument according to claim 2, further comprising a gas circuit integrated block (61), wherein a first connecting channel (6101), a second connecting channel (6102) and a first detecting channel (6103) are disposed on the gas circuit integrated block (61), one end of the first connecting channel (6101) is communicated with the air pump assembly, the other end of the first connecting channel (6101) is communicated with the same end of the second connecting channel (6102) and the first detecting channel (6103), the other end of the second connecting channel (6102) is communicated with the air inlet channel (42101), and the other end of the first detecting channel (6103) is capable of being communicated with a pressure detector;

the air path integrated block (61) is further provided with a third connecting channel (6104), a fourth connecting channel (6105) and a second detection channel (6106), one end of the third connecting channel (6104) is communicated with the air pumping channel (42103), the other end of the third connecting channel (6104) is communicated with the same end of the fourth connecting channel (6105) and the second detection channel (6106), the other end of the fourth connecting channel (6105) is communicated with the air pump assembly, and the other end of the second detection channel (6106) is communicated with the pressure detector.

4. The single-cell nucleic acid processing instrument according to claim 3, wherein the other end of the first detection channel (6103) is interference fitted with a first plug, and the other end of the second detection channel (6106) is interference fitted with a second plug.

5. The single-cell nucleic acid processing instrument of claim 1, wherein the air pump assembly comprises:

the air pump motor is arranged on the rack (11);

the air pump ball screw comprises an air pump screw and an air pump nut, the air pump screw is arranged at the output end of the air pump motor, and the air pump nut is in threaded connection with the air pump screw;

air pump body (21), including air pump section of thick bamboo (211) and air pump push rod subassembly (212), air pump section of thick bamboo (211) set up in frame (11) and inject the pump chamber of giving vent to anger in it, the pump chamber can respectively with the inlet opening group intercommunication, the one end of air pump push rod subassembly (212) with the sealed sliding connection of air pump section of thick bamboo (211), the other end of air pump push rod subassembly (212) with air pump nut fixed connection.

6. The single-cell nucleic acid processing instrument according to claim 1, wherein the cassette cover driving assembly (41) includes two cassette cover motor assemblies respectively located at left and right sides of the cassette cover body (421), each of the cassette cover motor assemblies including:

the box cover motor body (411) is arranged on the rack (11);

lid ball, including lid lead screw and lid nut, the lid lead screw sets up the output of lid motor body (411), lid nut threaded connection is in on the lid lead screw, the lid nut with lid body (421) fixed connection.

7. The single-cell nucleic acid processing instrument according to claim 6, wherein the rack (11) includes a placing plate (12), the cassette cover motor body (411) is provided on a lower side of the placing plate (12), the cassette cover driving assembly (41) further includes two cassette cover transfer assemblies (412), the cassette cover transfer assemblies (412) are provided on an upper side of the placing plate (12), each of the cassette cover transfer assemblies (412) includes:

the first box cover gear (4121) is fixedly arranged at the output end of the box cover motor body (411);

the second box cover gear (4122) is fixedly connected with the box cover screw rod;

a cover transmission belt (4123) engaged with the first cover gear (4121) and the second cover gear (4122).

8. The single-cell nucleic acid processing instrument according to claim 1, wherein the cartridge driving assembly (31) comprises:

the first box body motor (311) is arranged on the rack (11);

box ball screw (312), including box body lead screw and box body nut, the box body lead screw sets up the output of first box body motor (311), box body nut threaded connection be in on the box body lead screw and with box body subassembly (32) fixed connection, first box body motor (311) can drive the box body lead screw rotates so that the box body nut drives box body subassembly (32) are followed the horizontal direction motion.

9. The single-cell nucleic acid processing instrument according to claim 1, wherein the cartridge driving assembly (31) comprises:

the second box body motor is arranged on the rack (11);

box body gear drive subassembly, including engaged box body gear and box body rack, the box body gear is fixed to be set up the output of second box body motor, the box body rack slides and sets up in frame (11) and with box body subassembly (32) fixed connection, second box body motor can drive box body gear revolve so that the box body rack drives box body subassembly (32) are followed the horizontal direction motion.

10. The single-cell nucleic acid processing apparatus according to claim 1, wherein the cartridge assembly (32) comprises a cartridge body (321), a heat-insulating frame (322), and a heat-conducting assembly (323), the heat conducting component (323) and the box body (321) enclose a cavity with an open upper end, the heat conducting component (323) comprises a heat conducting element (3231), a Peltier and a heat radiating element (3232) which are sequentially stacked, the Peltier is located below the heat-conducting member (3231) and is capable of heating and cooling the chamber, the heat dissipation piece (3232) is positioned below the Peltier, the heat preservation frame (322) is arranged along the circumferential direction of the heat conduction piece (3231) and the heat preservation frame (322) is clamped between the heat conduction piece (3231) and the heat dissipation piece (3232), be equipped with on heat preservation frame (322) and dodge hole (3220), dodge hole (3220) just to heat-conducting element (3231) with heat dissipation piece (3232) sets up.

11. The apparatus for processing single-cell nucleic acid according to claim 10, wherein the heat sink (3232) is a heat sink or a heat sink plate, and a cooling channel is disposed in the heat sink, and the cooling water in the cooling channel can cool the heat sink plate.

12. The single-cell nucleic acid processing apparatus according to claim 1, further comprising a magnet feeding structure including a magnet driving assembly (71), a connecting assembly (72), and a magnet arm (73), wherein the magnet driving assembly (71) is disposed on the frame (11), the connecting assembly (72) is disposed at an output end of the magnet driving assembly (71), the connecting assembly (72) includes a first connecting block (721), a second connecting block (722), and a feeding elastic member (723), wherein the second connecting block (722) is slidably disposed on the first connecting block (721), the feeding elastic member (723) is interposed between the first connecting block (721) and the second connecting block (722), and the first connecting block (721) can attract the second connecting block (722) when energized, and when the outage feed elastic component (723) can reset second connecting block (722), magnet arm (73) are fixed to be set up on second connecting block (722), magnet drive assembly (71) can drive coupling assembling (72) drive magnet arm (73) move along the horizontal direction so that magnet arm (73) are just right chip structure (5), when first connecting block (721) circular telegram magnet arm (73) can be followed second connecting block (722) are close to along the vertical direction orientation the direction motion of chip structure (5).

13. The single-cell nucleic acid processing instrument according to claim 1, wherein the chip structure (5) comprises a chip cartridge (51) and at least one chip component (52), at least one chip component (52) being disposed in the chip cartridge (51), the chip cartridge (51) being snapped in the placement groove (30).

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