CN105316226A - Lifting mechanism and biochip detection device with same - Google Patents
Lifting mechanism and biochip detection device with same Download PDFInfo
- Publication number
- CN105316226A CN105316226A CN201410365262.8A CN201410365262A CN105316226A CN 105316226 A CN105316226 A CN 105316226A CN 201410365262 A CN201410365262 A CN 201410365262A CN 105316226 A CN105316226 A CN 105316226A
- Authority
- CN
- China
- Prior art keywords
- conditioning unit
- temperature conditioning
- biochip
- hoisting appliance
- magnet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a lifting mechanism and a biochip detection device with the same. The biochip detection device comprises a chip carrying unit, a temperature control unit and a base which are arranged sequentially from top to bottom. The chip carrying unit is used for carrying biochips, and the temperature control unit is used for controlling temperatures of the biochips. The lifting mechanism comprises a driving component and a position detection component. The driving component is used for supporting and driving the temperature control unit to ascend and descend, and the position detection component is used for detecting a vertical position of the temperature control unit.
Description
The divisional application of the application for a patent for invention that the application is application number is 201410237647.6, the applying date, to be May 31, denomination of invention in 2014 be " temperature conditioning unit and possess the bio-chip test device of this temperature conditioning unit ".
Technical field
The invention belongs to field of biological detection, particularly, relate to and be a kind ofly applicable to the temperature conditioning unit of bio-chip test device and possess the bio-chip test device of this temperature conditioning unit.
Background technology
Biochip (biochip), also known as protein chip or gene chip, a large amount of probe molecule be fixed on after on upholder and be with fluorescently-labeled DNA or other sample molecules (such as albumen, the factor or small molecules) hybridize, by detecting the hybridization signal intensities of each probe molecule and then obtaining quantity and the sequence information of sample molecule.Biochip is according to interactional principle special between biomolecules, by biochemical analysis process integration in chip surface, thus realizes the high-throughput rapid detection to DNA, RNA, polypeptide, protein and other biological composition.
Along with the development of biochip technology, on the basis of micro-array chip in early days, have developed micro-fluidic chip again.So-called microfluidic chip technology (microfluidics) is that biological, chemistry, medical analysis process the basic operation unit such as sample preparation, reaction, separation, detection is integrated on chip piece, automatically completes analysis whole process.Micro-fluidic chip can comprise the structure of various micro-scale, and such as fluid channel, micro-valve, Micropump etc., can make to flow back and forth between fluid each position on chip, or for reacting with fluid reagent.Because micro-fluidic chip is in the great potential in the field such as biology, chemistry, medical science, develop into the brand-new research field of the subject crossing such as biology, chemistry, medical science, fluid, electronics, material, a machinery.
In addition, polymerase chain reaction (polymerasechainreaction, PCR) technology is a kind of common technology of DNA amplification, is trace/requisite means of trace level biomolecule detection.Round pcr be utilize DNA in vitro about 95 DEG C high temperature time variations Celsius can become strand, time low temperature (being often about 60 DEG C), primer is combined with the principle of strand by base pair complementarity, temperature regulating is to archaeal dna polymerase optimal reactive temperature (about 72 DEG C) again, the direction composition complementary strand of archaeal dna polymerase along phosphoric acid to five-carbon sugar (5 '-3 ').
Namely, PCR is the three warm working cyclees carrying out sex change, annealing and extension within specified temperatures, and often complete a temperature cycle, the quantity of DNA component is doubled, namely with 2
nform increase progressively.Usually, through 20-30 temperature cycle, DNA will be expanded to 1,000,000 times.The key of PCR reaction is repetition and controlled circulation temperature, can realize the amplification amplification of DNA fragmentation thus, be widely used in scientific research and medical science at present.
The PCR microflow controlled biochip combining above-mentioned micro-fluidic chip and round pcr advantage is a large important development direction of field of biological detection in recent years, when using PCR microflow controlled biochip to detect, needs to carry out heating/cooling control to biochip.In the prior art, also have developed the various bio-chip test device being applicable to PCR microflow controlled biochip.
On this type of PCR microflow controlled biochip, be typically provided with multiple entry/exit liquid mouth and the multiple reactive tanks for holding reactant, this biochip is also formed multiple fluid channel that can connect entry/exit liquid mouth and reactive tank.When using bio-chip test device to detect, testing sample (such as PCR reaction mixture) is flowed into by the fluid inlet be located on biochip, and flows into reactive tank after making this testing sample flow through corresponding fluid channel by suitable pneumatic control.Controlled by the heating/cooling circulated to reactive tank, the testing sample in reactive tank can be made to realize amplification cycles, the DNA amount of this testing sample can be made after n circulation with 2
nform amplification.Finally fluoroscopic examination is carried out to the testing sample after amplification.Such as irradiate by laser source and send fluorescence, gathered by photosensor and after opto-electronic conversion the electrical signal of exportable fluorescent value.In above-mentioned biochip, multiple reactive tank can be such as be respectively used to the example reaction groove of testing sample and/or the contrast reactive tank for control group.In use, need to carry out heating/cooling control to multiple reactive tank simultaneously.
At present, PCR is being carried out in the technology of temperature control, existing technology TEC (thermoelectriccooler, TEC) is applied in PCR temperature control.Such as, in above-mentioned bio-chip test device, the temperature conditioning unit using TEC as heating/refrigerating parts can be used to carry out heating/cooling to the multiple reactive tanks on biochip simultaneously.By changing, the sense of current flowing into TEC can make its cold junction, hot junction exchanges, and by regulating size of current then can control its refrigerating capacity and thermal discharge, effectively realizes PCR temperature control thus by TEC.
Particularly, above-mentioned temperature conditioning unit is arranged at below the supporting rack being placed with biochip, when heating up, using TEC as thermal source, by TEC, the heating unit be such as made up of metal block contacted with the lower surface of this supporting rack is heated up, by the heat conduction of supporting rack and then can heat biochip, PCR temperature-rise period can be realized thus.And when lowering the temperature, the sense of current that can change TEC using by this TEC as cooling source, by TEC, above-mentioned heating unit is lowered the temperature, and then biochip is lowered the temperature.Controlled by the heating/cooling circulated to biochip, thus the reactant be contained in each reactive tank of this biochip can be made to realize amplifying amplification.
In addition, when using above-mentioned bio-chip test device to detect, need to wash away to wash away impurity to biochip toward contact.Usual needs add biomagnetic beads in testing sample, and biomagnetic beads can be adsorbed mutually with required test substance in testing sample and combine.Thus, also need to add magnet in above-mentioned heating unit, this magnet can adsorb mutually across the substrate of biochip and the biomagnetic beads in reactive tank, thus test substance and magazins' layout needed for can making to combine with this biomagnetic beads, impurity is discharged from biochip by waste liquid port.And in order to arrange this magnet in the heating element, in the prior art, the means usually adopted be on the heating element slot to place magnet in all partly overlapping position in vertical direction with each reactive tank.
But, due to slotting with the partly overlapping position of each reactive tank on the heating element, thus cause the disappearance of heating unit in this position due to fluting, the heating rate to each reactive tank and homogeneity can be affected thus, thus desirable heats can not be obtained.Especially for the biochip with the multiple reactive tanks comprising the example reaction groove for testing sample and the contrast reactive tank for control group, existence due to above-mentioned magnet groove can affect heating rate and the homogeneity of each reactive tank, thus can not obtain equal heating condition, and then detected result accurately cannot be obtained.
In addition, in existing bio-chip test device, usually manually biochip is positioned in supporting rack, after placing biochip, by components such as cylinders, this supporting rack is from top to bottom pressed on temperature conditioning unit, but the accurate control of the position between biochip and the temperature conditioning unit of below cannot be realized.
Summary of the invention
In view of the above, technical problem to be solved by this invention is the bio-chip test device providing a kind of temperature conditioning unit for bio-chip test device and possess this temperature conditioning unit, can improve the heats for biochip reaction groove.
In order to solve the problems of the technologies described above, according to an aspect of the present invention, provide a kind of temperature conditioning unit for bio-chip test device, described temperature conditioning unit comprises the heating unit be arranged at below biochip, the upper surface of described heating unit is used for contacting with the lower surface of described biochip, and being formed with opening for holding magnet at described heating unit with the be separated by position of predetermined distance of described upper surface, described opening is overlapping at least partly in vertical direction with the reactive tank accommodating biomagnetic beads of described biochip.
According to the present invention, the heating unit contacted with the lower surface of biochip by upper surface is heated biochip.And being formed with opening for holding magnet at heating unit with the be separated by position of predetermined distance of upper surface, this opening is overlapping at least partly in vertical direction with the reactive tank accommodating biomagnetic beads of biochip.Thus, the magnet be inserted in this opening can adsorb mutually across the substrate of biochip and the biomagnetic beads in reactive tank, thus test substance and magazins' layout needed for being conducive to making combining with this biomagnetic beads.Further, owing to not offering any groove on the upper surface of the heating unit contacted with the lower surface of biochip, thus can carry out homogeneous heating to whole conversion zone, heating rate and the homogeneity of each reactive tank can not be affected.Therefore, adopt temperature conditioning unit of the present invention, Detection results accurately can be obtained.
In the present invention, temperature conditioning unit can also comprise the temperature detecting member be located on described heating unit.
According to the present invention, by being located at the temperature detecting member on heating unit, effectively can detect the temperature of heating unit, thus be conducive to carrying out temperature control to biochip.Preferably, this temperature detecting member can be such as the one or more temperature sensors be arranged on above-mentioned heating unit.
In the present invention, the surface of described heating unit can be formed as specular surface.
According to the present invention, mirror process is carried out to the surface of heating unit, heating unit can be enable to contact with biochip supporting rack lower surface fully, reduce heat transfer further uneven.
In the present invention, the surface of described heating unit can be formed as passive surface.
According to the present invention, by carrying out Passivation Treatment to the surface of heating unit, heating unit can be prevented to be oxidized, the work-ing life of heating unit can be extended thus.
In the present invention, described heating unit can be made up of red copper.
According to the present invention, heating unit can by making the various metals of heat conduction, especially red copper.The heating unit be made up of red copper is higher than traditional aluminum heating unit thermo-efficiency, thus can more be conducive to realizing controlling the temperature of biochip.
In the present invention, described heating unit comprises the base portion abutted with heating source and the protuberance be arranged on described base portion, the upper surface of described protuberance contacts with the lower surface of described biochip and described opening is formed on described protuberance, and described protuberance is formed as possessing two parallel-segment and be connected to the I shape shape of the vertical section between described two parallel-segment, described vertical section is positioned at the below of the reactive tank of the need heating of described biochip.
According to the present invention, heating unit comprises the base portion abutted with the heating source of such as TEC element, thus can effectively be heated heating unit by heating source; And this heating unit also comprises the protuberance being formed as I shape be located at above base portion, by the below making the vertical section of this I shape protuberance be positioned at the reactive tank of the need heating of biochip, heating region can be made only to be integrated into biochip needs below the reactive tank that carries out heating, thus conduct heat away can be reduced as far as possible, improve heating/cooling efficiency and speed.
In the present invention, described temperature conditioning unit also can comprise and makes described magnet telescopically be inserted in magnet drives mechanism in described opening.
According to the present invention, effectively magnet can be inserted in the opening of above-mentioned heating unit by magnet drives mechanism, or shift out magnet from this opening.Thus, when needing the biomagnetic beads in absorption reaction groove, magnet can be inserted in above-mentioned opening, and when not needing to carry out this absorption, magnet be shifted out from this opening.
In the present invention, described magnet drives mechanism can comprise: for the magnet holder of accommodating described magnet; With the propulsion members for making the described magnet movement be placed in described magnet holder.
The magnet telescopically be placed in magnet holder can be effectively made to be inserted in above-mentioned opening by propulsion members.
In the present invention, described magnet drives mechanism also can comprise and being connected between described propulsion members and described magnet to make the elastic component that abuts against of surface, the innermost of described magnet and described opening.
According to the present invention, due to when using magnet, needing to make magnet as much as possible close to biomagnetic beads, thus needing magnet to be as far as possible in depth inserted in the opening on above-mentioned heating unit.And by being connected to the elastic component between propulsion members and magnet, can, when using this magnet adsorption biomagnetic beads, this magnet be kept abut against with the innermost surface of opening, this magnet can be remained at the innermost of opening thus.
In the present invention, described propulsion members can comprise: step motor; Be connected with described step motor and driven the screw rod rotated by described step motor; Engage with described screw flight with the nut of movement on described screw rod; Be arranged at the web member be connected in described magnet holder and with described magnet movably, make described web member drive described magnet to move by the movement of described nut; Described elastic component is connected between described nut and described web member.
According to the present invention, by step motor rotary screw, move forward and backward to make the nut engaged with this screw flight, and then drive the magnet be connected with this nut to move, and above-mentioned elastic component is connected between nut and magnet, when nut constantly moves forward, this magnet can be kept abut against with the innermost surface of opening thus.
In addition, another aspect of the present invention, provides a kind of bio-chip test device possessing above-mentioned temperature conditioning unit.
According to bio-chip test device of the present invention, homogeneous heating can be carried out to whole conversion zone, heating rate and the homogeneity of each reactive tank can not be affected.Therefore, Detection results accurately can be obtained.
The present invention can comprise the arbitrary combination of at least two structures disclosed in claims and/or specification sheets and/or accompanying drawing.Especially, the present invention comprises the plural arbitrary combination of every claim of claims.
According to following embodiment also with reference to accompanying drawing, above-mentioned and other object of the present invention, feature and advantage will be understood better.
Accompanying drawing explanation
Fig. 1 shows the structural representation of the bio-chip test device according to an example of the present invention;
Fig. 2 shows the longitudinal sectional view of the A-A line along Fig. 1 of the bio-chip test device shown in Fig. 1;
Fig. 3 schematically shows the vertical view of an example of the heating unit of the temperature conditioning unit for bio-chip test device;
Fig. 4 shows the stereographic map of the heating unit shown in Fig. 3;
Fig. 5 schematically shows the vertical view at biochip place when using magnet drives mechanism urges magnet to adsorb;
Fig. 6 schematically shows the longitudinal sectional view of the B-B line along Fig. 5 of the magnet drives mechanism shown in Fig. 5.
Embodiment
Below in conjunction with accompanying drawing and concrete example, technical scheme of the present invention is described in detail.Fig. 1 and Fig. 2 shows the schematic diagram of the bio-chip test device according to an example of the present invention.Identical or corresponding parts are marked with identical Reference numeral in the drawings and in which, and description is omitted.
As depicted in figs. 1 and 2, the bio-chip test device of this example mainly comprises: from top to bottom set gradually for load biochip chip mounting unit 1, for carrying out temperature controlled temperature conditioning unit 2 and base 3 to biochip.In addition, this bio-chip test device also comprises diagram elliptical for the optical detection unit that detects biochip and the pneumatic control unit etc. for controlling each fluid channel in biochip.Optical detection unit and pneumatic control unit can use the conventional design of this area, and at this, description is omitted.
As shown in Figure 2, said chip mounting unit 1 comprises the supporting rack 12 for loading biochip 11.And above-mentioned temperature conditioning unit 2 comprises the heating unit 20 be arranged at below chip mounting unit 1.Particularly, this heating unit 20 is located at the below of biochip 11.In addition, this temperature conditioning unit 2 also comprises the TEC element 26 be arranged at below heating unit 20, and is located at the radiator element 23 below this TEC element 26.Wherein, TEC element 26 can be used as heating source and the cooling source of this temperature conditioning unit 2, carries out as required heating or lowering the temperature, and controls with the PCR temperature realized in this bio-chip test device.
Fig. 3 and Fig. 4 schematically shows an example of heating unit 20, and Fig. 5 schematically shows the vertical view using magnet drives mechanism 4 to promote biochip 11 place when magnet 41 adsorbs.As shown in Figures 2 to 4, this heating unit 20 can comprise the base portion 22 abutted with the TEC element 26 as heating source.The size of this base portion 22 can be consistent with TEC element 26.Can effectively by TEC element 26, heating unit 20 be heated by arranging this base portion 22.
As shown in Figure 3 and Figure 4, this heating unit 20 also comprises the protuberance 21 be located at above base portion 22.As shown in Figure 2, the upper surface 21a of this protuberance 21 contacts with the lower surface of biochip 11.
And as shown in Figure 3 and Figure 4, this protuberance 21 is formed as to be possessed two parallel-segment 21b, 21c and is connected to the I shape shape of the vertical section 21d between these two parallel-segment 21b, 21c, and this vertical section 21d is positioned at the below (with reference to Fig. 5) of the reactive tank 14-16 of the need heating of biochip 11.As shown in Figure 5, by the below making the vertical section 21d of this I shape protuberance 21 be positioned at the reactive tank 14-16 of the need heating of biochip 11, heating region can be made only to be integrated into biochip 11 needs below the reactive tank 14-16 that carries out heating, thus conduct heat away can be reduced as far as possible, improve heating/cooling efficiency and speed.
Due to, the upper surface 21a of the protuberance 21 of this heating unit 20 is used for contacting with the lower surface of biochip 11, thus can when TEC element 26 produces heat as heating source, this heating unit 20 is heated, and then by protuberance 21 couples of biochip 11(with reference to Fig. 1, Fig. 5) heat.And as shown in Figure 4, be formed with opening 24 for holding magnet at this heating unit 20 with the be separated by position of predetermined distance of its upper surface 21a.Particularly, as shown in Figure 4, this opening 24 is formed on the protuberance 21 of 20.And the afore mentioned rules distance between opening 24 and upper surface 21a can set according to practical situation, such as can according to the material of heating unit, desired by the setting such as the thermo-efficiency that reaches.This opening 24 is overlapping at least partly in vertical direction with the reactive tank (reactive tank 16 shown in Figure 5 and the biomagnetic beads 16a be contained in this reactive tank 16) accommodating biomagnetic beads 16a of biochip 11.
Particularly, as shown in Figure 5, this biochip 11 can have multiple fluid inlets 18 that reactant is flowed into, and for holding multiple reactive tank 14-16 of reactant, and connects multiple fluid channel 17 of fluid inlet 18 and reactive tank 14-16.In the example shown in Fig. 5, show three fluid inlets 18 and corresponding with it three reactive tank 14-16, wherein, reactive tank 14 and 15 is the contrast reactive tanks for control group, and reactive tank 16 is the example reaction grooves for testing sample.But the present invention is not limited thereto, the quantity of fluid inlet and reactive tank can be set according to actual needs.
Further, be usually contained in the testing sample in example reaction groove 16 and can adding biomagnetic beads 16a.Can clearly show that from Fig. 5, the opening 24 of heating unit 20 and the example reaction groove 16 accommodating biomagnetic beads 16a of biochip 11 are overlapping at least partly in vertical direction, and magnet 41 can be made thus to be positioned at the below of this example reaction groove 16.
As shown in Figure 5 and Figure 6, by arranging above-mentioned opening 24, the magnet 41(be inserted in this opening 24 can be made to describe in detail later) across the substrate 11a(be such as made up of glass of biochip 11 with reference to Fig. 5) adsorb with the biomagnetic beads 16a phase in example reaction groove 16, thus test substance and magazins' layout needed for being conducive to making combining with this biomagnetic beads 16a.And, owing to not offering any groove on the upper surface 21a of the heating unit 20 contacted with the lower surface of biochip 11, thus can carry out homogeneous heating to the whole conversion zone of biochip 11, heating rate and the homogeneity of each reactive tank 14-16 can not be affected.Therefore, adopt the temperature conditioning unit 2 possessing heating unit 20 of the present invention, Detection results accurately can be obtained.
Above-mentioned heating unit 20 can by making the various metals of heat conduction, especially red copper.The heating unit be made up of red copper is higher than traditional aluminum heating unit thermo-efficiency, thus can more be conducive to realizing controlling the temperature of biochip.
In addition, also can carry out mirror process to the upper surface 21a on the surface of heating unit 20, especially protuberance 21, heating unit 20 can be made to contact with the lower surface of biochip 11 fully, reduce heat transfer further uneven.Further, also by carrying out Passivation Treatment to the surface of heating unit 20, to prevent heating unit 20 to be oxidized, the work-ing life of heating unit 20 can be extended thus.
In addition, also can set temperature detection means on heating unit 20, effectively can detect the temperature of heating unit 20, thus be conducive to carrying out temperature control to biochip 11.This temperature detecting member can be such as be arranged at the one or more temperature sensors on above-mentioned heating unit 20.Particularly, as shown in Figure 4, temperature detection hole 25 can be offered on this heating unit 20, and in this temperature detection hole 25 mounting temperature sensor.Although Fig. 4 shows two the temperature detection holes 25 be arranged on the protuberance 21 of heating unit 20, the present invention is not limited thereto, can the quantity of set temperature sensor and position according to actual needs.
In the present invention, temperature conditioning unit 2 also can comprise and makes magnet 41 telescopically be inserted in magnet drives mechanism in the opening 24 of above-mentioned heating unit 20.When needing the biomagnetic beads in adsorption sample reactive tank 16, magnet 41 can be inserted in above-mentioned opening 24, and when not needing to carry out this absorption, magnet be shifted out from this opening 24 by this magnet drives mechanism 4.
Fig. 6 schematically shows the longitudinal sectional view of the B-B line along Fig. 5 of the magnet drives mechanism 4 shown in Fig. 5.As shown in Figure 5 and Figure 6, this magnet drives mechanism 4 such as can comprise: for accommodating and support magnet 41 and in the magnet holder 42 of elongated tubular; For making the propulsion members of magnet 41 movement be placed in magnet holder 42.And this magnet drives mechanism 4 also can comprise the elastic component 43 be connected between propulsion members and magnet 41.
Magnet 41 telescopically be placed in magnet holder 42 can be effectively made to be inserted in above-mentioned opening 24 by propulsion members.And when using magnet 41, needing to make magnet 41 as much as possible close to biomagnetic beads 16a, thus needing magnet 41 to be as far as possible in depth inserted in opening 24.And by being connected to the elastic component 43 between propulsion members and magnet 41, can when using this magnet 41 to adsorb biomagnetic beads, this magnet 41 is kept abut against with the innermost surperficial 24a of opening 24 (with reference to shown in Fig. 5 and Fig. 6), this magnet 41 can be remained at the innermost of opening 24 thus.
As shown in Figure 6, above-mentioned propulsion members can specifically comprise step motor 44; Be connected with this step motor 44 and driven the screw rod 45 of rotation by step motor 44; The nut 46 with movement on screw rod 45 is threadedly engaged with screw rod 45; Be arranged at the web member 47 be connected in magnet holder 42 and with magnet 41 movably.Movement by nut 46 makes web member 47 be with moving magnet 41 to move.And above-mentioned elastic component 43 is connected between nut 45 and web member 47.As shown in Figure 5 and Figure 6, this elastic component 43 can be the spring 43 be connected between nut 45 and web member 47.
By step motor 44 rotary screw 45, move forward and backward with the nut 46 making to be threadedly engaged with this screw rod 45, and then be with moving magnet 41 to move by the web member 47 that is connected with magnet 41 and the spring 43 be connected between nut 45 and web member 47.And by arranging above-mentioned elastic component 43, can, when nut 46 constantly moves to the direction of the opening 24 close to above-mentioned heating unit 20, this magnet 41 be kept abut against with the innermost surperficial 24a of opening 24 thus.This magnet 41 can be remained at the innermost of opening 24 thus, to make magnet 41 as much as possible close to biomagnetic beads 16a, can thus effectively can adsorb biomagnetic beads.
By possessing the bio-chip test device of above-mentioned temperature conditioning unit 2, homogeneous heating can be carried out to whole conversion zone, heating rate and the homogeneity of each reactive tank can not be affected.Therefore, Detection results accurately can be obtained.Especially for the biochip with the multiple reactive tanks comprising testing sample reactive tank 16 and control group reactive tank 14,15, each reactive tank can being made to obtain identical heats by bio-chip test device of the present invention, being conducive to the accuracy to detecting.
In addition, get back to Fig. 1 and Fig. 2, bio-chip test device of the present invention also comprises the hoisting appliance for temperature conditioning unit 2.This hoisting appliance can comprise: for supporting and driving the drive member that temperature conditioning unit 2 is elevated; With the position detection component for detecting temperature conditioning unit 2 position in vertical direction.The whole temperature conditioning unit 2 comprising heating unit 20, TEC element 26, radiator element 23 etc. upwards can be promoted by this drive member, the upper surface 21a of the heating unit 20 of this temperature conditioning unit 2 is contacted, to heat biochip 11 with the lower surface of biochip 11.Further, temperature conditioning unit 2 can be made to stop in position by this position detection component.Heating unit 20 pairs of biochips 11 applied pressures can be controlled thus, prevent biochip 11 excessive compression.In addition, the fluid channel 17(of biochip 11 can also be made with reference to shown in Fig. 5) be tightly connected with external valve (illustrate and omit), to prevent gas leakage.
Particularly, as shown in Figure 2, above-mentioned drive member can comprise the step motor 51 be positioned on base 3.This step motor 51 is fixed on base 3 by motor bracket 52.This drive member also comprises and drives by step motor 51 screw member that temperature conditioning unit 2 is elevated.
In the example shown in Fig. 2, screw member can comprise and upwards to extend from step motor 51 and driven the screw mandrel 53 of rotation by step motor 51 and be threadedly engaged with the leading screw 54 moved up and down at this screw mandrel 53 with screw mandrel 53.This leading screw 54 is connected to drive temperature conditioning unit 2 to be elevated with temperature conditioning unit 2.
Particularly, be connected by transom between leading screw 54 with temperature conditioning unit 2.As shown in Figure 2, this transom can comprise the transmission plate 55 be arranged on leading screw 54 and the joint pin 56 be connected between transmission plate 55 and temperature conditioning unit 2.Further, joint pin 56 is also provided with spring 57.This spring 57 extrudes by transmission plate 55 and produces recoverable deformation, and this deflection can provide the power lifting temperature conditioning unit 2, and makes the power that seals between biochip 11 and external valve, and biochip 11 and heating unit 21 can be made to fit tightly.Although be provided with four joint pins 56 and be located at totally four springs 57 on each joint pin 56 respectively in this example, the present invention is not limited thereto, the quantity of spring 57 can be set as required.
In addition, also as shown in Figure 2, in the present invention, above-mentioned leading screw 54 can be formed as trapezoidal screw.By being formed as trapezoidal screw, moving up and down with auto-lock function of temperature conditioning unit 2 can be made, and then temperature conditioning unit 2 can not free landing.Particularly, when step motor 51 is out of service, can provide friction resistance between trapezoidal screw 54 and screw mandrel 53, this friction resistance can stop temperature conditioning unit 2 glide and produce self-locking.Namely the position of temperature conditioning unit 2, is made to control arbitrarily.
In addition, in the present invention, above-mentioned position detection component can comprise the position transducer on the lift path being located at temperature conditioning unit 2.Particularly, in temperature conditioning unit 2 process of rising or falling, the jogging sensor (diagram is omitted) of position transducer can be provided as on its movement path.When this jogging sensor is triggered by temperature conditioning unit 2, produce motion or the stopping of signal control step motor 51.
See figures.1.and.2, hoisting appliance of the present invention also can comprise the guiding mechanism of the movement for guiding temperature conditioning unit 2 in vertical direction.In this example, this guiding mechanism such as can comprise four guideposts 58 being located at temperature conditioning unit 2 four jiaos of places.Only moved up and down by the bootable temperature conditioning unit 2 of this guidepost 58, and do not rotate.
In addition, as shown in Figure 2, chip mounting unit 1 can be provided with top board 13.This top board 13 can push down biochip 11 from top, to make the fluid channel 17(of external valve and biochip 11 that makes provided thereon with reference to Fig. 5) be tightly connected, thus the gas circuit importing biochip 11 is provided.
By the present invention, can after placing biochip 11, the accurate control of the position between the temperature conditioning unit 2 realizing biochip 11 and below.Especially, relative to supporting rack 12 being from top to bottom pressed on by components such as cylinders technology temperature conditioning unit 2 manually can only being placed biochip in prior art, the present invention can realize the autofitting of biochip 11.Such as, can, after biochip 11 is placed in chip tray, this chip tray be moved on supporting rack 12 automatically.Thus, the automatization realizing bio-chip test device can be conducive to.
Under the aim not departing from essential characteristic of the present invention, the present invention can be presented as various ways, therefore the example in the present invention is illustrative rather than definitive thereof, be defined by the claims due to scope of the present invention but not limited by specification sheets, and drop on the scope that claim defines, or all changes in the full scope of equivalents of its scope defined all are understood to include in detail in the claims.
Claims (10)
1. the hoisting appliance for the temperature conditioning unit of bio-chip test device, described bio-chip test device comprise from top to bottom set gradually for load biochip chip mounting unit, for carrying out temperature controlled temperature conditioning unit and base to biochip, it is characterized in that, described hoisting appliance comprises:
For supporting and driving the drive member that described temperature conditioning unit is elevated; With
For detecting the position detection component of described temperature conditioning unit position in vertical direction.
2. hoisting appliance according to claim 1, is characterized in that, described drive member comprises:
Be positioned at the step motor on described base;
Driven by described step motor with the screw member making described temperature conditioning unit be elevated.
3. hoisting appliance according to claim 2, is characterized in that, described screw member comprises:
Upwards extend from described step motor and driven the screw mandrel rotated by described step motor;
Engage with described wire rod thread with the leading screw moved up and down on described screw mandrel, described leading screw is connected with described temperature conditioning unit to drive described temperature conditioning unit to be elevated.
4. hoisting appliance according to claim 3, it is characterized in that, be connected by transom between described leading screw with described temperature conditioning unit, described transom comprises the transmission plate be arranged on described leading screw and the joint pin be connected between described transmission plate and described temperature conditioning unit.
5. hoisting appliance according to claim 4, is characterized in that, also comprises the spring be located on described joint pin.
6. hoisting appliance according to claim 3, is characterized in that, described leading screw is formed as trapezoidal screw.
7. hoisting appliance according to any one of claim 1 to 6, is characterized in that, also comprises the guiding mechanism of the movement for guiding described temperature conditioning unit in vertical direction.
8. hoisting appliance according to claim 7, is characterized in that, described guiding mechanism comprises the guidepost being located at described temperature conditioning unit corner place.
9. the hoisting appliance for the temperature conditioning unit of bio-chip test device, described bio-chip test device comprise from top to bottom set gradually for load biochip chip mounting unit, for carrying out temperature controlled temperature conditioning unit and base to biochip, it is characterized in that, described hoisting appliance comprises drive member, and described drive member comprises:
Be positioned at the step motor on described base; With
Driven by described step motor with the screw member making described temperature conditioning unit be elevated.
10. one kind possesses the bio-chip test device of hoisting appliance according to any one of claim 1 to 9.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410237647.6A CN105302192A (en) | 2014-05-31 | 2014-05-31 | Temperature control unit and biochip detection apparatus having the temperature control unit |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410237647.6A Division CN105302192A (en) | 2014-05-31 | 2014-05-31 | Temperature control unit and biochip detection apparatus having the temperature control unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105316226A true CN105316226A (en) | 2016-02-10 |
Family
ID=55199568
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410237647.6A Pending CN105302192A (en) | 2014-05-31 | 2014-05-31 | Temperature control unit and biochip detection apparatus having the temperature control unit |
| CN201410365262.8A Pending CN105316226A (en) | 2014-05-31 | 2014-05-31 | Lifting mechanism and biochip detection device with same |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410237647.6A Pending CN105302192A (en) | 2014-05-31 | 2014-05-31 | Temperature control unit and biochip detection apparatus having the temperature control unit |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN105302192A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109622086A (en) * | 2019-01-31 | 2019-04-16 | 河南科技大学 | Micro-fluidic chip, manufacturing method and the micro-fluidic detection equipment of preset magnetic bead |
| CN110724626A (en) * | 2019-11-13 | 2020-01-24 | 北京航宇阳光机电技术研究所 | Integrated detection device of biochip |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109187347B (en) * | 2018-10-16 | 2021-09-14 | 无锡科智达科技有限公司 | Detection device for detecting micro-fluidic chip |
| CN111925931A (en) * | 2020-08-25 | 2020-11-13 | 墨卓生物科技(上海)有限公司 | Heating structure of PCR instrument and chip positioning heating method |
| CN116928988A (en) * | 2022-04-02 | 2023-10-24 | 青岛海尔电冰箱有限公司 | Refrigerator and control method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1368555A (en) * | 2001-02-09 | 2002-09-11 | 中国科学院电子学研究所 | DNA-PCR biochip and miniature heat circulator using it |
| CN1995319A (en) * | 2007-01-12 | 2007-07-11 | 北京工业大学 | Multiple passage intelligent temperature-control device facing PCR microfluidic chip |
| CN201060424Y (en) * | 2007-07-27 | 2008-05-14 | 北京工业大学 | Biochip temperature control subpanel |
| CN102985527A (en) * | 2010-04-23 | 2013-03-20 | 纳米生物系统株式会社 | PCR device including two heating blocks |
| CN103725602A (en) * | 2012-10-16 | 2014-04-16 | 常州福生生物技术有限公司 | Thermal cover plate used in nucleic acid amplification detector |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100514244C (en) * | 2005-10-14 | 2009-07-15 | 财团法人工业技术研究院 | Miniature fluid temperature controlling device and method thereof |
| US20070099288A1 (en) * | 2005-11-02 | 2007-05-03 | Affymetrix, Inc. | Microfluidic Methods, Devices, and Systems for Fluid Handling |
| CN101458251B (en) * | 2009-01-04 | 2013-02-27 | 东南大学 | Magnetic particle micro array apparatus for high throughput detecting biomacromolecule and its use method |
| US9335339B2 (en) * | 2009-06-04 | 2016-05-10 | Universal Bio Research Co., Ltd. | Specimen testing device and method thereof |
| RU2559541C2 (en) * | 2009-06-05 | 2015-08-10 | Интедженкс Инк. | Universal system for sample preparing and application in integrated system of analysis |
-
2014
- 2014-05-31 CN CN201410237647.6A patent/CN105302192A/en active Pending
- 2014-05-31 CN CN201410365262.8A patent/CN105316226A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1368555A (en) * | 2001-02-09 | 2002-09-11 | 中国科学院电子学研究所 | DNA-PCR biochip and miniature heat circulator using it |
| CN1995319A (en) * | 2007-01-12 | 2007-07-11 | 北京工业大学 | Multiple passage intelligent temperature-control device facing PCR microfluidic chip |
| CN201060424Y (en) * | 2007-07-27 | 2008-05-14 | 北京工业大学 | Biochip temperature control subpanel |
| CN102985527A (en) * | 2010-04-23 | 2013-03-20 | 纳米生物系统株式会社 | PCR device including two heating blocks |
| CN103725602A (en) * | 2012-10-16 | 2014-04-16 | 常州福生生物技术有限公司 | Thermal cover plate used in nucleic acid amplification detector |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109622086A (en) * | 2019-01-31 | 2019-04-16 | 河南科技大学 | Micro-fluidic chip, manufacturing method and the micro-fluidic detection equipment of preset magnetic bead |
| CN110724626A (en) * | 2019-11-13 | 2020-01-24 | 北京航宇阳光机电技术研究所 | Integrated detection device of biochip |
| CN110724626B (en) * | 2019-11-13 | 2022-05-10 | 北京航宇阳光机电技术研究所 | Integrated detection device of biochip |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105302192A (en) | 2016-02-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8926811B2 (en) | Digital microfluidics based apparatus for heat-exchanging chemical processes | |
| Amasia et al. | Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving | |
| Zhang et al. | Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends | |
| CN105316226A (en) | Lifting mechanism and biochip detection device with same | |
| US11235324B2 (en) | Temperature-cycling microfluidic devices | |
| US7579172B2 (en) | Method and apparatus for amplifying nucleic acids | |
| US9962692B2 (en) | Methods, devices, and systems for fluid mixing and chip interface | |
| Hsieh et al. | Enhancement of thermal uniformity for a microthermal cycler and its application for polymerase chain reaction | |
| CN101107507B (en) | Temperature controller for small fluid samples having different heat capacities | |
| JP2015532094A (en) | Honeycomb tube | |
| WO2006124458A2 (en) | Method and device for conducting biochemical or chemical reactions at multiple temperatures | |
| CN102199529A (en) | Biochip hybridization system | |
| CN107051598B (en) | PCR microfluidic chip, preparation and use methods thereof and PCR equipment | |
| US20160251698A1 (en) | Analysis Unit for Carrying Out a Polymerase Chain Reaction, Analysis Device, Method for Operating such an Analysis Unit, and Method for Producing such an Analysis Unit | |
| CN101145060A (en) | Temperature-controlled array for micro-fluidic chip | |
| Kulkarni et al. | A review on recent advancements in chamber-based microfluidic PCR devices | |
| CN102154261B (en) | Device for performing nucleic acid amplification in micro-fluidic chip | |
| Chien et al. | A micro circulating PCR chip using a suction-type membrane for fluidic transport | |
| US20210008550A1 (en) | Microfluidic reaction vessel array with patterned films | |
| CN205329004U (en) | Dull and stereotyped isothermal nucleic acid increase chip | |
| JP7132158B2 (en) | Temperature controller and nucleic acid amplifier | |
| US11701661B2 (en) | Method and system for localized heating by illumination of patterned thin films | |
| CN110205242B (en) | Microfluidic chip assembly for rapidly realizing digital PCR (polymerase chain reaction) and application thereof | |
| US20240139746A1 (en) | Method and system for localized heating by illumination of patterned thin films | |
| Sheu et al. | A 3D-printed oscillatory polymerase chain reaction system using a single heater |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160210 |