CN112063491B - Micro-channel temperature control device and method for nucleic acid detection amplification reaction - Google Patents
Micro-channel temperature control device and method for nucleic acid detection amplification reaction Download PDFInfo
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Abstract
A micro-channel temperature control device and a method for nucleic acid detection and amplification reaction relate to a micro-channel temperature control device and a method. The device comprises a nucleic acid amplification reaction module, a temperature rising/reducing module, a micro-channel cooling module and a microcontroller module; the nucleic acid amplification reaction module and the micro-channel cooling module are respectively arranged on the upper surface and the lower surface of the temperature rising/reducing module; the nucleic acid amplification reaction module comprises a reaction plate, a reaction column and a reaction tube; the temperature rising/lowering module is attached to the bottom of the reaction plate, and the temperature rising/lowering and temperature control of the reaction module are realized through the microcontroller module; the microchannel cooling module comprises a microchannel plate, a microchannel cover plate, a liquid pipe, a cooling fan, a condenser, a liquid reservoir and a water pump; thermocouples are arranged at the outlets of the reaction column, the thermoelectric refrigerating sheet and the condenser and used for acquiring temperature signals. The outer layer heat insulation material is not used, the temperature distribution uniformity is improved, the cost is reduced, and the production operation convenience is improved. The power of the thermoelectric refrigerating sheet is improved, and the temperature rising/reducing rate of the reaction module is improved.
Description
Technical Field
The invention relates to a micro-channel temperature control device and a micro-channel temperature control method, in particular to a micro-channel temperature control device and a micro-channel temperature control method for nucleic acid detection amplification reaction.
Background
In recent years, with the development of gene technology, nucleic acid detection has played an increasingly important role. In clinic, through nucleic acid detection, pathological changes can be found in advance and treated as soon as possible, so that the cure rate is improved. Currently, infectious diseases are popular, various viruses are abused, and the physical health of people is seriously affected. New coronary pneumonia is prevalent worldwide and has caused millions of infections. The latent period of the new coronary pneumonia is long, and asymptomatic infection exists, which brings great challenges to the prevention and treatment of epidemic situations. The most important epidemic prevention means at present is large-scale nucleic acid detection, early discovery, early isolation and early treatment are achieved, and good epidemic prevention effects are achieved in China.
Because the concentration of the collected nucleic acid detection sample is low, the existing equipment is difficult to detect, and a special mode needs to be adopted to improve the concentration of the nucleic acid. At present, the commonly used mode is Polymerase Chain Reaction (PCR Reaction for short), DNA can be denatured and dissociated into single strands under certain conditions, then the DNA and heterologous nucleic acid form double strands, firstly, template gene is thermally denatured, the complementary sequences of the primer and the single strand DNA are renatured by cooling to form a template and primer compound (annealing), then, the primer is taken as a fixed starting point, heat-resistant DNA Polymerase is used for synthesizing DNA (primer extension) from 5 'to 3' direction, namely, denaturation-annealing-extension is a cycle, and after 20-30 cycles, the nucleic acid is amplified by 2 timesnAnd (4) doubling, and performing nucleic acid detection. The polymerase chain reaction has high sensitivity, strong specificity, simplicity, convenience and rapidness, and is an effective nucleic acid amplification means.
Because the nucleic acid amplification reaction needs to carry out multiple temperature rise/reduction operations on the sample, and strict requirements are put on the temperature rise/reduction rate, the temperature rise and reduction rate of the sample is generally required to be not lower than 5 ℃/s. At present, the nucleic acid detection equipment on the market adopts a heat dissipation mode of thermoelectric refrigeration sheets and fins, and because the heat dissipation efficiency of the fins is low, only thermoelectric refrigeration sheets with lower power can be adopted, so that the temperature rise/fall rate of a sample in a reaction module is not enough. Especially in the cooling process, the hot end produces a large amount of heats during thermoelectric refrigeration piece work, and the fin can't in time derive the heat, leads to the heat to scurry to the cold junction, has further reduced refrigeration effect, and cooling rate is far away can't satisfy current detection requirement. On the other hand, the reaction column and the reaction tube at the periphery of the reaction module exchange heat with the ambient air, so that the temperature of each sample is inconsistent, the concentration of the amplified nucleic acid of the sample is inconsistent, and the detection sensitivity and accuracy are seriously influenced. Therefore, the high-efficiency temperature control method for the nucleic acid detection amplification reaction is provided, and has important practical application significance for improving the accuracy and sensitivity of nucleic acid detection and shortening the detection time.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a micro-channel temperature control device and a micro-channel temperature control method for nucleic acid detection and amplification reaction. The device takes thermoelectric refrigeration sheets as temperature rising/lowering modules, the reaction plate is designed into a multilayer structure which is nested inside and outside, and the heat conductivity is gradually increased from the outer layer to the inner layer; the reaction column adopts materials with different heat conductivities, wherein the outer layer adopts a material with low heat conductivity, and the inner layer adopts a material with high heat conductivity.
Another objective of the invention is to provide a micro-channel temperature control method for nucleic acid detection and amplification reaction. The method can prevent temperature reduction caused by heat exchange between the outer side and the air, and improve temperature uniformity of the reaction module. The microchannel is used as a heat sink, and the heat of the reaction module is stored or released by virtue of the thermoelectric refrigerating sheet, so that the reaction module can be quickly heated and cooled, and the energy consumption is reduced. The heat generated by the operation of the thermoelectric refrigerating sheet is dissipated into the air through the cooling fan, so that the heat balance of the whole system is maintained.
The micro-channel temperature control device for the nucleic acid detection and amplification reaction comprises a nucleic acid amplification reaction module, a temperature rising/lowering module, a micro-channel cooling module and a microcontroller module; the nucleic acid amplification reaction module and the micro-channel cooling module are respectively arranged on the upper surface and the lower surface of the temperature rising/reducing module;
the nucleic acid amplification reaction module comprises a reaction plate, a reaction column and a reaction tube, wherein the reaction column is arranged in a hole of the reaction plate and used for fixing the reaction column, and the reaction tube is sleeved in the reaction column;
the temperature rising/lowering module is a thermoelectric refrigerating sheet and is attached to the bottom of the reaction plate, and the temperature rising/lowering and temperature control of the reaction module can be realized through the microcontroller module;
the microchannel cooling module comprises a microchannel plate, a microchannel cover plate, a liquid pipe, a cooling fan, a condenser, a liquid reservoir and a water pump; the microchannel plate is provided with a microchannel, and an inlet and an outlet of the microchannel are respectively connected with the liquid pipe; the microchannel cover plate is arranged at the upper part of the microchannel plate, and the top of the microchannel cover plate is attached to the temperature rising/reducing module; one end of the liquid pipe is respectively connected with an inlet and an outlet of the microchannel, the other end of the liquid pipe is respectively connected to a liquid storage device through a water pump and a condenser, a water cooling device is stored in the liquid storage device, and a liquid level controller and a liquid level sensor are arranged in the liquid storage device, wherein the liquid level controller can regulate and control cooling water participating in circulation according to a thermal balance condition; the water pump is used for driving cooling water to circulate between the micro-channel and the condenser, and the cooling water flowing in the liquid pipe takes away heat; thermocouples are arranged at the outlets of the reaction column, the thermoelectric refrigerating sheet and the condenser and are used for collecting temperature signals, the water pump is provided with a voltage signal sensor, and the liquid storage device is provided with a liquid level signal sensor;
and the microcontroller module is used for receiving the sensor signal and sending an instruction to control the temperature rise/fall of the thermoelectric refrigerating sheet and the participation amount and circulation rate of the cooling water.
The surplus heat released by the thermoelectric refrigerating sheet is dissipated into the air by the cooling fan; cooling water is stored in the liquid storage device, and a liquid level controller is arranged in the liquid storage device, so that the cooling water participating in circulation can be regulated and controlled according to the heat balance condition;
the reaction plate is of a multilayer structure nested inside and outside, and the heat conductivity of the reaction plate is gradually increased from the outer layer to the inner layer.
The inner and outer nested multilayer structure can adopt a first thermal conductivity material which is stainless steel, a second thermal conductivity material which is brass, a third thermal conductivity material which is aluminum alloy and a fourth thermal conductivity material which is red copper.
The reaction plate can adopt a 24-hole, 48-hole or 96-hole reaction plate.
The temperature rising/reducing module can adopt a thermoelectric refrigerating sheet.
The microchannel plate can be composed of a plurality of groups of snakelike microchannels which are not communicated with each other. The multiple groups can be provided with 5-10 groups.
The temperature control method of the nucleic acid detection amplification reaction microchannel comprises the following steps:
1) when the PCR reaction starts, the microcontroller gives a forward voltage to the thermoelectric refrigerating sheet, and at the moment, one surface of the thermoelectric refrigerating sheet, which is in contact with the reaction plate, is a hot end, and the other surface of the thermoelectric refrigerating sheet, which is in contact with the microchannel, is a cold end. Under the action of the Peltier effect, the heat of cooling water in the micro-channel is continuously released to the reaction module, meanwhile, the heat generated by the thermoelectric refrigerating sheet in the working process is also transmitted to the reaction module, and the temperature of the reaction module is rapidly increased to the rated upper temperature limit of 96 ℃; the micro-control module adjusts the current of the thermoelectric refrigerating sheet, so that the temperature of the reaction module is maintained at a rated temperature, and a high-temperature heat preservation link is carried out;
2) after the heat preservation is finished, the microcontroller module supplies a reverse voltage to the thermoelectric refrigerating sheet, the cold end and the hot end of the thermoelectric refrigerating sheet are switched, the heat of the reaction module is reversely transmitted to the microchannel, and cooling water in the microchannel is used as heat sink to store the heat;
in the step 2), the flow rate of the cooling water can be adjusted by a water pump according to the heat quantity released by the thermoelectric refrigerating sheet.
3) When the temperature of the reaction module is reduced to 30 ℃ below the rated lower temperature limit, the microcontroller module adjusts the current of the thermoelectric refrigerating sheet and enters a low-temperature heat preservation link.
4) After the low-temperature heat preservation link is finished, finishing one temperature rising/reducing cycle of the PCR reaction, namely entering the next cycle, and amplifying the number of nucleic acid in the reaction tube by one time;
5) the cooling fan is started to radiate redundant heat into the air, the heat balance of the system is maintained, the temperature at the beginning of the next temperature rising/reducing cycle is consistent with that of the previous cycle, and the temperature is detected by a thermocouple arranged at the outlet of the condenser and is transmitted to the microcontroller module.
The working principle of the invention is given below:
for the temperature uniformity, the reaction plate and the periphery of the reaction column are made of materials with low thermal conductivity, namely a layer of heat-insulating layer is added on the periphery of the reaction module, so that the heat exchange with the peripheral air is avoided, and the temperature uniformity of the reaction module can be improved. For the temperature rising/reducing rate, the micro-channel is used as a heat sink, is attached to the thermoelectric refrigerating sheet and is used for absorbing heat from the thermoelectric refrigerating sheet or releasing heat to the thermoelectric refrigerating sheet, and the heat is stored in cooling water in the micro-channel when the reaction module is cooled by virtue of the heat transfer function of the thermoelectric refrigerating sheet; when the temperature of the reaction module rises, heat is released from the microchannel. By the method, the reaction module can be rapidly heated and cooled. The water pump drives cooling water to circulate between the micro-channel and the condenser, and the surplus heat generated by the thermoelectric refrigerating sheet during working is transmitted to the condenser through the cooling water and is dissipated to the air through the cooling fan to maintain heat balance. And a liquid level controller in the liquid storage device can control the amount of cooling water participating in circulation according to the heat load of the reaction module. The reaction column and the thermoelectric refrigeration piece are provided with thermocouples for acquiring temperature signals, the water pump is provided with a voltage signal sensor, and the liquid storage device is provided with a liquid level signal sensor. The signals are transmitted to the microcontroller module, and the microcontroller module sends out instructions to control the temperature rise/fall of the thermoelectric refrigerating chip and the participation amount and circulation rate of the cooling water. The rapid temperature rise/fall and the uniformity of temperature distribution of nucleic acid amplification reaction can be realized by the heat regulation of the micro-channel heat sink and the design of the variable heat conductivity of the reaction plate.
Compared with the prior art, the micro-channel temperature control device and the method have the following advantages: the reaction plate and the reaction column are designed to have variable heat conductivity, so that an outer layer heat-insulating material is not used, the temperature distribution uniformity is improved, the cost is reduced, and the production and operation convenience is improved. The microchannel is used as the heat sink, and the microchannel has strong heat exchange performance and high heat storage and release speed, so that the power of the thermoelectric refrigerating sheet can be improved, and the temperature rising/reducing rate of the reaction module is further improved. In addition, because the specific heat capacity of water is large, a small amount of cooling water can store a large amount of heat, and the volume of equipment is reduced.
Drawings
FIG. 1 is a schematic structural diagram of a micro-channel temperature control device for nucleic acid detection.
FIG. 2 is a schematic diagram of a nucleic acid detection reaction module and a temperature control module.
FIG. 3 is a schematic diagram of the nucleic acid detection reaction module showing the thermal conductivity change.
FIG. 4 is a schematic view of a serpentine microchannel.
Detailed Description
The technical solutions of the present invention are further illustrated and described below with reference to specific embodiments, but the present invention is not limited to the embodiments.
Example 1
The device for controlling the temperature of the micro-channel for nucleic acid detection and amplification reaction shown in fig. 1 and 2 comprises a reaction module, a temperature rising/reducing module, a micro-channel cooling module and a microcontroller module. The reaction module comprises a reaction tube 1, a reaction column 2 and a reaction plate 3. The temperature rising/reducing module comprises a thermoelectric refrigerating piece 4, two power lines 5 are arranged on the thermoelectric refrigerating piece 4, and switching between the hot end and the cold end of the thermoelectric refrigerating piece can be realized by changing the positive and negative electrodes of the power lines. The reaction column 2 and the thermoelectric refrigerating piece 4 are respectively provided with thermocouples 8-2 and 8-1 for acquiring temperature signals, the acquired temperature signals are transmitted to the microcontroller module 13, and the microcontroller module 13 controls the current and the positive and negative poles of the thermoelectric refrigerating piece to realize temperature rise and fall. The micro-channel cooling module comprises a micro-channel 6, a liquid pipe 7, a cooling fan 9, a condenser 10, a liquid storage device 11 and a water pump 12; the outlet of the condenser 10 is provided with a thermocouple 8-5, as shown in fig. 4, the microchannel 6 comprises a microchannel plate 6-1 and a microchannel cover plate 6-2, the microchannel plate 6-1 is provided with 5 groups of snake-shaped microchannels 6-1(c) which are not communicated with each other, and an inlet 6-1(a) and an outlet 6-1(b) are arranged at the same time (the inlet and the outlet of the microchannel have the same function, do not influence the technical effect, and are respectively connected with the liquid pipe 7. a liquid level controller is arranged in the liquid storage device 11, a liquid level signal is transmitted to a microcontroller module 13 by a liquid level sensor 8-3 to control the amount of cooling water participating in circulation, the water pump is provided with a control circuit 8-4, the flow rate of the cooling water is controlled by the microcontroller, the reaction module is arranged above the temperature raising/lowering module, and the microchannel cooling module is arranged below the temperature raising/lowering module, the modules are installed in a stacked mode, heat transfer is facilitated, and heat-conducting silicone grease is coated among the modules, so that thermal contact resistance is reduced.
Further preferably, as shown in fig. 3, the reaction column 2 is installed in a manner that an outer layer is made of a low thermal conductivity material 2-1 and an inner layer is made of a high thermal conductivity material 2-2. In this embodiment, the low thermal conductivity material is aluminum alloy, and the high thermal conductivity material is red copper. The reaction plate 3 is of a multilayer structure nested inside and outside, wherein the outer layer is made of a low-thermal-conductivity material 3-1, and the inner layer is made of a high-thermal-conductivity material 3-2. In this embodiment, the low thermal conductivity material is aluminum alloy, and the high thermal conductivity material is red copper. The reaction plate 3 is provided with a hole 3-3 inside for installing the reaction column 2. The number of the holes can be 24 holes, 48 holes or 96 holes, and 24 holes are adopted in the embodiment.
The micro-channel temperature control device and the method for the nucleic acid detection and amplification reaction have the working principle that: when the PCR reaction starts, the microcontroller gives a forward voltage to the thermoelectric refrigerating sheet 4, and at the moment, the surface of the thermoelectric refrigerating sheet, which is in contact with the reaction plate 3, is a hot end, and the surface of the thermoelectric refrigerating sheet, which is in contact with the microchannel 6, is a cold end. Under the action of the Peltier effect, the heat of the cooling water in the micro-channel 6 is continuously released to the reaction module, meanwhile, the heat generated by the thermoelectric refrigerating sheet 4 in the working process is also transmitted to the reaction module, and the temperature of the reaction module rapidly rises to the rated upper temperature limit of 96 ℃. At the moment, the micro control module adjusts the current of the thermoelectric refrigerating sheet 4, so that the temperature of the reaction module is maintained at the rated temperature, and a high-temperature heat preservation link is carried out. After the heat preservation is finished, the microcontroller module 13 gives a reverse voltage to the thermoelectric refrigerating sheet 4, the cold end and the hot end of the thermoelectric refrigerating sheet 4 are switched, the heat of the reaction module is reversely transmitted to the micro-channel 6, and cooling water in the micro-channel is used as heat sink to store the heat. Because the inside of the microchannel 6 is provided with 5 groups of snake-shaped microchannels 6-1(c) which are not communicated with each other, the temperature uniformity in the extension direction and the width direction can be improved. The flow rate of the cooling water can be adjusted by the water pump 12 according to the amount of heat released by the thermoelectric cooling fins. When the temperature of the reaction module is reduced to 30 ℃ below the rated lower temperature limit, the microcontroller module adjusts the current of the thermoelectric refrigerating sheet 4 and enters a low-temperature heat preservation link. After the low-temperature heat preservation link is finished, one temperature rising/reducing cycle of the PCR reaction is finished, namely the next cycle is about to enter, and at the moment, the number of the nucleic acid in the reaction tube 1 is amplified by one time.
Since the thermoelectric cooling fins 4 generate a large amount of heat during operation, the cooling water absorbs more heat than the released heat during one cycle. At this time, the heat dissipation fan 9 is turned on to dissipate the excess heat into the air, and the system heat balance is maintained, so that the temperature at the beginning of the next temperature rising/lowering cycle is kept consistent with that of the previous cycle, and the temperature is detected by the thermocouple 8-5 installed at the outlet of the condenser 10 and transmitted to the microcontroller module 13. Because the heat exchange performance of the micro-channel 6 is good, heat can be absorbed or released by cooling water rapidly, therefore, the thermoelectric cooling plate 4 can select a model with larger power (such as PCR100145 can be selected, and the maximum cooling capacity reaches 177.1W), and the heating/cooling rate is increased effectively. In addition, cooling water is used as a heat sink, and a small amount of cooling water can meet the requirements of heat absorption and release due to large specific heat capacity; in a plurality of cycles, heat is transferred back and forth between the reaction module and the microchannel heat dissipation module, so that the temperature control method has the advantages of compact structure, energy conservation and emission reduction.
The reaction module adopts a gradient thermal conductivity material, the reaction plate adopts an internally and externally nested two-layer structure, the outer layer is aluminum alloy with low thermal conductivity of 3-1, and the inner layer is red copper with high thermal conductivity of 3-2; the outer layer reaction column 2-1 is made of aluminum alloy, and the inner layer reaction column 2-2 is made of red copper. The arrangement is equivalent to that a layer of heat insulation material is wrapped outside the reaction module, so that the heat exchange between the reaction module and the outside air is reduced, the temperature uniformity of the reaction module can be further improved, and the accuracy and the sensitivity of a nucleic acid detection result are improved.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. A micro-channel temperature control device for nucleic acid detection and amplification reaction is characterized by comprising a nucleic acid amplification reaction module, a temperature rising/reducing module, a micro-channel cooling module and a microcontroller module; the nucleic acid amplification reaction module and the micro-channel cooling module are respectively arranged on the upper surface and the lower surface of the temperature rising/reducing module;
the nucleic acid amplification reaction module comprises a reaction plate, a reaction column and a reaction tube, wherein the reaction column is arranged in a hole of the reaction plate and used for fixing the reaction column, and the reaction tube is sleeved in the reaction column; the reaction plate is of a multilayer structure nested inside and outside, and the heat conductivity from the outer layer to the inner layer is gradually increased; the heat conductivity of the reaction column from the outer layer to the inner layer is gradually increased; the internally and externally nested multilayer structure is made of stainless steel as a first thermal conductivity material, brass as a second thermal conductivity material, aluminum alloy as a third thermal conductivity material and red copper as a fourth thermal conductivity material;
the temperature rising/lowering module is a thermoelectric refrigerating sheet and is attached to the bottom of the reaction plate, and the temperature rising/lowering and temperature control of the reaction module can be realized through the microcontroller module;
the microchannel cooling module comprises a microchannel plate, a microchannel cover plate, a liquid pipe, a cooling fan, a condenser, a liquid reservoir and a water pump; the microchannel plate is provided with a microchannel, and an inlet and an outlet of the microchannel are respectively connected with the liquid pipe; the microchannel cover plate is arranged at the upper part of the microchannel plate, and the top of the microchannel cover plate is attached to the temperature rising/reducing module; one end of the liquid pipe is respectively connected with an inlet and an outlet of the microchannel, the other end of the liquid pipe is respectively connected to a liquid storage device through a water pump and a condenser, a water cooling device is stored in the liquid storage device, and a liquid level controller and a liquid level sensor are arranged in the liquid storage device, wherein the liquid level controller can regulate and control cooling water participating in circulation according to a thermal balance condition; the water pump is used for driving cooling water to circulate between the micro-channel and the condenser, and the cooling water flowing in the liquid pipe takes away heat; thermocouples are arranged at the outlets of the reaction column, the thermoelectric refrigerating sheet and the condenser and are used for collecting temperature signals, the water pump is provided with a voltage signal sensor, and the liquid storage device is provided with a liquid level signal sensor; the microchannel plate consists of a plurality of groups of snakelike microchannels which are not communicated with one another;
and the microcontroller module is used for receiving the sensor signal and sending an instruction to control the temperature rise/fall of the thermoelectric refrigerating sheet and the participation amount and circulation rate of the cooling water.
2. The apparatus according to claim 1, wherein the cooling water is stored in the reservoir, and a level controller is provided to control the cooling water circulating according to a thermal equilibrium condition.
3. The micro-channel temperature control device for nucleic acid detection and amplification reaction of claim 1, wherein the reaction plate is a 24-well, 48-well or 96-well reaction plate.
4. The device for controlling the temperature of the micro-channel for nucleic acid detection and amplification reaction of claim 1, wherein the temperature raising/lowering module employs a thermoelectric cooling plate.
5. The device for controlling the temperature of a microchannel in a nucleic acid detection amplification reaction according to claim 1, wherein the plurality of sets include 5 to 10 sets.
6. A method for controlling the temperature of a microchannel in a nucleic acid detection amplification reaction, which comprises the steps of:
1) when the PCR reaction starts, the microcontroller gives a forward voltage to the thermoelectric refrigerating sheet, and at the moment, one surface of the thermoelectric refrigerating sheet, which is in contact with the reaction plate, is a hot end, and the other surface of the thermoelectric refrigerating sheet, which is in contact with the microchannel, is a cold end; under the action of the Peltier effect, the heat of cooling water in the micro-channel is continuously released to the reaction module, meanwhile, the heat generated by the thermoelectric refrigerating sheet in the working process is also transmitted to the reaction module, and the temperature of the reaction module is rapidly increased to the rated upper temperature limit of 96 ℃; the micro-control module adjusts the current of the thermoelectric refrigerating sheet, so that the temperature of the reaction module is maintained at a rated temperature, and a high-temperature heat preservation link is carried out;
2) after the heat preservation is finished, the microcontroller module supplies a reverse voltage to the thermoelectric refrigerating sheet, the cold end and the hot end of the thermoelectric refrigerating sheet are switched, the heat of the reaction module is reversely transmitted to the microchannel, and cooling water in the microchannel is used as heat sink to store the heat;
3) when the temperature of the reaction module is reduced to 30 ℃ below the rated lower temperature limit, the microcontroller module adjusts the current of the thermoelectric refrigerating sheet and enters a low-temperature heat preservation link;
4) after the low-temperature heat preservation link is finished, finishing one temperature rising/reducing cycle of the PCR reaction, namely entering the next cycle, and amplifying the number of nucleic acid in the reaction tube by one time;
5) the cooling fan is started to radiate redundant heat into the air, the heat balance of the system is maintained, the temperature at the beginning of the next temperature rising/reducing cycle is consistent with that of the previous cycle, and the temperature is detected by a thermocouple arranged at the outlet of the condenser and is transmitted to the microcontroller module.
7. The method of claim 6, wherein in the step 2), the flow rate of the cooling water is adjusted by a water pump according to the amount of heat released from the thermoelectric cooling plate.
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| CN109929753B (en) * | 2019-03-14 | 2019-11-08 | 宁波胤瑞生物医学仪器有限责任公司 | A kind of blower fan module digitizing nucleic acid augmentative instrument |
| CN111229350A (en) * | 2019-09-27 | 2020-06-05 | 杭州柏恒科技有限公司 | Liquid cooling automatic PCR equipment |
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2020
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