CN209307379U - A kind of PCR thermostatic platform based on Arduino platform courses - Google Patents

Abstract

The utility model discloses a kind of PCR thermostatic platforms based on Arduino platform courses, including PCR micro-fluid chip, semiconductor chilling plate, temperature collecting module, Arduino single-chip microcontroller, LCD display and keyboard, wherein, the Arduino single-chip microcontroller is core component, it is used to the analog quantity temperature value of temperature collecting module real-time temperature values of collected PCR micro-fluid chip in real time being converted to digital quantity temperature value, and show digital quantity temperature value on an lcd display, it can intuitively obtain real time temperature；Setting control temperature value is adjusted by Keyboard Control, then is compared by Arduino single-chip microcontroller with the digital quantity temperature value of acquisition, to control the heating or refrigeration of semiconductor chilling plate, to reach the accurate constant control of the temperature of PCR micro-fluid chip.The utility model is easy to operate, safe and reliable, can satisfy overall performance requirement, has a good application prospect and promotional value.

Description

A kind of PCR thermostatic platform based on Arduino platform courses

Technical field

The utility model relates to automatic technologies and information technology field, and in particular to one kind is based on the control of Arduino platform The PCR thermostatic platform of system.

Background technique

PCR micro-fluidic chip is third generation round pcr, and the technology is by template DNA, primer, Tag enzyme, buffer according to elder generation The micro- reactive tank processed on shaped like chips solid support is sequentially added afterwards, is uniformly mixed；Then PCR mixture is allowed to iterate through Three constant temperature zones [degeneration area Melting (94 DEG C), annealed zone Annealing (55 DEG C), amplification region on chip Extension (72 DEG C)], to realize the denaturation of DNA, annealing and amplification.Each circulate through so is denaturalized, anneals and prolongs It stretches, DNA content doubles.The temperature controlled order of accuarcy of three warm areas is the key factor for completing PCR amplification.To mesh Before until the round pcr based on chip there are mainly two types of implementation pattern: one is the pipe sections that reagent changes in temperature cycles In continuously flowing realize PCR amplification, referred to as continuously flowing PCR microchip；Another kind is that reagent is motionless, and the temperature of chip cavity Rapid circulation change realizes PCR amplification, referred to as PCR micro-chamber chip.Former mode is easy to accomplish integrated, latter approach Reaction speed is faster than former.

The prior art is mostly ito thin film heating (design of Liu Chao, Yang Sheng PCR micro-fluid chip temperature control system), instead Answer speed relatively slow, and this programme is by raising and reducing to control temperature semiconductor refrigeration chip heating or refrigeration, energy The rapid circulation change of temperature is realized to realize PCR amplification, reaction speed is fast, at low cost, Yi Shixian.

Utility model content

The purpose of this utility model is that: expand PCR microfluid by adjusting control temperature to semiconductor chilling plate and PID Degree of heating is more accurate, precision is higher, gradient of temperature responds faster.

The technical solution adopted in the utility model are as follows: a kind of PCR thermostatic platform based on Arduino platform courses, including PCR Micro-fluid chip, semiconductor chilling plate, temperature collecting module, Arduino single-chip microcontroller, LCD display and keyboard, wherein described Arduino single-chip microcontroller is core component, is used for the real-time temperature of temperature collecting module collected PCR micro-fluid chip in real time The analog quantity temperature value of angle value is converted to digital quantity temperature value, and digital quantity temperature value is shown on an lcd display, Neng Gouzhi It sees and obtains real time temperature；Setting control temperature value is adjusted by Keyboard Control, then passes through the number of Arduino single-chip microcontroller and acquisition Amount temperature value is compared, controlling the heating or refrigeration of semiconductor chilling plate, to reach the temperature of PCR micro-fluid chip Accurate constant control.

Wherein, Arduino single-chip microcontroller carries out temperature acquisition by thermocouple sensor, passes through MAX6675 data converter Celsius temperature is converted by the K-type electric thermo-couple temperature of acquisition and is transmitted to single-chip microcontroller, and single-chip microcontroller is by software control on the one hand by temperature Real-time display on the lcd screen, on the other hand will read temperature to sensor and be compared with set temperature, and Δ=sensor is read Temperature-set temperature；If Δ < 0, Arduino D6 pin exports high level, Relay1 work makes Relay1 form positive electricity Road is pushed back, i.e. semiconductor chilling plate carries out heated condition；If Δ > 0, Arduino D10 pin exports high level, Relay2 work Make, Relay2 is made to form backward voltage circuit, is i.e. semiconductor chilling plate should carry out refrigeration work state at this time；Single-chip microcontroller is not by Disconnected temperature collection simultaneously carries out real time temperature control compared with set temperature, so that fast accurate reaches set temperature；Herein Period needs heat when semiconductor chilling plate working by the water circulated to take away, to reduce energy consumption；It can also be straight Connection overregulates semiconductor chilling plate TEC1-12706 input voltage, electric current to control its refrigeration or heating relative velocity；Pass through Pid control algorithm can make temperature error reach minimum.

Wherein, PCR micro-fluidic temperature is controlled by Arduino platform and semiconductor chilling plate, to realize micro- to PCR The amplification of fluid.

The utility model advantage and good effect are as follows:

The utility model proposes a kind of temperature control system of PCR micro-fluid chip based on Arduino single-chip microcontroller, this System controls the element circuit etc. for the Switching Power Supply for having power dissipation, thus the semiconductor chilling plate working condition of control (refrigerating/heating conversion, watt level is adjustable) guarantees what it was worked normally to reach measurement and control the temperature of PCR microfluid Purpose.It is adjusted and is controlled by PID, single temperature range error can drop to Δ=± 0.5 DEG C, the system operatio side by Δ=± 2 DEG C Just, safe and reliable, it can satisfy overall performance requirement, have a good application prospect and promotional value.

Detailed description of the invention

Fig. 1 is a kind of functional block diagram of the PCR thermostatic platform based on Arduino platform courses of the utility model；

Fig. 2 is the design schematic diagram of the temperature control system of the PCR micro-fluid chip based on Arduino single-chip microcontroller.

Specific embodiment

With reference to the accompanying drawing and specific embodiment further illustrates the utility model.

The utility model is when solving accurate temperature control technique based on several modules as shown in Figure 1: in Arduino single-chip microcontroller Under control, comparison is done to control the heating or refrigeration of semiconductor chilling plate, to control with setting temperature by the acquisition to temperature The amplification of PCR micro-fluid chip processed.LCD module circuit can show real time temperature.It is controlled by Arduino single-chip microcontroller master control borad The temperature realization of PCR micro-fluid chip accurately expands rapidly PCR micro-fluid chip.Specifically by nickel chromium triangle/nisiloy K-type thermoelectricity Even sensor and MAX6675K type Thermocouple module acquisition PCR micro-fluid chip real time temperature, acquisition come temperature with The control temperature of Arduino program compares, if temperature is lower than set temperature, passes through relay module and semiconductor refrigerating Piece to carry out computer heating control to PCR micro-fluid chip., whereas if temperature collection is higher than set temperature, then pass through relay mould Block and semiconductor chilling plate to carry out refrigeration control to PCR micro-fluid chip.Wherein need to semiconductor chilling plate provide 12V, 5A D.C. regulated power supply is adopted as the WYJ.5A 30V DC REYILATED POWER SUPPLYDOUBLE WAY of WYJ series OUTPUT.Conventional scheme is fan for cooling technology, is radiated using Water Cooling Technology, because water cooling is without friction compared to fan, compares Quieter, reduction noise.Semiconductor chilling plate can be carried out heat when refrigeration work by water-bath simultaneously by the water that circulates Amount is taken away, and energy consumption is reduced.Radiate while cooperating jointly with peristaltic pump achieving the purpose that cool down rapidly using water-bath system.Most After increase LCD screen, effect is the real time temperature that can be visually seen acquisition PCR micro-fluid chip.

The utility model takes the PCR as shown in Figure 2 based on Arduino single-chip microcontroller micro- when solving accurate temperature control technique The temperature control system of fluid chip obtains design schematic diagram overall technical architecture.

Code name 1 is Arduino single-chip microcontroller, and selected model ARDUINO UNO R3 development board official version modified version is supported WIN7WIN10.Digital I/O numeral input/output end totally 0~13.Analog I/O numeral input/output end totally 0~5. Output voltage: 5V direct voltage output and 3.3V direct voltage output.Using Atmel Atmega328 microprocessor controller.

Code name 2 is that MAX6675K type Thermocouple module is the production of U.S.'s MAXIM company with cold junction compensation, linear school Just, the serial K-type thermocouple analog-digital converter of 12 bit resolutions of thermocouple burn out detection.Simple SPI serial port temperature value is defeated Out, 0 DEG C~1024 DEG C of temperature-measuring range, converter temperature resolution ratio are 0.25 DEG C.Supply voltage is 3~5V DC.

SO, CS and SCK connect D7, D8 and D9 pin of Arduino respectively.

Code name 3 is K-type thermocouple sensor.Core material is nickel chromium triangle/nisiloy, and red be cathode/Huang is that anode connects On the positive and negative anodes of MAX6675.

Code name 4 is LCD1602 blue screen band backlight liquid crystal screen.D4~D7 of LCD successively meets D5~D2 of Arduino, LCD's E pin and RS pin meet the D11 and D12 of Arduino respectively.

Code name 5 is TEC1-12706 semiconductor chilling plate.Voltage rating 12V, Imax=4~4.6A (when specified 12V), most Big 67 DEG C of temperature difference or more.The end NO of anode difference contact relay modular circuit Relay1 and Relay2, cathode distinguish contact relay The end NC of modular circuit Relay1 and Relay2.

Code name 6 is Relay1, and low and high level triggering, operating voltage 5V are supported in 1 tunnel relay module band light-coupled isolation. COM port meets external dc power 12V, and the port IN connects the port D6 of Arduino.

Code name 7 is Relay2, and low and high level triggering, operating voltage 5V are supported in 1 tunnel relay module band light-coupled isolation. COM port meets the GND of external dc power, and the port IN connects the port D10 of Arduino.

Working principle: Arduino single-chip microcontroller carries out temperature acquisition by thermocouple sensor, is turned by MAX6675 data Parallel operation converts Celsius temperature for the K-type electric thermo-couple temperature of acquisition and is transmitted to single-chip microcontroller, on the one hand single-chip microcontroller will by software control Temperature real-time display on the lcd screen, on the other hand will read temperature to sensor and be compared with set temperature, Δ=sensor Read temperature-set temperature.If Δ < 0, Arduino D6 pin exports high level, Relay1 work forms Relay1 just To voltage circuit, i.e. semiconductor chilling plate carries out heated condition；If Δ > 0, Arduino D10 pin exports high level, Relay2 work, makes Relay2 form backward voltage circuit, i.e. semiconductor chilling plate should carry out refrigeration work state at this time.Monolithic Machine carries out real time temperature control by continuous temperature collection and compared with set temperature, so that fast accurate reaches setting temperature Degree.Heat when semiconductor chilling plate working by the water circulated is needed to take away during this period, to reduce energy consumption；? Its refrigeration directly can be controlled by adjusting semiconductor chilling plate TEC1-12706 input voltage, electric current or heating is relatively fast Degree.Temperature error can be made to reach minimum by pid control algorithm.

This programme is by utilizing Arduino single-chip microcontroller, thermocouple sensor, MAX6675 data converter, copper billet, relay Device module (Relay1 and Relay2), relay module Relay1 and Relay2 input pin connect Arduino single-chip microcontroller respectively D6 and D10 pin, water-bath cooling system, LCD module show the elements such as real-time sensor temperature and semiconductor chilling plate TEC A whole set of has been built for accurately controlling the design of PCR micro-fluid chip temperature system.

By semiconductor chilling plate, it is with the heating or refrigeration of Arduino software programming control PCR micro-fluid chip Specific protection scope, other schemes are ito thin film heating.

This programme proposes the dress of the temperature control in the micro-fluid chip control system that a kind of structure is simple, performance is good, at low cost It sets, for the temperature regulating device using commercially available semiconductor chilling plate as heater, apparatus structure is simple, small in size, and thermal capacitance is small, precision It is high, warming and cooling rate is fast, temperature precise control, the advantages that dynamic process is steady.

Claims (3)

1. a kind of PCR thermostatic platform based on Arduino platform courses, it is characterised in that: including PCR micro-fluid chip, semiconductor Cooling piece, temperature collecting module, Arduino single-chip microcontroller, LCD display and keyboard, wherein the Arduino single-chip microcontroller is core Center portion part is used for the simulation amount temperature of temperature collecting module real-time temperature values of collected PCR micro-fluid chip in real time Value is converted to digital quantity temperature value, and digital quantity temperature value is shown on an lcd display, can intuitively obtain real time temperature； Setting control temperature value is adjusted by Keyboard Control, then is compared by Arduino single-chip microcontroller and the digital quantity temperature value of acquisition Compared with to control the heating or refrigeration of semiconductor chilling plate, to reach the accurate constant control of the temperature of PCR micro-fluid chip.

2. a kind of PCR thermostatic platform based on Arduino platform courses according to claim 1, it is characterised in that: Arduino single-chip microcontroller carries out temperature acquisition by thermocouple sensor, by MAX6675 data converter that the K-type of acquisition is hot Galvanic couple temperature inversion is that Celsius temperature is transmitted to single-chip microcontroller, and single-chip microcontroller is by software control on the one hand by temperature real-time display in LCD On screen, on the other hand temperature being read to sensor and being compared with set temperature, Δ=sensor reads temperature-setting temperature Degree；If Δ < 0, Arduino D6 pin exports high level, Relay1 work makes Relay1 form forward voltage circuit, i.e., half Conductor cooling piece carries out heated condition；If Δ > 0, Arduino D10 pin exports high level, Relay2 work makes Relay2 Backward voltage circuit is formed, i.e. semiconductor chilling plate should carry out refrigeration work state at this time；Single-chip microcontroller passes through constantly acquisition temperature It spends and carries out real time temperature control compared with set temperature, so that fast accurate reaches set temperature；It needs to lead to during this period It crosses the heat when water circulated works semiconductor chilling plate to take away, to reduce energy consumption；Or directly by adjusting half Conductor cooling piece TEC1-12706 input voltage, electric current come control its refrigeration or heating relative velocity；It can by pid control algorithm Temperature error is set to reach minimum.

3. a kind of PCR thermostatic platform based on Arduino platform courses according to claim 1, it is characterised in that: pass through Arduino platform and semiconductor chilling plate control PCR micro-fluidic temperature, to realize the amplification to PCR microfluid.