CN206946338U - A kind of temperature control device applied to nucleic acid amplification technologies - Google Patents
A kind of temperature control device applied to nucleic acid amplification technologies Download PDFInfo
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- CN206946338U CN206946338U CN201720541280.6U CN201720541280U CN206946338U CN 206946338 U CN206946338 U CN 206946338U CN 201720541280 U CN201720541280 U CN 201720541280U CN 206946338 U CN206946338 U CN 206946338U
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- heat
- temperature
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- chilling plate
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Abstract
The utility model discloses a kind of temperature control device and control method applied to nucleic acid amplification technologies, the device includes hot lid, test tube, alternating temperature metal derby, heat-conducting silicone grease, semiconductor chilling plate, heat dissipation metal plate, heat abstractor, fan, rubber base plate, full H bridges drive module, controller, button, display screen, casing and temperature sensor；The utility model production cost is low；It is simple to operate, using wide, the whether PCR amplification techniques of early stage, or emerging LAMP and RPA amplification techniques, can be switched in this equipment by human-computer interaction interface or self-defined setting.
Description
Technical field
The utility model belongs to field of industrial automation control, and specifically a kind of temperature control applied to nucleic acid amplification technologies fills
Put.
Background technology
Nucleic acid amplification is the conventional research tool of life science, the core carried out using PCR (PCR)
Sour amplification in vitro is a revolutionary technology for starting nineteen eighty-three to grow up, and has been widely used the agricultural in modernization at present
With the field such as medical science and food industry, particularly during human cognitive gene and genome, beyond body nucleic acid amplification skill
Art is made that the contribution of brilliance.
Because normal PCR is limited by factors such as instrument and equipment and electric power and spaces all the time.Constant temperature PCR in recent years
Technology has begun to quietly rise.In past 10 years, some constant temperature nucleic acid amplification technologies are rapidly developed, as SDA technologies,
TMA technologies, HDA technologies, LAMP technology and RPA/RAA technologies etc..The characteristics of new technology is under isothermal conditions can efficiently, soon
Speed, height is special, expands target sequence with sensitivity.
Either classical, traditional pcr gene amplification technique still improve after new technology, the core of nucleic acid augmentative instrument is
One temperature control device, it can be required for different reactions to realize reactant in denaturation, renaturation, the temperature for extending different phase
With the control of time.And because temperature control system has hysteresis quality, time variation and nonlinear characteristic, while gene-amplificative instrament is to reality
Test that the accuracy requirement of temperature is also higher, therefore design and a kind of can automatically adjust temperature to meet that the equipment of experiment demand just shows
Obtain particularly important.
The content of the invention
The utility model is difficult for currently high, the complex operation on amplification instrument cost of manufacture, the not high technology of control accuracy
A kind of topic, it is proposed that temperature control device applied to nucleic acid amplification technologies.
A kind of temperature control device applied to nucleic acid amplification technologies, the device include hot lid, test tube, alternating temperature metal derby, heat conduction
Silicone grease, semiconductor chilling plate, heat dissipation metal plate, heat abstractor, fan, rubber base plate, full H bridges drive module, controller, press
Key, display screen, casing and temperature sensor；
The top of test tube is provided with heat and covered, and test tube the latter half is arranged in alternating temperature metal derby, on heat lid and alternating temperature metal derby
Provided with temperature sensor, alternating temperature metal derby bottom is provided with semiconductor chilling plate, and heat dissipation metal plate is provided with below semiconductor chilling plate,
Heat-conducting silicone grease is scribbled between alternating temperature metal derby and semiconductor chilling plate and between semiconductor chilling plate and heat dissipation metal plate；Radiating
The heat abstractor to be radiated by fan is provided with below sheet metal；The signal output part and controller of described temperature sensor
Temperature signal input connection, the driving signal output end of controller is connected with full H bridges drive module driving signal input,
The signal output termination semiconductor chilling plate of full H bridge drive modules；Described bottom half is provided with rubber base plate；The outside of casing
Face is provided with display screen and button；Described temperature sensor model DS18B20.
Compared with prior art, the utility model has following features：
1. production cost is low.With the continuous development of semiconductor fabrication process, from more efficient and practical designing material
Control cost of manufacture well.
2. it is simple to operate, using wide.Although nucleic acid amplification technologies constantly update development, its core technology needed does not have
Become.Whether the PCR amplification techniques of early stage, or emerging LAMP and RPA amplification techniques, can pass through in this equipment
Human-computer interaction interface switches over or self-defined setting.
3. control effect is more preferably.Faster response speed, smaller overshoot and stronger antijamming capability can be obtained.
Brief description of the drawings
Fig. 1 amplification instrument structural representations.
Embodiment
As shown in figure 1, a kind of temperature control device applied to nucleic acid amplification technologies, it is characterised in that：The device includes heat and covered
1st, test tube 2, alternating temperature metal derby 3, heat-conducting silicone grease 4, semiconductor chilling plate 5, heat dissipation metal plate 6, heat abstractor 7, fan 8, rubber
Base 9, full H bridges drive module 10, controller 11, button 12, display screen 13, casing 14 and temperature sensor 15；
The top of test tube 2 is provided with hot lid 1, and test tube the latter half is arranged in alternating temperature metal derby 3, hot lid 1 and alternating temperature metal
Block 3 is provided with temperature sensor 15, and the bottom of alternating temperature metal derby 3 is provided with semiconductor chilling plate 5, and the lower section of semiconductor chilling plate 5 is provided with
Heat dissipation metal plate 6, between alternating temperature metal derby 3 and semiconductor chilling plate 5 and between semiconductor chilling plate 5 and heat dissipation metal plate 6
Scribble heat-conducting silicone grease 4；The lower section of heat dissipation metal plate 6 is provided with the heat abstractor 7 to be radiated by fan 8；Described TEMP
The signal output part of device is connected with the temperature signal input of controller 11, and the driving signal output end of controller is driven with full H bridges
The driving signal input of dynamic model block 10 connects, the signal output termination semiconductor chilling plate of full H bridges drive module 10；Described case
The bottom of body 14 is provided with rubber base plate 9；The lateral surface of casing is provided with display screen 13 and button 12；Described temperature sensor model
For DS18B20.
Below exemplified by the PCR nucleic acid amplifications based on three-stage temperature requirements, to describe the work of temperature control device in detail
Process.
PCR amplification techniques are divided into three temperature control stages：
Preparatory stage：Design temperature (60-65 DEG C) is reached with the most fast time, to meet the condition of gene magnification.
The amplification stage：Holding isoperibol is constant, and single stranded DNA is expanded.
The renaturation stage：Environment temperature is risen to relevant temperature (98 DEG C), be steadily cooled to 80 DEG C, to realize denaturation
DNA regroups into double-strand helical structure.
Claims (2)
- A kind of 1. temperature control device applied to nucleic acid amplification technologies, it is characterised in that：The device includes hot lid, test tube, alternating temperature gold Belong to block, heat-conducting silicone grease, semiconductor chilling plate, heat dissipation metal plate, heat abstractor, fan, rubber base plate, full H bridges drive module, control Device, button, display screen, casing and temperature sensor processed；The top of test tube is provided with heat and covered, and test tube the latter half is arranged in alternating temperature metal derby, and heat lid and alternating temperature metal derby are provided with Temperature sensor, alternating temperature metal derby bottom are provided with semiconductor chilling plate, heat dissipation metal plate, alternating temperature are provided with below semiconductor chilling plate Heat-conducting silicone grease is scribbled between metal derby and semiconductor chilling plate and between semiconductor chilling plate and heat dissipation metal plate；Heat radiating metal The heat abstractor to be radiated by fan is provided with below piece；The signal output part of described temperature sensor and the temperature of controller Signal input part connection is spent, the driving signal output end of controller is connected with full H bridges drive module driving signal input, full H The signal output termination semiconductor chilling plate of bridge drive module；Described bottom half is provided with rubber base plate；The lateral surface of casing It is provided with display screen and button.
- A kind of 2. temperature control device applied to nucleic acid amplification technologies according to claim 1, it is characterised in that：Described temperature Degree sensor model number is DS18B20.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720541280.6U CN206946338U (en) | 2017-05-16 | 2017-05-16 | A kind of temperature control device applied to nucleic acid amplification technologies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720541280.6U CN206946338U (en) | 2017-05-16 | 2017-05-16 | A kind of temperature control device applied to nucleic acid amplification technologies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206946338U true CN206946338U (en) | 2018-01-30 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720541280.6U Expired - Fee Related CN206946338U (en) | 2017-05-16 | 2017-05-16 | A kind of temperature control device applied to nucleic acid amplification technologies |
Country Status (1)
| Country | Link |
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| CN (1) | CN206946338U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107015584A (en) * | 2017-05-16 | 2017-08-04 | 杭州电子科技大学 | A kind of temperature control device and control method applied to nucleic acid amplification technologies |
| CN111949055A (en) * | 2020-08-03 | 2020-11-17 | 中国科学院长春光学精密机械与物理研究所 | Independent temperature control system and method for microfluidic culture chip |
| CN112063491A (en) * | 2020-09-15 | 2020-12-11 | 厦门大学 | Micro-channel temperature control device and method for nucleic acid detection amplification reaction |
-
2017
- 2017-05-16 CN CN201720541280.6U patent/CN206946338U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107015584A (en) * | 2017-05-16 | 2017-08-04 | 杭州电子科技大学 | A kind of temperature control device and control method applied to nucleic acid amplification technologies |
| CN111949055A (en) * | 2020-08-03 | 2020-11-17 | 中国科学院长春光学精密机械与物理研究所 | Independent temperature control system and method for microfluidic culture chip |
| CN112063491A (en) * | 2020-09-15 | 2020-12-11 | 厦门大学 | Micro-channel temperature control device and method for nucleic acid detection amplification reaction |
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Legal Events
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180130 Termination date: 20210516 |