CN113832023A - PCR micro-fluidic temperature control device - Google Patents
PCR micro-fluidic temperature control device Download PDFInfo
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- CN113832023A CN113832023A CN202111258797.1A CN202111258797A CN113832023A CN 113832023 A CN113832023 A CN 113832023A CN 202111258797 A CN202111258797 A CN 202111258797A CN 113832023 A CN113832023 A CN 113832023A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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- B01L2200/02—Adapting objects or devices to another
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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Abstract
The invention discloses a PCR microfluidic temperature control device which comprises a machine body, a heating assembly and a lifting assembly, wherein the machine body is provided with an installation space, the heating assembly comprises a heating table and a pressing plate, the pressing plate and the heating table are movably arranged in the installation space and are oppositely arranged, the heating table is used for containing and heating a reagent card, and the lifting assembly is connected with the heating table and/or the pressing plate so as to drive the heating table and/or the pressing plate to enable the heating table and the pressing plate to be close to or far away from each other so as to compress or release the reagent card. The PCR microfluidic temperature control device has the advantages of effectively reducing heat loss, realizing the tight adhesion between an amplification area of a reagent disc and a heat conducting plate and improving the temperature rise and reduction rate of the reagent disc.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a PCR microfluidic temperature control device.
Background
Polymerase Chain Reaction (PCR) (polymerase Chain reaction) is short, and PCR is a method for synthesizing a specific DNA fragment in vitro enzymatically, and relates to the application of a microfluidic device, namely the microfluidic device enables a reaction solution reagent to be subjected to periodic cycle treatment through steps of heating at different temperatures and the like at different stages, so that the target DNA can be rapidly amplified. The PCR has the characteristics of strong specificity, high sensitivity, simple and convenient operation, time saving and the like; it can be used not only for basic research of gene separation, cloning and nucleic acid sequence analysis, but also for diagnosis of diseases.
Microfluidics (Microfluidics) refers to the manipulation of liquids on a sub-millimeter scale, typically in the range of a few microns to hundreds of microns. The microfluidics integrates basic operation units related to the biological and chemical fields, even the whole assay operation, including sampling, dilution, reaction, separation, detection and the like, on a small Chip, so the microfluidic Chip is also called a lab-a-Chip (Labon-a-Chip), which can not only simplify the operation steps, shorten the detection time, save the reagent consumption, but also improve the accuracy and sensitivity of the detection.
In order to meet clinical requirements and shorten sample detection time, the temperature rise and fall rate, temperature consistency and heat transfer efficiency of a system need to be controlled. In the related art, the heating block and the reagent disk are usually set to be in a relative rotation structure, so that sliding friction is generated between the heating block and the reagent disk, the surface of the disk is abraded, the optical detection accuracy is affected, meanwhile, air heat transfer is adopted, so that heating is not uniform, the temperatures of different areas cannot be quickly consistent, and the temperature control is difficult to guarantee.
Disclosure of Invention
The invention mainly aims to provide a PCR microfluidic temperature control device, and aims to provide a PCR microfluidic temperature control device which is low in heat loss, high in temperature rise and reduction rate and simple in structure.
In order to achieve the above object, the present invention provides a PCR microfluidic temperature control device, comprising:
a body provided with an installation space;
the heating assembly comprises a heating table and a pressing plate, the pressing plate and the heating table are movably arranged in the mounting space and are oppositely arranged, and the heating table is used for containing and heating reagent cards; and
the lifting assembly is connected with the heating table and/or the pressing plate so as to drive the heating table and/or the pressing plate to move, so that the heating table and the pressing plate are close to or far away from each other to compress or release the reagent card.
In one embodiment, the heating table is provided with a constant temperature area and a variable temperature area which are arranged at intervals;
the pressing plate comprises a pressing portion and foam, the pressing portion and the foam are movably arranged in the installation space, the pressing portion is opposite to the variable-temperature area, and the foam is arranged on one side, facing the variable-temperature area, of the pressing portion.
In one embodiment, the heating station comprises:
the base is movably arranged in the mounting space;
the temperature changing block is arranged on the base and corresponds to the pressing plate so as to form the temperature changing area; and
the constant temperature block is arranged on the base and is spaced from the temperature changing block to form the constant temperature area.
In one embodiment, the temperature change block comprises:
the first heat insulation block is arranged on the base and corresponds to the pressing plate, and a first mounting groove is formed in one side, back to the base, of the first heat insulation block;
the refrigerator is arranged in the first mounting groove; and
the first heat-conducting plate is arranged on the first heat-insulating block to cover the notch of the first mounting groove.
In one embodiment, the temperature change block further comprises a heat sink and a heat dissipation fan, the heat sink is disposed on a side of the first thermal insulation block facing away from the first thermal conductive plate, the base is provided with a via hole corresponding to the heat sink, and the heat dissipation fan is disposed on a side of the base facing away from the temperature change block and corresponding to the heat sink;
and/or, the temperature changing block further comprises a first protection switch and a first temperature sensor, the first protection switch is electrically connected with the first temperature sensor, the first heat insulation block is provided with a first accommodating groove and a second accommodating groove at intervals, the first protection switch is arranged in the first accommodating groove, and the first temperature sensor is arranged in the second accommodating groove and is abutted to the first heat conducting plate;
and/or, the refrigerator includes a plurality of, the first heat insulation block is provided with a plurality of the first mounting grooves, and each refrigerator is arranged in one of the first mounting grooves;
and/or heat-conducting silicone grease is filled between the first heat-conducting plate and the refrigerator.
In one embodiment, the thermostatic block comprises:
the second heat insulation block is arranged on the base and is positioned at one end, far away from the temperature change block, of the base, and a second mounting groove is formed in one side, back to the base, of the second heat insulation block;
the heating film is arranged in the second mounting groove; and
the second heat-conducting plate is arranged on the second heat-insulating block to cover the notch of the second mounting groove.
In an embodiment, the constant temperature block further includes a second protection switch and a second temperature sensor, the second protection switch is electrically connected to the second temperature sensor, the second heat insulation block is provided with a first accommodating groove and a second accommodating groove at an interval, the second protection switch is disposed in the first accommodating groove, and the second temperature sensor is disposed in the second accommodating groove and abutted to the second heat conduction plate;
and/or heat-conducting silicone grease is filled between the second heat-conducting plate and the heating film.
In one embodiment, the machine body includes a first fixing plate and a second fixing plate, and the first fixing plate and the second fixing plate are disposed opposite to each other and spaced apart from each other to enclose the installation space;
the pressing plate is connected with the first fixing plate in a sliding mode, and the heating table is connected with the second fixing plate in a sliding mode.
In one embodiment, the lift assembly comprises:
the output shaft of the driving part is connected with the heating table;
the two synchronizing wheels are arranged on the first fixing plate at intervals;
the synchronous belt is sleeved on the two synchronous wheels, and the heating table and the pressing plate are connected with the synchronous belt;
wherein, the driving piece drive when the warm table removes, the warm table drives the hold-in range removes, so that the warm table with the clamp plate is close to each other or keeps away from.
In one embodiment, one of the pressure plate and the first fixing plate is provided with a first sliding table, and the other of the pressure plate and the first fixing plate is provided with a first sliding groove, and the first sliding groove is in sliding fit with the first sliding table;
and/or one of the heating table and the second fixing plate is provided with a second sliding table, and the other of the heating table and the second fixing plate is provided with a second sliding groove which is in sliding fit with the second sliding table;
and/or a connecting table is arranged at one end of the heating table, which is far away from the pressing plate, the connecting table comprises a sliding part and a connecting part which are arranged at an included angle, the sliding part is connected with the second fixing plate in a sliding manner, and the connecting part is connected with an output shaft of the driving part;
and/or, the hold-in range has first area section and second area section, first area section with the second area section is located two relative both sides of synchronizing wheel line, the clamp plate is equipped with first connecting seat, first connecting seat with first area section is connected, the heating station is close to the one end of clamp plate is equipped with the second connecting seat, the second connecting seat with the second area section is connected.
According to the PCR microfluidic temperature control device, the heating assembly is arranged in the installation space of the machine body, the pressing plate and the heating table of the heating assembly are movably arranged in the installation space of the machine body and are oppositely arranged, so that the lifting assembly is connected with the heating table and/or the pressing plate to drive the heating table and/or the pressing plate to move, the heating table and the pressing plate are close to each other, the reagent card on the heating table is pressed by the pressing plate, air flow on the upper surface of the amplification area of the reagent card can be reduced, heat loss is effectively reduced, meanwhile, the reagent card is mutually pressed by the heating table and the pressing plate, the adhesion degree of the amplification area of the reagent card and a heat conducting plate of the heating table is realized, and the temperature rising and falling rates of the reagent card are improved. When the operation is finished, the heating table and/or the pressing plate are driven to move by the lifting assembly, so that the heating table and the pressing plate are far away from each other to release the reagent card.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a PCR microfluidic temperature control device according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a heating stage according to an embodiment of the present invention;
FIG. 3 is a schematic view of a portion of a heating stage according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a thermostatic block according to an embodiment of the present invention;
FIG. 5 is a schematic view of a portion of a thermostatic block according to an embodiment of the present invention;
FIG. 6 is a schematic structural diagram of a temperature change block in accordance with an embodiment of the present invention;
fig. 7 is a schematic partial structure diagram of a temperature change block according to an embodiment of the invention.
The reference numbers illustrate:
| reference numerals | Name (R) | Reference numerals | Name (R) |
| 100 | PCR micro-fluidic |
2311 | Second mounting groove |
| 1 | Machine body | 2312 | A first accommodation groove |
| 11 | |
2313 | |
| 12 | |
232 | Heating film |
| 13 | |
233 | Second heat conducting plate |
| 14 | First sliding table | 234 | Second |
| 15 | Second sliding table | 235 | |
| 2 | Heating table | 24 | Connecting table |
| 21 | |
241 | Sliding |
| 22 | |
242 | Connecting |
| 221 | First |
243 | |
| 2211 | First mounting groove | 25 | Second connecting |
| 2212 | First holding tank | 3 | |
| 2213 | Second holding tank | 31 | |
| 222 | Refrigerating device | 32 | |
| 223 | First heat conducting plate | 33 | First connecting |
| 224 | |
4 | |
| 225 | First protection switch | 41 | |
| 226 | First temperature sensor | 42 | Synchronizing |
| 23 | Constant temperature block | 43 | |
| 231 | Second heat insulation block |
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that all the directional indicators (such as up, down, left, right, front, and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the movement situation, etc. in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly.
Also, the meaning of "and/or" and/or "appearing throughout is meant to encompass three scenarios, exemplified by" A and/or B "including scenario A, or scenario B, or scenarios where both A and B are satisfied.
In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Polymerase Chain Reaction (PCR) (polymerase Chain reaction) is short, and PCR is a method for synthesizing a specific DNA fragment in vitro enzymatically, and relates to the application of a microfluidic device, namely the microfluidic device enables a reaction solution reagent to be subjected to periodic cycle treatment through steps of heating at different temperatures and the like at different stages, so that the target DNA can be rapidly amplified. The PCR has the characteristics of strong specificity, high sensitivity, simple and convenient operation, time saving and the like; it can be used not only for basic research of gene separation, cloning and nucleic acid sequence analysis, but also for diagnosis of diseases.
Microfluidics (Microfluidics) refers to the manipulation of liquids on a sub-millimeter scale, typically in the range of a few microns to hundreds of microns. The microfluidics integrates basic operation units related to the biological and chemical fields, even the whole assay operation, including sampling, dilution, reaction, separation, detection and the like, on a small Chip, so the microfluidic Chip is also called a lab-a-Chip (Labon-a-Chip), which can not only simplify the operation steps, shorten the detection time, save the reagent consumption, but also improve the accuracy and sensitivity of the detection.
In order to meet clinical requirements and shorten sample detection time, the temperature rise and fall rate, temperature consistency and heat transfer efficiency of a system need to be controlled. In the related art, the heating block and the reagent disk are usually set to be in a relative rotation structure, so that sliding friction is generated between the heating block and the reagent disk, the surface of the disk is abraded, the optical detection accuracy is affected, meanwhile, air heat transfer is adopted, so that heating is not uniform, the temperatures of different areas cannot be quickly consistent, and the temperature control is difficult to guarantee.
Based on the above conception and problems, the present invention provides a PCR microfluidic temperature control device 100. It is understood that the PCR microfluidic temperature control device 100 is used for performing an assay operation on a sample on a reagent card.
Referring to fig. 1 to 3, in an embodiment of the present invention, the PCR microfluidic temperature control device 100 includes a body 1, a heating assembly and a lifting assembly 4, wherein the body 1 has an installation space 11, the heating assembly includes a heating stage 2 and a pressing plate 3, the pressing plate 3 and the heating stage 2 are movably disposed in the installation space 11 and are oppositely disposed, the heating stage 2 is used for accommodating and heating a reagent card, and the lifting assembly 4 is connected to the heating stage 2 and/or the pressing plate 3 to drive the heating stage 2 and/or the pressing plate 3 to move, so that the heating stage 2 and the pressing plate 3 are close to or away from each other to compress or release the reagent card.
In this embodiment, the machine body 1 is used to mount, fix or support the heating assembly, the lifting assembly 4 and other components of the PCR microfluidic temperature control device 100, that is, the machine body 1 provides a mounting base for the heating assembly, the lifting assembly 4 and other components of the PCR microfluidic temperature control device 100. It can be understood that the machine body 1 may be a mounting frame, a mounting shell, a rack, a machine table, a machine body, a mounting table, a mounting plate, or the like, and is not limited herein. Of course, in order to make the heating assembly, the lifting assembly 4, and other components of the PCR microfluidic temperature control device 100 cooperate with each other, the machine body 1 may further be provided with a step structure or a gantry, which is not limited herein.
It can be understood that the heating assembly is arranged into a heating table 2 and a pressing plate 3, so that the heating table 2 is used for containing and heating the reagent card, the reagent card can be pressed by the pressing plate 3, the air flow on the upper surface of the amplification area of the reagent card is reduced, and the heat loss of the reagent card is effectively reduced; meanwhile, the reagent card is mutually compressed by the heating table 2 and the pressing plate 3, so that the adhesion degree of the reagent card amplification area and the heat-conducting plate of the heating table 2 is realized, and the temperature rise and fall rate of the reagent card is improved.
In the present embodiment, the lifting assembly 4 is arranged such that the lifting assembly 4 is connected with the heating stage 2 and/or the pressing plate 3 to drive the heating stage 2 and/or the pressing plate 3 to move, thereby enabling the heating stage 2 and the pressing plate 3 to approach each other to press the reagent card, or enabling the heating stage 2 and the pressing plate 3 to move away from each other to release the reagent card. It can be understood that the lifting assembly 4 may be a driving cylinder, a driving motor, a driving wheel cooperating with a synchronous belt, and the like, and is not limited herein.
It is understood that the lifting assembly 4 may include one or more, and the lifting assembly 4 may be connected to the heating stage 2 only to drive the heating stage 2 to move; alternatively, the lifting assembly 4 may be connected only to the platen 3 to drive the platen 3 to move; alternatively, the lifting assembly 4 is connected with the heating stage 2 and the pressing plate 3 to drive the heating stage 2 and the pressing plate 3 to move.
According to the PCR microfluidic temperature control device 100, the heating assembly is arranged in the installation space 11 of the machine body 1, the pressing plate 3 and the heating table 2 of the heating assembly are movably arranged in the installation space 11 of the machine body 1 and are oppositely arranged, so that the lifting assembly 4 is connected with the heating table 2 and/or the pressing plate 3 to drive the heating table 2 and/or the pressing plate 3 to move, the heating table 2 and the pressing plate 3 are close to each other, the reagent card on the heating table 2 is pressed by the pressing plate 3, the air flow on the upper surface of the amplification area of the reagent card can be reduced, the heat loss is effectively reduced, meanwhile, the reagent card is pressed by the heating table 2 and the pressing plate 3, the adhesion degree of the amplification area of the reagent card and the heat conducting plate of the heating table 2 is realized, and the temperature rising and falling rate of the reagent card is improved. When the operation is finished, the heating table 2 and/or the pressing plate 3 are driven to move by the lifting assembly 4, so that the heating table 2 and the pressing plate 3 are far away from each other to release the reagent card.
In one embodiment, as shown in fig. 1, the heating table 2 has a constant temperature area and a variable temperature area which are arranged at intervals; the pressing plate 3 comprises a pressing part 31 and foam 32 movably arranged in the installation space 11, the pressing part 31 is opposite to the temperature-changing zone, and the foam 32 is arranged on one side of the pressing part 31 facing the temperature-changing zone.
It can be understood that, by arranging the constant temperature area and the variable temperature area on the heating platform 2, the constant temperature area is used for providing a constant temperature heat source for the extraction process of the sample on the reagent card, and the variable temperature area is used for providing temperature circulation for three stages of denaturation, annealing and extension in the amplification of the sample on the reagent card.
In this embodiment, through setting up clamp plate 3 into compressing tightly portion 31 and bubble cotton 32 for compressing tightly portion 31 can compress tightly the reagent card, utilizes bubble cotton 32 to realize keeping warm to the reagent card, with effective reduction heat loss. It is understood that the compressing portion 31 may be a plate-shaped structure, and the compressing portion 31 may be made of plastic, wood, or metal, and is not limited herein.
In one embodiment, the heating platform 2 includes a base 21, a temperature changing block 22 and a constant temperature block 23, wherein the base 21 is movably disposed in the installation space 11, the temperature changing block 22 is disposed on the base 21 and corresponds to the pressure plate 3 to form a temperature changing area, and the constant temperature block 23 is disposed on the base 21 and is spaced apart from the temperature changing block 22 to form a constant temperature area.
It can be understood that, by arranging the temperature-changing block 22 and the temperature-keeping block 23 on the base 21, the temperature-changing block 22 corresponds to the pressure plate 3, so that the pressure plate 3 can be used to press the reagent card onto the temperature-changing block 22, thereby smoothly amplifying the sample of the reagent card. In this embodiment, the temperature change block 22 can provide a temperature change in the range of 40 ℃ to 100 ℃ to suit the requirements of different stages of sample amplification of the reagent card. The thermostatic block 23 may provide a temperature environment of 105 ℃.
In the present embodiment, as shown in fig. 1 to 7, the temperature change block 22 includes a first thermal insulation block 221, a refrigerator 222 and a first thermal conductive plate 223, wherein the first thermal insulation block 221 is disposed on the base 21 and disposed corresponding to the pressing plate 3, a first installation groove 2211 is disposed on a side of the first thermal insulation block 221 facing away from the base 21, the refrigerator 222 is disposed in the first installation groove 2211, and the first thermal conductive plate 223 is disposed on the first thermal insulation block 221 and abuts against the refrigerator 222 to cover a notch of the first installation groove 2211.
In the present embodiment, the refrigerator 222 is disposed in the first mounting groove 2211 of the first heat insulation block 221, so that the first heat insulation block 221 is utilized to insulate the base 21 and limit the refrigerator 222, and the heat loss of the refrigerator 222 is effectively reduced. It can be understood that, by arranging the first heat conduction plate 223, the first heat conduction plate 223 is conveniently utilized to realize rapidly transferring heat, and the first heat conduction plate 223 is utilized to realize the temperature uniformity of the reagent card, so as to improve the accuracy.
Optionally, a heat conductive silicone grease is filled between the first heat conductive plate 223 and the refrigerator 222, and this arrangement can further improve the heat conduction efficiency. In this embodiment, the refrigerator 222 includes a plurality of refrigerators, the first insulation block 221 is provided with a plurality of first installation grooves 2211, and each refrigerator 222 is installed in one first installation groove 2211. Optionally, the refrigerator 222 is a TEC (semiconductor refrigerator Thermo Electric Cooler). Optionally, the first heat conducting plate 223 is an aluminum plate, and the first heat insulating block 221 is made of an epoxy resin material.
In an embodiment, as shown in fig. 1, 6 and 7, the temperature change block 22 further includes a heat sink 224 and a heat dissipation fan, the heat sink 224 is disposed on a side of the first thermal insulation block 221 facing away from the first thermal conductive plate 223, the base 21 is provided with a via hole corresponding to the heat sink 224, and the heat dissipation fan is disposed on a side of the base 21 facing away from the temperature change block 22 and corresponding to the heat sink 224. It will be appreciated that heat dissipation is achieved for the refrigerator 222 using the heat sink 224 and the heat dissipation fan to facilitate temperature change of the first heat conduction plate 223.
In an embodiment, as shown in fig. 1, 2, 3, 6 and 7, the temperature change block 22 further includes a first protection switch 225 and a first temperature sensor 226, the first protection switch 225 is electrically connected to the first temperature sensor 226, the first insulation block 221 is provided with a first accommodating groove 2212 and a second accommodating groove 2213 at intervals, the first protection switch 225 is disposed in the first accommodating groove 2212, and the first temperature sensor 226 is disposed in the second accommodating groove 2213 and abuts against the first heat conduction plate 223.
It can be understood that the temperature change of the refrigerator 222 is effectively controlled by providing the first protection switch 225 and the first temperature sensor 226 so as to detect the temperature of the first heat conduction plate 223 in real time using the first temperature sensor 226. In this embodiment, the first protection switch 225 is electrically connected to the refrigerator 222, and the operating state of the refrigerator 222 can be controlled by the first protection switch 225 according to the detection of the first temperature sensor 226.
In this embodiment, the TEC is attached to the upper side of the first adiabatic block 221, and the heat sink 224 is attached to the lower side of the first adiabatic block 221, so that heat is transferred longitudinally in the first thermal conductive plate 223-TEC-heat sink 224, and heat is prevented from being transferred transversely to other components. It can be understood that, during heating, the TEC releases heat to transfer to a local area of the first heat conducting plate 223, and due to the strong heat conducting property of the first heat conducting plate 223, the TEC rapidly diffuses to the whole area with a small temperature difference, and the reagent card is placed on the first heat conducting plate 223 to be heated.
In one embodiment, as shown in fig. 1 to 5, the thermostatic block 23 includes a second thermal insulation block 231, a heating film 232 and a second thermal conductive plate 233, wherein the second thermal insulation block 231 is disposed on the base 21 and located at an end of the base 21 away from the temperature changing block 22, a second mounting groove 2311 is disposed on a side of the second thermal insulation block 231 facing away from the base 21, the heating film 232 is disposed in the second mounting groove 2311, and the second thermal conductive plate 233 is disposed on the second thermal insulation block 231 and abuts against the heating film 232 to cover a notch of the second mounting groove 2311.
In this embodiment, the heating film 232 is disposed in the second mounting groove 2311 of the second heat insulation block 231, so that the second heat insulation block 231 is utilized to insulate the base 21 and limit the heating film 232, and the heat loss of the heating film 232 is effectively reduced. It can be understood that, by providing the second heat-conducting plate 233, the second heat-conducting plate 233 is conveniently utilized to realize rapid heat transfer, and the second heat-conducting plate 233 is utilized to realize temperature uniformity of the reagent card, so as to improve accuracy.
Optionally, a heat-conducting silicone grease is filled between the second heat conducting plate 233 and the heating film 232, so that the heat conduction efficiency can be further improved. In the present embodiment, the heating film 232 is a resistance wire heating film. Optionally, the second heat conducting plate 233 is an aluminum plate, and the second heat insulating block 231 is made of an epoxy resin material.
It is understood that the heating film 232 may be attached to the second heat conduction plate 233 and/or the second thermal insulation block 231 by 3M glue. In the present embodiment, the second installation groove 2311 of the second insulation block 231 also performs a thermal cover function.
In this embodiment, the temperature-changing block 22 is placed below the PCR chamber, and the TEC controls denaturation (95 ℃), annealing, and extension (60 ℃) of the PCR chamber reagents to achieve PCR amplification reaction, and the constant temperature block 23 is heated by the heating film 232 to maintain 105 ℃, and covers other chambers connected to the PCR chamber, so that a temperature difference is formed between 105 ℃ in the constant temperature region of the heating stage 2 and the temperature-changing region, thereby avoiding high temperature and high pressure in other chambers connected to the PCR, reducing evaporation of the liquid in the PCR chamber while suppressing precipitation of internal bubbles, reducing interference of factors on optical detection, and achieving a hot-cover function in the reagent card.
In an embodiment, as shown in fig. 1 to 5, the thermostatic block 23 further includes a second protection switch 234 and a second temperature sensor 235, the second protection switch 234 is electrically connected to the second temperature sensor 235, the second heat insulation block 231 is provided with a first receiving groove 2312 and a second receiving groove 2313 at an interval, the second protection switch 234 is disposed in the first receiving groove 2312, and the second temperature sensor 235 is disposed in the second receiving groove 2313 and abuts against the second heat conduction plate 233.
It can be understood that the temperature of the second heat conductive plate 233 is detected in real time by the second temperature sensor 235 by providing the second protection switch 234 and the second temperature sensor 235, thereby effectively controlling the temperature change of the heating film 232. In this embodiment, the second protection switch 234 is electrically connected to the heating film 232, and the operating state of the heating film 232 can be controlled by the second protection switch 234 according to the detection condition of the second temperature sensor 235.
In this embodiment, the heating film 232 is connected above the second heat insulation block 231, so that heat is longitudinally transferred in the second heat conduction plate 233-the heating film 232, and heat is insulated from being transversely transferred to other parts. It can be understood that, during heating, the heating film 232 releases heat to transfer to a local area of the second heat conducting plate 233, and due to the strong heat conducting property of the second heat conducting plate 233, the reagent card is rapidly diffused to the whole area with a small temperature difference, and is placed on the second heat conducting plate 233 for heating.
In one embodiment, as shown in fig. 1, the machine body 1 includes a first fixing plate 12 and a second fixing plate 13, the first fixing plate 12 and the second fixing plate 13 are disposed opposite to each other and spaced apart from each other to enclose a mounting space 11; the pressing plate 3 is connected with the first fixing plate 12 in a sliding manner, and the heating table 2 is connected with the second fixing plate 13 in a sliding manner.
In the present embodiment, the pressure plate 3 and the first fixing plate 12 are slidably connected, so that the first fixing plate 12 provides a guiding sliding function for the movement of the pressure plate 3 when the pressure plate 3 is driven to move by the lifting assembly 4. By arranging the heating table 2 and the second fixing plate 13 to be in sliding connection, the second fixing plate 13 provides a guiding sliding effect for the movement of the heating table 2 when the lifting assembly 4 drives the heating table 2 to move.
In one embodiment, one of the pressing plate 3 and the first fixing plate 12 is provided with a first sliding table 14, and the other of the pressing plate and the first fixing plate is provided with a first sliding groove, and the first sliding groove is in sliding fit with the first sliding table 14. One of the heating table 2 and the second fixing plate 13 is provided with a second sliding table 15, and the other of the heating table 2 and the second fixing plate 13 is provided with a second sliding groove 243, wherein the second sliding groove 243 is in sliding fit with the second sliding table 15.
In this embodiment, as shown in fig. 1, the first sliding groove and the first sliding table 14 are respectively disposed on the pressing plate 3 and the first fixing plate 12, and the first sliding groove is in sliding fit with the first sliding table 14, so that the first sliding table 14 provides sliding guide for the movement of the pressing plate 3, and the phenomenon that the pressing plate 3 is jammed in the movement is avoided. It can be understood that, by providing the second sliding groove 243 and the second sliding table 15 on the heating table 2 and the second fixing plate 13, the second sliding groove 243 and the second sliding table 15 are in sliding fit, so that the second sliding table 15 provides sliding guide for the movement of the heating table 2, and the phenomenon of jamming of the heating table 2 in the movement is avoided.
In an embodiment, as shown in fig. 1, a connecting table 24 is disposed at an end of the heating table 2 away from the pressing plate 3, the connecting table 24 includes a sliding portion 241 and a connecting portion 242, the sliding portion 241 is slidably connected to the second fixing plate 13, and the connecting portion 242 is connected to an output shaft of the lifting assembly 4.
It will be appreciated that the connecting portion 242 of the connecting table 24 is positioned with the base 21 of the heating table 2 by pins to ensure the flatness of the installation of the heating table 2.
In an embodiment, the lifting assembly 4 includes a driving member 41, two synchronizing wheels 42 and a timing belt 43, wherein an output shaft of the driving member 41 is connected to the heating stage 2, the two synchronizing wheels 42 are spaced apart from each other and disposed on the first fixing plate 12, the timing belt 43 is sleeved on the two synchronizing wheels 42, and the heating stage 2 and the pressing plate 3 are connected to the timing belt 43; when the driving member 41 drives the heating stage 2 to move, the heating stage 2 drives the timing belt 43 to move, so that the heating stage 2 and the pressing plate 3 move close to or away from each other.
In this embodiment, the driving member 41 may be selected as a driving motor, an output shaft of the driving motor is disposed in a screw rod, for example, a linear screw motor, and the output shaft of the driving motor is in threaded connection with the connecting portion 242 of the connecting table 24, so that when the driving motor drives the output shaft to rotate, the connecting portion 242 of the connecting table 24 drives the base 21 of the heating table 2 to move along the second sliding table 15.
It can be understood that two synchronous wheels 42 are disposed on the first fixing plate 12, and the synchronous belt 43 is sleeved on the two synchronous wheels 42, so that the synchronous belt 43 has a first belt segment and a second belt segment, which are located on two opposite sides of a connecting line of the two synchronous wheels 42. Through being connected warm table 2 and clamp plate 3 with second area section and first area section respectively for when driving piece 41 drive connecting portion 242 drove base 21 of warm table 2 along second slip table 15 ascending, the second area section is ascending, and first area section is descending, so that clamp plate 3 drives clamp plate 3 and descends, thereby makes warm table 2 and clamp plate 3 be close to each other.
In this embodiment, as shown in fig. 1, the pressing plate 3 is provided with a first connecting seat 33, the first connecting seat 33 is connected with the first belt section, one end of the heating platform 2 adjacent to the pressing plate 3 is provided with a second connecting seat 25, and the second connecting seat 25 is connected with the second belt section.
When the linear screw motor of the PCR microfluidic temperature control device 100 of the present invention works, the screw rotates to drive the connecting portion 242 of the connecting table 24 to ascend along the second sliding table 15, drive the heating table 2 to ascend, and synchronously transmit the motion to the second belt segment of the synchronous belt 43, so that the second belt segment of the synchronous belt 43 ascends, the first belt segment descends, and drive the platen 3 to descend by means of the first belt segment, so that the temperature changing block 22 of the heating table 2 is attached to the lower surface of the reagent card, and the upper surface of the reagent card is attached to the compressing portion 31 through the foam 32 of the platen 3. The structure can increase the contact area between the lower surface of the reagent card and the heating table 2, reduce the heat dissipation area of the upper surface and increase the temperature rise and fall speed of the reagent card.
The above description is only an alternative embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. A PCR microfluidic temperature control device, comprising:
a body provided with an installation space;
the heating assembly comprises a heating table and a pressing plate, the pressing plate and the heating table are movably arranged in the mounting space and are oppositely arranged, and the heating table is used for containing and heating reagent cards; and
the lifting assembly is connected with the heating table and/or the pressing plate so as to drive the heating table and/or the pressing plate to move, so that the heating table and the pressing plate are close to or far away from each other to compress or release the reagent card.
2. The PCR microfluidic temperature control device according to claim 1, wherein the heating stage has a constant temperature region and a variable temperature region arranged at intervals;
the pressing plate comprises a pressing portion and foam, the pressing portion and the foam are movably arranged in the installation space, the pressing portion is opposite to the variable-temperature area, and the foam is arranged on one side, facing the variable-temperature area, of the pressing portion.
3. The PCR microfluidic temperature control device according to claim 2, wherein said heating stage comprises:
the base is movably arranged in the mounting space;
the temperature changing block is arranged on the base and corresponds to the pressing plate so as to form the temperature changing area; and
the constant temperature block is arranged on the base and is spaced from the temperature changing block to form the constant temperature area.
4. The PCR microfluidic temperature control device according to claim 3, wherein said temperature change block comprises:
the first heat insulation block is arranged on the base and corresponds to the pressing plate, and a first mounting groove is formed in one side, back to the base, of the first heat insulation block;
the refrigerator is arranged in the first mounting groove; and
the first heat-conducting plate is arranged on the first heat-insulating block to cover the notch of the first mounting groove.
5. The PCR microfluidic temperature control device according to claim 4, wherein the temperature varying block further comprises a heat sink and a heat dissipation fan, the heat sink is disposed on a side of the first thermal insulation block facing away from the first thermal conductive plate, the base is provided with a via hole corresponding to the heat sink, and the heat dissipation fan is disposed on a side of the base facing away from the temperature varying block and corresponding to the heat sink;
and/or, the temperature changing block further comprises a first protection switch and a first temperature sensor, the first protection switch is electrically connected with the first temperature sensor, the first heat insulation block is provided with a first accommodating groove and a second accommodating groove at intervals, the first protection switch is arranged in the first accommodating groove, and the first temperature sensor is arranged in the second accommodating groove and is abutted to the first heat conducting plate;
and/or, the refrigerator includes a plurality of, the first heat insulation block is provided with a plurality of the first mounting grooves, and each refrigerator is arranged in one of the first mounting grooves;
and/or heat-conducting silicone grease is filled between the first heat-conducting plate and the refrigerator.
6. The PCR microfluidic temperature control device according to claim 3, wherein said thermostatic block comprises:
the second heat insulation block is arranged on the base and is positioned at one end, far away from the temperature change block, of the base, and a second mounting groove is formed in one side, back to the base, of the second heat insulation block;
the heating film is arranged in the second mounting groove; and
the second heat-conducting plate is arranged on the second heat-insulating block to cover the notch of the second mounting groove.
7. The PCR microfluidic temperature control device according to claim 6, wherein the thermostatic block further comprises a second protection switch and a second temperature sensor, the second protection switch is electrically connected with the second temperature sensor, the second heat insulating block is provided with a first containing groove and a second containing groove at intervals, the second protection switch is arranged in the first containing groove, and the second temperature sensor is arranged in the second containing groove and is abutted against the second heat conducting plate;
and/or heat-conducting silicone grease is filled between the second heat-conducting plate and the heating film.
8. The PCR microfluidic temperature control device according to any one of claims 1 to 7, wherein the body comprises a first fixing plate and a second fixing plate, the first fixing plate and the second fixing plate are oppositely and spaced to enclose the installation space;
the pressing plate is connected with the first fixing plate in a sliding mode, and the heating table is connected with the second fixing plate in a sliding mode.
9. The PCR microfluidic temperature control device according to claim 8, wherein said lifting assembly comprises:
the output shaft of the driving part is connected with the heating table;
the two synchronizing wheels are arranged on the first fixing plate at intervals;
the synchronous belt is sleeved on the two synchronous wheels, and the heating table and the pressing plate are connected with the synchronous belt;
wherein, the driving piece drive when the warm table removes, the warm table drives the hold-in range removes, so that the warm table with the clamp plate is close to each other or keeps away from.
10. The PCR microfluidic temperature control device according to claim 9, wherein one of the pressing plate and the first fixing plate is provided with a first sliding table, and the other of the pressing plate and the first fixing plate is provided with a first sliding groove, and the first sliding groove is in sliding fit with the first sliding table;
and/or one of the heating table and the second fixing plate is provided with a second sliding table, and the other of the heating table and the second fixing plate is provided with a second sliding groove which is in sliding fit with the second sliding table;
and/or a connecting table is arranged at one end of the heating table, which is far away from the pressing plate, the connecting table comprises a sliding part and a connecting part which are arranged at an included angle, the sliding part is connected with the second fixing plate in a sliding manner, and the connecting part is connected with an output shaft of the driving part;
and/or, the hold-in range has first area section and second area section, first area section with the second area section is located two relative both sides of synchronizing wheel line, the clamp plate is equipped with first connecting seat, first connecting seat with first area section is connected, the heating station is close to the one end of clamp plate is equipped with the second connecting seat, the second connecting seat with the second area section is connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202111258797.1A CN113832023A (en) | 2021-10-26 | 2021-10-26 | PCR micro-fluidic temperature control device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111258797.1A CN113832023A (en) | 2021-10-26 | 2021-10-26 | PCR micro-fluidic temperature control device |
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| CN113832023A true CN113832023A (en) | 2021-12-24 |
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| CN114317213A (en) * | 2021-12-31 | 2022-04-12 | 东莞理工学院 | Culture dish temperature control device |
| CN115113662A (en) * | 2022-06-23 | 2022-09-27 | 中元汇吉生物技术股份有限公司 | Gene amplification instrument, control method thereof, and computer-readable storage medium |
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| CN213659271U (en) * | 2020-12-31 | 2021-07-09 | 重庆中元汇吉生物技术有限公司 | Temperature control unit, temperature control unit and temperature control device |
| CN216192355U (en) * | 2021-10-26 | 2022-04-05 | 中元汇吉生物技术股份有限公司 | PCR micro-fluidic temperature control device |
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| CN111004708A (en) * | 2019-12-24 | 2020-04-14 | 中国科学院长春光学精密机械与物理研究所 | PCR temperature cycle control method and rotary drive type PCR temperature cycle control device |
| CN213659271U (en) * | 2020-12-31 | 2021-07-09 | 重庆中元汇吉生物技术有限公司 | Temperature control unit, temperature control unit and temperature control device |
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