CN114146738B

CN114146738B - PCR kit and hot cover taking and unloading device

Info

Publication number: CN114146738B
Application number: CN202111457396.9A
Authority: CN
Inventors: 俞郴荣; 徐涛; 骆志成; 樊伟东
Original assignee: HANGZHOU ALLSHENG INSTRUMENTS CO Ltd
Current assignee: HANGZHOU ALLSHENG INSTRUMENTS CO Ltd
Priority date: 2021-12-02
Filing date: 2021-12-02
Publication date: 2023-05-26
Anticipated expiration: 2041-12-02
Also published as: CN114146738A

Abstract

The utility model relates to a PCR kit and hot lid are got and are unloaded device, the PCR kit of this application can be used for with the hot lid cooperation of PCR appearance and use, at least one PCR reaction hole has been seted up to the top surface of PCR kit, and the top surface of PCR kit just is located PCR reaction hole department and is equipped with first sealing portion, and first sealing portion is configured to be used for forming sealed cooperation with the hot lid of PCR appearance. So the connection that can disassemble is realized to the hot lid of PCR appearance and heating post accessible elasticity buckle and fixed slot in this application to can realize the dismantlement of the hot lid of PCR appearance on the heating post voluntarily through first actuating mechanism, improve degree of automation, improve work efficiency.

Description

PCR kit and hot cover taking and unloading device

Technical Field

The application relates to the field of gene detection, in particular to a PCR (polymerase chain reaction) kit and a hot cover taking-off device.

Background

In the scientific and medical fields, 1 or more thermal reaction processes are often accompanied during the pretreatment of gene sequencing. For example: in PCR amplification reactions in detection techniques based on the polymerase chain amplification (PCR) principle, during the reaction, a thermal reaction with a high temperature, a long reaction time or a rapid temperature change is accompanied, and due to the rapid temperature change during PCR gene amplification and the large temperature difference between the PCR gene amplification and the ambient temperature, a serious evaporation phenomenon of the reagent is unavoidable, so that aerosol is generated. If aerosol overflows, pollution problems can be generated, normal reaction is affected, accuracy of biochemical detection results is affected, and even if paraffin oil is used for sealing, the problem of aerosol cannot be completely eradicated due to the fact that the paraffin oil is easy to volatilize. Therefore, a thermal cover with higher temperature is often arranged above the PCR test tube at present, so that not only can the overflow of aerosol be avoided, but also the reagent can be prevented from condensing on the thermal cover above due to the fact that the thermal cover is heated, and the accuracy of a biochemical detection result is ensured.

However, the thermal cover of the PCR instrument in the prior art is manually installed, for example, a cover is connected with a PCR test tube manually through screwing, an automatic spring pressing device or an aluminum block pressing mode, and then a heating device is added above the cover manually, so that the operation is troublesome, the working efficiency is low, and the degree of automation is low. In addition, in the prior art, the sealing performance of the thermal cover of the PCR instrument is poor, and because the thermal cover of the PCR instrument in the prior art is often one cover corresponding to a plurality of PCR test tubes, each thermal cover of the PCR instrument can be sealed when a plurality of test tubes are used simultaneously.

Disclosure of Invention

The purpose of this application is to provide a PCR kit and hot lid and get and unload device, and its degree of automation is high.

Embodiments of the present application are implemented as follows:

in a first aspect, the present application provides a PCR instrument thermal cover comprising: at least one heat conduction cylinder is provided with at least one connecting hole on the inner side wall of each heat conduction cylinder, and at least one elastic buckle is connected on the inner side wall of each connecting hole.

In one embodiment, at least one sealing post is protruded on the outer bottom surface of the heat conducting cylinder.

In one embodiment, the sealing post is provided with a fixing collar on a side wall of one end far away from the heat conducting barrel.

In an embodiment, a sealing ring is sleeved on the sealing column, and the sealing ring is arranged between the fixing convex ring and the heat conducting cylinder.

In one embodiment, the sealing post is provided with a central hole.

In one embodiment, the heat conduction cylinder comprises a chassis and a frame, wherein the bottom end of the frame is connected to the outer edge of the chassis, the top end of the frame is provided with an opening, and a flanging is outwards convexly arranged; the turnup is provided with a notch.

In an embodiment, at least one limiting raised line is arranged on the side wall of the heat conducting cylinder, and the limiting raised line extends to the flanging.

In a second aspect, the present application provides a PCR kit, which can be used in cooperation with the PCR instrument thermal cover of any one of the embodiments, wherein the top surface of the PCR kit is provided with at least one PCR reaction hole, the top surface of the PCR kit and the PCR reaction hole are provided with a first sealing portion, and the first sealing portion is configured to form sealing cooperation with the PCR instrument thermal cover.

In one embodiment, the first sealing portion includes a positioning collar for face sealing with an outer bottom surface of the heat conductive cylinder.

In one embodiment, the top surface of the PCR kit is provided with at least one thermal cover storage hole.

In an embodiment, the thermal cover storage hole is a step hole, the thermal cover storage hole comprises a first hole and a second hole which are sequentially connected from top to bottom, the aperture of the first hole is larger than that of the second hole, and a limiting bump is arranged on the inner wall of the first hole in a protruding mode.

In a third aspect, the present application provides a thermal cover removing device, which can be used in cooperation with the thermal cover of the PCR instrument according to any one of the above embodiments, and the thermal cover removing device includes: the device comprises a base plate, a taking-off moving plate, a first driving mechanism, at least one cover-off top piece and at least one heating column, wherein the taking-off moving plate is provided with at least one open slot; the cover-unloading top piece is connected to the removing and unloading motion plate and penetrates through the opening groove; the heating column is connected to the substrate and penetrates through the opening groove, and a fixing clamping groove matched with the elastic buckle is formed in the outer side wall of the heating column; the first driving mechanism is arranged on the base plate and connected with the taking-off and unloading moving plate and used for driving the taking-off and unloading moving plate to move along the axis of the heating column.

In one embodiment, the thermal cover removing device further comprises: and the switching column is arranged between the heating column and the substrate.

In one embodiment, the thermal cover removing device further comprises: the heat insulating backing plate 360 is arranged between the transfer post and the base plate.

In one embodiment, the thermal cover removing device further comprises: and the second driving mechanism 38 is in transmission connection with the substrate and is used for driving the substrate to move.

In one embodiment, the first driving mechanism includes: the electric cylinder is provided with a first perforation on the base plate, the electric cylinder is arranged on the base plate, and a main shaft of the electric cylinder penetrates through the first perforation to be connected with the detachable moving plate.

In one embodiment, the base plate is provided with at least one second through hole, and the detachable moving plate is connected with at least one guide rod, and one guide rod penetrates through one second through hole.

The beneficial effect for prior art is for this application:

the utility model provides a PCR appearance heat lid includes the heat-conducting tube, has set up the elasticity buckle on the heat-conducting tube, is equipped with fixed slot on the heating post of this application, when the hot lid of heating PCR appearance, only need with the hot lid of PCR appearance can overlap on locating the heating post to realize fixedly through elasticity buckle and fixed slot can.

Wherein, this application's hot lid is got and is unloaded device still includes to get and is unloaded the motion board, unloads lid top sheet and first actuating mechanism, need only drive through first actuating mechanism and get and unload the motion board and move down along the axis of 5 heating posts, unload between lid top sheet will insert heating post and the hot lid of PCR appearance to give elasticity buckle a power, make elasticity buckle break away from fixed draw-in groove, thereby realize the dismantlement of the hot lid of PCR appearance on the heating post, later can seal the hot lid of PCR appearance in reaction such as PCR test tube, 8 orifice plates, 96 orifice plates or kit again.

To sum up, the connection that can disassemble can be realized through elasticity buckle and fixed draw-in groove to the hot lid of PCR appearance and heating post in this application to can realize the dismantlement of the hot lid of PCR appearance on the heating post automatically through first actuating mechanism, improve degree of automation, improved work efficiency.

In addition, the sealing column is additionally arranged on the thermal cover of the PCR instrument, and can form sealing fit with the reaction holes of the PCR test tube, the 8-hole plate, the 96-hole plate or the kit and the like, and the sealing effect of the thermal cover of the PCR kit and the PCR instrument is improved through the form of hole sealing.

5 this application is through add first sealing portion on the PCR kit, and first sealing portion can form sealed cooperation with the hot lid of PCR appearance, through the form of face seal, has improved the sealed effect of PCR kit and the hot lid of PCR appearance.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings to be used in the embodiments of the present application will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application, and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person of ordinary skill in the art.

FIG. 1 is a schematic diagram showing a thermal cover of a PCR apparatus according to an embodiment of the present disclosure.

FIG. 2 is a left side view of a thermal cover of a PCR instrument according to one embodiment of the present application.

Fig. 3 is a cross-sectional view in the A-A direction of fig. 2, as shown in an embodiment of the present application.

Fig. 4 is a bottom view of a PCR instrument thermal cover according to an embodiment of the present application.

FIG. 5 is a left side view of a thermal cover of a PCR instrument according to one embodiment of the present application.

FIG. 6 is a schematic diagram showing the installation of a thermal cover of a PCR apparatus and a PCR kit according to an embodiment of the present application.

Fig. 7 is a cross-sectional view in the B-B direction of fig. 6, as shown in an embodiment of the present application.

Fig. 8 is a schematic structural view of a thermal cover removing device according to an embodiment of the present application.

Fig. 9 is a front view of a thermal cover removal device according to an embodiment of the present application.

Fig. 10 is a cross-sectional view in the C-C direction of fig. 9, as shown in an embodiment of the present application.

Fig. 11 is a schematic structural view of a thermal cover removing device according to an embodiment of the present application.

Icon: 100-PCR instrument heat cover; 110-a heat conduction cylinder; 111-chassis; 112-frame; 113-flanging; 114-incision; 115-connecting holes; 116-elastic snap; 117-spacing ribs; 118-receiving holes; 120-sealing the column; 121-a central hole; 122-fixing the convex ring; 130-a sealing ring; 140-connecting rods; 200-PCR kit; the top surface of the 201-PCR kit; 210-PCR reaction well; 211-chamfer faces; 220-a first seal; 230-thermal cover storage aperture; 231-first hole; 232-a second hole; 233-limit bump; 300-a hot cover removing device; 310-substrate; 311-first perforation; 312-second perforations; 320-removing the motion plate; 321-open slots; 330-a cover release topsheet; 340-heating the column; 341-fixing the clamping groove; 350-switching columns; 360-heat insulation backing plate; 370-a first drive mechanism; 371-electric cylinder; 372-guide bar; 380-a second drive mechanism.

Detailed Description

The terms "first," "second," "third," and the like are used merely for distinguishing between descriptions and not for indicating a sequence number, nor are they to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal," "vertical," "overhang," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present application, it should be noted that, directions or positional relationships indicated by terms such as "inner", "outer", "left", "right", "upper", "lower", etc. are directions or positional relationships based on those shown in the drawings, or those that are conventionally put in use for the product of the application, are merely for convenience of description and simplification of the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be configured and operated in a specific direction, and therefore should not be construed as limiting the present application.

In the description of the present application, unless explicitly stated and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements.

Gene detection is a medical detection technology, and by extracting nucleic acids in peripheral venous blood, tissues and other body fluids of a detected person, DNA molecules or RNA molecule information in cells of the detected person is analyzed through detection equipment, so that the gene information of the detected person is known, and the etiology or the disease risk is determined. It should be understood that gene testing is not limited to testing humans, but includes testing animals and plants.

The nucleic acid extraction is a pretreatment process of gene detection, specific primer and probe design is carried out according to the sequence of known nucleic acid, the designed primer is synthesized, the extracted nucleic acid is used as a template for fluorescent quantitative PCR experiment, and the yin-yang of a target sample is judged according to fluorescent signals. Nucleic acid extraction is a key step in gene detection, and the quality of the obtained nucleic acid directly influences the success or failure of downstream experiments.

The technical solutions of the present application will be clearly and completely described below with reference to the accompanying drawings.

Referring to fig. 1, a schematic structure diagram of a thermal cover 100 of a PCR instrument according to an embodiment of the disclosure is shown. The thermal cover 100 of the PCR instrument can be applied to biochemical detection equipment such as a PCR instrument. The PCR instrument heat cover 100 includes: the heat conduction tube 110, the heat conduction tube 110 has a receiving hole 118, and the shape of the receiving hole 118 may be various shapes such as a cylinder, a truncated cone, a triangular prism, a rectangular parallelepiped, a pentagonal prism, a hexagonal prism, or a heptagon prism. At least one connection hole 115 is provided on the inner sidewall of each heat conductive cylinder 110, and at least one elastic buckle 116 tilting inwards is connected to the inner sidewall of each connection hole 115.

It should be noted that one elastic buckle 116 may be disposed in one connecting hole 115, or a plurality of elastic buckles 116 may be disposed in one connecting hole 115. One connection hole 115 may be provided on one heat conductive cylinder 110, or a plurality of connection holes 115 uniformly distributed may be provided on one heat conductive cylinder 110. The thermal cover 100 of the PCR instrument may have a plurality of thermal conductive cylinders 110, or the thermal cover 100 of the PCR instrument may be provided with only one thermal conductive cylinder 110. The connection hole 115 may be a through hole penetrating through a sidewall of the heat conductive cylinder 110 or a blind hole. As shown in fig. 1, the thermal cover 100 of the PCR instrument is provided with only one heat conduction tube 110, a connection hole 115 in the form of a through hole is provided in the heat conduction tube 110, and an elastic buckle 116 is provided in one connection hole 115.

The thermal cover 100 of the PCR instrument in this embodiment is fixedly matched with any columnar member through the heat conduction tube 110 and the elastic buckle 116, and if the columnar member is moved by the three-dimensional motion system, the thermal cover 100 of the PCR instrument can be moved, so that full automation of biochemical detection equipment such as the PCR instrument can be realized.

The heat conducting tube 110 may be made of a material with good thermal conductivity, such as medical plastic, so that the PCR instrument heat cover 100 may be sleeved on a columnar heater (heating column 340 shown in the following figure) and uniformly heat the PCR instrument heat cover 100, and meanwhile, the PCR instrument heat cover 100 may be moved through the heating column 340, so as to facilitate full automation of biochemical detection equipment, such as PCR instrument.

At least one sealing post 120 is protruded on the outer bottom surface of the heat conductive cylinder 110. In this embodiment, by adding the sealing column 120 on the PCR apparatus thermal cover 100, the sealing column 120 can form sealing fit with a reaction hole (PCR reaction hole 210 shown in the following figure) at the PCR test tube, 8-hole plate, 96-hole plate or kit, and the sealing effect of the PCR kit 200 and the PCR apparatus thermal cover 100 is improved by the form of hole sealing.

Wherein the outer diameter of the heat conductive cylinder 110 is larger than the outer diameter of the sealing post 120. The heat conduction tube 110 and the sealing column 120 may be integrally formed, welded, screwed, inserted, or the like. In this embodiment, the heat conductive tube 110, the sealing post 120 and the elastic buckle 116 are all made of medical plastic, so that the problems of poor water absorption and heat conductivity caused by soft rubber materials can be avoided.

It should be noted that, one sealing post 120 on one heat conducting tube 110 may be provided, and then one heat conducting tube 110 may be matched with one PCR reaction hole 210. The sealing columns 120 on one heat conduction cylinder 110 may be provided with a plurality of sealing columns, so that one heat conduction cylinder 110 can be matched with a plurality of PCR reaction holes 210, and the problem of differential porous sealing can be avoided due to the arrangement of the plurality of sealing columns 120.

In this embodiment, the sealing column 120 on one heat conduction tube 110 is provided with one, so that the sealing device can be applied to single sample occasions of single hole sealing or single test tube sealing, and has good sealing effect. When the thermal cover 100 of a plurality of PCR instruments is adopted, the thermal cover can also be applied to the occasion of porous sealing, and the problem of cross contamination caused by the whole surface sealing of porous sealing of 8-hole plates, 96-hole plates and the like is avoided.

Referring to FIG. 2, a left side view of a thermal cover 100 of a PCR instrument according to an embodiment of the present application is shown. Referring to fig. 3, a cross-sectional view of fig. 2 in A-A direction is shown according to an embodiment of the present application.

The sealing post 120 may be a solid post or a hollow tube, and in this embodiment, the sealing post 120 is a hollow tube with a central hole 121, and the central hole 121 is not in communication with the receiving hole 118 of the heat conductive cylinder 110. The arrangement of the central hole 121 can reduce the contact area between the sealing column 120 and the PCR reaction hole 210, and reduce the influence of the thermal cover 100 of the PCR instrument on the PCR reaction.

The sealing post 120 is sleeved with a sealing ring 130, wherein the sealing ring 130 can be made of soft rubber materials such as rubber and silica gel, so as to enhance the sealing effect between the PCR instrument heat cover 100 and the PCR reaction hole 210.

The sealing post 120 is provided with a fixing collar 122 on a side wall of one end remote from the heat conductive cylinder 110. The sealing ring 130 is disposed between the fixing collar 122 and the heat conductive cylinder 110. Wherein, the outer diameter of the sealing post 120 is slightly smaller than the inner diameter of the PCR reaction hole 210, and the outer diameter of the fixing convex ring 122 is adapted to the inner diameter of the PCR reaction hole 210. When the sealing post 120 is inserted into the PCR reaction hole 210, the fixing collar 122 can abut against the inner wall of the PCR reaction hole 210. The fixing convex ring 122 not only can prevent the sealing ring 130 from falling, but also can reduce the contact area between the sealing post 120 and the PCR reaction hole 210 and reduce the influence of the PCR instrument thermal cover 100 on the PCR reaction.

The heat conduction tube 110 comprises a chassis 111 and a frame 112, wherein the bottom end of the frame 112 is connected to the outer edge of the chassis 111, and the frame 112 surrounds the chassis 111 to form a containing hole 118. The top end of the frame 112 is provided with an opening, and a flange 113 is outwards protruded. The flange 113 is provided to facilitate the assembly, disassembly and transfer of the PCR instrument heat cover 100. In the process of disassembling the thermal cover 100 of the PCR instrument, the flange 113 can provide an impetus, so that the moving plate 320 is convenient to disassemble, and the disassembly efficiency is improved. In addition, the flange 113 may provide a support point when the PCR instrument thermal cover 100 is placed. The flange 113 is annular, and has an inner diameter equal to the maximum outer diameter of the frame 112, and an outer diameter thereof can be adjusted accordingly according to the aperture of a thermal cover storage hole 230 (shown in the following figure) of the selected kit.

The flange 113 is provided with a slit 114. At least one limit raised line 117 is arranged on the side wall of the heat conduction tube 110, and the limit raised line 117 extends to the flange 113. The notch 114 and the spacing raised strip 117 can facilitate the storage of the thermal cover 100 of the PCR instrument.

Referring to FIG. 4, a bottom view of a thermal cover 100 of a PCR apparatus according to an embodiment of the present disclosure is shown. In this embodiment, a plurality of heat conductive cylinders 110 are provided, the plurality of heat conductive cylinders 110 are distributed in an array, and two adjacent heat conductive cylinders 110 are connected by a connecting rod 140, a connecting hole 115 and a sealing post 120 are provided on one heat conductive cylinder 110, and an elastic buckle 116 is provided in one connecting hole 115. This embodiment may correspond to a plurality of thermal covers as shown in fig. 1 connected by a connecting rod 140.

Therefore, the embodiment can be applied to single sample or multiple sample occasions of porous sealing or multi-test tube sealing, has good sealing effect, seals single hole or single test tube through one sealing column 120, can avoid the problem of differential porous sealing, and can also properly avoid the problem of complete porous sealing of 8-hole plates, 96-hole plates and the like

Face seals present cross contamination problems. In actual use, each of the heat conductive cylinders 110 may be heated by 5 heating columns 340.

In this embodiment, the flange 113 may not be provided with a notch, and the heat conductive tube 110 may not be provided with the limit protruding strip 117.

In addition, when the thermal cover 100 of the present PCR instrument is matched with the 8-hole plate, 8 heat conductive cylinders 110 may be provided, and the 8 heat conductive cylinders are arranged in a row. When the thermal cover 100 of the PCR instrument is matched with a 96-well plate, 96 heat conducting cylinders 110 can be arranged, and 12 rows of 8 heat conducting cylinders are arranged in one row.

Referring to FIG. 5, a left side view of a thermal cover 100 of a PCR instrument according to an embodiment of the present application is shown. In this embodiment, one heat conductive tube 110 is provided, a plurality of sealing columns 120 distributed in an array are provided on one large heat conductive tube 110, a plurality of connecting holes 115 are provided on one heat conductive tube 110, and an elastic buckle 116 is provided in one connecting hole 115.

5 the present embodiment can be applied to single sample or multiple sample applications of multi-tube or multi-tube seals,

the sealing effect is good, and the sealing column 120 seals a single hole or a single test tube, so that the problem of differentiation of the porous sealing can be avoided, and the problem of cross contamination of the porous sealing of the 8-hole plate, the 96-hole plate and the like due to whole surface sealing can be properly avoided. In actual use, the heat conductive cylinder 110 may be heated by y one large heating column 340.

0, in this embodiment, the heat conduction tube 110 may be a large cylinder, and the flange 113 may be provided with

There may be one or more cutouts, and the plurality of limit ribs 117 on the heat conductive cylinder 110 may be provided.

In addition, when the thermal cover 100 of the present PCR instrument is matched with the 8-well plate, 8 sealing columns 120 may be provided, and the 8 sealing columns 120 are arranged in a row. When the thermal cover 100 of the PCR instrument is matched with a 96-well plate, 96 sealing columns 120 can be arranged, and 8 sealing columns 120 are arranged in a row, and 12 rows are arranged in total.

Referring to FIG. 6, a schematic diagram of the installation of the thermal cover 100 and the PCR kit 200 of the PCR apparatus according to an embodiment of the present disclosure is shown. Referring to fig. 7, a cross-sectional view of the direction B-B of fig. 6 is shown according to an embodiment of the present application. The PCR kit 200 can be used in conjunction with the PCR instrument thermal cover 100 of any of the embodiments of FIGS. 1-5 described above. The top surface 201 of the PCR kit is provided with 1 or more PCR reaction holes 210, the top surface 201 of the PCR kit is provided with a first sealing part 220 at the position of the PCR reaction holes 210, and the first sealing part 220 is configured to form sealing fit with the thermal cover 100 of the PCR instrument. According to the PCR kit, the first sealing part 220 is additionally arranged on the PCR kit 200, the first sealing part 220 can form sealing fit with the PCR instrument heat cover 100, and the sealing effect of the PCR kit 200 and the PCR instrument heat cover 100 is improved through the surface sealing mode.

The first sealing part 220 includes a positioning convex ring, which may be a circular protrusion, for surface sealing with the outer bottom surface of the heat conductive cylinder 110. When 1 PCR reaction hole 210 is provided, one positioning convex ring is also provided. When the number of the PCR reaction holes 210 is plural, the number of the positioning convex rings may be equal to the number of the PCR reaction holes 210, i.e., one positioning convex ring is disposed on the periphery of one PCR reaction hole 210, or the number of the positioning convex rings may be smaller than the number of the PCR reaction holes 210, and one large positioning convex ring surrounds all the PCR reaction holes 210.

It should be noted that, the first sealing portion 220 may be in the form of grooves, patterns, or buckles, in addition to the protruding form. For example, the first sealing part 220 may be an annular groove formed on the top surface 201 of the PCR kit, and an annular protrusion matching with the annular groove is formed on the outer bottom surface of the heat conductive cylinder 110.

The top surface 201 of the PCR kit and the top end of the PCR reaction well 210 are provided with chamfer surfaces 211. The chamfer 211 may facilitate the entry of the sealing post 120 into the PCR reaction well 210. Wherein, the outer diameter of the sealing ring 130 is larger than the aperture of the PCR reaction hole 210 and is also larger than the maximum aperture of the chamfer 211. So set up, sealing washer 130 can press from both sides between the outer bottom surface of heat conduction section of thick bamboo 110 and the top surface 201 of PCR kit, can not get into in the PCR reaction hole 210 to can reduce the soft rubber material direct contact PCR reaction hole 210 inside bring prevent adverse effect such as comdenstion water effect bad.

In an operation process, when the PCR instrument thermal cover 100 is sealed at the PCR reaction hole 210, the fixing convex ring 122 of the sealing post 120 contacts with the inner sidewall of the PCR reaction hole 210 to form a first seal; the sealing ring 130 is arranged to form a second seal; the first sealing part 220 contacts with the outer bottom surface of the heat conductive cylinder 110 to form a third sealing. Therefore, the thermal cover 100 of the PCR instrument has three sealing protection parts, has better sealing effect and eliminates the problem of aerosol scattering.

The top surface 201 of the PCR kit is provided with at least one thermal cover storage hole 230. Wherein, when 1 PCR reaction well 210 is provided, one thermal cover storage well 230 is also provided. When the number of the plurality of the PCR reaction wells 210 is equal to the number of the plurality of the heat-cover storage wells 230, that is, one heat-cover storage well 230 stores the heat-cover 100 of the PCR apparatus. Alternatively, the number of the thermal cover storage holes 230 may be smaller than the number of the PCR reaction holes 210, i.e., one thermal cover storage hole 230 stores one large PCR instrument thermal cover 100 for multi-hole sealing, or one thermal cover storage hole 230 stores a plurality of PCR instrument thermal covers 100 stacked layer by layer for single-hole sealing.

In this embodiment, the thermal cover storage hole 230 is a step hole, and the bottom end of the step hole is closed, so that pollution to the outside of the PCR kit can be avoided. The thermal cover storage hole 230 includes a first hole 231 and a second hole 232 connected in sequence from top to bottom, the aperture of the first hole 231 being larger than the aperture of the second hole 232; the first hole 231 has a size greater than or equal to the size of the flange 113, and the second hole 232 has a hole diameter greater than the outer diameter of the sealing post 120 and less than the minimum outer diameter of the rim 112. The inner wall of the first hole 231 is convexly provided with a limiting bump 233, and the limiting bump 233 is used for corresponding to the notch 114 and the limiting raised line 117 to realize circumferential limitation.

In an operation, when the PCR instrument thermal cover 100 is placed in the thermal cover storage hole 230, the step surface of the thermal cover storage hole 230 is used as a placement surface to be in contact with the outer bottom surface of the heat conductive cylinder 110; the outer surface of the limit bump 233 is used as a positioning surface, and is in contact with the surface of the notch 114 of the flange 113 and the outer surface of the limit protrusion 117. Due to the arrangement of the placement surface and the positioning surface, the contact area between the thermal cover 100 and the thermal cover storage hole 230 of the PCR instrument can be reduced, pollution is avoided, and the thermal cover 100 of the PCR instrument can be better stored in the thermal cover storage hole 230.

Referring to fig. 8, a schematic structural diagram of a thermal cover removing device 300 according to an embodiment of the disclosure is shown. Referring to fig. 9, a front view of a thermal cover removing device 300 according to an embodiment of the present application is shown. Referring to fig. 10, a cross-sectional view of fig. 9 in the direction C-C is shown according to an embodiment of the present application. The thermal cover removing device 300 can be used in combination with the thermal cover 100 of the PCR apparatus according to any one of the embodiments shown in FIGS. 1 to 5.

The thermal cover removing and detaching device 300 includes: at least one heating column 340, heating rod or heating plate and other devices for heating may be arranged in the heating column 340, the outer sleeve of the heating column 340 may be made of material with better heat conduction performance, and the outer side wall of the heating column 340 is provided with a fixing slot 341 matched with the elastic buckle 116. The fixing clip groove 341 may be any one of a square groove, a bar groove, and an annular groove.

The thermal cover removing device 300 further includes: the base plate 310, the taking-off and taking-off moving plate 320, the first driving mechanism 370 and at least one cover-off top piece 330, wherein the heating column 340 is connected to the base plate 310 and penetrates through the opening groove 321, and the taking-off and taking-off moving plate 320 is provided with at least one U-shaped, semicircular, circular, elliptic or square opening groove 321; the cover-unloading top sheet 330 is connected to the unloading moving plate 320 and penetrates through the opening groove 321; the first driving mechanism 370 is disposed on the base plate 310 and connected to the unloading moving plate 320, for driving the unloading moving plate 320 to move along the axis of the heating column 340.

In an operation process, when the PCR instrument thermal cover 100 needs to be heated, the PCR instrument thermal cover 100 is only required to be sleeved on the heating column 340 and fixed by the elastic buckle 116 and the fixing slot 341. When the thermal cover 100 of the PCR instrument needs to be disassembled, the removable moving plate 320 is driven by the first driving mechanism 370 to move downward along the axis of the heating column 340, the removable top sheet 330 is inserted between the heating column 340 and the thermal cover 100 of the PCR instrument, and gives a force to the elastic buckle 116, so that the elastic buckle 116 is separated from the fixing slot 341, and the thermal cover 100 of the PCR instrument on the heating column 340 is disassembled, and then the thermal cover 100 of the PCR instrument can be sealed in the reaction hole of the PCR test tube, the 8-hole plate, the 96-hole plate or the reagent box.

Therefore, the thermal cover 100 and the heating column 340 of the PCR instrument of the present embodiment can be detachably connected through the elastic buckle 116 and the fixing slot 341, and the thermal cover 100 of the PCR instrument on the heating column 340 can be automatically detached through the first driving mechanism 370, thereby improving the degree of automation and the working efficiency.

The number of heating columns 340 is determined by the number of heat transfer tubes 110 and the number of PCR instrument heat covers 100. The number of cover removal topsheets 330 is determined by the number of elastic snaps 116. The number of the open grooves 321 is determined by the number of the thermal covers 100 of the PCR instrument, and may be larger than the number of the thermal covers 100 of the PCR instrument, for example, other things such as a suction head may be detached while the thermal covers are detached.

In the present embodiment, the first driving mechanism 370 includes: the electric cylinder 371 is provided with a first perforation 311 on the base plate 310, the electric cylinder 371 is arranged on the base plate 310, and a main shaft of the electric cylinder 371 penetrates through the first perforation 311 to be connected with the taking-off moving plate 320 for driving the taking-off moving plate 320 to move up and down. In other embodiments, the first drive mechanism 370 may include one or more of a pulley drive assembly, a gear drive assembly, a cam drive assembly, a cylinder drive assembly, a hydraulic cylinder drive assembly, and a motor lead screw drive assembly.

At least one second through hole 312 is formed in the base plate 310, and at least one guide rod 372 is connected to the removal motion plate 320, and each guide rod 372 is inserted into one second through hole 312. In this embodiment, two second through holes 312 are provided, which are respectively located at two sides of the first through hole 311, and two guide rods 372 are provided. The present embodiment guides the up-and-down movement of the take-off moving plate 320 through the guide rod 372, so that the movement of the take-off moving plate 320 is stabilized.

The thermal cover removing device 300 further includes: the transfer post 350 and the heat insulation pad 360, the transfer post 350 is disposed between the heating post 340 and the base plate 310. The insulating spacer 360 is disposed between the adapter posts 350 and the base plate 310. The connection mode between the transfer post 350 and the heating post 340, the connection mode between the heat insulation pad 360 and the transfer post 350, and the connection mode between the heat insulation pad 360 and the substrate 310 can be bolt connection or buckle connection, etc.

The switching post 350 may be provided with a temperature switch, a temperature sensor, and other devices, for controlling the opening and closing of the heating post 340, and for detecting the temperature of the heating post 340, and the outer jacket of the switching post 350 may be made of a material with good heat conductivity. By providing devices such as a temperature switch and a temperature sensor within the adapter post 350, the size of the heating post 340 can be reduced. The heat insulating blanket 360 may be made of a material having superior heat insulating properties for insulating the heating column 340 from heat transfer to other places where heat energy is not required.

Referring to fig. 11, a schematic structural diagram of a thermal cover removing device 300 according to an embodiment of the disclosure is shown. The thermal cover removing device 300 further includes: the second driving mechanism 380 is in transmission connection with the base plate 310, and is used for driving the base plate 310 to move, so that components such as the heating column 340 and the like can be driven to move together. The second driving mechanism 380 may be a two-dimensional motion system or a three-dimensional motion system, and may include one or more of a pulley transmission assembly, a gear transmission assembly, a cam transmission assembly, a cylinder transmission assembly, a hydraulic cylinder transmission assembly, and a motor screw transmission assembly.

The thermal cover removing device 300 may further include a main control unit, where the main control unit is connected to the adaptor, the second driving mechanism 380, and the first driving mechanism 370 for control.

In an operation process, first, the second driving mechanism 380 drives the components such as the substrate 310 and the heating column 340 to move laterally above the thermal cover storage hole 230; then the second driving mechanism 380 drives the heating column 340 to move downwards, the heating column 340 is combined with the PCR instrument heat cover 100 through the tensioning friction force, at the moment, the elastic buckle 116 is clamped in the fixing clamping groove 341, and the PCR instrument heat cover 100 is not easy to fall off from the heating column 340; then, the second driving mechanism 380 drives the heating column 340 to move upwards, and drives the PCR instrument heat cover 100 sleeved on the heating column 340 to leave.

Then, the heating column 340 is opened by the temperature switch in the switching column 350 to heat the PCR instrument heat cover 100, and when the temperature sensor in the switching column 350 detects that the temperature of the heating column 340 reaches the preset value, the heating of the PCR instrument heat cover 100 is stopped by the temperature switch in the switching column 350.

Then, the second driving mechanism 380 drives the heating column 340 and other components to move transversely to the upper side of the PCR reaction hole 210, and aligns the sealing column 120 and the PCR reaction hole 210; then, the first driving mechanism 370 drives the removing moving plate 320 to move downwards, the removing moving plate 320 is propped against the flange 113 of the thermal cover 100 of the PCR instrument, the thermal cover top sheet arranged on the removing moving plate 320 firstly pushes the elastic buckle 116 outwards, so that the elastic buckle 116 does not limit the thermal cover 100 of the PCR instrument any more, the first driving mechanism 370 drives the removing moving plate 320 to move downwards continuously, the thermal cover 100 of the PCR instrument is extruded to be separated from the heating column 340, and falls down to the position of the PCR reaction hole 210, and the sealing of the PCR reaction hole 210 is realized.

It should be noted that, without conflict, features in the embodiments of the present application may be combined with each other.

The foregoing is merely a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and variations may be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. The PCR kit is characterized by being used in cooperation with a PCR instrument heat cover, wherein the PCR instrument heat cover comprises at least one heat conduction cylinder, at least one connecting hole is formed in the inner side wall of each heat conduction cylinder, and at least one elastic buckle is connected to the inner side wall of each connecting hole;

the heat conduction device comprises a heat conduction cylinder, a heat conduction pipe, a sealing ring, a central hole, a fixing convex ring, a sealing ring and a sealing ring, wherein the outer bottom surface of the heat conduction cylinder is convexly provided with at least one sealing post, the sealing post is provided with the central hole, the side wall of one end of the sealing post far away from the heat conduction cylinder is provided with the fixing convex ring, the sealing post is sleeved with the sealing ring, and the sealing ring is arranged between the fixing convex ring and the heat conduction cylinder;

the heat conduction cylinder comprises a chassis and a frame, wherein the bottom end of the frame is connected to the outer edge of the chassis, the top end of the frame is provided with an opening, and a flanging is outwards protruded; the flanging is provided with a notch;

at least one limit raised line is arranged on the side wall of the heat conduction cylinder, and the limit raised line extends to the flanging;

at least one PCR reaction hole is formed in the top surface of the PCR kit, a first sealing part is arranged on the top surface of the PCR kit and positioned at the PCR reaction hole, and the first sealing part is configured to be in sealing fit with the thermal cover of the PCR instrument.

2. The PCR kit of claim 1, wherein the first sealing portion includes a positioning collar for face sealing with an outer bottom surface of the thermally conductive cartridge.

3. The PCR kit of claim 1, wherein the top surface of the PCR kit is provided with at least one thermal cover storage well.

4. The PCR kit as claimed in claim 3, wherein the thermal cover storage hole is a stepped hole, the thermal cover storage hole includes a first hole and a second hole connected to each other, the aperture of the first hole is larger than that of the second hole, and a limit bump is protruded on an inner wall of the first hole.

5. The hot cover removing device is characterized by being used for being matched with the hot cover of the PCR instrument, wherein the hot cover of the PCR instrument comprises at least one heat conduction cylinder, at least one connecting hole is formed in the inner side wall of each heat conduction cylinder, and at least one elastic buckle is connected to the inner side wall of each connecting hole;

the hot cover taking and unloading device comprises:

a substrate;

the unloading moving plate is provided with at least one open slot;

at least one cover-unloading top piece connected to the removing and unloading motion plate and penetrating into the opening groove;

at least one heating column connected to the base plate and penetrating the opening groove, wherein a fixing clamping groove matched with the elastic buckle is formed in the outer side wall of the heating column; and

the first driving mechanism is arranged on the base plate, connected with the taking-off and unloading moving plate and used for driving the taking-off and unloading moving plate to move along the axis of the heating column.

6. The thermal cover removal device of claim 5, further comprising:

the switching column is arranged between the heating column and the substrate; and

the heat insulation base plate is arranged between the transfer column and the base plate.

7. The thermal cover removal device of claim 5, further comprising:

and the second driving mechanism is in transmission connection with the substrate and is used for driving the substrate to move.