CN111229350A - Liquid cooling automatic PCR equipment - Google Patents
Liquid cooling automatic PCR equipment Download PDFInfo
- Publication number
- CN111229350A CN111229350A CN201910924351.4A CN201910924351A CN111229350A CN 111229350 A CN111229350 A CN 111229350A CN 201910924351 A CN201910924351 A CN 201910924351A CN 111229350 A CN111229350 A CN 111229350A
- Authority
- CN
- China
- Prior art keywords
- liquid
- translation
- pcr
- lifting
- liquid cooling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Abstract
The invention relates to PCR amplification equipment, in particular to liquid cooling automatic PCR equipment. It includes: the liquid cooling heat dissipation mechanism and the automatic PCR amplification mechanism are arranged on the base; the liquid cooling heat dissipation mechanism comprises a refrigerating sheet, a liquid cooling plate, a liquid pump and a cold drain; the automatic PCR amplification mechanism comprises a PCR sample pool, a PCR hot cover and a motor set; the PCR sample pool, the refrigerating sheet and the liquid cooling plate are arranged in a clinging manner from top to bottom, and the liquid cooling plate is fixed on the base; the liquid cooling plate, the liquid pump and the cold drain are connected through pipes, liquid is contained in the liquid cooling plate, the liquid pump and the cold drain, and the liquid circulates among the liquid cooling plate, the liquid pump and the cold drain under the action of the water pump; the motor set is provided with a transmission structure and drives the PCR hot cover to realize translation and lifting movement through the transmission structure. The PCR sample pool can be efficiently cooled and effectively controlled in temperature by matching the liquid cooling heat dissipation mechanism; the automatic PCR amplification mechanism can effectively improve the working efficiency, and can be remotely controlled to improve the safety.
Description
Technical Field
The invention relates to PCR amplification equipment, in particular to liquid cooling automatic PCR equipment.
Background
The Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the biggest characteristic of the PCR is that trace amount of DNA can be greatly increased. Therefore, even if only a single spot of DNA can be isolated from the hairs, skin or blood left by the murder in the murder case, or the remains of the historic creatures and the historical characters in the fossil, the DNA can be amplified by PCR and compared.
The PCR (Polymerase Chain Reaction) instrument is a life science instrument with wide application and great importance. The gene amplification device is widely applied to various fields of life science research, biochemical analysis, clinical diagnosis, drug analysis, forensic identification, epidemic situation rapid inspection and the like as the first choice instrument equipment for gene amplification analysis.
PCR is the mass synthesis of In Vitro or In Vitro of a specific gene using DNA polymerase, and basically it is a specific linkage replication using DNA polymerase. The current technology can copy a segment of gene from one hundred billion to one billion times. PCR instruments can be classified into four types, i.e., general PCR instruments, gradient PCR instruments, in-situ PCR instruments and real-time fluorescence quantitative PCR instruments, according to the purpose of DNA amplification and the standard of detection. The basic PCR must have 1. the DNA template to be replicated, template2. primers defining the two ends of the replication range, primers, 3.DNA polymerase Taq. Polymearse4. synthetic material (four deoxynucleotides) and water.
The working process of the PCR instrument is as follows: 1. "denaturation": heating the sample to 94-96 ℃ takes 30 seconds to several minutes, and the DNA double strand is broken into two single strands 2, "annealed": allowing the temperature of the sample to drop to 50-65 ℃ for 30 seconds to minutes, the two single strands binding to the DNA primer previously added to the reagent; 3. "extend": allowing the temperature of the sample to rise to 72 ℃ takes from 30 seconds to minutes and the discrete bases in the reagent bind to the remainder of the two single strands into two separate double strands. The whole culture process is approximately performed through 30 cycles. Therefore, PCR amplification equipment can produce a large amount of heats in the course of the work in fact, and the heat dissipation condition of present PCR amplification equipment is crude, mostly only is equipped with simple air-cooled heat dissipation, and the radiating efficiency is low, is difficult to ensure rapid cooling, produces the influence to PCR amplification process easily.
In addition, certain harmfulness exists during partial PCR amplification, such as germ PCR amplification, and certain potential safety hazards exist in the operation process of operators, so that the realization of the remote automatic operation of PCR amplification is also a great development trend of the existing PCR amplification equipment.
For example, the PCR amplification reaction apparatus disclosed by the chinese patent office on 8/26/2009 and the invention patent application of the PCR amplification reaction method using the apparatus, which is published under the application publication number CN100532567C, are similar to most existing PCR amplification devices, and are provided with a heating element and a sample cell, and the sample cell is heated by the heating element and then simply cooled by air cooling to dissipate heat, and needs to be completely manually operated.
Disclosure of Invention
The invention provides liquid cooling automatic PCR equipment, aiming at solving the problems that the existing PCR equipment is too simple in structure and low in heat dissipation efficiency, poor heat dissipation is easy to cause amplification failure or poor effect in the PCR amplification process, operators need to operate fully manually during PCR amplification of part of pathogens, certain potential safety hazards exist, manual operation efficiency is low and the like. The invention aims to: firstly, high-efficiency and effective heat dissipation can be realized, and temperature control can be realized; automatic operation can be realized, and the operation safety and the working efficiency are improved; thirdly, the whole structure is compact, and the occupied space is reduced.
In order to achieve the purpose, the invention adopts the following technical scheme.
A liquid-cooling automatic PCR device, which comprises a PCR chamber,
the device comprises a base, a liquid cooling heat dissipation mechanism and an automatic PCR amplification mechanism, wherein the liquid cooling heat dissipation mechanism and the automatic PCR amplification mechanism are arranged on the base;
the liquid cooling heat dissipation mechanism comprises a refrigerating sheet for refrigerating, a liquid cooling plate for cooling the refrigerating sheet, a liquid pump for realizing liquid circulation and a cold drain for cooling circulating liquid;
the automatic PCR amplification mechanism comprises a detachable PCR sample pool, a detachable PCR hot cover and a motor set arranged on a base;
the PCR sample pool, the refrigerating sheet and the liquid cooling plate are arranged in a clinging manner from top to bottom, and the liquid cooling plate is fixed on the base;
the liquid cooling plate, the liquid pump and the cold drain are connected through pipes, liquid is contained in the liquid cooling plate, the liquid pump and the cold drain, and the liquid circulates among the liquid cooling plate, the liquid pump and the cold drain under the action of the water pump;
the motor set is provided with a transmission structure and drives the PCR hot cover to realize translation and lifting movement through the transmission structure.
As a preference, the first and second liquid crystal compositions are,
the motor set is provided with at least one translation motor and one lifting motor;
the transmission structure correspondingly arranged on the translation motor comprises a translation screw rod, a translation guide rail and a translation platform;
one end of the translation screw rod is connected with a translation motor and is driven by the translation motor to rotate, and the other end of the translation screw rod points to the PCR sample pool;
the translation guide rail is parallel to the translation screw rod, the translation platform is sleeved on the translation screw rod, a sink groove matched with the translation guide rail is formed in the lower end of the translation platform and is connected to the translation guide rail in a clamping mode through the sink groove, the translation guide rail limits the translation platform to rotate, and the translation screw rod can drive the translation platform to perform translation reciprocating motion;
the lifting motor is fixedly arranged at the upper end of the translation platform, and the correspondingly arranged transmission structure comprises a lifting screw rod, a lifting guide rail and a lifting platform;
one end of the lifting screw rod is connected with a lifting motor and driven by the lifting motor to rotate, and the other end of the lifting screw rod faces upwards;
the lifting guide rail and the lifting screw rod are arranged in parallel, and the lifting platform is clamped on the lifting guide rail or sleeved on the lifting screw rod and is clamped with the lifting guide rail for limiting;
a connecting arm is arranged on the side surface of the lifting platform, the inner end of the connecting arm is fixedly connected with the lifting platform, the outer end of the connecting arm is parallel to the translation guide rail and points to the upper part of the PCR sample pool, and the end part of the outer end of the connecting arm is fixedly connected with the upper end surface of the PCR heat cover;
when the lifting platform is clamped on the lifting guide rail, the connecting arm is sleeved on the lifting screw rod.
As a preference, the first and second liquid crystal compositions are,
the liquid cooling automatic PCR equipment is also provided with a photoelectric sensor for detecting the translation and the lifting of the PCR hot cover, the photoelectric sensor is connected with the electric signal of the motor set and controls the motor set to work through a feedback signal.
As a preference, the first and second liquid crystal compositions are,
the photoelectric sensor is divided into a translation photoelectric sensor for detecting the translation movement distance of the PCR thermal cover and a lifting photoelectric sensor for detecting the lifting movement distance of the PCR thermal cover;
the translation photoelectric sensor is arranged on the side surface of the translation platform;
the lifting photoelectric sensor is arranged on the side surface of the lifting platform.
As a preference, the first and second liquid crystal compositions are,
the refrigerating piece is a semiconductor refrigerating piece;
the upper end surface and the lower end surface of the refrigeration sheet are respectively a convertible cold end and a hot end.
As a preference, the first and second liquid crystal compositions are,
a liquid cavity is arranged in the liquid cooling plate;
a plurality of partition plates for connecting the upper wall and the lower wall of the liquid cavity are arranged in the liquid cavity;
the baffle plate separates the liquid cavity to form a liquid flow channel.
As a preference, the first and second liquid crystal compositions are,
the cold row comprises a fixed frame, a fan and a liquid cooling runner;
the fixing frame is fixedly arranged on the base, and the fan and the liquid cooling runner are fixed on the fixing frame;
the blowing direction of the fan faces the liquid cooling flow channel.
As a preference, the first and second liquid crystal compositions are,
the liquid cooling flow passages are arranged in a grid shape, a fence shape or a net shape.
The invention has the beneficial effects that:
1) the high-efficiency heat dissipation and the effective temperature control of the PCR sample pool can be realized through the matching of the liquid cooling heat dissipation mechanism;
2) the working efficiency can be effectively improved through the automatic PCR amplification mechanism, and the safety can be improved through remote control;
3) the whole structure is compact, and the occupied space is small;
4) the PCR sample pool and the PCR hot cover can be replaced, and the use flexibility is higher;
5) the operation is simple and easy to operate.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a schematic top view of the present invention;
FIG. 3 is a schematic axial view of a portion of the present invention;
FIG. 4 is an enlarged view of portion A of FIG. 3;
FIG. 5 is an enlarged view of portion B of FIG. 3;
FIG. 6 is a schematic isometric view of a portion of an automated PCR amplification mechanism of the present invention;
FIG. 7 is a schematic structural view of the cold row of the present invention;
FIG. 8 is a schematic view of the liquid-cooled panel of the present invention;
in the figure: the system comprises a base 1, a refrigerating sheet 2, a liquid cooling plate 3, a liquid cooling inlet interface 3-1, a liquid cooling outlet interface 3-2, a liquid cavity 3-3, a partition plate 3-4, a cold row 4, a fixing frame 4-1, a cold liquid inlet 4-2, a cold liquid outlet 4-3, a cold liquid flow passage 4-4, a fan 4-5, a liquid pump 5, a pump inlet 5-1, a pump outlet 5-2, a PCR sample pool 6, a translation motor 7, a translation screw rod 7-1, a translation guide rail 7-2, a translation platform 7-3, a lifting motor 8, a lifting screw rod 8-1, a lifting guide rail 8-2, a lifting platform 8-3, a connecting arm 9, a PCR hot cover 10-1, a heating element 10-1, a trapezoidal plate 11-1, a bolt 11-2, a clamping groove 12, a translation photoelectric sensor and a 13 photoelectric sensor.
Detailed Description
The invention is described in further detail below with reference to specific embodiments and the attached drawing figures. Those skilled in the art will be able to implement the invention based on these teachings. Moreover, the embodiments of the present invention described in the following description are generally only some embodiments of the present invention, and not all embodiments. Therefore, all other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present invention without any creative effort shall fall within the protection scope of the present invention.
Unless otherwise specified, technical means and conventional structures described in the embodiments of the present invention are known to those skilled in the art.
Examples
As shown in fig. 1 to 8, an automatic liquid-cooling PCR apparatus based on a liquid-cooling heat dissipation mechanism and an automation mechanism includes:
the base 1 to and liquid cooling heat dissipation mechanism, the automatic PCR amplification mechanism of setting on base 1.
The liquid cooling heat dissipation mechanism mainly comprises a refrigerating sheet 2, a liquid cooling plate 3, a cold row 4 and a liquid pump 5;
the refrigerating sheet 2 is a semiconductor refrigerating sheet 2, the upper end face and the lower end face of the refrigerating sheet 2 are respectively a convertible cold end and a hot end, a closed circuit formed by X and Y different metal wires is arranged in the refrigerating sheet 2, when the upper end face of the refrigerating sheet needs to absorb heat, the upper end face is cold-end heat absorption, the lower end face is hot-end heat release, when the upper end face does not need to absorb heat, the lower end face is cold-end heat absorption, the upper end face is hot-end heat release, the cold end and the hot end can be interchanged by controlling input current, namely reverse current is applied, and the operation is simple;
the liquid cooling plate 3 is fixedly arranged on the base 1, a liquid cavity 3-3 or a liquid flow channel is arranged in the liquid cooling plate, a liquid cooling liquid inlet interface 3-1 and a liquid cooling liquid outlet interface 3-2 are correspondingly arranged in the liquid cooling plate, and the liquid cavity 3-3 and the liquid flow channel as well as the liquid cooling liquid inlet interface 3-1 and the liquid cooling liquid outlet interface 3-2 are used for liquid circulation;
the liquid cavity 3-3 in the liquid cooling plate 3 is provided with a plurality of partition plates 3-4 for connecting the upper wall and the lower wall, the partition plates 3-4 partition the liquid cavity 3-3 in the liquid cooling plate 3 to further form a liquid flow channel, and the partition plates 3-4 and the main body of the liquid cooling plate 3 are made of high heat conduction materials, so that the liquid cooling plate 3 can be more quickly conducted into the liquid cavity 3-3 after absorbing the heat of the refrigerating sheet 2, the heat dissipation area of the liquid cooling plate 3 is increased when contacting with liquid for heat dissipation, and the heat dissipation efficiency is improved;
the refrigerating pieces 2 and the liquid cooling plate 3 are arranged in a clinging manner, at least one refrigerating piece 2 is arranged, as shown in fig. 3, three refrigerating pieces 2 are arranged on the upper end face of the liquid cooling plate 3, the three refrigerating pieces 2 are uniformly arranged in one direction, and the lower end face of each refrigerating piece 2 is in close contact with the upper end face of the liquid cooling plate 3;
a liquid cooling liquid inlet interface 3-1 of the liquid cooling plate 3 is connected to a pump outlet 5-2 of the liquid pump 5, and the liquid cooling plate can be connected through any pipe fitting, and is optimally a hose;
a liquid cooling liquid outlet interface 3-2 of the liquid cooling plate 3 is connected to a cold liquid inlet 4-2 of the cold bar 4, and the liquid cooling plate can be connected through any pipe fitting, and is optimally a hose;
the cold row 4 is also provided with a cold liquid outlet 4-3 besides the cold liquid inlet 4-2, the cold row 4 is also provided with a fixed frame 4-1, a cold liquid flow passage 4-4 and a fan 4-5, the bottom end of the fixed frame 4-1 is fixed on the base 1, two ends of the cold liquid flow passage 4-4 are respectively connected with the cold liquid outlet 4-3 and the cold liquid inlet 4-2, and the cold liquid flow passage 4-4 is optimally arranged in a grid shape or a fence shape or a net shape as shown in fig. 7, the arrangement mode can greatly increase the heat dissipation area of the cold liquid flow passage 4-4, so that the liquid flowing through the cold liquid flow passage 4-4 can be more fully dissipated;
the fan 4-5 and the liquid cooling flow passage are fixedly arranged on the fixed frame 4-1, and the blowing direction of the fan faces the cold liquid flow passage 4-4, so that the heat dissipation speed of the liquid in the cold liquid flow passage 4-4 is increased, and the liquid is cooled rapidly;
the cold liquid outlet 4-3 of the cold row 4 is connected to a pump inlet 5-1 of a liquid pump 5, which may be connected by any pipe, preferably a hose.
When the heat absorption/heat dissipation function is realized by the integral liquid cooling heat dissipation mechanism:
firstly, enough liquid is ensured to be contained in the whole liquid-cooling heat dissipation mechanism, the liquid can be liquid or liquid capable of absorbing heat in a conventional way such as methanol or ethanol and the like, or heat conducting liquid obtained by special procurement, then a liquid pump 5 is opened to start working at a working swab, so that the liquid starts to pre-flow for 5-6 s and then starts to work, the upper end surface of a refrigerating sheet 2 absorbs heat at a cold end during working, the heat after absorbing heat is transferred to the hot end of the lower end surface of the refrigerating sheet 2 to release heat, the heat released at the hot end of the lower end surface of the refrigerating sheet 2 is transferred to a liquid cooling plate 3 and absorbed by the liquid cooling plate 3, the heat absorbed by the liquid cooling plate 3 is taken away by the liquid flowing through the liquid cooling plate 3 to a cold row 4, the liquid flowing through the liquid cooling plate 3 is heated and then flows into a cold liquid flow passage 4-4 to perform sufficient heat dissipation, particularly, the high-efficiency and sufficient heat dissipation are realized under the action of a fan 4-5 of the cold row 4, and the, finally, the liquid pump 5 pumps the liquid to the liquid cooling plate 3, and then the steps of heat absorption and heat dissipation of the liquid are continuously repeated, so that liquid circulation of the liquid cooling heat dissipation mechanism is realized.
The automatic PCR amplification mechanism mainly comprises a motor set, a PCR hot cover 10 and a PCR sample pool 6, wherein the PCR hot cover 10 and the PCR sample pool 6 are replaceable;
the PCR sample pool 6 is arranged at the upper end of the refrigerating sheet 2 and is tightly contacted with the refrigerating sheet 2;
the motor set consists of at least two motors, as shown in fig. 6, which consists of a translation motor 7 and a lifting motor 8;
the translation motor 7 is connected with a translation auxiliary mechanism, the translation auxiliary mechanism comprises a translation screw rod 7-1 arranged parallel to the upper end face of the base 1, one end of the translation screw rod 7-1 is connected with the translation motor 7, and the other end of the translation screw rod points to the PCR sample pool 6;
the translation screw 7-1 is further sleeved with a translation platform 7-3, at least one translation guide rail 7-2 arranged in parallel with the translation screw 7-1 is further arranged below the translation screw 7-1, a trap groove is formed in the lower end of the translation platform 7-3 and matched with the translation guide rail 7-2, the trap groove and the translation guide rail 7-2 are matched with each other to prevent the translation platform 7-3 from rotating, the translation motor 7 drives the translation screw 7-1 to rotate after being started, and the trap groove and the translation guide rail 7-2 are matched to limit the translation platform 7-3 to rotate, so that the translation platform 7-3 can realize fixed-distance reciprocating translation movement along the translation screw 7-1;
a lifting motor 8 is fixedly arranged on the translation platform 7-3, and a lifting auxiliary mechanism is also arranged at the upper end of the lifting motor 8 in a matched manner;
the lifting auxiliary mechanism can be a conventional lifting mechanism, and can also be provided with a lifting screw rod 8-1 and a lifting guide rail 8-2 like a translation auxiliary mechanism, preferably provided with a lifting screw rod 8-1 and a lifting guide rail 8-2, and has higher stability and controllability compared with the conventional lifting mechanism, the lifting screw rod 8-1 is vertical to the base 1, the lower end of the lifting screw rod is connected with the lifting motor 8, the lifting guide rail 8-2 is parallel to the lifting screw rod 8-1, a lifting platform 8-3 capable of realizing fixed-distance lifting reciprocating motion under the driving action of the lifting motor 8 is arranged by matching with the conventional lifting mechanism or the lifting screw rod 8-1 and the lifting guide rail 8-2, and the PCR heat cover 10 is fixedly arranged relative to the lifting platform 8-3;
as shown in FIG. 6, the upper end of the PCR hot lid 10 is fixedly connected with a connecting arm 9, and the connecting arm 9 is fixedly connected with the lifting platform 8-3;
the lower end surface of the PCR hot cover 10 is provided with a heating element 10-1, the heating element 10-1 is a heating film or a heating plate or a heating sheet or an electric heating wire, the heating element is connected with an external power supply through a circuit or a motor is arranged on the rest part of the liquid cooling automatic PCR equipment to drive the liquid cooling automatic PCR equipment to work, and the external power supply is preferably connected with the heating element, so that the volume of the liquid cooling automatic PCR equipment can be effectively reduced, and the load during the whole work is reduced;
in order to improve the stability of the lifting motion of the connecting arm 9, the lifting platform 8-3 is sleeved or clamped on the lifting guide rail 8-2, and the connecting arm 9 is sleeved on the lifting screw rod 8-1 and is fixedly connected with the lifting platform 8-3;
when the PCR hot cover 10 is used for tightly covering the PCR sample pool 6:
firstly, a thermal motor is started to preheat a PCR hot cover 10, then a translation motor 7 works to drive a translation screw rod 7-1 to rotate, further drive a translation platform 7-3 to perform translation motion, the translation platform 7-3 moves to a proper distance towards the direction of a PCR sample pool 6, the translation motor 7 stops working after the PCR hot cover 10 is just positioned right above the PCR sample pool 6, the stability of the translation platform 7-3 is kept, then a lifting motor 8 works to drive the lifting platform 8-3 to press down through a lifting auxiliary mechanism, further drive a connecting arm 9 and the PCR hot cover 10 fixedly connected with the connecting arm 9 to press down until the PCR hot cover 10 presses the PCR sample pool 6 tightly, and the covering work is realized, otherwise, the PCR hot cover 10 is lifted to reset, and the translation platform 7-3 moves towards the translation motor 7 to reset;
by the working principle, a tester can be far away from a PCR amplification instrument for remote operation during PCR amplification, and can set the timing and constant-power starting, closing or reversing of the translation motor 7 and the lifting motor 8 to realize the fixed-distance movement of the translation platform 7-3 and the lifting platform 8-3, so as to realize automatic operation;
in order to further improve the precision of aligning the PCR hot cover 10 with the PCR sample pool 6 and the universality of the PCR sample pools 6 and the PCR hot covers 10 with different specifications, the automatic PCR amplification mechanism is also provided with a photoelectric sensor;
the photoelectric sensor comprises but not limited to an infrared distance sensor, is mainly used for detecting the translation and lifting distance of the PCR thermal cover 10, realizes the accurate detection of the translation distance of the translation platform 7-3 by arranging the translation photoelectric sensor 12 on the translation platform 7-3 and arranging the corresponding conventional arrangement mode of the translation photoelectric sensor 12 along the translation direction, feeds back an electric signal to the translation motor 7 through the translation photoelectric sensor 12 so as to further realize the accurate control of the translation distance of the translation platform 7-3, then arranges the lifting photoelectric sensor 13 on the lower end surface of the lifting platform 8-3 and correspondingly arranges the built-in or external lifting photoelectric sensor 13 on the upper end surface of the lifting motor 8 so as to realize the accurate detection of the lifting distance of the lifting platform 8-3, feeds back the electric signal to the lifting motor 8 through the lifting photoelectric sensor 13, thereby realizing the accurate control of the lifting distance of the lifting platform 8-3;
the arrangement of the translation photoelectric sensor 12 and the lifting photoelectric sensor 13 can further improve the automation degree of the automatic PCR amplification mechanism and the accuracy of the PCR sample pool 6 covered tightly by the PCR hot cover 10, improve the working stability of the whole automatic PCR amplification mechanism and basically avoid the generation of working errors completely.
the opposite surfaces of the oppositely arranged trapezoidal plates 11 are provided with bolts 11-1 to form clamping grooves 11-2, the edges of the PCR sample pool 6 can be clamped in the clamping grooves 11-2, and the placing stability of the PCR sample pool 6 is further improved.
When the whole works:
firstly, selecting a needed PCR sample pool 6 and a PCR hot cover 10 which is matched with the needed PCR sample pool 6, fixedly connecting the PCR hot cover 10 with a connecting arm 9 and electrically connecting the PCR hot cover 10 with a thermal motor, arranging the PCR sample pool 6 at the upper end of a refrigerating sheet 2 to be tightly contacted with the refrigerating sheet 2, clamping the edge of the PCR sample pool 6 in a clamping groove 11-2 of a trapezoidal plate 11, after the manual preparation work is finished, setting working parameters of a translation motor 7, a lifting motor 8, the thermal motor and a liquid pump 5 according to the specification, the size and the experimental requirements of the selected PCR sample pool 6 and the PCR hot cover 10, if the thermal cover moves forward 10cm and moves downward 5cm, covering the PCR sample pool 6 tightly, and if the experimental requirements of pre-denaturation heating for 180s, constant temperature amplification for 40s, cooling annealing for 30s and heating for 60s are carried out for 30 cycles and then constant temperature for 420s, setting the parameters of the translation motor 7 to drive a translation platform 7-3 to move towards, Set up the hot lid 10 of elevator motor 8 parameter for can driving PCR and move 5cm downwards, carry out translation and lift distance's real-time supervision through photoelectric sensor at this in-process, ensure that the hot lid 10 of PCR can accurately carry out translation and lift work to, set up the work of thermoelectric motor and liquid pump 5, ensure that its intensification, constant temperature and cooling step can go on according to the regulation.
According to the working mode, after the manual preparation work is completed and the parameter setting is completed, the full-automatic work can be realized, so that the labor cost can be reduced, and the PCR sample pollution caused by manual misoperation and the accidental occurrence of injury or infection of experimenters caused by manual misoperation can be avoided.
Claims (8)
1. A liquid cooling automatic PCR device is characterized in that,
the device comprises a base, a liquid cooling heat dissipation mechanism and an automatic PCR amplification mechanism, wherein the liquid cooling heat dissipation mechanism and the automatic PCR amplification mechanism are arranged on the base;
the liquid cooling heat dissipation mechanism comprises a refrigerating sheet for refrigerating, a liquid cooling plate for cooling the refrigerating sheet, a liquid pump for realizing liquid circulation and a cold drain for cooling circulating liquid;
the automatic PCR amplification mechanism comprises a detachable PCR sample pool, a detachable PCR hot cover and a motor set arranged on a base;
the PCR sample pool, the refrigerating sheet and the liquid cooling plate are arranged in a clinging manner from top to bottom, and the liquid cooling plate is fixed on the base;
the liquid cooling plate, the liquid pump and the cold drain are connected through pipes, liquid is contained in the liquid cooling plate, the liquid pump and the cold drain, and the liquid circulates among the liquid cooling plate, the liquid pump and the cold drain under the action of the water pump;
the motor set is provided with a transmission structure and drives the PCR hot cover to realize translation and lifting movement through the transmission structure.
2. The liquid-cooled automated PCR device according to claim 1,
the motor set is provided with at least one translation motor and one lifting motor;
the transmission structure correspondingly arranged on the translation motor comprises a translation screw rod, a translation guide rail and a translation platform;
one end of the translation screw rod is connected with a translation motor and is driven by the translation motor to rotate, and the other end of the translation screw rod points to the PCR sample pool;
the translation guide rail is parallel to the translation screw rod, the translation platform is sleeved on the translation screw rod, a sink groove matched with the translation guide rail is formed in the lower end of the translation platform and is connected to the translation guide rail in a clamping mode through the sink groove, the translation guide rail limits the translation platform to rotate, and the translation screw rod can drive the translation platform to perform translation reciprocating motion;
the lifting motor is fixedly arranged at the upper end of the translation platform, and the correspondingly arranged transmission structure comprises a lifting screw rod, a lifting guide rail and a lifting platform;
one end of the lifting screw rod is connected with a lifting motor and driven by the lifting motor to rotate, and the other end of the lifting screw rod faces upwards;
the lifting guide rail and the lifting screw rod are arranged in parallel, and the lifting platform is clamped on the lifting guide rail or sleeved on the lifting screw rod and is clamped with the lifting guide rail for limiting;
a connecting arm is arranged on the side surface of the lifting platform, the inner end of the connecting arm is fixedly connected with the lifting platform, the outer end of the connecting arm is parallel to the translation guide rail and points to the upper part of the PCR sample pool, and the end part of the outer end of the connecting arm is fixedly connected with the upper end surface of the PCR heat cover;
when the lifting platform is clamped on the lifting guide rail, the connecting arm is sleeved on the lifting screw rod.
3. The liquid-cooled automated PCR apparatus according to claim 1 or 2,
the liquid cooling automatic PCR equipment is also provided with a photoelectric sensor for detecting the translation and the lifting of the PCR hot cover, the photoelectric sensor is connected with the electric signal of the motor set and controls the motor set to work through a feedback signal.
4. The liquid-cooled automated PCR device according to claim 3,
the photoelectric sensor is divided into a translation photoelectric sensor for detecting the translation movement distance of the PCR thermal cover and a lifting photoelectric sensor for detecting the lifting movement distance of the PCR thermal cover;
the translation photoelectric sensor is arranged on the side surface of the translation platform;
the lifting photoelectric sensor is arranged on the side surface of the lifting platform.
5. The liquid-cooled automated PCR device according to claim 1,
the refrigerating piece is a semiconductor refrigerating piece;
the upper end surface and the lower end surface of the refrigeration sheet are respectively a convertible cold end and a hot end.
6. The liquid-cooled automated PCR device according to claim 1,
a liquid cavity is arranged in the liquid cooling plate;
a plurality of partition plates for connecting the upper wall and the lower wall of the liquid cavity are arranged in the liquid cavity;
the baffle plate separates the liquid cavity to form a liquid flow channel.
7. The liquid-cooled automated PCR device according to claim 1,
the cold row comprises a fixed frame, a fan and a liquid cooling runner;
the fixing frame is fixedly arranged on the base, and the fan and the liquid cooling runner are fixed on the fixing frame;
the blowing direction of the fan faces the liquid cooling flow channel.
8. The liquid-cooled automated PCR device according to claim 7,
the liquid cooling flow passages are arranged in a grid shape, a fence shape or a net shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910924351.4A CN111229350A (en) | 2019-09-27 | 2019-09-27 | Liquid cooling automatic PCR equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910924351.4A CN111229350A (en) | 2019-09-27 | 2019-09-27 | Liquid cooling automatic PCR equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111229350A true CN111229350A (en) | 2020-06-05 |
Family
ID=70871030
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910924351.4A Pending CN111229350A (en) | 2019-09-27 | 2019-09-27 | Liquid cooling automatic PCR equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111229350A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112063491A (en) * | 2020-09-15 | 2020-12-11 | 厦门大学 | Micro-channel temperature control device and method for nucleic acid detection amplification reaction |
| CN112638124A (en) * | 2020-12-16 | 2021-04-09 | 烟台职业学院 | Integrated circuit heat sink |
| CN114854569A (en) * | 2022-06-17 | 2022-08-05 | 南京溯远基因科技有限公司 | Thermal cycling device and PCR instrument |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2351779Y (en) * | 1998-09-29 | 1999-12-01 | 杭州大和热磁电子有限公司 | Gene magnification instrument |
| JP2005006507A (en) * | 2003-06-16 | 2005-01-13 | Aisin Cosmos R & D Co Ltd | Incubator |
| US20090275034A1 (en) * | 2008-03-05 | 2009-11-05 | Helicos Biosciences Corporation | Temperature control system |
| CN103333789A (en) * | 2013-06-21 | 2013-10-02 | 中国科学院上海技术物理研究所 | Convenient-to-integrate device for achieving PCR (polymerase chain reaction) and operating method |
| CN203976778U (en) * | 2014-06-16 | 2014-12-03 | 杭州安杰思生物科技有限公司 | The hot lid mechanism of a kind of automatic shutter for gene-amplificative instrament |
| CN204022809U (en) * | 2014-05-19 | 2014-12-17 | 苏州东胜兴业科学仪器有限公司 | The gene amplification device with liquid-cooling system |
| CN104293645A (en) * | 2014-08-27 | 2015-01-21 | 南京普东兴生物科技有限公司 | Liquid cooling heat-radiation apparatus used for sequencing device |
| CN104630057A (en) * | 2015-03-05 | 2015-05-20 | 东南大学 | Full-automatic thermal cycler hot cover |
| CN207095081U (en) * | 2017-08-01 | 2018-03-13 | 四川新健康成生物股份有限公司 | A kind of cold semiconductor cooling device of liquid for Medical Devices |
| CN207435446U (en) * | 2017-09-29 | 2018-06-01 | 重庆京因生物科技有限责任公司 | A kind of PCR instrument temperature-controlling system |
| CN209178400U (en) * | 2018-11-07 | 2019-07-30 | 莫纳（苏州）生物科技有限公司 | A kind of PCR instrument with sealer function |
-
2019
- 2019-09-27 CN CN201910924351.4A patent/CN111229350A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2351779Y (en) * | 1998-09-29 | 1999-12-01 | 杭州大和热磁电子有限公司 | Gene magnification instrument |
| JP2005006507A (en) * | 2003-06-16 | 2005-01-13 | Aisin Cosmos R & D Co Ltd | Incubator |
| US20090275034A1 (en) * | 2008-03-05 | 2009-11-05 | Helicos Biosciences Corporation | Temperature control system |
| CN103333789A (en) * | 2013-06-21 | 2013-10-02 | 中国科学院上海技术物理研究所 | Convenient-to-integrate device for achieving PCR (polymerase chain reaction) and operating method |
| CN204022809U (en) * | 2014-05-19 | 2014-12-17 | 苏州东胜兴业科学仪器有限公司 | The gene amplification device with liquid-cooling system |
| CN203976778U (en) * | 2014-06-16 | 2014-12-03 | 杭州安杰思生物科技有限公司 | The hot lid mechanism of a kind of automatic shutter for gene-amplificative instrament |
| CN104293645A (en) * | 2014-08-27 | 2015-01-21 | 南京普东兴生物科技有限公司 | Liquid cooling heat-radiation apparatus used for sequencing device |
| CN104630057A (en) * | 2015-03-05 | 2015-05-20 | 东南大学 | Full-automatic thermal cycler hot cover |
| CN207095081U (en) * | 2017-08-01 | 2018-03-13 | 四川新健康成生物股份有限公司 | A kind of cold semiconductor cooling device of liquid for Medical Devices |
| CN207435446U (en) * | 2017-09-29 | 2018-06-01 | 重庆京因生物科技有限责任公司 | A kind of PCR instrument temperature-controlling system |
| CN209178400U (en) * | 2018-11-07 | 2019-07-30 | 莫纳（苏州）生物科技有限公司 | A kind of PCR instrument with sealer function |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112063491A (en) * | 2020-09-15 | 2020-12-11 | 厦门大学 | Micro-channel temperature control device and method for nucleic acid detection amplification reaction |
| CN112638124A (en) * | 2020-12-16 | 2021-04-09 | 烟台职业学院 | Integrated circuit heat sink |
| CN114854569A (en) * | 2022-06-17 | 2022-08-05 | 南京溯远基因科技有限公司 | Thermal cycling device and PCR instrument |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111229350A (en) | Liquid cooling automatic PCR equipment | |
| CN105219638B (en) | A kind of PCR instrument device based on phase change heat storage material and heat pipe | |
| CN110724631B (en) | Heating control device of nucleic acid amplification instrument | |
| JP2022527876A (en) | Reactor and reaction system for detection chips | |
| CN112063491B (en) | Micro-channel temperature control device and method for nucleic acid detection amplification reaction | |
| KR20220041207A (en) | Ultrafast PCR reaction detection system and detection method | |
| KR102288369B1 (en) | Integrated chip with lysis and amplifying nucleic acids, lysis module having the same, apparatus for point-of-care-testing having the same, and method for lysis and amplifying nucleic acids using the same | |
| CN111004708A (en) | PCR temperature cycle control method and rotary drive type PCR temperature cycle control device | |
| CN206244801U (en) | PCR gene amplification instrument module mechanism | |
| CN103333789A (en) | Convenient-to-integrate device for achieving PCR (polymerase chain reaction) and operating method | |
| CN111203293A (en) | Novel intelligent quarantine inspection bench and quarantine method | |
| CN107389391B (en) | Automatic dewaxing antigen repair system | |
| CN210923478U (en) | Real-time fluorescent quantitative PCR instrument for on-site rapid detection | |
| US6815198B2 (en) | Apparatus for automated preparation of DNA samples and reactor for preparing DNA samples | |
| CN106047685A (en) | Fluorescent quantitative PCR (polymerase chain reaction) instrument | |
| CN213475992U (en) | PCR thermal cycling device | |
| CN210090145U (en) | Dyeing machine for LCT liquid-based thin-layer cells and cell DNA | |
| CN212932128U (en) | Full-automatic pathological section dyeing system | |
| CN213203140U (en) | Isothermal normalizing device for gear | |
| CN210945695U (en) | Heat treatment device for metal material | |
| CN210314197U (en) | PCR amplification detection device | |
| CN216764929U (en) | Nucleic acid extraction amplification detection kit | |
| CN208256706U (en) | A kind of light decay equipment | |
| CN109269873A (en) | Magneto separate heating device for the processing of high-throughput biological sample | |
| CN214538902U (en) | Appearance is cleared up to COD intelligence |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200605 |